CN103740595A - Method for producing chlorella concentrated solution - Google Patents
Method for producing chlorella concentrated solution Download PDFInfo
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- CN103740595A CN103740595A CN201410031621.6A CN201410031621A CN103740595A CN 103740595 A CN103740595 A CN 103740595A CN 201410031621 A CN201410031621 A CN 201410031621A CN 103740595 A CN103740595 A CN 103740595A
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- chlorella
- concentrated solution
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- ultrafiltration
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Abstract
The invention discloses a method for producing a chlorella concentrated solution, which belongs to the field of concentration and purification of living microalgae. The preparation method mainly comprises filtration and microfiltration/ultrafiltration. The method is particularly suitable for preparing the chlorella concentrated solution, and the prepared chlorella concentrated solution is high in purity, high in concentration efficiency, free of flocculant residue, stable in quality, high in chlorella content and long in storage time.
Description
Technical field
The present invention relates to a kind of production method of chlorella concentrated solution, particularly relate to a kind of based on filtering, the production method of concentrated chlorella concentrated solution, belong to the concentrated and purified field of micro-algae.
Background technology
Chlorella (Chlorella) is the general natural disposition unicell green alga of Chlorophyta Chlorella, it is a kind of spherical unicellular fresh water algae, 3～8 microns of diameters, one of life the earliest on the earth, appeared at before more than 20 hundred million years, gene does not change all the time, is a kind of efficient photosynthetic plant, with photoautotrophy growth and breeding, distributed pole is wide.Common chlorella has Chlorella pyrenoidesa, the raw chlorella of salt, eyespot chlorella, avette chlorella and sea growing chlorella.To breed like spore, it is the only reproduction type of kind in Chlorella.
Chlorella contains rich in protein, VITAMIN, mineral substance, foodstuff fibre, nucleic acid and chlorophyll etc., be the indispensable nutrient substance that maintains and promote health, particularly contain compelling biologically active substance glycoprotein, polysaccharide body and up to 13% materials such as nucleic acid.Have and strengthen human immunity, prevent virus multiplication, anticancer propagation, suppress blood sugar and rise, reduce serum cholesterol content, get rid of toxin, the functions such as damage of repairing rapidly body.
Because chlorella contains abundant nutritive substance, therefore of many uses, be often used in following aspect:
1) nutritional additive--protein, chlorophyll, VITAMIN, mineral substance, the nucleic acid of high-content are provided;
2) as food dye extract;
3) feed-fishes and shrimps open-mouthed bait;
4) cosmetic material.
Along with updating of cultural technique, under traditional aquaculture model, pond dosage is large, the specification of breed variety is little and have a drug residue, by the application of chlorella, can well improve cultural method, reduce the use of medicine, form a kind of green ecological aquaculture model, reduce aquaculture cost, improve output, increase raiser's income.
The Main Function of chlorella in cultivation:
1) provide bait effect
Chlorella contains rich in protein can reach 50% left and right with dry weight basis, produce the good algae of protein and contain VITAMIN, biological polyoses, unsaturated fatty acids, chlorophyll, carotenoid, is shrimp seedling, juvenile crab shellfish, the best natural bait of fry, and natural immunity material and growth-promoting substance in chlorella protein, be that mixed feed is beyond one's reach.
2) water transfer effect
Along with the continuous rising of water temperature, water body easily breaks out blue-green algae under acidic conditions, brings harm easily to shrimp crab.In breeding process, use chlorella, it is played a leading role in water body, can effectively suppress the generation of blue-green algae, reduce to cultivate risk.
3) detoxification
Chlorella is to increasing water body dissolved oxygen, ammonia nitrogen, H in degradation water
2the toxin such as S, nitrite, has special efficacy.
Under the environment of heavy metal contamination, algae can be induced to produce metal binding protein and the metallothionein(MT) MT of a kind of plant chelating, and these two kinds of albumen detoxify in conjunction with poisonous metal ion by polypeptide.
4) increase dissolved oxygen in water
The photosynthesis of chlorella is the decades of times of other plant, and the strength of absorbing sun power is outstanding, is described as " the filling sun ", is one of biology that known photosynthesis is the strongest.So chlorella has important effect for the dissolved oxygen in pond.
Extensive above-mentioned vital role based on chlorella, demand to chlorella on market is larger, therefore need to carry out concentrating and separating to chlorella, make concentrated solution, easy to use, as breeding bait, can reduce expenses, reduce risk, avoid raiser because of climate or other factors impact, algae water-mass density is rare or cultivate and unsuccessfully cause entire cost and waste of time.But the collection of micro-algae and purifying have restricted micro-algae and have cultivated extensive amplification and industrial applications.The collection method of current micro-algae mainly comprises: magnetic resolution, flocculation, air supporting etc.
Chinese patent CN102277300A discloses a kind of magnetic separation method of chlorella, and described method comprises: make Fe
3o4 nanoparticulate dispersed forms stable suspension; In chlorella nutrient solution, add described suspension, stir by the separated polymkeric substance that obtains chlorella and magnetic medium of magnetic; Described polymkeric substance, after washing, adds hydrochloric acid reaction, finishes rear micro-filtration, washing and collects filtrate.
