CN108587912A - A kind of Dunaliella salina industrialized production collecting method - Google Patents
A kind of Dunaliella salina industrialized production collecting method Download PDFInfo
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Abstract
The present invention discloses a kind of Dunaliella salina industrialized production collecting method, including：Determine the type and pore size of feed pump type and filter membrane used in film concentration systems；The maximum pressure that test film condensing device used can bear determines the most suitable feed rate of film concentration technology harvesting Dunaliella salina and best cycles of concentration；Determine the harvest efficiency of different feeds rate and cycles of concentration lower film concentration harvesting Dunaliella salina technique；Determine the range of centrifuge secondary concentration technique centrifuge speed；Determine the cleaning method of film condensing device.Compared with the method for tradition harvesting Dunaliella salina, invention significantly improves the harvest efficiencies of frustule, reduce percentage of damage of the Dunaliella salina cell in recovery process, in the treating capacity for not influencing algae powder yield with increasing the unit interval under the premise of quality, the recovery process of frustule is set to be easier to control, the production cost for reducing both culturing microalgae enterprise simultaneously, can be used as Dunaliella salina recovery process and promotes the use of, be conducive to the long-run development of microalgae industry.
Description
Technical field
The present invention relates to a kind of Dunaliella salina industrialized production collecting methods, and in particular to a kind of film concentration technique harvesting Du
The method of family name salt algae.
Background technology
Dunaliella salina, Chlorophyta, true Chlorophyceae, volvocales, Yan Zao sections are algaes representative in Du Shi algaes, are
A kind of single celled eukaryotic algae of thermophilic salt, is one of the biology for finding most salt tolerant and thermophilic salt till now, Dunaliella salina is without thin
Cell wall structures, being stimulated by extraneous osmotic pressure variation makes the variable range of its cell length be 2-29 μm, and variable-width is ranging from
1-15 μm.Dunaliella salina can accumulate a large amount of carotenoid under stress conditions, and wherein content beta-carotene is most, and highest can
Account for the 14% of its dry weight.Beta carotene category tetraterpenes compound is the precursor of vitamin A, has very strong scavenging activated oxygen
Function, can anti-curing cancers, tumour and angiocardiopathy and the degeneration related with the age such as senile dementia and cataract
Disease, therefore additive is can be used as food service industry and cosmetic industry.Meanwhile beta carotene is a kind of important health care
Product are widely present in all kinds of health foods and drug.A variety of answer has been made in the carrotene extracted from salt algae at present
For radioresistance, protection eyesight, the nutriment for alleviating asthenopia.Dunaliella salina has very high market value, cultivates Du Shi
Yan Zao manufacturing enterprises are also more and more.
The recovery process used in country's Dunaliella salina breeding enterprise is largely centrifugal separation at present.In microalgae separation
For the disc centrifugal separator of automatic clinker-removing using more, this kind of seperator is easily operated, and can continuous work；Due to Dunaliella salina
Cell has flagellum, and without cell wall, in centrifuging recovery process, Dunaliella salina cell is influenced by high shear force, holds
Broken, flagellum is easy to fall off, and the cell that integrality is destroyed is difficult enrichment in tube centrifuge and butterfly centrifugal machine, this
It is relatively low that frustule harvest efficiency will be directly resulted in；Meanwhile broken frustule after centrifugation return water to cultivation raceway pond in, by
It leaks outside in pigment, leads to algae solution color burn, be unfavorable for the photosynthesis of normal frustule in algae solution；In addition, tube centrifuge
Or butterfly centrifugal machine is expensive, energy consumption is higher, and treatment effeciency is relatively low, and common tube centrifuge can only be handled for every per hour
1m3The algae solution of volume, this can be such that Dunaliella salina recovery process cost greatly improves, and therefore, Dunaliella salina breeding enterprise compels to be essential
Seek the recovery process for finding that a kind of effect is more preferable, energy consumption is lower, more efficient.
Invention content
Meaning of the present invention is to provide a kind of Dunaliella salina industrialized production collecting method；The effect of film concentration technology is former
Reason is, under the action of hydraulic pressure, filter membrane allows H2O and inorganic salts etc. pass through, but can retain suspended matter, bacterium and frustule etc.
