CN103766531A - Method for preparing high gamma-aminobutyric acid instant tea - Google Patents
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Abstract
The invention discloses a method for preparing high gamma-aminobutyric acid instant tea. The method comprises the following steps: soaking fresh tea leaves into a 0.25% to 2.5% glutamic acid aqueous solution, the weight of which is one to four times that of the fresh tea leaves; performing anaerobic treatment on the tea leaves for 0.5 to 30 hours under the vacuum degree being -0.05mpa to -0.1mpa; taking out the tea leaves and putting the tea leaves into boiling water, the weight of which is one to four times that of the tea leaves, for 10 to 30 seconds; combining the tea leaves with the glutamic acid aqueous solution after being used for soaking the fresh tea leaves; crushing and homogenizing for 1 to 5 minutes; filtering with a filter screen being 200 meshes; performing high-speed centrifugation on a filtrate at 5000rpm to 7000rpm (Revolutions Per Minute); performing reverse osmosis concentration until the concentration is greater than or equal to 100g/L; then performing reduced pressure evaporation concentration until the concentration is greater than or equal to 300g/L; and performing spray drying or freezing drying until the water content is less than or equal to 5%, thereby obtaining the high gamma-aminobutyric acid instant tea in a shape of green powder. The content of gamma-aminobutyric acid in gamma-aminobutyric acid is between 20.5mg/g and 35.5mg/g.
Description
Technical field
The present invention relates to a kind of preparation method of instant tea powder, be specially one and prepare the high gamma-aminobutyric acid instant tea method of (claiming again GABA instant tea).
Background technology
GABA (GABA) is a kind of nonprotein amino acid, is present in animal, plant and microorganism.In mammal, GABA is present in brain and spinal cord, it is the inhibitory neurotransmitter of central nervous system, having calm calm the nerves, reduce blood pressure, treat epilepsy with anticonvulsion, improve brain function, promote growth hormone secretion, promote to sleep, delay senility, strengthen long-term memory and raising liver, the physiologically active such as kidney function, can be applicable to the fields such as medicine, food, beverage, cosmetics.The content of the GABA in ordinary tea leaves is very low, generally between 0.02～0.2mg/g, and more than its content of gamma-aminobutyric acid tea (GABA tea) General Requirements reaches 1.5mg/g.
GABA in tealeaves is mainly produced through decarboxylic reaction by glutamic acid, and this reaction is by glutamate decarboxylase catalysis.Therefore the glutamic acid in tealeaves is the main precursor substance of GABA.Also there are some researches show, GABA is relevant in the stress reaction under adverse environmental factor with plant.In anoxic, damage to plants caused by sudden drop in temperature, under the multiple adverse environmental factor such as thermostimulation, mechanical stimulus, arid, salt stress, the alpha-aminobutyric acid content in plant increases.The processing method of at present conventional GABA tealeaves processing has:
1, be sprayed at tea tree with glutamic acid aqueous solution: to adopt mass concentration be 0.1%～1.0%, and glutamic acid aqueous solution is sprayed on fresh leaves of tea plant, the fresh leaves of tea plant of gathering after 3-7 days be raw material for processing GABA tea, the GABA content of product is 3.53-4.80mg/g[1].
2, use the sodium glutamate immersion 4h of 0.5-3.5% concentration to the betel of water content 68% by spreading to wither, rinse and remove the remaining sodium glutamate of leaf surface with clear water, then be placed in vacuum drying chamber Anaerobic Treatment 8h, the GABA content of product is 1.575-2.501mg/g[2].
3, in order to improve vacuum Anaerobic Treatment effect, after vacuumizing, be filled with nitrogen or carbon dioxide.The product G ABA content of processing is 1.7647-3.3297mg/g[3].The GABA content of vacuum nitrogen and soaked with liquid processing reaches respectively 2.88mg/g and 4.01mg/g[4].
