CN103808710A - Chemiluminescent detection equipment and method - Google Patents
Chemiluminescent detection equipment and method Download PDFInfo
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- CN103808710A CN103808710A CN201410028514.8A CN201410028514A CN103808710A CN 103808710 A CN103808710 A CN 103808710A CN 201410028514 A CN201410028514 A CN 201410028514A CN 103808710 A CN103808710 A CN 103808710A
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Abstract
The invention discloses chemiluminescent detection equipment and method. The chemiluminescent detection equipment comprises a receiving unit, an acquiring unit, a photon counting unit and a display unit, wherein the receiving unit is used for receiving a to-be-detected object selected by a user; the acquiring unit is used for acquiring the to-be-detected object; the photon counting unit is used for shaping photon generated by the to-be-detected object into a pulse signal and performing pulse counting treatment on the pulse signal; the display unit is used for displaying a numerical value of the pulse counting to the user. Through the chemiluminescent detection equipment and method disclosed by the invention, the manufacturing cost and the detection cost can be lowered; besides, the chemiluminescent detection equipment and method are simple to operate.
Description
Technical field
The invention belongs to chemical detection devices technical field, specifically, relate to a kind of chemiluminescence detection equipment and method.
Background technology
Chemiluminescence immune assay is worldwide to develop nearly ten years very fast on-radiation immunoassay, is continue enzyme immune technology, puts the supersensitive trace measurement technology of the one growing up after immune technology, immunofluorence technic.It is a kind of immune analysis method that utilizes the direct labelled antigen of chemiluminescence agent or antibody.Generally comprise two parts, i.e. immune response system and chemiluminescence analysis system.And chemiluminescence analysis system is to utilize chemiluminescent substance through the catalysis of catalyzer and the oxidation of oxygenant, form the intermediate of an excited state, in the time that this excited state intermediate is got back to stable ground state, launch photon (hM) simultaneously, utilize luminous signal surveying instrument to measure quantum yield of luminscence.
At present, in the prior art, equipment is mainly produced chemiluminescence immune assay by companies such as Baeyer, Abbott Laboratories, Beckman, Depews.Although these instrumentation sets the ultrasound wave antipollution technology of advanced computer-controlled full-automatic analytical technology, stable enhanced chemiluminescence technique particulate immunity solid phase assays technology and unique efficient, have random, continuously, the advantage such as urgent sampling.But, the cost of these instruments is higher, testing cost is higher, make to be subject to larger restriction in application, for example, the price of novel VIDAS instrument is entered 600,000 yuan, and the detection kit that this instrument must coordinate French import Mei Liai company to produce detects, as calculated, the expense of a bacterium of every detection is greatly about 30～50 yuan.Therefore, need to provide a kind of cost lower, the chemiluminescence detection equipment that testing cost is lower.
Summary of the invention
The chemiluminescence detection equipment and the method that provide a kind of cost lower are provided one object of the present invention.
The chemiluminescence detection equipment and the method that provide a kind of testing cost lower are provided.
According to an aspect of the present invention, provide a kind of chemiluminescence detection equipment, described chemiluminescence detection equipment comprises: receiving element, receives the target to be detected that user selects; Acquiring unit, obtains target to be detected; Photon counting unit, the photon that target to be detected is produced is shaped to pulse signal, and pulse signals is carried out step-by-step counting; Display unit, shows the numerical value of described step-by-step counting to user.
According to an aspect of the present invention, described photon counting unit can comprise: photomultiplier, converts electric signal to for the photon that target to be detected is produced; Shaping unit, for being shaped to pulse signal by electric signal; Impulse meter, pulse signals is carried out step-by-step counting.
According to an aspect of the present invention, described shaping unit comprises: operational amplifier and the comparer being electrically connected with operational amplifier, and so that electric signal is shaped to pulse signal.
According to an aspect of the present invention, utilize flyback transformer to power to photomultiplier.
According to an aspect of the present invention, described acquiring unit comprises two sensors, and by controlling two sensors to obtain target to be detected.
According to an aspect of the present invention, drive two sensors by L297 stepper motor driver and L298 stepper motor driver, to obtain target to be detected.
According to an aspect of the present invention, described target to be detected is positioned at 96 orifice plates.
According to an aspect of the present invention, the wavelength of described photomultiplier is 200～1200 nanometers.
