CN103804945B - Extraction method of black persimmon peel pigment - Google Patents
Extraction method of black persimmon peel pigment Download PDFInfo
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- CN103804945B CN103804945B CN201410033556.0A CN201410033556A CN103804945B CN 103804945 B CN103804945 B CN 103804945B CN 201410033556 A CN201410033556 A CN 201410033556A CN 103804945 B CN103804945 B CN 103804945B
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Abstract
The invention discloses an extraction method of a black persimmon peel pigment. The method comprises the following steps: harvesting black persimmon fruits with peels which become dark to obtain the peels; carrying out liquid nitrogen quick freezing or freezing drying on the peels, and sufficiently grinding the peels into powder; weighing the powdery black persimmon peel; adding acetone; sufficiently and uniformly mixing, and extracting; pouring off liquid supernatant; adding an HCl-methanol solution into a primary extractive; extracting and separating to obtain primary liquid supernatant and pigment extracting residual dregs; adding the HCl-methanol solution into the pigment extracting residual dregs; sufficiently and uniformly mixing, and extracting and separating to obtain secondary liquid supernatant; and combining the primary liquid supernatant and the secondary liquid supernatant to obtain a claret-colored black persimmon peel pigment extracting solution. The extraction method for the black persimmon peel pigment is simple in process, low in cost and high in extracting rate.
Description
Technical field
The present invention relates to a kind of extracting method of natural plant pigment, refer to a kind of extracting method of black persimmon pericarp pigment particularly.
Background technology
Medical science toxicologic study finds not only have no nutritive value for the chemosynthesis pigment of food, beverage in recent years, and part also has intoxicating effect (comprising toxicity, teratogenecity and carinogenicity).Along with people's living standard raising and health perception strengthen day by day, many synthetic food colors are just gradually by natural plant pigment is replaced.Natural pigment is many from resources such as fruit, vegetables, flowers, higher to the security of human body, be easily absorbed by the body, itself has nourishing function concurrently again, for cancer, heart trouble, ophthalmic diseases etc., there is good preventive effect, be widely used in the industries such as beverage, candy, cake, drinks and snackfoods.
China is the country of origin of persimmon (Diospyros kaki Thunb.), Food and Argriculture OrganizationFAO's statistics display in 2013, China's persimmon harvest area 720,000 hectares, and output 3,050,000 tons is the country that persimmon cultivated area is maximum and persimmon fruit output is the highest in the world.China's persimmon aboundresources, has more than 1000 kind, and wherein the persimmon fruit colour of more than 99% is yellow, and has a special kind, and when it is ripe, fruit colour is black, is called black persimmon.It is strong that black persimmon has growth adaptability, and disease and pest is few, and output is high, large and the feature that result is stable of result quantities, melanochrome in its pericarp is a kind of natural plant pigment, has important economic worth and good industrialization prospect with black persimmon for raw material is separated its pericarp natural pigment.At present also not about any report that black persimmon pericarp pigment extracts.
Summary of the invention
Technical problem to be solved by this invention is just to provide a kind of extracting method of black persimmon pericarp pigment.
For solving the problems of the technologies described above, the extracting method of a kind of black persimmon pericarp pigment provided by the invention, comprises the following steps:
1) to gather the black persimmon of pericarp blackening, remove pulp and obtain pericarp, abundant grind into powder by pericarp and after liquid nitrogen flash freezer or lyophilize;
2) take pulverous black persimmon pericarp in step 1), by the acetone adding 100 ~ 150mL in every gram of black persimmon pericarp, fully mixing is placed in water-bath and extracts;
3) rotating centrifugal, outwells supernatant liquor, adds HCl-methanol solution in primary extract, and fully mixing is placed in 60 ~ 65 DEG C of water-baths and extracts 1 ~ 2h, wherein, takes the HCl-methanol solution of 15 ~ 25mL by every gram of black persimmon pericarp;
4) rotating centrifugal again, be separated first time supernatant liquor and pigment extract residue, under supernatant liquor being placed in 4 DEG C of conditions;
5) pigment obtained to step 4) extracts in residue and adds HCl-methanol solution, and fully mixing is placed in 60 ~ 65 DEG C of water-baths and extracts 1 ~ 2h; Rotating centrifugal is separated and obtains second time supernatant liquor, wherein, takes the HCl-methanol solution of 15 ~ 25mL by every gram of black persimmon pericarp;
6) step 4) is obtained first time supernatant liquor and step 5) obtain second time supernatant liquor merge, namely obtain claret-colored black persimmon pericarp pigment extracting solution.
Further, described step 2) in, bath temperature is 30 ~ 35 DEG C, and extraction time is 1 ~ 2h
Again further, in described step 3), rotating speed is 3000 ~ 8000rpm, and centrifugation time is 10 ~ 20min.
