CN103756873B - Photobioreactor for cultivating haematococcus pluvialis - Google Patents
Photobioreactor for cultivating haematococcus pluvialis Download PDFInfo
- Publication number
- CN103756873B CN103756873B CN201410022508.1A CN201410022508A CN103756873B CN 103756873 B CN103756873 B CN 103756873B CN 201410022508 A CN201410022508 A CN 201410022508A CN 103756873 B CN103756873 B CN 103756873B
- Authority
- CN
- China
- Prior art keywords
- pipeline
- culture
- tubes
- haematococcus pluvialis
- column
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/10—Rotating vessel
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a photobioreactor for cultivating haematococcus pluvialis. The photobioreactor comprises an upright stand, a base, culture tubes, a top cover, a spherical rotor, a rubber plug and a motor, wherein two layers of culture tubes are arranged into an umbrella shape; the tubes at the upper layer are used for transformation culture of haematococcus pluvialis and directly exposed to light, so that the culture solution is quickly heated under strong light irradiation and advantageous for efficient transformation; the tubes of the lower layer are used for nutrient culture haematococcus pluvialis, and under condition that light is shielded by the tubes at the upper layer, temperature rise in the tubes is not obvious, which is advantageous for nutritional growth of the haematococcus pluvialis; the umbrella shape design, in combination with the environmental conditions required by nutritional growth and transformation, is capable of realizing efficient culture; a rotating shaft is arranged at the middle part of the upright stand and connected with the spherical rotor in the base, the spherical rotor is connected with the motor, and the motor runs to drive the umbrella-shaped photobioreactor to rotate; as a result, the surface of the culture solution is in sufficient contact with air, which is advantageous for photosynthesis, and furthermore, the destruction of the haematococcus pluvialis caused by shear force generated by air inflation is avoided.
Description
Technical field
The present invention relates to the culture device of Haematocoocus Pluvialls, be specifically related to a kind of bioreactor cultivating Haematocoocus Pluvialls.
Background technology
Haematocoocus Pluvialls (Haematococcus pluvialis) is a kind of unicell green alga producing natural astaxanthin, and its astaxanthin substances content is high, is regarded as " concentrate " of natural astaxanthin.Astaxanthin is the material that occurring in nature resistance of oxidation is the strongest, is ascorbic 20 times, has alleviating fatigue, delays senility and the effect of prevention and therapy cardiovascular and cerebrovascular diseases; Also be widely used on the feed of salmon, the salmon of cultivation has good mouthfeel and nutritive value.Due to stability and the security of natural astaxanthin structure, at present, cultivating Haematocoocus Pluvialls is the new bio technical industry obtaining astaxanthin, is subject to carrying forward vigorously of various countries.
Pilot scale culture Haematocoocus Pluvialls mainly contains two kinds of modes: one is the bioreactor of closed cultivation, adopt and be layered on ground, have certain obliquity or upright transparent pipeline as the sealing-duct system of cultivating, the nutrient solution of flowing is set in pipeline, under the conditions such as control temperature, light intensity, substratum, cultivates Haematocoocus Pluvialls; This kind of training method makes each stage of cultivation reasonably reach optimal culture condition, can realize the Rapid Accumulation of high-density culture and product, can also avoid the harm of extraneous harmful organisms, reduces production cost, realizes semicontinuous even cultured continuously; But, but investment is large, and how adding nutritive salt in time, getting rid of too much oxygen is also the technical barrier faced.Two is open culture systems, comprises cultivation pool, conduit and whipping appts; The scale of this kind of training method is easily amplified, high financial profit, at present as the main training method of Haematocoocus Pluvialls; But this kind of training method culturing process is easily affected by the external environment, and be comparatively hard to keep the suitableeest temperature and illumination, cannot ensure the highest speed of growth and cell density, recovery process and equipment also need to update.
