CN103766531B - Prepare the method for high gamma-aminobutyric acid instant tea - Google Patents
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Abstract
The invention discloses a kind of method preparing high gamma-aminobutyric acid instant tea, mass concentration fresh leaves of tea plant being immersed in 1 ~ 4 times of fresh leaves of tea plant weight is in the glutamic acid aqueous solution of 0.25% ~ 2.5%, Anaerobic Treatment 0.5 ~ 30h under vacuum-0.05 ~-0.1MPa condition, take out tealeaves and put into 1 ~ 4 times of tealeaves weight boiling water soak 10 ~ 30 seconds, merge with the glutamic acid aqueous solution after aforementioned immersion fresh leaves of tea plant, pulverize homogenate 1-5min, 200 mesh filter screens filter, filtrate is through 5000-7000rpm high speed centrifugation, reverse osmosis concentratedly be reduced to concentration >=100g/L, reduction vaporization is concentrated into concentration >=300g/L again, spraying dry or freeze drying are to water content≤5%, obtain the high gamma-aminobutyric acid instant tea of green powder, the alpha-aminobutyric acid content 20.5-35.5mg/g of product.
Description
Technical field
The present invention relates to a kind of preparation method of instant tea powder, be specially the method that one prepares high gamma-aminobutyric acid instant tea (also known as GABA instant tea).
Background technology
GABA (GABA) is a kind of nonprotein amino acid, is present in animal, plant and microorganism.In mammal, GABA is present in brain and spinal cord, it is the inhibitory neurotransmitter of central nervous system, having calm to calm the nerves, reduce blood pressure, treat epilepsy and anticonvulsion, improve brain function, growth promoting effects hormone secretion, promote to sleep, delay senility, strengthen long-term memory and improve the physiologically active such as liver, kidney function, can be applicable to the fields such as medicine, food, beverage, cosmetics.The content of the GABA in ordinary tea leaves is very low, and generally between 0.02 ~ 0.2mg/g, and its content of gamma-aminobutyric acid tea (GABA tea) General Requirements reaches more than 1.5mg/g.
GABA in tealeaves produces through decarboxylic reaction primarily of glutamic acid, and this reaction is by glutamic acid decarboxylase enzymatic.Therefore the glutamic acid in tealeaves is the main producers material of GABA.Also there are some researches show, GABA is relevant with the stress reaction of plant under adverse environmental factor.In anoxic, damage to plants caused by sudden drop in temperature, thermostimulation, mechanical stimulus, arid, under the multiple adverse environmental factor such as salt stress, the alpha-aminobutyric acid content in plant increases.The processing method of GABA tealeaves processing conventional at present has:
1, be sprayed at tea tree with glutamic acid aqueous solution: adopt mass concentration be 0.1% ~ 1.0% glutamic acid aqueous solution be sprayed on fresh leaves of tea plant, fresh leaves of tea plant of gathering after 3-7 days be raw material for processing GABA tea, the GABA content of product is 3.53-4.80mg/g [1].
2,4h is soaked by spreading the sodium glutamate withered to the betel 0.5-3.5% concentration of water content 68%, the remaining sodium glutamate of removing leaf surface is rinsed with clear water, then be placed in vacuum drying chamber Anaerobic Treatment 8h, the GABA content of product is 1.575-2.501mg/g [2].
3, in order to improve vacuum Anaerobic Treatment effect, nitrogen or carbon dioxide is filled with after evacuation.The product G ABA content of process is 1.7647-3.3297mg/g [3].The GABA content of vacuum nitrogen and soaked with liquid process reaches 2.88mg/g and 4.01mg/g [4] respectively.
The glutamic acid solution dipping 5.5h of 4, treatment conditions optimization: draw after being optimized screening to glutamic acid solution concentration, pH and dip time, the condition of optimization is: pH4.8, the GABA content of product reaches 4.5461mg/g [5].
5, GABA instant tea powder processing: by fresh leaves of tea plant at 0.04-0.1pa vacuum, 15-30 DEG C process 2-6h, then by tealeaves successively 100-120 DEG C and 80-100 DEG C of oven dry, gained tealeaves is after the process such as dressing sieve destemming, 15-35min is extracted at 50-80 DEG C, extract through filtering, concentrated and dry, can obtain GABA instant tea powder, the alpha-aminobutyric acid content of product is 10-19mg/g [6].
