CN103333509A - Edible fish skin collagen antibacterial film containing thyme essential oil - Google Patents
Edible fish skin collagen antibacterial film containing thyme essential oil Download PDFInfo
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- CN103333509A CN103333509A CN2013102722366A CN201310272236A CN103333509A CN 103333509 A CN103333509 A CN 103333509A CN 2013102722366 A CN2013102722366 A CN 2013102722366A CN 201310272236 A CN201310272236 A CN 201310272236A CN 103333509 A CN103333509 A CN 103333509A
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Abstract
The invention discloses an edible fish skin collagen antibacterial film containing thyme essential oil and a preparation method thereof. The edible fish skin collagen antibacterial film comprises the following components by weight percent: 4 percent of fish skin collagen, 4 percent of thyme essential oil, 0.25 percent of soya bean lecithin, 1 percent of glycerinum and the balance of distilled water. The preparation method comprises the following steps of: firstly dissolving the fish skin collagen powder into the distilled water to prepare the fish skin collagen solution, then dissolving the thyme essential oil into the distilled water of the soya bean lecithin to prepare the thyme essential oil solution, mixing the fish skin collagen solution and the thyme essential oil solution, adding glycerinum to be used as a plasticizer, uniformly mixing, standing the mixture, degassing, and moving and paving the mixture onto an organic glass frame to be dried and formed into a film. The collagen antibacterial film has good antibacterial performance, can be degraded by organisms, is free from polluting the environment and can inhibit the growth of microorganism on the surface of food so as to keep the freshness of the food and prolong the shelf life.
Description
Technical field
The invention belongs to the applied technical field of antimicrobial packaging film, be specifically related to a kind of edibility collagen of fish skin antibacterial film that contains thyme oil and preparation method thereof.
Background technology
In recent years, people more and more pay close attention to environmental pollution and food-safety problem that Plastic Packaging Materials causes along with the enhancing of environmental consciousness, and packaging material for food is had higher requirement.Therefore, utilize natural biologic material to be subjected to showing great attention to of people for the edible packing membrane of feedstock production.Edible film (Edible film) refers to that with edible natural material (as polysaccharide, protein, lipid etc.) be raw material, by the dense film with porous network structure that the differing molecular interphase interaction forms, can be used for food product pack and aspect such as fresh-keeping.Can add some antiseptic-germicides in the edible film with the anti-microbial property of raising film, thereby more effectively prolong the quality guaranteed period of food.The edibility antibacterial film is to add antiseptic-germicide in edible film, reaches a kind of active film antibiotic, fresh-keeping effect.Antiseptic-germicide mainly is divided into 3 big class, i.e. inorganic antiseptic, synthetic antibacterial agents and natural antibacterial agents.Inorganic antiseptic has good heat resistance, antibacterial range and extensively, does not produce advantages such as resistance, synthetic antibacterial agents mainly is that sterilization speed is fast, to press down mould effective, but all there are food safety question in inorganic antiseptic and synthetic antibacterial agents, and natural antibacterial agent has excellent biological compatibility and environmental-protecting performance, toxicity is less, does not have the food safety potential problem; Therefore, the Application and Development of natural antibacterial agent receives increasing concern.
Because thyme oil has its natural characteristic such as antibiotic and anti-oxidant. become the synthetic additive in the food-processing industry in recent years gradually.Ideal substitute as preservation agent, antiseptic-germicide, antioxidant, stablizer etc.Based on its numerous good characteristics, in the ascendant with exploitation to the Thymus Study on plants in the world.The wild Thymus plant resources of China is abundant, further research and development utilize this platymiscium significant, have a extensive future.
Summary of the invention
The object of the present invention is to provide a kind of edibility collagen of fish skin antibacterial film that contains thyme oil and preparation method thereof, raw material sources of the present invention are extensive, and technology is simple; The thyme oil Nantural non-toxic, and have good bacteriostatic activity, the antibacterial film of preparation is functional and have the effect that suppresses the food surfaces microorganism growth, thus the effect that reaches food fresh keeping and prolong shelf-lives.
For achieving the above object, the present invention adopts following technical scheme:
A kind of feed composition that contains the edibility collagen of fish skin antibacterial film of thyme oil is by weight percentage: collagen of fish skin 4%, and thyme oil 4%, soybean lecithin 0.25%, glycerine 1%, surplus is distilled water.