Substance, crown_interception make frustule be limited in filter membrane surface, as algae solution is constantly pumped to membranous system, trapped substance can it is more long-pending more
It is more, so as to constantly be discharged in specific container, and by hydrone can be discharged into the inorganic matter being dissolved in hydrone
In raceway pond, achieve the purpose that cycling and reutilization；Centrifuge secondary concentration is carried out on the basis of film concentrates, and can be further increased
The cycles of concentration of algae solution keeps concentrated effect more preferable, is more convenient for carrying out downstream processes to algal gel；It is detached with conventional centrifugal used in enterprise
Method is compared, and the film concentration technology in the method for the invention has continuous processing amount big, easy to control, and harvest efficiency is high,
The features such as low energy consumption, and return water does not influence normal frustule growth in raceway pond, and the technique is to Dunaliella salina cellular damage degree
It is minimum, it is advantageous for carrying out centrifuge secondary concentration；Therefore the consumption of Dunaliella salina breeding enterprise recovery process is greatly reduced
Time and cost increase substantially cell harvesting efficiency under the premise of not influencing algae silty amount with yield, business economic are made to imitate
Benefit increases obviously, significant for the development of Dunaliella salina aquaculture.In order to achieve the above objectives, the technical side that the present invention uses
Case is broadly divided into following five part.
A kind of Dunaliella salina industrialized production collecting method, includes the following steps：
First part：Determine the type and pore size of feed pump type and filter membrane used in film concentration systems；
Second part：Determine the most suitable feed rate of film concentration technology harvesting Dunaliella salina and best cycles of concentration；Concrete operations
Step is：The pressure limit that film condensing device used can bear is tested first；Then it can bear according to film condensing device
Maximum pressure determines feed rate range and under different feed rates, shuts out after algae solution is concentrated into different multiples respectively
The percentage of damage of family name's Dunaliella salina cell, percentage of damage calculating process are as follows：
1. the cell number a before determining concentration in former algae solution；
2. after determining that original algae solution is concentrated into certain multiple c, the complete cell number b of morphosis in algae solution is concentrated；
3. Dunaliella salina cell crashing ratio R calculation formula are as follows after algae solution is concentrated into different multiples：
R=(ac-b)/ac × 100%
4. determining different feeds amount and the complete frustule number d of morphosis in cycles of concentration lower water-back；
Part III：Determine the harvest efficiency of different feeds rate and cycles of concentration lower film concentration harvesting Dunaliella salina technique;It adopts
Rate of producing effects calculation formula is as follows：
Part IV：Determine the range of secondary concentration technique centrifuge speed；
Part V：The cleaning method for determining film condensing device, the part return water generated after film is concentrated is as film condensing device
The cleaning agent of middle filter membrane, film condensing device four hours that often work are cleaned once with the mode that first square impact recoils again, flushing degree
Until the liquid-transparent of outflow.
Further, the feed pump selected in the first part is screw pump, by the intermeshing of screw rod when work
And the tight fit of screw rod and lining inner wall, it is broken to be avoided that algae solution frustule structure during by feed pump is sheared power
It is bad, the percentage of damage that algae solution enters frustule before film condensing device is greatly lowered.
Further, the micro-filtration that filter membrane is 0.1-0.8 μm using aperture in first part described in the first part
The variable range of film, Dunaliella salina cell length is 2-29 μm, and ranging from 1-15 μm of variable-width, filter sizes are Dunaliella salina cell
It, within this range, can be under the premise of retaining all Dunaliella salina cells within length and width variable range, H2O and inorganic salts can be with
Quickly through this guarantees the processing speeds of film condensing device.
Further, the water flowing at the feed inlet of film condensing device is slowly increased the rate of flow, until to break through film dense for flow
Compression apparatus in a flash, the pressure of water under this flow rate regime is determined according to pressure gauge, in this pressure limit, for film condensing device institute
The pressure limit that can be born, the pressure limit that can bear according to film condensing device can obtain feed rate range, test institute
With film condensing device allowable stress range, the device can be protected to be destroyed from hydraulic pressure, ensure film concentration harvesting Dunaliella salina mistake
Cheng Chixu is efficiently carried out.
Further, the second part is counted by light microscope microscopy with blood counting chamber, determines different feeds
Rate and the complete frustule number of morphosis in cycles of concentration lower water-back obtain membrane concentration according to harvest efficiency calculation formula
The harvest efficiency of technique, while effectively evaluating is made to the recyclable property of return water.
