CN103773002B - Anti-oxidant wrapping material of a kind of edibility sunflower protein and preparation method thereof - Google Patents
Anti-oxidant wrapping material of a kind of edibility sunflower protein and preparation method thereof Download PDFInfo
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- CN103773002B CN103773002B CN201410001166.5A CN201410001166A CN103773002B CN 103773002 B CN103773002 B CN 103773002B CN 201410001166 A CN201410001166 A CN 201410001166A CN 103773002 B CN103773002 B CN 103773002B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
- Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics
Abstract
The invention discloses anti-oxidant wrapping material of a kind of edibility sunflower protein and preparation method thereof, these anti-oxidant wrapping material comprise the component of following parts by weight: sunflower protein 100 parts, Ox blood plasma proteolysate 20 ~ 30 parts.The anti-oxidant wrapping material of edibility sunflower protein of the present invention, adopt Ox blood plasma proteolysate and sunflower protein to carry out composite, gained edible packing material, has good physical strength, ultraviolet isolating performance and water vapor through performance; Oxidation-resistance is obviously strengthened compared with simple sunflower protein film, Food Oxidation effectively can be prevented corrupt, thus reach the effect of food fresh keeping and Shelf-life; Gained wrapping material have edibility, can be biodegradable, can not to environment; Its raw material is renewable resources, and production cost is low, can replace other wrapping material, has greatly saved Nonrenewable resources, is applicable to applying.
Description
Technical field
The invention belongs to edible packing material technical field, be specifically related to the anti-oxidant wrapping material of a kind of edibility sunflower protein, also relate to the preparation method of the anti-oxidant wrapping material of a kind of edibility sunflower protein simultaneously.
Background technology
China is generally only confined to get its grease to the intensive processing of Sunflower Receptacle, and the sunflower seed dregs produced in the course of processing, then basic as cheap animal-feed, fail to obtain fully and effectively utilize.Ox blood is the byproduct after ox is butchered, and measures large and has very high value of exploiting and utilizing.But ox blood is but well utilized for a long time, be substantially in the situation gone out of use, not only protein resource runs off, and also causes serious environmental pollution, according to environmental administration's statistics, and the pollution load BOD of every kilogram of poultry blood
5for 150g, remove 1 kilogram of BOD
5load need spend Renminbi 1014 yuan, and control whole nation poultry blood discharges the environmental pollution caused, and need consume Renminbi about 100,000,000 yuan; In addition, some illegal retailer illegally utilizes poultry blood also to bring a series of food-safety problem; Therefore, the exploitation of poultry blood resource, should obtain enough attention.
Along with people's improving constantly food quality and safety requirements, and the enhancing of people's environmental consciousness, the functional edible food packaging film made with natural biologic material, has become the study hotspot of food packaging applications.The film forming material of edible film mainly comprises protein, carbohydrate and lipid etc.; The raw material being widely studied the protein edible film of report at present has soybean protein, mucedin, zein and collagen protein etc., but has no to report with sunflower protein to be the research that edible film aspect prepared by raw material.
Functional package film can add some activeconstituentss to improve the antioxidant property of film, anti-microbial property or nutritive value.Oxygenizement causes one of food spoilage chief reason, adds antioxidant and more and more come into one's own to prepare the wrapping material with antioxidant property.In recent years, some artificial synthetized oxidation preventive agents start to be applied in food product pack mould material.But because the security of synthetized oxidation preventive agent life-time service is under suspicion, it can not meet the requirement that people use, and people are in the urgent need to the appearance of Nantural non-toxic antioxidant.
Summary of the invention
The object of this invention is to provide the anti-oxidant wrapping material of a kind of edibility sunflower protein, solving the problem that security that existing edible packing material causes because using artificial synthetized oxidation preventive agent can not meet service requirements.
Second object of the present invention is to provide the preparation method of the anti-oxidant wrapping material of a kind of edibility sunflower protein.
In order to realize above object, the technical solution adopted in the present invention is: the anti-oxidant wrapping material of a kind of edibility sunflower protein, comprise the component of following parts by weight: sunflower protein 100 parts, Ox blood plasma proteolysate 20 ~ 30 parts.
