CN103805487A - Preparation method of seaweed wine - Google Patents

Preparation method of seaweed wine Download PDF

Info

Publication number
CN103805487A
CN103805487A CN201410075136.9A CN201410075136A CN103805487A CN 103805487 A CN103805487 A CN 103805487A CN 201410075136 A CN201410075136 A CN 201410075136A CN 103805487 A CN103805487 A CN 103805487A
Authority
CN
China
Prior art keywords
wine
supernatant liquor
additive
gets
aside
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201410075136.9A
Other languages
Chinese (zh)
Other versions
CN103805487B (en
Inventor
王培磊
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shenzhen space microalgae Biotechnology Co.,Ltd.
Original Assignee
Linyi University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Linyi University filed Critical Linyi University
Priority to CN201410075136.9A priority Critical patent/CN103805487B/en
Publication of CN103805487A publication Critical patent/CN103805487A/en
Application granted granted Critical
Publication of CN103805487B publication Critical patent/CN103805487B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Landscapes

  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Alcoholic Beverages (AREA)

Abstract

The invention provides a preparation method of seaweed wine. The preparation method of seaweed wine comprises the following steps of: step 1, dissolving 2.0g of chlorella dry algae powder in 500ml of distilled water, stirring completely or shaking up for 45min to extract water soluble vitamin, micro seaweed polysaccharide and CGF active factor; filtering with bolting silk, removing sediments and foam, then extracting the supernate in a centrifugal way, taking the supernate and adding the first group of additives; step 2, dissolving 3.0g of chlorella dry algae powder in 500ml of 95% ethyl alcohol, stirring completely or shaking up to be uniform for 60min to extract alcohol-soluble components; filtering with bolting silk, deslagging and keeping the spare liquor; centrifugal precipitation, taking the supernate and adding the second group of additives; and step 3, adding liquor A and liquor B to wine base (or raw wine), mixing, precipitating, and bottling. The method is simple in process and equipment, high in efficiency, easy to operate, mature in technology and huge in market potential and uses abundant raw materials. The seaweed wine can be prepared in wine plants in any scales or individual wine plants.