According to a further aspect in the invention, provide a kind of chemical luminescence detection method, described chemical luminescence detection method comprises: receive the target to be detected that user selects; Obtain target to be detected; The photon that target to be detected is produced is shaped to pulse signal, and pulse signals is carried out step-by-step counting; Show the numerical value of described step-by-step counting to user, to measure the quantity of photon.
According to a further aspect in the invention, described chemical luminescence detection method comprises: receive the target to be detected that user selects; Obtain target to be detected; The photon that photomultiplier produces the target to be detected receiving converts electric signal to; By operational amplifier and comparer, electric signal is shaped to pulse signal; Carry out step-by-step counting by impulse meter pulse signals; And show the numerical value of described step-by-step counting to user, to measure the quantity of photon.
According to chemiluminescence detection equipment of the present invention and method, can reduce cost and testing cost.In addition, this chemiluminescence detection equipment and method, simple to operate.
Accompanying drawing explanation
By the description to embodiment of carrying out below in conjunction with accompanying drawing, above-mentioned and/or other object of the present invention and advantage will become apparent, wherein:
Fig. 1 illustrates the block diagram of chemiluminescence detection equipment according to an exemplary embodiment of the present invention;
Fig. 2 illustrates the block diagram of the photon counting unit of chemiluminescence detection equipment according to an exemplary embodiment of the present invention;
Fig. 3 illustrates the process flow diagram of chemical luminescence detection method according to an exemplary embodiment of the present invention;
Fig. 4 illustrates the process flow diagram of the chemical luminescence detection method of another exemplary embodiment according to the present invention;
Fig. 5 illustrates the acquiring unit circuit diagram of chemiluminescence detection equipment according to an exemplary embodiment of the present invention;
Fig. 5 A illustrates the A place partial enlarged drawing shown in Fig. 5;
Fig. 5 B illustrates the B place partial enlarged drawing shown in Fig. 5;
Fig. 5 C illustrates the C place partial enlarged drawing shown in Fig. 5;
Fig. 5 D illustrates the D place partial enlarged drawing shown in Fig. 5;
Fig. 6 illustrates the photon counting element circuit figure of chemiluminescence detection equipment according to an exemplary embodiment of the present invention;
Fig. 7 illustrates the photomultiplier power supply circuit of chemiluminescence detection equipment according to an exemplary embodiment of the present invention;
Fig. 8 illustrates the signal graph of the pulse signal after electric signal and the shaping that the photomultiplier of chemiluminescence detection equipment is exported according to an exemplary embodiment of the present invention, and wherein, a is pulse signal, and b is electric signal;
Fig. 9 illustrates the figure of the pulse rise time of the pulse signal of chemiluminescence detection equipment according to an exemplary embodiment of the present invention;
Figure 10 illustrates the width of the electric signal of the photomultiplier output of chemiluminescence detection equipment according to an exemplary embodiment of the present invention;
Figure 11 illustrates the width of the pulse signal after the shaping of chemiluminescence detection equipment according to an exemplary embodiment of the present invention.
Embodiment
Now will be in detail with reference to embodiments of the invention, the example of described embodiment is shown in the drawings, and wherein, identical label refers to identical parts all the time.Below will be by described embodiment is described with reference to accompanying drawing, to explain the present invention.
Fig. 1 illustrates the block diagram of chemiluminescence detection equipment according to an exemplary embodiment of the present invention.Fig. 2 illustrates the block diagram of the photon counting unit of chemiluminescence detection equipment according to an exemplary embodiment of the present invention.Fig. 3 illustrates the process flow diagram of chemical luminescence detection method according to an exemplary embodiment of the present invention.Fig. 4 illustrates the process flow diagram of the chemical luminescence detection method of another exemplary embodiment according to the present invention.Fig. 5 illustrates the acquiring unit circuit diagram of chemiluminescence detection equipment according to an exemplary embodiment of the present invention.Fig. 5 A illustrates the A place partial enlarged drawing shown in Fig. 5.Fig. 5 B illustrates the B place partial enlarged drawing shown in Fig. 5.Fig. 5 C illustrates the C place partial enlarged drawing shown in Fig. 5.Fig. 5 D illustrates the D place partial enlarged drawing shown in Fig. 5.Fig. 6 illustrates the photon counting element circuit figure of chemiluminescence detection equipment according to an exemplary embodiment of the present invention.Fig. 7 illustrates the photomultiplier power supply circuit of chemiluminescence detection equipment according to an exemplary embodiment of the present invention.Fig. 8 illustrates the signal graph of the pulse signal after electric signal and the shaping that the photomultiplier of chemiluminescence detection equipment is exported according to an exemplary embodiment of the present invention.Fig. 9 illustrates the figure of the pulse rise time of the pulse signal of chemiluminescence detection equipment according to an exemplary embodiment of the present invention.Figure 10 illustrates the width of the electric signal of the photomultiplier output of chemiluminescence detection equipment according to an exemplary embodiment of the present invention.Figure 11 illustrates the width of the pulse signal after the shaping of chemiluminescence detection equipment according to an exemplary embodiment of the present invention.
