CN103735939A - Lipid-lowering Chinese medicinal composition and preparation method thereof - Google Patents
Lipid-lowering Chinese medicinal composition and preparation method thereof Download PDFInfo
- Publication number
- CN103735939A CN103735939A CN201310743030.7A CN201310743030A CN103735939A CN 103735939 A CN103735939 A CN 103735939A CN 201310743030 A CN201310743030 A CN 201310743030A CN 103735939 A CN103735939 A CN 103735939A
- Authority
- CN
- China
- Prior art keywords
- parts
- prepared
- active component
- water
- lipid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention discloses a lipid-lowering Chinese medicinal composition and a preparation method thereof. The composition is prepared from the medicinal materials of panaxadiol saponins, Indian buead, oriental waterplantain rhizome, multiflower knotweed tuber, hawthorn, cassia seed, chicken gizzard membrane, malt, Chinese atractylodes, cassia twig and the like serving as raw materials. The invention further provides a preparation method of the lipid-lowering Chinese medicinal composition. By using the composition provided by the invention, the levels (P is less than 0.05) of total cholesterol and triglyceride in mouse and rat serums can be lowered remarkably, and an extremely remarkable lipid-lowering effect is achieved.
Description
Technical field
The present invention relates to pharmaceutical sanitary field, be specifically related to a kind of Chinese traditional medicines depressing lipid composition and method of making the same.
Background technology
Due to lipid metabolism or running extremely make one or more lipids of blood plasma higher than being normally called hyperlipemia, hyperlipemia serious harm health.Hyperlipemia can be divided into constitutional and Secondary cases two classes.Constitutional is with congenital and hereditary relevant, due to single-gene defect or polygenes defect, make to participate in receptor, enzyme or the apolipoprotein caused by abnormal of lipoprotein transhipment and metabolism, or due to environmental factors (diet, nutrition, medicine) with cause by unknown mechanism.Secondary cases is multiple is born in metabolic disorders (diabetes, hypertension, myxedema, hypothyroidism, obesity, hepatorenal disease, adrenal cortex function are hyperfunction), or with other factor ages, sex, season, drink, smoking, diet, physical exertion, psychentonia, emotional activity etc. is relevant.
To be mainly lipid deposit caused xanthoma and lipid deposits caused arteriosclerosis at blood vessel endothelium in intradermal in the clinical manifestation of hyperlipemia.Although hyperlipemia can cause xanthoma, its incidence rate is also not bery high; And atherosclerotic generation and development are a kind of slowly progressive processes.Therefore most of patients non-evident sympton and abnormal sign under normal conditions.Many people just find that there is blood plasma lipoprotein level while carrying out blood biochemical check due to other reasons to raise.
Hyperlipemia is controlled and need to be carried out for a long time, somewhat expensive used, at present Western medicine used is mainly the various diseases that treatment hyperlipemia causes clinically, the diseases such as atherosclerosis, coronary heart disease, pancreatitis, can only control from surface expression, and do not reach the object of radical cure, in addition, Western medicine class has certain Liver and kidney toxicity mostly.
Theory of Chinese medical science thinks, hyperlipidemia is mainly because surfeit, and weakness of the spleen and stomach, stomach digesting food is ineffective, and dysfunction of the spleen in transportation is contained in phlegm-damp and is caused, and is levying of deficiency in origin and excess in superficiality.
Summary of the invention
The object of this invention is to provide a kind of Chinese traditional medicines depressing lipid compositions;
Another object of the present invention is to provide a kind of Chinese traditional medicines depressing lipid preparation method of composition.
The object of the invention is to realize by following technical scheme:
Chinese traditional medicines depressing lipid compositions of the present invention is to be prepared from by the raw material containing following weight portion:
Panoxadiol's glycosides 5-15 part, Poria 30-60 part, Rhizoma Alismatis 30-60 part, Radix Polygoni Multiflori 20-40 part, Fructus Crataegi 20-40 part, Semen Cassiae 10-30 part, Endothelium Corneum Gigeriae Galli 10-30 part, Fructus Hordei Germinatus 10-30 part, Rhizoma Atractylodis 10-30 part, Ramulus Cinnamomi 10-30 part.
Preferably, be to be prepared from by the raw material containing following weight portion:
Panoxadiol's glycosides 8-12 part, Poria 40-50 part, Rhizoma Alismatis 40-50 part, Radix Polygoni Multiflori 25-35 part, Fructus Crataegi 25-35 part, Semen Cassiae 15-25 part, Endothelium Corneum Gigeriae Galli 15-25 part, Fructus Hordei Germinatus 15-25 part, Rhizoma Atractylodis 15-25 part, Ramulus Cinnamomi 15-25 part.
More preferably, be to be prepared from by the raw material containing following weight portion:
10 parts of panoxadiol's glycosides, 45 parts, Poria, 45 parts of Rhizoma Alismatis, 30 parts of the Radixs Polygoni Multiflori, 30 parts of Fructus Crataegis, 20 parts of Semen Cassiaes, 20 parts of Endothelium Corneum Gigeriae Galli, 20 parts, Fructus Hordei Germinatus, 20 parts of Rhizoma Atractylodis, 20 parts of Ramulus Cinnamomi.