Description

A kind of making method of marine alga wine
Technical field
The invention belongs to health promoting wine field, relate in particular to a kind of making method of marine alga wine.
Background technology
Chlorella is the unicellular Pandorinopsis microorganism of the deep green of one way of life in ocean, and diameter 3-8 micron is similar to human erythrovirus volume, is therefore called again " green blood cell ".Strong to salinity adaptation, can orthobiosis in brackish water and fresh water through domestication.Protein content is 2 times of beef, 3 times of chicken; Irony content is 50 times of raisin, 16 times of pork liver; Calcareous is 2 times of milk; Vitamin A content is 4 times of raisin; Carotene is 100 times of general plant; Chlorophyll is 5 times of spirulina; Containing the vitamin B12 higher than foie gras.Therefore, chlorella is enjoyed the laudatory title of " nutrient for plants+meat quality protein ", and nutritive value is considerably beyond high protein foodstuffs such as egg, beef and soybean.The multiple amino acids that chlorella is contained and lipoprotein, can activating human body cells, repair the impaired gene of human body, promotes the growth of organ-tissue.Water-soluble and the alcohol soluble vitamin containing can be effectively anti-oxidant, remove interior free yl, improve the heart, pulmonary function, the effect of playing beauty treatment and delaying senility.Also contain polysaccharides and various trace elements the same as spirulina, can antifatigue, atherosclerosis.The exclusive CGF active factor of chlorella can improve immunizing power, effectively repair body injury, strengthens cytophagy ability.The linoleic acid plus linolenic acid of chlorella can regulate blood fat, blood viscosity, efficient balance nutrition and eliminating toxin, and prevent multiple chronic disease.Plant cellulose in chlorella can promote gastrointestinal peristalsis, accelerates food digestion, can also get rid of chemical toxicant, heavy metal etc. in body, makes human body more relaxed and comfortable.
Traditional marine alga wine process for making is first the kelp such as sea-tangle, wakame to be shredded, add respectively acid, alkali, extraction using alcohol, three kinds of extracts are mixed to get to seaweed extracted liquor, this extracting solution is added by a certain percentage in wine-making technology process, marine alga wine is made in fermentation.This wine is mainly to utilize sea-tangle, the kelps such as wakame are made raw material, technique is coarse, cost is higher, nutritive ingredient dullness, to the chlorophyll in marine alga, Sargassum polysaccharides, amino acid, VITAMIN, phycocolloid, sterol, macrolide, terpene, alkaloid, the extraction efficiencies such as unsaturated fatty acids are lower, and the present invention utilizes more marine microalgae one chlorella of horn of plenty of resource, by distilled water and the extraction of ethanol secondary, water-soluble and alcohol dissolubility organic composition in chlorella is fully extracted, to greatest extent the lipoprotein in chlorella, β-carotene, chlorophyll a, VITAMIN, gamma-linolenic acid, micro-polysaccharides and CGF active factor etc. extract.After measured, 85% lipoprotein in algae powder, 74% gamma-linolenic acid, 88% β-carotene can be extracted in wine.Compared with traditional direct immersion, the effective rate of utilization of lipoprotein brings up to 85% from 21%, the percentage extraction of gamma-linolenic acid brings up to 74% from traditional 29%, and in extraction process, do not use acid, the material that alkali etc. are harmful, only use distilled water, the ethanol that grain fermentation produces, more natural, environmental protection, efficiently, nutritive ingredient more comprehensively, mouthfeel is good, color and luster is limpid, lipoprotein content is high, technical process is simple, device simple, less investment, cost is low, instant effect, high efficiency, easy handling, with short production cycle, aboundresources, technology maturation, reliable product quality.Through market survey and inspection information, also do not find that there is the health promoting wine of making take chlorella as raw material now, in other words, it is blank that this produce market still belongs to, and market potential is huge, and large and small brewery or individual brewery all can produce, and traditional brewery transforms a little also and can brewage.
Summary of the invention
In order to overcome the deficiency of current technology, the invention provides a kind of marine alga wine production method, concrete technical scheme is as follows:
A kind of marine alga wine making method, this marine alga wine making step is as follows:
The first step, gets chlorella dry algae powder 2.0g and is dissolved in 500ml distilled water, fully stirs or shake up 45min, comes extraction water soluble vitamin, micro-polysaccharides and CGF active factor, and supernatant liquor is sky blue; With the silk cover filtering of mesh aperture 100 μ m, throw aside after sediment and foam, by the centrifugal 10min of whizzer for supernatant liquor, rotating speed 4500r/min, throws aside throw out, leaves supernatant liquor, leave standstill 48h, again throw aside sediment, get supernatant liquor and add first group of additive, wherein to add amount and the component of first group of additive as follows for every 1000ml supernatant liquor:
Then fully shake up, obtain A liquid, 4 ℃ of Refrigerator stores, for subsequent use;
Second step, gets chlorella dry algae powder 3.0g and is dissolved in the ethanol of 500m195%, fully stirs or shake up 60min, extracts lipoprotein, β-carotene, chlorophyll a, alcohol soluble vitamin, these Alcohol soluble compositions of gamma-linolenic acid, and supernatant liquor is grass green; With the silk cover filtering of mesh aperture 80-100 μ m, remove after sediment, leave clear liquid; By whizzer centrifugal 5min for clear liquid, rotating speed 5000r/min, throws aside throw out, gets supernatant liquor and leaves standstill 60h, again throws aside sediment, gets supernatant liquor, adds second group of additive, and wherein to add amount and the component of second group of additive as follows for every 1000ml supernatant liquor:
Figure BSA0000101570810000022
Figure BSA0000101570810000031