4, the glutamic acid solution dipping 5.5h for the treatment of conditions optimization: draw after glutamic acid solution concentration, pH and dip time are optimized to screening, the condition of optimization is: pH4.8, the GABA content of product reaches 4.5461mg/g[5].
5, GABA instant tea powder processing: fresh leaves of tea plant is processed to 2-6h at 0.04-0.1pa vacuum, 15-30 ℃, then by tealeaves successively 100-120 ℃ and 80-100 ℃ of oven dry, gained tealeaves is after dressing sieve destemming etc. is processed, extract 15-35min at 50-80 ℃, extract is through filtering, concentrate and being dried, can obtain GABA instant tea powder, the alpha-aminobutyric acid content of product is 10-19mg/g[6].
Existing GABA production of instant tea method be the dry tea of GABA tealeaves of preparing be especially raw material, by water extraction get, filter, concentrated and spraying or freeze drying, make pulverous GABA instant tea powder.Preparing the dry tea time of GABA tealeaves, by glutamic acid or sodium glutamate water-soluble sprinkling tea tree or dipping tealeaves, then fresh leaves of tea plant is filled to nitrogen or the processing of filling CO 2 condition through vacuum, vacuum person, impel GABA concentration to improve, then be processed into the dry tea of GABA tealeaves through operations such as kneading, be dried, can directly brew tea and drink, also can be used as the raw material of processing GABA instant tea.
1), need to be by tea-drying while producing the dry tea raw material of GABA tealeaves the problem that existing method of producing GABA instant tea take the dry tea of GABA tealeaves as raw material exists has:, while producing GABA instant tea, again stem tea is immersed in to hot water and extracts, energy consumption is large; 2) fresh leaves of tea plant is immersed in to glutamic acid aqueous solution processing and can improves alpha-aminobutyric acid content, but the GABA that has part to generate in this processing procedure has dissolved and has entered the aqueous solution, and these aqueous solution are as discharge of wastewater, both be unfavorable for environmental protection, the materials such as GABA wherein also can not get recycling, the wasting of resources, increases cost.
The list of references above relating to is specific as follows:
1. fixed, Tang Chaqin, Chen Xuan, Xu Deliang, Xiao Runlin, Li Xinghui. the impact of Foliar Spraying Amino Acid on content of gamma-aminobutyric acid in tea leaves.Tea science, 2006,26 (4): 237-242.
2. mao clear multitude, Dai little Mei, Yang Xin river, Mu Rubao. vacuum and external source sodium glutamate are processed gamma-aminobutyric acid in tea leaves enrichment research .Food and Machinery, 2009,25 (4): 49-51.
3. woods intelligence, Lin Zhongming, Yin Junfeng, Tan Junfeng. the impact of Anaerobic Treatment on content of gamma-aminobutyric acid in tea leaves and quality thereof. Food Science, 2004,25(2): 35-39.
4. yellow sub-brightness, Chen Jianhua, Zeng Zhen, Zheng Hongfa, Huang Huaisheng. improve the method research of content of gamma-aminobutyric acid in tea leaves. Chinese agronomy circular, 2010,26 (11): 236-240.
5. Liao star. the glutamic acid treatment conditions of tealeaves enrichment GABA are optimized .Food and Machinery, 2011,27 (3): 123-126.
6. woods intelligence, Tan Junfeng, Xu Yongquan, Yin Junfeng, Lv Haipeng, Guo Li. a kind of processing method of gamma-aminobutyric acid tea powder. national inventing patent, ZL201110007686.3.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of method of preparing high gamma-aminobutyric acid instant tea, adopt the method preparation and instant tea in the content >=20.5mg/g of GABA.
