CN103773002A - Edible sunflower seed protein antioxidant packaging material and preparation method thereof - Google Patents

Edible sunflower seed protein antioxidant packaging material and preparation method thereof Download PDF

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Publication number
CN103773002A
CN103773002A CN201410001166.5A CN201410001166A CN103773002A CN 103773002 A CN103773002 A CN 103773002A CN 201410001166 A CN201410001166 A CN 201410001166A CN 103773002 A CN103773002 A CN 103773002A
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sunflower
protein
edibility
blood plasma
oxidant
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CN103773002B (en
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张慧芸
康怀彬
李鑫玲
张敏
庄军辉
郭新宇
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Henan University of Science and Technology
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Henan University of Science and Technology
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/90—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in food processing or handling, e.g. food conservation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W90/00—Enabling technologies or technologies with a potential or indirect contribution to greenhouse gas [GHG] emissions mitigation
    • Y02W90/10—Bio-packaging, e.g. packing containers made from renewable resources or bio-plastics

Abstract

The invention discloses an edible sunflower seed protein antioxidant packaging material and a preparation method thereof. The antioxidant packaging material comprises the following components in parts by weight: 100 parts of sunflower seed protein, and 20-30 parts of bovine plasma protein hydrolysate. According to the edible sunflower seed protein antioxidant packaging material provided by the invention, the bovine plasma protein hydrolysate is compounded with the sunflower seed protein, so that the obtained edible packaging material has good mechanical strength, ultraviolet barrier properties and water vapor transmission resistance. Compared with a simple sunflower seed protein membrane, the resistance to oxidation is obviously enhanced, and oxidative rotting of the food can be effectively prevented, so that the effects of preserving the food and prolonging the shelf life are achieved; the obtained packaging material has edibility, can be biodegraded, and does not lead to pollution to the environment; the raw materials are renewable resources; the packaging material is low in production cost, and can replace other packaging materials; the non-renewable resources are greatly saved; the edible sunflower seed protein antioxidant packaging material is applicable to popularization and application.

Description

Anti-oxidant wrapping material of a kind of edibility sunflower protein and preparation method thereof
Technical field
The invention belongs to edible packing material technical field, be specifically related to the anti-oxidant wrapping material of a kind of edibility sunflower protein, also relate to the preparation method of the anti-oxidant wrapping material of a kind of edibility sunflower protein simultaneously.
Background technology
China is generally only confined to get its grease to the intensive processing of Sunflower Receptacle, and the sunflower seed dregs producing in the course of processing is basic as cheap animal-feed, fails to obtain fully and effectively utilizes.Ox blood is the byproduct after ox is butchered, and amount is large and have a very high value of exploiting and utilizing.But ox blood is but well utilized for a long time, the situation in going out of use substantially, not only protein resource runs off, and also causes serious environmental pollution, according to environmental administration's statistics, the pollution load BOD of every kilogram of poultry blood 5for 150g, remove 1 kilogram of BOD 5load needs 1014 yuan of cost Renminbi, and control whole nation poultry blood discharges the environmental pollution causing, and needs to consume approximately 100,000,000 yuan of Renminbi; In addition, some illegal retailer illegally utilizes poultry blood also to bring a series of food-safety problems; Therefore, the exploitation of poultry blood resource, should obtain enough attention.
Along with people are to the improving constantly of food quality and safety requirements, and the enhancing of people's environmental consciousness, the functional edible food packaging film made from natural biologic material, has become the study hotspot in food product pack field.The film forming material of edible film mainly comprises protein, carbohydrate and lipid etc.; The raw material that is widely studied at present the protein edible film of report has soybean protein, mucedin, zein and collagen protein etc., but has no report is prepared edible film aspect research take sunflower protein as raw material.
Functional package film can add some activeconstituentss to improve antioxidant property, anti-microbial property or the nutritive value of film.Oxygenizement is to cause one of food spoilage chief reason, and the wrapping material that interpolation antioxidant has antioxidant property with preparation more and more come into one's own.In recent years, some artificial synthetized oxidation preventive agents start to be applied in food product pack mould material.But because the security of synthetized oxidation preventive agent life-time service is under suspicion, it can not meet the requirement that people use, people are in the urgent need to the appearance of Nantural non-toxic antioxidant.