Chinese patent CN101095459A discloses a kind of method of utilizing the concentrated marine microalgae of flocculence, it is to the aqueous solutions of organic acids that adds chitosan in micro algae culturing liquid, the concentration that makes chitosan in micro algae culturing liquid is 0.3 ~ 8.0mg/L, then the pH value that regulates micro algae culturing liquid is 4.5 ~ 8.5, make micro-algae flocculation, and adopt static layering or air-float method to collect throw out.Generally, adopt flucculation process separation and collect micro-algae and need to use various magnetic resolution media, and magnetic resolution medium and micro-algae separation difficulty easily cause the pollution of target product.
Dissolved air flotation needs the auxiliary of magnetic resolution medium or tensio-active agent conventionally while applying in micro-algae, therefore exist and the same problem of flucculation process.
Summary of the invention
The invention provides a kind of production method of chlorella concentrated solution, the thickening efficiency that overcomes existing chlorella concentration method existence is low, easily causes chlorella to pollute, and destroys the shortcomings such as chlorella nutritive substance.
The present invention adopts following technical scheme:
A production method for micro-algae concentrated solution, comprises the following steps:
(1) filter
The chlorella nutrient solution that is 50 ~ 1200mg/L by concentration carries out filtering separation, removes coarse particles, impurity in nutrient solution;
(2) concentrated
By the nutrient solution after filtering, through separated water-dialyzing and the concentrated solution of obtaining of membrane concentration, water-dialyzing is back in the cultivation pool of chlorella.
Step is filtered into a kind of of screen filtration or fine fack described in (1); Described screen filtration is 50 ~ 100 orders.
The described simmer down to micro-filtration of step (2) or ultrafiltration a kind of, the membrane module of described micro-filtration is a kind of of dull and stereotyped, tubular type or rolling, aperture is the microfiltration membrane of 0.1～0.2 micron; The mould material of described ultrafiltration is a kind of of ceramic membrane, metallic membrane or organic membrane; The membrane module of described ultrafiltration is a kind of of board-like, tubular type, pillar; The molecular weight cut-off of ultra-filtration membrane is 1000～50000MWCO, and working conditions is: normal temperature～45 ℃, and the aperture of ceramic membrane is 50 ~ 200nm, the operating pressure of plate-type hyperfiltration, tubular type ultrafiltration and pillar ultrafiltration is 3～300kPa.
From the above-mentioned description to structure of the present invention, compared to the prior art, tool of the present invention has the following advantages:
The present invention is particularly suitable for the production method of chlorella concentrated solution, by the combination of filtration, micro-filtration/hyperfiltration process, uses, and thickening efficiency is high, do not have flocculation agent residue problem, prepared chlorella concentrated solution purity is high, steady quality, chlorella content is high, and the shelf time is long.
Accompanying drawing explanation
Fig. 1 is preparation flow figure of the present invention.
Embodiment
1 the specific embodiment of the present invention is described with reference to the accompanying drawings.
Embodiment 1
(1) filter
The chlorella nutrient solution that is 50mg/L by concentration carries out filtering separation, removes coarse particles, impurity in nutrient solution; The described screen filtration that is filtered into, screen cloth used is 75 orders.
(2) concentrated
Nutrient solution after filtering is carried out to membrane sepn and obtain water-dialyzing and concentrated solution, water-dialyzing is back in the cultivation pool of chlorella, and the concentration of gained chlorella concentrated solution is 10g/L; Described simmer down to ultrafiltration and concentration; The mould material of described ultrafiltration is ceramic membrane, and the aperture of ceramic membrane is 200nm.
Embodiment 2
(1) filter
The chlorella nutrient solution that is 600mg/L by concentration carries out filtering separation, removes coarse particles, impurity in nutrient solution; Described filtration unit adopts fine fack.
(2) concentrated
Nutrient solution after filtering is carried out to membrane sepn and obtain water-dialyzing and concentrated solution, water-dialyzing is back in the cultivation pool of chlorella, and the concentration of gained chlorella concentrated solution is 48g/L; Described concentrating unit is micro-filtration; The membrane module of described micro-filtration is flat sheet membrane, and aperture is 0.1 micron.
Embodiment 3
(1) filter
The chlorella nutrient solution that is 1200mg/L by concentration carries out filtering separation, removes coarse particles, impurity in nutrient solution; Described filtration unit adopts fine fack.
(2) concentrated
Nutrient solution after filtering is carried out to membrane sepn and obtain water-dialyzing and concentrated solution, water-dialyzing is back in the cultivation pool of chlorella, and the concentration of gained chlorella concentrated solution is 60g/L; Described simmer down to ultrafiltration and concentration; The mould material of described ultrafiltration is ceramic membrane, and the aperture of ceramic membrane is 50nm.
Above are only the specific embodiment of the present invention, but design concept of the present invention is not limited to this, allly utilizes this design to carry out the change of unsubstantiality to the present invention, all should belong to the behavior of invading protection domain of the present invention.
