JP3930509B2 - A method for collecting a viscous extract containing fucoidan from the brown alga Akamok (scientific name: Sargassumhorneri). - Google Patents

Description

The present invention is a simple method for removing a viscous extract containing fucoidan from a brown alga akamoku having a mature genital bed (scientific name: Sargassum horneri, hereinafter referred to as "protoalgae") without destroying the original algae. It is about the method of obtaining in large quantities.

The viscous extract containing fucoidan that can be obtained from the brown alga Akamoku has a strong anti-proliferative action against AIDS virus and herpes simplex virus. Azidothymidine (A-2T), a well-known anti-AIDS drug, is resistant to viruses. Although it has become a problem, it is expected to be used in combination with the active ingredient of akamoku, showing a synergistic proliferative effect on AIDS virus (Toyama Pharmaceutical Univ.).

It has also been shown to inhibit cancer cell growth ((2000) Nagoya Japan Pharmaceutical Association 118th Annual Meeting P86) and is reported to be effective against cancer cells in a clinical medicine report (Aoyama book “Seaweed Fucoidan” Daisuke Tachikawa Medical Expo).

In addition to nutritional supplements, it is possible to use functional foods that have a biological function improving effect, and it is also used as a cosmetic soap raw material. The viscous extract obtained from brown algae akamoku is versatile. Used for.

However, conventional methods for extracting or collecting the contents or contained substances from seaweed are extremely complicated and use methods such as drying, salting, crushing, boiling, compression, or additives, so the alga bodies are destroyed. Thus, the same algal body was never used again as a raw material (for example, see Patent Documents 1, 2, and 3).

In addition, there is a problem that even if the extraction equipment is attached, it becomes expensive due to a complicated method, and the production cost is increased due to corrosion of the production equipment due to the use of acid, salt, etc. (see, for example, Patent Document 4).

As described above, fucoidan extraction from seaweed is complicated and requires a small amount of extraction and is expensive. However, extraction is not performed on an industrial scale, but the extract of brown algae akamoku increases bone mass. It has also been found that the composition is effective as an agent for preventing or improving diabetes (see, for example, Patent Documents 5 and 6).

JP 2000-346552 A JP 11-1437 A JP 2002-220402 A JP 2000-239301 A JP 2003-26597 A JP 2004-217559 A

Conventionally, the technique of collecting contents such as fucoidan from seaweed as described above has been complicated, requiring equipment, time, various methods, and a large amount of money, and is expensive.

The present invention solves such a conventional problem, and has a sticky shape including fucoidan, which has greatly increased the amount of fucoidan collected with respect to the original alga body from a readily available brown alga akamoku by a simple method. It is to provide a method for collecting an extract.

            The present invention pays attention to the strong vitality and fertility of the genital floor of the brown alga Akamok (scientific name Sargssum horneri), and when the genital bed of the brown alga Akamok matures, it is collected and sprayed with hot water to stimulate it. In this method, a viscous extract containing the fucoidan is collected from the floor over several orders.

            That is, the present invention comprises a hot water sprinkling process in which hot water is sprayed on a brown alga akamok (scientific name: Sargassum horneri) having a mature genital floor, an extraction process for exuding fucoidan, and an extract collecting process for collecting with hot water. In the method of collecting a viscous extract containing fucoidan from brown algae akamoku, the hot water sprinkling step that gives a stimulus by spraying hot water, the extraction step that exudes fucoidan, and the extract recovery that is collected together with hot water After recovering the brown alga akamoku that has passed through the process, in the range of 10 hours to 72 hours in a suitable temperature suitable rehabilitation room, while covering with watering or a compress, a heat retaining or curing process that provides rejuvenation or curing and the hot water sprinkling process with hot water A viscous extract containing fucoidan is collected from the brown alga Akamok, which is obtained by repeatedly repeating the steps of extracting and recovering fucoidan several times. It is the law.

  In addition, the brown alga Akamoku, the original alga body, grows as much as 5 to 6 m, and along with the growth, the genital bed is implanted from the lower part to the upper part. The genital bed is similar to other seaweeds. The fucoidan gradually exudes so much that it is not seen. There are two kinds of brown algae, Akamok, which grow in the vicinity of the Japanese archipelago, the Korean peninsula, and the Chinese coast and reach the maturity stage in spring or autumn.

  Convenience that the original algal bodies collected during the collection period when the genital bed matured easily as described above can be frozen and stored without salting or drying, and the efficacy does not change even if the required amount is processed when necessary. There is sex.

  As mentioned above, the viscous extract containing the fucoidan of the brown alga Akamok that can be used for multiple purposes can be obtained in large quantities for the original alga body in a very simple manner, and is provided at a low price. I can do it.

  Embodiments of the present invention will be described below with reference to FIGS. FIG. 1 is a process diagram showing the method of the present invention.

  FIG. 4 is a specimen drawing of the original algal body and is a part of the original algal body 6 shown in FIGS. FIG. 6 is a general view thereof, and FIG. 5 is a schematic view of each part. In the figure, 10 is a leaf body, 11 is a male genital bed, 11-A is a female genital bed, 12 is a bubble, 13 is a stem, and 14 is a branch. FIG. 7 is a full-scale sample diagram showing the size of the genital bed.

Hereinafter, the method of the present invention will be described with reference to FIG.
Original alga body collecting step 101:
The genital floor 11 and 11-A mature brown alga Akamok (scientific name Sargssum horneri), which is the original algal body 6, collected 2 kg from the beach in the habitat and used it.
Cleaning step 102:
Next, the original algae body 6 is washed with seawater to remove sand and narrow substances, and then washed with fresh water to remove salt.

Step 103 for spreading to a wire mesh:
The above-described raw algal bodies 6 are spread on the wire mesh 5 to an average thickness of about 5 cm using the apparatus shown in FIG.

Hot water sprinkling process 104:
Next, in the hot water sprinkling step 104, hot water is poured into the hot water tank 1, and the hot algae is fed back and forth and left and right from the sprinkler port 3 through the universal pipe 2 which can be moved back and forth and left and right. Sprinkle water into 6. In this case, when the temperature of the hot water is low, there is little irritation to the original algal bodies, and the amount of the extract that has a viscous appearance containing fucoidan is small. As a range of the hot water to be used, it is 50-100 degreeC, As a preferable temperature, it is 90 degreeC or more. In the above operation, hot water of 90 ° C. or more was sprayed with 8 liters of 400% per 2 kg of the original algal bodies. The spraying time was within 1 minute. The longer the spraying time, the more likely it is to damage the genital bed 11, 11-A.

Extraction step 105, extract recovery step 106:
According to this work, the genital floors 11 and 11-A are stimulated, and the extractant receiver 7 contains a viscous extract containing fucoidan together with hot water, and the operations of the primary extraction step 105 and the extract recovery step 106 are performed. Completed.

  The amount of hot water for the original algal body 6 is not necessarily limited to 400%, and depends on the implantation or maturity of the genital beds 11, 11-A. The fucoidan of the brown alga Akamok oozes mainly depending on the genital bed 11, 11-A. As shown in FIG. 4 or 7, the genital bed 11, 11-A is particularly prominent and large in the seaweed. The extraction operation depends on the implantation or maturity. In addition, even if the same extraction operation | work is performed to the original algal body 6 which removed the genital organs 11 and 11-A from the original algal body 6 shown in FIG. 4, the sticky extract containing fucoidan is hardly collected.

The properties of the viscous extract containing fucoidan that could be obtained in this way were as follows.
With B-type viscometer V P M (rotational speed) 60 30 16 12
CP (viscosity) 6 4.3 5.5 7
PH 5.7
Collected amount 8.130cc
The weight of the original algal body 6 after the completion of the primary extraction operation was 1.870 g.

  The stem 13, the leaf body 10, the bubble body 12, and the branch 14 of the original algal body 6 that has undergone the above-mentioned primary extraction work have turned green due to the hot water, but have died, but some of the genital beds 11 and 11 -A are partially There was almost no change except the tip turned black.

Raw alga body recovery process 107, recuperation or curing process 108:
The original algal body 6 was as described above, but more than 70% of the genital organs 11 and 11-A were healthy, so they were taken out of the wire mesh 5 and spread on the original algal body curing container 9 in FIG. It was allowed to cure while covered with water or a compress in the room. In this case, the temperature of the rehabilitation room was 23 ° C., and the humidity was 75%. Although the curing time is while observing the state of the genital bed 11, 11-A, about 10 to 72 hours is appropriate. Reference numeral 8 denotes a shelf frame.

Step 103 for spreading to the secondary wire mesh, secondary water pouring step 104:
The original algal body 6 after 48 hours of curing has exuded viscose fucoidan naturally due to the activity of the genital beds 11, 11-A, and the whole has become sticky. Went.
In this case, considering that it was a secondary collection operation, hot water was 90 ° C. or higher, and 300% of 6 liters of hot alga body 6 was sprinkled. Sprinkling time was less than 1 minute, the same as last time. The weight of the original algal body 6 before this operation was swollen to 2.050 g.

Secondary extraction step 105, secondary extract recovery step 106:
The properties of the viscous extract containing fucoidan collected by performing the secondary collection operation described above were as follows.
With B-type viscometer V P M (rotational speed) 60 30 16 12
CP (viscosity) 5 4 3 6
PH 5.7
Amount collected 6.120cc

Step 103 that extends to the third wire mesh, third water sprinkling step 104, third extraction step 105, third extraction liquid recovery step 106, third raw alga body recovery step 107, third rehabilitation / curing step 8:
The weight of the original algal body 6 after the secondary extraction operation was 1.930 g, and was cured in the rehabilitation room for 24 hours in the same procedure as the previous time. The original algal body 6 after 24 hours of curing exuded a viscous fucoidan from the genital organs beds 11 and 11-A and became sticky throughout, so the third collection operation was performed in the same procedure as the previous time. The weight of the original algal body 6 before the start of the third work was 2.130 g depending on the care of watering and the like. In this case, 6 liters of hot water having a temperature of 90 ° C. or higher was sprayed with an emphasis on the amount from the balance between the viscosity and the amount in consideration of the tertiary work. The properties of the extract that could be obtained were as follows.
With B-type viscometer V P M (rotational speed) 60 30 16 12
CP (viscosity) 1.9 2.1 2.5 4
PH 5.7
Collected amount 6.150cc
The weight of the original algal body 6 after the completion of the third extraction operation was 1.980 g.

Fourth extraction liquid recovery 106, fourth primary algal body recovery step 107, disposal step 109:
The raw algal bodies 6 that have finished the third extraction work are cured in the recuperation room as in the previous time, and after 48 hours, the fourth extraction and collection work are performed in the same procedure, and a viscous form containing fucoidan having the same viscosity as the third work is obtained. 5.1 liters of extract was obtained. In addition, since it was thought that the original algal body 6 became an extract having a low viscosity or a small amount even if the fifth operation was performed, it was finished in the fourth operation and discarded.

  Extraction or collection of a viscous extract containing fucoidan from the brown alga Akamok of the original alga body 6 based on such a method is completely epoch-making. While the algae 6 is cured and the state thereof is observed, the viscosity can be adjusted according to the purpose of use, and a viscous extract containing a large amount of fucoidan can be obtained from the brown alga Akamok.

  The specific configuration of the apparatus and the like used in the method of the present invention is not limited to the above embodiment.

Process drawing which shows embodiment of this invention Front view of the extractor Front view of curing containers and shelf in the same nursing room Protoalgae body specimen used in examples of the present invention Schematic diagram Overall diagram of brown alga akamoku used in the examples of the present invention The same full-scale specimen drawing

Explanation of symbols

DESCRIPTION OF SYMBOLS 101 Original algae body collection process 102 Washing process 103 The process extended to a wire net 104 The pouring process 105 The extraction process 106 The extract collection process 107 The original alga body collection process 108 The recuperation process 109 The disposal process 1 Hot water pot 2 Free pipe
3 Water spout 4 Wire mesh receiving frame 5 Wire mesh
6 Original Algae 7 Extract Receptor 8 Shelf Frame 9 Original Algae Curing Container 10 Leaf 11 Genital Bed (Male)
11-A Genital floor (female)
12 Foam 13 Stem 14 Branch

Claims (1)

Fucoidan from brown algae akamok, which consists of a hot water sprinkling process that sprinkles hot water to stimulate the brown alga akamoku (scientific name Sargassum horneri) with a mature genital floor, an extraction process that exudes fucoidan, and an extract recovery process that collects with hot water. In the method of collecting a viscous extract containing, the brown algae that has passed through the hot water sprinkling step for sprinkling hot water, the extraction step for exuding fucoidan, and the extract recovery step for collecting with hot water After recovering red mock, in the range of 10 to 72 hours in the appropriate temperature and appropriate rehabilitation room, the hot water sprinkling process using hot water and the hot water sprinkling process and the fucoidan extraction and recovery process that provides recuperation or curing while sprinkling or covering with a compress. A method for collecting a viscous extract containing fucoidan from a brown alga Akamok, characterized in that each step of the step is repeated a plurality of times .

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