Fig. 1 illustrates the block diagram of chemiluminescence detection equipment according to an exemplary embodiment of the present invention.As shown in Figure 1, chemiluminescence detecting comprises according to an exemplary embodiment of the present invention: receiving element 10, acquiring unit 20, photon counting unit 30, display unit 40.
Specifically, the target to be detected that receiving element 10 is selected for receiving user.As example, target to be detected described here can be the one in Bacterium enteritidis, Salmonella choleraesuls and salmonella typhimurium.In addition preferably, target to be detected can be positioned in 96 orifice plates (i.e. 96 porocyte culture plates or 96 porocyte double dish).In addition, user can select to need in 96 orifice plates the target location to be detected detected (for example, 96 orifice plates can be divided into X coordinate and Y coordinate, target location to be detected is determined in position by input X coordinate and Y coordinate), by the various operations indication that makes one's options, for example, key-press input, keyboard input, touch input etc.
Photomultiplier 31 converts electric signal to for the photon that target to be detected is produced.As example, in the present invention, the wavelength that the model that adopts Bin Song company to produce is CR110 is at the photomultiplier of 200～1200 nanometers, and photomultiplier can comprise photocathode, electron optics input system, electron multiplication system, anode.As preferred mode, utilize flyback transformer to power to photomultiplier, the high pressure that is 1000V by the voltage transitions of 18V is powered to photomultiplier, as shown in Figure 7.In addition,, in order to prevent that high pressure from puncturing resistance, preferably, 10 resistance (R2～R11 as shown in Figure 7) series connection is carried out to dividing potential drop to it.
Referring to Fig. 3, chemical luminescence detection method is according to an exemplary embodiment of the present invention described.
With reference to Fig. 3, at step S100, utilize receiving element 10 to receive the target to be detected that user selects.Target to be detected can be positioned in 96 orifice plates (i.e. 96 porocyte culture plates or 96 porocyte double dish).User can select to need in 96 orifice plates the target location to be detected of detection, for example, 96 orifice plates can be divided into X coordinate and Y coordinate, determines target location to be detected by the position of input X coordinate and Y coordinate.In addition, by the various operations indication that makes one's options, for example, key-press input, keyboard input, touch input etc.
Next,, at step S200, obtain target to be detected by acquiring unit 20.Preferred mode, can drive two sensors to obtain the coordinate position of the target to be detected that receiving element 10 receives by L297 stepper motor driver and L298 stepper motor driver, to obtain target to be detected.
Then, at step S300, photon target to be detected being produced by photon counting unit 30 is shaped to pulse signal, and pulse signals is carried out step-by-step counting.
Then, at step S400, shown the numerical value of described step-by-step counting to user by display unit 40, to measure the quantity of photon.
Fig. 4 illustrates the process flow diagram of the chemical luminescence detection method of another exemplary embodiment according to the present invention.
With reference to Fig. 4, at step S100, utilize receiving element 10 to receive the target to be detected that user selects.Then,, at step S200, obtain target to be detected by acquiring unit 20.
Next,, at step S310, the photon that photomultiplier produces the target to be detected receiving converts electric signal to.At step S320, by operational amplifier and comparer, electric signal is shaped to pulse signal.At step S330, carry out step-by-step counting by impulse meter pulse signals.
Here, photoelectron arrives anode and has a time delay from the photocathode transmitting of photomultiplier, and this time delay is called the transit time.Due to each photoelectronic transit time difference, therefore also can there is transit time spread (TTS).The time response of photomultiplier represents by pulse rise time and response pulse duration conventionally.It is pointed out that and refer to that pulse height rose to for 90% required time from 10% pulse rise time.Response pulse duration refers to the time interval of pulse height between being 50% 2.Affect the amplitude of photomultiplier output electrical signals pulse rise time; Response pulse duration determines whether to produce pile-up effect.Pulse signal after electric signal and the shaping of photomultiplier output as shown in Figure 8.
