CN1528469A - Method for preparing and preserving homogeneous bone tissue with biological activity - Google Patents
Method for preparing and preserving homogeneous bone tissue with biological activity Download PDFInfo
- Publication number
- CN1528469A CN1528469A CNA2003101060340A CN200310106034A CN1528469A CN 1528469 A CN1528469 A CN 1528469A CN A2003101060340 A CNA2003101060340 A CN A2003101060340A CN 200310106034 A CN200310106034 A CN 200310106034A CN 1528469 A CN1528469 A CN 1528469A
- Authority
- CN
- China
- Prior art keywords
- bone
- tissue
- osseous tissue
- same race
- preserve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Materials For Medical Uses (AREA)
Abstract
The invention relates to a method to prepare and preserve homogeneous bioactive bone tissue, its steps: 1, obtaining: on the aseptic condition, obtaining homogeneous bone tissue; 2, finishing: eliminating soft tissue and periosteum, eliminating bone marrow tissue, and reserving partial joint bursa, ligament and adhesive point of muscle; 3, defatting: dipping in chloroformic methanol, washing with physiological saline and then packaging; 4, gradient temperature reducing: placing the preprocessed bone tissue in program temperature reducer to make gradient temperature reduction up to -40 deg.C--60 deg.C for 20-80 min.; 5, preserving: putting the bone tissue in the -60 deg.C - 80 deg. C refrigerator. It can keep the integrality of bone ultramicro structure, bone inducing activity, bioforce intensity and maintain a certain quantity of survival soft bone cells.
Description
One, affiliated technical field
The present invention relates to the damaged biomaterial of a kind of repair and reconstruction bone, particularly a kind of preparation and store method that possesses biological activity osseous tissue of the same race.
Two, background technology
Osseous tissue of the same race have the source wide, advantages such as biological healing and medical expense are low are provided, therefore be widely used in osteoarthrosis defect repair that a variety of causes causes and rebuild treatment.American-European countries, 1,000,000 of annual approximately uses, the clinical practice of China's homogeneous bone transplantation were just set up regular osseous tissue tissue bank in nearly 10 years from eighties of last century the fifties, and approximately use 20,000 every year.
The product that present osseous tissue storehouse provides comprises fresh food frozen bone, lyophilization bone and decalcification bone.Preparation process: draw materials, finishing, defat/decalcification, sterilization, freezing and profound hypothermia preserve, its preparation method is directly freezing and cobalt source radiation sterilization, osseous tissue after treatment loses its biological activity, and the biomechanical strength of bone obviously reduces after the cobalt source radiation simultaneously.According to organizing the cold injury theory, if osseous tissue under the room temperature is directly put under-80 ℃ or the more cryogenic environment, the inconsistent of bone top layer and deep layer rate of temperature fall will cause the inconsistent of the interior ice crystal formation of bone and its cells, occur that ice crystal forms in the cell (intracellular ice formation, IIF), the influence of pair cell is the key factor of cell injury in the vitreous degeneration of the cold shock of cell, extracellular fluid and the frozen-thaw process.Cause preparing active disappearance of biotic induce of back osseous tissue of the same race, biomechanical strength descends, and occurs complication such as fracture, nonunion and joint function disturbance after the transplanting, seriously restricts clinical practice.In the frozen research of free cell, freezing biologists find that slowly cooling and rapid rewarming help the survival of cell, carrying out comprising aspect the organ applied basic research of heart, kidney and liver etc. in states such as America and Europes, aspect osseous tissue, do not appearing in the newspapers.
Three, summary of the invention
The object of the present invention is to provide a kind of possess good biotic induce activity and biomechanical strength, keep the chondrocyte of some biologically actives, increase the back symphysis ability of the same race of transplanting, reduce the incidence rate of complication, function of joint after improve transplanting is clinical preparation that possesses biological activity osseous tissue of the same race and the store method that suitable biological implantation material is provided.
The technical solution that realizes the object of the invention is: a kind of preparation and store method that possesses biological activity osseous tissue of the same race the steps include: 1, the obtaining of bioactivity osseous tissue: obtain osteoarthrosis tissue of the same race under aseptic condition; 2, the finishing of bioactivity osseous tissue: reject soft tissue and periosteum, remove myeloid tissue, the attachment point of reserve part joint capsule, ligament and muscle; 3, the defat of bioactivity osseous tissue: chloroform methanol soaks, and packs behind the normal saline flushing; 4, gradient cooling: pretreated osseous tissue is placed in the programmed cooling instrument, be cooled to-40 ℃～-60 ℃, last 20-80min with the velocity gradient of 0.5～2 ℃/min; 5, preserve: preserve in osseous tissue input-60～80 ℃ refrigerator after will lowering the temperature.
The present invention compared with prior art, its remarkable advantage is: the osteoarthrosis of the same race through gradient cooling, profound hypothermia preservation can keep Ultrastructural integrity, bone-inducting active, the biomechanical strength of bone, keep the chondrocyte that survives of some simultaneously, can increase the healing ability of transplanting back and host, reduce the incidence rate of complication, improve the function of joint after transplanting, for clinical providing possesses bioactive osteoarthrosis of the same race.
Four Figure of description
Accompanying drawing is the possess preparation of biological activity osseous tissue of the same race and the schematic flow sheet of store method of the present invention.
Five specific embodiment
Below in conjunction with accompanying drawing the present invention is further described.
Embodiment 1.In conjunction with the accompanying drawings, a kind of preparation and store method that possesses biological activity osseous tissue of the same race, its concrete implementation process is: obtain osteoarthrosis tissue of the same race under aseptic condition, reject soft tissue and periosteum, remove myeloid tissue, the attachment point of reserve part joint capsule, ligament and muscle, soak with chloroform methanol, pack behind the normal saline flushing, pretreated osseous tissue is placed in the programmed cooling instrument, velocity gradient with 2 ℃/min is cooled to-40 ℃, lasts 80min, will preserve in the osseous tissue input-80 ℃ case after the cooling.
The mechanics data of the osseous tissue of the same race in January are as follows after the present invention's preparation and preservation:
Tensile modulus of elasticity modulus of elasticity in comperssion Compression and Expansion
Handle
(Gpa) (Gpa) Poisson's ratio μ Poisson's ratio μ
The present invention's preparation,
18.23 13.63 0.32 0.345
Preserve bone of the same race
The Reilly test
17.9 18.3
The fresh normal femur
Data show with the elastic modelling quantity and the fresh bone of the osseous tissue of the same race of the present invention's preparation, preservation organizes no significant difference, illustrates that the biomechanics characteristic and the fresh bone of osseous tissue after preparation, preservation is close.
Embodiment 2.In conjunction with the accompanying drawings, a kind of preparation and store method that possesses biological activity osseous tissue of the same race is used for repairing, rebuilding behind the bone tumour resection guarantor's limb treatment of large segmental bone defect.
The patient, male, 16 years old.Right thigh bottom pain January, turn out to be right femoral inferior segment osteosarcoma through iconography and pathological examination, underwent operative is excised the neoplastic bone joint of complete right femoral inferior segment, 16 centimeters of resection lengths, the femoral inferior segment of leaving over after the excision is damaged, adopt the allogeneic femoral inferior segment of the inventive method preparation to carry out the skeleton reconstruction, not only excised tumor tissues up hill and dale, and kept the function of suffering from limb.Adopting homogeneous allogenic bone transplantation is one of main method of limb tumor guarantor limb treatment, and the limbs malignant bone tumor of foreign literature statistics more than 90% all obtains to protect the limb treatment.
Malignant bone tumor is protected the important step of limb treatment, is the large segmental bone defect of how rebuilding after malignant bone tumor excises, and a big section homogeneous bone transplantation is the most frequently used method.Its concrete implementation process is: obtain osteoarthrosis tissue of the same race under aseptic condition, reject soft tissue and periosteum, remove myeloid tissue, the attachment point of reserve part joint capsule, ligament and muscle soaks with chloroform methanol, packs behind the normal saline flushing, pretreated osseous tissue is placed in the programmed cooling instrument, velocity gradient with 1.0 ℃/min is cooled to-50 ℃, lasts 50min, will preserve in the osseous tissue input-100 ℃ refrigerator after the cooling.
The osseous tissue of the same race that adopts gradient cooling, profound hypothermia to preserve carries out transplant operation, has good healing ability clinically, usually four months after the transplanting can be observed the dense poly-phenomenon of transplantation site bone on isotope technetium 99 bone scanning sheets, special binding site at host bone and bone graft, the bone metabolism that shows bone graft is active, and prompting begins knitting with the host.Can observe area of new bone on the common X-line plain film in back 6 months in transplanting and form and held bone graft.Usually in the gap disappearance of the binding site of 8-10 month host bone and bone graft, show that host bone and bone graft heal fully, direct frozen bone and lyophilization bone photo ratio, the time of knitting shortens about 2-3 month, the wherein the longest time of following up a case by regular visits to reaches 36 months, and bone graft is not found the phenomenon of fracturing.At present clinical experiment used 60 surplus example, the incidence rate of various complication is lower than 25%.
