CN103777000A - 一种用于免疫复合物缓冲解离剂的cic抗原缓冲液解离中和剂 - Google Patents
一种用于免疫复合物缓冲解离剂的cic抗原缓冲液解离中和剂 Download PDFInfo
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- CN103777000A CN103777000A CN201410033370.5A CN201410033370A CN103777000A CN 103777000 A CN103777000 A CN 103777000A CN 201410033370 A CN201410033370 A CN 201410033370A CN 103777000 A CN103777000 A CN 103777000A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/564—Immunoassay; Biospecific binding assay; Materials therefor for pre-existing immune complex or autoimmune disease, i.e. systemic lupus erythematosus, rheumatoid arthritis, multiple sclerosis, rheumatoid factors or complement components C1-C9
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/50—Determining the risk of developing a disease
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis
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Abstract
Description
方法 | 阳性数 | 阳性率(%) | OD值(x±s,A) |
实施例3 | 13 | 59.1 | 0.635±0.389 |
表面活性剂解离法 | 8 | 36.4 | 0.476±0.302 |
方法 | 阳性数 | 阳性率(%) | 定量结果(x±s,IU/ml) |
实施例3 | 21 | 56.8 | 37.6±39.2 |
强酸解离法 | 11 | 29.7 | 12.5±13.9 |
胃蛋白酶消化法 | 7 | 18.9 | 5.8±5.3 |
方法 | 阳性数 | 阳性率(%) | OD值(x±s,A) |
实施例3 | 49 | 94.2 | 0.876±0.532 |
C1q免疫捕获法 | 28 | 53.8 | 0.421±0.417 |
抗Ig免疫捕获法 | 37 | 71.2 | 0.598±0.481 |
病种 | 标本类型 | 例数 | 检测项目 | 实施方法 | 阳性率(%) |
乙肝患者 | 尿液 | 13 | HBsAg | 实施例3 | 38.5 |
不孕不育 | 血清 | 11 | 睾酮 | 实施例3 | 27.3 |
Claims (4)
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CN201410033370.5A CN103777000A (zh) | 2014-01-23 | 2014-01-23 | 一种用于免疫复合物缓冲解离剂的cic抗原缓冲液解离中和剂 |
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CN201410033370.5A CN103777000A (zh) | 2014-01-23 | 2014-01-23 | 一种用于免疫复合物缓冲解离剂的cic抗原缓冲液解离中和剂 |
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CN103777000A true CN103777000A (zh) | 2014-05-07 |
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CN201410033370.5A Pending CN103777000A (zh) | 2014-01-23 | 2014-01-23 | 一种用于免疫复合物缓冲解离剂的cic抗原缓冲液解离中和剂 |
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5187065A (en) * | 1989-12-22 | 1993-02-16 | Schutzer Steven E | Method and materials for detecting lyme disease |
US20060051744A1 (en) * | 2004-06-30 | 2006-03-09 | Austin Kimberly M | Feline infectious peritonitis (FIP) and systemic multi-organ coronavirus biomarkers and screening methods |
CN101832999A (zh) * | 2010-04-29 | 2010-09-15 | 山东莱博生物科技有限公司 | 一种抗原抗体复合物解离液试剂盒及应用 |
CN103308674A (zh) * | 2012-12-20 | 2013-09-18 | 重庆联佰博超医疗器械有限公司 | 过氧化物还原酶iv的循环免疫复合物及其应用 |
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2014
- 2014-01-23 CN CN201410033370.5A patent/CN103777000A/zh active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5187065A (en) * | 1989-12-22 | 1993-02-16 | Schutzer Steven E | Method and materials for detecting lyme disease |
US20060051744A1 (en) * | 2004-06-30 | 2006-03-09 | Austin Kimberly M | Feline infectious peritonitis (FIP) and systemic multi-organ coronavirus biomarkers and screening methods |
CN101832999A (zh) * | 2010-04-29 | 2010-09-15 | 山东莱博生物科技有限公司 | 一种抗原抗体复合物解离液试剂盒及应用 |
CN103308674A (zh) * | 2012-12-20 | 2013-09-18 | 重庆联佰博超医疗器械有限公司 | 过氧化物还原酶iv的循环免疫复合物及其应用 |
Non-Patent Citations (4)
Title |
---|
GRZEGORZ PRZYBYLSKI AND RYSZARD GOLDA: "Research on the occurrence of Mycobacterium tuberculosis antigens in the circulating immune complexes, isolated from serum of patients with tuberculosis", 《MED SCI MONIT》, vol. 20, 3 January 2014 (2014-01-03) * |
MARTÍN E RABASSA,ET AL: "MUC1 expression and anti-MUC1 serum immune response in head and neck squamous cell carcinoma (HNSCC): a multivariate analysis", 《BMC CANCER》, vol. 6, 25 October 2006 (2006-10-25), XP021023039, DOI: 10.1186/1471-2407-6-253 * |
吴炜 等: "重型乙型肝炎血清HBV特异性循环免疫复合物的检测及其意义", 《浙江医学》, vol. 27, no. 2, 31 December 2005 (2005-12-31) * |
成军 等: "免疫复合物解离剂研制及实验条件的研究", 《2012年浙江省检验医学学术年会论文集》, 16 August 2012 (2012-08-16) * |
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Inventor after: Cheng Jun Inventor after: Dai Yuzhu Inventor after: Jiang Xiaoxiao Inventor after: Zeng Xianming Inventor after: Wang Guozheng Inventor after: Sun Changgui Inventor before: Cheng Jun Inventor before: Ma Juming Inventor before: Chen Dawei Inventor before: Sun Changgui Inventor before: Dai Yuzhu Inventor before: Wang Guozheng Inventor before: Jiang Xiaoxiao Inventor before: Zhang Yiming |
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Application publication date: 20140507 |
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