Summary of the invention
The object of this invention is to provide the anti-oxidant wrapping material of a kind of edibility sunflower protein, solve existing edible packing material and can not meet the problem of service requirements because of the security that uses artificial synthetized oxidation preventive agent and cause.
Second object of the present invention is to provide the preparation method of the anti-oxidant wrapping material of a kind of edibility sunflower protein.
In order to realize above object, the technical solution adopted in the present invention is: the anti-oxidant wrapping material of a kind of edibility sunflower protein, the component that comprises following parts by weight: 100 parts of sunflower proteins, 20～30 parts of Ox blood plasma proteolysates.
Described sunflower protein is the sunflower seeds protein concentrate extracting from sunflower seed dregs.The method of described extraction is alkali extraction and acid precipitation method.
Described sunflower protein is prepared by following methods:
A) get sunflower seed dregs powder, according to the ratio of solid-liquid ratio 1:13～17, sunflower seed dregs powder is added in distilled water, stir, regulating pH is 8.5～9.5, centrifugal supernatant liquor and the residue of obtaining;
B) residue is extracted once by the method for step a) again, merge the supernatant liquor of extracted twice, obtain mixed liquor A;
C) regulate the pH of mixed liquor A to sunflower seed dregs albumen iso-electric point 4.5, stirring is also centrifugal, abandons supernatant liquor, and gained precipitates with after distilled water wash, adds distilled water to dissolve according to the ratio of solid-liquid ratio 1.5～2.2:1, obtains albumin milk;
D) by step c) gained albumin milk after twice homogeneous, regulate pH be 8.5～9.5, spray be dried, obtain described sunflower protein.
The powder of sunflower seed dregs described in step a) is crossed 60～80 eye mesh screens and is made after high speed Universalpulverizer is pulverized by sunflower seed dregs.
The method that regulates pH in step a) and step d) is that to add concentration be the sodium hydroxide solution of 3mol/L; The method that regulates the pH of mixed liquor A in step c) is that to add concentration be the hydrochloric acid of 3mol/L.
Described in step a), centrifugal rotating speed is 3500r/min, and the time is 15～25min; ; Described in step c), centrifugal rotating speed is 3500r/min, and the time is 20min.
In step d), the pressure of described twice homogeneous is respectively 2 × 10 5pa, 5 × 10 5pa; Described spray-dired temperature in is 170～190 ℃, and temperature out is 80～90 ℃.
Described Ox blood plasma proteolysate is prepared by following methods:
I) get Ox blood plasma albumen, be dissolved in distilled water and make Ox blood plasma protein solution, after 90 ℃ of water-bath preheatings, regulating the pH of Ox blood plasma protein solution is 8.0, and temperature is 55 ℃;
Ii) in Ox blood plasma protein solution, add Sumizyme MP to start hydrolysis, in hydrolytic process, keep the pH value of solution constant, after hydrolysis, make enzyme-deactivating through 90 ℃ of water-baths, obtain hydrolyzed solution;
Iii) regulating step is after ii) the pH of gained hydrolyzed solution is 7.0, and vacuum lyophilization, obtains described Ox blood plasma proteolysate.
Step I i) in, the amount that adds Sumizyme MP is that to make the mass concentration ratio of basic protein enzyme-to-substrate be 1～2:100; Hydrolysis time is 120～150min.
A preparation method for the above-mentioned anti-oxidant wrapping material of edibility sunflower protein, comprises the following steps:
1) get sunflower protein and be dissolved in distilled water obtaining sunflower protein solution, then add glycerine, under room temperature, stir it is mixed, obtain mixed liquid B;
2) regulating the pH of mixed liquid B is 10～12, adds Ox blood plasma proteolysate, mixes, and obtains film-casting liquid;
3) by step 2) gained film-casting liquid sprawls masking, places after dry in moisture eliminator again, takes off film, to obtain final product.
In step 1), the concentration of described Ox blood plasma protein solution is 5%(w/v); In described mixed liquid B, the concentration of glycerine is 1%～2%(w/v).
In step 3), described dry temperature is 50～70 ℃, and the time is 4～6h; The relative humidity of described moisture eliminator is 58%, and temperature is 20～30 ℃; The time of placing in moisture eliminator is 48h.