Described sunflower protein is the sunflower seeds protein concentrate extracted from sunflower seed dregs.The method of described extraction is alkali extraction-acid precipitation.
Described sunflower protein is prepared by following methods:
A) get sunflower seed dregs powder, according to the ratio of solid-liquid ratio 1:13 ~ 17, sunflower seed dregs powder is added in distilled water, stir, regulate pH to be 8.5 ~ 9.5, centrifugal supernatant liquor and residue;
B) residue is extracted once by step method a) again, merge the supernatant liquor of extracted twice, obtain mixed liquor A;
C) regulate the pH of mixed liquor A to sunflower seed dregs albumen iso-electric point 4.5, stirring is also centrifugal, abandons supernatant liquor, after gained precipitation distilled water wash, adds distilled water and dissolves, obtain albumin milk according to the ratio of solid-liquid ratio 1.5 ~ 2.2:1;
D) by step c) gained albumin milk is after twice homogenization, and adjustment pH is 8.5 ~ 9.5, carries out spraying dry, obtains described sunflower protein.
Step a) described in sunflower seed dregs powder by sunflower seed dregs through high speed Universalpulverizer pulverize after cross 60 ~ 80 eye mesh screens make.
Step a) and steps d) in regulate the method for pH to be add the sodium hydroxide solution that concentration is 3mol/L; Step c) in regulate the method for pH of mixed liquor A to be add the hydrochloric acid that concentration is 3mol/L.
Step a) described in centrifugal rotating speed be 3500r/min, the time is 15 ~ 25min; ; Step c) described in centrifugal rotating speed be 3500r/min, the time is 20min.
Steps d) in, the pressure of described twice homogenization is respectively 2 × 10
5pa, 5 × 10
5pa; Described spray-dired temperature in is 170 ~ 190 DEG C, and temperature out is 80 ~ 90 DEG C.
Described Ox blood plasma proteolysate is prepared by following methods:
I) get Ox blood plasma albumen, be dissolved in distilled water and make Ox blood plasma protein solution, after 90 DEG C of water-bath preheatings, the pH regulating Ox blood plasma protein solution is 8.0, and temperature is 55 DEG C;
Ii) in Ox blood plasma protein solution, add Sumizyme MP start hydrolysis, keep the pH value of solution constant in hydrolytic process, make enzyme-deactivating through 90 DEG C of water-baths after hydrolysis, obtain hydrolyzed solution;
Iii) regulating step ii) pH of gained hydrolyzed solution is after 7.0, vacuum lyophilization, obtains described Ox blood plasma proteolysate.
Step I i) in, the amount adding Sumizyme MP makes the mass concentration ratio of basic protein enzyme-to-substrate be 1 ~ 2:100; Hydrolysis time is 120 ~ 150min.
A preparation method for the above-mentioned anti-oxidant wrapping material of edibility sunflower protein, comprises the following steps:
1) get sunflower protein and to be dissolved in distilled water obtaining sunflower protein solution, then add glycerine, stirred at ambient temperature makes it mix, and obtains mixed liquid B;
2) pH regulating mixed liquid B is 10 ~ 12, adds Ox blood plasma proteolysate, mixes, obtain film-casting liquid;
3) by step 2) gained film-casting liquid sprawls masking, and place in moisture eliminator again after drying, take off film, to obtain final product.
Step 1) in, the concentration of described sunflower protein solution is 5% (w/v); In described mixed liquid B, the concentration of glycerine is 1% ~ 2% (w/v).
Step 3) in, the temperature of described drying is 50 ~ 70 DEG C, and the time is 4 ~ 6h; The relative humidity of described moisture eliminator is 58%, and temperature is 20 ~ 30 DEG C; The time of placing in moisture eliminator is 48h.
By product when sunflower seed dregs is sunflower seeds liquefaction, wherein contains the high-quality protein of 29% ~ 43%.Ox blood be about live body heavy 8%, in the blood plasma obtained after separation, protein content is 6% ~ 8%, is valuable protein resource.Plasma proteins has good gel property, emulsifying property and foaminess.Research shows, the proteolytic enzyme protein hydrolysate under appropriate conditions of incorporating selectively, its hydrolysate has stronger anti-oxidant activity.
