CN102690757A - Keep-alive concentration method for chrysophyceae and device for implementing method - Google Patents
Keep-alive concentration method for chrysophyceae and device for implementing method Download PDFInfo
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- CN102690757A CN102690757A CN2012101714870A CN201210171487A CN102690757A CN 102690757 A CN102690757 A CN 102690757A CN 2012101714870 A CN2012101714870 A CN 2012101714870A CN 201210171487 A CN201210171487 A CN 201210171487A CN 102690757 A CN102690757 A CN 102690757A
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Abstract
The invention discloses a keep-alive concentration method for a chrysophyceae and a device for implementing the method. The invention has the advantages that through the adoption of the method and the device, the keep-alive ratio of the chrysophyceae reaches above 95% within 24 h after the chrysophyceae is concentrated to 30 to 100 million cells per liter. According to the keep-alive concentration method for the chrysophyceae, an external compression super-microfiltration membrane is adopted to concentrate to-be-concentrated chrysophyceae solution to obtain concentrated chrysophyceae solution, wherein the super-microfiltration membrane is a polyvinylidene fluoride super-microfiltration membrane with an aperture of less than 0.05 micron. The device for implementing the method comprises an injection pump, a chrysophyceae solution storage tank, a chrysophyceae solution pump, an external compression super-microfiltration membrane component, a back-flushing pump and a liquid waste storage tank, wherein the injection pump is communicated with the chrysophyceae solution storage tank by a first pipeline; the chrysophyceae solution storage tank is further communicated with the inlet of the external compression super-microfiltration membrane component by a second pipeline; and the liquid waste outlet of the external compression super-microfiltration membrane component is connected with a third pipeline inlet and a fourth pipeline outlet in parallel.
Description
Technical field
The present invention relates to a kind of little algae concentrating means and device thereof, particularly relate to spissated method of a kind of chrysophyceae keep-alive and device thereof.
Background technology
Chrysophyceae is an indispensable bait in the present aquatic animal seed production process.If directly will be used for producing and have following problem: 1. can improve the animal lymph kind and culture the toxic compositions such as ammonia nitrogen in the water body, have a strong impact on growing of cultivated animals without spissated chrysophyceae cultivation stoste; 2. the chrysophyceae cell density is very low in the cultivation stoste, and is high as the production marketing transportation cost, can't carry out specialization and produce; 3. can't process and store chrysophyceae bait.
Culturing microalgae bait is concentrated and collects is the gordian technique point in the biological feed production process always, also is the major portion that the Mirco algal food product cost constitutes.At present little algae being carried out spissated main method has: centrifuging, flocculence, filtration method, air supporting method etc.Conventional micro-filtration and ultra filtration cause little algae to stop up filter opening usually and make filtration procedure be difficult to continue carry out; Even more serious is; Chrysophyceae is a kind of unicellular algae that does not have cell walls, in traditional ultra-filtration process, can cause necrocytosis even breaks and lose the bait using value.
Summary of the invention
The technical problem that the present invention will solve provides spissated method of a kind of chrysophyceae keep-alive and device thereof, uses this method and apparatus, and after chrysophyceae was concentrated into 3000-10000 ten thousand cells/ml, the keep-alive rate in 24 hours reached more than 95%.
The invention provides the spissated method of a kind of chrysophyceae keep-alive, adopt the external-compression type supermicro filtration membrane to treat to concentrate chrysophyceae liquid to concentrate, obtain the chrysophyceae liquid concentrator, wherein said supermicro filtration membrane is the aperture less than 0.05 micron pvdf supermicro filtration membrane.
The used pvdf supermicro filtration membrane of the present invention is a common material in the water treatment, but is not applied to as yet during little algae keep-alive concentrates.Supermicro filtration membrane is meant the aperture less than 0.1 micron filter membrane, and aperture used in the present invention is used for the chrysophyceae keep-alive less than 0.05 micron supermicro filtration membrane and concentrates, and the keep-alive rate can reach more than 95%.
The spissated method of chrysophyceae keep-alive of the present invention is characterized in that: the working pressure when said pvdf supermicro filtration membrane uses is≤0.3Mpa, keeps transmembrane pressure≤0.2Mpa, and working temperature is 5-45 ℃.