Further, the Part IV, the ranging from 4000-10000r/min of secondary concentration technique centrifuge speed；Du
Family name's Dunaliella salina cell detaches under centrifugation force effect of the rotating speed more than 4000 r/min with culture medium, and rotating speed is more than 10000r/
Min, the horizontal direction shearing force generated by centrifugal force can be such that frustule is crushed, and energy consumption accordingly can also increase, can be according to enterprise's need
The range of speeds of centrifuge when choosing whether to carry out centrifuge secondary concentration, and determining secondary concentration.
Further, the Part V uses the return water that film concentration generates as film condensing device cleaning agent, can avoid harmful
The use of chemical prevents from having an adverse effect to product quality；Filter membrane is carried out with backwash two ways using just washing
Cleaning can fully wash the concentration frustule being trapped in filter membranous layer, flow into concentration frustule collection device, harvesting is made to imitate
Fruit is more thorough.
Further, the return water periodically generated using film concentration carries out film condensing device in such a way that first square impact recoils again clear
Clean maintenance can prevent filter membrane cooperating microorganisms, extend the service life of filter membrane, ensure the continuous service of film condensing device.
The beneficial effects are mainly as follows：
（1）Feed pump is screw pump used in film concentration harvesting Dunaliella salina technique；Dunaliella salina cell does not have cell wall structure,
The shearing force of other pumps can be such that frustule structure destroys, this will have a direct impact on the harvest efficiency of film concentration technology；Screw pump makes
With, it is avoided that algae solution frustule structure during by feed pump is sheared power destruction, intracellular beta carotene is avoided to be lost in,
The percentage of damage that algae solution enters frustule before film condensing device is greatly lowered, ensures centrifuge secondary concentration and follow-up desalinization of soil by flooding or leaching process
Effective progress, prevent the beta carotene of the broken frustule and loss after the desalinization of soil by flooding or leaching in return water to raceway pond, due to its color compared with
The photosynthesis of normal cell in raceway pond can be influenced deeply, ensure algae silty amount；
（2）The action principle of film concentration technology is under the action of hydraulic pressure, and filter membrane film allows H2O and inorganic salts etc. pass through, but meeting
Suspended matter is retained, the substances such as bacterium and frustule, crown_interception is limited in filter membrane surface, as algae solution is constantly pumped to film
System, trapped substance can be cumulative, so as to constantly be discharged in specific container, and by hydrone and be dissolved in moisture
Inorganic matter in son can be discharged into raceway pond, achieve the purpose that recycling；And the film concentration systems for harvesting Dunaliella salina are adopted
It it is 0.1-0.8 μm with the pore diameter range of microfiltration membranes, under the microfiltration membranes effect in the aperture, Dunaliella salina cell can be retained all
With on film, and can effectively drop, it can be achieved that return water recycles after harvesting in the raceway pond that culture medium then can be after return water to harvesting
The usage amount of inorganic nutrient salt needed for low culture medium saves production cost；
（3）Return water makees cleaning agent after film condensing device selects concentration, and square impact and the comprehensive mode used of recoil carry out cleaning maintenance,
The cleaning way of film condensing device is divided into two kinds of square impact and recoil；Square impact and recoil two ways synthesis use, can be on filter membrane
Retentate fully clean up；These retentates are the frustule group of concentration, these retentates, which are flushed to concentration frustule, to be collected
In device, keep harvesting Dunaliella salina more thorough；Due to the brine that Dunaliella salina culture medium is high salinity, algae solution is concentrated
The return water part generated afterwards carrys out cleaning membrane system as cleaning agent, which can effectively prevent filter membrane as the brine of high salinity
Cooperating microorganisms；
（4）Film concentration harvesting Dunaliella salina technique have it is minimum to Dunaliella salina cellular damage degree, batch processed amount is big, operation
Be easy to control, harvest efficiency is high, and low energy consumption, ensures that desalinization of soil by flooding or leaching step lasts in downstream effectively carry out, after harvesting return water with after the desalinization of soil by flooding or leaching
Return water does not influence the advantages that normal frustule growth in raceway pond, while centrifuge secondary concentration can further increase the dense of algae solution
Demagnification number keeps concentrated effect more preferable, is more convenient for carrying out downstream processes to algal gel.Therefore Dunaliella salina breeding enterprise is greatly reduced
The time of recovery process consumption and cost increase substantially cell harvesting effect under the premise of not influencing algae silty amount with yield
Rate makes Business Economic Benefit increase apparent, significant to the development of Dunaliella salina aquaculture.
