CN103820518A - Method for producing protein peptide and plasmin through fermentation of oreochromis niloticus skins and scales - Google Patents
Method for producing protein peptide and plasmin through fermentation of oreochromis niloticus skins and scales Download PDFInfo
- Publication number
- CN103820518A CN103820518A CN201410092219.9A CN201410092219A CN103820518A CN 103820518 A CN103820518 A CN 103820518A CN 201410092219 A CN201410092219 A CN 201410092219A CN 103820518 A CN103820518 A CN 103820518A
- Authority
- CN
- China
- Prior art keywords
- plasmin
- protein peptide
- skin
- fermentation
- fish
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention discloses a method for producing protein peptide and plasmin through fermentation of oreochromis niloticus skins and scales. The method comprises the steps as follows: the oreochromis niloticus skins and scales are cleaned and drained, soaked in hydrochloric acid of 0.4-0.6 mol/L for 20-60 min for decalcification and soaked in sodium hydroxide of 0.1-0.6 mol/L for 30-60 min for degreasing; then the skins and scales and water in a solid-to-liquid ratio of (8-12):100 are added into a fermentation tank to be sterilized at 121 DEG C for 30 min; strains are inoculated into the fermentation tank, and fermentation is conducted at 35 DEG C for 24-48 h; fungus bodies and impurities are removed by filtration after fermentation is finished, and a filtrate I is collected; and the filtrate I is subjected to ultrafiltration through an ultrafiltration membrane so as to obtain a filtrate II containing the protein peptide and a trapped fluid containing the plasmin. According to the method, two products, namely, the protein peptide with the antihypertensive activity and the plasmin capable of dissolving thrombus, are produced simultaneously with the fermentation method directly, so that the raw material utilization rate is high, the production cost is significantly reduced, and the method is environment-friendly and free from environmental pollution and has the wide application value.
Description
Technical field
The present invention relates to biological technical field, relate in particular to a kind of method of utilizing Java tilapia skin fish scale fermentation production of protein peptide and plasmin.
Background technology
Plasmin (Plasmin) refers to the proteolytic ferment of the single-minded fibrin degradation gel of energy, is an important component in fibrinolytic system.Plasmin is mainly by fibrin degradation and Fibrinogen, be hydrolyzed multiple thrombin, make Profibrinolysin change plasmin into, the approach fibrin degradations such as hydrolysis complement make it be degraded to the solvable fragment of small molecules, are easy to decompose and remove from circulation of blood system.Plasmin acts on Fibrinogen and scleroproein, impels tissue plasminogen activator to be discharged by endotheliocyte, and strengthens its activity, tool anti-thrombus function; And can reduce platelet aggregation and blood viscosity, and reduce myocardial consumption of oxygen, improve microcirculation.
Protein peptide is the Molecularly Imprinted Polymer between amino acid and protein, and it is little of being made up of two amino acid, large to being formed by connecting by peptide bond by hundreds of amino acid, has very important biological significance.Modern biological metabolism research is found: the protein of mankind's picked-up is after gastral plurality of enzymes hydrolysis, unlike thinking in the past, only absorbed with amino acid whose form, be more directly to absorb with the form of low peptide, and the absorption rate of dipeptides and tripeptides is faster than the amino acid of same composition.Wherein, some low peptide can not only provide the human body required nutritive substance that grows, and has important physiological function simultaneously.
In prior art, utilize the method preparation of biological enzymolysis more, comprise sodium-chlor removal foreign protein, sodium hydroxide degreasing, adds multiple protein enzyme and carries out enzymolysis and then obtain collagen protein.Utilize enzymolysis process to produce collagen protein and need to add expensive proteolytic enzyme, production cost is higher.At present, still there is no a kind of method production protein peptide simultaneously of microorganism fermentation and method of plasmin of adopting take Java tilapia skin fish scale as raw material.
Summary of the invention
The object of the present invention is to provide a kind of fish skin and scale that utilizes tilapia to produce simultaneously and there is the protein peptide of important biomolecule activity and the method for plasmin for raw material.