The present invention directly prepares high gamma-aminobutyric acid instant tea take fresh leaves of tea plant flood through glutamic acid aqueous solution and carry out vacuum Anaerobic Treatment as raw material extracts, the energy consumption of minimizing tea-drying process; While recycles the aqueous solution after the fresh leaf of dipping tea at leaching process, reduces discharge of wastewater, the utilization rate of the compositions such as raising GABA wherein, the alpha-aminobutyric acid content of increase GABA instant tea.The present invention soaks by glutamic acid aqueous solution and application of vacuum fresh leaves of tea plant promotes generation and the accumulation of gamma-aminobutyric acid in tea leaves, then by after processing containing the fresh tea passes of GABA in boiling water, soak, pulverize homogenate, high gamma-aminobutyric acid instant tea product is prepared in extraction.
In order to solve the problems of the technologies described above, the invention provides a kind of method of preparing high gamma-aminobutyric acid instant tea, select at least one in the fresh leaves of tea plant of two leaves and a bud～mono-bud five leaves as tea raw material, carry out successively following processing step:
(1), fresh leaves of tea plant is immersed in the glutamic acid aqueous solution that the mass concentration of 1～4 times of fresh leaves of tea plant weight is 0.25%～2.5%, thus fresh leaves of tea plant is drenched;
Remarks explanation: as long as this immersion makes fresh leaves of tea plant by fully drenched, that is, general only need soaked 3～5 minutes;
(2), by tealeaves (, comprising the fresh leaves of tea plant+glutamic acid aqueous solution after the immersion) Anaerobic Treatment 0.5～30h under-0.05～-0.1mpa vacuum degree condition in glutamic acid aqueous solution that is immersed in of above-mentioned steps (1) gained; Separate, thus respectively glutamic acid aqueous solution after tealeaves and vacuum Anaerobic Treatment after vacuum Anaerobic Treatment;
(3), tealeaves after vacuum Anaerobic Treatment is put into refiner, add the boiling water of 1～4 times of step (1) fresh leaves of tea plant weight to soak for 10～30 seconds (thereby substituting traditional de-enzyming process);
Again glutamic acid aqueous solution after vacuum Anaerobic Treatment is poured into (object is to recycle) in refiner, opened refiner (by tealeaves) and pulverize homogenate 1-5 minute; Obtain homogenate;
(4), homogenate is filtered and removed tea grounds by the filter of 200 mesh filter screens, by the filtered fluid obtaining by the high speed centrifugation of 5000-7000rpm, get the supernatant of gained after high speed centrifugation and carry out the reverse osmosis concentrated concentration >=100g/L that is reduced to, reduction vaporization is concentrated into concentration >=300g/L again, process the % by weight to water content≤5%(finally by being dried), obtain high gamma-aminobutyric acid instant tea.
Remarks explanation: the immersion of step (1) can be carried out in rustless steel container.The Anaerobic Treatment of step (2) can be carried out in vacuum drying chamber.
Improvement as the method for preparing high gamma-aminobutyric acid instant tea of the present invention:
In described step (4),
The concentrated technological parameter of reduction vaporization is: temperature is 40～60 ℃, and vacuum is-0.05～-0.1mpa;
Described dry be treated to spray dry or freeze drying.
In the present invention,
Rely in step (1) and (2), can make glutamic acid molecule infiltration fully contact with Pidolidone decarboxylase to tea leaf is inner, promote glutamic acid decarboxylic reaction to produce GABA, thereby improve the content of gamma-aminobutyric acid in tea leaves.
Rely on the immersion treatment that adds boiling water of step (3), can substitute traditional tea green-keeping technique, because the air heat transfer that heat transfer water completes than tradition is quicker and efficient, therefore can realize the object completing fast, fixation time shortens to 10-30 second by traditional roller fixation 8-10 minute.
Rely on the refiner that glutamic acid aqueous solution after vacuum Anaerobic Treatment is poured in step (3) recycle and open refiner tealeaves is pulverized to homogenate 1-5 minute; Thereby realize: utilize the agitator while pulverizing the alternative traditional handicraft extraction of homogenate tealeaves to stir, tealeaves is extracted more thorough; Extraction time also reduced to 1-5 minute by 30-40 minute of traditional handicraft, saved time.
