CN103800559B - External medicine composition, preparation with white-skinned face function of releiving and preparation method thereof - Google Patents
External medicine composition, preparation with white-skinned face function of releiving and preparation method thereof Download PDFInfo
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- CN103800559B CN103800559B CN201410018343.0A CN201410018343A CN103800559B CN 103800559 B CN103800559 B CN 103800559B CN 201410018343 A CN201410018343 A CN 201410018343A CN 103800559 B CN103800559 B CN 103800559B
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Abstract
The present invention provides a kind of external medicine composition with white-skinned face function of releiving, preparation and preparation method thereof.The present invention is based on China's Traditional Chinese Medicine theory, and skin health is objective, using composite traditional Chinese medicine extract as main functional additive, is aided with cosmetics conventional matrix components and is formulated.This external medicine composition and its preparation have releive, white-skinned face function, its composite traditional Chinese medicine extract natural green is healthy and safe, can the extensive use in effect cosmetic of releiving.
Description
Technical field
The present invention relates to a kind of external medicine composition, specifically a kind of external application Chinese medicine with white-skinned face function of releiving
Composition and the Chinese medicine preparation prepared by the skin care compositions.
Background technology
Allergic is a very common physiological reaction.Most of people can have allergic experience.With
Modern society continues to develop, and what cosmetics, chemicals were used is continuously increased, increasing environmental pollution and pollen,
The original sensitizer such as some foods is all continuously increased allergic possibility.Due to a variety of causes so that sensitiveness skin
The crowd of skin is also continuously increased.Sensitiveness skin Dermatosis incidence research to various countries shows that sensitive has higher hair
Raw rate.
It is reported that European, the women of the U.S. and Japan about 40%~50% is sensitiveness skin.Britain is tested to 2058
Object（Including masculinity and femininity）Research in find, there is asking for skin sensitivity in about 51.4% women and 38.2% male
Topic.The people that Joudain R etc. carry out in telephone poll, surveyee 52% to not agnate 800 American Women's shows oneself
For sensitive.France carries out telephone poll to more than 15 years old crowd, and 52% surveyee is sensitive, wherein greatly
Majority is women, and the ratio of masculinity and femininity sensitive subjects is 44% and 59% respectively.And in China, a research table of L'Oreal
Bright, about 36.1% women is sensitive.
On current Cosmetic Market, all skin care brands are released for sensitive skin in addition to indivedual color make-up brands
Product, and number amount and type increasing, show sensitive skin market just by manufacturer most attention and before its market
Scape is wide.
So in summary, it is with real meaning and wide to develop a cosmetics with outstanding effect of releiving
Market prospects.
The content of the invention
The primary and foremost purpose of the present invention is to propose a kind of Chinese medicine composition with white-skinned face function of releiving.
Another object of the present invention is to propose a kind of preparation method of above-mentioned Chinese medicine composition.
The present invention invention thinking be：
The present invention utilizes advanced biotechnology, with reference to the theory of traditional Chinese medical science of Chinese tradition, by oldenlandia diffusa, the root bark of Chinese wolf-berry,
Cactus, kuh-seng, ginkgo leaf, Radix Glycyrrhizae and duck wheat are effectively combined in together, form compound, and as active component,
Skin care formulation is prepared into, it has white-skinned face function of releiving, so as to effectively prevention and different types of allergic phenomena of releiving.
Oldenlandia diffusa：It is patent medicine bitter, light, it is cold in nature.Primary efficacy is clearing heat and detoxicating, medicinal powder for relieving pain knot, promoting urination and removing dampness.It is outstanding kind
Treat all kinds inflammation.
The root bark of Chinese wolf-berry：It is cold in nature, it is sweet.Major function：Cool blood is except steaming, clearing lung-heat fall fire.For deficiency of Yin hectic fever, hectic fever due to yin night sweat, lung
Heat cough, spitting of blood, bleeding from five sense organs or subcutaneous tissue.