Existing GABA production of instant tea method is the dry tea of GABA tealeaves prepared especially is raw material, is got, filters, concentrates and spray or freeze drying, make pulverous GABA instant tea powder by water extraction.Do the tea time preparing GABA tealeaves, by glutamic acid or sodium glutamate water-soluble sprinkling tea tree or steeping tea leaves, then fresh leaves of tea plant is filled nitrogen or the process of filling CO 2 condition through vacuum, vacuum person, GABA concentration is impelled to improve, then through kneading, the operation such as dry is processed into the dry tea of GABA tealeaves, can directly brew tea to drink, also can as the raw material of processing GABA instant tea.
The existing method Problems existing producing GABA instant tea for raw material with the dry tea of GABA tealeaves has: 1), need tea-drying when producing the dry tea raw material of GABA tealeaves, again stem tea is immersed in hot water when producing GABA instant tea to extract, energy consumption is large; 2) fresh leaves of tea plant is immersed in glutamic acid aqueous solution process and can improves alpha-aminobutyric acid content, but the GABA having part to generate in this processing procedure has dissolved and has entered the aqueous solution, and these aqueous solution are as discharge of wastewater, both environmental protection was unfavorable for, the materials such as GABA wherein also can not get recycling, the wasting of resources, increases cost.
The bibliography above related to is specific as follows:
1. fixed, Tang Chaqin, Chen Xuan, Xu Deliang, Xiao Runlin, Li Xinghui. Foliar Spraying Amino Acid is on the impact of content of gamma-aminobutyric acid in tea leaves.Tea science, 2006,26 (4): 237-242.
2. mao clear multitude, Dai little Mei, Yang Xinhe, Mu Rubao. vacuum and the process of external source sodium glutamate to gamma-aminobutyric acid in tea leaves enrichment research .Food and Machinery, 2009,25 (4): 49-51.
3. woods intelligence, Lin Zhongming, Yin Junfeng, Tan Junfeng. Anaerobic Treatment is on the impact of content of gamma-aminobutyric acid in tea leaves and quality thereof. Food Science, 2004,25(2): 35-39.
4. yellow sub-brightness, Chen Jianhua, Zeng Zhen, Zheng Hongfa, Huang Huaisheng. improve the technique study of content of gamma-aminobutyric acid in tea leaves. Chinese agronomy circular, 2010,26 (11): 236-240.
5. Liao star. the glutamic acid treatment conditions of tealeaves enrichment GABA optimize .Food and Machinery, 2011,27 (3): 123-126.
6. woods intelligence, Tan Junfeng, Xu Yongquan, Yin Junfeng, Lv Haipeng, Guo Li. a kind of processing method of gamma-aminobutyric acid tea powder. national inventing patent, ZL201110007686.3.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of method preparing high gamma-aminobutyric acid instant tea, the content >=20.5mg/g of GABA in the instant tea that employing the method is prepared and obtained.
The present invention directly to flood through glutamic acid aqueous solution and the fresh leaves of tea plant carrying out vacuum Anaerobic Treatment prepares high gamma-aminobutyric acid instant tea for raw material extracts, reduces the energy consumption of tea-drying process; At leaching process, the aqueous solution after dipping tea fresh leaves is recycled simultaneously, reduce discharge of wastewater, improve the utilization rate of the compositions such as GABA wherein, increase the alpha-aminobutyric acid content of GABA instant tea.The present invention soaks also application of vacuum fresh leaves of tea plant by glutamic acid aqueous solution and promotes generation and the accumulation of gamma-aminobutyric acid in tea leaves, then the fresh tea passes containing GABA after process is soaked in boiling water, pulverizes homogenate, extracts and prepare high gamma-aminobutyric acid instant tea product.