By product when sunflower seed dregs is sunflower seeds liquefaction, wherein contains 29%～43% high-quality protein.Ox blood be about live body heavy 8%, in the blood plasma obtaining after separation, protein content is 6%～8%, is valuable protein resource.Plasma proteins has good gel property, emulsifying property and foaminess.Research shows, the proteolytic enzyme of application choice is at suitable Water Under solution protein, and its hydrolysate has stronger anti-oxidant activity.
The anti-oxidant wrapping material of edibility sunflower protein of the present invention, adopt Ox blood plasma proteolysate and sunflower protein to carry out composite, and gained edible packing material, has good physical strength, ultraviolet isolating performance and water vapor through performance; Ox blood plasma proteolysate is as a kind of natural antioxidants, edible packing material after interpolation is compared with simple sunflower protein film, oxidation-resistance is obviously strengthened, and can effectively prevent Food Oxidation corruption, thereby reaches the effect of food fresh keeping and Shelf-life; Gained wrapping material have edibility, can be biodegradable, can be to environment; Ox blood plasma proteolysate and sunflower protein, as natural biologic material, are renewable resourcess, wide material sources, and production cost is low, can replace other wrapping material, has greatly saved Nonrenewable resources, is applicable to applying.
The anti-oxidant wrapping material of edibility sunflower protein of the present invention, base material using the sunflower protein extracting from sunflower seed dregs as edible film, sunflower seed dregs is made the best use of everything, significantly promote the added value of sunflower seed dregs, be added in edible film Ox blood plasma proteolysate as natural antioxidants again, turn waste into wealth, for a new outlet is found in the comprehensive utilization of ox blood.
The preparation method of the anti-oxidant wrapping material of edibility sunflower protein of the present invention, after being made to film-casting liquid, sunflower protein and Ox blood plasma proteolysate sprawl masking, gained edibility oxidation-resistant film has good physical strength, ultraviolet isolating performance and water vapor through performance, can effectively prevent Food Oxidation corruption, thereby reach the effect of food fresh keeping and Shelf-life; Preparation method of the present invention, raw material sources are extensive, technique is simple, easy to operate, and production cost is low, is applicable to large-scale industrial production.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
In the specific embodiment of the present invention, the raw materials used commercial goods that is, sunflower seed dregs is purchased from Shandong Hao Zheng Farming Ltd.; Ox blood plasma albumen (powdery) is purchased from Tianjin En Bi protein company limited, and protein content is 70%; (vigor is 10 to Sumizyme MP 6u/g) be purchased from good and bio tech ltd, Shanghai.
Embodiment 1
The anti-oxidant wrapping material of edibility sunflower protein of the present embodiment, the component that comprises following parts by weight: 100 parts of sunflower proteins, 20 parts of Ox blood plasma proteolysates.
Described sunflower protein is prepared by following methods:
A) get sunflower seed dregs powder (described sunflower seed dregs powder is crossed 60 eye mesh screens and made after high speed Universalpulverizer is pulverized by sunflower seed dregs), according to solid-liquid ratio 1:15(w/v) ratio sunflower seed dregs powder is added in distilled water, stir 1h, adopting the NaOH solution adjusting pH that concentration is 3mol/L is 9, centrifugal 20min under 3500r/min speed conditions, obtains supernatant liquor and residue;
B) residue is extracted once by the method for step a) again, merge the supernatant liquor of extracted twice, obtain mixed liquor A;
C) adopt the hydrochloric acid that concentration is 3mol/L to regulate the pH of mixed liquor A to sunflower seed dregs albumen iso-electric point 4.5, stir 30min, there is Precipitation, centrifugal 20min under 3500r/min speed conditions, abandon supernatant liquor, after twice of distilled water wash that gained precipitation is 4.0 with pH, according to solid-liquid ratio 2:1(w/v) ratio add distilled water dissolving, obtain albumin milk;
D) by step c) gained albumin milk after twice homogeneous, the pressure of twice homogeneous is respectively 2 × 10 5pa and 5 × 10 5it is 9 that the NaOH solution that Pa is 3mol/L by concentration regulates the pH of albumin milk, and spray dry (temperature in is 170 ℃, and temperature out is 80 ℃), obtains described sunflower protein.