The technical solution adopted in the present invention is to provide a kind of method of utilizing Java tilapia skin fish scale fermentation production of protein peptide and plasmin, comprises the following steps:
(1) raw material processing: Java tilapia skin fish scale is cleaned and drained, and the hydrochloric acid soln that is placed in 0.4～0.6mol/L soaks 20～60min decalcification, is then washed to neutrality and drains; The sodium hydroxide solution that is placed in 0.1～0.6mol/L through decalcification fish skin and scale after treatment is soaked to 30～60min degreasing, be then washed to neutrality, blend dry for standby;
(2) sterilizing: the fish skin and scale of handling well in step 1 and water are added in fermentor tank to 121 ℃ of sterilizing 30min in the ratio of solid-to-liquid ratio 8-12:100;
(3) inoculation fermentation: to access in fermentor tank through the subtilis of liquid nutrient medium enlarged culturing in 35 ℃ of condition bottom fermentation 24～48h; The bacterial classification amount of access is 2～6%;
(4) fermentation ends, filters and removes thalline and impurity, collects filtrate I; Described filtrate I obtains the filtrate II that contains protein peptide and the trapped fluid that contains plasmin through ultra-filtration membrane ultrafiltration again;
(5) described in, contain the filtrate II of protein peptide through vacuum concentration, spraying is dried to obtain described protein peptide; The described trapped fluid that contains plasmin obtains described plasmin through lyophilize.
The present invention also aims to provide described protein peptide that the method for Java tilapia skin fish scale fermentation production of protein peptide and plasmin the obtains application in food, healthcare products or makeup that utilizes.
The present invention also aims to provide described protein peptide and the application of plasmin in healthcare products or the medicine of thrombus and antithrombotic that utilizes the method for Java tilapia skin fish scale fermentation production of protein peptide and plasmin to obtain.
Beneficial effect of the present invention is, utilize waste material fish-skin in tilapia process of manufacture and/or fish scale as raw materials for production, the method of employing microorganism fermentation makes simultaneously has the protein peptide of antihypertensive activity and two kinds of products of the plasmin of thrombus activity, and do not need to add expensive proteolytic enzyme and additive, production technique is simple, with low cost.
Accompanying drawing explanation
Fig. 1 is the bacteriolyze circle of urokinase on agarose-fibrin plate, and wherein, 1～5 corresponding enzyme activity is respectively 20,40,60,80,160IU/ml;
Fig. 2 is urokinase typical curve;
Fig. 3 is the high-efficient liquid phase chromatogram of the prepared protein peptide of the embodiment of the present invention;
Fig. 4 is that the ACE of the prepared protein peptide of the embodiment of the present invention suppresses active comparison diagram (7 peaks in corresponding diagram 3).
Embodiment
In order to make technical problem to be solved by this invention, technical scheme and beneficial effect clearer, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
The embodiment of the present invention provides a kind of method of utilizing Java tilapia skin fish scale fermentation production of protein peptide and plasmin, comprises the following steps:
(1) raw material processing: Java tilapia skin fish scale is cleaned and drained, and the hydrochloric acid soln that is placed in 0.4～0.6mol/L soaks 20～60min decalcification, is then washed to neutrality and drains; The sodium hydroxide solution that is placed in 0.1～0.6mol/L through decalcification fish skin and scale after treatment is soaked to 30～60min degreasing, be then washed to neutrality, blend dry for standby;
(2) sterilizing: the fish skin and scale of handling well in step 1 and water are added in fermentor tank to 121 ℃ of sterilizing 30min in the ratio of solid-to-liquid ratio 8-12:100;
(3) inoculation fermentation: to access in fermentor tank through the subtilis of liquid nutrient medium enlarged culturing in 35 ℃ of condition bottom fermentation 24～48h; The bacterial classification amount of access is 2～6%;
(4) fermentation ends, filters and removes thalline and impurity, collects filtrate I; Described filtrate I makes the filtrate II that contains protein peptide and the trapped fluid that contains plasmin through ultra-filtration membrane ultrafiltration again;
(5) described in, contain the filtrate II of protein peptide through vacuum concentration, spraying is dried to obtain described protein peptide; The described trapped fluid that contains plasmin obtains described plasmin through lyophilize.