Then fully shake up, obtain B liquid, 4 ℃ of Refrigerator stores, for subsequent use;
The 3rd step, gets wine base (or former wine) 500ml, adds A liquid 30ml, fully stirs or shake up 25min; Add again B liquid 20ml, fully shake up or stir 45min, now have a small amount of white flocks; With the silk cover filtering of mesh aperture 60-100 μ m, throw aside cotton-shaped dregs and foam, get the centrifugal 10min of clear liquid, rotating speed 5000r/min, throws aside throw out, gets supernatant liquor, leaves standstill 72h, again throws aside throw out, gets supernatant liquor, and bottling completes.
The additive using in described a kind of marine alga wine making method, this binder component and proportioning are as follows:
Figure BSA0000101570810000032
The additive using in described a kind of marine alga wine making method, this binder component and proportioning are as follows:
Figure BSA0000101570810000033
Beneficial effect: the trace element that marine alga wine contains the abundant needed by human such as iodine, selenium, vinosity is mellow, transparent limpid.Because the vinosity that it is unique and nutritive value are harmless to the person of being addicted to drink, therefore there is good market popularization value.Chlorella can be removed interior free yl, improved the heart, pulmonary function, improve looks, delay senility, antifatigue, atherosclerosis, exclusive CGF active factor can improve immunizing power, repairs body injury, strengthen cytophagy ability.Traditional marine alga wine making method is by sea-tangle, the kelp choppings such as wakame, acid adding, alkali, extraction using alcohol, technique is coarse, cost is higher, nutrition is single, to Sargassum polysaccharides, phycocolloid, sterol, macrolide, terpene, the extraction efficiencies such as alkaloid are lower, and the present invention is take chlorella as main raw material, by distilled water and the extraction of ethanol secondary, chlorella lipoprotein utilization ratio brings up to 85% from 21%, gamma-linolenic acid percentage extraction brings up to 74% from 29%, nutrition more comprehensively, mouthfeel is good, color and luster is limpid, lipoprotein content is high, technical process is simple, device simple, high efficiency, easy handling, abundant raw material, technology maturation, market potential is huge, size brewery or individual brewery all can produce.
Accompanying drawing explanation
Fig. 1 is the preparation flow figure of A liquid
Fig. 2 is the preparation flow figure of B liquid
Fig. 3 is marine alga algae wine fabrication processing figure
Embodiment
A kind of marine alga wine making method, is characterized in that this marine alga wine making step is as follows:
The first step
Get chlorella dry algae powder 2.0g and be dissolved in 500ml distilled water, fully stir or shake up 45min.Be mainly extraction water soluble vitamin, micro-polysaccharides and CGF active factor, supernatant liquor is sky blue.With the silk cover filtering of mesh aperture 100 μ m, throw aside after sediment and foam, by the centrifugal 10min of whizzer for clear liquid, rotating speed 4500r/min, get supernatant liquor and leave standstill 48h, throw aside throw out, leave supernatant liquor, add first group of additive (every 1000ml supernatant liquor addition is as follows), fully shake up, obtain A liquid, 4 ℃ of Refrigerator stores, for subsequent use.
First group of additive
Second step
Get chlorella dry algae powder 3.0g and be dissolved in the ethanol of 500ml95%, fully stir or shake up 60min.Be mainly the Alcohol soluble compositions such as extraction lipoprotein, β-carotene, chlorophyll a, alcohol soluble vitamin, gamma-linolenic acid, supernatant liquor is grass green.With the silk cover filtering of mesh aperture 80-100 μ m, remove after sediment, leave clear liquid; By the centrifugal 5min of whizzer for clear liquid, rotating speed 5000r/min, gets supernatant liquor and leaves standstill 60h, then get supernatant liquor, adds second group of additive (every 1000ml supernatant liquor addition is as follows), fully shakes up, and obtains B liquid, and 4 ℃ of Refrigerator stores are for subsequent use.
Figure BSA0000101570810000052
The 3rd step
Get wine base (or former wine) 500ml, add A liquid 30ml, fully stir or shake up 25min; Add again B liquid 20ml, fully shake up or stir 45min, now have a small amount of white flocks; With the silk cover filtering of mesh aperture 60-100 μ m, throw aside cotton-shaped dregs and foam, get the centrifugal 10min of clear liquid, rotating speed 5000r/min, gets supernatant liquor and leaves standstill 72h, throws aside throw out,
Get supernatant liquor, bottling completes.
Precaution
1) in second step, ethanol used should be the ethanol of the fermentative production such as grain, i.e. grain wine, but not industrial ethyl alcohol;
2) institute in steps, the particularly preparation process of A liquid, should not under high light (being no more than 8000lux) or direct sunlight and high temperature, (be no more than 35 ℃) carries out;
3) preparation process of A liquid should complete as far as possible in the short period of time, in order to avoid water-soluble nutritive ingredient sex change;
4) when finished wine is preserved, should avoid direct sunlight; Preferably 0-12 ℃ of shady and cool place ventilation lucifugal preserved;
5) two groups of additives all add by given order in formula, often add one and add lower one every 3min again, and need constantly to stir, and to avoid chemical reaction.
Need to illustrate, the numerical range in specific embodiment can realize, and more than end points and mid point can be realized, and length limit is not carried out exhaustive at this.
Finally it should be noted that: above embodiment only, in order to technical scheme of the present invention to be described, is not intended to limit; Although the present invention is had been described in detail with reference to previous embodiment, those of ordinary skill in the art is to be understood that: its technical scheme that still can record previous embodiment is modified, or part technical characterictic is wherein equal to replacement; And these modifications or replacement do not make the essence of appropriate technical solution depart from the spirit and scope of embodiment of the present invention technical scheme.

