CN103436357A - Preparation method of rana temporaria chensinensis egg oil without fishy smell - Google Patents
Preparation method of rana temporaria chensinensis egg oil without fishy smell Download PDFInfo
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- CN103436357A CN103436357A CN2013103231982A CN201310323198A CN103436357A CN 103436357 A CN103436357 A CN 103436357A CN 2013103231982 A CN2013103231982 A CN 2013103231982A CN 201310323198 A CN201310323198 A CN 201310323198A CN 103436357 A CN103436357 A CN 103436357A
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Abstract
The invention relates to a preparation method of rana temporaria chensinensis egg oil without a fishy smell, belongs to the technical field of medicinal biology, and aims at providing a preparation method for eliminating the fishy smell and removing odor of the chensinensis egg oil. The preparation method is high in operability, and is suitable for industrialization production and mass production, and no inflammable or explosive organic solvent exists. The preparation method adopts the technical scheme that the preparation method comprises the steps that dried rana temporaria chensinensis eggs are taken, smashed, and screened by a 50-200 mesh sieve; 0.1-5% of black tea aqueous solution and 0.1-5% of sodium chloride solution are added to an appropriate container, soaked at 0-50 DEG C for 10-300min, and removed by filtering; 0.1-5% of fungus solution is added, and subjected to heat preservation at 0-50 DEG C for 10-300min; sediments are taken, dried, and subjected to extraction separation by a CO2 supercritical extraction device by taking alcohol as an entrainer; rana temporaria chensinensis egg oil in a separation tank stands, and then is filtered; alcohol is recovered by pressure reduction; vacuum drying is performed; and the finished rana temporaria chensinensis egg oil is obtained.
Description
Technical field
The present invention relates to a kind of preparation method of woodfrog egg oil, particularly relate to a kind of preparation method of the woodfrog egg oil without fishy smell, belong to the medical biotechnology field.
Background technology
Rana temporaria chensinensis David (Rana temporaria chensinensis) is commonly called as frog, Oviductus Ranae, snow Kazakhstan etc., belong to amphibia, Anura, Ranidae, Rana on animal classification, it is the peculiar kind at the foot of a hill or mountain, Changbai Mountain, China the Northeast, belong to medicine, eat dual-purpose precious frog kind, and aboundresources.Wood frog oviduct is the famous and precious tonic Chinese medicine Oviductus Ranae of China's tradition, has the effect of nourishing the lung and kidney.Research in recent years shows again, the frog's egg of wood frog also contains the compositions such as the amino acid, trace element, polyunsaturated fatty acid, phosphide of needed by human, there is very high nutritive value, can be applicable to pharmacy and protective foods, there is the effect of regulating blood fat, delay senility and prevent climacteric syndrome, among the people, be acknowledged as the excellent tonic product arranged side by side with wood frog oviduct.And employing alcohol solvent or CO
2the technology such as supercritical extraction are extracted the wood frog ovum lipid-soluble substance obtained, and are commonly called as woodfrog egg oil, are considered to have in recent years lipopenicillinase, anxiety and Gynecological health isoreactivity.But, owing to itself having stronger fishy smell, mouthfeel is not good, can only make the preparation uses such as soft capsule, greatly limited its widespread use.
Summary of the invention
One of purpose of the present invention is to provide a kind of de-raw meat and taste removal preparation method of woodfrog egg oil; Purpose of the present invention two in provide woodfrog egg oil workable, there is no inflammable and explosive organic solvent, be suitable for industrialization and produce the large de-raw meat preparation method who produces.
The objective of the invention is to be achieved through the following technical solutions:
Get dry Rana temporaria chensinensis David ovum, pulverize, cross the 50-200 order, in appropriate containers, add the 0.1-5% black tea aqueous solution and 0.1-5% sodium chloride solution, soak 10-300 minute at 0 ℃-50 ℃, remove by filter tea and sodium chloride solution, then add the bacterium solution of 0.1-5%, at 0 ℃-50 ℃ insulation 10-300 minute, get precipitation, drying, do entrainment agent with ethanol, through CO
2supercritical extraction unit extracts and separates, by the standing rear filtration of the woodfrog egg oil in separating tank, and decompression recycling ethanol, vacuum-drying, obtain the finished product woodfrog egg oil.