Particularly, described Java tilapia skin fish scale comprises the mixture of a kind of or two kinds of arbitrary proportions in the fish-skin of tilapia and fish scale.While Java tilapia skin fish scale being carried out to raw material processing in step 1, the solid-liquid mass ratio of fish skin and scale and hydrochloric acid soln is 1:4～8; Through decalcification fish skin and scale sodium hydroxide solution after treatment and solid-liquid mass ratio be 1:4～8.It need be that 5000 daltonian ultra-filtration membranes filter again through molecular weight cut-off that the filtrate I that collection obtains after thalline and impurity is filtered away in fermentation.Ultrafiltration gained filtrate II is through vacuum concentration, and spraying is dried and makes protein peptide powder, the active plasmin powder of ultrafiltration gained trapped fluid obtained by freeze drying.
Further illustrate below in conjunction with design parameter.
Take Java tilapia skin 5kg cleaning and drain, add 0.2mol/L sodium hydroxide 40L, soak 30min degreasing, be washed to neutrality, blend dry for standby.Add fish-skin and water in fermentor tank by solid-liquid mass ratio 8:100,121 ℃, sterilizing in 30 minutes.Inoculum size 2% is inoculated subtilis, and leavening temperature is 35 ℃, ferments 24 hours.Fermentation ends, fermented liquid Plate Filtration is removed thalline and impurity, collects filtered liquid.5000dalton ultra-filtration membrane ultrafiltration for filtered liquid, filtered liquid is protein peptide, trapped fluid is plasmin.Filtered liquid is through vacuum concentration, and spraying is dried to obtain protein peptide powder.Plasmin obtains organized enzyme powder through lyophilize.
The mixture 5kg that takes Java tilapia skin and fish scale cleans and drains, and adding concentration is the hydrochloric acid soln 60L of 0.4mol/L, soaks 30min decalcification, drains after being washed to neutrality; Add again 60 liters, 0.4mol/L sodium hydroxide, soak degreasing in 40 minutes, be washed to neutrality, blend dry for standby.Add fish skin and scale mixture and water in fermentor tank in the ratio of solid-liquid mass ratio 12:100,121 ℃, sterilizing 30 minutes.By 4% inoculum size inoculation subtilis, leavening temperature is 35 ℃, ferments 36 hours.Fermentation ends, fermented liquid Plate Filtration is removed thalline and impurity, collects filtered liquid.5000dalton ultra-filtration membrane ultrafiltration for filtered liquid, filtered liquid is protein peptide, trapped fluid is plasmin.Filtered liquid is through vacuum concentration, and spraying is dried to obtain protein peptide powder.Plasmin obtains organized enzyme powder through lyophilize.
The mixture 5kg that takes Java tilapia skin and fish scale cleans and drains, and adding concentration is the hydrochloric acid soln 60L of 0.6mol/L, soaks 30min decalcification, drains after being washed to neutrality; Add 0.6mol/L sodium hydroxide 80L, degreasing 60 minutes, is washed to neutrality, blends dry for standby.Add fish-skin and water in fermentor tank in solid-to-liquid ratio 10:100 ratio, 121 ℃, sterilizing in 30 minutes.Inoculum size 6% is inoculated subtilis, and leavening temperature is 35 ℃, ferments 48 hours.Fermentation ends, fermented liquid Plate Filtration is removed thalline and impurity, collects filtered liquid.5000dalton ultra-filtration membrane ultrafiltration for filtered liquid, filtered liquid is protein peptide, trapped fluid is plasmin.Filtered liquid is through vacuum concentration, and spraying is dried to obtain protein peptide powder.Plasmin obtains organized enzyme powder through lyophilize.