Described Ox blood plasma proteolysate is prepared by following methods:
I) get Ox blood plasma albumen, be dissolved in that in distilled water, to make concentration be 5%(w/w) Ox blood plasma protein solution, after 90 ℃ of water-bath preheating 10min, regulating the pH of Ox blood plasma protein solution is 8.0, temperature is 55 ℃;
Ii) to adding in Ox blood plasma protein solution Sumizyme MP to start hydrolysis, the amount that adds Sumizyme MP is that to make the mass concentration ratio of basic protein enzyme-to-substrate be 1:100; In hydrolytic process, constantly adding concentration is the NaOH solution of 1mol/L, keeps the pH value of hydrating solution constant, after hydrolysis 120min, makes enzyme-deactivating through 90 ℃ of water-bath 5min, obtains hydrolyzed solution;
Iii) the hydrochloric acid regulating step that is 1mol/L by concentration is after ii) the pH of gained hydrolyzed solution is 7.0, and vacuum lyophilization, obtains pulverulent solids, is described Ox blood plasma proteolysate.
By the sealing of gained Ox blood plasma proteolysate, at 4 ℃, preserve, for subsequent use.
The preparation method of the anti-oxidant wrapping material of edibility sunflower protein of the present embodiment, comprises the following steps:
1) getting sunflower protein is dissolved in distilled water to such an extent that concentration is 5%(w/v) sunflower protein solution, then add glycerine, under room temperature, stir 15min it mixed, obtain mixed liquid B; In described mixed liquid B, the concentration of glycerine is 1%(w/v);
2) it is 10 that the NaOH solution that is 2mol/L by concentration regulates the pH of mixed liquid B, stirs after 15min, adds Ox blood plasma proteolysate, mixes, and obtains film-casting liquid;
3) get 10ml step 2) gained film-casting liquid, spread into polystyrene plate (64cm 2) upper masking, after 60 ℃ of dry 5h, then be placed in that relative humidity (RH) is 58%, temperature is that the moisture eliminator of 20 ℃ is placed 48h, takes off film, obtains edibility sunflower protein oxidation-resistant film.
Embodiment 2
The anti-oxidant wrapping material of edibility sunflower protein of the present embodiment, the component that comprises following parts by weight: 100 parts of sunflower proteins, 25 parts of Ox blood plasma proteolysates.
Described sunflower protein is prepared by following methods:
A) get sunflower seed dregs powder (described sunflower seed dregs powder is crossed 60 eye mesh screens and made after high speed Universalpulverizer is pulverized by sunflower seed dregs), according to solid-liquid ratio 1:14(w/v) ratio sunflower seed dregs powder is added in distilled water, stir 0.9h, adopting the NaOH solution adjusting pH that concentration is 3mol/L is 8.5, centrifugal 25min under 3500r/min speed conditions, obtains supernatant liquor and residue;
B) residue is extracted once by the method for step a) again, merge the supernatant liquor of extracted twice, obtain mixed liquor A;
C) adopt the hydrochloric acid that concentration is 3mol/L to regulate the pH of mixed liquor A to sunflower seed dregs albumen iso-electric point 4.5, stir 35min, there is Precipitation, centrifugal 20min under 3500r/min speed conditions, abandon supernatant liquor, after twice of distilled water wash that gained precipitation is 4.0 with pH, according to solid-liquid ratio 2.2:1(w/v) ratio add distilled water dissolving, obtain albumin milk;
D) by step c) gained albumin milk after twice homogeneous, the pressure of twice homogeneous is respectively 2 × 10 5pa and 5 × 10 5it is 8.5 that the NaOH solution that Pa is 3mol/L by concentration regulates the pH of albumin milk, and spray dry (temperature in is 180 ℃, and temperature out is 85 ℃), obtains described sunflower protein.
Described Ox blood plasma proteolysate is prepared by following methods:
I) get Ox blood plasma albumen, be dissolved in that in distilled water, to make concentration be 5%(w/w) Ox blood plasma protein solution, after 90 ℃ of water-bath preheating 10min, regulating the pH of Ox blood plasma protein solution is 8.0, temperature is 55 ℃;
Ii) to adding in Ox blood plasma protein solution Sumizyme MP to start hydrolysis, the amount that adds Sumizyme MP is that to make the mass concentration ratio of basic protein enzyme-to-substrate be 1.5:100; In hydrolytic process, constantly adding concentration is the NaOH solution of 1mol/L, keeps the pH value of hydrating solution constant, after hydrolysis 135min, makes enzyme-deactivating through 90 ℃ of water-bath 5min, obtains hydrolyzed solution;
Iii) the hydrochloric acid regulating step that is 1mol/L by concentration is after ii) the pH of gained hydrolyzed solution is 7.0, and vacuum lyophilization, obtains pulverulent solids, is described Ox blood plasma proteolysate.
