CN106316578A - Nutrient-adsorption type shii-take collagen culture medium - Google Patents
Nutrient-adsorption type shii-take collagen culture medium Download PDFInfo
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- CN106316578A CN106316578A CN201610645896.8A CN201610645896A CN106316578A CN 106316578 A CN106316578 A CN 106316578A CN 201610645896 A CN201610645896 A CN 201610645896A CN 106316578 A CN106316578 A CN 106316578A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
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Abstract
The invention discloses a nutrient-adsorption type shii-take collagen culture medium. The nutrient-adsorption type shii-take collagen culture medium is prepared from, by weight, 3-5 parts of sodium carboxymethyl cellulose, 2-3 parts of sodium polyacrylate, 38-42 parts of brewer's grains, 14-16 parts of tangerine seed powder, 4-6 parts of chitosan, 2-3 parts of copper sulfate powder, 3-4 parts of potassium phosphate, 6-8 parts of zeolite powder, 38-42 parts of pigskin waste materials, 1-2 parts of sodium chloride powder, an appropriate quantity of acetic acid solution with pH 2.5 and sodium hydroxide solution and an appropriate amount of water. sodium carboxymethyl cellulose, sodium polyacrylate and other high-water absorption materials are adopted for effectively absorbing a lot of liquefied nutrients in a nutrient solution and fixing the liquefied nutrients in the culture medium, the nutrients will be not lost along with changes of environment, and it is ensured that during growth, the nutrients of shii-take are sufficiently provided.
Description
Technical field
The present invention relates to fungus growing technique field, particularly relate to a kind of nutrition absorbent-type Lentinus Edodes collagen cultivation matrix.
Background technology
Lentinus Edodes is commonly called as China mushroom, is that mycophagy medical material is cultivated in a kind of famous using, has long cultivation in China
Historical tradition, the nutritive value abundant because of it and medical value and deeply liked by consumer, become the second adult in the world
The edible fungi of work cultivation, due to the fast development of industry, the raw material of Lentinus Edodes substituting stuff cultivation traditional matrices is the most exhausted, needs actively
Explore the approach substituting substrate.On the other hand, along with the raising of people's living standard, it is desirable to Lentinus Edodes nutritive value and medicinal
It is worth and also can be improved, so needing to provide the mushroom cultivation substrate of a kind of high-quality.
Collagen protein as a kind of biomacromolecule, have natural hypotoxicity, hypoimmunity, bootable mycelial growth with
And the preferable advantage such as biocompatibility, its extracting method is simple simultaneously, and raw material sources extensively and cheap, are discarded by slaughterhouse
Corii Sus domestica can carry out scale extraction, it has a very high potential as the New-type matrix material of cultivating champignon, but Non-crosslinked
The collagen protein of moditied processing often has that degradation rate is too fast, deformation temperature is low, easily shrink deformation and mechanical performance
The shortcomings such as deficiency, need by the cross-linking modified mechanical performance improving collagen protein and anti-degradation capability.
At present, the method that collagen-stabilizedization processes mainly is cross-linked by method physically or chemically.Wherein,
Physical method has vacuum dehydrothermal, ultraviolet and C ray crosslinking etc., and the advantage of these methods is not introduce toxic chemical substance,
Can keep the biocompatibility that collagen is good, but individually use physical crosslinking tend not to obtain homogeneous, preferably crosslinking strong
Degree, therefore Chemical Crosslinking Methods is more widely applied.
Common chemical cross-linking agent has aldehydes, Carbodiimides, bis-epoxy class material and the different hydrochlorate of cyanogen etc..Wherein,
Glutaraldehyde is the cross-linking agent that a class is widely used in collagen base biological material, can reduce the antigenicity of collagen, but glutaraldehyde is handed over
There is the reduction collagenous biological compatibility and be easily caused the potential danger such as collagen calcification in connection agent.Research shows, even if glutaraldehyde is residual
When staying mass concentration as little as 310mg/L the most toxic, its functional group not reacted or collagen drop under the effect of enzyme
These functional groups discharged during solution all may cause cell-cytotoxic reaction, works the mischief to cultivating bacterium.And 1-(3-dimethylamino
Propyl group)-3-ethyl-carbodiimide hydrochloride/N-hydroxy-succinamide cross-linking agent has spy nontoxic, that biocompatibility is good
Point, applies and can show the most excellent performance in the modification of collagen protein.