Again further, in described step 4), rotating speed is 3000 ~ 8000rpm, and centrifugation time is 10 ~ 20min.
Again further, in described step 5), rotating speed is 3000 ~ 8000rpm, and centrifugation time is 10 ~ 20min.
Again further, in described step 5), the massfraction of HCl-methanol solution is 5%.
Beneficial effect of the present invention is:
Present invention process is simple, and with low cost, extraction yield is high.
Embodiment
In order to explain the present invention better, illustrate main contents of the present invention further below in conjunction with specific embodiment, but content of the present invention is not only confined to following examples.
Embodiment 1
An extracting method for black persimmon pericarp pigment, comprises the following steps:
1) to gather the black persimmon fruit of pericarp blackening, obtain pericarp and after liquid nitrogen flash freezer or lyophilize abundant grind into powder;
2) take pulverous black persimmon pericarp in 0.3g step 1), add the acetone of 30mL, fully mixing is placed in 30 DEG C of thermostat water baths and extracts 1h;
3) centrifugal 15min under 3000 ~ 8000rpm condition, outwells supernatant liquor, adds the HCl-methanol solution that 5mL massfraction is 5% in primary extract, and fully mixing is placed in 60 DEG C of thermostat water baths and extracts 1h;
4) centrifugal 15min under 3000 ~ 8000rpm condition, is separated supernatant liquor and pigment for the first time and extracts residue, under supernatant liquor being placed in 4 DEG C of conditions;
5) pigment obtained to step 4) extracts in residue and adds the HCl-methanol solution that 5mL massfraction is 5%, and fully mixing is placed in 60 DEG C of water-baths and extracts 1h; Centrifugal 15min under 3000 ~ 8000rpm condition, is separated and obtains second time supernatant liquor,
6) step 4) is obtained first time supernatant liquor and step 5) obtain second time supernatant liquor merge, namely obtain claret-colored black persimmon pericarp pigment extracting solution.
Embodiment 2
An extracting method for black persimmon pericarp pigment, comprises the following steps:
1) to gather the black persimmon fruit of pericarp blackening, obtain pericarp and after liquid nitrogen flash freezer or lyophilize abundant grind into powder;
2) take pulverous black persimmon pericarp in 0.3g step 1), add the acetone of 45mL, fully mixing is placed in 35 DEG C of thermostat water baths and extracts 2h;
3) centrifugal 10min under 3000 ~ 8000rpm condition, outwells supernatant liquor, adds the HCl-methanol solution that 4.5mL massfraction is 5% in primary extract, and fully mixing is placed in 65 DEG C of thermostat water baths and extracts 1h;
4) centrifugal 10min under 3000 ~ 8000rpm condition, is separated supernatant liquor and pigment for the first time and extracts residue, under supernatant liquor being placed in 4 DEG C of conditions;
5) pigment obtained to step 4) extracts in residue and adds the HCl-methanol solution that 4.5mL massfraction is 5%, and fully mixing is placed in 65 DEG C of water-baths and extracts 1h; Centrifugal 10min under 3000 ~ 8000rpm condition, is separated and obtains second time supernatant liquor,
6) step 4) is obtained first time supernatant liquor and step 5) obtain second time supernatant liquor merge, namely obtain claret-colored black persimmon pericarp pigment extracting solution.
Embodiment 3
An extracting method for black persimmon pericarp pigment, comprises the following steps:
1) to gather the black persimmon fruit of pericarp blackening, obtain pericarp and after liquid nitrogen flash freezer or lyophilize abundant grind into powder;
2) take pulverous black persimmon pericarp in 0.3g step 1), add the acetone of 40mL, fully mixing is placed in 30 DEG C of thermostat water baths and extracts 2h;
3) centrifugal 20min under 3000 ~ 8000rpm condition, outwells supernatant liquor, adds the HCl-methanol solution that 7.5mL massfraction is 5% in primary extract, and fully mixing is placed in 60 DEG C of thermostat water baths and extracts 1h;
4) centrifugal 20min under 3000 ~ 8000rpm condition, is separated supernatant liquor and pigment for the first time and extracts residue, under supernatant liquor being placed in 4 DEG C of conditions;
5) pigment obtained to step 4) extracts in residue and adds the HCl-methanol solution that 7.5mL massfraction is 5%, and fully mixing is placed in 60 DEG C of water-baths and extracts 1h; Centrifugal 20min under 3000 ~ 8000rpm condition, is separated and obtains second time supernatant liquor,
6) step 4) is obtained first time supernatant liquor and step 5) obtain second time supernatant liquor merge, namely obtain claret-colored black persimmon pericarp pigment extracting solution.
