CN104069172B - Perilla leaf extract and its extracting method - Google Patents
Perilla leaf extract and its extracting method Download PDFInfo
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- CN104069172B CN104069172B CN201410313498.7A CN201410313498A CN104069172B CN 104069172 B CN104069172 B CN 104069172B CN 201410313498 A CN201410313498 A CN 201410313498A CN 104069172 B CN104069172 B CN 104069172B
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Abstract
The invention discloses a kind of perilla leaf extract and its extracting methods.The perilla leaf extract, by mass percentage, 1.25 parts of the active constituent B extracted comprising 1 part of active components A first extracting and again.After extracting A, through polyphenol, the pigment glycosides in microwave method removal perilla leaf.The present invention be made each ingredient of perilla leaf extract close to perilla leaf oils ratio, the ratio of especially perillaldehyde and perillene is higher.
Description
Technical field
The present invention relates to a kind of perilla leaf extract and its extracting methods, belong to the chemical extraction technique of plant.
Background technique
The main component of perilla leaf extract has perillaldehyde, firpene He limonene etc., can be used for deploying cosmetics, toothpaste and food
Product essence.01107031.5 is related to the method for extracting perilla leaf extract from purple perilla seed, including screening impurity elimination, raw material dehydration, pressure
Squeezing, filtering, degumming, depickling, de- soap, cleaning and disinfecting, crude oil be dehydrateds, sterilize, cooling down, and degumming depickling, de- soap, takes off
Water each process carries out between 40-60 DEG C of room temperature.The prior art also provides some perilla leafs that extract from perilla leaf and extracts
The method of object." response phase method optimizes Microwave Extraction perilla leaf polyphenol substance " (Wang Gennv) is disclosed and is extracted polyphenol from perilla leaf
The method of substance." extraction and analysis of perilla leaf anthocyanin and its research of function " (Cai Ningchen) is related to extracting flower from perilla leaf
The method of color glycosides.Single method extract perilla leaf more or less damage another part ingredient, the perilla leaf extract extracted at
It is point single, the ratio of each ingredient of perilla leaf extract cannot be restored.It is necessary to propose new extracting method, guarantee perilla leaf volatilization
The abundant extraction of oil, especially perillaldehyde and perillene sufficiently extract.
Summary of the invention
The present invention proposes a kind of perilla leaf extract and its extracting method, it is ensured that volatility oils, especially purple perilla
Aldehyde and perillene sufficiently extract.
In order to solve the above technical problems, the present invention constructs following technical proposals: weight percent is 0.4-0.5%'s
A kind of extracting method of perilla leaf extract, which comprises the following steps:
The perilla leaf for taking 100g mass after impregnating 10h in the solution of 1L, is cleaned, is dry；
It crushes, adds 1%HCl-70% ethyl alcohol 160g, stand 1h and extract 100g's after 25KHz ultrasonication 40min
Supernatant liquid extracts 4g active components A after macroporous resin purification；
Remaining perilla leaf is collected, 120g is dried to, 60% ethanol solution 200g, microwave power 200W is added, extracts 20s, mistake
Filter, removes liquid, filtered perilla leaf is dried to 110g；
In remaining perilla leaf be added 0.8%HCl-60% ethyl alcohol 150g, stand 1h, filtering, remove slag, 130 DEG C,
4h is distilled under 0.25Mpa, obtains the active constituent B of 3g,
By 1:1.25 mixing A, B, perilla leaf extract is made.
In the extracting method of perilla leaf extract of the invention, the solute of the solution is consisted of the following compositions:
The NH of NaCl, 2mmol of HCl, 8mmol of 13mmol4H2PO4, 1.5mmol H3BO3, citric acid 0.6mmol.In the solution
After immersion, it is convenient for post-processing.
In the extracting method of perilla leaf extract of the invention, perilla leaf is ground into diameter in 5mm or less.
In the extracting method of perilla leaf extract of the invention, ultrasonic power is 150W to 400W.
In the extracting method of perilla leaf extract of the invention, the macroreticular resin uses following methods to be made: with benzene
Ethylene, divinylbenzene, methacrylate are raw material, in 0.5% gelatin solution, dimethylbenzene are added and is polymerized.
In the extracting method of perilla leaf extract of the invention, the mass ratio of each component is styrene: divinylbenzene: first
Base acrylate: gelatin solution: dimethylbenzene=1:0.7:1.2:20:0.8.
A kind of perilla leaf extract, which is characterized in that by mass percentage, include 1 part of active components A, 1.25 parts
Active constituent B.
The present invention be made each ingredient of perilla leaf extract close to perilla leaf oils ratio, especially perillaldehyde and purple
The ratio of Soviet Union's alkene is higher.Polyphenol substance and pigment glycoside substance content in perilla leaf are higher, and withdrawal ratio is also higher, the present invention
After extracting A, through polyphenol, the pigment glycosides in microwave method removal perilla leaf, the ratio of polyphenol, pigment glycoside substance can be reduced.
Specific embodiment
As used in the description, the present invention is smaller to test parameters restriction range, only provides one embodiment below.Embodiment
In the item that is not described in detail referring to Chemistry for Chinese Traditional Medicine field common process.