The application for a patent for invention of Publication No. 102617213A discloses a kind of utilization compression straw and prepares cultivating champignon base
The method of matter, this culture matrix uses weight ratio to be the thick wood flour of 25-35%, the thin wood flour of 15-25%, the pressure being impregnated with of 25-35%
Contracting stalk particle, the wheat bran of 10-15%, the Gypsum Fibrosum of 1.0-1.5% are mixed with and form, and this invention culture matrix content of lignin is relatively
The utilization of height, beneficially mushroom mycelium, uses agricultural production waste material as cultivation matrix raw material simultaneously, improves environment and saving
Cost, but the cultivation matrix of this disclosure of the invention uses raw material the most single, easily causes nutrient unbalanced, needs extra nutritional thing
The interpolation of matter, simultaneously the highest with the biocompatibility of Lentinus Edodes, the extension with mycelia is improved without promoting for mushroom growth microenvironment
Enter effect, without the raising of notable surcharge, limit the scale application of invention substrate.
Summary of the invention
The object of the invention is contemplated to make up the defect of prior art, it is provided that a kind of nutrition absorbent-type Lentinus Edodes collagen cultivation base
Matter.
The present invention is achieved by the following technical solutions:
A kind of nutrition absorbent-type Lentinus Edodes collagen cultivation matrix, is prepared by the raw materials in: sodium carboxymethyl cellulose 3-5,
Sodium polyacrylate 2-3, distiller's grains of beer 38-42, Semen Citri Reticulatae powder 14-16, oligo-chitosan 4-6, copper sulphate powder 2-3, potassium phosphate 3-4,
Zeolite powder 6-8, Corii Sus domestica waste material 38-42, sodium chloride powder 1-2, pepsin preparation 1-2,1-(3-dimethylamino-propyl)-3-second
Base carbodiimide hydrochloride 1.4-1.6, N-hydroxy-succinamide 0.3-0.4,1% sodium chloride solution are appropriate, pH2.5 acetic acid is molten
Liquid is appropriate, sodium hydroxide solution is appropriate, water is appropriate.
Specifically comprising the following steps that of described nutrition absorbent-type Lentinus Edodes collagen cultivation matrix preparation method
(1) adding water after distiller's grains of beer, Semen Citri Reticulatae powder being sufficiently mixed loose to material humidity, retting is fermented 24-26 days, by fermentation material pressure
Squeeze and filter, obtain fermentation liquid and fermentation siccative, then fermentation liquid is individually taken out, collect bacterium mud precipitation the most afterwards and upper strata is sent out
Ferment clear liquid, makes lyophilized powder by bacterium mud lyophilization, i.e. obtains fermentation siccative, fermentation clear liquid and lyophilized powder standby；
(2) in step 1 gained fermentation clear liquid, add oligo-chitosan, copper sulphate powder, be heated to 82-85 DEG C, be stirred continuously
Chelating 25-30 minute, be cooled to room temperature afterwards, add potassium phosphate, sodium polyacrylate, stirring mixing, to uniformly, obtains nutritional solution
Standby；
(3) Corii Sus domestica waste material unhairing afterwash is shredded, join in 1% sodium chloride solution by the solid-to-liquid ratio of 1:5-6g/mL, soak
Clean with clear water after 5-6 hour, then it is molten that by the solid-to-liquid ratio of 1:15-18g/mL, gained skin bit is joined the acetic acid that pH value is 2.5
In liquid, and soak 8-10 hour under condition of ice bath, after completing, skin bit is smashed, now add the above-mentioned acetic acid of same volume
Solution, and add pepsin preparation, enzymolysis 12-15 hour, the most per hour stirring 5-7 minute, enzymolysis uses bilayer after terminating
Filtered through gauze, collects filtrate, is added thereto to sodium hydroxide solution regulation PH to 7-8, adds sodium chloride powder, stand 10-
12 hours, afterwards that solution is centrifugal 15-18 minute with 5000-5500 rev/min, collect precipitation, after drying, obtain collagen protein powder
Standby；
(4) by step 1 gained fermentation siccative, lyophilized powder, step 2 gained nutritional solution, step 3 gained collagen protein powder and carboxylic
After sodium carboxymethylcellulose pyce, zeolite powder all mix and be sufficiently stirred for, it is placed under 2-4 DEG C of environment, now adds 1-(3-dimethylamino
Propyl group)-3-ethyl-carbodiimide hydrochloride and N-hydroxy-succinamide, cross-link 10-12 hour, be warmed to room temperature after completing, i.e.
