CN104069172A - Perilla leaf extract and extraction method thereof - Google Patents
Perilla leaf extract and extraction method thereof Download PDFInfo
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- CN104069172A CN104069172A CN201410313498.7A CN201410313498A CN104069172A CN 104069172 A CN104069172 A CN 104069172A CN 201410313498 A CN201410313498 A CN 201410313498A CN 104069172 A CN104069172 A CN 104069172A
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- folium perillae
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Abstract
The invention discloses a perilla leaf extract and an extraction method thereof. The perilla leaf extract comprises 1 part of active ingredient A extracted first and 1.25 parts of active ingredient B extracted for the second time, in parts by weight. After the ingredient A is extracted, polyphenols and anthocyanin in the perilla leaves are removed by use of a microwave method. The ratio of the ingredients of the perilla leaf extract is close to that of oils of the perilla leaves; especially, the ratio of perillaldehyde and the ratio of perillene are relatively high.
Description
Technical field
The present invention relates to a kind of Folium perillae extract and extracting method thereof, it belongs to the chemical extraction technique of plant.
Background technology
The main component of Folium perillae extract has perillaldehyde, pinene He limonene etc., can be used for allocating cosmetics, toothpaste and food flavor.01107031.5 relates to the method for extracting Folium perillae extract from Fructus Perillae, comprise that screening roguing, raw material dewaters, squeeze, filter, come unstuck, deacidification, de-soap, cleaning and disinfecting, thick oily dehydration, sterilization, the step such as cooling, come unstuck, deacidification, take off soap, each operation of dewatering is all carried out between room temperature 40-60 ℃.Prior art also provides some from Folium Perillae, to extract the method for Folium perillae extract.< < response surface method is optimized microwave extraction Folium Perillae polyphenol substance > > (Wang Gennv) and is disclosed the method for extracting polyphenol substance from Folium Perillae.The extraction and analysis of < < Folium Perillae anthocyanin and the research > > (Cai Ningchen) of function thereof relate to the method for extracting anthocyanin from Folium Perillae.Single method extracts Folium Perillae and more or less damages another part composition, and the Folium perillae extract composition extracting is single, can not reduce the ratio of each composition of Folium perillae extract.Be necessary to propose new extracting method, guarantee that abundant extraction, particularly perillaldehyde and the perillene of Folium Perillae volatile oil fully extracts.
Summary of the invention
The present invention proposes a kind of Folium perillae extract and extracting method thereof, can guarantee that volatility oils, particularly perillaldehyde and perillene fully extract.
For solving the problems of the technologies described above, the present invention has constructed following technical proposals: percentage by weight is 0.4-0.5%
An extracting method for Folium perillae extract, is characterized in that, comprises the following steps:
Get the Folium Perillae of 100g quality, in the solution of 1L, soak after 10h, clean, be dried;
Pulverize, add 1%HCl-70% ethanol 160g, standing 1h, after 25KHz ultrasonic Treatment 40min, extracts the supernatant liquid of 100g, after purification by macroporous resin, extracts 4g active components A;
Collect residue Folium Perillae, be dried to 120g, add 60% alcoholic solution 200g, microwave power 200W, extracts 20s, filters, and removes liquid, and the Folium Perillae after filtering is dried to 110g;
In residue, add 0.8%HCl-60% ethanol 150g in Folium Perillae, standing 1h, filters, and removes slag, and under 130 ℃, 0.25Mpa, distills 4h, obtains the active component B of 3g,
Press 1:1.25 mixing A, B, make Folium perillae extract.
In the extracting method of Folium perillae extract of the present invention, the solute of described solution consists of the following composition: the NH of the HCl of 13mmol, the NaCl of 8mmol, 2mmol
4h
2pO
4, 1.5mmol H
3bO
3, citric acid 0.6mmol.After soaking, be convenient to post-processed in solution.
In the extracting method of Folium perillae extract of the present invention, Folium Perillae is ground into diameter below 5mm.