Cactus：It is cold in nature, it is bitter, puckery.Enter the heart, lung, the warp of stomach three.With clearing heat and detoxicating, stimulate the circulation of the blood and cause the muscles and joints to relax, eliminating stasis to subdue swelling, solution
Intestines poison, cooling blood and relieving pain, ease constipation hemostasis, benefiting stomach and stopping pain, effect of antibechic.
Kuh-seng：Bitter；It is cold in nature.Return liver；Kidney；Large intestine；Small intestinl channel；Bladder；The heart channel of Hang-Shaoyin；Bladder warp.With heat-clearing and damp-drying drug, kill
Worm, diuresis effect.
Ginkgo leaf：Nature and flavor are sweet, bitter, puckery, put down.The thoughts of returning home, lung channel.With astringing the lung, relieving asthma, promoting blood circulation and removing blood stasis, effect of analgesic.
Radix Glycyrrhizae：Nature and flavor are sweet, put down.Enter spleen, stomach, lung channel.Major function is clearing heat and detoxicating, expelling phlegm and arresting coughing, gastral cavity abdomen etc.
Duck wheat：It is bitter in taste, flat, cold, there are benefiting energy, continuous spirit, sharp knowledge, the wide intestines stomach invigorating of sending down abnormally ascending.
To realize the purpose of the present invention, the present invention is adopted the following technical scheme that：
A kind of external medicine composition with white-skinned face function of releiving, the bulk drug matched by following weight parts is made：
It is oldenlandia diffusa 10~30, the root bark of Chinese wolf-berry 10~30, cactus 10~20, kuh-seng 10~20, ginkgo leaf 10~20, sweet
Grass 10~20, duck wheat 10~20.
In the external medicine composition, each preferable weight of bulk drug is：
It is oldenlandia diffusa 20~30, the root bark of Chinese wolf-berry 20~30, cactus 15~20, kuh-seng 15~20, ginkgo leaf 10~15, sweet
Grass 10~15, duck wheat 10~15.
A kind of preparation method of above-mentioned external medicine composition, comprises the following steps：
（1）Bulk drug is weighed according to above-mentioned weight；
（2）Percent by volume is that 75%~80% ethanol is extracted twice, and bulk drug and ethanol mass volume ratio are extracted for the first time
G/ml is 1：10~1：20,60 DEG C~70 DEG C heating stirrings 1~2 hour, filter to obtain first time filtrate；Second of extraction bulk drug
It is 1 with ethanol mass volume ratio g/ml：10~1：20,60 DEG C~70 DEG C heating stirring 30min~60min, are filtered to obtain second
Filtrate；First time filtrate and second of filtrate mixing, are concentrated into 0.2~0.5g/ml, obtain concentrate；
（3）It is 1 according to mass ratio：1~1：2 to step（2）Propane diols or butanediol are added in obtained concentrate, is taken out
Filter, 3000~5000 revs/min of centrifuging and taking supernatants are produced.
Wherein more preferably, the step（2）It is 1 that middle first time, which extracts bulk drug with ethanol mass volume ratio g/ml,：20；60
DEG C heating stirring 1 hour.
Wherein more preferably, the step（2）In second to extract bulk drug with ethanol mass volume ratio g/ml be 1：10；60
DEG C heating stirring 30 minutes.
A kind of external application Chinese medicine extract with white-skinned face function of releiving, is extracted by the above method and obtained.
Above-mentioned external application Chinese medicine extract is being prepared with the purposes in the skin care formulation for releiving white-skinned face function.
A kind of skin care formulation with white-skinned face function of releiving, the skin care formulation is by above-mentioned external application Chinese medicine extract and skin care
Product field customary adjuvant is made.