Experimental example 1 plasmin thrombolysis activity test
The thrombolysis activity of plasmin is measured by national standard agarose-fibrin plate method, get respectively 20,40,60,80, the sample solution of 160IU/mL is added drop-wise on fibrin plate, as shown in Figure 1, measure the area of transparent circle, take the logarithmic value of urokinase unit of activity as X-coordinate, the logarithmic value of transparent circle area is ordinate zou, drawing standard curve, as shown in Figure 2.The thrombolysis activity that represents plasmin according to typical curve with urokinase unit of activity, the calculation formula that records plasmin vigor is:
Fibrinolytic enzyme vigor (IU/mL)=0.843M-84.54;
M in formula---solusphere area (mm
2).
It is 215U/mg that experiment records in the embodiment of the present invention 1～3 the obtained plasmin mean value of living, and effectively thrombus is with a wide range of applications in the healthcare products of thrombus and antithrombotic or medicine.
The antihypertensive activity test of experimental example 2 protein peptide
Suppressing activity methods with ultraviolet spectrophotometer angiotensin converting enzyme-inhibiting peptide (ACE) measures:
S1: get 100 μ l HHL(Hip-His-Leu) solution and 40 μ l culture supernatants mix in 2ml EP pipe, in 37 ℃ of insulation 3min, add 10 μ l ACE solution, in 37 degree insulation reaction 30min, add 250 μ l1mol/L HCl solution termination reactions.Add 1.5ml vinyl acetic monomer, 15s vibration mixes again, and 5000 revs/min of centrifugal 10min draw 1ml vinyl acetic monomer layer with liquid-transfering gun, and dry 1h at 100 ℃, adds 3ml deionized water, at its OD value of the mensuration A of 228nm place.Wherein, reference liquid is 4ml damping fluid.
S2: change 40 μ l cultures in step S1 into 40 μ l deionized waters, other are constant, record OD value B.
S3: change 10 μ l ACE solution in step 1 into 10 μ l deionized waters, other are constant, record OD value C.
S4: calculating ACE inhibiting rate (%)=(B-A)/(B-C) * 100%
The obtained activated protein peptide of the present invention obtains 7 peaks through high performance liquid chromatography chromatographic separation, as shown in Figure 3, number consecutively is 1～7, the ACE inhibiting rate at measured 7 peaks is 18.40～68.40%, wherein the ACE inhibiting rate at the 5th peak is the highest, can reach 68.40%, there is efficient antihypertensive activity, can be used for preparing healthcare products and the medicine of antihypertensive activity.
The inventive method can make protein peptide and the plasmin with important biomolecule activity simultaneously, described protein peptide molecular mass is little, being beneficial to health directly absorbs with the form of low peptide, and absorption rate is faster than the amino acid of same composition, the human body required nutritive substance that grows can be provided, can be used for preparation and have food, healthcare products or the makeup of specific function.Obtained activated protein peptide composition is abundant, containing just like angiotensin converting enzyme-inhibiting peptide (ACE), can be used for preparing healthcare products or the medicine with antihypertensive activity; Obtained activated protein peptide also can be used as the further separating-purifying of raw material and makes other bioactive peptides, as promoted mineral absorption peptide, prevent and treat hepatogenic encephalopathy peptide, absorption peptide easy to digest, antibacterial peptide, morphine sheet peptide, class morphine antagonistic peptide, suppress cholesterol effect peptide, body defense function peptide.