By the sealing of gained Ox blood plasma proteolysate, at 4 ℃, preserve, for subsequent use.
The preparation method of the anti-oxidant wrapping material of edibility sunflower protein of the present embodiment, comprises the following steps:
1) getting sunflower protein is dissolved in distilled water to such an extent that concentration is 5%(w/v) sunflower protein solution, then add glycerine, under room temperature, stir 15min it mixed, obtain mixed liquid B; In described mixed liquid B, the concentration of glycerine is 1.5%(w/v);
2) it is 11 that the NaOH solution that is 2mol/L by concentration regulates the pH of mixed liquid B, stirs after 15min, adds Ox blood plasma proteolysate, mixes, and obtains film-casting liquid;
3) get 10ml step 2) gained film-casting liquid, spread into polystyrene plate (64cm 2) upper masking, after 60 ℃ of dry 5h, then be placed in that relative humidity (RH) is 58%, temperature is that the moisture eliminator of 20 ℃ is placed 48h, takes off film, obtains edibility sunflower protein oxidation-resistant film.
Embodiment 3
The anti-oxidant wrapping material of edibility sunflower protein of the present embodiment, the component that comprises following parts by weight: 100 parts of sunflower proteins, 30 parts of Ox blood plasma proteolysates.
Described sunflower protein is prepared by following methods:
A) get sunflower seed dregs powder (described sunflower seed dregs powder is crossed 80 eye mesh screens and made after high speed Universalpulverizer is pulverized by sunflower seed dregs), according to solid-liquid ratio 1:16(w/v) ratio sunflower seed dregs powder is added in distilled water, stir 1.1h, adopting the NaOH solution adjusting pH that concentration is 3mol/L is 9.5, centrifugal 15min under 3500r/min speed conditions, obtains supernatant liquor and residue;
B) residue is extracted once by the method for step a) again, merge the supernatant liquor of extracted twice, obtain mixed liquor A;
C) adopt the hydrochloric acid that concentration is 3mol/L to regulate the pH of mixed liquor A to sunflower seed dregs albumen iso-electric point 4.5, stir 25min, there is Precipitation, centrifugal 20min under 3500r/min speed conditions, abandon supernatant liquor, after twice of distilled water wash that gained precipitation is 4.0 with pH, according to solid-liquid ratio 1.5:1(w/v) ratio add distilled water dissolving, obtain albumin milk;
D) by step c) gained albumin milk after twice homogeneous, the pressure of twice homogeneous is respectively 2 × 10 5pa and 5 × 10 5it is 9.5 that the NaOH solution that Pa is 3mol/L by concentration regulates the pH of albumin milk, and spray dry (temperature in is 190 ℃, and temperature out is 90 ℃), obtains described sunflower protein.
Described Ox blood plasma proteolysate is prepared by following methods:
I) get Ox blood plasma albumen, be dissolved in that in distilled water, to make concentration be 5%(w/w) Ox blood plasma protein solution, after 90 ℃ of water-bath preheating 10min, regulating the pH of Ox blood plasma protein solution is 8.0, temperature is 55 ℃;
Ii) to adding in Ox blood plasma protein solution Sumizyme MP to start hydrolysis, the amount that adds Sumizyme MP is that to make the mass concentration ratio of basic protein enzyme-to-substrate be 2:100; In hydrolytic process, constantly adding concentration is the NaOH solution of 1mol/L, keeps the pH value of hydrating solution constant, after hydrolysis 150min, makes enzyme-deactivating through 90 ℃ of water-bath 5min, obtains hydrolyzed solution;
Iii) the hydrochloric acid regulating step that is 1mol/L by concentration is after ii) the pH of gained hydrolyzed solution is 7.0, and vacuum lyophilization, obtains pulverulent solids, is described Ox blood plasma proteolysate.
By the sealing of gained Ox blood plasma proteolysate, at 4 ℃, preserve, for subsequent use.
The preparation method of the anti-oxidant wrapping material of edibility sunflower protein of the present embodiment, comprises the following steps:
1) getting sunflower protein is dissolved in distilled water to such an extent that concentration is 5%(w/v) sunflower protein solution, then add glycerine, under room temperature, stir 15min it mixed, obtain mixed liquid B; In described mixed liquid B, the concentration of glycerine is 2%(w/v);
2) it is 12 that the NaOH solution that is 2mol/L by concentration regulates the pH of mixed liquid B, stirs after 15min, adds Ox blood plasma proteolysate, mixes, and obtains film-casting liquid;
3) get 10ml step 2) gained film-casting liquid, spread into polystyrene plate (64cm 2) upper masking, after 60 ℃ of dry 5h, then be placed in that relative humidity (RH) is 58%, temperature is that the moisture eliminator of 20 ℃ is placed 48h, takes off film, obtains edibility sunflower protein oxidation-resistant film.
Experimental example 1
This experimental example is measured the traditional performance of embodiment 1～3 gained edibility oxidation-resistant film.
Traditional performance measuring method is as follows:
(1) measuring method of film thickness
Select intact, uniform film, get at random 5 points, measure its thickness with screw micrometer, average.
(2) film pierces through the mensuration of performance (PS)
Adopt matter structure instrument to measure, complete uniform membrane sample to be measured is placed between two homemade poly (methyl methacrylate) plates, wherein there is the circular hole of 3.2cm at poly (methyl methacrylate) plate center, and surrounding is fixed by screw.Select the probe of diameter 2mm, it is 1mm/s that probe moves down speed.Pierce through intensity (PS, N/mm) calculation formula as the formula (1).
PS = F p L - - - ( 1 )
In formula, F pfor maximum pierces through power (N); L is the thickness (mm) of membrane sample.
(3) mensuration of film tensile property (TS)
With sharp blade, film to be measured is cut into rectangular, and double faced adhesive tape is sticked to respectively in two ends in case in drawing process from fracture of root, final effectively tensile elongation is 40 × 6mm.The stretching device that uses matter structure instrument, draw speed is 1mm/s.Tensile strength (TS, Mpa) can obtain from pressing force-strain curve to calculate, and sees formula (2).
TS = F t L × W - - - ( 2 )
In formula, Ft is maximum pull (N); L is the thickness (mm) of membrane sample; W is the width (being 6mm) of membrane sample.
4) mensuration of film water vapor transmission (WVP)
Adopt and intend cup method, by complete uniform film phonograph seal, on the self-control synthetic glass moisture vapor transmission cup surface of containing 5g Calcium Chloride Powder Anhydrous (0%RH), film exposed area is 19.6cm 2.Moisture vapor transmission cup device is put into climatic chamber (80%RH, 25 ℃), measured the gain in weight of moisture vapor transmission cup every 24 hours, see formula (3).
WVP ( g · mm / m 2 · d · KPa ) = W × x A × T × ΔP - - - ( 3 )
In formula, the weightening finish (g) that W is moisture vapor transmission cup; X is the thickness (mm) of film; A is that film exposed area (is 19.6cm 2); T is interval time of measurement (being 24h); △ P is the water vapour pressure reduction (Δ P=2.5347KPa) of film both sides.
(5) mensuration of protein film moisture content (MC)
Protein film is cut into size to fit, place it in and in aluminium box, claim its weight; Be placed in 105 ℃ of baking ovens and dry 8h, after taking out, claim its weight, before and after drying according to film, changes in weight is calculated protein film equilibrium moisture content, sees formula (4).
S ( % ) = m 1 - m 2 m 1 × 100 - - - ( 4 )
In formula, Δ m is changes in weight before and after film is dried, m 0for film is dried front weight.
(6) mensuration of protein film solvability (S)
Taking quality is m 1the dry film of (≈ 0.1g) is put into the culture dish that fills 40ml water, film is pulled after dissolving 24h at 25 ℃, blots the moisture on film surface, then be dried to constant weight with filter paper under the condition of 105 ℃, claims to such an extent that quality is m 2, calculate protein film solvability (S) according to changes in weight before and after film, see formula (5).
S ( % ) = m 1 - m 2 m 1 × 100 - - - ( 5 )
The performance measurement result of embodiment 1～3 gained edibility oxidation-resistant film is as shown in table 1.
The performance measurement result of table 1 embodiment 1～3 gained edibility oxidation-resistant film
Figure BDA0000452615610000084
Experimental example 2
This experimental example is measured the antioxygen property of embodiment 1～3 gained edibility oxidation-resistant film.
Measuring method is as follows:
(1) preparation of sample solution
Taking quality is m 1the dry film of (≈ 0.1g) is put into the Erlenmeyer flask that fills 40ml water, and room temperature is put into shaking table, and rotating speed is 1000rpm jolting 24h, dissolves to urge it, then filters with filter paper, gets supernatant liquor and measures.
(2) FRAP (ferric reducing-antioxidant power, iron reduction resistance of oxidation) method is measured resistance of oxidation
In reaction tubes, add 0.1ml sample, more accurately add 3ml FRAP working fluid, 300 μ l distilled waters, mix, and accurate response 8min, reads absorbance in 593nm place, distilled water zeroing.With 100～1000 μ mol/L FeSO 47H 2o production standard curve.The anti-oxidant activity (FRAP value) of sample is to reach the required FeSO of same absorbance 4mmole number represents, every part of sample replication 3 times.FRAP working fluid matching while using, by 100ml30mmol/L acetate buffer, 10ml10mmol/LTPTZ solution and the 10ml20mmol/L FeCl of pH3.6 36H 2o solution mixes composition.
(3) mensuration of anti peroxidation of lipid ability
Liposome is made by soybean lecithin, and soybean lecithin is dissolved to (200mg/L) after 0.12mol/L KCl, 5mmol/L Histidine buffered soln (pH value 6.8) homogeneous, carries out 45min ultrasonication at 4 ℃, to urge its dissolving.
By 5ml liposome solutions and 1ml sample mix; Contrast is for replacing 1ml sample to mix with 5ml lipid with 1ml distilled water.Add 0.1ml50mmol/L FeCl 3cause lipid oxidation with 0.1ml10mmol/L sodium ascorbate, then sample is placed in 37 ℃ of water-baths and is incubated 1h, its degree of oxidation is measured by thiobarbituricacidα-(TBA) method.After sample solution reacts with 0.02mol/LTBA, with chloroform 1:1(v/v) mix vortex vibration, the centrifugal 10min of 1800g.Get the light absorption value that supernatant is determined at 532nm place.The calculation formula of lipid oxidation inhibiting rate is shown in to formula (6).
To lipid oxidation inhibiting rate (%)=[1-(light absorption value/contrast solution light absorption value of sample)] × 100% (6)
The antioxygen property measurement result of embodiment 1～3 gained edibility oxidation-resistant film is as shown in table 2.
Wherein, comparative example is the sunflower protein film that does not add Ox blood plasma proteolysate; Its preparation method is with embodiment 1, but in film-casting liquid, do not add Ox blood plasma proteolysate.
The antioxygen property measurement result of table 2 embodiment 1～3 gained edibility oxidation-resistant film
Figure BDA0000452615610000091

Claims (10)

1. the anti-oxidant wrapping material of edibility sunflower protein, is characterized in that: the component that comprises following parts by weight: 100 parts of sunflower proteins, 20～30 parts of Ox blood plasma proteolysates.
2. the anti-oxidant wrapping material of edibility sunflower protein according to claim 1, is characterized in that: described sunflower protein is prepared by following methods:
A) get sunflower seed dregs powder, according to the ratio of solid-liquid ratio 1:13～17, sunflower seed dregs powder is added in distilled water, stir, regulating pH is 8.5～9.5, centrifugal supernatant liquor and the residue of obtaining;
B) residue is extracted once by the method for step a) again, merge the supernatant liquor of extracted twice, obtain mixed liquor A;
C) regulate the pH of mixed liquor A to sunflower seed dregs albumen iso-electric point 4.5, stirring is also centrifugal, abandons supernatant liquor, and gained precipitates with after distilled water wash, adds distilled water to dissolve according to the ratio of solid-liquid ratio 1.5～2.2:1, obtains albumin milk;
D) by step c) gained albumin milk after twice homogeneous, regulate pH be 8.5～9.5, spray be dried, obtain described sunflower protein.
3. the anti-oxidant wrapping material of edibility sunflower protein according to claim 2, is characterized in that: the method that regulates pH in step a) and step d) is that to add concentration be the sodium hydroxide solution of 3mol/L; The method that regulates the pH of mixed liquor A in step c) is that to add concentration be the hydrochloric acid of 3mol/L.