In the extracting method of Folium perillae extract of the present invention, ultrasonic power is 150W to 400W.
In the extracting method of Folium perillae extract of the present invention, described macroporous resin adopts following methods to make: take styrene, divinylbenzene, methacrylate is raw material, in 0.5% gelatin solution, adds dimethylbenzene to be polymerized.
In the extracting method of Folium perillae extract of the present invention, the mass ratio of each component is styrene: divinylbenzene: methacrylate: gelatin solution: dimethylbenzene=1:0.7:1.2:20:0.8.
A Folium perillae extract, is characterized in that, by mass percentage, and the active components A that comprises 1 part, the active component B of 1.25 parts.
Each composition that the present invention makes Folium perillae extract approaches the ratio, particularly perillaldehyde of the oils of Folium Perillae and the ratio of perillene is higher.Polyphenol substance and pigment glycoside material content in Folium Perillae are higher, and withdrawal ratio is also higher, and the present invention, after extracting A, removes polyphenol, the pigment glycosides in Folium Perillae through microwave method, can reduce the ratio of polyphenol, pigment glycoside material.
The specific embodiment
As described in description, the present invention is less to test parameters limited range, below only provides an embodiment.The item not describing in detail in embodiment is referring to the common process in Chemistry for Chinese Traditional Medicine field.
The method of extracting Folium perillae extract from Folium Perillae, comprises the following steps:
Get the Folium Perillae of 100g quality, in the solution of 1L, soak after 10h, clean, be dried, the solute of described solution consists of the following composition: the NH of the HCl of 13mmol, the NaCl of 8mmol, 2mmol
4h
2pO
4, 1.5mmol H
3bO
3, citric acid 0.6mmol.
Be ground into diameter below 5mm, add 1%HCl-70% ethanol 160g, standing 1h, after the ultrasonic Treatment 40min of 25KHz, 300W, extracts the supernatant liquid of 100g, after purification by macroporous resin, extracts 4g active components A.
Collect residue Folium Perillae, be dried to 120g, add 60% alcoholic solution 200g, microwave power 200W, extracts 20s, filters, and removes liquid, and the Folium Perillae after filtering is dried to 110g.
In residue, add 0.8%HCl-60% ethanol 150g in Folium Perillae, standing 1h, filters, and removes slag, and under 130 ℃, 0.25Mpa, distills 4h, obtains the active component B of 3g.
Press 1:1.25 mixing A, B, make Folium perillae extract.
Described macroporous resin adopts following methods to make: take styrene, divinylbenzene, methacrylate is raw material, in 0.5% gelatin solution, adds dimethylbenzene to be polymerized.The mass ratio of each component is styrene: divinylbenzene: methacrylate: gelatin solution: dimethylbenzene=1:0.7:1.2:20:0.8.
The Folium perillae extract that the present invention makes comprises volatile oil, phenolic acid compound, pigment glycosides compound etc., wherein perillaldehyde and perillene extraction ratio are about 0.5% to 0.7%, the Folium Perillae that is 100g can obtain 5.4g Folium perillae extract of the present invention, and perillaldehyde and perillene account for 0.6g left and right.
The foregoing is only preferred embodiment of the present invention, in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (7)
1. an extracting method for Folium perillae extract, is characterized in that, comprises the following steps:
Get the Folium Perillae of 100g quality, in the solution of 1L, soak after 10h, clean, be dried;
Pulverize, add 1%HCl-70% ethanol 160g, standing 1h, after 25KHz ultrasonic Treatment 40min, extracts the supernatant liquid of 100g, after purification by macroporous resin, extracts 4g active components A;
Collect residue Folium Perillae, be dried to 120g, add 60% alcoholic solution 200g, microwave power 200W, extracts 20s, filters, and removes liquid, and the Folium Perillae after filtering is dried to 110g;
In residue, add 0.8%HCl-60% ethanol 150g in Folium Perillae, standing 1h, filters, and removes slag, and under 130 ℃, 0.25Mpa, distills 4h, obtains the active component B of 3g,
Press 1:1.25 mixing A, B, make Folium perillae extract.