In step (4), homogenate is filtered and is removed tea grounds by the filter of 200 mesh filter screens, filtrate is removed to solids precipitation thing and other impurity by the high speed centrifugation of 5000-7000rpm, get the supernatant of gained after high speed centrifugation and carry out the reverse osmosis concentrated concentration >=100g/L that is reduced to, be that 40～60 ℃ and vacuum-0.05～-0.1mpa reduction vaporization are concentrated into concentration >=300g/L in temperature again, finally spraying is dry, intake air temperature 180-190 ℃, exhaust outlet temperature 80-90 ℃, the high gamma-aminobutyric acid instant tea powder of acquisition water content≤5%; Also can be by freeze drying to water content≤5%, thus high gamma-aminobutyric acid instant tea powder obtained.
Inventive point of the present invention is mainly:
1) common instant tea is raw materials used is dry tea, and the present invention is raw materials used is the fresh leaves of tea plant after vacuum Anaerobic Treatment;
2) the present invention's boiling water amount used in preparation process is far smaller than the required boiling water amount of existing common instant tea;
3) common instant tea leaching process uses agitator to stir, and homogenate pulverized by tealeaves by the present invention, makes the extraction of tealeaves more abundant.
The method of preparing high gamma-aminobutyric acid instant tea of the present invention, fresh leaves of tea plant is immersed in to glutamic acid and carries out vacuum Anaerobic Treatment (vacuumizing reduced pressure treatment) in water-soluble, the space segment that makes glutamic acid aqueous solution enter tea leaf inside is replaced air wherein, allow more glutamic acid molecule contact with the Pidolidone decarboxylase of blade interior, promote glutamic acid decarboxylic reaction to produce GABA, improve the content of gamma-aminobutyric acid in tea leaves.
The method of preparing high gamma-aminobutyric acid instant tea of the present invention, directly the fresh leaves of tea plant of vacuum decompression processing being put into boiling water soaks, substitute traditional tea green-keeping technique, the air completing higher than tradition because of speed and the efficiency of heat transfer water conducts heat, and can realize fast and completing.After completing step 2) produce the aqueous solution that contains GABA (, glutamic acid aqueous solution after vacuum Anaerobic Treatment) pour refiner into and contact with tealeaves with hot water wherein, reduce water temperature, increase the water yield, then tealeaves homogenate is pulverized, reach the object of resource high-efficiency extraction and application.This process realized simultaneously traditional handicraft tea green-keeping, knead, be dried, the target of the operation such as extraction, have energy consumption low, reduce discharge of wastewater, resource utilization advantages of higher.Meanwhile, because reducing the destruction of tea-drying process to chlorophyll and catechin compounds, the color and luster of product is greener, and exterior quality obviously improves.The alpha-aminobutyric acid content of high gamma-aminobutyric acid instant tea product prepared by the present invention is up to 20.5-35.5mg/g.
In sum, fresh leaves of tea plant is immersed in glutamic acid solution by the present invention, under-0.05～-0.1mpa vacuum degree condition, processes tealeaves 0.5-30 hour, improves the GABA level of tealeaves; Tealeaves soaks and completes, pulverizes that homogenate is extracted, filtered, concentrated, spraying is dry or the step such as freeze drying is made high gamma-aminobutyric acid instant tea powder through superheated water (boiling water) again.The present invention impels glutamic acid aqueous solution to enter tea leaf inside, allows more glutamic acid molecule contact with the glutamate decarboxylase of blade interior, promotes glutamic acid decarboxylic reaction to produce more GABA; The present invention by hot-water soak complete, recycling step 2) process the aqueous solution after tea fresh leaf, pulverize homogenate and extract, realized simultaneously traditional handicraft tea green-keeping, knead, be dried, the function of the operation such as extraction, have that energy consumption is low, discharge of wastewater is few, resource utilization advantages of higher; The alpha-aminobutyric acid content of product is apparently higher than conventional art.