A kind of external skin-care composition with white-skinned face function of releiving, the external skin-care composition includes A phase B phases, C phases
With D phases, A, B, C, D phase is made up of the raw material of following weight percentage：
A kind of preparation method of above-mentioned external skin-care composition, comprises the following steps：
（1）Double-PEG-18 methyl ethers dimethylsilanes first are heated into 40 DEG C~50 DEG C with water to be completely dissolved, it is stand-by；
（2）A phases：Water is added in aqueous phase tank after weighing up, by acrylic acid（Ester）Class/C10-30 alkylol acrylamide acid esters cross-linked polymerics
Thing is uniformly sprinkled upon water surface, and after after immersion completely, other raw materials of addition A phases stir 30~50 revs/min, stirred；
（3）By step（2）The A phases prepared are pumped into emulsion tank, and vacuum is mixed, and 30~50 turns of stir speed (S.S.)/
Minute, it is heated to 80~85 DEG C of 30~40min of insulation, stirring cooling；Rate of temperature fall is 1~2 DEG C/min；
（4）When temperature drops to 45~50 DEG C, B phase NaOH are added, are stirred under vacuum uniform；
（5）When temperature is dropped to below 40 DEG C, C phases and D phase raw materials are added, is stirred under vacuum uniformly, produces.
The preparation that above-mentioned raw materials and method are prepared is Essence.Utilize the Chinese medicine composition and skin care item in the present invention
Field conventional method and auxiliary material can also prepare other formulations, such as face cream, toner, emulsion, spray.In the present invention
Chinese medicine composition be simply pulverized and mixed and may also function as above-mentioned effect.
Essence of the present invention, it is recommended to use method is：After daily face cleaning, Essence is coated on skin, gently pressed
Rub, treat that Essence slowly absorbs.
The present invention is verified by experiments non-stimulated to skin safe, and Chinese medical extract not only has in itself releives effect, also has
There is white-skinned face function.The raw material of the present invention are obtained easy, preparation method simplicity, green safety, it is easy to received by allergic human population, tool
There are good application prospect and market prospects.
Brief description of the drawings
Fig. 1 is measurement result of the Chinese medical extract to hyaluronic acid enzyme inhibition rate；
Fig. 2 is that Chinese medicine extract is tested to the inhibiting rate of tyrosinase.
Embodiment
Medicinal material used of the invention can be commercially available from China Medicament Group Corp., meet Pharmacopoeia of People's Republic of China
2005 editions standards.Other raw materials can be obtained by commercially available purchase, and the present invention is shown in Table 1 using the source of raw material, and the present invention is used
Instrument title and producer be shown in Table 2.
Table 1
| Title | Model | Producer |
| Ultraviolet-uisible spectrophotometer | UV2300 | Beijing Pu Xi all purpose instruments Co., Ltd |
| Ultrasonic wave circulates extractor | SCIENTZ-HF5000 | Henan brother's experimental instruments and equipment limited |
| Dermal melanin and ferroheme tester | Mexameter MX18 | German CK electronics corporations |
| Refrigerated centrifuge | 4K15 | Sigma companies |
| Homogenizer | BMS602 | BRT companies |
Table 2
The preparation of the Chinese medicine composition of the present invention of embodiment 1
（1）Bulk drug is weighed according to following weight parts proportioning；
Oldenlandia diffusa 30g, root bark of Chinese wolf-berry 20g, cactus 10g, kuh-seng 20g, ginkgo leaf 10g, Radix Glycyrrhizae 15g, duck wheat
10g。
（2）Percent by volume is that 75% ethanol is extracted twice, and bulk drug is extracted for the first time is with ethanol mass volume ratio g/ml
1：10,60 DEG C of heating stirrings 1 hour, filter to obtain first time filtrate；Second of extraction bulk drug and ethanol mass volume ratio g/ml
For 1：10,60 DEG C of heating stirring 30min, filter to obtain second of filtrate；First time filtrate and second of filtrate mixing, rotary evaporation
0.2g/ml is concentrated into, concentrate is obtained；
（3）It is 1 according to mass ratio：2 to step（2）Add propane diols in obtained concentrate to redissolve, suction filtration, 3000 turns/
Minute centrifuging and taking supernatant, is produced.