Obtain mushroom cultivation substrate of the present invention.
The invention have the advantage that
Substrate of the present invention by extracting the glue by having reduced immunogenicity, biodegradability to collagen protein in Corii Sus domestica waste material
Former albumen is applied in the preparation of cultivation matrix, use simultaneously 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride/
N-hydroxy-succinamide carries out crosslinking Treatment as cross-linking agent to collagen, makes denaturation temperature and the resistance to enzymolysis ability of collagen protein
Improving, water absorption rate and swelling ratio reduce, and improve its heat stability and structural stability, can not only provide certain propping up for Lentinus Edodes
Support and growth interface, the porous network structure that the interlaced arrangement of collagenous fiber bundle within collagen protein is formed simultaneously, also have
It is beneficial to beneficial microorganism carry and nutritional labeling fixing, the most beneficially the adhesion of mushroom mycelium and moving into, for Lentinus Edodes
Growth provide environment suitable, that nutrient is sufficient.
The mushroom cultivation substrate of the present invention have employed the high absorbency material such as sodium carboxymethyl cellulose, sodium polyacrylate, energy
A large amount of liquid nutrients in active adsorption nutritional solution, and being fixed in culture matrix, make the nutritional labeling will not be easily with environment
Change and run off, it is ensured that in during mushroom growth, nutrient is sufficiently fed.
Detailed description of the invention
A kind of nutrition absorbent-type Lentinus Edodes collagen cultivation matrix, is made up of the raw material of following weight portion (kg): carboxymethyl cellulose
Element sodium 3, sodium polyacrylate 2, distiller's grains of beer 38, Semen Citri Reticulatae powder 14, oligo-chitosan 4, copper sulphate powder 2, potassium phosphate 3, zeolite powder 6,
Corii Sus domestica waste material 38, sodium chloride powder 1, pepsin preparation 1,1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride
1.4, N-hydroxy-succinamide 0.3,1% sodium chloride solution is appropriate, pH2.5 acetum is appropriate, sodium hydroxide solution appropriate,
Water is appropriate.
Specifically comprising the following steps that of described nutrition absorbent-type Lentinus Edodes collagen cultivation matrix preparation method
(1) adding water after distiller's grains of beer, Semen Citri Reticulatae powder being sufficiently mixed loose to material humidity, retting is fermented 24 days, is squeezed by fermentation material
And filter, obtain fermentation liquid and fermentation siccative, then fermentation liquid is individually taken out, collect bacterium mud precipitation and high fermentation the most afterwards
Clear liquid, makes lyophilized powder by bacterium mud lyophilization, i.e. obtains fermentation siccative, fermentation clear liquid and lyophilized powder standby；
(2) in step 1 gained fermentation clear liquid, add oligo-chitosan, copper sulphate powder, be heated to 82 DEG C, be stirred continuously chelating
25 minutes, being cooled to room temperature afterwards, add potassium phosphate, sodium polyacrylate, stirring mixing, to uniformly, obtains nutritional solution standby；
(3) Corii Sus domestica waste material unhairing afterwash is shredded, join in 1% sodium chloride solution by the solid-to-liquid ratio of 1:5g/mL, soak 5 little
Shi Houyong clear water is cleaned, then is joined in the acetum that pH value is 2.5 by the solid-to-liquid ratio of 1:15g/mL by gained skin bit, and
Soak 8 hours under condition of ice bath, after completing, skin bit is smashed, now add the above-mentioned acetum of same volume, and add
Pepsin preparation, enzymolysis 12 hours, the most per hour stirring 5 minutes, enzymolysis filters with double gauze after terminating, collects filter
Liquid, be added thereto to sodium hydroxide solution regulation PH to 7, add sodium chloride powder, stand 10 hours, afterwards by solution with
5000 revs/min are centrifuged 15 minutes, collect precipitation, obtain collagen protein powder standby after drying；
(4) by step 1 gained fermentation siccative, lyophilized powder, step 2 gained nutritional solution, step 3 gained collagen protein powder and carboxylic
After sodium carboxymethylcellulose pyce, zeolite powder all mix and be sufficiently stirred for, it is placed under 2 DEG C of environment, now adds 1-(3-dimethylamino third
Base)-3-ethyl-carbodiimide hydrochloride and N-hydroxy-succinamide, cross-link 10 hours, be warmed to room temperature after completing, i.e. get Ben Fa
Bright mushroom cultivation substrate.