Other unspecified part is prior art.Although above-described embodiment is to invention has been detailed description; but it is only the present invention's part embodiment; instead of whole embodiment, people can also obtain other embodiments according to the present embodiment under without creative prerequisite, and these embodiments all belong to scope.
Claims (6)
1. an extracting method for black persimmon pericarp pigment, is characterized in that: comprise the following steps:
1) to gather the black persimmon of pericarp blackening, remove pulp and obtain pericarp, by pericarp abundant grind into powder after liquid nitrogen flash freezer;
2) take step 1) in pulverous black persimmon pericarp, by the acetone adding 100 ~ 150mL in every gram of black persimmon pericarp, fully mixing is placed in water-bath and extracts;
3) rotating centrifugal, outwells supernatant liquor, adds HCl-methanol solution in primary extract, and fully mixing is placed in 60 ~ 65 DEG C of water-baths and extracts 1 ~ 2h, wherein, takes the HCl-methanol solution of 15 ~ 25mL by every gram of black persimmon pericarp;
4) rotating centrifugal again, be separated first time supernatant liquor and pigment extract residue, under supernatant liquor being placed in 4 DEG C of conditions;
5) to step 4) pigment that obtains extracts in residue and adds HCl-methanol solution, and fully mixing is placed in 60 ~ 65 DEG C of water-baths and extracts 1 ~ 2h; Rotating centrifugal is separated and obtains second time supernatant liquor, wherein, takes the HCl-methanol solution of 15 ~ 25mL by every gram of black persimmon pericarp;
6) by step 4) obtain first time supernatant liquor and step 5) obtain second time supernatant liquor merge, namely obtain claret-colored black persimmon pericarp pigment extracting solution.
2. the extracting method of black persimmon pericarp pigment according to claim 1, is characterized in that: described step 2) in, bath temperature is 30 ~ 35 DEG C, and extraction time is 1 ~ 2h.
3. the extracting method of black persimmon pericarp pigment according to claim 1 and 2, is characterized in that: described step 3) in, rotating speed is 3000 ~ 8000rpm, and centrifugation time is 10 ~ 20min.
4. the extracting method of black persimmon pericarp pigment according to claim 1 and 2, is characterized in that: described step 4) in, rotating speed is 3000 ~ 8000rpm, and centrifugation time is 10 ~ 20min.
5. the extracting method of black persimmon pericarp pigment according to claim 1 and 2, is characterized in that: described step 5) in, rotating speed is 3000 ~ 8000rpm, and centrifugation time is 10 ~ 20min.
6. the extracting method of black persimmon pericarp pigment according to claim 1 and 2, is characterized in that: described step 5) in, the massfraction of HCl-methanol solution is 5%.
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| CN104559304B (en) * | 2014-12-19 | 2017-06-23 | 桂林得坤生物科技股份有限公司 | A kind of extracting method of persimmon fruit pigment |
| CN104693834B (en) * | 2015-03-31 | 2017-05-17 | 桂林得坤生物科技股份有限公司 | Method for preparing persimmon fruit pigments |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1786081A (en) * | 2004-12-10 | 2006-06-14 | 西北工业大学 | Method of extracting natural yellow pigment from persimon leaf or persion bark |
| CN102321982A (en) * | 2011-08-10 | 2012-01-18 | 无锡市联盛印染有限公司 | Dyeing method by using persimmon natural dye |
| CN102399451A (en) * | 2010-09-08 | 2012-04-04 | 苗广义 | Method for extracting pigment from persimmon peel |
| CN103013164A (en) * | 2012-12-14 | 2013-04-03 | 桂林普兰德生物科技有限公司 | Method for extracting natural pigment from persimmon peels |
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2014
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1786081A (en) * | 2004-12-10 | 2006-06-14 | 西北工业大学 | Method of extracting natural yellow pigment from persimon leaf or persion bark |
| CN102399451A (en) * | 2010-09-08 | 2012-04-04 | 苗广义 | Method for extracting pigment from persimmon peel |
| CN102321982A (en) * | 2011-08-10 | 2012-01-18 | 无锡市联盛印染有限公司 | Dyeing method by using persimmon natural dye |
| CN103013164A (en) * | 2012-12-14 | 2013-04-03 | 桂林普兰德生物科技有限公司 | Method for extracting natural pigment from persimmon peels |
Non-Patent Citations (3)
| Title |
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| "柿色素的提取及稳定性研究";张雅利 等;《食品科技》;20061231(第10期);148-152 * |
| "涉县柿树品种调查";崔怀仙;《安徽农学通报》;20091231;第15卷(第8期);130-131 * |
| 陈栓虎 等."柿子红色素的提取及稳定性研究".《西北大学学报(自然科学版)》.2004,第34卷(第6期),677-679. * |
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