In order to solve the problems of the technologies described above, the invention provides a kind of method preparing high gamma-aminobutyric acid instant tea, select at least one in the fresh leaves of tea plant of two leaves and a bud ~ mono-bud five leaf as tea raw material, carrying out following processing step successively:
(1) mass concentration, by fresh leaves of tea plant being immersed in 1 ~ 4 times of fresh leaves of tea plant weight is in the glutamic acid aqueous solution of 0.25% ~ 2.5%, thus fresh leaves of tea plant is drenched;
Remarks illustrate: make fresh leaves of tea plant by fully drenched as long as this soaks, that is, generally only need soak 3 ~ 5 minutes;
(2), by tealeaves (that is, comprising the fresh leaves of tea plant+glutamic acid aqueous solution after the immersion) Anaerobic Treatment 0.5 ~ 30h under-0.05 ~-0.1mpa vacuum degree condition be immersed in glutamic acid aqueous solution of above-mentioned steps (1) gained; Be separated, thus respectively glutamic acid aqueous solution after tealeaves and vacuum Anaerobic Treatment after vacuum Anaerobic Treatment;
(3), by tealeaves after vacuum Anaerobic Treatment put into refiner, the boiling water adding 1 ~ 4 times of step (1) fresh leaves of tea plant weight soaked for 10 ~ 30 seconds (thus substituting traditional de-enzyming process);
Again glutamic acid aqueous solution after vacuum Anaerobic Treatment is poured into (object recycles) in refiner, open refiner (by tealeaves) and pulverize homogenate 1-5 minute; Obtain homogenate;
(4), homogenate is removed tea grounds by the metre filter of 200 mesh filter screens, by the filtered fluid that the obtains high speed centrifugation by 5000-7000rpm, after getting high speed centrifugation, the supernatant of gained carries out reverse osmosis concentratedly being reduced to concentration >=100g/L, reduction vaporization is concentrated into concentration >=300g/L again, finally by drying process to water content≤5%(% by weight), obtain high gamma-aminobutyric acid instant tea.
Remarks illustrate: the immersion of step (1) can be carried out in rustless steel container.The Anaerobic Treatment of step (2) can be carried out in vacuum drying chamber.
As the improvement preparing the method for high gamma-aminobutyric acid instant tea of the present invention:
In described step (4),
The technological parameter that reduction vaporization concentrates is: temperature is 40 ~ 60 DEG C, and vacuum is-0.05 ~-0.1mpa;
Described drying is treated to spraying dry or freeze drying.
In the present invention,
Rely in step (1) and (2), glutamate molecule can be made to penetrate into tea leaf inside and fully contact with Pidolidone decarboxylase, promote that glutamic acid decarboxylase reaction produces GABA, thus improve the content of gamma-aminobutyric acid in tea leaves.
Rely on the immersion treatment adding boiling water of step (3), traditional tea green-keeping technique can be substituted, the air heat transfer completed than tradition due to heat transfer water is quicker and efficient, and therefore can realize the object completed fast, fixation time shortens to 10-30 second by traditional roller fixation 8-10 minute.
Rely on glutamic acid aqueous solution after vacuum Anaerobic Treatment poured into refiner recycling and opening refiner in step (3) that tealeaves is pulverized homogenate 1-5 minute; Thus realize: utilization pulverizing homogenate substitutes agitator stirring during traditional handicraft extraction tealeaves, tealeaves is extracted more thorough; Extraction time also reduced to 1-5 minute by 30-40 minute of traditional handicraft, saved time.
In step (4), homogenate removes tea grounds by the metre filter of 200 mesh filter screens, filtrate is removed degranulation sediment and other impurity by the high speed centrifugation of 5000-7000rpm, after getting high speed centrifugation, the supernatant of gained carries out reverse osmosis concentratedly being reduced to concentration >=100g/L, be 40 ~ 60 DEG C in temperature again and be concentrated into concentration >=300g/L with vacuum-0.05 ~-0.1mpa reduction vaporization, last spraying dry, intake air temperature 180-190 DEG C, exhaust outlet temperature 80-90 DEG C, obtains the high gamma-aminobutyric acid instant tea powder of water content≤5%; Also by freeze drying to water content≤5%, thus high gamma-aminobutyric acid instant tea powder can be obtained.
Inventive point of the present invention is mainly:
1) conventional instant tea is raw materials used is dry tea, and the present invention is raw materials used is through the fresh leaves of tea plant after vacuum Anaerobic Treatment;
2) the boiling water amount that the present invention is used in preparation process is far smaller than the boiling water amount needed for existing conventional instant tea;
3) conventional instant tea leaching process uses agitator to stir, and tealeaves is carried out pulverizing homogenate by the present invention, makes the extraction of tealeaves more abundant.