Employing Haematocoocus Pluvialls cultivation link has two crucial stages, and one is the process of nourishing and growing, and need normal temperature, the low light level, high nutritive salt, carbon/nitrogen is than little; Two is transform and accumulation, needs high temperature, high light, high nutritive salt, and carbon/nitrogen is than large.How designing and Appropriate application production unit, improve the element of resources such as light, temperature, soil, is the key realizing high effect culture Haematocoocus Pluvialls and produce astaxanthin.At present, bioreactor technology is a kind of technology of novel cultivation patter of rain haematococcus pulvialis, a kind of production model taking single stage method of bioreactor studio, namely in same bio-reactor, needed the cultivation of Vegetative Cell of Haematococcus Pluvialis to increase, transformed and Accumulation of Astaxanthin (if notification number is CN1966660, CN102134553A, CN1680539, CN102864070A, CN102559477A, CN1966660).But, also there is no the special bioreactor based on continental rise for haematococcus pluvialis to produce astaxanthin at present.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of umbellate form bioreactor cultivating Haematocoocus Pluvialls, and different for the condition of nourishing and growing from required by conversion, design is specifically designed to the umbellate form bioreactor that Haematocoocus Pluvialls is cultivated.
The present invention realizes like this, a kind of umbellate form light thing reactor cultivating Haematocoocus Pluvialls, comprise the pedestal that a vertical passive column and play fixed action, it is characterized in that, described column inside has a rotating shaft, described base interior has a ball-type rotor, and the bottom of rotating shaft is connected by contacting with spherical spinner; Column external stability connects the pipeline in umbellate form.
Described bioreactor also comprises a motor, is connected with ball-type rotor.
Above-mentioned pipeline has two-layer up and down, and upper strata pipeline is umbellate form, and lower floor's pipeline is in being inverted umbellate form.
The above-mentioned one end open of upper strata pipeline near column top, pipeline opening inner edge is fixedly connected on column, has top cover above the port of pipeline; The other end of upper strata pipeline is opening-like, and the inner edge of pipeline opening is fixedly connected with the pipeline opening inner edge of lower floor's pipeline, and pipeline opening has soft rubber ball.
Above-mentioned lower floor's pipeline is closed near one end of column bottom, and pipeline opening inner edge is fixedly connected on column; The other end of lower floor's pipeline is opening-like, and pipeline opening inner edge is fixedly connected with the pipeline opening inner edge of upper strata pipeline, and pipeline opening has soft rubber ball.
Umbellate form bioreactor of the present invention has upper and lower two-layer cultivation pipeline, the layout of pipeline is umbellate form, upper strata pipeline is used for Haematocoocus Pluvialls and transforms cultivation, under pipeline is directly exposed to illumination condition, high light can be obtained, under strong illumination, nutrient solution rapid temperature increases in this pipeline, is conducive to Efficient Conversion; Lower floor's pipeline is used for Haematocoocus Pluvialls nutrition and cultivates, and under the dark conditions of upper strata pipeline, lower floor's pipeline can obtain relatively low illumination, and the intensification in pipeline is not obvious, is conducive to nourishing and growing of Haematocoocus Pluvialls; Umbellate form design in conjunction with the required envrionment conditions with conversion of nourishing and growing, can realize high-efficient culture.Be rotating shaft in the middle of column, be connected with pedestal, and connecting motor, motor running drives umbellate form bioreactor to rotate, nutrient solution surface fully can contact with air, is conducive to photosynthesis, and avoids the destructive force of shearing force for Haematocoocus Pluvialls of inflation generation.
Accompanying drawing explanation
Fig. 1: one-piece construction schematic diagram of the present invention;
Fig. 2: one-piece construction diagrammatic cross-section of the present invention;
Fig. 3: one-piece construction vertical view of the present invention;
Wherein: 1, column 2, pipeline 21, upper strata pipeline 22, lower floor's pipeline 3, top cover 4, pedestal 5, ball-type rotor 6, rotating shaft 7, soft rubber ball.
Embodiment
Below in conjunction with embodiment and accompanying drawing, the umbellate form light thing reactor that the present invention cultivates Haematocoocus Pluvialls is further described in detail.
As shown in Figure 1, Figure 2 and Figure 3, cultivate the umbellate form light thing reactor of Haematocoocus Pluvialls, comprise the pedestal 4 that a vertical passive column 1 and play fixed action, the inside of column 1 has a rotating shaft 6, the inside of pedestal 4 has a ball-type rotor 5, and the bottom of rotating shaft 6 connects by contacting with ball-type rotor 5.Ball-type rotor 5 also with a motors, drive ball-type rotor 5 fast rotational after motor starting, the rotating shaft contacted with ball-type rotor 5 is rotated thereupon, thus whole culture apparatus can be driven to rotate together.Column 1 external stability connected double-layer pipeline 2, upper strata pipeline 21 is in umbellate form, and lower floor's pipeline 22 is in being inverted umbellate form.