Described bacterium solution, can be yeast solution, can be also Bacillus subtillis solution or milk-acid bacteria solution.
Compared with prior art, the resulting too equipment of complexity that do not need, do not need through resin absorption and the complicated like this treating process of molecular distillation yet in the present invention, and therefore de-fishy smell process grease loses little, simple to operate, can realize suitability for industrialized production.Add microorganism, in the process of microbial enzyme metabolism, removed woodfrog egg oil itself with offending fishy smell material, microbial strains used is the edible bacterial classification, harmless.
Following experimental example further illustrates the present invention, but does not act on limitation of the invention.
Embodiment 1:
Get dry Rana temporaria chensinensis David ovum 3kg, pulverize, cross 100 orders, in appropriate containers, add the 2% black tea aqueous solution and 1% sodium chloride solution, at 20 ℃, soak 100 minutes, remove by filter tea and sodium chloride solution, then add 3% yeast solution, 25 ℃ of insulations 100 minutes, get precipitation, drying, do entrainment agent with ethanol, through CO
2supercritical extraction unit extracts and separates, by the standing rear filtration of the woodfrog egg oil in separating tank, and decompression recycling ethanol, vacuum-drying, obtain the finished product woodfrog egg oil, yield 9.7%.
Embodiment 2:
Get dry Rana temporaria chensinensis David ovum 5kg, pulverize, cross 150 orders, in appropriate containers, add the 3% black tea aqueous solution and 2% sodium chloride solution, at 30 ℃, soak 150 minutes, remove by filter tea and sodium chloride solution, then add 0.5% Bacillus subtillis solution, 28 ℃ of insulations 150 minutes, get precipitation, drying, do entrainment agent with ethanol, through CO
2supercritical extraction unit extracts and separates, by the standing rear filtration of the woodfrog egg oil in separating tank, and decompression recycling ethanol, vacuum-drying, obtain the finished product woodfrog egg oil, yield 13.5%.
Embodiment 3:
Get dry Rana temporaria chensinensis David ovum 5kg, pulverize, cross 200 orders, in appropriate containers, add the 1% black tea aqueous solution and 1% sodium chloride solution, at 25 ℃, soak 250 minutes, remove by filter tea and sodium chloride solution, then add 3% milk-acid bacteria solution, 37 ℃ of insulations 200 minutes, get precipitation, drying, do entrainment agent with ethanol, through CO
2supercritical extraction unit extracts and separates, by the standing rear filtration of the woodfrog egg oil in separating tank, and decompression recycling ethanol, vacuum-drying, obtain the finished product woodfrog egg oil, yield 15.5%.
Above-described embodiment, the present invention embodiment a kind of more preferably just, those skilled in the art is in technical scheme scope of the present invention, and the common variation of carrying out and replacing all should be included in protection scope of the present invention.
Claims (4)
1. the preparation method without the fishy smell Rana temporaria chensinensis David egg oil, is characterized in that: get dry Rana temporaria chensinensis David ovum, pulverize, cross the 50-200 order, add the 0.1-5% black tea aqueous solution and 0.1-5% sodium chloride solution in appropriate containers, at 0 ℃-50 ℃, soak 10-300 minute, remove by filter tea and sodium chloride solution, the bacterium solution that adds again 0.1-5%, at 0 ℃-50 ℃ insulation 10-300 minute, get precipitation, drying, do entrainment agent with ethanol, through CO
2supercritical extraction unit extracts and separates, by the standing rear filtration of the woodfrog egg oil in separating tank, and decompression recycling ethanol, vacuum-drying, obtain the finished product woodfrog egg oil.
2. by preparation method claimed in claim 1, it is characterized in that bacterium solution can be yeast solution, concentration is 0.1-5%.
3. by preparation method claimed in claim 1, it is characterized in that bacterium solution can be Bacillus subtillis solution, concentration is 0.1-5%.
4. by preparation method claimed in claim 1, it is characterized in that bacterium solution can be milk-acid bacteria solution, concentration is 0.1-5%.