The method preparing high gamma-aminobutyric acid instant tea of the present invention, fresh leaves of tea plant is immersed in glutamic acid water-soluble in carry out vacuum Anaerobic Treatment (vacuumizing reduced pressure treatment), the space segment making glutamic acid aqueous solution enter tea leaf inside replaces air wherein, more glutamate molecule is allowed to contact with the Pidolidone decarboxylase of blade interior, promote that glutamic acid decarboxylase reaction produces GABA, improve the content of gamma-aminobutyric acid in tea leaves.
The method preparing high gamma-aminobutyric acid instant tea of the present invention, directly the fresh leaves of tea plant of vacuum decompression process is put into boiling water to soak, substitute traditional tea green-keeping technique, the air heat transfer that speed and efficiency because of heat transfer water complete higher than tradition, can realize completing fast.Step 2 after completing) produce containing GABA the aqueous solution (namely, glutamic acid aqueous solution after vacuum Anaerobic Treatment) pour refiner into and contact with tealeaves with hot water wherein, reduce water temperature, increase the water yield, then tealeaves homogenate is pulverized, reach the object of resource high-efficiency extraction and application.This process achieve simultaneously traditional handicraft tea green-keeping, knead, drying, the operation such as extraction target, have energy consumption low, reduce discharge of wastewater, resource utilization advantages of higher.Meanwhile, because reducing the destruction to chlorophyll and catechin compounds of tea-drying process, the color and luster of product is greener, and exterior quality significantly improves.The alpha-aminobutyric acid content of high gamma-aminobutyric acid instant tea product prepared by the present invention is up to 20.5-35.5mg/g.
In sum, fresh leaves of tea plant is immersed in glutamic acid solution by the present invention, under-0.05 ~-0.1mpa vacuum degree condition, process tealeaves 0.5-30 hour, improves the GABA level of tealeaves; Tealeaves soak through superheated water (boiling water) again complete, pulverize that homogenate is extracted, filtered, the step such as concentrated, spraying dry or freeze drying makes high gamma-aminobutyric acid instant tea powder.The present invention impels glutamic acid aqueous solution to enter tea leaf inside, allows more glutamate molecule contact with the glutamate decarboxylase of blade interior, promotes that glutamic acid decarboxylase reaction produces more GABA; The present invention is completed by hot-water soak, recycling step 2) process tea fresh leaves after the aqueous solution, pulverize homogenate extract, achieve the tea green-keeping of traditional handicraft simultaneously, knead, drying, the operation such as extraction function, have that energy consumption is low, discharge of wastewater is few, resource utilization advantages of higher; The alpha-aminobutyric acid content of product is apparently higher than conventional art.
Detailed description of the invention
Embodiment 1, a kind of method preparing high gamma-aminobutyric acid instant tea, select the fresh leaves of tea plant of two leaves and a bud (tea tree breed " the ancient tea of water ") as tea raw material, to carry out following processing step successively:
1), the fresh leaves of tea plant of 1000g two leaves and a bud is put into the Stainless steel basin of diameter 35cm, degree of depth 20cm, add the glutamic acid aqueous solution 1000g that mass concentration is 2.5%, tealeaves is evenly drenched;
2), by above-mentioned steps 1) tealeaves (that is, comprising the fresh leaves of tea plant+glutamic acid aqueous solution after immersion) be immersed in glutamic acid aqueous solution of gained is placed in vacuum drying chamber together with rustless steel container, Anaerobic Treatment 30h under-0.1mpa vacuum degree condition; Be separated, thus respectively glutamic acid aqueous solution after tealeaves and vacuum Anaerobic Treatment after vacuum Anaerobic Treatment;
3), by tealeaves after vacuum Anaerobic Treatment put into refiner, add 2000g boiling water and soaked for 10 seconds (thus substituting traditional de-enzyming process);
Again glutamic acid aqueous solution after vacuum Anaerobic Treatment is poured into (object recycles) in refiner, open refiner and tealeaves is pulverized homogenate 5 minutes; Obtain homogenate;
4), homogenate is removed tea grounds by the metre filter of 200 mesh filter screens, by the filtered fluid that the obtains high speed centrifugation by 7000rpm, after getting high speed centrifugation, the supernatant of gained carries out the reverse osmosis concentrated concentration that is reduced to is 100g/L, concentrated (temperature is 40 ~ 60 DEG C to reduction vaporization again, vacuum is-0.05 ~-0.1mpa) to concentration be 400g/L, last freeze drying, to water content≤5%, namely obtains high gamma-aminobutyric acid instant tea powder.