Preparing the aforesaid method that contains the edibility collagen of fish skin antibacterial film of thyme oil may further comprise the steps:
(1) preparation of collagen of fish skin solution: add 15 ml distilled water in 1.2 g collagen of fish skin powder, collagen of fish skin solution is made in 45 ℃ of magnetic agitation dissolvings;
(2) preparation of thyme oil solution: add the 15 ml distilled water that contain 0.075 g soybean lecithin in the thyme oil of 1.2 ml, the homogeneous stirring and dissolving makes cinnamon essential oil solution;
(3) preparation of antibacterial film: the collagen of fish skin solution of step (1), the thyme oil solution of step (2) are mixed, add 0.3g glycerine again as softening agent, stir, leave standstill the degassing, get the antibacterial film film-casting liquid, 45 ℃ of insulations; Pipette 10 ml film-casting liquids at every turn and spread into film forming on 120 mm * 80 mm * 2 mm synthetic glass frames, film-forming temperature is 22 ℃, and film formation time is 48-24 h, makes edibility collagen of fish skin antibacterial film.
The preparation technology of the collagen of fish skin powder described in the step (1) is as follows: the waste fish-skin is scaled, and shreds cleanly, and the contain volume fraction heavy with 20 times of raw materials is 1% H
2O
20.01mo/LNaOH solution stir to soak 24 h at 20 ℃, be washed till neutrality with clear water; At room temperature stir immersion 4 h with 20 times of heavy 0.05mo/L acetic acid or citric acid solutions of raw material again, be washed till neutrality with clear water; Then, lixiviate 12 h in 45 ℃ of heavy hot water of 10 times of raw materials; Adopt centrifugation to remove insoluble substance, freeze-drying namely gets the collagen of fish skin powder.The processing fish-skin by product that described waste fish-skin is silver carp, grass carp, black carp, bighead, shark or eel.
The thyme oil color that the present invention adopts is moderate yellowish brown and somewhat green, and have strong herbal medicine and pine tar fragrance, its main component is thymol (22.72%), isothymol (15.40%), isothymol (11.57%) and to a P-cymene (9.13%).Studies show that in a large number Thymus vulgaris and extract thereof have opposing bacterium, fungi, virus, parasite, spasmolytic and anti-oxidant activity.Again owing to become synthetic additive in the food-processing industry in recent years, therefore it is added to freshness-retained food in the food and prolong the shelf-lives of food.The edible film that contains plants essential oil not only can be killed the bacterium with the film contact site, can also rely on volatile matter to suppress the growth of bacterium around the film, increases its antibacterial sphere of action greatly.
Beneficial effect of the present invention is:
(1) the collagen protein antibacterial film of the present invention's preparation has good antimicrobial property, can be by biological degradation, can not pollute environment, can suppress the food surfaces microbial growth, thereby the effect that reaches food fresh keeping and prolong shelf-lives, when being added with the composite film packaging food of thyme oil, freshness date was prolonged about a week.
(2) raw material sources among the preparation method of the present invention are extensive, and technology is simple; The thyme oil Nantural non-toxic, and have good bacteriostatic activity.
Embodiment
Embodiment 1
Take by weighing 1.2 g collagen proteins and make the collagen protein aqueous solution with 15 ml distilled water stirring and dissolving in 45 ℃ of stirring water-baths; Thyme oil solution is made in thyme oil homogeneous dissolving in containing 15 ml distilled water of 0.075 g soybean lecithin of 0.6ml, then with the two mixing, added 0.3 g glycerine as softening agent, stir the ultrasonic 30 min degassing, 45 ℃ of insulations.The film-casting liquid that at every turn pipettes 10 ml spreads into film forming on 120 mm * 80 mm * 2 mm synthetic glass frames, and film-forming temperature is 22 ℃, and film formation time is 24 h, can peel off from the synthetic glass frame after film is done by the time and obtain the collagen protein antibacterial film.Adopt the filter paper method to measure the film anti-microbial activity, film is intercepted the circle of 12 mm, investigate antibacterial film respectively to the fungistatic effect of intestinal bacteria and streptococcus aureus.