The specific embodiment
Embodiment 1, a kind of method of preparing high gamma-aminobutyric acid instant tea, select the fresh leaves of tea plant (tea tree breed " the ancient tea of water ") of two leaves and a bud as tea raw material, to carry out successively following processing step:
1), the fresh leaves of tea plant of 1000g two leaves and a bud is put into the Stainless steel basin of diameter 35cm, degree of depth 20cm, adding mass concentration is 2.5% glutamic acid aqueous solution 1000g, makes tealeaves evenly drenched;
2), by above-mentioned steps 1) tealeaves (that is, comprising the fresh leaves of tea plant+glutamic acid aqueous solution after immersion) being immersed in glutamic acid aqueous solution of gained is placed in vacuum drying chamber together with rustless steel container, Anaerobic Treatment 30h under-0.1mpa vacuum degree condition; Separate, thus respectively glutamic acid aqueous solution after tealeaves and vacuum Anaerobic Treatment after vacuum Anaerobic Treatment;
3), tealeaves after vacuum Anaerobic Treatment is put into refiner, add 2000g boiling water to soak for 10 seconds (thereby substituting traditional de-enzyming process);
Again glutamic acid aqueous solution after vacuum Anaerobic Treatment is poured into (object is to recycle) in refiner, opened refiner tealeaves is pulverized to homogenate 5 minutes; Obtain homogenate;
4), homogenate is filtered and removed tea grounds by the filter of 200 mesh filter screens, by the filtered fluid obtaining by the high speed centrifugation of 7000rpm, getting after high speed centrifugation the supernatant of gained, to carry out the reverse osmosis concentrated concentration that is reduced to be 100g/L, concentrated (temperature is 40～60 ℃ to reduction vaporization again, vacuum is-0.05～-0.1mpa) to concentration be 400g/L, last freeze drying, to water content≤5%, obtains high gamma-aminobutyric acid instant tea powder.
High gamma-aminobutyric acid instant tea prepared by this law is green powder, detects the alpha-aminobutyric acid content 35.5mg/g of product through HPLC.
Embodiment 2, a kind of method of preparing high gamma-aminobutyric acid instant tea, select fresh leaves of tea plant (tea tree breed " Fuding white tea ", two leaves and a bud 50%, bud three leaves 50%) as tea raw material, to carry out successively following processing step:
1), above-mentioned 1000g fresh leaves of tea plant is put into the Stainless steel basin of diameter 45cm, degree of depth 30cm, adding mass concentration is 0.25% glutamic acid aqueous solution 4000g, makes tealeaves evenly drenched;
2), by above-mentioned steps 1) tealeaves that is immersed in glutamic acid aqueous solution of gained is placed in vacuum drying chamber together with rustless steel container, Anaerobic Treatment 0.5h under-0.05ma vacuum degree condition; Separate, thus respectively glutamic acid aqueous solution after tealeaves and vacuum Anaerobic Treatment after vacuum Anaerobic Treatment;
3), the tealeaves after vacuum Anaerobic Treatment is put into refiner, add 1000g boiling water to soak for 30 seconds (thereby substituting traditional de-enzyming process);
Again glutamic acid aqueous solution after vacuum Anaerobic Treatment is poured into (object is to recycle) in refiner, opened refiner and pulverize homogenate 1 minute; Obtain homogenate;
4), homogenate is filtered and removed tea grounds by the filter of 200 mesh filter screens, by the filtered fluid obtaining by the high speed centrifugation of 5000rpm, getting after high speed centrifugation the supernatant of gained, to carry out the reverse osmosis concentrated concentration that is reduced to be 150g/L, to be concentrated into concentration be 300g/L to reduction vaporization again, finally spraying is dry, obtains high gamma-aminobutyric acid instant tea.