The anti-oxidant wrapping material of edibility sunflower protein of the present invention, adopt Ox blood plasma proteolysate and sunflower protein to carry out composite, gained edible packing material, has good physical strength, ultraviolet isolating performance and water vapor through performance; Ox blood plasma proteolysate is as a kind of natural antioxidants, edible packing material after interpolation is compared with simple sunflower protein film, oxidation-resistance is obviously strengthened, and Food Oxidation effectively can be prevented corrupt, thus reach the effect of food fresh keeping and Shelf-life; Gained wrapping material have edibility, can be biodegradable, can not to environment; Ox blood plasma proteolysate and sunflower protein, as natural biologic material, are renewable resourcess, wide material sources, and production cost is low, can replace other wrapping material, have greatly saved Nonrenewable resources, are applicable to applying.
The anti-oxidant wrapping material of edibility sunflower protein of the present invention, using the sunflower protein that extracts from the sunflower seed dregs base material as edible film, sunflower seed dregs is made the best use of everything, significantly improve the added value of sunflower seed dregs, again Ox blood plasma proteolysate is added in edible film as natural antioxidants, turn waste into wealth, a new outlet is found in the comprehensive utilization for ox blood.
The preparation method of the anti-oxidant wrapping material of edibility sunflower protein of the present invention, masking is sprawled after sunflower protein and Ox blood plasma proteolysate are made film-casting liquid, gained edibility oxidation-resistant film has good physical strength, ultraviolet isolating performance and water vapor through performance, Food Oxidation effectively can be prevented corrupt, thus reach the effect of food fresh keeping and Shelf-life; Preparation method of the present invention, raw material sources are extensive, technique is simple, easy to operate, and production cost is low, are applicable to large-scale industrial production.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
In the specific embodiment of the present invention, be raw materials usedly commercial goods, sunflower seed dregs is purchased from Shandong Hao Zheng Farming Ltd.; Ox blood plasma albumen (powdery) is purchased from Tianjin En Bi protein company limited, and protein content is 70%; (vigor is 10 to Sumizyme MP
6u/g) good and bio tech ltd, Shanghai is purchased from.
Embodiment 1
The anti-oxidant wrapping material of edibility sunflower protein of the present embodiment, comprise the component of following parts by weight: sunflower protein 100 parts, Ox blood plasma proteolysate 20 parts.
Described sunflower protein is prepared by following methods:
A) sunflower seed dregs powder (described sunflower seed dregs powder is crossed 60 eye mesh screens by sunflower seed dregs and made after high speed Universalpulverizer is pulverized) is got, according to the ratio of solid-liquid ratio 1:15 (w/v), sunflower seed dregs powder is added in distilled water, stir 1h, employing concentration is that the NaOH solution of 3mol/L regulates pH to be 9, centrifugal 20min under 3500r/min speed conditions, obtains supernatant liquor and residue;
B) residue is extracted once by step method a) again, merge the supernatant liquor of extracted twice, obtain mixed liquor A;
C) concentration is adopted to be that the pH of the hydrochloric acid adjustment mixed liquor A of 3mol/L is to sunflower seed dregs albumen iso-electric point 4.5, stir 30min, there is Precipitation, centrifugal 20min under 3500r/min speed conditions, abandon supernatant liquor, gained precipitation pH is after the distilled water wash twice of 4.0, adds distilled water and dissolves, obtain albumin milk according to the ratio of solid-liquid ratio 2:1 (w/v);
D) by step c) gained albumin milk is after twice homogenization, and the pressure of twice homogenization is respectively 2 × 10
5pa and 5 × 10
5pa is that the pH of the NaOH solution Function protein breast of 3mol/L is 9 by concentration, carries out spraying dry (temperature in is 170 DEG C, and temperature out is 80 DEG C), obtains described sunflower protein.