Further, the present invention utilizes waste material fish-skin in tilapia process of manufacture and/or fish scale as raw materials for production, the method of employing microorganism fermentation makes simultaneously has the protein peptide of antihypertensive activity and two kinds of products of the plasmin of thrombus activity, and do not need to add expensive proteolytic enzyme and additive, production technique is simple, with low cost.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (7)
1. a method of utilizing Java tilapia skin fish scale fermentation production of protein peptide and plasmin, is characterized in that, comprises the following steps:
(1) raw material processing: Java tilapia skin fish scale is cleaned and drained, and the hydrochloric acid soln that is placed in 0.4～0.6mol/L soaks 20～60min decalcification, is then washed to neutrality and drains; The sodium hydroxide solution that is placed in 0.1～0.6mol/L through decalcification fish skin and scale after treatment is soaked to 30～60min degreasing, be then washed to neutrality, blend dry for standby;
(2) sterilizing: the fish skin and scale of handling well in step 1 and water are added in fermentor tank to 121 ℃ of sterilizing 30min in the ratio of solid-to-liquid ratio 8～12:100;
(3) inoculation fermentation: to access in fermentor tank through the subtilis of liquid nutrient medium enlarged culturing in 35 ℃ of condition bottom fermentation 24～48h; The bacterial classification amount of access is 2～6%;
(4) fermentation ends, filters and removes thalline and impurity, collects filtrate I; Described filtrate I obtains the filtrate II that contains protein peptide and the trapped fluid that contains plasmin through ultra-filtration membrane ultrafiltration again;
(5) described in, contain the filtrate II of protein peptide through vacuum concentration, spraying is dried to obtain described protein peptide; The described trapped fluid that contains plasmin obtains described plasmin through lyophilize.
2. the method for utilizing Java tilapia skin fish scale fermentation production of protein peptide and plasmin according to claim 1, is characterized in that, described Java tilapia skin fish scale comprises fish-skin and/or the fish scale of tilapia.
3. the method for utilizing Java tilapia skin fish scale fermentation production of protein peptide and plasmin according to claim 1 and 2, is characterized in that, the solid-liquid mass ratio of described fish skin and scale and described hydrochloric acid soln is 1:4～8.
4. the method for utilizing Java tilapia skin fish scale fermentation production of protein peptide and plasmin according to claim 1, is characterized in that, the described solid-liquid mass ratio through decalcification fish skin and scale after treatment and described sodium hydroxide solution is 1:4～8.
5. the method for utilizing Java tilapia skin fish scale fermentation production of protein peptide and plasmin according to claim 1, is characterized in that, the molecular weight cut-off of described ultra-filtration membrane is 5000 dalton.
6. the application of the protein peptide of the method acquisition that utilizes Java tilapia skin fish scale fermentation production of protein peptide and plasmin as described in claim 1～5 any one in food, healthcare products or makeup.
7. the application of the plasmin of the method acquisition that utilizes Java tilapia skin fish scale fermentation production of protein peptide and plasmin as described in claim 1～5 any one in healthcare products or the medicine of thrombus and antithrombotic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410092219.9A CN103820518A (en) | 2014-03-13 | 2014-03-13 | Method for producing protein peptide and plasmin through fermentation of oreochromis niloticus skins and scales |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410092219.9A CN103820518A (en) | 2014-03-13 | 2014-03-13 | Method for producing protein peptide and plasmin through fermentation of oreochromis niloticus skins and scales |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103820518A true CN103820518A (en) | 2014-05-28 |
Family
ID=50755806
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410092219.9A Pending CN103820518A (en) | 2014-03-13 | 2014-03-13 | Method for producing protein peptide and plasmin through fermentation of oreochromis niloticus skins and scales |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103820518A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109350731A (en) * | 2018-12-18 | 2019-02-19 | 汕尾市维明生物科技股份有限公司 | A kind of Rofe fish protein peptide is preparing the application in immune formulation |