In order to further illustrate the using value of the present invention, inventor uses substrate of the present invention and commercially available generic media respectively
Carrying out the cultivating and growing of Lentinus Edodes, in addition to using substrate difference, other management methods are the most identical, and data measured is substrate of the present invention institute
The more conventional substrate of biological transformation ratio of Lentinus Edodes improves 4.7%, and the output increased of finished product mushroom 14.8%, the color of mushroom simultaneously
Snow-white, good mushroom type relatively, illustrates that Lentinus Edodes substrate of the present invention has preferable using effect.
Claims (2)
1. a nutrition absorbent-type Lentinus Edodes collagen cultivation matrix, it is characterised in that be prepared by the raw materials in: carboxymethyl
Sodium cellulosate 3-5, sodium polyacrylate 2-3, distiller's grains of beer 38-42, Semen Citri Reticulatae powder 14-16, oligo-chitosan 4-6, copper sulphate powder 2-
3, potassium phosphate 3-4, zeolite powder 6-8, Corii Sus domestica waste material 38-42, sodium chloride powder 1-2, pepsin preparation 1-2,1-(3-diformazan ammonia
Base propyl group)-3-ethyl-carbodiimide hydrochloride 1.4-1.6, N-hydroxy-succinamide 0.3-0.4,1% sodium chloride solution be appropriate,
PH2.5 acetum is appropriate, sodium hydroxide solution is appropriate, water is appropriate.
2. according to the nutrition absorbent-type Lentinus Edodes collagen cultivation matrix described in claims 1, it is characterised in that the tool of preparation method
Body step is as follows:
(1) adding water after distiller's grains of beer, Semen Citri Reticulatae powder being sufficiently mixed loose to material humidity, retting is fermented 24-26 days, by fermentation material
Squeeze and filter, obtain fermentation liquid and fermentation siccative, then fermentation liquid is individually taken out, collect bacterium mud precipitation and upper strata the most afterwards
Fermentation clear liquid, makes lyophilized powder by bacterium mud lyophilization, i.e. obtains fermentation siccative, fermentation clear liquid and lyophilized powder standby；
(2) in step 1 gained fermentation clear liquid, add oligo-chitosan, copper sulphate powder, be heated to 82-85 DEG C, be stirred continuously
Chelating 25-30 minute, be cooled to room temperature afterwards, add potassium phosphate, sodium polyacrylate, stirring mixing, to uniformly, obtains nutritional solution
Standby；
(3) Corii Sus domestica waste material unhairing afterwash is shredded, join in 1% sodium chloride solution by the solid-to-liquid ratio of 1:5-6g/mL, soak
Clean with clear water after 5-6 hour, then it is molten that by the solid-to-liquid ratio of 1:15-18g/mL, gained skin bit is joined the acetic acid that pH value is 2.5
In liquid, and soak 8-10 hour under condition of ice bath, after completing, skin bit is smashed, now add the above-mentioned acetic acid of same volume
Solution, and add pepsin preparation, enzymolysis 12-15 hour, the most per hour stirring 5-7 minute, enzymolysis uses bilayer after terminating
Filtered through gauze, collects filtrate, is added thereto to sodium hydroxide solution regulation PH to 7-8, adds sodium chloride powder, stand 10-
12 hours, afterwards that solution is centrifugal 15-18 minute with 5000-5500 rev/min, collect precipitation, after drying, obtain collagen protein powder