Described Ox blood plasma proteolysate is prepared by following methods:
I) get Ox blood plasma albumen, be dissolved in distilled water and make the Ox blood plasma protein solution that concentration is 5% (w/w), after 90 DEG C of water-bath preheating 10min, the pH regulating Ox blood plasma protein solution is 8.0, and temperature is 55 DEG C;
Ii) in Ox blood plasma protein solution, add Sumizyme MP start hydrolysis, the amount adding Sumizyme MP makes the mass concentration ratio of basic protein enzyme-to-substrate be 1:100; Constantly add the NaOH solution that concentration is 1mol/L in hydrolytic process, keep the pH value of hydrating solution constant, make enzyme-deactivating through 90 DEG C of water-bath 5min after hydrolysis 120min, obtain hydrolyzed solution;
Iii) by concentration being the hydrochloric acid regulating step ii of 1mol/L) pH of gained hydrolyzed solution is after 7.0, vacuum lyophilization, obtains pulverulent solids, is described Ox blood plasma proteolysate.
Gained Ox blood plasma proteolysate is sealed, preserves at 4 DEG C, for subsequent use.
The preparation method of the anti-oxidant wrapping material of edibility sunflower protein of the present embodiment, comprises the following steps:
1) getting sunflower protein to be dissolved in distilled water concentration is the sunflower protein solution of 5% (w/v), then adds glycerine, and stirred at ambient temperature 15min makes it mix, and obtains mixed liquid B; In described mixed liquid B, the concentration of glycerine is 1% (w/v);
2) regulate the pH of mixed liquid B to be 10 by the NaOH solution that concentration is 2mol/L, after stirring 15min, add Ox blood plasma proteolysate, mix, obtain film-casting liquid;
3) 10ml step 2 is got) gained film-casting liquid, spread into polystyrene dishes (64cm
2) upper masking, after 60 DEG C of dry 5h, then be placed in the moisture eliminator that relative humidity (RH) is 58%, temperature is 20 DEG C and place 48h, take off film, obtain edibility sunflower protein oxidation-resistant film.
Embodiment 2
The anti-oxidant wrapping material of edibility sunflower protein of the present embodiment, comprise the component of following parts by weight: sunflower protein 100 parts, Ox blood plasma proteolysate 25 parts.
Described sunflower protein is prepared by following methods:
A) sunflower seed dregs powder (described sunflower seed dregs powder is crossed 60 eye mesh screens by sunflower seed dregs and made after high speed Universalpulverizer is pulverized) is got, according to the ratio of solid-liquid ratio 1:14 (w/v), sunflower seed dregs powder is added in distilled water, stir 0.9h, employing concentration is that the NaOH solution of 3mol/L regulates pH to be 8.5, centrifugal 25min under 3500r/min speed conditions, obtains supernatant liquor and residue;
B) residue is extracted once by step method a) again, merge the supernatant liquor of extracted twice, obtain mixed liquor A;
C) concentration is adopted to be that the pH of the hydrochloric acid adjustment mixed liquor A of 3mol/L is to sunflower seed dregs albumen iso-electric point 4.5, stir 35min, there is Precipitation, centrifugal 20min under 3500r/min speed conditions, abandon supernatant liquor, gained precipitation pH is after the distilled water wash twice of 4.0, adds distilled water and dissolves, obtain albumin milk according to the ratio of solid-liquid ratio 2.2:1 (w/v);
D) by step c) gained albumin milk is after twice homogenization, and the pressure of twice homogenization is respectively 2 × 10
5pa and 5 × 10
5pa is that the pH of the NaOH solution Function protein breast of 3mol/L is 8.5 by concentration, carries out spraying dry (temperature in is 180 DEG C, and temperature out is 85 DEG C), obtains described sunflower protein.
Described Ox blood plasma proteolysate is prepared by following methods:
I) get Ox blood plasma albumen, be dissolved in distilled water and make the Ox blood plasma protein solution that concentration is 5% (w/w), after 90 DEG C of water-bath preheating 10min, the pH regulating Ox blood plasma protein solution is 8.0, and temperature is 55 DEG C;
Ii) in Ox blood plasma protein solution, add Sumizyme MP start hydrolysis, the amount adding Sumizyme MP makes the mass concentration ratio of basic protein enzyme-to-substrate be 1.5:100; Constantly add the NaOH solution that concentration is 1mol/L in hydrolytic process, keep the pH value of hydrating solution constant, make enzyme-deactivating through 90 DEG C of water-bath 5min after hydrolysis 135min, obtain hydrolyzed solution;
Iii) by concentration being the hydrochloric acid regulating step ii of 1mol/L) pH of gained hydrolyzed solution is after 7.0, vacuum lyophilization, obtains pulverulent solids, is described Ox blood plasma proteolysate.