| CN110074356A (en) * | 2019-06-04 | 2019-08-02 | 江南大学 | A kind of Java tilapia skin deliming degreasing method |
| CN117683628A (en) * | 2024-02-04 | 2024-03-12 | 德州蓝力生物技术有限公司 | Device and method for extracting protein peptide from tilapia skin and scales |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1451745A (en) * | 2002-04-15 | 2003-10-29 | 黑龙江省科学院应用微生物研究所 | Natto kinase produced using Bacillus subtilis, and producing method and use thereof |
| CN101240313A (en) * | 2008-03-07 | 2008-08-13 | 陈成 | Method for preparing collagen peptide |
-
2014
- 2014-03-13 CN CN201410092219.9A patent/CN103820518A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1451745A (en) * | 2002-04-15 | 2003-10-29 | 黑龙江省科学院应用微生物研究所 | Natto kinase produced using Bacillus subtilis, and producing method and use thereof |
| CN101240313A (en) * | 2008-03-07 | 2008-08-13 | 陈成 | Method for preparing collagen peptide |
Non-Patent Citations (3)
| Title |
|---|
| 章海锋等: "微生物发酵生产纤溶酶研究进展", 《粮油加工》 * |
| 范晓丹等: "产纤溶酶菌种的鉴定及纤溶酶的分离纯化", 《华南理工大学学报（自然科学版）》 * |
| 陈志文等: "枯草芽孢杆菌产纤溶酶发酵条件的研究", 《山西食品工业》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109350731A (en) * | 2018-12-18 | 2019-02-19 | 汕尾市维明生物科技股份有限公司 | A kind of Rofe fish protein peptide is preparing the application in immune formulation |
| CN110074356A (en) * | 2019-06-04 | 2019-08-02 | 江南大学 | A kind of Java tilapia skin deliming degreasing method |
| CN117683628A (en) * | 2024-02-04 | 2024-03-12 | 德州蓝力生物技术有限公司 | Device and method for extracting protein peptide from tilapia skin and scales |
| CN117683628B (en) * | 2024-02-04 | 2024-04-26 | 德州蓝力生物技术有限公司 | Device and method for extracting protein peptide from tilapia skin and scales |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102318724B (en) | Production method of acid soybean peptide protein | |
| CN101297821B (en) | Phellinus linteus mycelia active glucoprotein and use thereof and preparation | |
| CN102028091B (en) | Method for preparing low-molecular fish peptide by bacillus natto fermentation method | |
| CN101899484A (en) | Preparation method of genipin | |
| CN102242165A (en) | Method for producing high molecular weight sodium hyaluronate through fermentation and culture medium utilized by same | |
| CN105255974A (en) | Method for preparing antioxidant active peptide by means of fermenting tilapia skin through bacillus subtilis | |
| CN103820518A (en) | Method for producing protein peptide and plasmin through fermentation of oreochromis niloticus skins and scales | |
| CN110495611A (en) | A kind of technique improving sea cucumber nutritional health effect | |
| CN109251954A (en) | A kind of production method of sea cucumber polypeptide | |
| CN203546025U (en) | Enclosed simultaneous saccharification lactic acid production line | |
| CN103898013B (en) | The preparation of bacterium and kappa-carrageenan enzyme is revolved in one strain sea | |
| CN106134771A (en) | A kind of Se-rich xianggu collagen cultivation matrix | |
| CN110495579A (en) | A kind of technique improving globefish skin nutritive value | |
| CN107198673A (en) | Marine organisms care compositions and its production method | |
| CN102382808B (en) | Preparation method of renninum | |
| CN105255972A (en) | Method for preparing antioxidant active peptide by means of fermenting tilapia skin through aspergillus oryzae | |
| CN103642885A (en) | Method for producing wheat biological active peptide by fermentation | |
| CN102676482A (en) | Method for producing alkali protease from highland bacillus | |
| CN102392062A (en) | Method for preparing bacterial cellulose by using decayed fruits as raw materials | |
| CN107412077A (en) | It is a kind of it is sun-proof repair skin olive growing leaves it is ultraviolet stress submerged fermentation extract preparation method | |
| CN107441479A (en) | Marine active peptide/chitin burn ointment for treating scald and preparation method thereof | |
| CN105255982A (en) | Method for preparing antioxidant active peptide by means of fermenting tilapia skin through aspergillus oryzae | |
| CN103898012A (en) | Thalassospira sp. strain and method for preparing agarase | |
| CN106316578A (en) | Nutrient-adsorption type shii-take collagen culture medium | |
| CN103923853B (en) | One strain series bacillus and the preparation method for kappa-carrageenan enzyme thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140528 |