Description of the drawings
Fig. 1 is former algae solution and different feeds rate and the cell number in concentration algae solution under cycles of concentration.
Fig. 2 is the percentage of damage of different feeds rate and frustule in concentration process under cycles of concentration.
Specific implementation mode：
Following embodiment is for illustrating the present invention, convenient for being better understood from the present invention, but is not used in the limitation present invention, this reality
The common technology that experimental technique involved in example is well known to those skilled in the art, experiment material and reagent are applied, it is such as not special
It does not mentionlet alone bright, is commercial goods.
In order to achieve the above objectives, the technical solution adopted by the present invention is broadly divided into following five part：
First part：Determine the type and pore size of feed pump type and film used in film concentration systems；
Second part：Determine the most suitable feed rate of film concentration technology harvesting Dunaliella salina and best cycles of concentration；Concrete operations
Step is：
（1）The pressure limit that test film condensing device used can bear；
（2）The maximum pressure that can bear according to film condensing device determines feed rate range and in different feed rates
Under, algae solution is concentrated into the percentage of damage of Dunaliella salina cell after different multiples respectively.Percentage of damage calculating process is as follows：
1. the cell number a before determining concentration in former algae solution；
2. after determining that original algae solution is concentrated into certain multiple c, the complete cell number b of morphosis in algae solution is concentrated；
3. Dunaliella salina cell crashing ratio R calculation formula are as follows after algae solution is concentrated into different multiples：
R=(ac-b)/ac × 100%
4. determining different feeds amount and the complete frustule number d of morphosis in cycles of concentration lower water-back；
Part III：Determine the harvest efficiency of different feeds rate and cycles of concentration lower film concentration harvesting Dunaliella salina technique.It adopts
Rate of producing effects calculation formula is as follows：
Part IV：Determine the range of secondary concentration technique centrifuge speed；
Part V：Determine the cleaning method of film condensing device.
Wherein first part the step of（1）, determine that feed pump used in film concentration harvesting Dunaliella salina technique is screw pump.
Wherein first part the step of（2）, determine that film concentration harvesting Dunaliella salina technique should should be using pore diameter range
0.1-0.8 μm of microfiltration membranes.
Wherein second part the step of（1）, it is 0-2.5 to test the pressure limit that film condensing device used can bear
MPa。
Wherein second part the step of（2）, determine the feed rate ranging from 1-8 m of film condensing device3/ h can use concentration
Ranging from 1-30 times of multiple.
Wherein second part the step of（2）, each processing group film concentration process returns under different feeds rate and cycles of concentration
Cell number is 0 in water.
Wherein Part III, by above-mentioned second part step（2）As a result membrane concentration harvesting Du Shi salt can be obtained according to formula
The efficiency of algae is 100%, and feed rate does not influence the harvest efficiency of the technique with cycles of concentration known to analysis.
Wherein Part IV, secondary concentration centrifuge speed ranging from 4000-10000 r/min.
The cleaning maintenance mode of wherein Part V, film condensing device is：The part return water generated after film is concentrated as
The cleaning agent of filter membrane in the device, film condensing device four hours that often work are cleaned once with the mode that first square impact recoils again, punching
Degree is washed until the liquid-transparent flowed out.
The technical solution of specific each embodiment is as follows.
Embodiment 1：Feed rate is 1 m3After original is concentrated into 10,20 and 30 times when/h, form in statistics concentration algae solution
The cell number of structural integrity calculates the cell crashing ratio of each processing group；
Experiment material and equipment：Film condensing device, blood counting chamber, ordinary optical microscope, 150mL triangular flasks, liquid-transfering gun；
Experimental program：Dunaliella salina cell in former algae solution is counted with blood counting chamber, is 15 m by volume3Algae solution,
1 m is pressed by film condensing device3/ h feed rates concentrate, with blood counting chamber respectively to being concentrated into 1.5 m3、0.75 m3With 0.5
m3Concentration after algae solution counted.Each processing group three is parallel, and final result is averaged；
Experiment condition：Voltage required by film condensing device and hydraulic pressure.