Below crude drug source of the present invention:
Panaxadiol saponin: be a kind of of ginsenoside.Ginsenoside (Ginsenoside) is a kind of steroid compound, triterpene saponin.Mainly be present in Araliaceae Panax medical material, Panax's majority is some rare Chinese medicines, as Radix Notoginseng, Radix Ginseng, Radix Panacis Quinquefolii etc.Ginsenoside can be divided into three groups of two classes according to the difference of sapogenin, and a class is the Dammar methane series saponin of tetracyclic triterpene, and wherein one group of acid hydrolysis end product is panoxadiol, as ginsenoside Rb1, Rb2, Rb3, Rc, Rd, Rg3, Rh2 etc.
Poria: this product is On Polyporaceae Poria Poria cocos(Schw.) dry sclerotia of Wolf.More than 7～JIUYUE, excavate, after digging out, remove silt, bank up after " diaphoresis ", spread out and dry in the air to dry tack free, then " diaphoresis ", after extremely now wrinkle, the large portion of internal moisture scatter and disappear for several times repeatedly, dry in the shade, be called " Poria "; Or fresh Poria is pressed to different parts cutting, and dry in the shade, be called " Cortex Sclerotii Poriae " and " Poria piece ".[nature and flavor] are sweet, light, flat.[return through] GUIXIN, lung, spleen, kidney channel.[function cures mainly] promoting diuresis to eliminate damp pathogen, spleen invigorating mind calming.For edema oliguria, phlegm retention vertigo and palpitation, insufficiency of the spleen lack of appetite, have loose bowels in loose stool, irritability, palpitation with fear insomnia.
Rhizoma Alismatis: this product is Notes On Alism At Aceae Rhizoma Alismatis Alisma orientalis(Sam.) dry tuber of Juzep..Excavate when stem and leaf starts to wither winter, and cleaning, drying, remove fibrous root and rough bark.[nature and flavor] are sweet, cold.[return through] returns kidney, urinary bladder channel.[function cures mainly] diuresis, clearing away damp-heat.For dysuria, edema distension, the oliguria of having loose bowels, dizziness due to fluid-retention, the puckery pain of pyretic stranguria; Hyperlipidemia.
The Radix Polygoni Multiflori: this product is the dried root of polygonum multiflorum thunb Polygonum multiflorum Thunb., and its rattan claims " Caulis Polygoni Multiflori ".Autumn, two season of winter excavate when leaf is withered, and the two ends of pruning are cleaned, and the individual large piece that is cut into is dry.[nature and flavor] are bitter, sweet, puckery, temperature.[return through] returns liver, the heart, kidney channel.[function cures mainly] removing toxic substances, eliminating carbuncle, loosening bowel to relieve constipation.For scrofula carbuncle sore, rubella pruritus, dryness of the intestine constipation; Hyperlipidemia.
Fructus Crataegi: this product is the dry mature fruit of rosaceous plant Fructus Pyri Pashiae Crataegus pinnatifida Bge.var.major N.E.Br. or Fructus Crataegi Crataegus pinnatifida Bge..Gather during fruit maturation autumn, and section is dry.[nature and flavor] are sour, sweet, tepor.[return through] returns spleen, stomach, Liver Channel.[function cures mainly] promoting digestion and invigorating the stomach, circulation of qi promoting dissipating blood stasis.For meat stagnation, gastral cavilty distension, dysentery stomachache, blood stasis amenorrhea, postpartum stagnation, trusted subordinate's twinge, hernia pain; Hyperlipemia.The effect of Fructus Crataegi (parched to brown) dyspepsia and intestinal stasis relieving strengthens.For meat stagnation, dysentery is not well.
Semen Cassiae: this product is the dry mature seed of leguminous plant Semen Cassiae Cassia obtusifolia L. or little Semen Cassiae Cassia tora L..The mature fruit of gathering autumn, dries, and lays seed, removes impurity.[nature and flavor] are sweet, bitter, salty, are slightly cold.[return through] returns liver, large intestine channel.[function cures mainly] clearing away heat to improve acuity of vision, loosening bowel to relieve constipation.For the puckery pain of conjunctival congestion, the many tear of photophobia, has a headache dizzy, poor vision, constipation.
Endothelium Corneum Gigeriae Galli: this product is the dry inner wall of sandbag of the chicken Gallus gallus domesticus Brisson of Phasianidae animal man.Kill after chicken, take out chicken gizzard, peel immediately while hot inwall (first do not wash with water, otherwise difficulty being peeled off and easily fragmentation), cleaning, drying.[nature and flavor] are sweet, flat.[return through] returns spleen, stomach, small intestinal, urinary bladder channel.[function cures mainly] invigorating the stomach and promoting digestion, arresting seminal emission.For food stagnation, do not disappear, vomiting dysentery, infantile malnutrition, the enuresis, seminal emission.