The preparation of the Chinese medicine composition of the present invention of embodiment 2
（1）Bulk drug is weighed according to following weight parts proportioning；
Oldenlandia diffusa 20g, root bark of Chinese wolf-berry 30g, cactus 20g, kuh-seng 10g, ginkgo leaf 20g, licorice 20g, duck wheat
15g。
（2）Percent by volume is that 80% ethanol is extracted twice, and bulk drug is extracted for the first time is with ethanol mass volume ratio g/ml
1：20,70 DEG C of heating stirrings 2 hours, filter to obtain first time filtrate；Second of extraction bulk drug and ethanol mass volume ratio g/ml
For 1：20,70 DEG C of heating stirring 60min, filter to obtain second of filtrate；First time filtrate and second of filtrate mixing, rotary evaporation
0.3g/ml is concentrated into, concentrate is obtained；
（3）It is 1 according to mass ratio：1 to step（2）Butanediol, suction filtration, 5000 revs/min are added in obtained concentrate
Centrifuging and taking supernatant, collects filtrate, produces.
The preparation of the Chinese medicine composition of the present invention of embodiment 3
（1）Bulk drug is weighed according to following weight parts proportioning；
Oldenlandia diffusa 10g, root bark of Chinese wolf-berry 10g, cactus 15g, kuh-seng 15g, ginkgo leaf 15g, licorice 10g, duck wheat
20g。
（2）Percent by volume is that 75% ethanol is extracted twice, and bulk drug is extracted for the first time is with ethanol mass volume ratio g/ml
1：15,65 DEG C of heating stirrings 1.5 hours, filter to obtain first time filtrate；Second of extraction bulk drug and ethanol mass volume ratio g/
Ml is 1：15,65 DEG C of heating stirring 40min, filter to obtain second of filtrate；First time filtrate and second of filtrate mixing, rotation are steamed
Hair is concentrated into 0.5g/ml, obtains concentrate；
（3）It is 1 according to mass ratio：1.5 to step（2）Butanediol, suction filtration, 4000 revs/min are added in obtained concentrate
Clock centrifuging and taking supernatant, is produced.
The preparation of the Essence of the present invention of embodiment 4
Raw material and proportioning（Mass percent）, it is shown in Table 3：
Table 3
Elite liquid and preparation method thereof：
（1）Double-PEG-18 methyl ethers dimethylsilanes first are heated into 40 DEG C with water to be completely dissolved, it is stand-by；
（2）A phases：Water is added in aqueous phase tank after weighing up, by acrylic acid（Ester）Class/C10-30 alkylol acrylamide acid esters cross-linked polymerics
Thing is uniformly sprinkled upon water surface（It is careful not to be spread across in pot wall）, after after immersion completely, other raw materials of addition A phases stir 30 revs/min
Clock, stirs；
（3）By step（2）The A phases prepared are pumped into emulsion tank, and vacuum mixes 30 revs/min, is heated to 80
DEG C insulation 30min, stir 30 revs/min cooling；Rate of temperature fall is 1 DEG C/min；
（4）When temperature drops to 45 DEG C, B phase NaOH are added, 30 revs/min is stirred under vacuum, stirs；
（5）When temperature is dropped to below 40 DEG C, C phases and D phase raw materials are added, 30 revs/min is stirred under vacuum, stirs,
Produce.
The preparation of the Essence of the present invention of embodiment 5
Raw material and proportioning（Mass percent）, it is shown in Table 4：
Table 4
Elite liquid and preparation method thereof：
（1）Double-PEG-18 methyl ethers dimethylsilanes first are heated into 50 DEG C with water to be completely dissolved, it is stand-by；
（2）A phases：Water is added in aqueous phase tank after weighing up, by acrylic acid（Ester）Class/C10-30 alkylol acrylamide acid esters cross-linked polymerics
Thing is uniformly sprinkled upon water surface（It is careful not to be spread across in pot wall）, after after immersion completely, other raw materials of addition A phases stir 50 revs/min
Clock, stirs；
（3）By step（2）The A phases prepared are pumped into emulsion tank, and vacuum mixes 50 revs/min, is heated to 85
DEG C insulation 40min, stir 30 revs/min cooling；Rate of temperature fall is 2 DEG C/min；
（4）When temperature drops to 50 DEG C, B phase NaOH are added, 50 revs/min is stirred under vacuum, stirs；
（5）When temperature is dropped to below 40 DEG C, C phases and D phase raw materials are added, 50 revs/min is stirred under vacuum, stirs,
Produce.