Upper strata pipeline 21 is near the one end open on column 1 top, the algae kind of nutrient solution and Haematocoocus Pluvialls can be added by this opening end, pipeline opening inner edge is fixedly connected on column, has top cover 3 above the port of pipeline, and after adding nutrient solution and bacterial classification, top cover 3 can avoid strain pollution; The other end of upper strata pipeline 21 is opening-like, the inner edge of pipeline opening is fixedly connected with the pipeline opening inner edge of lower floor pipeline 22, pipeline opening has soft rubber ball, can prevent nutrient solution and bacterial classification from exposing after clogging soft rubber ball, opens soft rubber ball and namely can collect cultured Haematocoocus Pluvialls.
Lower floor's pipeline 22 is closed near one end of column 1 bottom, and pipeline opening inner edge is fixedly connected on column 1; The other end of lower floor's pipeline 22 is opening-like, and pipeline opening inner edge is fixedly connected with the pipeline opening inner edge of upper strata pipeline 21, and pipeline opening has soft rubber ball; Open soft rubber ball and namely can add nutrient solution and bacterial classification, or collect product by siphoning installation.
When the present invention uses, first carry out Haematocoocus Pluvialls inoculation after equipment washing is clean, inoculation time is preferably the morning of every day; Culture medium prescription is preferably the KNO of 300g
3, 20g K
2hPO
4, 2.5g FeSO
4, 10g the VB of EDTA, 6mg
1with the VB of 50ug
12heating is dissolved in 1000ml fresh water and is made into mother liquor, and every 1000ml nutrient solution adds 1ml mother liquor.Control the condition of cultivating in culturing process, temperature is preferably 20-28 DEG C, and lower floor's light intensity is preferably 500-800LX, and light source is natural light; Shake three every day, when shaking, nutrient solution is shaken out, rotating speed is 20 turns/min.
After cultivation, haematococcus pluvialis cell volumetric production is up to 0.1-0.7g/L, natural astaxanthin content can up to the 1.2-2.8% of dry cell weight, than ordinary method do sth. in advance 12-36 little time, obtain through being separated operation such as results frustule and cryodrying etc. the frustule being rich in astaxanthin.Method of the present invention is simple, with low cost, and land utilization ratio is high, and shortens the transport that Haematocoocus Pluvialls cultivating from nourishing and growing to transforming, thus improves the efficiency of haematococcus pluvialis to produce astaxanthin, has good industrialization prospect.
Claims (2)
1. cultivate the umbellate form bioreactor of Haematocoocus Pluvialls for one kind, comprise the pedestal (4) that a vertical passive column (1) and play fixed action, it is characterized in that, described column (1) inside has a rotating shaft (6), described pedestal (4) inside has a ball-type rotor (5), and the bottom of rotating shaft is connected by contacting with ball-type rotor (5); Column (1) external stability connects the pipeline (2) in umbellate form; Described pipeline (2) has two-layer up and down, and upper strata pipeline (21) is in umbellate form, and lower floor's pipeline (22) is in being inverted umbellate form; Described upper strata pipeline (21) is near the one end open on column (1) top, and pipeline opening inner edge is fixedly connected on column (1), has top cover (3) above the port of pipeline; The other end of upper strata pipeline (21) is opening-like, and the inner edge of pipeline opening is fixedly connected with the pipeline opening inner edge of lower floor's pipeline (22), and pipeline opening has soft rubber ball (7); Described lower floor's pipeline (22) is closed near one end of column bottom, and pipeline opening inner edge is fixedly connected on column; The other end of lower floor's pipeline (22) is opening-like, and pipeline opening inner edge is fixedly connected with the pipeline opening inner edge of upper strata pipeline, and pipeline opening has soft rubber ball (7).