High gamma-aminobutyric acid instant tea prepared by this law is green powder, detects, the alpha-aminobutyric acid content 35.5mg/g of product through HPLC.
Embodiment 2, a kind of method preparing high gamma-aminobutyric acid instant tea, select fresh leaves of tea plant (tea tree breed " Fuding white tea ", two leaves and a bud 50%, bud three leaf 50%) as tea raw material, to carry out following processing step successively:
1), above-mentioned for 1000g fresh leaves of tea plant is put into the Stainless steel basin of diameter 45cm, degree of depth 30cm, add the glutamic acid aqueous solution 4000g that mass concentration is 0.25%, tealeaves is evenly drenched;
2), by above-mentioned steps 1) tealeaves being immersed in glutamic acid aqueous solution of gained is placed in vacuum drying chamber together with rustless steel container, Anaerobic Treatment 0.5h under-0.05ma vacuum degree condition; Be separated, thus respectively glutamic acid aqueous solution after tealeaves and vacuum Anaerobic Treatment after vacuum Anaerobic Treatment;
3), by the tealeaves after vacuum Anaerobic Treatment put into refiner, add 1000g boiling water and soaked for 30 seconds (thus substituting traditional de-enzyming process);
Again glutamic acid aqueous solution after vacuum Anaerobic Treatment is poured into (object recycles) in refiner, open refiner and pulverize homogenate 1 minute; Obtain homogenate;
4), homogenate is removed tea grounds by the metre filter of 200 mesh filter screens, by the filtered fluid that the obtains high speed centrifugation by 5000rpm, after getting high speed centrifugation, the supernatant of gained carries out the reverse osmosis concentrated concentration that is reduced to is 150g/L, reduction vaporization is concentrated into concentration is again 300g/L, last spraying dry, namely obtains high gamma-aminobutyric acid instant tea.
High gamma-aminobutyric acid instant tea prepared by this law is green powder, detects, the alpha-aminobutyric acid content 20.5mg/g of product through HPLC.
Embodiment 3, a kind of method preparing high gamma-aminobutyric acid instant tea, select the fresh leaves of tea plant of bud four, five leaf (tea tree breed " the ancient tea of water ", bud four leaf 50%, bud five leaf 50%) as tea raw material, to carry out following steps successively:
1), above-mentioned for 1000g fresh leaves of tea plant is put into the Stainless steel basin of diameter 35cm, degree of depth 25cm, add the glutamic acid aqueous solution 2000g that mass concentration is 0.1%, tealeaves is evenly drenched;
2), by above-mentioned steps 1) tealeaves being immersed in glutamic acid aqueous solution of gained is placed in vacuum drying chamber together with rustless steel container, Anaerobic Treatment 8h under-0.08mpa vacuum degree condition; Be separated, thus respectively glutamic acid aqueous solution after tealeaves and vacuum Anaerobic Treatment after vacuum Anaerobic Treatment;
3), by the tealeaves after vacuum Anaerobic Treatment put into refiner, add 2000g boiling water and soaked for 30 seconds (thus substituting traditional de-enzyming process);
Again glutamic acid aqueous solution after vacuum Anaerobic Treatment is poured into (object recycles) in refiner, open refiner and pulverize homogenate 3 minutes; Obtain homogenate;
4), homogenate is removed tea grounds by the metre filter of 200 mesh filter screens, by the filtered fluid that the obtains high speed centrifugation by 7000rpm, after getting high speed centrifugation, the supernatant of gained carries out the reverse osmosis concentrated concentration that is reduced to is 150g/L, reduction vaporization is concentrated into concentration is again 350g/L, last spraying dry, namely obtains high gamma-aminobutyric acid instant tea.
High gamma-aminobutyric acid instant tea prepared by this law is green powder, detects, the alpha-aminobutyric acid content 25.8mg/g of product through HPLC.