The preparation of the Essence of the present invention of embodiment 6
Raw material and proportioning（Mass percent）, it is shown in Table 5：
Table 5
Elite liquid and preparation method thereof：
（1）Double-PEG-18 methyl ethers dimethylsilanes first are heated into 45 DEG C with water to be completely dissolved, it is stand-by；
（2）A phases：Water is added in aqueous phase tank after weighing up, by acrylic acid（Ester）Class/C10-30 alkylol acrylamide acid esters cross-linked polymerics
Thing is uniformly sprinkled upon water surface（It is careful not to be spread across in pot wall）, after after immersion completely, other raw materials of addition A phases stir 40 revs/min
Clock, stirs；
（3）By step（2）The A phases prepared are pumped into emulsion tank, and vacuum mixes 40 revs/min, is heated to 85
DEG C insulation 35min, stir 40 revs/min cooling；Rate of temperature fall is 1 DEG C/min；
（4）When temperature drops to 50 DEG C, B phase NaOH are added, 40 revs/min is stirred under vacuum, stirs；
（5）When temperature is dropped to below 40 DEG C, C phases and D phase raw materials are added, 40 revs/min is stirred under vacuum, stirs,
Produce.
Efficacy experiments of the present invention
First, antianaphylaxis effect
1st, experimental principle
This method suppresses antiallergy effect that method determines cosmetic material and cosmetic product in vitro using hyaluronidase.
Hyaluronidase is the hyaluronic acid in anaphylactoid participant, decomposer（HA）, the acid thorn for making it become low molecule amount
Stimulus, causes histamine release, and induction body produces condition susceptible, and research shows that hyaluronidase and inflammation, allergy have strong correlation
Property, many Claritins play the role of high inhibition hyaluronidase activity.
Hyaluronidase can hydrolyze β-N-acetyl-glucosamine and D- glucuronates in hyaluronic acid indiscriminately
Between Isosorbide-5-Nitrae-key, and obtain β-N-acetyl-glucosamine, β-N-acetyl-glucosamine can contract with acetylacetone,2,4-pentanedione in the basic conditions
Symphysis adds lustre to former and p- dimethylaminobenzaldehyde in concentrated hydrochloric acid ethanol into the former 2- methyl -3- diacetyl azole derivatives that add lustre to
Add lustre to.
2nd, experiment content
Carried out using hyaluronidase body outer suppressioning experiment Elson-Morgan methods.Take 0.1mL0.25mmol/LCaCl2It is molten
37 DEG C of heat insulating culture 20min of liquid and 0.5mL hyaluronic acids enzyme liquid；Sample liquid 0.5mL is added, continues 37 DEG C of heat insulating culture 20min；
Add 0.5mL Sodium Hyaluronates liquid 37 DEG C of insulations 30min, room temperature 5min；Add 0.1mL0.4mol/LNaOH solution and
0.5mL acetylacetone,2,4-pentanedione solution, is placed in after heating 15min in boiling water bath and carries out cooling 5min with frozen water immediately；Add Ehrlich
Reagent 1.0mL is simultaneously diluted with 3.0mL absolute ethyl alcohols, 20min colour developings is placed, with its absorbance of spectrophotometric determination.