2. umbellate form bioreactor according to claim 1, is characterized in that, described bioreactor also comprises a motor, is connected with ball-type rotor (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410022508.1A CN103756873B (en) | 2014-01-17 | 2014-01-17 | Photobioreactor for cultivating haematococcus pluvialis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410022508.1A CN103756873B (en) | 2014-01-17 | 2014-01-17 | Photobioreactor for cultivating haematococcus pluvialis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103756873A CN103756873A (en) | 2014-04-30 |
| CN103756873B true CN103756873B (en) | 2015-04-15 |
Family
ID=50524201
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410022508.1A Expired - Fee Related CN103756873B (en) | 2014-01-17 | 2014-01-17 | Photobioreactor for cultivating haematococcus pluvialis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103756873B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110423675A (en) * | 2019-09-11 | 2019-11-08 | 宁波大学 | A kind of haematococcus pluvialis large-scale farming device made using ecological niche principle |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101406895B (en) * | 2008-12-05 | 2010-06-30 | 陕西紫兆环保产业开发有限责任公司 | Hydrolytic reaction pot for realtime processing mixed consumer waste |
| JP2010148404A (en) * | 2008-12-24 | 2010-07-08 | Hidenori Shimoji | Method for culturing marine alga with three-dimensional net |
| CN102320690B (en) * | 2011-08-29 | 2013-04-10 | 华东师范大学 | Method for controllably removing water-body eutrophication by utilizing fast-growing aquatic plant |
| CN202285667U (en) * | 2011-10-09 | 2012-07-04 | 南通天华和睿科技创业有限公司 | Umbrella type rotary stereoscopic cultivation device |
-
2014
- 2014-01-17 CN CN201410022508.1A patent/CN103756873B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN103756873A (en) | 2014-04-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Yen et al. | Design of photobioreactors for algal cultivation | |
| Borowitzka et al. | Scaling up microalgal cultures to commercial scale | |
| Borowitzka | Energy from microalgae: a short history | |
| Grivalský et al. | Development of thin-layer cascades for microalgae cultivation: milestones | |
| Xu et al. | Microalgal bioreactors: challenges and opportunities | |
| Barghbani et al. | Investigating the effects of several parameters on the growth of Chlorella vulgaris using Taguchi's experimental approach | |
| CN103451091B (en) | Device for purifying livestock and poultry breeding wastewater by microalgae and method thereof | |
| CN106133147A (en) | The production method of astaxanthin | |
| CN207070719U (en) | A kind of equipment grown soon suitable for aquaculture seedling | |
| CN105165592A (en) | Large-scale algae cultivation device | |
| EP3902389A1 (en) | A microalgae-based system for producing products and a process using thereof | |
| CN103756873B (en) | Photobioreactor for cultivating haematococcus pluvialis | |
| CN104726321B (en) | A kind of racetrack bioreactor suitable for sunlight batch production | |
| CN109679853A (en) | The method for improving haematococcus pluvialis biomass and astaxanthin yield using fulvic acid | |
| CN205993192U (en) | The rotatable multilayer vegetable plantation device of family room's inner circular | |
| CN107098478A (en) | A kind of microalgae carbon sequestration and sewage ecological treatment system | |
| CN206674721U (en) | A kind of portable culture apparatus of desert algae kind | |
| CN205320953U (en) | Cigarette aphidiidae cultivates system | |
| CN106754385A (en) | A kind of utilization blue-green alga bloom is the method for raw material cultivation Chlorella phytoplankton | |
| CN209260101U (en) | A kind of both culturing microalgae system in ocean deepwater area | |
| CN108285859B (en) | Novel pneumatic nostoc algae runway pond culture system | |
| CN106508492A (en) | Crystal-containing pyramid-type factory farming automatic production line | |
| CN206879704U (en) | A kind of cray cultivates dedicated multi-layered secondary pasture and water planting unit | |
| TW201806477A (en) | Artificial cultivation method of wild Antrodia cinnamomea and equipment thereof characterized by simulating the wild environment of Antrodia cinnamomea to artificially cultivate wild Antrodia cinnamomea | |
| CN205284355U (en) | Equipment is cultivateed with plant in gardens |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20171026 Address after: 674202 Lijiang City, Yunnan province Yongsheng County Cheng Hai Zhen East Lake Cun Wei Hui Guan Village No. 18 Patentee after: Tan Yinghong Address before: 315211 Zhejiang Province, Ningbo Jiangbei District Fenghua Road No. 818 Co-patentee before: Lijiang Chenghai Bao'er Biological Development Co., Ltd. Patentee before: Ningbo University |
|
| TR01 | Transfer of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150415 Termination date: 20180117 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |