CN103768168A - Extraction method for r ranunculus ternatus thunb tannin - Google Patents

Abstract

The invention provides an extraction method for ranunculus ternatus thunb tannin. The extraction method comprises the following steps: first, a dry ranunculus ternatus thunb medicinal material is crushed; second, the powder obtained from the first step is soaked with petroleum ether to remove grease and pigments; third, filtration is carried out, the filter residue is obtained, dried and added with 40%-50% of ethanol solution, supersonic extraction is carried out for 1-3 times, filtration is carried out, the extraction solutions of multiple extractions are mixed; fourth, the mixed extraction solution is subjected to concentration until there is no ethanol smell, clear water is added, supersonic dissolving is carried out until the maximum solubility is reached, filtration is carried out, an excess 4% gelatin aqueous solution is added to the filtrate until precipitation no longer increases, the precipitation is obtained through filtration and dried; fifth, the dried solid powder obtained from the fourth step is subjected to soxhlet extraction with acetone until there is no response to ferric trichloride developing, acetone is recycled and the tannin component is obtained. The extraction method is advantageous in that, the effects of the pharmacological ingredients are strong, the technology is simple and the method is suitable for industrial utilization.

Description

A kind of extracting method of Radix Ranunculi Ternati tannin

Technical field

The present invention relates to natural product extracting technique field, relate in particular to a kind of extracting method of Radix Ranunculi Ternati tannin.

Background technology

Tannin is extensively present in plant kingdom, is present in the positions such as the skin, wood, leaf, root, fruit of plant, approximately in more than 70% Chinese medicinal plant medicine, contains tannins; Nearly ten years, due to new technique, the application of new method, people make substantial progress to the research of tannin in plant, except finding that it has parasite killing, antibacterial, antiinflammatory, hemostasis pharmacologically active, also find to there is the multiple pharmacologically actives such as mutation, anti peroxidation of lipid, removing free radical, antitumor and anti-AIDS, especially in antineoplaston, demonstrated tempting prospect.

Radix Ranunculi Ternati is the tuber of cohosh ranunculus ternatus, its tuber meat, several clustering, nearly spindle, crust yellowish-brown, likeness in form ram's horn, main product is in various places, the middle and lower reach of Yangtze River, generally slightly poisonous, there is effect of detoxicating and resolving stagnation of pathogens, general as drawing red foaming, applying acupuncture point, can treat various diseases, also can parasite killing, modern pharmacology and clinical research show Radix Ranunculi Ternati and preparation effective in cure to pulmonary tuberculosis, lymphoid tuberculosis and kinds cancer and lymphoma.

Summary of the invention

In view of above-mentioned prior art, the object of the invention is to propose a kind of extracting method of Radix Ranunculi Ternati tannin, make the specific aim of pharmacological action stronger, better effects if, is suitable for suitability for industrialized production.

Object of the present invention will be achieved by the following technical programs:

An extracting method for Radix Ranunculi Ternati tannin, comprises the steps:

Step 1, is ground into by dry Radix Ranunculi Ternati medical material the powder that at least can cross No. 1 sieve;

Step 2, the powder of use petroleum ether soaking step one gained, consumption is 2～3 times of powder volume, soak time is 2～4 hours, removes oils and fats and color element;

Step 3, filters, and leaching filtering residue is dry, adds 40%～50% alcoholic solution, supersound extraction 1～3 time, and each 1～3 hour is 2～4 times of filtering residue volume by alcoholic solution amount at every turn, filters and merges the extracting solution repeatedly extracting;

Step 4, concentrated extracting solution to without alcohol taste, adds clear water ultrasonic dissolution, to maxima solubility, filters, and adds excessive 4% aqueous gelatin solution in filtrate, no longer increase to precipitation capacity, leaching precipitation, dry;

Step 5, using acetone to carry out Soxhlet to the drying solid powder of step 4 gained, to be extracted into ferric chloride colour developing reactionless, reclaims acetone and obtain tannin composition.

Outstanding effect of the present invention is: propose a kind of extracting method of Radix Ranunculi Ternati tannin, make the effect of pharmaceutical component stronger, technique is simple, is more suitable for industrialization and uses.

The specific embodiment

Below by specific embodiment, method of the present invention is described, but the present invention is not limited thereto.Experimental technique described in following embodiment, if no special instructions, is conventional method; Described reagent and material, if no special instructions, all can obtain from commercial channels.

Embodiment:

The extracting method of a kind of Radix Ranunculi Ternati tannin of the present embodiment, comprises the steps:

Step 1, is ground into by dry Radix Ranunculi Ternati medical material the powder that at least can cross No. 1 sieve;

Step 2, the powder of use petroleum ether soaking step one gained, consumption is 2～3 times of powder volume, soak time is 2～4 hours, removes oils and fats and color element;

Step 3, filters, and leaching filtering residue is dry, adds 40%～50% alcoholic solution, supersound extraction 1～3 time, and each 1～3 hour is 2～4 times of filtering residue volume by alcoholic solution amount at every turn, filters and merges the extracting solution repeatedly extracting;

Step 4, concentrated extracting solution to without alcohol taste, adds clear water ultrasonic dissolution, to maxima solubility, filters, and adds excessive 4% aqueous gelatin solution in filtrate, no longer increase to precipitation capacity, leaching precipitation, dry;

Step 5, using acetone to carry out Soxhlet to the drying solid powder of step 4 gained, to be extracted into ferric chloride colour developing reactionless, reclaims acetone and obtain tannin composition.

The outstanding effect of the present embodiment is: propose a kind of extracting method of Radix Ranunculi Ternati tannin, make the effect of pharmaceutical component stronger, technique is simple, is more suitable for industrialization and uses.

The present invention still has numerous embodiments, and all employing equivalents or equivalent transformation and all technical schemes of forming, within all dropping on protection scope of the present invention.

Claims (1)

1. an extracting method for Radix Ranunculi Ternati tannin, is characterized in that comprising the steps:

Step 1, is ground into by dry Radix Ranunculi Ternati medical material the powder that at least can cross No. 1 sieve;

Step 2, the powder of use petroleum ether soaking step one gained, consumption is 2～3 times of powder volume, soak time is 2～4 hours;

Step 3, filters, and leaching filtering residue is dry, adds 40%～50% alcoholic solution, supersound extraction 1～3 time, and each 1～3 hour is 2～4 times of filtering residue volume by alcoholic solution amount at every turn, filters and merges the extracting solution repeatedly extracting;

Step 4, concentrated extracting solution to without alcohol taste, adds clear water ultrasonic dissolution, to maxima solubility, filters, and adds excessive 4% aqueous gelatin solution in filtrate, no longer increase to precipitation capacity, leaching precipitation, dry;

Step 5, using acetone to carry out Soxhlet to the drying solid powder of step 4 gained, to be extracted into ferric chloride colour developing reactionless, reclaims acetone and obtain tannin composition.