Comparative example 1, as tea raw material, step is as follows to select the fresh leaves of tea plant (tea tree breed " water ancient tea ") of two leaves and a bud:
1), the fresh leaf of tea tree two leaves and a bud of 1000g is put into the Stainless steel basin of diameter 35cm, degree of depth 20cm, add the glutamic acid aqueous solution 1000g that mass concentration is 2.5%, soak 4h;
2), by the above-mentioned tealeaves being immersed in glutamic acid aqueous solution pull out, with hair-dryer, tealeaves surface moisture is dried up, be placed in vacuum drying chamber, Anaerobic Treatment 30h under-0.1mpa vacuum degree condition;
3), by the tealeaves after vacuum Anaerobic Treatment take out and put into refiner, add 2000g boiling water and soaked for 10 seconds; Abovementioned steps 1) soak the glutamic acid aqueous solution of fresh leaves of tea plant and pour refiner into and recycle, open refiner and tealeaves is pulverized homogenate 5 minutes;
4), homogenate is removed tea grounds by the metre filter of 200 mesh filter screens, by the filtered fluid that the obtains high speed centrifugation by 7000rpm, after getting high speed centrifugation, the supernatant of gained carries out the reverse osmosis concentrated concentration that is reduced to is 100g/L, reduction vaporization is concentrated into concentration is again 400g/L, last freeze drying, to water content≤5%, namely obtains high gamma-aminobutyric acid instant tea.
High gamma-aminobutyric acid instant tea prepared by this law is green powder, detects, the alpha-aminobutyric acid content 17.6mg/g of product through HPLC.
Comparative example 2, in the step 3) of embodiment 1, do not recycle step 2) aqueous solution that produces, all the other are equal to embodiment 1.Concrete steps are as follows:
1), the fresh leaf of tea tree two leaves and a bud of 1000g (tea tree breed " the ancient tea of water ") is put into the Stainless steel basin of diameter 35cm, degree of depth 20cm, add the glutamic acid aqueous solution 1000g that concentration is 2.5%, tealeaves is evenly drenched;
2), by above-mentioned steps 1) tealeaves being immersed in glutamic acid aqueous solution of gained is placed in vacuum drying chamber together with rustless steel container, Anaerobic Treatment 30h under-0.1mpa vacuum degree condition; Be separated, thus respectively glutamic acid aqueous solution after tealeaves and vacuum Anaerobic Treatment after vacuum Anaerobic Treatment;
3), by the tealeaves after vacuum Anaerobic Treatment put into refiner, add 2000g boiling water and soaked for 10 seconds; Then open refiner and tealeaves is pulverized homogenate 5 minutes;
4), homogenate is removed tea grounds by the metre filter of 200 mesh filter screens, by the filtered fluid that the obtains high speed centrifugation by 7000rpm, after getting high speed centrifugation, the supernatant of gained carries out the reverse osmosis concentrated concentration that is reduced to is 100g/L, reduction vaporization is concentrated into 400g/L again, last freeze drying, to water content≤5%, namely obtains high gamma-aminobutyric acid instant tea.
High gamma-aminobutyric acid instant tea prepared by this law is green powder, detects, the alpha-aminobutyric acid content 29.6mg/g of product through HPLC.
Comparative example 3,
1), the fresh leaf of tea tree two leaves and a bud of 1000g (tea tree breed " the ancient tea of water ") is put into the Stainless steel basin of diameter 35cm, degree of depth 20cm, add the glutamic acid aqueous solution 1000g that mass concentration is 2.5%, tealeaves is evenly drenched;
2), by above-mentioned steps 1) tealeaves being immersed in glutamic acid aqueous solution of gained is placed in vacuum drying chamber together with rustless steel container, Anaerobic Treatment 30h under-0.1mpa vacuum degree condition;
3), by the tealeaves after vacuum Anaerobic Treatment pull out, dry up with hair-dryer; With roller fixation machine 300 DEG C of 8min that complete, then knead 15 minutes; 15min, spreading for cooling 2h is dried at 100-120 DEG C in dryer; Dry to dry tea water content <5% at 80-100 DEG C again.Dry tea is put into refiner, adds 5000ml boiling water and soak and equal oars 40 minutes;
4), homogenate is removed tea grounds by the metre filter of 200 mesh filter screens, by the filtered fluid that the obtains high speed centrifugation by 7000rpm, after getting high speed centrifugation, the supernatant of gained carries out the reverse osmosis concentrated concentration that is reduced to is 100mg/L, reduction vaporization is concentrated into concentration is again 400g/L, last freeze drying, to water content≤5%, namely obtains high gamma-aminobutyric acid instant tea.
