CN103739730A - Simple preparation method of crude sargassum hornori polysaccharide - Google Patents
Simple preparation method of crude sargassum hornori polysaccharide Download PDFInfo
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- CN103739730A CN103739730A CN201310666295.1A CN201310666295A CN103739730A CN 103739730 A CN103739730 A CN 103739730A CN 201310666295 A CN201310666295 A CN 201310666295A CN 103739730 A CN103739730 A CN 103739730A
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- filter residue
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- sargassum horneri
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Abstract
The invention discloses a simple preparation method of crude sargassum hornori polysaccharide, and the simple preparation method comprises the following steps: (1) mashing sargassum hornori algae body with a dry method; (2) adding an ethanol solution with a volume concentration of above 75% for soaking for 12-24h, then filtering to obtain a filter residue; (3) boiling the filter residue at 90-95 DEG C for 1-2h according to the water and material mass ratio of 20 to 40:1, filtering to obtain a filtrate, adding water for again boiling the filter residue for one time, merging the filtrate; (4) heating and concentrating the filtrate, adding ethanol until the final volume concentration of the ethanol is 75-80%, standing for 2-5 days, and then filtering to obtain a crude polysaccharide precipitate. Compared with the prior art, the simple preparation method has the advantages of high efficiency and low cost, the application is not reported before; compared with a process of the prior art, the simple preparation method saves the cost of centrifugal equipment, and is also conducive to large-scale production, and the cost of the decolorization degreasing process is also reduced compared with the process of the prior art.
Description
Technical field
The present invention relates to the simple and easy preparation of Sargassum horneri Crude polysaccharides, belong to field of processing of aquatic products.
Background technology
Sargassum horneri (formal name used at school: Sargassum horneri) claim again post capsule sargassun, grass madder, the alizarin dish of bamboo, a kind of Japan, Korea S and north of China marine site of being distributed in, the normal common temperate zone sargassun that forms seaweed woods, can be at the beginning of annual winter Mo, spring amount reproduction, can be about one meter every day, the longlyest reach ten meters.North Taiwan seashore often has Sargassum horneri from marine site, temperate zone, to float spring, be deposited in bank and bay, 2013 because of seawater temperature lower, there is more Sargassum horneri successfully to float to Taiwan, make Sargassum horneri amount compared with being former years many, each landscaped area is all initiated the activity of clean beach a large amount of Sargassum horneris is picked up and destroyed by fire, but so a large amount of Sargassum horneris can be used as compost or seasonal food materials in fact, even develops into the raw material of the matter alcohol of making a living.
Sargassum horneri in the various and breeding of quantity nursery stage rapidly, the potential raw material as raw matter alcohol.Sargassum horneri is rich in polysaccharide in addition, after its phycocolloid extracts, can be used as anti-carcinogenic foods.
Summary of the invention
The object of this invention is to provide a kind of simple and easy method of preparing Sargassum horneri Crude polysaccharides.
In order to achieve the above object, the present invention is achieved through the following technical solutions:
A simple and easy method of preparing Sargassum horneri Crude polysaccharides, comprises the following steps:
(1) Sargassum horneri frond dry method is smashed to pieces;
(2) add the more than 75% alcohol solution dipping 12-24h of volumetric concentration, filter to obtain filter residue;
(3) according to water material mass ratio 20-40:1, filter residue is boiled to 1-2h at 90-95 ℃, cross leaching filtrate, filter residue repeats to add poach once, merging filtrate;
(4) heating concentrated filtrate, adding ethanol is 75-80% to ethanol final volume concentration, filters to obtain Crude polysaccharides precipitation after standing 2-5 days.
Preferably, step (1) by Sargassum horneri frond smash to pieces to particle diameter be 3-5mm.
Preferably, step (2), (3) and (4) adopt 200 order to 800 order filtered through gauze.
The present invention above-mentionedly prepares Sargassum horneri Crude polysaccharides method simply owing to having adopted, thereby has cost-effective advantage than prior art, before this aspect application, has no report; In addition, the long-time standing rear filtration of process using of the present invention replaces conventional centrifugal, is compared to existing technique and has saved centrifugation apparatus cost, is conducive to large-scale production simultaneously.
Embodiment
For technique means, creation characteristic that the present invention is realized, reach object and effect is easy to understand, below further set forth the present invention.
Embodiment 1:
By 600 grams of Sargassum horneri frond dry method smash to pieces to particle diameter be 3-5mm, the alcohol solution dipping 12h that adds volumetric concentration 75%, 200 order filtered through gauze obtain filter residue, filter residue water material mass ratio be 20: 1 times 90 ℃ boil 1h, 600 order filtered through gauze are got filtrate, filter residue repeats to add poach once, merging filtrate, the concentrated stoste of heating is to 3L, and adding ethanol to ethanol final volume concentration is 75%, and after standing 3d, 800 order filtered through gauze obtain Crude polysaccharides precipitation.Precipitation obtains 34.3 grams of Crude polysaccharides after vacuum lyophilization, and yield is 5.8%, and it is 15.1% that Crude polysaccharides adopts phenol sulfuric acid process to record wherein total sugar content.
Embodiment 2:
By 600 grams of Sargassum horneri frond dry method smash to pieces to particle diameter be 3-5mm, the alcohol solution dipping 20h that adds volumetric concentration 80%, 200 order filtered through gauze obtain filter residue, filter residue water material mass ratio be 30: 1 times 90 ℃ boil 1h, 400 order filtered through gauze are got filtrate, filter residue repeats to add poach once, merging filtrate, the concentrated stoste of heating is to 3L, and adding ethanol to ethanol final volume concentration is 75%, and after standing 5d, 600 order filtered through gauze obtain Crude polysaccharides precipitation.Precipitation obtains 35.1 grams of Crude polysaccharides after vacuum lyophilization, and yield is 5.9%, and it is 14.8% that Crude polysaccharides adopts phenol sulfuric acid process to record wherein total sugar content.
The above is preferred embodiment of the present invention, but the present invention should not be confined to the disclosed content of this embodiment.So every, do not depart from the equivalence completing under principles of this disclosure or revise, all falling into the scope of protection of the invention.
Claims (3)
1. a simple and easy method of preparing Sargassum horneri Crude polysaccharides, is characterized in that, comprises the following steps:
(1) Sargassum horneri frond dry method is smashed to pieces;
(2) add the more than 75% alcohol solution dipping 12-24h of volumetric concentration, filter to obtain filter residue;
(3) according to water material mass ratio 20-40:1, filter residue is boiled to 1-2h at 90-95 ℃, cross leaching filtrate, filter residue repeats to add poach once, merging filtrate;
(4) heating concentrated filtrate, adding ethanol is 75-80% to ethanol final volume concentration, filters to obtain Crude polysaccharides precipitation after standing 2-5 days.
2. the simple and easy method of preparing Sargassum horneri Crude polysaccharides according to claim 1, is characterized in that, step (1) by Sargassum horneri frond smash to pieces to particle diameter be 3-5mm.
3. the simple and easy method of preparing Sargassum horneri Crude polysaccharides according to claim 1, is characterized in that, step (2), (3) and (4) adopt 200 order to 800 order filtered through gauze.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310666295.1A CN103739730A (en) | 2013-12-10 | 2013-12-10 | Simple preparation method of crude sargassum hornori polysaccharide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310666295.1A CN103739730A (en) | 2013-12-10 | 2013-12-10 | Simple preparation method of crude sargassum hornori polysaccharide |
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| CN103739730A true CN103739730A (en) | 2014-04-23 |
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| CN201310666295.1A Pending CN103739730A (en) | 2013-12-10 | 2013-12-10 | Simple preparation method of crude sargassum hornori polysaccharide |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3930509B2 (en) * | 2004-12-21 | 2007-06-13 | 定良 秋月 | A method for collecting a viscous extract containing fucoidan from the brown alga Akamok (scientific name: Sargassumhorneri). |
| JP2011202080A (en) * | 2010-03-26 | 2011-10-13 | Akkii Foods:Kk | Method for collecting extract liquid containing fucoidan from alga body of brown algae sargassum horneri |
| CN102372790A (en) * | 2011-10-28 | 2012-03-14 | 浙江中烟工业有限责任公司 | Phycophyta polysaccharide as well as preparation method and application thereof to cigarettes |
| CN102488700A (en) * | 2011-12-23 | 2012-06-13 | 上海海洋大学 | Application of sargassan to preparation of medicament for preventing and treating urinary calculus |
| CN102633894A (en) * | 2012-04-16 | 2012-08-15 | 中国科学院宁波材料技术与工程研究所 | Method for preparing high-concentration large-sized seaweed biomass solution and polysaccharide extract |
-
2013
- 2013-12-10 CN CN201310666295.1A patent/CN103739730A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3930509B2 (en) * | 2004-12-21 | 2007-06-13 | 定良 秋月 | A method for collecting a viscous extract containing fucoidan from the brown alga Akamok (scientific name: Sargassumhorneri). |
| JP2011202080A (en) * | 2010-03-26 | 2011-10-13 | Akkii Foods:Kk | Method for collecting extract liquid containing fucoidan from alga body of brown algae sargassum horneri |
| CN102372790A (en) * | 2011-10-28 | 2012-03-14 | 浙江中烟工业有限责任公司 | Phycophyta polysaccharide as well as preparation method and application thereof to cigarettes |
| CN102488700A (en) * | 2011-12-23 | 2012-06-13 | 上海海洋大学 | Application of sargassan to preparation of medicament for preventing and treating urinary calculus |
| CN102633894A (en) * | 2012-04-16 | 2012-08-15 | 中国科学院宁波材料技术与工程研究所 | Method for preparing high-concentration large-sized seaweed biomass solution and polysaccharide extract |
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Application publication date: 20140423 |