The spissated method of chrysophyceae keep-alive of the present invention, the concentration of wherein said chrysophyceae liquid concentrator are 3000-10000 ten thousand cells/ml.
The present invention also provides a kind of successive to realize the chrysophyceae keep-alive concentrating unit of aforesaid method; Comprise injection pump, algae liquid storage tank, algae liquid pump, external-compression type supermicro filtration membrane assembly, recoil pump and waste liquid hold-up vessel; The injection pump outlet communicates with algae liquid storage tank through first pipeline; Algae liquid storage tank offers and concentrates algae liquid collection mouth; Concentrate concentrated algae liquid collection valve is installed on the algae liquid collection mouth, algae liquid storage tank also communicates with external-compression type supermicro filtration membrane assembly inlet through second pipeline, and external-compression type supermicro filtration membrane assembly waste liquid outlet is parallel with the 3rd entrance and the 4th pipe outlet; The 3rd pipe outlet and the 4th entrance communicate with the waste liquid hold-up vessel; Waste liquid hold-up vessel sidewall offers spillway, and external-compression type supermicro filtration membrane assembly concentrated solution outlet communicates with algae liquid storage tank, is separately installed with first to fourth regulating valve on first to fourth pipeline; Part on second pipeline between the algae liquid storage tank and second regulating valve also is equipped with the algae liquid pump; The part of the 4th pipeline between the 4th regulating valve and the 4th entrance also is equipped with recoil pump, and wherein the material of external-compression type supermicro filtration membrane is a pvdf, and the aperture of used pvdf is less than 0.05 micron.
The spissated method of chrysophyceae keep-alive of the present invention, wherein said algae liquid storage tank is closed.
The use of chrysophyceae keep-alive concentrating unit of the present invention:
1. after treating that spissated chrysophyceae liquid is filled with algae liquid storage tank through injection pump, start the algae liquid pump, chrysophyceae liquid gets into the supermicro filtration membrane assembly and carries out ultra filtration;
2. get back in the algae liquid storage tank through the concentrated algae liquid of ultra filtration; Algae liquid density in the algae liquid storage tank increases gradually; Waste liquid gets into the waste liquid storage tanks through the 3rd pipeline; The height of spillway position depends on how many waste liquids needs keep and be used for backwash liquid, and unnecessary waste liquid overflows through spillway drains;
3. after 1,2 steps are moved for some time continuously; Measure the density of chrysophyceae algae liquid in the algae liquid storage tank with the blood counting chamber telltale; After reaching desired density; Open and concentrate algae liquid and collect valve,, regulate concentrating the flow that algae liquid is collected valve according to the concentrated speed of the external-compression type supermicro filtration membrane assembly of 1,2 step measurements; Make rate of flow of fluid=algae liquid of the rate of flow of fluid that comes from first pipeline+come from external-compression type supermicro filtration membrane assembly concentrated solution outlet collect the effusive rate of flow of fluid of valve+algae liquid pump aspirated fluid flow velocity, just can realize the continuous collection of specific cells density liquid concentrator; Close recoil pump in the concentration process.
4. whenever concentrate 30 minutes, stop to concentrate and recoil, promptly open recoil pump, close injection pump, algae liquid pump, wash supermicro filtration membrane 15 seconds of at every turn recoiling.
Change if wait the cell density that concentrates in the algae liquid, need to regulate again concentrating the flow that algae liquid is collected valve.
Spissated method of chrysophyceae keep-alive of the present invention and equipment thereof are treated concentrated chrysophyceae liquid through the external-compression type supermicro filtration membrane and are concentrated; Through selection to supermicro filtration membrane material and aperture; Chrysophyceae keep-alive rate in concentration process in the algae liquid is reached more than 95%, and all right successive of this equipment obtains the chrysophyceae liquid concentrator of desired concn.
Below in conjunction with accompanying drawing chrysophyceae keep-alive concentrating unit of the present invention is described further.
Description of drawings
Fig. 1 is the structural representation of chrysophyceae keep-alive concentrating unit of the present invention.