High gamma-aminobutyric acid instant tea prepared by this law is green powder, detects the alpha-aminobutyric acid content 20.5mg/g of product through HPLC.
Embodiment 3, a kind of method of preparing high gamma-aminobutyric acid instant tea, select the fresh leaves of tea plant (tea tree breed " the ancient tea of water ", bud four leaves 50%, bud five leaves 50%) of bud four, five leaves as tea raw material, to carry out successively following steps:
1), above-mentioned 1000g fresh leaves of tea plant is put into the Stainless steel basin of diameter 35cm, degree of depth 25cm, adding mass concentration is 0.1% glutamic acid aqueous solution 2000g, makes tealeaves evenly drenched;
2), by above-mentioned steps 1) tealeaves that is immersed in glutamic acid aqueous solution of gained is placed in vacuum drying chamber together with rustless steel container, Anaerobic Treatment 8h under-0.08mpa vacuum degree condition; Separate, thus respectively glutamic acid aqueous solution after tealeaves and vacuum Anaerobic Treatment after vacuum Anaerobic Treatment;
3), the tealeaves after vacuum Anaerobic Treatment is put into refiner, add 2000g boiling water to soak for 30 seconds (thereby substituting traditional de-enzyming process);
Again glutamic acid aqueous solution after vacuum Anaerobic Treatment is poured into (object is to recycle) in refiner, opened refiner and pulverize homogenate 3 minutes; Obtain homogenate;
4), homogenate is filtered and removed tea grounds by the filter of 200 mesh filter screens, by the filtered fluid obtaining by the high speed centrifugation of 7000rpm, getting after high speed centrifugation the supernatant of gained, to carry out the reverse osmosis concentrated concentration that is reduced to be 150g/L, to be concentrated into concentration be 350g/L to reduction vaporization again, finally spraying is dry, obtains high gamma-aminobutyric acid instant tea.
High gamma-aminobutyric acid instant tea prepared by this law is green powder, detects the alpha-aminobutyric acid content 25.8mg/g of product through HPLC.
Comparative example 1,, as tea raw material, step is as follows to select the fresh leaves of tea plant (tea tree breed " water ancient tea ") of two leaves and a bud:
1), the fresh leaf of tea tree two leaves and a bud of 1000g is put into the Stainless steel basin of diameter 35cm, degree of depth 20cm, adding mass concentration is 2.5% glutamic acid aqueous solution 1000g, soaks 4h;
2), the above-mentioned tealeaves that is immersed in glutamic acid aqueous solution is pulled out, tealeaves surface moisture is dried up with hair-dryer, be placed in vacuum drying chamber, Anaerobic Treatment 30h under-0.1mpa vacuum degree condition;
3), tealeaves after vacuum Anaerobic Treatment is taken out and puts into refiner, add 2000g boiling water to soak for 10 seconds; Abovementioned steps 1) soak the glutamic acid aqueous solution of fresh leaves of tea plant and pour refiner into and recycle, open refiner tealeaves is pulverized to homogenate 5 minutes;
4), homogenate is filtered and removed tea grounds by the filter of 200 mesh filter screens, by the filtered fluid obtaining by the high speed centrifugation of 7000rpm, getting after high speed centrifugation the supernatant of gained, to carry out the reverse osmosis concentrated concentration that is reduced to be 100g/L, to be concentrated into concentration be 400g/L to reduction vaporization again, last freeze drying, to water content≤5%, obtains high gamma-aminobutyric acid instant tea.
High gamma-aminobutyric acid instant tea prepared by this law is green powder, detects the alpha-aminobutyric acid content 17.6mg/g of product through HPLC.