2. extracting method according to claim 1, is characterized in that, Folium Perillae is ground into diameter below 5mm.
3. extracting method according to claim 1, is characterized in that, ultrasonic power is 150W to 400W.
4. extracting method according to claim 1, the solute of described solution consists of the following composition: the NH of the HCl of 13mmol, the NaCl of 8mmol, 2mmol
4h
2pO
4, 1.5mmol H
3bO
3, citric acid 0.6mmol.
5. according to the extracting method described in claims 1 to 3 any one, it is characterized in that, described macroporous resin adopts following methods to make: take styrene, divinylbenzene, methacrylate is raw material, in 0.5% gelatin solution, adds dimethylbenzene to be polymerized.
6. extracting method according to claim 5, is characterized in that, the mass ratio of each component is styrene: divinylbenzene: methacrylate: gelatin solution: dimethylbenzene=1:0.7:1.2:20:0.8.
7. a Folium perillae extract, is characterized in that, by mass percentage, and the active components A that comprises 1 part, the active component B of 1.25 parts.
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| CN201410313498.7A CN104069172B (en) | 2014-07-03 | 2014-07-03 | Perilla leaf extract and its extracting method |
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| CN201410313498.7A CN104069172B (en) | 2014-07-03 | 2014-07-03 | Perilla leaf extract and its extracting method |
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| CN104069172B CN104069172B (en) | 2019-01-18 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108095030A (en) * | 2017-12-14 | 2018-06-01 | 成都新柯力化工科技有限公司 | A kind of method that wholefood mouth feel modifying agents are extracted from perilla leaf |
| CN108277493A (en) * | 2018-04-03 | 2018-07-13 | 西北工业大学 | A kind of cleaning solution and method using perilla leaf extract as corrosion inhibiter |
| CN113082012A (en) * | 2021-04-26 | 2021-07-09 | 北京亿药科技有限公司 | Application of perillene in preparation of medicine for preventing and/or treating hyperuricemia |
| CN113372400A (en) * | 2021-06-09 | 2021-09-10 | 贵州侗乡生态农业科技发展有限公司 | Method for extracting anthocyanin from perilla leaves |
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| JPS4986516A (en) * | 1972-12-18 | 1974-08-19 | ||
| CN102964866A (en) * | 2012-11-20 | 2013-03-13 | 中北大学 | Method for extracting perilla color from perilla leaves |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| JP4986516B2 (en) * | 2006-06-30 | 2012-07-25 | 京セラ株式会社 | Deposited film forming apparatus and deposited film forming method |
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| JPS4986516A (en) * | 1972-12-18 | 1974-08-19 | ||
| CN102964866A (en) * | 2012-11-20 | 2013-03-13 | 中北大学 | Method for extracting perilla color from perilla leaves |
Non-Patent Citations (4)
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| 《四川省经济动植物资源开发》编辑组: "《四川省经济动植物资源开发》", 31 May 1988, 四川省社会科学院出版社 * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108095030A (en) * | 2017-12-14 | 2018-06-01 | 成都新柯力化工科技有限公司 | A kind of method that wholefood mouth feel modifying agents are extracted from perilla leaf |
| CN108277493A (en) * | 2018-04-03 | 2018-07-13 | 西北工业大学 | A kind of cleaning solution and method using perilla leaf extract as corrosion inhibiter |
| CN113082012A (en) * | 2021-04-26 | 2021-07-09 | 北京亿药科技有限公司 | Application of perillene in preparation of medicine for preventing and/or treating hyperuricemia |
| CN113372400A (en) * | 2021-06-09 | 2021-09-10 | 贵州侗乡生态农业科技发展有限公司 | Method for extracting anthocyanin from perilla leaves |
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| CN104069172B (en) | 2019-01-18 |
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