Claims (7)
1. a production method for chlorella concentrated solution, it comprises the following steps:
(1) filter
The chlorella nutrient solution that is 50 ~ 1200mg/L by concentration carries out filtering separation, removes coarse particles, impurity in nutrient solution;
(2) concentrated
Nutrient solution after filtering is carried out to membrane sepn and obtain water-dialyzing and concentrated solution, water-dialyzing is back in the cultivation pool of chlorella.
2. the production method of a kind of chlorella concentrated solution as claimed in claim 1, is characterized in that: described in step (1), be filtered into a kind of that screen filtration or fine fack filter.
3. the production method of a kind of chlorella concentrated solution as claimed in claim 2, is characterized in that: the screen cloth of the described screen filtration of step (1) is 50 ~ 100 orders.
4. the production method of a kind of chlorella concentrated solution as claimed in claim 1, is characterized in that: the described simmer down to micro-filtration of step (2) or ultrafiltration a kind of.
5. the production method of a kind of chlorella concentrated solution as claimed in claim 4, is characterized in that: the membrane module of the described micro-filtration of step (2) is a kind of for flat board, tubular type or rolling, and aperture is 0.1～0.2 micron.
6. the production method of a kind of chlorella concentrated solution as claimed in claim 4, it is characterized in that: the mould material of the described ultrafiltration of step (2) is a kind of of ceramic membrane, metallic membrane or organic membrane, the molecular weight cut-off of ultra-filtration membrane is 1000～50000MWCO, working conditions is: normal temperature～45 ℃, the aperture of ceramic membrane is 50～200nm.
7. the production method of a kind of chlorella concentrated solution as claimed in claim 4, is characterized in that: the membrane module of the described ultrafiltration of step (2) is a kind of for flat board, tubular type or rolling, and the operating pressure of pillar ultrafiltration, tubular type ultrafiltration and plate-type hyperfiltration is 3～300kPa.
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| CN201410031621.6A CN103740595A (en) | 2014-01-23 | 2014-01-23 | Method for producing chlorella concentrated solution |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104450523A (en) * | 2014-11-29 | 2015-03-25 | 黄南概 | Preparation method of chlorella concentrated solution |
| CN105779293A (en) * | 2016-05-18 | 2016-07-20 | 中国计量大学 | Chlorella preservation method |
| CN108504580A (en) * | 2018-04-19 | 2018-09-07 | 甘肃德福生物科技有限公司 | A kind of preparation method of the fresh and alive chlorella suspension improved the immunity of the human body |
| CN108587912A (en) * | 2018-04-12 | 2018-09-28 | 内蒙古科技大学 | A kind of Dunaliella salina industrialized production collecting method |
| CN109527191A (en) * | 2018-11-27 | 2019-03-29 | 北海生巴达生物科技有限公司 | The technique that drink or small molecule Gly-His-Lys are prepared using peptide molecular weight selection technique |
| CN109837203A (en) * | 2017-11-28 | 2019-06-04 | 浙江太阳石水处理有限公司 | A kind of efficient microalgae concentration technology based on ceramic membrane technology |
| CN112881632A (en) * | 2021-01-20 | 2021-06-01 | 深圳市水文水质中心 | Method and device for counting algae in water sample |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103468399A (en) * | 2013-09-24 | 2013-12-25 | 北京化工大学 | Method for extracting microbial oil |
-
2014
- 2014-01-23 CN CN201410031621.6A patent/CN103740595A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103468399A (en) * | 2013-09-24 | 2013-12-25 | 北京化工大学 | Method for extracting microbial oil |
Non-Patent Citations (3)
| Title |
|---|
| 周玮等: "单胞藻超滤浓缩技术研究与发展", 《大连水产学院学报》 * |
| 宫庆礼等: "超滤技术在单胞藻浓缩中的应用", 《实验与技术》 * |
| 徐跃定等: "小球藻规模化生产采收技术", 《江苏农业科学》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104450523A (en) * | 2014-11-29 | 2015-03-25 | 黄南概 | Preparation method of chlorella concentrated solution |
| CN105779293A (en) * | 2016-05-18 | 2016-07-20 | 中国计量大学 | Chlorella preservation method |
| CN105779293B (en) * | 2016-05-18 | 2019-11-22 | 中国计量大学 | A kind of store method of chlorella |
| CN109837203A (en) * | 2017-11-28 | 2019-06-04 | 浙江太阳石水处理有限公司 | A kind of efficient microalgae concentration technology based on ceramic membrane technology |
| CN108587912A (en) * | 2018-04-12 | 2018-09-28 | 内蒙古科技大学 | A kind of Dunaliella salina industrialized production collecting method |
| CN108504580A (en) * | 2018-04-19 | 2018-09-07 | 甘肃德福生物科技有限公司 | A kind of preparation method of the fresh and alive chlorella suspension improved the immunity of the human body |
| CN109527191A (en) * | 2018-11-27 | 2019-03-29 | 北海生巴达生物科技有限公司 | The technique that drink or small molecule Gly-His-Lys are prepared using peptide molecular weight selection technique |
| CN112881632A (en) * | 2021-01-20 | 2021-06-01 | 深圳市水文水质中心 | Method and device for counting algae in water sample |
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Application publication date: 20140423 |