CN106242770A - A kind of Lentinus Edodes collagen cultivation matrix utilizing yeast extraction waste material to prepare - Google Patents
A kind of Lentinus Edodes collagen cultivation matrix utilizing yeast extraction waste material to prepare Download PDFInfo
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- CN106242770A CN106242770A CN201610645664.2A CN201610645664A CN106242770A CN 106242770 A CN106242770 A CN 106242770A CN 201610645664 A CN201610645664 A CN 201610645664A CN 106242770 A CN106242770 A CN 106242770A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D1/00—Fertilisers containing potassium
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Pest Control & Pesticides (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Inorganic Chemistry (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of Lentinus Edodes collagen cultivation matrix utilizing yeast extraction waste material to prepare, be prepared by the raw materials in: beer yeast residue 49 51, yeast extract waste liquid 46, biogas residue 18 22, Folium Camelliae sinensis powder 8 10, sodium iron ethylenediaminetetraacetate 34, ferrous sulfate 12, potassium chlorate 23, medical stone powder 68, Corii Sus domestica waste material 40 42, pH2.5 acetum is appropriate, sodium hydroxide solution is appropriate, water is appropriate；Beer yeast residue, yeast are extracted the waste liquid raw material as mushroom cultivation substrate by the mushroom cultivation substrate of the present invention, the industrial garbage of beer yeast is made to have obtained the recycling treatment of system, decrease slag and effluent discharge simultaneously, it is beer yeast residue and a kind of new way of yeast extraction waste liquid secondary utilization, needed for the nutrient substance such as a large amount of nitrogen sources the most contained therein are also extremely suitable for the growth of Lentinus Edodes, preferable economic benefit can be brought.
Description
Technical field
The present invention relates to fungus growing technique field, particularly relate to a kind of Lentinus Edodes glue utilizing yeast extraction waste material to prepare
Former cultivation matrix.
Background technology
Lentinus Edodes is commonly called as China mushroom, is that mycophagy medical material is cultivated in a kind of famous using, has long cultivation in China
Historical tradition, the nutritive value abundant because of it and medical value and deeply liked by consumer, become the second adult in the world
The edible fungi of work cultivation, due to the fast development of industry, the raw material of Lentinus Edodes substituting stuff cultivation traditional matrices is the most exhausted, needs actively
Explore the approach substituting substrate.On the other hand, along with the raising of people's living standard, it is desirable to Lentinus Edodes nutritive value and medicinal
It is worth and also can be improved, so needing to provide the mushroom cultivation substrate of a kind of high-quality.
Collagen protein as a kind of biomacromolecule, have natural hypotoxicity, hypoimmunity, bootable mycelial growth with
And the preferable advantage such as biocompatibility, its extracting method is simple simultaneously, and raw material sources extensively and cheap, are discarded by slaughterhouse
Corii Sus domestica can carry out scale extraction, it has a very high potential as the New-type matrix material of cultivating champignon, but Non-crosslinked
The collagen protein of moditied processing often has that degradation rate is too fast, deformation temperature is low, easily shrink deformation and mechanical performance
The shortcomings such as deficiency, need by the cross-linking modified mechanical performance improving collagen protein and anti-degradation capability.
At present, the method that collagen-stabilizedization processes mainly is cross-linked by method physically or chemically.Wherein,
Physical method has vacuum dehydrothermal, ultraviolet and C ray crosslinking etc., and the advantage of these methods is not introduce toxic chemical substance,
Can keep the biocompatibility that collagen is good, but individually use physical crosslinking tend not to obtain homogeneous, preferably crosslinking strong
Degree, therefore Chemical Crosslinking Methods is more widely applied.
Common chemical cross-linking agent has aldehydes, Carbodiimides, bis-epoxy class material and the different hydrochlorate of cyanogen etc..Wherein,
Glutaraldehyde is the cross-linking agent that a class is widely used in collagen base biological material, can reduce the antigenicity of collagen, but glutaraldehyde is handed over
There is the reduction collagenous biological compatibility and be easily caused the potential danger such as collagen calcification in connection agent.Research shows, even if glutaraldehyde is residual
When staying mass concentration as little as 310mg/L the most toxic, its functional group not reacted or collagen drop under the effect of enzyme
These functional groups discharged during solution all may cause cell-cytotoxic reaction, works the mischief to cultivating bacterium.And 1-(3-dimethylamino
Propyl group)-3-ethyl-carbodiimide hydrochloride/N-hydroxy-succinamide cross-linking agent has spy nontoxic, that biocompatibility is good
Point, applies and can show the most excellent performance in the modification of collagen protein.
The application for a patent for invention of Publication No. 102617213A discloses a kind of utilization compression straw and prepares cultivating champignon base
The method of matter, this culture matrix uses weight ratio to be the thick wood flour of 25-35%, the thin wood flour of 15-25%, the pressure being impregnated with of 25-35%
Contracting stalk particle, the wheat bran of 10-15%, the Gypsum Fibrosum of 1.0-1.5% are mixed with and form, and this invention culture matrix content of lignin is relatively
The utilization of height, beneficially mushroom mycelium, uses agricultural production waste material as cultivation matrix raw material simultaneously, improves environment and saving
Cost, but the cultivation matrix of this disclosure of the invention uses raw material the most single, easily causes nutrient unbalanced, needs extra nutritional thing
The interpolation of matter, simultaneously the highest with the biocompatibility of Lentinus Edodes, the extension with mycelia is improved without promoting for mushroom growth microenvironment
Enter effect, without the raising of notable surcharge, limit the scale application of invention substrate.
Summary of the invention
The object of the invention is contemplated to make up the defect of prior art, it is provided that a kind of perfume (or spice) utilizing yeast extraction waste material to prepare
Mushroom collagen cultivation matrix.
The present invention is achieved by the following technical solutions:
A kind of Lentinus Edodes collagen cultivation matrix utilizing yeast extraction waste material to prepare, is prepared by the raw materials in: medicated beer ferment
Female residue 49-51, yeast extract waste liquid 4-6, biogas residue 18-22, Folium Camelliae sinensis powder 8-10, sodium iron ethylenediaminetetraacetate 3-4, ferrous sulfate
1-2, potassium chlorate 2-3, medical stone powder 6-8, Corii Sus domestica waste material 40-42, sodium chloride powder 1-3, pepsin preparation 1-2,1-(3-bis-
Methylaminopropyl)-3-ethyl-carbodiimide hydrochloride 1.6-2.0, N-hydroxy-succinamide 0.4-0.5,1% sodium chloride solution
In right amount, pH2.5 acetum is appropriate, sodium hydroxide solution is appropriate, water is appropriate.
Specifically comprising the following steps that of the described Lentinus Edodes collagen cultivation matrix preparation method utilizing yeast extraction waste material to prepare
(1) water is added after beer yeast residue, biogas residue, Folium Camelliae sinensis powder being sufficiently mixed loose to material humidity, retting fermentation 20-24
My god, fermentation material squeezed and filters, obtaining fermentation liquid and fermentation siccative, then fermentation liquid is individually taken out, collect bacterium the most afterwards
Mud precipitation and high fermentation clear liquid, make lyophilized powder by bacterium mud lyophilization, i.e. obtains ferment siccative, fermentation clear liquid and lyophilized powder
Standby；
(2) in step 1 gained fermentation clear liquid, add sodium iron ethylenediaminetetraacetate, ferrous sulfate, yeast extraction waste liquid, be heated to
75-77 DEG C, being stirred continuously chelating 23-27 minute, be cooled to room temperature afterwards, add potassium chlorate, stirring mixing, to uniformly, obtains battalion
Nutrient solution is standby；
(3) Corii Sus domestica waste material unhairing afterwash is shredded, join in 1% sodium chloride solution by the solid-to-liquid ratio of 1:5-6g/mL, soak
Clean with clear water after 5-6 hour, then it is molten that by the solid-to-liquid ratio of 1:15-18g/mL, gained skin bit is joined the acetic acid that pH value is 2.5
In liquid, and soak 8-10 hour under condition of ice bath, after completing, skin bit is smashed, now add the above-mentioned acetic acid of same volume
Solution, and add pepsin preparation, enzymolysis 12-15 hour, the most per hour stirring 5-7 minute, enzymolysis uses bilayer after terminating
Filtered through gauze, collects filtrate, is added thereto to sodium hydroxide solution regulation PH to 7-8, adds sodium chloride powder, stand 10-
12 hours, afterwards that solution is centrifugal 15-18 minute with 5000-5500 rev/min, collect precipitation, after drying, obtain collagen protein powder
Standby；
(4) by step 1 gained fermentation siccative, lyophilized powder, step 2 gained nutritional solution, step 3 gained collagen protein powder and wheat
After meal stone powder all mixes and is sufficiently stirred for, it is placed under 2-4 DEG C of environment, now adds 1-(3-dimethylamino-propyl)-3-ethyl
Carbodiimide hydrochloride and N-hydroxy-succinamide, cross-link 10-12 hour, be warmed to room temperature after completing, obtain Lentinus Edodes of the present invention
Cultivation matrix.
The invention have the advantage that
Substrate of the present invention by extracting the glue by having reduced immunogenicity, biodegradability to collagen protein in Corii Sus domestica waste material
Former albumen is applied in the preparation of cultivation matrix, use simultaneously 1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride/
N-hydroxy-succinamide carries out crosslinking Treatment as cross-linking agent to collagen, makes denaturation temperature and the resistance to enzymolysis ability of collagen protein
Improving, water absorption rate and swelling ratio reduce, and improve its heat stability and structural stability, can not only provide certain propping up for Lentinus Edodes
Support and growth interface, the porous network structure that the interlaced arrangement of collagenous fiber bundle within collagen protein is formed simultaneously, also have
It is beneficial to beneficial microorganism carry and nutritional labeling fixing, the most beneficially the adhesion of mushroom mycelium and moving into, for Lentinus Edodes
Growth provide environment suitable, that nutrient is sufficient.
Beer yeast residue, yeast are extracted former as mushroom cultivation substrate of waste liquid by the mushroom cultivation substrate of the present invention
Material, makes the industrial garbage of beer yeast obtain the recycling treatment of system, decreases slag and effluent discharge simultaneously, is
The nutrition such as beer yeast residue and yeast extract a kind of new way that waste liquid secondary utilizes, a large amount of nitrogen sources the most contained therein
Needed for material is also extremely suitable for the growth of Lentinus Edodes, preferable economic benefit can be brought.
Detailed description of the invention
A kind of Lentinus Edodes collagen cultivation matrix utilizing yeast extraction waste material to prepare, by the raw material system of following weight portion (kg)
Become: beer yeast residue 49, yeast extract waste liquid 4, biogas residue 18, Folium Camelliae sinensis powder 8, sodium iron ethylenediaminetetraacetate 3, ferrous sulfate 1, chlorine
Acid potassium 2, medical stone powder 6, Corii Sus domestica waste material 40, sodium chloride powder 1, pepsin preparation 1,1-(3-dimethylamino-propyl)-3-second
Base carbodiimide hydrochloride 1.6, N-hydroxy-succinamide 0.4,1% sodium chloride solution are appropriate, pH2.5 acetum is appropriate, hydrogen
Sodium hydroxide solution is appropriate, water is appropriate.
Specifically comprising the following steps that of the described Lentinus Edodes collagen cultivation matrix preparation method utilizing yeast extraction waste material to prepare
(1) adding water after beer yeast residue, biogas residue, Folium Camelliae sinensis powder being sufficiently mixed loose to material humidity, retting is fermented 20 days, will
Fermentation material squeezes and filters, and obtains fermentation liquid and fermentation siccative, is more individually taken out by fermentation liquid, collects bacterium mud precipitation the most afterwards
With high fermentation clear liquid, lyophilized powder is made in bacterium mud lyophilization, i.e. obtain fermentation siccative, fermentation clear liquid and lyophilized powder standby；
(2) in step 1 gained fermentation clear liquid, add sodium iron ethylenediaminetetraacetate, ferrous sulfate, yeast extraction waste liquid, be heated to
75 DEG C, being stirred continuously chelating 23 minutes, be cooled to room temperature afterwards, add potassium chlorate, stirring mixing, to uniformly, obtains nutritional solution standby
With；
(3) Corii Sus domestica waste material unhairing afterwash is shredded, join in 1% sodium chloride solution by the solid-to-liquid ratio of 1:5g/mL, soak 5 little
Shi Houyong clear water is cleaned, then is joined in the acetum that pH value is 2.5 by the solid-to-liquid ratio of 1:15g/mL by gained skin bit, and
Soak 8 hours under condition of ice bath, after completing, skin bit is smashed, now add the above-mentioned acetum of same volume, and add
Pepsin preparation, enzymolysis 12 hours, the most per hour stirring 5 minutes, enzymolysis filters with double gauze after terminating, collects filter
Liquid, be added thereto to sodium hydroxide solution regulation PH to 7, add sodium chloride powder, stand 10 hours, afterwards by solution with
5000 revs/min are centrifuged 15 minutes, collect precipitation, obtain collagen protein powder standby after drying；
(4) by step 1 gained fermentation siccative, lyophilized powder, step 2 gained nutritional solution, step 3 gained collagen protein powder and wheat
After meal stone powder all mixes and is sufficiently stirred for, it is placed under 2 DEG C of environment, now adds 1-(3-dimethylamino-propyl)-3-ethyl carbon
Diimmonium salt hydrochlorate and N-hydroxy-succinamide, cross-link 10 hours, be warmed to room temperature after completing, obtain cultivating champignon base of the present invention
Matter.
In order to further illustrate the using value of the present invention, inventor uses substrate of the present invention and commercially available generic media respectively
Carrying out the cultivating and growing of Lentinus Edodes, in addition to using substrate difference, other management methods are the most identical, and data measured is substrate of the present invention institute
The more conventional substrate of biological transformation ratio of Lentinus Edodes improves 5.0%, and the output increased of finished product mushroom 17.5%, massee fruiting bodies simultaneously
Stem is pure white, does not has misshapen mushroom, illustrates that Lentinus Edodes substrate of the present invention has preferable using effect.
Claims (2)
1. one kind utilizes yeast to extract the Lentinus Edodes collagen cultivation matrix prepared of waste material, it is characterised in that former by following weight portion
Material is made: beer yeast residue 49-51, yeast extract waste liquid 4-6, biogas residue 18-22, Folium Camelliae sinensis powder 8-10, EDTA-Fe
Sodium 3-4, ferrous sulfate 1-2, potassium chlorate 2-3, medical stone powder 6-8, Corii Sus domestica waste material 40-42, sodium chloride powder 1-3, pepsin
Preparation 1-2,1-(3-dimethylamino-propyl)-3-ethyl-carbodiimide hydrochloride 1.6-2.0, N-hydroxy-succinamide 0.4-
0.5,1% sodium chloride solution is appropriate, pH2.5 acetum is appropriate, sodium hydroxide solution is appropriate, water is appropriate.