Embodiment 3.In conjunction with the accompanying drawings, a kind of preparation and store method that possesses biological activity osseous tissue of the same race is used for the damaged reparation of bony metachromia bone, rebuilds treatment.Because of osteomyelitis cause bone damaged be common clinically infectious disease, autologous bone transplanting is the Therapeutic Method of using always, bring new misery to the patient on the one hand, often do not satisfy damaged needs from the body bone on the other hand, using osseous tissue transplanting of the same race is new Therapeutic Method.Its concrete implementation process is: obtain osteoarthrosis tissue of the same race under aseptic condition, reject soft tissue and periosteum, remove myeloid tissue, the attachment point of reserve part joint capsule, ligament and muscle soaks with chloroform methanol, packs behind the normal saline flushing, pretreated osseous tissue is placed in the programmed cooling instrument, velocity gradient with 1.5 ℃/min is cooled to-40 ℃, lasts 60min, will preserve in the osseous tissue input-90 ℃ refrigerator after the cooling.
The osseous tissue of the same race that adopts gradient cooling, profound hypothermia to preserve carries out the damaged prosthesis of infectious bone, and it is damaged to repair the bone that causes because of infection clinically, recovers the seriality of bone, simultaneously can effective infection control, and make osteomyelitis obtain rehabilitation.At present clinical experiment used 20 surplus example, clinical effective rate reaches 96%.For clinical treatment osteomyelitis provides a kind of reliable, economic reparation means.
Traumatic bone is damaged, bone tumor and infectious bone are damaged is clinical common diseases, needs bone to transplant usually and carries out repair and reconstruction.American-European countries is widely used, and annual approximately uses 1,000,000, and domesticly has approximately have only 20,000 the every year of record.The large population base of China is in developing country simultaneously, and the sickness rate height of diseases such as the wound of bone, infection and tumor has general application foundation.The preparation method of the osseous tissue of the same race that the present invention obtains, the homogeneous bone transplantation material that is obtained can keep Ultrastructural integrity, bone-inducting active, the biomechanical strength of bone.Can quicken to transplant back and host's healing ability, reduce the incidence rate of complication, improve the function of joint after transplanting,, be with a wide range of applications, can produce great economic and social benefit for clinical providing possesses bioactive osseous tissue of the same race.
Claims (3)
1, a kind of preparation and store method that possesses biological activity osseous tissue of the same race is characterized in that the steps include:
1.1 obtaining of bioactivity osseous tissue: under aseptic condition, obtain osteoarthrosis tissue of the same race;
1.2 the finishing of bioactivity osseous tissue: reject soft tissue and periosteum, remove myeloid tissue, the attachment point of reserve part joint capsule, ligament and muscle;
1.3 the defat of bioactivity osseous tissue: chloroform methanol soaks, and packs behind the normal saline flushing;
1.4 gradient cooling: pretreated osseous tissue is placed in the programmed cooling instrument, be cooled to-40 ℃～-60 ℃, last 20-80min with the velocity gradient of 0.5～2 ℃/min;
1.5 preserve: preserve in the osseous tissue input-80 after will lowering the temperature ℃～180 ℃ of refrigerators.
2, according to described preparation and the store method that possesses biological activity osseous tissue of the same race of claim 1, it is characterized in that pretreated osseous tissue is placed in the programmed cooling instrument, velocity gradient with 1～2 ℃/min is cooled to-40 ℃～-50 ℃, last 20-80min, will preserve in osseous tissue input-70～80 ℃ refrigerator after the cooling.