Antiallergic activity computing formula：
In formula：A --- contrast solution ABS values（Sample solution is replaced with hac buffer）
B --- control blank solution ABS values（Sample solution and enzyme liquid are replaced with hac buffer）
C --- sample solution ABS values
D --- sample blank solution A BS values（Enzyme liquid is replaced with hac buffer）
The length scanning of 450-700nm scopes is first carried out during experiment to A groups sample, to determine maximum absorption wavelength, then
Using deionized water as reference, ABS values measure is carried out respectively at the maximum absorption wavelength.
The difference of the measured value twice of same sample should be no more than the 10% of measure average value twice.
Sample is the Chinese medical extract of embodiment 1.
3rd, interpretation of result
Experiment uses the measurement result of the hyaluronic acid enzyme inhibition rate of sample as shown in figure 1, test concentrations is 1.0%
The aqueous solution, data are three average values.Analysis：Wherein positive control is antiallergic antipruritic.Inhibiting rate is having as seen from Figure 1
In the range of effect, the inhibiting rate of sample is in effective range（0-100%）, that is, have effects that to suppress hyaluronidase, and it is bigger,
Effect is better（Effective range 0-100%）.Therefore, the Chinese medical extract prepared in embodiment 1 has antiallergic effect.Inventor uses
Essence prepared by Chinese medical extract and embodiment 4,5,6 prepared by embodiment 2,3 repeats above-mentioned experimental result with above-mentioned.
2nd, tyrosinase Inhibition test
In the forming process of melanin, tyrosinase has played key enzyme, melanin produce number and tyrosine
The activity of enzyme has direct relation.Therefore, skin-whitening agents are exactly to be generated by acting on dermal melanin, in metabolic process,
Suppress melanin generation and meet the material of safety standard.It is anti-that with its substrate L-tyrosine catalysis can occur for L-tyrosinase
Should.After the reagent of L-tyrosinase activity inhibitory action is with the addition of in experimental system, suppression can be produced to catalytic reaction
Make and use, by the absorption photometric before and after measure addition reagent at 475nm, to evaluate suppression of the reagent to L-tyrosinase activity
Rate processed.
Solution is prepared needed for experiment：
a：Accurately weigh 17.91g disodium hydrogen phosphates to be dissolved in distilled water, use 500mL volumetric flask constant volumes；
b：Accurately weigh 7.8g sodium dihydrogen phosphate dihydrates to be dissolved in distilled water, use 500mL volumetric flask constant volumes；
c：Solution 107.4mL in solution 92.6mL in a, b is taken, 200mLpH=6.8PBS cushioning liquid is made into.
d：0.05%L-tyrosine solution：0.05gL- tyrosine accurately is weighed, first with a small amount of 0.1mol/L dissolving with hydrochloric acid,
After the phosphate buffer solution that pH=6.8 is used after its dissolving（PBS）It is settled to 100mL.
The white-skinned face function test experience of table 6 is with tabulation
Note：C1 and T1 adds 0.5mL tyrosinases, and enzyme activity is 100U/mL.
Experimental procedure：
（1）C2 pipes are prepared shake up after, the heating water bath 10min in 37 DEG C of water-baths returns to zero under wavelength 475nm.
（2）C1 pipe solution, which is prepared, to be shaken up, after 37 DEG C of water-bath 10min, plus tyrosinase 0.5ml, continues water-bath 10 minutes, is surveyed
Determine C1 absorbances.
（3）Press（1）（2）Same method, T1 absorbances are determined with T2 zeroings.
（4）Calculate maximum inhibition T of the sample to tyrosinase（%）.
Formula：T（%）=（C1-T1）/C1×100%
Sample uses the Chinese medical extract prepared in embodiment 1.
The result of tyrosinase Inhibition test is shown in Fig. 2, and embodiment 1 prepares extract with the increase of addition concentration, suppression
Rate processed is gradually stepped up, it was demonstrated that the extract has white-skinned face function.Extract is gradually carried with the increase of addition concentration, inhibiting rate
It is high, it was demonstrated that the extract has white-skinned face function.It is prepared by the Chinese medical extract and embodiment 4,5,6 that inventor is prepared with embodiment 2,3
Essence repeat above-mentioned experimental result with above-mentioned.