Fructus Hordei Germinatus: this product is that the mature fruit of grass Fructus Hordei Vulgaris Hordeum vulgare L. is dried and obtains through germinateing.After wheat grain is soaked in water, keep suitable temperature, humidity, when plumelet grows to about 0.5cm, dry or cold drying.[nature and flavor] are sweet, flat.[return through] returns spleen, stomach warp.[function cures mainly] promote qi circulation digestion promoting, spleen benefiting and stimulating the appetite, moves back newborn relieving distension.For food stagnation, do not disappear, abdominal distention, insufficiency of the spleen lack of appetite, milk smoulders, distending pain of the breast, women's ablactation.
Rhizoma Atractylodis: this product is feverfew Atractylodes lancea (Thunb.) DC. Atractylodes lancea(Thunb.) DC. or Atractylis chinensis Atractylodes chinensis(DC.) dry rhizome of Koidz..Spring, Qiu Erji excavate, and remove silt, dry, and hit fibrous root.[nature and flavor] are pungent, bitter, temperature.[return through] returns spleen, stomach, Liver Channel.[function cures mainly] is drying damp and strengthening spleen, expelling wind and cold, improving eyesight.For distension and fullness in the abdomen, have loose bowels, edema, beriberi flaccidity of feet with lamenness, rheumatic arthralgia, anemofrigid cold, nyctalopia.
Ramulus Cinnamomi: this product is the dry twig of canella Cortex Cinnamomi Cinnamomum cassia Presl.Spring, Xia Erji gather, and except defoliation, dry, or section are dried.[nature and flavor] acrid, sweet, warm.[return through] GUIXIN, lung, urinary bladder channel.[function cures mainly] diaphoresis expelling pathogenic factors from muscles, promoting the flow of QI-blood by warming the meridian, supporing yang activating QI, the flat gas that spins.For anemofrigid cold, coldness and pain in the epigastrium, cold in blood amenorrhea, arthralgia, phlegm retention, edema, cardiopalmus, renal mass.
Another aspect of the present invention has been to provide the preparation method of the active component of Chinese medicine composition of the present invention, and the method is the ethanol extraction that adopts water extraction or 40-80% concentration expressed in percentage by volume.Concrete preparation method is as follows:
Scheme one: get whole medical materials, extracting in water 2-3 time, each amount of water is equivalent to 6-12 times of medical material gross weight, and each extraction time is 1-3 hour, and merge extractive liquid, filters, and filtrate is condensed into fluid extract, obtains described active component.
Scheme two: get whole medical materials, extracting in water 2-3 time, each amount of water is equivalent to 6-12 times of medical material gross weight, each extraction time is 1-3 hour, merge extractive liquid,, filters the clear paste that when filtrate is concentrated into 70-80 ℃, relative density is 1.05-1.20, add ethanol, making containing alcohol amount is 40-70%, and standing 12-24 hour filters, filtrate is condensed into fluid extract, obtains described active component.
Scheme three: get whole medical materials, use 40-80% alcohol reflux 2-3 time, doubly, extraction time is 1-3 hour to the 4-10 that each ethanol consumption is medical material total amount, and merge extractive liquid,, filters, and filtrate is condensed into fluid extract, obtains described active component.
Chinese traditional medicines depressing lipid compositions of the present invention, can coordinate with pharmaceutically acceptable carrier, makes various common dosage forms, as tablet, granule, capsule, syrup, mixture etc.
Pharmaceutically acceptable carrier of the present invention includes but not limited to following:
Diluent: starch, Icing Sugar, lactose, dextrin, microcrystalline Cellulose, inorganic salt, sugar alcohols etc.
Wetting agent and binding agent: purified water, ethanol, gelatin, Polyethylene Glycol, cellulose derivative etc.
Disintegrating agent: starch, carboxymethyl starch sodium, cellulose derivative, polyvinylpolypyrrolidone etc.
Lubricant: magnesium stearate, micropowder silica gel, Pulvis Talci, Polyethylene Glycol etc.
Cosolvent: water, ethanol, glycerol, propylene glycol, liquid Paraffin, plant wet goods.
Correctives: sucrose, monosaccharide, aromatic etc.
Antiseptic: benzoic acid, sorbic acid, methyl ester, ethyl ester, propyl ester etc.
Method 1: tablet
Get scheme one to one of scheme three gained active component, be dried to dried cream powder, add tablet to commonly use adjuvant, production method is prepared into tablet of the present invention routinely.
The conventional adjuvant of above-mentioned tablet comprises one of diluent, wetting agent, binding agent, disintegrating agent, lubricant or whole.
Method 2: granule
Get scheme one to one of scheme three gained active component, be dried to dried cream powder, add granule to commonly use adjuvant, production method is prepared into granule of the present invention routinely.
The conventional adjuvant of above-mentioned granule comprises one of diluent, wetting agent, binding agent, disintegrating agent or whole.
Method 3: capsule
Get scheme one to one of scheme three gained active component, be dried to dried cream powder, add capsule to commonly use adjuvant, production method is prepared into capsule of the present invention routinely.
The conventional adjuvant of above-mentioned capsule comprises one of diluent, wetting agent, binding agent, disintegrating agent, lubricant or whole.
Method 4: syrup
Get scheme one to one of scheme three gained active component, add syrup to commonly use adjuvant, production method is prepared into syrup of the present invention routinely.
The conventional adjuvant of above-mentioned syrup comprises one of correctives, antiseptic, cosolvent or whole.
Method 5: mixture
Get scheme one to one of scheme three gained active component, add mixture to commonly use adjuvant, production method is prepared into mixture of the present invention routinely.
The conventional adjuvant of above-mentioned mixture comprises one of correctives, antiseptic or whole.
Side separates: we's card, by eating and drinking without temperance, is had a liking for food delicious food, causes weakness of the spleen and stomach, disorder in ascending and descending, and failure of the spleen to transport and transform, indigestion and cereal nutrient dysfunction of the spleen in transportation and transformation is made raw phlegm-damp, contains in phlegm-damp to hand over and hinders in blood vessels and hyperamization fat raises.Hyperlipidemia is the card for deficiency in origin and excess in superficiality, should take strengthening vital QI to eliminate pathogenic factors as the rule for the treatment of, controls when dampness removing spleen invigorating, eliminates the phlegm and helps digestion.We be take Poria as monarch drug, Poria is kind ooze sluice wet, make wet without gather, the expectorant life of having no way of, the effect of more double spleen invigorating, two arrogate to oneself its merit, are to take stopgap measures to consolidate.Rhizoma Alismatis, Rhizoma Atractylodis, Ramulus Cinnamomi, can help the heresy of the capable water damp-phlegm of Poria drink, the gas of invigorating the spleen and stomach; Fructus Crataegi, Endothelium Corneum Gigeriae Galli, Fructus Hordei Germinatus help transporting and transforming function of the spleen and stomach water paddy, and six medicines are all ministerial drug.The Radix Polygoni Multiflori, Semen Cassiae are adjuvant drug, with digestants mutual reinforcement between be use, loosening bowel to relieve constipation, benefits the strong fortune of taste.All medicines share, and invigorating the spleen and stomach, to consolidate, eliminates the phlegm and helps digestion to take stopgap measures.
The present invention adds panoxadiol's glycosides (R in above-mentioned side
bsaponins).Panoxadiol's glycosides can promote intact animal's lipid metabolism, reduces blood cholesterol, and has antioxidation, can prevention of arterial atherosis.R wherein
b1can also reduce liver cholesterol, rising HMG-CoA reductase activity; R
b2can improve blood fat and atherogenic index.Above-mentioned Jiangzhi Recipe and panoxadiol's glycosides are collaborative, have the health care of better assistant lipid-lowering.
Technical solution of the present invention meets the mechanism of tcm treatment according to syndrome differentiation, bases oneself upon the origin of disease, reasonable recipe science.
A kind of Chinese traditional medicines depressing lipid compositions provided by the invention has the following advantages:
1, Chinese traditional medicines depressing lipid compositions provided by the invention is safe and effective, compares with hyperlipidemia model group, and the present invention has obviously reduced TC in rat blood serum, TG, HDL, LDL level (P < 0.05), and effect for reducing fat is obvious; Compare with comparative example, Serum Lipids in Experimental HypercholesterolemicRats of the present invention is all significantly lower than comparative example Zu ﹙ P < 0.05 ﹚.
2, in the test of mice hyperlipidemia model, the present invention compares with hyperlipidemia model group, and blood lipid level obviously reduces, and difference is (P < 0.01) very significantly; And compare with comparative example group, blood lipid level of the present invention reduces significant difference (P < 0.05, P < 0.01).
3, the present invention has dampness removing spleen invigorating, the effect of eliminating the phlegm and helping digestion, and treating both the principal and the secondary aspects of a disease at the same time, reaches the object of blood fat reducing.And production cost is low, technique is simple.
The specific embodiment
Below by embodiment, further illustrate the present invention.It should be understood that embodiments of the invention are for the present invention rather than limitation of the present invention are described.The simple modifications that essence according to the present invention is carried out the present invention all belongs to the scope of protection of present invention.
Embodiment 1 syrup
Get panoxadiol's glycosides 10g, Poria 45g, Rhizoma Alismatis 45g, Radix Polygoni Multiflori 30g, Fructus Crataegi 30g, Semen Cassiae 20g, Endothelium Corneum Gigeriae Galli 20g, Fructus Hordei Germinatus 20g, Rhizoma Atractylodis 20g, Ramulus Cinnamomi 20g.By above-mentioned whole medical materials, extracting in water 2 times, twice amount of water is respectively 10 times, 8 times of medical material gross weight, and the extracted twice time is respectively 2 hours, 1 hour, and merge extractive liquid, filters, and filtrate is condensed into fluid extract.In extractum, add syrup adjuvant sucrose, Mel, potassium sorbate, water to stir, make syrup.
Embodiment 2 tablets
Get panoxadiol's glycosides 8g, Poria 40g, Rhizoma Alismatis 40g, Radix Polygoni Multiflori 25g, Fructus Crataegi 25g, Semen Cassiae 15g, Endothelium Corneum Gigeriae Galli 15g, Fructus Hordei Germinatus 15g, Rhizoma Atractylodis 15g, Ramulus Cinnamomi 15g.By above-mentioned whole medical materials, with 40% alcohol reflux 3 times, each ethanol consumption is respectively 10 times, 8 times, 4 times of medical material total amount, 3 hours, 2 hours, 1 hour respectively extraction time, merge extractive liquid,, filters, filtrate is condensed into after fluid extract, be dried to dried cream powder, add supplementary product starch, ethanol, Pulvis Talci, make tablet.
Embodiment 3 mixture
Get panoxadiol's glycosides 12g, Poria 50g, Rhizoma Alismatis 50g, Radix Polygoni Multiflori 35g, Fructus Crataegi 35g, Semen Cassiae 25g, Endothelium Corneum Gigeriae Galli 25g, Fructus Hordei Germinatus 25g, Rhizoma Atractylodis 25g, Ramulus Cinnamomi 25g.By above-mentioned whole medical materials, extracting in water 3 times, three amount of water are equivalent to respectively 12 times, 8 times, 6 times of medical material gross weight, 3 hours, 2 hours, 1 hour respectively each extraction time, merge extractive liquid,, filters, the clear paste that when filtrate is concentrated into 70 ℃, relative density is 1.20, adds ethanol, and making containing alcohol amount is 40%, standing 24 hours, filter, filtrate is condensed into fluid extract, and adding purified water ad pond om is 3 times of medical material gross weight, fill, obtains mixture of the present invention.
Add mixture adjuvant sucrose, ethyl ester, water, potassium sorbate to stir, make mixture.
Embodiment 4 tablets
Get panoxadiol's glycosides 5g, Poria 30g, Rhizoma Alismatis 30g, Radix Polygoni Multiflori 20g, Fructus Crataegi 20g, Semen Cassiae 10g, Endothelium Corneum Gigeriae Galli 10g, Fructus Hordei Germinatus 10g, Rhizoma Atractylodis 10g, Ramulus Cinnamomi 10g.By above-mentioned whole medical materials alcohol reflux 2 times, each concentration of alcohol is respectively 80%, 60%, each ethanol consumption is respectively 10 times, 8 times of medical material total amount, extraction time is respectively 4 hours, 3 hours, and merge extractive liquid, filters, filtrate is condensed into after fluid extract, be dried to dried cream powder, add microcrystalline Cellulose, cross-linking sodium carboxymethyl cellulose, micropowder silica gel, make tablet.
Embodiment 5 capsules
Get panoxadiol's glycosides 15g, Poria 60g, Rhizoma Alismatis 60g, Radix Polygoni Multiflori 40g, Fructus Crataegi 40g, Semen Cassiae 30g, Endothelium Corneum Gigeriae Galli 30g, Fructus Hordei Germinatus 30g, Rhizoma Atractylodis 30g, Ramulus Cinnamomi 30g.By above-mentioned whole medical materials, extracting in water 3 times, three amount of water are respectively 12 times, 8 times, 6 times of medical material gross weight, three extraction times are respectively 3 hours, 2 hours, 1 hour, and merge extractive liquid, filters, filtrate is condensed into after fluid extract, be dried to dried cream powder, add supplementary product starch, ethanol, dextrin, magnesium stearate, incapsulate shell, make capsule.
Embodiment 6 granules
Get panoxadiol's glycosides 10g, Poria 60g, Rhizoma Alismatis 30g, Radix Polygoni Multiflori 25g, Fructus Crataegi 35g, Semen Cassiae 20g, Endothelium Corneum Gigeriae Galli 10g, Fructus Hordei Germinatus 30g, Rhizoma Atractylodis 20g, Ramulus Cinnamomi 10g.By above-mentioned whole medical materials, extracting in water 2 times, each amount of water is equivalent to respectively 12 times, 10 times of medical material gross weight, each extraction time is respectively 3 hours, 2 hours, and merge extractive liquid, filters, filtrate is concentrated into the clear paste that at 80 ℃, relative density is 1.05, adds ethanol, and making containing alcohol amount is 70%, standing 12 hours, filter, filtrate is condensed into after fluid extract, is dried to dried cream powder, add adjuvant Icing Sugar, starch, dextrin, ethanol, make granule.