Gained Ox blood plasma proteolysate is sealed, preserves at 4 DEG C, for subsequent use.
The preparation method of the anti-oxidant wrapping material of edibility sunflower protein of the present embodiment, comprises the following steps:
1) getting sunflower protein to be dissolved in distilled water concentration is the sunflower protein solution of 5% (w/v), then adds glycerine, and stirred at ambient temperature 15min makes it mix, and obtains mixed liquid B; In described mixed liquid B, the concentration of glycerine is 1.5% (w/v);
2) regulate the pH of mixed liquid B to be 11 by the NaOH solution that concentration is 2mol/L, after stirring 15min, add Ox blood plasma proteolysate, mix, obtain film-casting liquid;
3) 10ml step 2 is got) gained film-casting liquid, spread into polystyrene dishes (64cm
2) upper masking, after 60 DEG C of dry 5h, then be placed in the moisture eliminator that relative humidity (RH) is 58%, temperature is 20 DEG C and place 48h, take off film, obtain edibility sunflower protein oxidation-resistant film.
Embodiment 3
The anti-oxidant wrapping material of edibility sunflower protein of the present embodiment, comprise the component of following parts by weight: sunflower protein 100 parts, Ox blood plasma proteolysate 30 parts.
Described sunflower protein is prepared by following methods:
A) sunflower seed dregs powder (described sunflower seed dregs powder is crossed 80 eye mesh screens by sunflower seed dregs and made after high speed Universalpulverizer is pulverized) is got, according to the ratio of solid-liquid ratio 1:16 (w/v), sunflower seed dregs powder is added in distilled water, stir 1.1h, employing concentration is that the NaOH solution of 3mol/L regulates pH to be 9.5, centrifugal 15min under 3500r/min speed conditions, obtains supernatant liquor and residue;
B) residue is extracted once by step method a) again, merge the supernatant liquor of extracted twice, obtain mixed liquor A;
C) concentration is adopted to be that the pH of the hydrochloric acid adjustment mixed liquor A of 3mol/L is to sunflower seed dregs albumen iso-electric point 4.5, stir 25min, there is Precipitation, centrifugal 20min under 3500r/min speed conditions, abandon supernatant liquor, gained precipitation pH is after the distilled water wash twice of 4.0, adds distilled water and dissolves, obtain albumin milk according to the ratio of solid-liquid ratio 1.5:1 (w/v);
D) by step c) gained albumin milk is after twice homogenization, and the pressure of twice homogenization is respectively 2 × 10
5pa and 5 × 10
5pa is that the pH of the NaOH solution Function protein breast of 3mol/L is 9.5 by concentration, carries out spraying dry (temperature in is 190 DEG C, and temperature out is 90 DEG C), obtains described sunflower protein.
Described Ox blood plasma proteolysate is prepared by following methods:
I) get Ox blood plasma albumen, be dissolved in distilled water and make the Ox blood plasma protein solution that concentration is 5% (w/w), after 90 DEG C of water-bath preheating 10min, the pH regulating Ox blood plasma protein solution is 8.0, and temperature is 55 DEG C;
Ii) in Ox blood plasma protein solution, add Sumizyme MP start hydrolysis, the amount adding Sumizyme MP makes the mass concentration ratio of basic protein enzyme-to-substrate be 2:100; Constantly add the NaOH solution that concentration is 1mol/L in hydrolytic process, keep the pH value of hydrating solution constant, make enzyme-deactivating through 90 DEG C of water-bath 5min after hydrolysis 150min, obtain hydrolyzed solution;
Iii) by concentration being the hydrochloric acid regulating step ii of 1mol/L) pH of gained hydrolyzed solution is after 7.0, vacuum lyophilization, obtains pulverulent solids, is described Ox blood plasma proteolysate.
Gained Ox blood plasma proteolysate is sealed, preserves at 4 DEG C, for subsequent use.
The preparation method of the anti-oxidant wrapping material of edibility sunflower protein of the present embodiment, comprises the following steps:
1) getting sunflower protein to be dissolved in distilled water concentration is the sunflower protein solution of 5% (w/v), then adds glycerine, and stirred at ambient temperature 15min makes it mix, and obtains mixed liquid B; In described mixed liquid B, the concentration of glycerine is 2% (w/v);
2) regulate the pH of mixed liquid B to be 12 by the NaOH solution that concentration is 2mol/L, after stirring 15min, add Ox blood plasma proteolysate, mix, obtain film-casting liquid;
3) 10ml step 2 is got) gained film-casting liquid, spread into polystyrene dishes (64cm
2) upper masking, after 60 DEG C of dry 5h, then be placed in the moisture eliminator that relative humidity (RH) is 58%, temperature is 20 DEG C and place 48h, take off film, obtain edibility sunflower protein oxidation-resistant film.
Experimental example 1
The traditional performance of this experimental example to embodiment 1 ~ 3 gained edibility oxidation-resistant film measures.
Traditional performance measuring method is as follows:
(1) measuring method of film thickness
Select intact, uniform film, get 5 points at random, measure its thickness with screw micrometer, average.
(2) film pierces through the mensuration of performance (PS)
Adopt Texture instrument to measure, be placed between the homemade poly (methyl methacrylate) plate of two panels by complete uniform membrane sample to be measured, wherein there is the circular hole of 3.2cm at poly (methyl methacrylate) plate center, and surrounding is fixed by screw.Select the probe of diameter 2mm, it is 1mm/s that probe moves down speed.Pierce through intensity (PS, N/mm) calculation formula such as formula shown in (1).
In formula, F
pfor maximum force of piercing (N); L is the thickness (mm) of membrane sample.
(3) mensuration of film tensile property (TS)
Be cut into rectangular with sharp blade by film to be measured, and double faced adhesive tape is sticked respectively in case from fracture of root in drawing process in two ends, final effectively tensile elongation is 40 × 6mm.Use the stretching device of Texture instrument, draw speed is 1mm/s.Tensile strength (TS, Mpa) can calculate from pressure force-strain curve and obtain, and sees formula (2).
In formula, Ft is maximum pull (N); L is the thickness (mm) of membrane sample; W is the width (i.e. 6mm) of membrane sample.
4) mensuration of film water vapor transmission (WVP)
Adopt and intend cup method, by complete uniform film phonograph seal on the self-control synthetic glass moisture vapor transmission cup surface containing 5g Calcium Chloride Powder Anhydrous (0%RH), film exposed area is 19.6cm
2.Moisture vapor transmission cup device is put into climatic chamber (80%RH, 25 DEG C), measured the gain in weight of moisture vapor transmission cup every 24 hours, see formula (3).
In formula, W is the weightening finish (g) of moisture vapor transmission cup; X is the thickness (mm) of film; A is film exposed area (i.e. 19.6cm
2); T is interval time of measurement (i.e. 24h); △ P is the water vapour pressure reduction (Δ P=2.5347KPa) of film both sides.
(5) mensuration of protein film moisture content (MC)
Protein film is cut into size to fit, places it in aluminium box and claim its weight; Be placed in 105 DEG C of baking ovens and dry 8h, claim its weight after taking out, before and after drying according to film, changes in weight calculates protein film equilibrium moisture content, sees formula (4).
In formula, Δ m is changes in weight before and after film is dried, m
0for film dries front weight.
(6) mensuration of protein film solvability (S)
Taking quality is m
1the dry film of (≈ 0.1g) puts into the culture dish filling 40ml water, is pulled out by film, blots the moisture on film surface with filter paper, then be dried to constant weight under the condition of 105 DEG C, claim quality is m at 25 DEG C after dissolving 24h
2, calculate protein film solvability (S) according to changes in weight before and after film, see formula (5).
The performance measurement result of embodiment 1 ~ 3 gained edibility oxidation-resistant film is as shown in table 1.
The performance measurement result of table 1 embodiment 1 ~ 3 gained edibility oxidation-resistant film
Experimental example 2
The antioxygen property of this experimental example to embodiment 1 ~ 3 gained edibility oxidation-resistant film measures.