3, according to described preparation and the store method that possesses biological activity osseous tissue of the same race of claim 1, it is characterized in that pretreated osseous tissue is placed in the programmed cooling instrument, velocity gradient with 2 ℃/min is cooled to-40 ℃, last 80min, will preserve in the osseous tissue input-80 ℃ after the cooling～180 ℃ of refrigerators.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2003101060340A CN1528469A (en) | 2003-10-09 | 2003-10-09 | Method for preparing and preserving homogeneous bone tissue with biological activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2003101060340A CN1528469A (en) | 2003-10-09 | 2003-10-09 | Method for preparing and preserving homogeneous bone tissue with biological activity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1528469A true CN1528469A (en) | 2004-09-15 |
Family
ID=34304385
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2003101060340A Pending CN1528469A (en) | 2003-10-09 | 2003-10-09 | Method for preparing and preserving homogeneous bone tissue with biological activity |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1528469A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008014665A1 (en) * | 2006-07-26 | 2008-02-07 | The University Of Hong Kong | Intervertebral disk transplant and implantation method |
| CN100429975C (en) * | 2006-11-29 | 2008-11-05 | 四川大学华西医院 | Bone material preserving fluid and preparation method and application thereof |
| CN103491988A (en) * | 2011-04-12 | 2014-01-01 | 韩士生科（株）有限公司 | Graft materials derived from mammalian cartilage |
| CN103734124A (en) * | 2014-01-01 | 2014-04-23 | 张文涛 | Method for preserving orthopedic transplant material |
| CN111569150A (en) * | 2019-02-19 | 2020-08-25 | 上海兰玛生物材料有限公司 | Allogeneic bone repair material with bone induction activity and preparation method and application thereof |
-
2003
- 2003-10-09 CN CNA2003101060340A patent/CN1528469A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008014665A1 (en) * | 2006-07-26 | 2008-02-07 | The University Of Hong Kong | Intervertebral disk transplant and implantation method |
| CN100429975C (en) * | 2006-11-29 | 2008-11-05 | 四川大学华西医院 | Bone material preserving fluid and preparation method and application thereof |
| CN103491988A (en) * | 2011-04-12 | 2014-01-01 | 韩士生科（株）有限公司 | Graft materials derived from mammalian cartilage |
| CN103491988B (en) * | 2011-04-12 | 2015-07-22 | 韩士生科（株）有限公司 | Graft materials derived from mammalian cartilage |
| CN103734124A (en) * | 2014-01-01 | 2014-04-23 | 张文涛 | Method for preserving orthopedic transplant material |
| CN103734124B (en) * | 2014-01-01 | 2016-06-01 | 张文涛 | The store method of a kind of orthopedic transplant material |
| CN111569150A (en) * | 2019-02-19 | 2020-08-25 | 上海兰玛生物材料有限公司 | Allogeneic bone repair material with bone induction activity and preparation method and application thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102470032B (en) | Graft materials for surgical breast procedures | |
| Williams et al. | Custom-made titanium cranioplasty: early and late complications of 151 cranioplasties and review of the literature | |
| Vacanti et al. | Replacement of an avulsed phalanx with tissue-engineered bone | |
| Fliss et al. | Skull base reconstruction after anterior subcranial tumor resection | |
| CN101163512A (en) | Cartilaginiform and osteochondral sustitute comprising a multilayer structure and use thereof | |
| KR20190086033A (en) | Laminated tissue grafts composed of wharton's jelly and methods of making and using the same | |
| US8048618B2 (en) | Method for preparing and preserving a natural allograft intervertebral disk for humans | |
| US20120035744A1 (en) | Amnion and chorion constructs and uses thereof in joint repair | |
| Sadlik et al. | Knee cartilage regeneration with umbilical cord mesenchymal stem cells embedded in collagen scaffold using dry arthroscopy technique | |
| CN1528469A (en) | Method for preparing and preserving homogeneous bone tissue with biological activity | |
| Komender et al. | Preserved tissue allografts in reconstructive surgery | |
| CN109701077A (en) | A kind of micropore regenerating tissues matrix and its preparation and application | |
| RU86455U1 (en) | BIO ENGINEERING DESIGN | |
| Bentley | Articular tissue grafts. | |
| RU2309756C1 (en) | Method for treating false articulations due to transplantation of autologous mesenchymal stem cells and biotransplant for its application | |
| ES2340657B1 (en) | PROCEDURE FOR OBTAINING A TISSULAR ENGINEERING PRODUCT ORIENTED TO THE OSEO TISSUE REGENERATION. | |
| Eloesser | Implantation of joints | |
| Kim et al. | The biomechanical and biological effect of supercooling on cortical bone allograft | |
| Jancewicz et al. | Knee Osteochondral Defect Reconstruction With Autologous Bone Grafting and Mesenchymal Cell Transplantation | |
| RU2778615C1 (en) | Graft, a method for bone marrow autotransplantation to stimulate reparative bone regeneration and a device for carrying out transplantation | |
| RU2379002C1 (en) | Method of autoplasty of hyaline cartilage of knee joint | |
| Bischofreiter et al. | Arthroscopic Minced Cartilage Implantation for Chondral Lesion at the Glenoid in the Shoulder | |
| Breinan et al. | Reparative Tissues in Articular Cartilage Defects in a Canne Model Treated by Microfracture | |
| Tuli et al. | Effect of preimplantation treatment on the bone-forming potential of decalcified allogeneic and xenogeneic bone-matrix implants | |
| EP4090391A1 (en) | Multiple component grafts for treating tissue defects and methods for making and using same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |