CN103734124A - Method for preserving orthopedic transplant material - Google Patents
Method for preserving orthopedic transplant material Download PDFInfo
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- CN103734124A CN103734124A CN201410000005.4A CN201410000005A CN103734124A CN 103734124 A CN103734124 A CN 103734124A CN 201410000005 A CN201410000005 A CN 201410000005A CN 103734124 A CN103734124 A CN 103734124A
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- orthopaedics
- graft materials
- transplant material
- preserving
- iodophor
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Abstract
The invention discloses a method for preserving an orthopedic transplant material. The method comprises the following steps: (1) directly placing the orthopedic transplant material, obtained under sterile conditions, in a vessel containing preservative fluid, hermetically packaging, and then, refrigerating in a refrigerating chamber of an ordinary refrigerator for later use, wherein the preservative fluid contains 1% iodophor; (2) during use, opening a package, and flushing the iodophor on the orthopedic transplant material with normal saline so that the orthopedic transplant material can be used. According to the method, special equipment is not required, the preservation period is long, the maintaining cost is low, the orthopedic transplant material is immediately available, the method is convenient and fast, and meanwhile, the risk of transmitted infection can be avoided, so that the method is easily popularized to medical treatment units of various levels, and the medical treatment cost can be effectively reduced.
Description
Technical field
The present invention relates to a kind of store method of orthopaedics graft materials, particularly relate to the allogeneic of clinical medicine orthopaedics or the xenograft material sterilizing store method under low temperature environment in vitro.
Background technology
In Orthopedic Clinical work, orthopaedics linked groups comprises the tissues such as bone, cartilage, nerve, tendon, ligament, meniscus, can not normal healing after a lot of tissue damages.Must use allogeneic material to transplant repairs or rebuilds.At present, allogeneic material preservation method has freeze-drying, profound hypothermia preservation method or the fresh method of deeply freezing.Wherein cryochem be under freezing conditions tissue water content is down to minimum, then tissue can preserve at normal temperatures.But the material that this method is processed becomes fragile, and mechanical strength obviously lowers.Tendon tissue almost completely loses toughness, is still easy to fracture after rehydration, cannot use.Bone tissue can be used, but after transplanting, new osteanagenesis is very slow.
It is that graft materials is preserved in the profound hypothermia refrigerator of subzero 80 left and right that profound hypothermia is preserved, and extraordinary refrigerator is expensive, and retention cycle is short, and expense is high.
Freshly deeply freeze that to preserve be the tissue that does not pass through sterilization treatment, after aseptic drawing materials, directly put into profound hypothermia Refrigerator store.This method is propagated the risk infecting to be increased, and comprises bacterium, fungi, virus (hepatitis, acquired immune deficiency syndrome (AIDS) etc.).
Organizing that above method is preserved is expensive, such as, freeze drying bone reaches every gram of 500 yuan of left and right, considerably beyond the price of gold.
Summary of the invention
For the keeping method of prior art, there is the shortcoming of complex process, cost costliness, the object of the present invention is to provide a kind of store method of orthopaedics graft materials, the method is without special installation, retention cycle is long, and standing charges are low, immediately available, convenient and swift, can avoid propagating the risk infecting, be easy to, in other medical institutions' popularization of various levels, can effectively reduce medical expense simultaneously.
Realizing object of the present invention can be by taking following technical scheme to reach:
A store method for orthopaedics graft materials, is characterized in that carrying out according to the following steps:
1) the orthopaedics graft materials obtaining under aseptic condition is directly put into and contained the container of preserving liquid, after packing, be put in the refrigerating chamber of general refrigerator and refrigerate, standby; Wherein, preserve that in liquid, to contain concentration be 1% Iodophor;
Described orthopaedics graft materials comprises bone, cartilage, meniscus, tendon, ligament or nerve etc.
2) while using, unpack, with physiological saline, the Iodophor on orthopaedics graft materials is rinsed well, can use.
Realizing object of the present invention can also be by taking following technical scheme to reach:
Realizing one embodiment of the present invention is: in step 1), the temperature of refrigeration is controlled at-4 ℃-0 ℃.
Realizing one embodiment of the present invention is: in step 1), also need in preserving liquid, add dimethyl sulfoxide (DMSO).
Realizing one embodiment of the present invention is: in step 1), the addition of described dimethyl sulfoxide (DMSO) is for preserving the 3%-5% of liquid weight.
Realizing one embodiment of the present invention is: step 2), before use, by sodium thiosulfate, orthopaedics graft materials is taken off to iodine.
Beneficial effect of the present invention is:
The present invention uses common Iodophor liquid, common refrigerator, without special installation, retention cycle is long, can preserve 5 years, standing charges are low, immediately available, convenient and swift, can avoid propagating the risk infecting simultaneously, by national situation of all-level hospitals is had ready conditions, carry out the donation of orthopaedics linked groups, greatly reduce correlative charges.Store method of the present invention, be applicable to operation in excision tissue, the femoral head excising during such as artificial hip joint replacing, the sclerotin excising during knee prosthesis, meniscus and ligamentaum cruciatum, the tissue that limbs are with of amputation.Also can be for preserving the various orthopaedics linked groups of cadaver donor.
Embodiment
Below, in conjunction with embodiment, the present invention is described further:
Embodiment 1:
A store method for orthopaedics graft materials, carries out according to the following steps:
1) the orthopaedics graft materials obtaining under aseptic condition is directly put into and contained the container of preserving liquid, after packing, be put in the refrigerating chamber of general refrigerator and refrigerate, standby; Wherein, preserve that in liquid, to contain concentration be 1% Iodophor; The temperature of refrigeration is controlled at 0 ℃.
2) while using, unpack, with physiological saline, the Iodophor on orthopaedics graft materials is rinsed well, can use.
Embodiment 2:
A store method for orthopaedics graft materials, carries out according to the following steps:
1) the orthopaedics graft materials obtaining under aseptic condition is directly put into and contained the container of preserving liquid, after packing, be put in the refrigerating chamber of general refrigerator and refrigerate, standby; Wherein, preserve that in liquid, to contain concentration be 1% Iodophor; The temperature of refrigeration is controlled at-2 ℃.
2) while using, unpack, with physiological saline, the Iodophor on orthopaedics graft materials is rinsed well, can use.
Embodiment 3:
A store method for orthopaedics graft materials, carries out according to the following steps:
1) the orthopaedics graft materials obtaining under aseptic condition is directly put into and contained the container of preserving liquid, after packing, be put in the refrigerating chamber of general refrigerator and refrigerate, standby; Wherein, preserve that in liquid, to contain concentration be 1% Iodophor; The temperature of refrigeration is controlled at-4 ℃.
2) while using, unpack, with physiological saline, the Iodophor on orthopaedics graft materials is rinsed well, can use.
Embodiment 4:
The feature of the present embodiment is: in step 1), also need in preserving liquid, add dimethyl sulfoxide (DMSO).The addition of described dimethyl sulfoxide (DMSO) is for preserving 3% of liquid weight.Other are identical with specific embodiment 1.
Embodiment 5:
The feature of the present embodiment is: in step 1), also need in preserving liquid, add dimethyl sulfoxide (DMSO).The addition of described dimethyl sulfoxide (DMSO) is for preserving 5% of liquid weight.Other are identical with specific embodiment 1.
Embodiment 6:
The feature of the present embodiment is: step 2), before use, by sodium thiosulfate, orthopaedics graft materials is taken off to iodine.Other are identical with specific embodiment 1.
For a person skilled in the art, can make other various corresponding changes and distortion according to technical scheme described above and design, and these all changes and distortion all should belong to the protection domain of the claims in the present invention within.
Claims (5)
1. a store method for orthopaedics graft materials, is characterized in that carrying out according to the following steps:
1) the orthopaedics graft materials obtaining under aseptic condition is directly put into and contained the container of preserving liquid, after packing, be put in the refrigerating chamber of general refrigerator and refrigerate, standby; Wherein, preserve that in liquid, to contain concentration be 1% Iodophor;
2) while using, unpack, with physiological saline, the Iodophor on orthopaedics graft materials is rinsed well, can use.
2. the store method of orthopaedics graft materials according to claim 1, is characterized in that: in step 1), the temperature of refrigeration is controlled at-4 ℃-0 ℃.
3. the store method of orthopaedics graft materials according to claim 1, is characterized in that: in step 1), also need in preserving liquid, add dimethyl sulfoxide (DMSO).
4. the store method of orthopaedics graft materials according to claim 3, is characterized in that: in step 1), the addition of described dimethyl sulfoxide (DMSO) is for preserving the 3%-5% of liquid weight.
5. according to the store method of the orthopaedics graft materials described in any one in claim 1-4, it is characterized in that: step 2) in, before use, by sodium thiosulfate, orthopaedics graft materials is taken off to iodine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410000005.4A CN103734124B (en) | 2014-01-01 | 2014-01-01 | The store method of a kind of orthopedic transplant material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410000005.4A CN103734124B (en) | 2014-01-01 | 2014-01-01 | The store method of a kind of orthopedic transplant material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103734124A true CN103734124A (en) | 2014-04-23 |
| CN103734124B CN103734124B (en) | 2016-06-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410000005.4A Expired - Fee Related CN103734124B (en) | 2014-01-01 | 2014-01-01 | The store method of a kind of orthopedic transplant material |
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| CN (1) | CN103734124B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112042636A (en) * | 2020-09-02 | 2020-12-08 | 江西美西源再生医学科技有限公司 | Preservation method of autologous skull flap containing rhBMP-2 and provided with periosteum |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1528469A (en) * | 2003-10-09 | 2004-09-15 | 中国人民解放军南京军区南京总医院 | Method for preparing and preserving homogeneous bone tissue with biological activity |
| WO2010021714A2 (en) * | 2008-08-20 | 2010-02-25 | Anthrogenesis Corporation | Improved cell composition and methods of making the same |
-
2014
- 2014-01-01 CN CN201410000005.4A patent/CN103734124B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1528469A (en) * | 2003-10-09 | 2004-09-15 | 中国人民解放军南京军区南京总医院 | Method for preparing and preserving homogeneous bone tissue with biological activity |
| WO2010021714A2 (en) * | 2008-08-20 | 2010-02-25 | Anthrogenesis Corporation | Improved cell composition and methods of making the same |
| WO2010021714A3 (en) * | 2008-08-20 | 2010-04-15 | Anthrogenesis Corporation | Improved cell composition and methods of making the same |
Non-Patent Citations (4)
| Title |
|---|
| 云强 等: "’医用酒精保存自体颅骨治疗颅骨缺损", 《中国民康医学》 * |
| 亓建洪 等: "人骨软骨组织体外冷冻保存的效果", 《中国组织工程研究与临床康复》 * |
| 李媛媛: "用硫代硫酸钠清除碘痕迹", 《中国误诊学杂志》 * |
| 衡雪源 等: "碘伏浸泡冷冻保存自体颅骨行颅骨修补术临床研究", 《济宁医学院学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112042636A (en) * | 2020-09-02 | 2020-12-08 | 江西美西源再生医学科技有限公司 | Preservation method of autologous skull flap containing rhBMP-2 and provided with periosteum |
| CN112042636B (en) * | 2020-09-02 | 2022-05-13 | 江西省元化低温医学科技有限公司 | Preservation method of autologous skull flap containing rhBMP-2 and periosteum |
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|---|---|
| CN103734124B (en) | 2016-06-01 |
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