Measuring method is as follows:
(1) preparation of sample solution
Taking quality is m
1the dry film of (≈ 0.1g) puts into the Erlenmeyer flask filling 40ml water, and room temperature puts into shaking table, and rotating speed is 1000rpm jolting 24h, dissolves, then filter with filter paper, get supernatant liquor and measure with short its.
(2) FRAP (ferricreducing-antioxidantpower, Fe3+ reduction resistance of oxidation) method measures resistance of oxidation
In reaction tubes, add 0.1ml sample, more accurately add 3mlFRAP working fluid, 300 μ l distilled waters, mixing, accurate response 8min, read absorbance in 593nm place, distilled water returns to zero.With 100 ~ 1000 μm of ol/LFeSO
47H
2o production standard curve.The anti-oxidant activity (FRAPvalue) of sample is to reach the FeSO needed for same absorbance
4mmole number represents, every part of sample replication 3 times.FRAP working fluid matching while using, by the 100ml30mmol/L acetate buffer of pH3.6,10ml10mmol/LTPTZ solution and 10ml20mmol/LFeCl
36H
2o solution mixes composition.
(3) mensuration of anti peroxidation of lipid ability
Liposome is obtained by soybean lecithin, soybean lecithin is dissolved (200mg/L) after 0.12mol/LKCl, 5mmol/L histidine buffer solution (pH value 6.8) homogeneous, carries out 45min ultrasonication at 4 DEG C, dissolve with short its.
By 5ml liposome solutions and 1ml sample mix; Contrast is for replacing 1ml sample to mix with 5ml lipid with 1ml distilled water.Add 0.1ml50mmol/LFeCl
3cause lipid oxidation with 0.1ml10mmol/L sodium ascorbate, be then placed on by sample in 37 DEG C of water-baths and be incubated 1h, its degree of oxidation is measured by thiobarbituricacidα-(TBA) method.After sample solution and 0.02mol/LTBA react, mix with chloroform 1:1 (v/v), vortex vibrates, the centrifugal 10min of 1800g.Get the light absorption value that supernatant is determined at 532nm place.Formula (6) is shown in the calculation formula of lipid oxidation inhibiting rate.
To lipid oxidation inhibiting rate (%)=[1-(light absorption value/contrast solution light absorption value of sample)] × 100% (6)
The antioxygen property measurement result of embodiment 1 ~ 3 gained edibility oxidation-resistant film is as shown in table 2.
Wherein, comparative example is the sunflower protein film not adding Ox blood plasma proteolysate; Its preparation method with embodiment 1, but does not add Ox blood plasma proteolysate in film-casting liquid.
The antioxygen property measurement result of table 2 embodiment 1 ~ 3 gained edibility oxidation-resistant film
Claims (10)
1. the anti-oxidant wrapping material of edibility sunflower protein, is characterized in that: the component comprising following parts by weight: sunflower protein 100 parts, Ox blood plasma proteolysate 20 ~ 30 parts.
2. the anti-oxidant wrapping material of edibility sunflower protein according to claim 1, is characterized in that: described sunflower protein is prepared by following methods:
A) get sunflower seed dregs powder, according to the ratio of solid-liquid ratio 1:13 ~ 17, sunflower seed dregs powder is added in distilled water, stir, regulate pH to be 8.5 ~ 9.5, centrifugal supernatant liquor and residue;
B) residue is extracted once by step method a) again, merge the supernatant liquor of extracted twice, obtain mixed liquor A;
C) regulate the pH of mixed liquor A to sunflower seed dregs albumen iso-electric point 4.5, stirring is also centrifugal, abandons supernatant liquor, after gained precipitation distilled water wash, adds distilled water and dissolves, obtain albumin milk according to the ratio of solid-liquid ratio 1.5 ~ 2.2:1;
D) by step c) gained albumin milk is after twice homogenization, and adjustment pH is 8.5 ~ 9.5, carries out spraying dry, obtains described sunflower protein.
3. the anti-oxidant wrapping material of edibility sunflower protein according to claim 2, is characterized in that: step a) and steps d) in regulate the method for pH to be add the sodium hydroxide solution that concentration is 3mol/L; Step c) in regulate the method for pH of mixed liquor A to be add the hydrochloric acid that concentration is 3mol/L.
4. the anti-oxidant wrapping material of edibility sunflower protein according to claim 2, is characterized in that: step a) described in centrifugal rotating speed be 3500r/min, the time is 15 ~ 25min; ; Step c) described in centrifugal rotating speed be 3500r/min, the time is 20min.
5. the anti-oxidant wrapping material of edibility sunflower protein according to claim 2, is characterized in that: steps d) in, the pressure of described twice homogenization is respectively 2 × 10
5pa, 5 × 10
5pa; Described spray-dired temperature in is 170 ~ 190 DEG C, and temperature out is 80 ~ 90 DEG C.
6. the anti-oxidant wrapping material of edibility sunflower protein according to claim 1, is characterized in that: described Ox blood plasma proteolysate is prepared by following methods:
I) get Ox blood plasma albumen, be dissolved in distilled water and make Ox blood plasma protein solution, after 90 DEG C of water-bath preheatings, the pH regulating Ox blood plasma protein solution is 8.0, and temperature is 55 DEG C;
Ii) in Ox blood plasma protein solution, add Sumizyme MP start hydrolysis, keep the pH value of solution constant in hydrolytic process, make enzyme-deactivating through 90 DEG C of water-baths after hydrolysis, obtain hydrolyzed solution;
Iii) regulating step ii) pH of gained hydrolyzed solution is after 7.0, vacuum lyophilization, obtains described Ox blood plasma proteolysate.
7. the anti-oxidant wrapping material of edibility sunflower protein according to claim 6, is characterized in that: step I i) in, the amount adding Sumizyme MP makes the mass concentration ratio of basic protein enzyme-to-substrate be 1 ~ 2:100; Hydrolysis time is 120 ~ 150min.
8. a preparation method for the anti-oxidant wrapping material of edibility sunflower protein as claimed in claim 1, is characterized in that: comprise the following steps:
1) get sunflower protein and to be dissolved in distilled water obtaining sunflower protein solution, then add glycerine, stirred at ambient temperature makes it mix, and obtains mixed liquid B;
2) pH regulating mixed liquid B is 10 ~ 12, adds Ox blood plasma proteolysate, mixes, obtain film-casting liquid;
3) by step 2) gained film-casting liquid sprawls masking, and place in moisture eliminator again after drying, take off film, to obtain final product.
9. the preparation method of the anti-oxidant wrapping material of edibility sunflower protein according to claim 8, is characterized in that: step 1) in, the concentration of described sunflower protein solution is 5% (w/v); In described mixed liquid B, the concentration of glycerine is 1% ~ 2% (w/v).
10. the preparation method of the anti-oxidant wrapping material of edibility sunflower protein according to claim 8, is characterized in that: step 3) in, the temperature of described drying is 50 ~ 70 DEG C, and the time is 4 ~ 6h; The relative humidity of described moisture eliminator is 58%, and temperature is 20 ~ 30 DEG C; The time of placing in moisture eliminator is 48h.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1095033A (en) * | 1993-05-11 | 1994-11-16 | 符玉华 | Edible film packing material |
| CN101580595A (en) * | 2009-06-18 | 2009-11-18 | 沈阳农业大学 | Method for preparing edible composite protein film |
| CN101851424A (en) * | 2009-03-31 | 2010-10-06 | 西南科技大学 | Thermoplastic plant protein/polylactic acid blend material and preparation method thereof |
| CN102191304A (en) * | 2011-03-23 | 2011-09-21 | 连云港福润食品有限公司 | Porcine plasma antioxidant peptide |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1095033A (en) * | 1993-05-11 | 1994-11-16 | 符玉华 | Edible film packing material |
| CN101851424A (en) * | 2009-03-31 | 2010-10-06 | 西南科技大学 | Thermoplastic plant protein/polylactic acid blend material and preparation method thereof |
| CN101580595A (en) * | 2009-06-18 | 2009-11-18 | 沈阳农业大学 | Method for preparing edible composite protein film |
| CN102191304A (en) * | 2011-03-23 | 2011-09-21 | 连云港福润食品有限公司 | Porcine plasma antioxidant peptide |
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