Experiment measuring the pulse rise time of 3016 pulse signals.As shown in Figure 9, the mean value of the pulse rise time of pulse signal is 30ns, and be 2.2ns the pulse rise time of the electric signal (, the pulse signal before shaping) of photomultiplier output.Experiment shows, photomultiplier meets photon counting unit and carries out the requirement that pulse signal is counted.
Experiment measuring the width of pulse signal after width and the shaping of electric signal of photomultiplier output, as shown in Figure 10 and Figure 11.As can be seen from Figure 10, the statistical property of the electric signal of electric multiplier tube output is Gaussian distribution, and average is 53.6ns, σ=6.3ns.As can be seen from Figure 11, the average of the pulse signal after shaping is 89.3ns, σ=26.5ns.Through contrast, the width of the pulse signal after shaping is obviously large than the width of electric signal, and this width that shows the pulse signal that electric signal exports after shaping unit carries out shaping spreads to some extent, meets photon counting unit and carries out the requirement that pulse signal is counted
Then, at step S400, show the numerical value of described step-by-step counting to user, to measure the quantity of photon.
According to chemiluminescence detection equipment of the present invention and method, can reduce cost and testing cost.In addition, this chemiluminescence detection equipment and method, simple to operate.
Above each embodiment of the present invention is only exemplary, and the present invention is not limited to this.Those skilled in the art should understand that: without departing from the principles and spirit of the present invention, can change these embodiments, that is to say, variation in any form or details still falls among scope of the present invention, wherein, scope of the present invention limits in claim and equivalent thereof.
Claims (10)
1. a chemiluminescence detection equipment, described chemiluminescence detection equipment comprises:
Receiving element, receives the target to be detected that user selects;
Acquiring unit, obtains target to be detected;
Photon counting unit, the photon that target to be detected is produced is shaped to pulse signal, and pulse signals is carried out step-by-step counting;
Display unit, shows the numerical value of described step-by-step counting to user.
2. chemiluminescence detection equipment according to claim 1, is characterized in that, described photon counting unit comprises:
Photomultiplier, converts electric signal to for the photon that target to be detected is produced;
Shaping unit, for being shaped to pulse signal by electric signal;
Impulse meter, pulse signals is carried out step-by-step counting.
3. chemiluminescence detection equipment according to claim 1, is characterized in that, described shaping unit comprises: operational amplifier and the comparer being electrically connected with operational amplifier, and so that electric signal is shaped to pulse signal.
4. chemiluminescence detection equipment according to claim 2, is characterized in that, utilizes flyback transformer to power to photomultiplier.
5. chemiluminescence detection equipment according to claim 1, is characterized in that, described acquiring unit comprises two sensors, and by controlling two sensors to obtain target to be detected.
6. chemiluminescence detection equipment according to claim 5, is characterized in that, drives two sensors by L297 stepper motor driver and L298 stepper motor driver, to obtain target to be detected.
7. according to the chemiluminescence detection equipment described in any one in claim 1～6, it is characterized in that, described target to be detected is positioned at 96 orifice plates.
8. according to the chemiluminescence detection equipment described in any one in claim 1～6, it is characterized in that, the wavelength of described photomultiplier is 200～1200 nanometers.
9. a chemical luminescence detection method, comprising:
Receive the target to be detected that user selects;
Obtain target to be detected;
The photon that target to be detected is produced is shaped to pulse signal, and pulse signals is carried out step-by-step counting;
Show the numerical value of described step-by-step counting to user, to measure the quantity of photon.
10. chemical luminescence detection method according to claim 9, is characterized in that, described chemical luminescence detection method comprises:
Receive the target to be detected that user selects;
Obtain target to be detected;
The photon that photomultiplier produces the target to be detected receiving converts electric signal to;
By operational amplifier and comparer, electric signal is shaped to pulse signal;
Carry out step-by-step counting by impulse meter pulse signals; And
Show the numerical value of described step-by-step counting to user, to measure the quantity of photon.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108362898A (en) * | 2018-03-02 | 2018-08-03 | 江西英大生物技术有限公司 | Full-automatic chemiluminescence immunoassay analysis meter |
| CN109983761A (en) * | 2016-11-25 | 2019-07-05 | 浜松光子学株式会社 | Photon detector |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109983761A (en) * | 2016-11-25 | 2019-07-05 | 浜松光子学株式会社 | Photon detector |
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