Standby；
(4) by step 1 gained fermentation siccative, lyophilized powder, step 2 gained nutritional solution, step 3 gained collagen protein powder and
After sodium carboxymethyl cellulose, zeolite powder all mix and be sufficiently stirred for, it is placed under 2-4 DEG C of environment, now adds 1-(3-diformazan ammonia
Base propyl group)-3-ethyl-carbodiimide hydrochloride and N-hydroxy-succinamide, cross-link 10-12 hour, be warmed to room temperature after completing,
Obtain mushroom cultivation substrate of the present invention.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610645896.8A CN106316578A (en) | 2016-08-09 | 2016-08-09 | Nutrient-adsorption type shii-take collagen culture medium |
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| CN201610645896.8A CN106316578A (en) | 2016-08-09 | 2016-08-09 | Nutrient-adsorption type shii-take collagen culture medium |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107903103A (en) * | 2017-10-30 | 2018-04-13 | 穆世鹏 | A kind of culture substrate for promoting edible fungi growth |
| CN109197388A (en) * | 2018-11-22 | 2019-01-15 | 山东友和菌业有限公司 | A kind of culture medium and preparation method of yellow needle mushroom |
| CN109906901A (en) * | 2019-04-10 | 2019-06-21 | 湖南素元生物科技有限公司 | Utilize the method for ultrasonic radiation planting rhizoma gastrodiae |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101434642A (en) * | 2007-11-15 | 2009-05-20 | 黑龙江大学 | Method for preparing non-denaturation hogskin insoluble collagen powder |
| CN103961749A (en) * | 2014-05-07 | 2014-08-06 | 无锡贝迪生物工程有限公司 | Method for preparing collagen protein/silica membrane double-layer stent |
| CN104447074A (en) * | 2014-12-30 | 2015-03-25 | 尤素梅 | Preparation method and application of green and environment-friendly and composite functional plant leaf fertilizer |
| CN104945118A (en) * | 2015-06-16 | 2015-09-30 | 王金生 | Method for preparing mushroom culture medium |
| CN105461395A (en) * | 2015-12-17 | 2016-04-06 | 凤台县千秋食用菌有限公司 | High-nitrogen mushroom culture medium and preparation method thereof |
-
2016
- 2016-08-09 CN CN201610645896.8A patent/CN106316578A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101434642A (en) * | 2007-11-15 | 2009-05-20 | 黑龙江大学 | Method for preparing non-denaturation hogskin insoluble collagen powder |
| CN103961749A (en) * | 2014-05-07 | 2014-08-06 | 无锡贝迪生物工程有限公司 | Method for preparing collagen protein/silica membrane double-layer stent |
| CN104447074A (en) * | 2014-12-30 | 2015-03-25 | 尤素梅 | Preparation method and application of green and environment-friendly and composite functional plant leaf fertilizer |
| CN104945118A (en) * | 2015-06-16 | 2015-09-30 | 王金生 | Method for preparing mushroom culture medium |
| CN105461395A (en) * | 2015-12-17 | 2016-04-06 | 凤台县千秋食用菌有限公司 | High-nitrogen mushroom culture medium and preparation method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107903103A (en) * | 2017-10-30 | 2018-04-13 | 穆世鹏 | A kind of culture substrate for promoting edible fungi growth |
| CN109197388A (en) * | 2018-11-22 | 2019-01-15 | 山东友和菌业有限公司 | A kind of culture medium and preparation method of yellow needle mushroom |
| CN109906901A (en) * | 2019-04-10 | 2019-06-21 | 湖南素元生物科技有限公司 | Utilize the method for ultrasonic radiation planting rhizoma gastrodiae |
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Application publication date: 20170111 |