Claims (3)

1. a marine alga wine making method, is characterized in that this marine alga wine making step is as follows:
The first step, gets chlorella dry algae powder 2.0g and is dissolved in 500ml distilled water, fully stirs or shake up 45min, comes extraction water soluble vitamin, micro-polysaccharides and CGF active factor, and supernatant liquor is sky blue; With the silk cover filtering of mesh aperture 100 μ m, throw aside after sediment and foam, by the centrifugal 10min of whizzer for supernatant liquor, rotating speed 4500r/min, throws aside throw out, leaves supernatant liquor, leave standstill 48h, again throw aside sediment, get supernatant liquor and add first group of additive, wherein to add amount and the component of first group of additive as follows for every 1000ml supernatant liquor:
Figure FSA0000101570800000011
Then fully shake up, obtain A liquid, 4 ℃ of Refrigerator stores, for subsequent use;
Second step, gets chlorella dry algae powder 3.0g and is dissolved in the ethanol of 500ml95%, fully stirs or shake up 60min, extracts lipoprotein, β-carotene, chlorophyll a, alcohol soluble vitamin, these Alcohol soluble compositions of gamma-linolenic acid, and supernatant liquor is grass green; With the silk cover filtering of mesh aperture 80-100 μ m, remove after sediment, leave clear liquid; By whizzer centrifugal 5min for clear liquid, rotating speed 5000r/min, throws aside throw out, gets supernatant liquor and leaves standstill 60h, again throws aside sediment, gets supernatant liquor, adds second group of additive, and wherein to add amount and the component of second group of additive as follows for every 1000ml supernatant liquor:
Figure FSA0000101570800000021
Then fully shake up, obtain B liquid, 4 ℃ of Refrigerator stores, for subsequent use;
The 3rd step, gets wine base (or former wine) 500ml, adds A liquid 30ml, fully stirs or shake up 25min; Add again B liquid 20ml, fully shake up or stir 45min, now have a small amount of white flocks; With the silk cover filtering of mesh aperture 60-100 μ m, throw aside cotton-shaped dregs and foam, get the centrifugal 10min of clear liquid, rotating speed 5000r/min, throws aside throw out, gets supernatant liquor, leaves standstill 72h, again throws aside throw out, gets supernatant liquor, and bottling completes.
2. the additive using in a kind of marine alga wine making method as claimed in claim 1, is characterized in that this binder component and proportioning are as follows:
Figure FSA0000101570800000022
3. the additive using in a kind of marine alga wine making method as claimed in claim 1, is characterized in that this binder component and proportioning are as follows:
Figure FSA0000101570800000031
CN201410075136.9A 2014-03-04 2014-03-04 A kind of making method of marine alga wine Active CN103805487B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410075136.9A CN103805487B (en) 2014-03-04 2014-03-04 A kind of making method of marine alga wine

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410075136.9A CN103805487B (en) 2014-03-04 2014-03-04 A kind of making method of marine alga wine

Publications (2)

Publication Number Publication Date
CN103805487A true CN103805487A (en) 2014-05-21
CN103805487B CN103805487B (en) 2016-04-06