Embodiment 2
Take by weighing 1.2 g collagen proteins and make the collagen protein aqueous solution with 15 ml distilled water stirring and dissolving in 45 ℃ of stirring water-baths; Thyme oil solution is made in thyme oil homogeneous dissolving in containing 15 ml distilled water of 0.075 g soybean lecithin of 1.2 ml, then the two solution is mixed, add 0.3 g glycerine as softening agent, stir, the ultrasonic 30 min degassing, 45 ℃ of insulations.The film-casting liquid that at every turn pipettes 10 ml spreads into film forming on 120 mm * 80 mm * 2 mm synthetic glass frames, and film-forming temperature is 4 ℃, and film formation time is 24 h, can peel off from the synthetic glass frame after film is done by the time and obtain the collagen protein antibacterial film.Adopt the filter paper method to measure the film anti-microbial activity, film is intercepted the circle of 12 mm, investigate antibacterial film respectively to the fungistatic effect of intestinal bacteria and streptococcus aureus.
The inhibition zone of the antibacterial film for preparing under table 1 different condition
As seen from the above table: it is zero that the collagem membrane that does not add thyme oil suppresses to enclose; after adding thyme oil; as seen tangible inhibition zone; and thyme oil will be better than intestinal bacteria slightly to the fungistatic effect of streptococcus aureus; this mainly is because streptococcus aureus belongs to gram-positive microorganism; and intestinal bacteria belong to Gram-negative bacteria, and the provide protection of gram-negative bacteria cell wall lipopolysaccharides and outer membrane albumen makes that its fungistatic effect is more weak.
The mensuration of film thickness:
Adopt the thickness of 6 different positionss of screw micrometer random measurement film sample, average as the thickness of this film.
The mensuration of film dynamic performance:
Select the smooth film that does not have the cavity, film is cut into the rectangular of 2cm * 6 cm, measure tensile strength and elongation at break with food matter structure instrument.The tension rate setting of matter structure instrument is 1mm/s, and all films are placed on 22 ℃, and relative humidity is 24h in 60% the growth cabinet, and is to be measured.The film of every kind of ratio is done 3 parallel tests, and the result gets its mean value.
Tensile strength (TS) value is represented divided by sectional area with maximum pull:
TS=Fm/(L×W)。In the formula, TS is tensile strength (MPa); The maximum tension (N) that Fm bears during for sample fracture; L is the thickness (mm) of film; W is the width (mm) of film.
Elongation at break (EAB) is calculated as follows:
EAB=(Δ L/L
0) * 100%=[(L
1-L
0)/L
0In] * 100% formula, L
0Original length (mm) for film; The length (mm) that Δ L is stretched when being the film fracture; L
1The maximum length that reaches when rupturing for film (mm).
Table 2 different concns thyme oil is to the influence of film properties
As seen from the above table, along with essential oil concentration increases, its thickness increases.This is because essential oil is lyophobic dust, and after adding essential oil, the non-compatibility of it and collagem membrane causes the microtexture of film to become loose; When therefore essential oil concentration increased, the thickness of composite membrane also increased.More former film is compared, and variation has also taken place physical strength (tensile strength reduces, and elongation at break reduces), and this may be that the inconsistent loss that causes its physical strength becomes because after adding essential oil, the microcosmos network structure of film is damaged.
The above only is the preferred embodiment of invention, and all equalizations of doing according to the present patent application claim change and modify, and all should belong to covering scope of the present invention.
Claims (4)
1. edibility collagen of fish skin antibacterial film that contains thyme oil, it is characterized in that: the feed composition of described antibacterial film is by weight percentage: collagen of fish skin 4%, thyme oil 4%, soybean lecithin 0.25%, glycerine 1%, surplus are distilled water.
2. one kind prepares the method that contains the edibility collagen of fish skin antibacterial film of thyme oil as claimed in claim 1, it is characterized in that: may further comprise the steps:
(1) preparation of collagen of fish skin solution: collagen of fish skin is complete with dissolved in distilled water, makes collagen of fish skin solution;
(2) preparation of thyme oil solution: thyme oil homogeneous stirring and dissolving in containing the distilled water of soybean lecithin is complete, makes thyme oil solution;
(3) preparation of antibacterial film: the collagen of fish skin solution of step (1) and the thyme oil solution of step (2) are mixed, add glycerine again as softening agent, last 22 ℃ of film forming 48-24 h make the edibility collagen of fish skin antibacterial film that contains thyme oil.
3. the preparation method who contains the edibility collagen of fish skin antibacterial film of thyme oil according to claim 2, it is characterized in that: the preparation technology of the collagen of fish skin described in the step (1) is as follows: the waste fish-skin is scaled, shredding cleanly, is 1% H with containing volume fraction
2O
20.01 mo/L NaOH solution stir down at 20 ℃ and soak 24 h, be washed till neutrality with clear water; At room temperature stir with 0.05 mo/L acetic acid or citric acid solution again and soak 4 h, be washed till neutrality with clear water; Behind lixiviate 12 h under 45 ℃ of hot water conditions, adopt centrifugation to remove insoluble substance then, freeze-drying namely gets the collagen of fish skin powder.