Embodiment 2：Feed rate is 2 m3After original is concentrated into 10,20 and 30 times when/h, form in statistics concentration algae solution
The cell number of structural integrity calculates the cell crashing ratio of each processing group；
Experiment material and equipment：With embodiment 1；
Experimental program：Dunaliella salina cell in former algae solution is counted with blood counting chamber, is 15 m by volume3Algae solution,
2 m are pressed by film condensing device3/ h feed rates concentrate, with blood counting chamber respectively to being concentrated into 1.5 m3、0.75 m3With 0.5
m3Concentration after algae solution counted.Each processing group three is parallel, and final result is averaged；
Experiment condition：With embodiment 1.
Embodiment 3：Feed rate is 4 m3After original is concentrated into 10,20 and 30 times when/h, form in statistics concentration algae solution
The cell number of structural integrity calculates the cell crashing ratio of each processing group；
Experiment material and equipment：With embodiment 1；
Experimental program：Dunaliella salina cell in former algae solution is counted with blood counting chamber, is 15 m by volume3Algae solution,
4 m are pressed by film condensing device3/ h feed rates concentrate, with blood counting chamber respectively to being concentrated into 1.5 m3、0.75 m3With 0.5
m3Concentration after algae solution counted.Each processing group three is parallel, and final result is averaged；
Experiment condition：With embodiment 1.
Embodiment 4：Feed rate is 6 m3After original is concentrated into 10,20 and 30 times when/h, form in statistics concentration algae solution
The cell number of structural integrity calculates the cell crashing ratio of each processing group；
Experiment material and equipment：With embodiment 1；
Experimental program：Dunaliella salina cell in former algae solution is counted with blood counting chamber, is 15 m by volume3Algae solution,
6 m are pressed by film condensing device3/ h feed rates concentrate, with blood counting chamber respectively to being concentrated into 1.5 m3、0.75 m3With 0.5
m3Concentration after algae solution counted.Each processing group three is parallel, and final result is averaged；
Experiment condition：With embodiment 1.
Embodiment 5：Feed rate is 8 m3After original is concentrated into 10,20 and 30 times when/h, form in statistics concentration algae solution
The cell number of structural integrity calculates the cell crashing ratio of each processing group；
Experiment material and equipment：With embodiment 1；
Experimental program：Dunaliella salina cell in former algae solution is counted with blood counting chamber, is 15 m by volume3Algae solution,
8 m are pressed by film condensing device3/ h feed rates concentrate, with blood counting chamber respectively to being concentrated into 1.5 m3、0.75 m3With 0.5
m3Concentration after algae solution counted.Each processing group three is parallel, and final result is averaged；
Experiment condition：With embodiment 1.
Embodiment 6：Determine the harvest efficiency of film concentration harvesting Dunaliella salina technique；
Experiment material and equipment：Blood counting chamber, ordinary optical microscope, 150 mL triangular flasks, liquid-transfering gun；
Experimental program：Dunaliella salina cell in the film concentration process return water of above-mentioned all processing groups is counted with blood counting chamber
Number, the harvest efficiency of each processing group is calculated by calculation formula.Every time three it is parallel, final result is averaged；
Experiment condition：Laboratory condition；
Experimental result：The harvest efficiency of above-mentioned all processing groups is 100%.
Examples detailed above is compared, comparing result such as Fig. 1, shown in 2, and as a result, obtaining in analysis chart：
The feed rate of film condensing device is determined that screw rod revolution speed is bigger, and feed rate is bigger by screw rod revolution speed, frustule warp
Cross the easier power of being sheared damage when pump.Feed rate is lower, and the treating capacity in the unit interval is relatively low, reaches identical cycles of concentration
It is cycle-index of the algae solution in membranous system to increase, recycle ratio is more, and the frustule the easy to be mechanically damaged.It can root
The feed rate of adjustment film condensing device is needed according to enterprise.
Film cycles of concentration is higher, and algae solution is more by the number of ultrafiltration membrane, and cell crashing ratio is bigger；And cycles of concentration is got over
Low, film condensing device processing speed will reduce；The cycles of concentration of adjustment film condensing device, joint centrifugation can be needed according to enterprise
Machine secondary concentration technique, harvests Dunaliella salina.
The size of feed rate and the height of cycles of concentration do not influence the harvesting effect of film concentration harvesting Dunaliella salina technique
Rate, under the conditions of filter membrane type and suitable aperture selection, the harvest efficiency of the technique is 100%.
The part return water that film condensing device generates after concentrating film as the cleaning agent of filter membrane, use by the 1-6 hour that often work
The mode that first square impact recoils again is cleaned once, and flushing degree can prevent filter membrane cooperating microorganisms until the liquid-transparent flowed out,
The service life for extending filter membrane, ensures the continuous service of film condensing device.
In conclusion the method for film concentration harvesting Dunaliella salina, selects screw pump for feed pump, in 0-2.5 MPa pressure
In range, select aperture for 0.1-0.8 μm of microfiltration membranes on the basis of, feed rate usable range be 1-8 m3/ h can use concentration
Ranging from 1-30 times of multiple, harvest efficiency is high, and low energy consumption, under the conditions of different feed rates and cycles of concentration, to Du Shi
The degree of injury of Dunaliella salina cell is smaller；Centrifuge secondary concentration can be carried out as needed after film concentration, and centrifuge speed is ranging from
4000-10000 r/min；Meanwhile generating return water with film concentration frustule（Dunaliella salina used medium is high salinity brine）
It often works four hours as the device and carries out square impact and the cleaning agent of recoil；Therefore, the method for film concentration harvesting Dunaliella salina can
It is used as the recovery process of Dunaliella salina breeding enterprise, and can be promoted and applied.
It should be noted that any equivalent way that those skilled in the art are made under the introduction of this specification, or
Obvious variant should all be within the scope of the present invention.
Claims (6)
1. a kind of Dunaliella salina industrialized production collecting method, including following five parts：
First part：Determine the type and pore size of feed pump type and filter membrane used in film concentration systems；
Second part：Determine the feed rate and cycles of concentration of film concentration technology harvesting Dunaliella salina；Concrete operation step is：It is first
First test the pressure limit that film condensing device used can bear；Then the maximum pressure that can bear according to film condensing device,
It determines feed rate range and under different feed rates, algae solution is concentrated into Dunaliella salina cell after different multiples respectively
Percentage of damage, percentage of damage calculating process is as follows：
1. the cell number a before determining concentration in former algae solution；
2. after determining that original algae solution is concentrated into certain multiple c, the complete cell number b of morphosis in algae solution is concentrated；
3. Dunaliella salina cell crashing ratio R calculation formula are as follows after algae solution is concentrated into different multiples：
R=(ac-b)/ac × 100%
4. determining different feeds amount and the complete frustule number d of morphosis in cycles of concentration lower water-back；
Part III：Determine the harvest efficiency of different feeds rate and cycles of concentration lower film concentration harvesting Dunaliella salina technique;It adopts
Rate of producing effects calculation formula is as follows：
Part IV：Determine the range of secondary concentration technique centrifuge speed；
Part V：The cleaning method for determining film condensing device, the part return water generated after film is concentrated is as film condensing device
The cleaning agent of middle filter membrane, film condensing device four hours that often work are cleaned once with the mode that first square impact recoils again, flushing degree
Until the liquid-transparent of outflow.
2. a kind of Dunaliella salina industrialized production collecting method according to claim 1, it is characterised in that：Described first
The feed pump selected in point is screw pump.
3. a kind of Dunaliella salina industrialized production collecting method according to claim 1, it is characterised in that：Described first
The microfiltration membranes that filter membrane is 0.1-0.8 μm using aperture in point.
4. a kind of Dunaliella salina industrialized production collecting method according to claim 1, it is characterised in that：It concentrates and fills in film
Water flowing at the feed inlet set is slowly increased the rate of flow, until flow breaks through film condensing device in a flash, according to pressure gauge
Determine the pressure of water under this flow rate regime, it is dense according to film for the pressure limit that film condensing device can bear in this pressure limit
The pressure limit that compression apparatus can bear can obtain feed rate range.
5. a kind of Dunaliella salina industrialized production collecting method according to claim 1, it is characterised in that：Described second
Point, it by light microscope microscopy, is counted with blood counting chamber, determines different feeds rate and the shape in cycles of concentration lower water-back
The frustule number of state structural integrity, the harvest efficiency of membrane concentration technology is obtained according to harvest efficiency calculation formula, while to return water
Recyclable property make evaluation.
6. a kind of Dunaliella salina industrialized production collecting method according to claim 1, it is characterised in that：Described 4th
Point, the range of secondary concentration technique centrifuge speed is 4000-10000r/min；Dunaliella salina cell is more than 4000 in rotating speed
It is detached with culture medium under the centrifugation force effect of r/min.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010233551A (en) * | 2009-03-31 | 2010-10-21 | Shizuoka Prefecture | Method for culturing microalga |
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