Embodiment
As shown in Figure 1, chrysophyceae keep-alive concentrating unit of the present invention comprises injection pump 1, sealing algae liquid storage tank 2, algae liquid pump 3, external-compression type supermicro filtration membrane assembly 4, recoil pump 5 and waste liquid hold-up vessel 6; Injection pump 1 exports through first pipeline 71 and communicates with sealing algae liquid storage tank 2, seals algae liquid storage tank 2 and offers concentrated algae liquid collection mouth 21, concentrates concentrated algae liquid collection valve 85 is installed on the algae liquid collection mouth 21; Sealing algae liquid storage tank 2 also communicates with external-compression type supermicro filtration membrane assembly 4 inlets through second pipeline 72, and external-compression type supermicro filtration membrane assembly 4 waste liquid outlets are parallel with the 3rd pipeline 73 inlets and 74 outlets of the 4th pipeline, and 73 outlets of the 3rd pipeline and the 4th pipeline 74 inlets communicate with waste liquid hold-up vessel 6; Waste liquid hold-up vessel 6 sidewalls offer spillway 61, and external-compression type supermicro filtration membrane assembly 4 concentrated solution outlets communicate first to fourth pipeline 71 with sealing algae liquid storage tank 2; 72; Be separately installed with first to fourth regulating valve 81,82 on 73,74; 83; In the part between the algae liquid storage tank 2 and second regulating valve 82 part of algae liquid pump 3, the four pipelines 74 between the 4th regulating valve 84 and the 4th pipeline 74 inlets is installed also on 84, the second pipelines 72 recoil pump 5 also is installed; Wherein used external-compression type supermicro filtration membrane is the aperture less than 0.05 micron pvdf supermicro filtration membrane; Its working pressure is≤0.3Mpa, keeps transmembrane pressure to be not more than 0.2Mpa, and working temperature is 5-45 ℃ and all can.
Use aforesaid device according to following method:
1. after treating that spissated chrysophyceae liquid is filled with algae liquid storage tank through injection pump, start the algae liquid pump, chrysophyceae liquid gets into the supermicro filtration membrane assembly and carries out ultra filtration;
2. get back in the algae liquid storage tank through the concentrated algae liquid of ultra filtration, the algae liquid density in the algae liquid storage tank increases gradually, and waste liquid gets into the waste liquid storage tanks through the 3rd pipeline, and unnecessary waste liquid overflows through spillway drains;
3. after 1,2 steps are moved for some time continuously; Measure the density of chrysophyceae algae liquid in the algae liquid storage tank with the blood counting chamber telltale; After reaching desired density; Open and concentrate algae liquid and collect valve,, regulate concentrating the flow that algae liquid is collected valve according to the concentrated speed of the external-compression type supermicro filtration membrane assembly of 1,2 step measurements; Make rate of flow of fluid=algae liquid of the rate of flow of fluid that comes from first pipeline+come from external-compression type supermicro filtration membrane assembly concentrated solution outlet collect the effusive rate of flow of fluid of valve+algae liquid pump aspirated fluid flow velocity, just can realize the continuous collection of specific cells density liquid concentrator; Close recoil pump in the concentration process.
4. whenever concentrate 30 minutes, stop to concentrate and recoil, promptly open recoil pump, close injection pump, algae liquid pump, wash supermicro filtration membrane 15 seconds of at every turn recoiling.
Use said apparatus that chrysophyceae is cultivated following 24 hours of the condition that stoste is concentrated into 3000-10000 ten thousand cells/ml and is stored in 8-20 ℃; Adopt sediments microscope inspection to concentrate chrysophyceae in the chrysophyceae liquid concentrator; The chrysophyceae of motion is the chrysophyceae that lives, and its keep-alive rate all reaches more than 95%.
Above-described embodiment describes preferred implementation of the present invention; Be not that scope of the present invention is limited; Design under the prerequisite of spirit not breaking away from the present invention; Various distortion and improvement that those of ordinary skills make technical scheme of the present invention all should fall in the definite protection domain of claims of the present invention.
Claims (5)
1. spissated method of chrysophyceae keep-alive is characterized in that: adopt the external-compression type supermicro filtration membrane to treat to concentrate chrysophyceae liquid to concentrate, obtain the chrysophyceae liquid concentrator, wherein said supermicro filtration membrane is the aperture less than 0.05 micron pvdf supermicro filtration membrane.
2. the spissated method of chrysophyceae keep-alive according to claim 1 is characterized in that: the working pressure when said pvdf supermicro filtration membrane uses is≤0.3Mpa, keeps transmembrane pressure≤0.2Mpa, and working temperature is 5-45 ℃.
3. the spissated method of chrysophyceae keep-alive according to claim 1 and 2 is characterized in that: the concentration of said chrysophyceae liquid concentrator is 3000-10000 ten thousand cells/ml.
4. realize the chrysophyceae keep-alive concentrating unit of one of claim 1-3 said method; It is characterized in that: comprise injection pump (1), algae liquid storage tank (2), algae liquid pump (3), external-compression type supermicro filtration membrane assembly (4), recoil pump (5) and waste liquid hold-up vessel (6); Injection pump (1) outlet communicates with algae liquid storage tank (2) through first pipeline (71), and algae liquid storage tank (2) offers and concentrates algae liquid collection mouth (21), and concentrated algae liquid is collected concentrated algae liquid collection valve (85) is installed on mouthful (21); Algae liquid storage tank (2) also communicates with external-compression type supermicro filtration membrane assembly (4) inlet through second pipeline (72); External-compression type supermicro filtration membrane assembly (4) waste liquid outlet is parallel with the 3rd pipeline (73) inlet and the 4th pipeline (74) outlet, and the 3rd pipeline (73) outlet and the 4th pipeline (74) inlet communicate with waste liquid hold-up vessel (6), and waste liquid hold-up vessel (6) sidewall offers spillway (61); External-compression type supermicro filtration membrane assembly (4) concentrated solution outlet communicates with algae liquid storage tank (2); First to fourth pipeline (71,72,73; 74) be separately installed with first to fourth regulating valve (81 on; 82,83,84); The part that is positioned on second pipeline (72) between algae liquid storage tank (2) and second regulating valve (82) also is equipped with algae liquid pump (3); The part that the 4th pipeline (74) is positioned between the 4th regulating valve (84) and the 4th pipeline (74) inlet also is equipped with recoil pump (5), and wherein the material of external-compression type supermicro filtration membrane is a pvdf, and the aperture of used pvdf is less than 0.05 micron.
5. the spissated device of chrysophyceae keep-alive according to claim 4 is characterized in that: said algae liquid storage tank (2) is for closed.
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| CN201210171487.0A CN102690757B (en) | 2012-05-29 | 2012-05-29 | Keep-alive concentration method for chrysophyceae and device for implementing method |
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| CN201210171487.0A CN102690757B (en) | 2012-05-29 | 2012-05-29 | Keep-alive concentration method for chrysophyceae and device for implementing method |
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| CN102690757B CN102690757B (en) | 2014-05-07 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108587912A (en) * | 2018-04-12 | 2018-09-28 | 内蒙古科技大学 | A kind of Dunaliella salina industrialized production collecting method |
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| CN1333067C (en) * | 2004-11-23 | 2007-08-22 | 山东省海水养殖研究所 | Method for preparing dried chlamydomonas products, its products and use thereof |
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| CN1333067C (en) * | 2004-11-23 | 2007-08-22 | 山东省海水养殖研究所 | Method for preparing dried chlamydomonas products, its products and use thereof |
| CN200997874Y (en) * | 2007-02-05 | 2008-01-02 | 周玮 | Unicellular algae concentrator |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108587912A (en) * | 2018-04-12 | 2018-09-28 | 内蒙古科技大学 | A kind of Dunaliella salina industrialized production collecting method |
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Address after: 264006 Yaqian village, Chaoshui, economic and Technological Development Zone, Yantai City, Shandong Province Patentee after: Shandong Anyuan Seed Technology Co., Ltd Address before: Penglai City, the town of Shandong village Nga 265617 city of Yantai Province Patentee before: SHANDONG ANYUAN AQUACULTURE Co.,Ltd. |