4. the preparation method who contains the edibility collagen of fish skin antibacterial film of thyme oil according to claim 3, it is characterized in that: described waste fish-skin is a kind of processing byproduct in silver carp, grass carp, black carp, bighead, shark, the eel.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105111511A (en) * | 2015-07-31 | 2015-12-02 | 河南科技大学 | Antibacterial nano-composite edible film and preparation method therefor |
| CN105199401A (en) * | 2015-09-02 | 2015-12-30 | 天津现代职业技术学院 | Edible antibacterial calcium supplementing packaging film and preparation method thereof |
| CN106317909A (en) * | 2016-08-25 | 2017-01-11 | 董晓 | Preparing method of antibacterial medical grade thin film material |
| CN106433150A (en) * | 2016-09-27 | 2017-02-22 | 江南大学 | Red snapper fish scale protein film and preparation method thereof |
| CN110437625A (en) * | 2019-06-25 | 2019-11-12 | 浙江省农业科学院 | A kind of edible film-coating and preparation method thereof |
| CN110720580A (en) * | 2019-10-19 | 2020-01-24 | 浙江海洋大学 | Preparation method of thyme crude extract compound fish scale collagen food preservative film |
| CN110760080A (en) * | 2019-10-10 | 2020-02-07 | 浙江海洋大学 | Method for preparing preservative film by using fish scales |
| CN111588654A (en) * | 2020-06-08 | 2020-08-28 | 大连工业大学 | Edible antibacterial oleogel and preparation method thereof |
Citations (1)
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| CN103030979A (en) * | 2012-12-27 | 2013-04-10 | 福建省博特生物科技有限公司 | Collagen antibacterial membrane containing cinnamon essential oil and preparation method of collagen antibacterial membrane |
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Patent Citations (1)
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| CN103030979A (en) * | 2012-12-27 | 2013-04-10 | 福建省博特生物科技有限公司 | Collagen antibacterial membrane containing cinnamon essential oil and preparation method of collagen antibacterial membrane |
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| C. PIRES ET AL.: "Hake proteins edible films incorporated with essential oils: Physical， mechanical，antioxidant and antibacterial properties", 《FOOD HYDROCOLLOIDS》 * |
| 张赟彬 等: "可食膜的研究进展", 《中国食品添加剂》 * |
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105111511A (en) * | 2015-07-31 | 2015-12-02 | 河南科技大学 | Antibacterial nano-composite edible film and preparation method therefor |
| CN105111511B (en) * | 2015-07-31 | 2017-12-19 | 河南科技大学 | A kind of compound edible film of antimicrobial nano and preparation method thereof |
| CN105199401A (en) * | 2015-09-02 | 2015-12-30 | 天津现代职业技术学院 | Edible antibacterial calcium supplementing packaging film and preparation method thereof |
| CN105199401B (en) * | 2015-09-02 | 2017-12-26 | 天津现代职业技术学院 | A kind of edible antibacterial is replenished the calcium packaging film and preparation method thereof |
| CN106317909A (en) * | 2016-08-25 | 2017-01-11 | 董晓 | Preparing method of antibacterial medical grade thin film material |
| CN106433150A (en) * | 2016-09-27 | 2017-02-22 | 江南大学 | Red snapper fish scale protein film and preparation method thereof |
| CN110437625A (en) * | 2019-06-25 | 2019-11-12 | 浙江省农业科学院 | A kind of edible film-coating and preparation method thereof |
| CN110437625B (en) * | 2019-06-25 | 2021-08-31 | 浙江省农业科学院 | Edible coating film and preparation method thereof |
| CN110760080A (en) * | 2019-10-10 | 2020-02-07 | 浙江海洋大学 | Method for preparing preservative film by using fish scales |
| CN110760080B (en) * | 2019-10-10 | 2022-06-21 | 浙江海洋大学 | Method for preparing preservative film by using fish scales |
| CN110720580A (en) * | 2019-10-19 | 2020-01-24 | 浙江海洋大学 | Preparation method of thyme crude extract compound fish scale collagen food preservative film |
| CN111588654A (en) * | 2020-06-08 | 2020-08-28 | 大连工业大学 | Edible antibacterial oleogel and preparation method thereof |
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Application publication date: 20131002 |