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| CN2013103231982A CN103436357A (en) | 2013-07-29 | 2013-07-29 | Preparation method of rana temporaria chensinensis egg oil without fishy smell |
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| CN2013103231982A CN103436357A (en) | 2013-07-29 | 2013-07-29 | Preparation method of rana temporaria chensinensis egg oil without fishy smell |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103750001A (en) * | 2013-12-20 | 2014-04-30 | 吉林市长白加林特产开发有限公司 | Formula and preparation method of instant type granule electuary made of wood-frog spawn |
| CN104312722A (en) * | 2014-09-16 | 2015-01-28 | 舟山龙鸿海洋生物开发有限公司 | Method for removing color and fishlike smell of fish meal processing by-product fish oil |
| CN105105126A (en) * | 2015-09-10 | 2015-12-02 | 北京碧延堂生物科技有限公司 | Fishy smell removing freeze-dried instant rana japonica oil and preparation method thereof |
| CN105919134A (en) * | 2016-04-22 | 2016-09-07 | 吉林大学 | Method for assisting in extracting wood frog spawn oil by ultrasonic method and preparation method of wood frog spawn oil microcapsules |
| CN109554217A (en) * | 2018-12-14 | 2019-04-02 | 福建铭发水产开发有限公司 | A method of surplus material production polyunsaturated fatty acid is processed using sea eel |
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| KR20010037770A (en) * | 1999-10-19 | 2001-05-15 | 정회함 | Health Food by BullFrog |
| CN1343496A (en) * | 2001-06-15 | 2002-04-10 | 沈阳药科大学 | Forest frog's egg fat injection and its preparing process |
| CN1346877A (en) * | 2001-04-28 | 2002-05-01 | 沈阳药科大学 | Process for super-critical extracting oil of forest frog ovum |
| CN1557206A (en) * | 2004-01-14 | 2004-12-29 | 江南大学 | Low value fresh water fish whole utilization process method |
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| CN102311878A (en) * | 2010-07-08 | 2012-01-11 | 吉林博大农林生物科技有限公司 | Chinese forest frog composite essence processing method |
| CN102423087A (en) * | 2011-11-24 | 2012-04-25 | 江南大学 | Vacuum microwave segmented combined drying manufacturing method of instant crisp dehydrated fish ball |
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Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| KR20010037770A (en) * | 1999-10-19 | 2001-05-15 | 정회함 | Health Food by BullFrog |
| CN1346877A (en) * | 2001-04-28 | 2002-05-01 | 沈阳药科大学 | Process for super-critical extracting oil of forest frog ovum |
| CN1343496A (en) * | 2001-06-15 | 2002-04-10 | 沈阳药科大学 | Forest frog's egg fat injection and its preparing process |
| CN1557206A (en) * | 2004-01-14 | 2004-12-29 | 江南大学 | Low value fresh water fish whole utilization process method |
| CN1563308A (en) * | 2004-04-22 | 2005-01-12 | 沈阳药科大学 | Antianxity effect of egg of Rana Cnensinensis and preparation method |
| CN102311878A (en) * | 2010-07-08 | 2012-01-11 | 吉林博大农林生物科技有限公司 | Chinese forest frog composite essence processing method |
| CN102423087A (en) * | 2011-11-24 | 2012-04-25 | 江南大学 | Vacuum microwave segmented combined drying manufacturing method of instant crisp dehydrated fish ball |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103750001A (en) * | 2013-12-20 | 2014-04-30 | 吉林市长白加林特产开发有限公司 | Formula and preparation method of instant type granule electuary made of wood-frog spawn |
| CN104312722A (en) * | 2014-09-16 | 2015-01-28 | 舟山龙鸿海洋生物开发有限公司 | Method for removing color and fishlike smell of fish meal processing by-product fish oil |
| CN105105126A (en) * | 2015-09-10 | 2015-12-02 | 北京碧延堂生物科技有限公司 | Fishy smell removing freeze-dried instant rana japonica oil and preparation method thereof |
| CN105919134A (en) * | 2016-04-22 | 2016-09-07 | 吉林大学 | Method for assisting in extracting wood frog spawn oil by ultrasonic method and preparation method of wood frog spawn oil microcapsules |
| CN109554217A (en) * | 2018-12-14 | 2019-04-02 | 福建铭发水产开发有限公司 | A method of surplus material production polyunsaturated fatty acid is processed using sea eel |
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Application publication date: 20131211 |
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