2. extract Lentinus Edodes collagen cultivation matrix prepared by waste material according to the yeast that utilizes described in claims 1, it is characterised in that
Specifically comprising the following steps that of preparation method
(1) water is added after beer yeast residue, biogas residue, Folium Camelliae sinensis powder being sufficiently mixed loose to material humidity, retting fermentation 20-24
My god, fermentation material squeezed and filters, obtaining fermentation liquid and fermentation siccative, then fermentation liquid is individually taken out, collect bacterium the most afterwards
Mud precipitation and high fermentation clear liquid, make lyophilized powder by bacterium mud lyophilization, i.e. obtains ferment siccative, fermentation clear liquid and lyophilized powder
Standby；
(2) in step 1 gained fermentation clear liquid, add sodium iron ethylenediaminetetraacetate, ferrous sulfate, yeast extraction waste liquid, heating
To 75-77 DEG C, being stirred continuously chelating 23-27 minute, be cooled to room temperature afterwards, add potassium chlorate, stirring mixing, to uniformly, obtains
Nutritional solution is standby；
(3) Corii Sus domestica waste material unhairing afterwash is shredded, join in 1% sodium chloride solution by the solid-to-liquid ratio of 1:5-6g/mL, soak
Clean with clear water after 5-6 hour, then it is molten that by the solid-to-liquid ratio of 1:15-18g/mL, gained skin bit is joined the acetic acid that pH value is 2.5
In liquid, and soak 8-10 hour under condition of ice bath, after completing, skin bit is smashed, now add the above-mentioned acetic acid of same volume
Solution, and add pepsin preparation, enzymolysis 12-15 hour, the most per hour stirring 5-7 minute, enzymolysis uses bilayer after terminating
Filtered through gauze, collects filtrate, is added thereto to sodium hydroxide solution regulation PH to 7-8, adds sodium chloride powder, stand 10-
12 hours, afterwards that solution is centrifugal 15-18 minute with 5000-5500 rev/min, collect precipitation, after drying, obtain collagen protein powder
Standby；
(4) by step 1 gained fermentation siccative, lyophilized powder, step 2 gained nutritional solution, step 3 gained collagen protein powder and
After medical stone powder all mixes and is sufficiently stirred for, it is placed under 2-4 DEG C of environment, now adds 1-(3-dimethylamino-propyl)-3-second
Base carbodiimide hydrochloride and N-hydroxy-succinamide, cross-link 10-12 hour, be warmed to room temperature after completing, and obtains the present invention fragrant
Mushroom cultivation matrix.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610645664.2A CN106242770A (en) | 2016-08-09 | 2016-08-09 | A kind of Lentinus Edodes collagen cultivation matrix utilizing yeast extraction waste material to prepare |
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| CN201610645664.2A CN106242770A (en) | 2016-08-09 | 2016-08-09 | A kind of Lentinus Edodes collagen cultivation matrix utilizing yeast extraction waste material to prepare |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110128213A (en) * | 2019-06-19 | 2019-08-16 | 乐斯福(明光)有限公司 | A method of liquid fertilizer is produced using beer waste yeast |
| CN114032623A (en) * | 2022-01-10 | 2022-02-11 | 天新福（北京）医疗器材股份有限公司 | Preparation process of high-yield collagen fibers |
| CN114847077A (en) * | 2022-04-18 | 2022-08-05 | 百色学院 | Method for treating yeast industrial wastewater by using edible fungus production technology |
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| CN102716516A (en) * | 2012-05-11 | 2012-10-10 | 天津大学 | Polydatin modified collagen scaffold, and preparation method and application thereof |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110128213A (en) * | 2019-06-19 | 2019-08-16 | 乐斯福(明光)有限公司 | A method of liquid fertilizer is produced using beer waste yeast |
| CN114032623A (en) * | 2022-01-10 | 2022-02-11 | 天新福（北京）医疗器材股份有限公司 | Preparation process of high-yield collagen fibers |
| CN114032623B (en) * | 2022-01-10 | 2022-03-29 | 天新福（北京）医疗器材股份有限公司 | Preparation process of high-yield collagen sponge |
| CN114847077A (en) * | 2022-04-18 | 2022-08-05 | 百色学院 | Method for treating yeast industrial wastewater by using edible fungus production technology |
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Application publication date: 20161221 |