3rd, itching-relieving efficacies are studied --- and histamine phosphate causes method of itching
1st, experiment material
Experimental animal：Cavy 96, male and female half and half, 350 ± 20g.
Given the test agent：The Essence high dose group of embodiment 4 is 0.8g；Middle dose group is 0.4g；Low dose group is 0.2g.
Positive control：Fluocinonide ointment（1.0g/Kg）.
2nd, experiment reagent
The major experimental reagent summary sheet of table 7
3rd, laboratory apparatus
The major experimental instrument summary sheet of table 8
4th, experimental method
A. cavy 32 is taken, in testing instep shaving behind preceding 1 day right side, 4 groups are randomly divided into.I.e. sample sets, positive controls,
Distilled water negative control group, Normal group.
B. corresponding dosage sample, positive reference substance and distilled water, normal control are uniformly smeared respectively at shaving within continuous 2 days
Group is left intact.
C. test the 3rd day, it is appropriate that precision weighs histamine phosphate, be made into 0.01% before use with distilled water, 0.02%, 0.03%,
0.04% ... gradient concentration is standby.It will be abraded with coarse sandpaper at instep shaving behind the cavy right side, area about 1cm2, local coating 1 again
It is secondary, in dripping 0.01% histamine phosphate 0.05mL in abrasion after last coating 10min, later every 3 minutes according to 0.01%, 0.02%,
0.03%, 0.04% ... progressive concentration is 0.05mL every time.Turn one's head to lick right metapedes until there is cavy, finally to there is globefish
Mouse drips the histamine phosphate total amount taken when turning one's head to lick right metapedes be itch-threshold.
D. the otherness between each group itch-threshold and comparative group is calculated.
Sample uses Essence prepared by embodiment 4.
The Essence of table 9 causes the influence (x ± s, n=6) of reaction of itching to cavy histamine phosphate
Note：Compared with model control group, * p<0.05, * * p<0.01
As seen from the above table, compared with model group, FA ointment causes the reaction of itching to have extremely significant to cavy histamine phosphate
Inhibitory action（P<0.01）, sample sets are also with significant inhibitory action（P<0.05）.The essence that inventor is prepared with embodiment 5,6
Magnificent liquid repeats above-mentioned experimental result with above-mentioned.
4th, repair of the Essence to skin
A. cavy 32 is taken, 4 groups are randomly divided into.That is sample sets, positive controls, distilled water negative control group, normal right
According to group.
B. in l days before experiment, 6 groups of animals are anesthetized with ether, nape part hair is cut, area is 2 × 2cm2。
C. acetone is taken：Ether=1：The μ L drops of 1 mixed liquor 150 then take corresponding sample, positive control respectively at unhairing
Product and the μ L drops of distilled water 150 are at cropping, and 2 times/day, Normal group is left intact.
D. transepidermal water loss rate at its depilation was measured in the 5th day, compares the otherness of each group.
Sample uses Essence prepared by embodiment 4.
Repair of the table 10 to skin after allergy
Note：Compared with model control group, * p<0.05, * * p<0.01
As seen from the above table, compared with model group, sample sets have extremely significant repair to allergic skin（P<0.01）,
FA ointment has significant repair（P<0.05）.The Essence that inventor is prepared with embodiment 5,6 repeats above-mentioned experiment
As a result with above-mentioned.
Claims (10)
1. a kind of external medicine composition with white-skinned face function of releiving, it is characterised in that the bulk drug matched by following weight parts
It is made：
Oldenlandia diffusa 10~30, the root bark of Chinese wolf-berry 10~30, cactus 10~20, kuh-seng 10~20, ginkgo leaf 10~20, Radix Glycyrrhizae 10
~20, duck wheat 10~20.
2. external medicine composition as claimed in claim 1, it is characterised in that what the composition was matched by following weight parts
Bulk drug is constituted：
Oldenlandia diffusa 20~30, the root bark of Chinese wolf-berry 20~30, cactus 15~20, kuh-seng 15~20, ginkgo leaf 10~15, Radix Glycyrrhizae 10
~15, duck wheat 10~15.