Comparative example 2, in the step 3) of embodiment 1, do not recycle step 2) produce the aqueous solution, all the other are equal to embodiment 1.Concrete steps are as follows:
1), the fresh leaf of tea tree two leaves and a bud of 1000g (tea tree breed " water ancient tea ") is put into the Stainless steel basin of diameter 35cm, degree of depth 20cm, adding concentration is 2.5% glutamic acid aqueous solution 1000g, makes tealeaves evenly drenched;
2), by above-mentioned steps 1) tealeaves that is immersed in glutamic acid aqueous solution of gained is placed in vacuum drying chamber together with rustless steel container, Anaerobic Treatment 30h under-0.1mpa vacuum degree condition; Separate, thus respectively glutamic acid aqueous solution after tealeaves and vacuum Anaerobic Treatment after vacuum Anaerobic Treatment;
3), the tealeaves after vacuum Anaerobic Treatment is put into refiner, add 2000g boiling water to soak for 10 seconds; Then open refiner tealeaves is pulverized to homogenate 5 minutes;
4), homogenate is filtered and removed tea grounds by the filter of 200 mesh filter screens, by the filtered fluid obtaining by the high speed centrifugation of 7000rpm, getting after high speed centrifugation the supernatant of gained, to carry out the reverse osmosis concentrated concentration that is reduced to be 100g/L, reduction vaporization is concentrated into 400g/L again, last freeze drying, to water content≤5%, obtains high gamma-aminobutyric acid instant tea.
High gamma-aminobutyric acid instant tea prepared by this law is green powder, detects the alpha-aminobutyric acid content 29.6mg/g of product through HPLC.
Comparative example 3,
1), the fresh leaf of tea tree two leaves and a bud of 1000g (tea tree breed " water ancient tea ") is put into the Stainless steel basin of diameter 35cm, degree of depth 20cm, adding mass concentration is 2.5% glutamic acid aqueous solution 1000g, makes tealeaves evenly drenched;
2), by above-mentioned steps 1) tealeaves that is immersed in glutamic acid aqueous solution of gained is placed in vacuum drying chamber together with rustless steel container, Anaerobic Treatment 30h under-0.1mpa vacuum degree condition;
3), the tealeaves after vacuum Anaerobic Treatment is pulled out, dry up with hair-dryer; With 300 ℃ of 8min that complete of roller fixation machine, then knead 15 minutes; Dry 15min, spreading for cooling 2h in dryer at 100-120 ℃; Again at extremely dry tea water content <5% of 80-100 ℃ of oven dry.Dry tea is put into refiner, add 5000ml boiling water to soak also equal oar 40 minutes;
4), homogenate is filtered and removed tea grounds by the filter of 200 mesh filter screens, by the filtered fluid obtaining by the high speed centrifugation of 7000rpm, getting after high speed centrifugation the supernatant of gained, to carry out the reverse osmosis concentrated concentration that is reduced to be 100mg/L, to be concentrated into concentration be 400g/L to reduction vaporization again, last freeze drying, to water content≤5%, obtains high gamma-aminobutyric acid instant tea.
High gamma-aminobutyric acid instant tea prepared by this law is green powder, detects the alpha-aminobutyric acid content 31.1mg/g of product through HPLC.
Finally, it is also to be noted that, what more than enumerate is only several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, can also have many distortion.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention, all should think protection scope of the present invention.
Claims (3)
1. prepare the method for high gamma-aminobutyric acid instant tea, select at least one in the fresh leaves of tea plant of two leaves and a bud～mono-bud five leaves as tea raw material, it is characterized in that comprising following processing step:
(1), fresh leaves of tea plant is immersed in the glutamic acid aqueous solution that the mass concentration of 1～4 times of fresh leaves of tea plant weight is 0.25%～2.5%, thus fresh leaves of tea plant is drenched;
(2), by the tealeaves Anaerobic Treatment 0.5～30h under-0.05～-0.1mpa vacuum degree condition in glutamic acid aqueous solution that is immersed in of above-mentioned steps (1) gained; Separate, thus respectively glutamic acid aqueous solution after tealeaves and vacuum Anaerobic Treatment after vacuum Anaerobic Treatment;
(3), tealeaves after vacuum Anaerobic Treatment is put into refiner, add the boiling water of 1～4 times of step (1) fresh leaves of tea plant weight to soak for 10～30 seconds;
Again glutamic acid aqueous solution after vacuum Anaerobic Treatment is poured in refiner, opened refiner and pulverize homogenate 1-5 minute; Obtain homogenate;
(4), homogenate is filtered and removed tea grounds by the filter of 200 mesh filter screens, by the filtered fluid obtaining by the high speed centrifugation of 5000-7000rpm, get the supernatant of gained after high speed centrifugation and carry out the reverse osmosis concentrated concentration >=100g/L that is reduced to, reduction vaporization is concentrated into concentration >=300g/L again, process to water content≤5% finally by dry, obtain high gamma-aminobutyric acid instant tea.
2. the method for preparing high gamma-aminobutyric acid instant tea according to claim 1, is characterized in that:
In described step (4),
The concentrated technological parameter of reduction vaporization is: temperature is 40～60 ℃, and vacuum is-0.05～-0.1mpa;
Described dry be treated to spray dry or freeze drying.
3. the method for preparing high gamma-aminobutyric acid instant tea according to claim 1 and 2, is characterized in that:
(1), the fresh leaves of tea plant of two leaves and a bud is immersed in the glutamic acid aqueous solution that the mass concentration of 1 times of fresh leaves of tea plant weight is 2.5%, thus fresh leaves of tea plant is fully drenched;
(2), above-mentioned steps (1) gained is immersed in to tealeaves in glutamic acid aqueous solution Anaerobic Treatment 30h under-0.1mpa vacuum degree condition;
(3), tealeaves after vacuum Anaerobic Treatment is put into refiner, add the boiling water of 2 times of steps (1) fresh leaves of tea plant weight to soak for 10 seconds;
Again glutamic acid aqueous solution after vacuum Anaerobic Treatment is poured in refiner, opened refiner and pulverize homogenate 5 minutes; Obtain homogenate;
(4), homogenate is filtered and removed tea grounds by the filter of 200 mesh filter screens, by the filtered fluid obtaining by the high speed centrifugation of 7000rpm, getting after high speed centrifugation the supernatant of gained, to carry out the reverse osmosis concentrated concentration that is reduced to be 100g/L, to be concentrated into concentration be 400g/L to reduction vaporization again, last freeze drying, to water content≤5%, obtains high gamma-aminobutyric acid instant tea powder.
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| CN105614856A (en) * | 2015-12-30 | 2016-06-01 | 福建舜远农业技术开发有限公司 | Preparing process of moringa oleifera nutritive powder rich in selenium and gamma-aminobutyric acid |
| CN105639706A (en) * | 2015-12-30 | 2016-06-08 | 福建舜远农业技术开发有限公司 | Process for manufacturing selenium-rich and gamma-aminobutyric acid-rich Moringa oleifera chewing tablets |
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| CN104687060A (en) * | 2015-02-05 | 2015-06-10 | 云南农业大学 | Moringa oleifera Lam. product rich in gamma-aminobutyric acid (GABA) and processing technology and application thereof |
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| CN105639706A (en) * | 2015-12-30 | 2016-06-08 | 福建舜远农业技术开发有限公司 | Process for manufacturing selenium-rich and gamma-aminobutyric acid-rich Moringa oleifera chewing tablets |
| CN109156567A (en) * | 2018-10-24 | 2019-01-08 | 贵州芳瑞堂生物科技有限公司 | A kind of preparation method of γ-aminobutyric acid green tea |
| US11344042B2 (en) * | 2019-07-08 | 2022-05-31 | May LLC | Method for producing a rosebay willowherb beverage with a high gamma-aminobutyric acid content |
| CN114158635A (en) * | 2021-12-27 | 2022-03-11 | 四川默森药业有限公司 | Mulberry leaf enzyme deactivating method and application thereof in mulberry leaf tea |
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