Embodiment 7 powder
Take panoxadiol's glycosides 5g, Poria 30g, Rhizoma Alismatis 45g, Radix Polygoni Multiflori 35g, Fructus Crataegi 40g, Semen Cassiae 10g, Endothelium Corneum Gigeriae Galli 15g, Fructus Hordei Germinatus 20g, Rhizoma Atractylodis 10g, Ramulus Cinnamomi 30g.Medical material cleans, pulverizes, and crosses 80-100 mesh sieve, obtains powder of the present invention.
Pharmacodynamics test: fat-reducing effect evaluation
One, the impact on effect for reducing fat
1 experiment material
1.1 animal
SD rat, male, body weight 180～210g, buys from Guangxi Medical University's Experimental Animal Center (production licence number: SCXK osmanthus 2009-0002).
1.2 medicines and instrument
Embodiment of the present invention 1-5, lotus essence procyanidin capsule (lotus essence biological product Science and Technology Ltd.), automatic clinical chemistry analyzer (manufacturer: step auspicious medical international corporation, model: BS-190).
2 experiment groupings
Select 80 of healthy SD rats, male, be divided at random 8 groups, 10 every group:
Blank group: gavage equal-volume distilled water;
Hyperlipidemia model group: gavage equal-volume distilled water;
Experimental group: give embodiment of the present invention 1-5 sample liquid;
Contrast groups: give lotus essence procyanidin sample liquid.
3 experimental techniques
The 3.1 modeling phases: rat is divided into blank group (10) at random, all the other are high fat group, two groups of rat body weight there was no significant difference (t=0.1132, P > 0.05), blank group gives normal diet, and high fat group gives high lipid food (79% normal feedstuff+1% cholesterol+10% yolk powder+10% Adeps Sus domestica).After 4 weeks, blank group and high fat group are randomly drawed 10 animals and are got eye socket venous blood mensuration serum TC, TG, HDL, LDL, and fat group as high in testing result is significantly higher than blank group, and rat hyperlipidemia model forms.
3.2 experimental period: 70 rats of the high lipid food of feeding are divided at random: model group, embodiment 1-5 group and comparative example group, every group of 10 animals.The body weight difference not statistically significant of 7 groups of rats (F=0.2681, P > 0.05).After experiment, within 5 weeks, be experimental period, except blank group, all the other 7 treated animals continue the high lipid food of feeding, and embodiment and comparative example group are pressed scheme gavage every day, model group and the isopyknic distilled water of blank group gavage, and gavage volume is 10ml/kg.
When experiment finishes, water 12h is can't help in rat fasting.Broken end is got blood, surveys the content of serum lipids.
4 experimental results
The blank group of table 1 and high fat group rat modeling phase lipids detection result
Note: with the comparison of blank group, * P < 0.05, * * P < 0.01.
Table 1 result shows: the TC of high fat group, TG, HDL, LDL value are all higher than blank group, and significant difference (P < 0.05), illustrates rat hyperlipidemia model modeling success.
The impact of each tested material of table 2 on rat fat
Note: with the comparison of blank group,
▲ ▲p < 0.01; With model control group comparison, * P < 0.05, * * P < 0.01; With the comparison of comparative example group,
#p < 0.05,
##p < 0.01.
Table 2 result shows: compare with hyperlipidemia model group, embodiment of the present invention 1-5, comparative example have all obviously reduced TC in rat blood serum, TG, HDL, LDL level (P < 0.05), and effect for reducing fat is obvious; Compare with comparative example, embodiment 1-3 group Serum Lipids in Experimental HypercholesterolemicRats is all significantly lower than comparative example Zu ﹙ P < 0.05 ﹚.
Two, the impact on mice effect for reducing fat
1 experiment material
1.1 animal
SPF level Kunming kind white mice, robust half and half, body weight 20 ± 2g, buys from Guangxi Medical University's Experimental Animal Center (production licence number: SCXK osmanthus 2009-0002).
1.2 medicines and instrument
Embodiment of the present invention 1-5,0.9% normal saline (Pharmaceutical Group Co.,Ltd of Nanning Baihui), lotus essence procyanidin capsule (lotus essence biological product Science and Technology Ltd.), automatic clinical chemistry analyzer (manufacturer: step auspicious medical international corporation, model: BS-190).
2 experiment groupings
Select 80 of healthy mices, male and female half and half, are divided into 8 groups at random, 10 every group:
Blank group: gavage equal-volume distilled water;
Hyperlipidemia model group: gavage equal-volume distilled water;
Experimental group: give embodiment 1-5 sample liquid of the present invention;
Contrast groups: give lotus essence procyanidin sample liquid.
3 experimental techniques
Each is organized continuous gavage and gives tested material 11d, and gavage volume is 0.2ml/10g, and 10d is except blank group in experiment, and all the other are respectively organized lumbar injection 75% egg yolk Emulsion (75% egg yolk normal saline suspension) 0.1mL/10g and carry out modeling.Before last gavage, water 12h is can't help in animal fasting, and after last gavage, 1h mice is plucked eyeball and gets blood, and the centrifugal 4min separation of serum of 3000r/min is measured TC, TG value.
4 experimental results
The impact of each tested material of table 3 on hyperlipidemia model lipid of mice level due to Ovum Gallus domesticus album Emulsion
Note: with the comparison of blank group,
▲ ▲p < 0.01; With model control group comparison, * P < 0.05, * * P < 0.01; With the comparison of comparative example group,
#p < 0.05,
##p < 0.01.
Table 3 result shows: hyperlipidemia model group serum total cholesterol and triglyceride be higher than blank group, and difference very significantly (P < 0.01); Embodiment 1-5 group, comparative example group are compared with hyperlipidemia model group, and blood lipid level obviously reduces, and difference is (P < 0.01) very significantly; Compare with comparative example group, embodiment 1-5 group blood lipid level reduces significant difference (P < 0.05, P < 0.01).Result shows, embodiment of the present invention 1-5 has lipid-reducing function.
Conclusion: with the comparison of hyperlipidemia model group, the present invention can obviously reduce T-CHOL and triglyceride levels (P < 0.05) in mice, rat blood serum, and effect for reducing fat is extremely remarkable; Compare with comparative example, give mice of the present invention, Serum Lipids in Experimental HypercholesterolemicRats all significantly lower than comparative example Zu ﹙ P < 0.05 ﹚, point out lipid-lowering effect of the present invention better.
Although, above used general explanation, the specific embodiment and test, the present invention is described in detail, on basis of the present invention, can make some modifications or improvements it, and this will be apparent to those skilled in the art.Therefore, these modifications or improvements, all belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (8)
1. a Chinese traditional medicines depressing lipid compositions, is characterized in that it is to be prepared from by the raw material that comprises following weight portion: panoxadiol's glycosides 5-15 part, Poria 30-60 part, Rhizoma Alismatis 30-60 part, Radix Polygoni Multiflori 20-40 part, Fructus Crataegi 20-40 part, Semen Cassiae 10-30 part, Endothelium Corneum Gigeriae Galli 10-30 part, Fructus Hordei Germinatus 10-30 part, Rhizoma Atractylodis 10-30 part, Ramulus Cinnamomi 10-30 part.
2. Chinese traditional medicines depressing lipid compositions as claimed in claim 1, is characterized in that it is to be prepared from by the raw material that comprises following weight portion: panoxadiol's glycosides 8-12 part, Poria 40-50 part, Rhizoma Alismatis 40-50 part, Radix Polygoni Multiflori 25-35 part, Fructus Crataegi 25-35 part, Semen Cassiae 15-25 part, Endothelium Corneum Gigeriae Galli 15-25 part, Fructus Hordei Germinatus 15-25 part, Rhizoma Atractylodis 15-25 part, Ramulus Cinnamomi 15-25 part.
3. Chinese traditional medicines depressing lipid compositions as claimed in claim 1, is characterized in that it is to be prepared from by the raw material that comprises following weight portion: 10 parts of panoxadiol's glycosides, 45 parts, Poria, 45 parts of Rhizoma Alismatis, 30 parts of the Radixs Polygoni Multiflori, 30 parts of Fructus Crataegis, 20 parts of Semen Cassiaes, 20 parts of Endothelium Corneum Gigeriae Galli, 20 parts, Fructus Hordei Germinatus, 20 parts of Rhizoma Atractylodis, 20 parts of Ramulus Cinnamomi.
4. the Chinese medicine composition as described in claim 1-3 any one, the active component that it is characterized in that it is to adopt the ethanol extraction of water extraction or 40-80% concentration expressed in percentage by volume to be prepared from.
5. Chinese medicine composition as claimed in claim 4, the active component that it is characterized in that it is prepared from by the following method: get whole medical materials, extracting in water 2-3 time, each amount of water is equivalent to 6-12 times of medical material gross weight, each extraction time is 1-3 hour, and merge extractive liquid, filters, filtrate is condensed into fluid extract, obtains described active component.
6. Chinese medicine composition as claimed in claim 4, the active component that it is characterized in that it is prepared from by the following method: get whole medical materials, extracting in water 2-3 time, each amount of water is equivalent to 6-12 times of medical material gross weight, each extraction time is 1-3 hour, merge extractive liquid,, filter, the clear paste that when filtrate is concentrated into 70-80 ℃, relative density is 1.05-1.20, adds ethanol, and making containing alcohol amount is 40-70%, standing 12-24 hour, filter, filtrate is condensed into fluid extract, obtains described active component.
7. Chinese medicine composition as claimed in claim 4, the active component that it is characterized in that it is prepared from by the following method: get whole medical materials, with 40-80% alcohol reflux 2-3 time, the 4-10 that each ethanol consumption is medical material total amount doubly, extraction time is 1-3 hour, and merge extractive liquid, filters, filtrate is condensed into fluid extract, obtains described active component.
8. a Chinese traditional medicines depressing lipid compositions, comprises acceptable carrier in the Chinese traditional medicines depressing lipid compositions of any one in claim 1-3 and pharmaceuticals industry.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310743030.7A CN103735939A (en) | 2013-12-30 | 2013-12-30 | Lipid-lowering Chinese medicinal composition and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310743030.7A CN103735939A (en) | 2013-12-30 | 2013-12-30 | Lipid-lowering Chinese medicinal composition and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103735939A true CN103735939A (en) | 2014-04-23 |
Family
ID=50493055
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310743030.7A Pending CN103735939A (en) | 2013-12-30 | 2013-12-30 | Lipid-lowering Chinese medicinal composition and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103735939A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104825557A (en) * | 2015-05-20 | 2015-08-12 | 南京华宽信息咨询中心 | Compound lipid-lowering linseed oil soft capsule |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1615896A (en) * | 2003-11-12 | 2005-05-18 | 云南省药物研究所 | Medicine for regulating blood fat and treating cardiocerehral disease and preparing method |
| CN101015668A (en) * | 2007-01-16 | 2007-08-15 | 李卓伦 | Fatty liver treating medicine |
| CN102188560A (en) * | 2010-03-08 | 2011-09-21 | 张锡林 | Traditional Chinese medicine for treating hyperlipidemia |
| CN102657822A (en) * | 2012-05-29 | 2012-09-12 | 牡丹江医学院 | Traditional Chinese medicine for treating fatty liver |
-
2013
- 2013-12-30 CN CN201310743030.7A patent/CN103735939A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1615896A (en) * | 2003-11-12 | 2005-05-18 | 云南省药物研究所 | Medicine for regulating blood fat and treating cardiocerehral disease and preparing method |
| CN101015668A (en) * | 2007-01-16 | 2007-08-15 | 李卓伦 | Fatty liver treating medicine |
| CN102188560A (en) * | 2010-03-08 | 2011-09-21 | 张锡林 | Traditional Chinese medicine for treating hyperlipidemia |
| CN102657822A (en) * | 2012-05-29 | 2012-09-12 | 牡丹江医学院 | Traditional Chinese medicine for treating fatty liver |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104825557A (en) * | 2015-05-20 | 2015-08-12 | 南京华宽信息咨询中心 | Compound lipid-lowering linseed oil soft capsule |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100374538C (en) | Fire internal organ nourishing wine and its preparation technology | |
| CN104435775A (en) | Chinese medicinal health-care spirit and preparation method thereof | |
| CN103298477B (en) | Comprise Lignum Cudraniae tricuspidatae and Semen Coicis for suppressing fat or falling hypoglycemic composition and use thereof | |
| CN108524814A (en) | A kind of Chinese medicine composition and preparation method thereof for reducing blood glucose | |
| CN112336815A (en) | Anti-aging composition for strengthening body, preventing cancer and conditioning hypertension, hyperglycemia and hyperlipidemia and application thereof | |
| CN104757669B (en) | A kind of hypertension and hyperlipemia health beverage and preparation method thereof | |
| CN103749818B (en) | A kind of sealwort hypoglycemic health protection tea and preparation method thereof | |
| CN105124078A (en) | Gardenia blood-glucose-reducing and lipid-reducing healthcare tea and preparing method thereof | |
| CN107519445A (en) | A kind of spacetabs type chronic gastritis Chinese medicinal granule and preparation method thereof | |
| CN106177053A (en) | A kind of Hyperglycemic health care compositions comprising leaf of Cyclocarya paliurus Iljinskaja and Pericarpium Citri Reticulatae | |
| CN110051815A (en) | A kind of auxiliary hyperglycemic food ball and preparation method thereof | |
| CN110604773B (en) | Composition for regulating lipid metabolism disorder and preparation method and application thereof | |
| CN106177477A (en) | A kind of health composition for blood sugar lowering, blood fat reducing and blood pressure lowering | |
| CN107125751B (en) | Tortoise powder, preparation method and application thereof | |
| CN104957515A (en) | Steamed buns assisting to lower blood sugar and preparation method thereof | |
| CN105533749B (en) | It is a kind of containing Herba Epimedii, the health food of Schisandra chinensis and its preparation method and application | |
| CN110583974A (en) | Slimming whole grain nutritional and healthy meal replacement food and preparation method thereof | |
| CN101703637B (en) | Compound lipidol-reducing extract preparation granule and production technique thereof | |
| CN103735939A (en) | Lipid-lowering Chinese medicinal composition and preparation method thereof | |
| CN107812115A (en) | A kind of Chinese medicine composition for treating diabetes | |
| CN103340411B (en) | Lung moistening oil tea camellia honey | |
| CN104940720A (en) | Traditional Chinese medicine pills for preventing and treating contrast-induced nephropathy and preparation method thereof | |
| CN110314209A (en) | A kind of stomach-nourishing mixture | |
| CN109349513A (en) | A kind of meal replacement powder and application thereof with hypolipemic function | |
| CN114246337B (en) | Composition for strengthening spleen, eliminating dampness and losing weight as well as preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140423 |