Family

ID=50702879

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410075136.9A Active CN103805487B (en) 2014-03-04 2014-03-04 A kind of making method of marine alga wine

Country Status (1)

Country Link
CN (1) CN103805487B (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104263603A (en) * 2014-10-20 2015-01-07 临沂大学 Wine with arctium lappa and dunaliella salina and preparation technology thereof
CN105567492A (en) * 2015-12-16 2016-05-11 苏州垣梦新能源环保科技有限公司 Nutritional wine prepared from composition of bacteria, algae and dendrobium nobile

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101810336A (en) * 2010-04-30 2010-08-25 广东仙乐制药有限公司 Chewable soft capsules and method for preparing same
CN102994332A (en) * 2012-08-23 2013-03-27 王培磊 Making method of spirulina wine

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101810336A (en) * 2010-04-30 2010-08-25 广东仙乐制药有限公司 Chewable soft capsules and method for preparing same
CN102994332A (en) * 2012-08-23 2013-03-27 王培磊 Making method of spirulina wine

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104263603A (en) * 2014-10-20 2015-01-07 临沂大学 Wine with arctium lappa and dunaliella salina and preparation technology thereof
CN104263603B (en) * 2014-10-20 2017-01-18 临沂大学 Wine with arctium lappa and dunaliella salina and preparation technology thereof
CN105567492A (en) * 2015-12-16 2016-05-11 苏州垣梦新能源环保科技有限公司 Nutritional wine prepared from composition of bacteria, algae and dendrobium nobile

Also Published As

Publication number Publication date
CN103805487B (en) 2016-04-06

Similar Documents

Publication Publication Date Title
Costa et al. Operational and economic aspects of Spirulina-based biorefinery
Hamed The evolution and versatility of microalgal biotechnology: A review
CN101352249B (en) Production method for fully using oil-containing micro-algae
CN102295983A (en) Production method for comprehensive utilization of microalgae
CN104757334A (en) Algin for fodder
CN103627514A (en) Method for extracting grape seed oil from grape seeds
CN102994332B (en) Making method of spirulina wine
CN103708921A (en) Lentinula edodes planting matrix material and treatment method thereof
CN103805487B (en) A kind of making method of marine alga wine
CN106070339A (en) Brown algae polyphenols compositions with algistatic activity and its preparation method and application
CN102260587A (en) Method for extracting grape seed oil and proantho cyanidins
Dibenedetto Production of aquatic biomass and extraction of bio-oil
CN109504609A (en) The cultural method of seaweed for zooplankter bait
CN104026683B (en) The method of persimmon fruit juice is prepared with persimmon pericarp
CN106119000A (en) A kind of chlorella rice wine and brewing method
CN102669420B (en) Functional type pre-mixed tea seed cake dreg feed and production method thereof
CN112481014A (en) Enzymatic extraction process for oil in camellia seed kernels
CN110004189A (en) A method of single needle algae oil and fat accumulation is improved based on fulvic acid joint salt stress
CN108276797B (en) Preparation method and application of natural edible pigment dunaliella salina green
Dibenedetto et al. Production and uses of aquatic biomass
Arias Calvo et al. Macroalgae biorefineries as a sustainable resource in the extraction of value-added compounds
da Silva Marinho Bioremediation capacity, nutritional value and biorefining of macroalga Saccharina latissima
CN106854490A (en) A kind of technology of Ultrasonic-microwave synergistic extraction algal oil
CN105803034A (en) Method for inducing freshwater chlorella pyrenoidosa (C. pyrenoidosa) ZF strain to efficiently accumulate carotenoids by utilizing plant growth regulator NAA
CN110604313A (en) Method for preparing high-protein high-calcium supplement by using mussel waste materials

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20210701

Address after: 223, xinaolin building, No.15, Gaoxin North 2nd Road, songpingshan community, Xili street, Nanshan District, Shenzhen, Guangdong 518000

Patentee after: Shenzhen space microalgae Biotechnology Co.,Ltd.

Address before: 276000 Linyi University, middle section of Shuangling Road, Linyi, Shandong Province

Patentee before: LINYI University