High gamma-aminobutyric acid instant tea prepared by this law is green powder, detects, the alpha-aminobutyric acid content 31.1mg/g of product through HPLC.
Finally, it is also to be noted that what enumerate above is only several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be had.All distortion that those of ordinary skill in the art can directly derive from content disclosed by the invention or associate, all should think protection scope of the present invention.
Claims (3)
1. prepare the method for high gamma-aminobutyric acid instant tea, select at least one in the fresh leaves of tea plant of two leaves and a bud ~ mono-bud five leaf as tea raw material, it is characterized in that comprising following processing step:
(1) mass concentration, by fresh leaves of tea plant being immersed in 1 ~ 4 times of fresh leaves of tea plant weight is in the glutamic acid aqueous solution of 0.25% ~ 2.5%, thus fresh leaves of tea plant is drenched; Soak time is 3 ~ 5 minutes;
(2), by the tealeaves Anaerobic Treatment 0.5 ~ 30h under-0.05 ~-0.1Mpa vacuum degree condition be immersed in glutamic acid aqueous solution of above-mentioned steps (1) gained; Be separated, thus respectively glutamic acid aqueous solution after tealeaves and vacuum Anaerobic Treatment after vacuum Anaerobic Treatment;
(3), by tealeaves after vacuum Anaerobic Treatment put into refiner, the boiling water adding 1 ~ 4 times of step (1) fresh leaves of tea plant weight soaked for 10 ~ 30 seconds;
Again glutamic acid aqueous solution after vacuum Anaerobic Treatment is poured in refiner, open refiner and pulverize homogenate 1-5 minute; Obtain homogenate;
(4), homogenate is removed tea grounds by the metre filter of 200 mesh filter screens, by the filtered fluid that the obtains high speed centrifugation by 5000-7000rpm, after getting high speed centrifugation, the supernatant of gained carries out reverse osmosis concentratedly being reduced to concentration >=100g/L, reduction vaporization is concentrated into concentration >=300g/L again, finally by drying process to water content≤5%, obtain high gamma-aminobutyric acid instant tea.
2. the method preparing high gamma-aminobutyric acid instant tea according to claim 1, is characterized in that:
In described step (4),
The technological parameter that reduction vaporization concentrates is: temperature is 40 ~ 60 DEG C, and vacuum is-0.05 ~-0.1Mpa;
Described drying is treated to spraying dry or freeze drying.
3. the method preparing high gamma-aminobutyric acid instant tea according to claim 1 and 2, is characterized in that:
(1) mass concentration, by the fresh leaves of tea plant of two leaves and a bud being immersed in 1 times of fresh leaves of tea plant weight is in the glutamic acid aqueous solution of 2.5%, thus fresh leaves of tea plant is fully drenched;
(2), by the tealeaves Anaerobic Treatment 30h under-0.1Mpa vacuum degree condition be immersed in glutamic acid aqueous solution of above-mentioned steps (1) gained;
(3), by tealeaves after vacuum Anaerobic Treatment put into refiner, the boiling water adding 2 times of step (1) fresh leaves of tea plant weight soaked for 10 seconds;
Again glutamic acid aqueous solution after vacuum Anaerobic Treatment is poured in refiner, open refiner and pulverize homogenate 5 minutes; Obtain homogenate;
4), homogenate is removed tea grounds by the metre filter of 200 mesh filter screens, by the filtered fluid that the obtains high speed centrifugation by 7000rpm, after getting high speed centrifugation, the supernatant of gained carries out the reverse osmosis concentrated concentration that is reduced to is 100g/L, reduction vaporization is concentrated into concentration is again 400g/L, last freeze drying, to water content≤5%, obtains high gamma-aminobutyric acid instant tea powder.
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| CN114158635A (en) * | 2021-12-27 | 2022-03-11 | 四川默森药业有限公司 | Mulberry leaf enzyme deactivating method and application thereof in mulberry leaf tea |
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