The method of perilla leaf extract is extracted from perilla leaf, comprising the following steps:
The perilla leaf for taking 100g mass after impregnating 10h in the solution of 1L, is cleaned, is dry, the solute of the solution by with
It is lower at being grouped as: the NH of NaCl, 2mmol of HCl, 8mmol of 13mmol4H2PO4, 1.5mmol H3BO3, citric acid
0.6mmol。
Diameter is ground into 5mm hereinafter, addition 1%HCl-70% ethyl alcohol 160g, stands 1h, the ultrasonic wave through 25KHz, 300W
After handling 40min, the supernatant liquid of 100g is extracted, after macroporous resin purification, extracts 4g active components A.
Remaining perilla leaf is collected, 120g is dried to, 60% ethanol solution 200g, microwave power 200W is added, extracts 20s, mistake
Filter, removes liquid, filtered perilla leaf is dried to 110g.
In remaining perilla leaf be added 0.8%HCl-60% ethyl alcohol 150g, stand 1h, filtering, remove slag, 130 DEG C,
4h is distilled under 0.25Mpa, obtains the active constituent B of 3g.
By 1:1.25 mixing A, B, perilla leaf extract is made.
The macroreticular resin uses following methods to be made: using styrene, divinylbenzene, methacrylate as raw material,
In 0.5% gelatin solution, dimethylbenzene is added and is polymerized.The mass ratio of each component is styrene: divinylbenzene: metering system
Acid esters: gelatin solution: dimethylbenzene=1:0.7:1.2:20:0.8.
Perilla leaf extract made of the present invention includes volatile oil, phenolic acid compound, pigment glycosides compound etc., wherein
Perillaldehyde and perillene recovery rate are about 0.5% to 0.7%, i.e., the perilla leaf of 100g can obtain 5.4g perilla leaf of the invention and extract
Object, perillaldehyde and perillene account for about 0.6g or so.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Within mind and principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (6)
1. a kind of extracting method of perilla leaf extract, which comprises the following steps:
The perilla leaf for taking 100g mass after impregnating 10h in the solution of 1L, is cleaned, is dry；
It crushes, adds 1%HCl-70% ethyl alcohol 160g, stand 1h and extract 100g's after 25KHz ultrasonication 40min
Supernatant liquid extracts 4g active components A after macroporous resin purification；
Remaining perilla leaf is collected, 120g is dried to, 60% ethanol solution 200g, microwave power 200W is added, extracts 20s, is filtered,
Liquid is removed, filtered perilla leaf is dried to 110g；
0.8%HCl-60% ethyl alcohol 150g is added in remaining perilla leaf, stands 1h, filtering is removed slag, at 130 DEG C, 0.25Mpa
4h is distilled, the active constituent B of 3g is obtained,
The solute of the solution consists of the following compositions: the NH of NaCl, 2mmol of HCl, 8mmol of 13mmol4H2PO4、
The H of 1.5mmol3BO3, citric acid 0.6mmol,
By 1:1.25 mixing A, B, perilla leaf extract is made.
2. the extracting method according to claim 1, which is characterized in that perilla leaf is ground into diameter in 5mm or less.
3. the extracting method according to claim 1, which is characterized in that ultrasonic power is 150W to 400W.
4. according to claim 1 to extracting method described in 3 any one, which is characterized in that the macroreticular resin use with
Lower section method is made: using styrene, divinylbenzene, methacrylate as raw material, in 0.5% gelatin solution, diformazan is added
Benzene is polymerized.
5. the extracting method according to claim 4, which is characterized in that the mass ratio of each component is styrene: divinyl
Benzene: methacrylate: gelatin solution: dimethylbenzene=1:0.7:1.2:20:0.8.
6. a kind of perilla leaf extract that the extracting method by claim 1 obtains.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108095030B (en) * | 2017-12-14 | 2021-07-09 | 深圳市千味生物科技有限公司 | Method for extracting natural food taste regulator from perilla leaves |
| CN108277493A (en) * | 2018-04-03 | 2018-07-13 | 西北工业大学 | A kind of cleaning solution and method using perilla leaf extract as corrosion inhibiter |
| CN113082012A (en) * | 2021-04-26 | 2021-07-09 | 北京亿药科技有限公司 | Application of perillene in preparation of medicine for preventing and/or treating hyperuricemia |
| CN113372400A (en) * | 2021-06-09 | 2021-09-10 | 贵州侗乡生态农业科技发展有限公司 | Method for extracting anthocyanin from perilla leaves |
Citations (2)
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| JP4986516B2 (en) * | 2006-06-30 | 2012-07-25 | 京セラ株式会社 | Deposited film forming apparatus and deposited film forming method |
| CN102964866A (en) * | 2012-11-20 | 2013-03-13 | 中北大学 | Method for extracting perilla color from perilla leaves |
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| JPS5221571B2 (en) * | 1972-12-18 | 1977-06-11 |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4986516B2 (en) * | 2006-06-30 | 2012-07-25 | 京セラ株式会社 | Deposited film forming apparatus and deposited film forming method |
| CN102964866A (en) * | 2012-11-20 | 2013-03-13 | 中北大学 | Method for extracting perilla color from perilla leaves |
Non-Patent Citations (2)
| Title |
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| 响应面法优化微波提取紫苏叶多酚物质;王根女等;《中国食品学报》;20091031;第9卷(第5期);第87-92页，尤其是第87页第1.3.2节、第88页2.1.1-2.1.3节 |
| 紫苏精油功能和稳定性研究进展;权美平;《中国调味品》;20140331;第39卷(第3期);第125-128页，尤其是第125页左栏第1段第7-10行 |
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