4. the anti-oxidant wrapping material of edibility sunflower protein according to claim 2, is characterized in that: described in step a), centrifugal rotating speed is 3500r/min, the time is 15～25min; ; Described in step c), centrifugal rotating speed is 3500r/min, and the time is 20min.
5. the anti-oxidant wrapping material of edibility sunflower protein according to claim 2, is characterized in that: in step d), the pressure of described twice homogeneous is respectively 2 × 10 5pa, 5 × 10 5pa; Described spray-dired temperature in is 170～190 ℃, and temperature out is 80～90 ℃.
6. the anti-oxidant wrapping material of edibility sunflower protein according to claim 1, is characterized in that: described Ox blood plasma proteolysate is prepared by following methods:
I) get Ox blood plasma albumen, be dissolved in distilled water and make Ox blood plasma protein solution, after 90 ℃ of water-bath preheatings, regulating the pH of Ox blood plasma protein solution is 8.0, and temperature is 55 ℃;
Ii) in Ox blood plasma protein solution, add Sumizyme MP to start hydrolysis, in hydrolytic process, keep the pH value of solution constant, after hydrolysis, make enzyme-deactivating through 90 ℃ of water-baths, obtain hydrolyzed solution;
Iii) regulating step is after ii) the pH of gained hydrolyzed solution is 7.0, and vacuum lyophilization, obtains described Ox blood plasma proteolysate.
7. the anti-oxidant wrapping material of edibility sunflower protein according to claim 6, is characterized in that: step I i) in, the amount that adds Sumizyme MP is that to make the mass concentration ratio of basic protein enzyme-to-substrate be 1～2:100; Hydrolysis time is 120～150min.
8. a preparation method for the anti-oxidant wrapping material of edibility sunflower protein as claimed in claim 1, is characterized in that: comprise the following steps:
1) get sunflower protein and be dissolved in distilled water obtaining sunflower protein solution, then add glycerine, under room temperature, stir it is mixed, obtain mixed liquid B;
2) regulating the pH of mixed liquid B is 10～12, adds Ox blood plasma proteolysate, mixes, and obtains film-casting liquid;
3) by step 2) gained film-casting liquid sprawls masking, places after dry in moisture eliminator again, takes off film, to obtain final product.
9. the preparation method of the anti-oxidant wrapping material of edibility sunflower protein according to claim 8, is characterized in that: in step 1), the concentration of described Ox blood plasma protein solution is 5%(w/v); In described mixed liquid B, the concentration of glycerine is 1%～2%(w/v).
10. the preparation method of the anti-oxidant wrapping material of edibility sunflower protein according to claim 8, is characterized in that: in step 3), described dry temperature is 50～70 ℃, and the time is 4～6h; The relative humidity of described moisture eliminator is 58%, and temperature is 20～30 ℃; The time of placing in moisture eliminator is 48h.
CN201410001166.5A 2014-01-02 2014-01-02 Anti-oxidant wrapping material of a kind of edibility sunflower protein and preparation method thereof Expired - Fee Related CN103773002B (en)

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Publication number Priority date Publication date Assignee Title
CN107619606A (en) * 2017-10-31 2018-01-23 南京财经大学 A kind of edibility rapeseed protein composite membrane

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CN1095033A (en) * 1993-05-11 1994-11-16 符玉华 Edible film packing material
CN101580595A (en) * 2009-06-18 2009-11-18 沈阳农业大学 Method for preparing edible composite protein film
CN101851424A (en) * 2009-03-31 2010-10-06 西南科技大学 Thermoplastic plant protein/polylactic acid blend material and preparation method thereof
CN102191304A (en) * 2011-03-23 2011-09-21 连云港福润食品有限公司 Porcine plasma antioxidant peptide

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CN1095033A (en) * 1993-05-11 1994-11-16 符玉华 Edible film packing material
CN101851424A (en) * 2009-03-31 2010-10-06 西南科技大学 Thermoplastic plant protein/polylactic acid blend material and preparation method thereof
CN101580595A (en) * 2009-06-18 2009-11-18 沈阳农业大学 Method for preparing edible composite protein film
CN102191304A (en) * 2011-03-23 2011-09-21 连云港福润食品有限公司 Porcine plasma antioxidant peptide

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107619606A (en) * 2017-10-31 2018-01-23 南京财经大学 A kind of edibility rapeseed protein composite membrane

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