3. the preparation method of external medicine composition described in a kind of claim 1 or 2, it is characterised in that comprise the following steps：
（1）Bulk drug is weighed according to weight described in claim 1 or 2；
（2）Percent by volume is that 75%~80% ethanol is extracted twice, and bulk drug and ethanol mass volume ratio g/ml are extracted for the first time
For 1：10~1：20,60 DEG C~70 DEG C heating stirrings 1~2 hour, filter to obtain first time filtrate；Second of extraction bulk drug and second
Alcohol mass volume ratio g/ml is 1：10~1：20,60 DEG C~70 DEG C heating stirring 30min~60min, filter to obtain second of filtrate；
First time filtrate and second of filtrate mixing, are concentrated into 0.2~0.5g/ml, obtain concentrate；
（3）It is 1 according to mass ratio：1~1：2 to step（2）Addition propane diols or butanediol in obtained concentrate, suction filtration,
3000~5000 revs/min of centrifuging and taking supernatants, are produced.
4. preparation method as claimed in claim 3, it is characterised in that the step（2）Middle first time extracts bulk drug and second
Alcohol mass volume ratio g/ml is 1：20；60 DEG C of heating stirrings 1 hour.
5. preparation method as claimed in claim 3, it is characterised in that the step（2）In second extract bulk drug and second
Alcohol mass volume ratio g/ml is 1：10；60 DEG C of heating stirrings 30 minutes.
6. a kind of external application Chinese medicine extract for having white-skinned face function of releiving, it is characterised in that the external application Chinese medicine extract is by right
It is required that any one methods described extracts what is obtained in 3 to 5.
7. external application Chinese medicine extract described in claim 6 is being prepared with the use in the external-use skin care preparation for releiving white-skinned face function
On the way.
8. a kind of skin care formulation with white-skinned face function of releiving, it is characterised in that the skin care formulation is as outer described in claim 6
It is made of Chinese medical extract and skin care field customary adjuvant.
9. a kind of external skin-care composition with white-skinned face function of releiving, it is characterised in that the external skin-care composition includes A
Phase B phases, C phases and D phases, A, B, C, D phase are made up of the raw material of following weight percentage：
10. the preparation method of external skin-care composition described in a kind of claim 9, it is characterised in that comprise the following steps：
（1）Double-PEG-18 methyl ethers dimethylsilanes first are heated into 40 DEG C~50 DEG C with water to be completely dissolved, it is stand-by；
（2）A phases：Water is added in aqueous phase tank after weighing up, by acrylic acid（Ester）Class/C10-30 alkylol acrylamide acid esters cross-linked polymers are equal
Even to be sprinkled upon water surface, after after immersion completely, other raw materials of addition A phases stir 30~50 revs/min, stirred；
（3）By step（2）The A phases prepared are pumped into emulsion tank, and vacuum is mixed, 30~50 revs/min of stir speed (S.S.),
It is heated to 80~85 DEG C and is incubated 30~40 minutes, stirring cooling；
（4）When temperature drops to 45~50 DEG C, B phase NaOH are added, are stirred under vacuum uniform；
（5）When temperature is dropped to below 40 DEG C, C phases and D phase raw materials are added, is stirred under vacuum uniformly, produces.
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| CN106562913B (en) * | 2015-10-10 | 2020-07-28 | 孟宏 | External composition with whitening effect, cosmetic preparation and preparation method thereof |
| CN106166256B (en) * | 2016-08-06 | 2019-09-20 | 北京国济众芳中医药研究所 | External medicine composition, preparation with antiallergic itching-relieving efficacies and preparation method thereof |
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| CN102247305B (en) * | 2010-05-18 | 2012-11-14 | 北京工商大学 | Extract capable of inhibiting ultraviolet ray damages on skin, and its preparation method and use |
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