CN103720932B - One boar Chinese medicine composition and its preparation method and application - Google Patents
One boar Chinese medicine composition and its preparation method and application Download PDFInfo
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Abstract
The invention discloses boar Chinese medicine composition and its preparation method and application.Said composition is by hay 11 ~ 33 weight portion under white hair, and Herba Taxilli 10 ~ 30 weight portion, Herba Cayatiae Japonicae 15 ~ 45 weight portion, the crude drug of Radix Serissae 9 ~ 27 weight portion is made.The preparation method of said composition is distilled by Herba Cayatiae Japonicae, collects distillate; Distillation test residue, residual liquid are added hay under white hair, Herba Taxilli, Radix Serissae decoct 2 ~ 3 times, collecting decoction is concentrated into thick shape, ethanol is adjusted to determining alcohol 60% ~ 70%, precipitation, filtration, medicinal liquid after concentrated adds ethanol again and is adjusted to determining alcohol 75% ~ 80%, precipitation, filter, filtrate concentrates, adding distil water cold preservation precipitates, and filters, concentrated; The distillate of Herba Cayatiae Japonicae and extraction concentrated solution are merged.This Chinese medicine composition is for the preparation of the herbal medicine thing for the treatment of pig blue-ear disease, circovirus disease, pseudorabies, piglet pujos blancos disease, swine enzootic pneumonia and Haemophilus parasuis, and side effect is little, safe and convenient to use.
Description
Technical field
The invention belongs to field of traditional Chinese medicine pharmacy, relate to a boar Chinese medicine composition, the invention still further relates to preparation method and the application of this Chinese medicine composition in the herbal medicine of the infectious disease such as preparation treatment pig blue-ear disease, Porcine circovirus desease and porcine pseudorabies of this Chinese medicine composition.
Background technology
Pandemic pig infectious disease in recent years, often two or more cause of disease secondary, concurrent reason.Common cause of disease has PRRS virus, pig circular ring virus, porcine pseudorabies virus etc., brings very large difficulty to Diagnosis and treatment.After sow infects reproductive and respiratory syndrome virus, porcine circovirus, pseudorabies virus, cause sow breeding difficulty (Sow abortion, product stillborn fetus, weak son etc.).After Infection in Piglets reproductive and respiratory syndrome virus, pseudorabies virus, mortality rate is very high.After Infection in Piglets porcine circovirus, although mortality rate is not high, Piglet Development is stagnated, and all cause very large loss to Swine Production, these swine diseases Western medicine are without therapeutical effect.China's natural resources of Chinese medicinal materials enriches, modern medicine study result shows, much Chinese medicine contains the materials such as abundant polysaccharide, alkaloids, organic acid, glycoside, volatile oil, the Chinese medicine preparation made with these materials is for animal, immune cell activated can be had, strengthen immunologic function, the effect of antiviral, antibacterium, anti-stress, inventor explores a kind of pig Chinese veterinary medicinal composition for the treatment of the infectious disease such as pig blue-ear disease, Porcine circovirus desease and pseudorabies, Be very effective, so far there are no the report of similar drugs control pig infectious disease of the present invention.
Summary of the invention
The object of the present invention is to provide a boar Chinese medicine composition.
Another object of the present invention is to provide the preparation method of above-mentioned pig Chinese medicine composition.
A further object of the invention is to provide the application of above-mentioned pig Chinese medicine composition in the herbal medicine thing of preparation treatment pig blue-ear disease, Porcine circovirus desease, pseudorabies, piglet pujos blancos disease, swine enzootic pneumonia and Haemophilus parasuis.
The object of the invention is to be implemented by following technical measures:
One boar Chinese medicine composition, said composition is made up of the crude drug of following weight portion: hay 11 ~ 33 weight portion under white hair, Herba Taxilli 10 ~ 30 weight portion, Herba Cayatiae Japonicae 15 ~ 45 weight portion, Radix Serissae 9 ~ 27 weight portion.
Above-mentioned composition is preferably made up of the crude drug of following weight portion: hay 11 ~ 18 weight portion under white hair, Herba Taxilli 10 ~ 15 weight portion, Herba Cayatiae Japonicae 15 ~ 20 weight portion, Radix Serissae 10 ~ 13 weight portion.
Above-mentioned pig Chinese medicine composition prepares through the following steps:
A, by Herba Cayatiae Japonicae adding distil water soak, distillation, collect distillate, for subsequent use;
B, in distillation test residue, residual liquid, add hay under white hair, Herba Taxilli, Radix Serissae, then decoct with water 2 ~ 3 times, after collecting decoction, be concentrated into thick shape, adding ethanol, to be adjusted to determining alcohol be 60% ~ 70%, abundant stirring and evenly mixing, precipitation, filter, filtrate recycling ethanol, reclaim the medicinal liquid after ethanol and add ethanol to be again adjusted to determining alcohol be 75% ~ 80%, stir evenly, precipitation, filter, after filtrate recycling ethanol, reconcentration is extremely without alcohol taste, the cold preservation of medicinal liquid adding distil water precipitates, and filters, and concentrated the obtaining of filtrate extracts concentrated solution.
C, extraction concentrated solution Herba Cayatiae Japonicae distillate and " b " step obtained merge, preparation.
It is injection and oral liquid that above-mentioned pig preferably prepares dosage form with Chinese medicine composition.
The preparation method of above-mentioned pig Chinese medicine composition comprises the following steps:
A, by Herba Cayatiae Japonicae adding distil water soak, distillation, collect distillate, for subsequent use;
B, in distillation test residue, residual liquid, add hay under white hair, Herba Taxilli, Radix Serissae, then decoct with water 2 ~ 3 times, after collecting decoction, be concentrated into thick shape, adding ethanol, to be adjusted to determining alcohol be 60% ~ 70%, abundant stirring and evenly mixing, precipitation, filter, filtrate recycling ethanol, reclaim the medicinal liquid after ethanol and add ethanol to be again adjusted to determining alcohol be 75% ~ 80%, stir evenly, precipitation, filter, after filtrate recycling ethanol, reconcentration is extremely without alcohol taste, the cold preservation of medicinal liquid adding distil water precipitates, and filters, and concentrated the obtaining of filtrate extracts concentrated solution;
C, extraction concentrated solution Herba Cayatiae Japonicae distillate and " b " step obtained merge, preparation.
The preparation method of described pig Chinese medicine composition, specifically can follow these steps to preparation:
A, in Herba Cayatiae Japonicae, add the distilled water immersion that Herba Cayatiae Japonicae medical material weighs 3 ~ 4 times distill after 24 ~ 30 hours, collect distillate, then by distillate redistillation, collect re-distilled liquid, for subsequent use.
B, to distillation test residue, hay under white hair is added in residual liquid, Herba Taxilli, Radix Serissae medical material, and hay under adding white hair, Herba Taxilli, the distilled water of Radix Serissae three taste medical material gross weight 4 ~ 5 times amount, soak after 24 ~ 30 hours and decoct 3 times, boil rear maintenance 0.5 ~ 1 hour at every turn, collecting decoction is also concentrated into thick shape, adding weight percent concentration is that to be adjusted to determining alcohol be 60% ~ 70% to 95% ethanol, abundant stirring and evenly mixing, precipitation, filter, filtrate recycling ethanol, reclaim the medicinal liquid after ethanol add again concentration be 95% ethanol to be adjusted to determining alcohol be 75% ~ 80%, stir evenly precipitation, filter, filtrate recycling ethanol reconcentration is extremely without alcohol taste, the cold preservation of medicinal liquid adding distil water precipitates, filter, concentrated the obtaining of filtrate extracts concentrated solution.
C, Herba Cayatiae Japonicae distillate and " b " are extracted concentrated solution merges, obtaining concentration is the medicinal liquid of every mL containing 1 ~ 2g crude drug amount, and tune pH7.0, makes injection or oral liquid.
This pig Chinese medicine composition is applied in the pig infectious disease such as treatment pig blue-ear disease, Porcine circovirus desease, pseudorabies, there is significant curative effect, can be applicable to the herbal medicine thing preparing treatment pig blue-ear disease, Porcine circovirus desease, pseudorabies, piglet pujos blancos disease, swine enzootic pneumonia and Haemophilus parasuis.
Beneficial effect of the present invention: the present invention draws traditional Chinese veterinary medicine marrow, under white hair, hay is the herb of labiate ajuga decumbens AjugadecumbensThunb., has heat-clearing and toxic substances removing, preventing phlegm from forming and stopping coughing, removing pathogenic heat from blood and dissipating blood stasis, antibacterial, antivirus action.Herba Taxilli Taxilluschinensis (DC.) Danscr. (Loranthaceae plant), invigorating the liver and kidney, bone and muscle strengthening, wind-damp dispelling, antiabortive.Cure mainly soreness of waist and knee joint, limbs are hemiplegia, frequent fetal movement, rheumatic arthralgia.Also has very strong antivirus action.Herba Cayatiae Japonicae Gayratiajaponica (Thunb.) Gagnep. (vitaceae), has clearing away heat-damp and promoting diuresis, removing toxic substances and promoting subsidence of swelling effect.Pharmacological research shows, the volatile oil of Herba Cayatiae Japonicae or water decoction all have remarkable antibacterial action to staphylococcus aureus, streptococcus pneumoniae etc., and have remarkable potentiation to body nonspecific immunity and specific immune function.The Herb of Radix Serissae Serissaserissoides (DC.) Druce (Maguireothamnus speciosus).Expelling wind and removing dampness, heat-clearing and toxic substances removing.Cure mainly flu, cough, enteritis, dysentery, hepatitis, swollen ulcer drug, traumatic injury.The present invention not only has good preventive and therapeutic effect to pig blue-ear disease, pseudorabies, Porcine circovirus desease, and also have good curative effect to piglet pujos blancos disease, swine enzootic pneumonia and Haemophilus parasuis, 4 kinds of compatibility of drugss, reach the effect for the treatment of both the principal and secondary aspects of a disease.
Curative effect of the present invention is further illustrated below with regard to effect experiment.
One, In Vitro Anti porcine pseudorabies virus of the present invention experimental study
1, SPF tests Embryo Gallus domesticus purchased from Shandong experiment kind of chicken house.
2, pseudorabies sickly complexion A strain AV25 strain is provided by China Veterinery Drug Inspection Office, strain normal saline dilution, and inoculation SPF Embryo Gallus domesticus, collects allantoic fluid, be for experiment.The HA of chick embryo allantoic liquid tires as 6log
2.
3, this test of medicine take the present composition as test drug; Ribavirin is Western medicine contrast medicine, and by Jinan, favorable to the people pharmaceutical Co. Ltd produces, and is made into 0.05g/mL concentration.Tannic acid is produced by Shanghai Ling Feng chemical reagent company limited.
4, laboratory animal and erythrocytic preparation are grown up Balb/c mice, are purchased from Yangzhou University's Experimental Animal Center.Erythrocytic preparation: cut off eyeball of mouse with eye scissors, expose optical fundus blood vessel, take a blood sample several and wash 3 times with PBS in the centrifuge tube being added with anticoagulant, be then made into 10% erythrocyte with PBS, with 1/40000 concentration tan-liquor equal-volume Homogeneous phase mixing, centrifugal in 37 DEG C of effects 1 hour, then wash 3 times with PBS, centrifugal with 1500r/min, each 5min, adds PBS and is made into 0.5% red cell suspension during use.
5, test grouping instar chicken embryo on the 10th 52 pieces, be divided into 4 groups at random, concrete grouping situation is in table 1.
Table 1 Embryo Gallus domesticus grouping situation
6, test method
(1) minimum lethal dose measures viral allantoic fluid dilution different multiples, inoculates non-ly to exempt from Embryo Gallus domesticus, draws embryo's minimum lethal dose.Through measuring, the dilution factor of Embryo Gallus domesticus virus allantoic fluid minimum lethal dose is 1: 50, formal test inoculation dilution factor with 1: 50 allantoic fluid.
(2) toxicity trial Chinese medicine original liquid normal saline makes doubling dilution, the medicinal liquid of Chinese medicine stock solution and variable concentrations is inoculated in 3 piece of 10 age in days chick embryo allantoic cavity respectively, every 0.2mL, put 37.8 DEG C of incubations 72 hours, every day observes survival condition, the highest liquor strength that Embryo Gallus domesticus is all survived is formal test initial concentration, establishes saline control group simultaneously, medicine to embryonic toxicity result of the test in table 2.
Table 2 Chinese medicine is to embryonic toxicity experimental result (n=4)
★ denominator is inoculation embryo number, and molecule is survival embryo number.
(3) the medicine of the present invention dilution factor of inhibitory action mensuration 1:50 allantoic fluid of breeding pseudorabies virus and the drug level of table 2 are as the foundation of inoculation.Concrete grammar is as follows.
Test I group of each chick embryo allantoic cavity Simultaneous vaccination medicine of the present invention (preparing by embodiment 1 method) 0.2mL, viral dilution liquid 0.1mL.
Test II group of each chick embryo allantoic cavity and meet Western medicine 0.2mL simultaneously, viral dilution liquid 0.1mL.
Test III group of each chick embryo allantoic cavity Simultaneous vaccination normal saline 0.2mL, viral dilution liquid 0.1mL.
Above-mentioned Embryo Gallus domesticus is hatched in 37.8 DEG C of calorstats, and every day shines egg 2 ~ 3 times, discards dead germ in 24 hours.Collect in 72 hours live embryo and 24 ~ 72 hours dead germ allantoic fluids, cryopreservation is for subsequent use.The virus titer of chick embryo allantoic liquid is often organized with blood clotting (HA) testing inspection.
7, Chinese medicine inhibitory action that pseudorabies virus is bred
Ith group of Embryo Gallus domesticus dead 1 piece at 24 ~ 72 hours, survival in 72 hours 12 pieces, collect 1 piece of dead Embryo Gallus domesticus and 12 pieces of chick embryo allantoic liquids of surviving, HA titre is 0;
IIth group of Embryo Gallus domesticus dead 10 pieces at 24 ~ 72 hours, survival in 72 hours 3 pieces, collect the allantoic fluid of 10 pieces of dead germs and 3 pieces of embryos alive, HA titre is 4log
2;
IIIth group of Embryo Gallus domesticus dead 13 pieces at 24 ~ 72 hours, collect 13 pieces of allantoic fluids, HA titre is 8log
2;
IVth group in 72 hours without chicken embryo death, collect all embryo allantoic liquids alive, HA titre is 0, and detailed results is in table 3.
The inhibitory action measurement result that table 3 Chinese medicine is bred pseudorabies virus
Compare with the IIIth group, * * P < 0.01
Two, the present invention is to the therapeutic test of experimental pseudorabies
1, experimental animal purchases 30 age in days kunming mice two batches, the 1st batch 25, for viral kill quantitative determination.2nd batch 66, be divided into 6 groups, often organize 11, i.e. invention prevention group and treatment by Chinese herbs group (preparing by embodiment 2 method), Western medicine prevention group and treatment group, infect matched group, blank group.
2, test method
(1) minimum lethal dose measures with 30 age in days kunming mice 25, be divided into 5 groups at random, carry out minimum lethal dose mensuration with the pseudorabies virus allantoic fluid through Embryo Gallus domesticus rejuvenation.Result of the test, minimum lethal dose is that allantoic fluid 30 times dilution 0.2mL/ is only oral.
(2) inoculate the mice not medication of blank group, do not inoculate allantoic fluid.All the other mices of 5 groups at one time in every only oral minimum lethal dose be after measured the allantoic fluid 0.2mL of 1: 30 dilution.
(3), while administration time invention prevention group inoculation allantoic fluid, start with Chinese medicine of the present invention (preparing by embodiment 2 method) oral, every each 1mL of mice, every day 2 times, is used in conjunction 5 days.Invention treatment group is in the medication (preparing by embodiment 2 method) that (occurs symptom) after 2 days of inoculation allantoic fluid, and every each 1mL of mice, medication every day 2 times, is used in conjunction 5 days.Western medicine prevention group is while inoculation allantoic fluid, and start with the ribavirin of 0.05g/mL oral, every 1mL, every day 2 times, is used in conjunction 5 days.Western medicine group, in the medication that (occurs symptom) after 2 days of inoculation allantoic fluid, oral with the ribavirin of 0.05g/mL, every each 1mL of mice, medication every day 2 times, is used in conjunction 5 days.Infect the mice of matched group and only inoculate allantoic fluid, need not any medicine, the results are shown in Table 4.
The prevention of table 4 pseudorabies and therapeutic outcome unit: a %
Three, the present invention is to the clinical treatment outcome of pig blue-ear disease and circovirus disease
On November 10th, 2013, there is 48 age in days piglet 65 on scale pig farm, Anhui Chuzhou, occurs that spirit is depressed, loss of appetite or absolutely useless.November 11, body temperature rises to about 41 DEG C, dyspnea, and whole skin is rubescent, diarrhoea, inguinal lymphadenopathy.Cut open inspection 2, cut open inspection change: the congested swelling of larynx, lung be that point-like and speckle shape are hemorrhage, and tangent plane is that marble sample changes, kiney edema, hemorrhage, in khaki.There is a large amount of hydrops in pericardium and thoracic cavity, heart is bled profusely a little.Mesenteric lymph node, inguinal lymph nodes are hemorrhage.Get pathological material of disease and send Anhui academy of agricultural sciences, use RT-PCR(inverse transcription polymerase chain reaction) detect, be diagnosed as PRRS virus and porcine circovirus mixed infection.Use medicine of the present invention (preparing according to embodiment 1 method) to treat immediately, and be control drug with astragalus polysaccharides, treat 4 days altogether, every each 5mL of piglet, medication every day 2 times, therapeutic outcome is in table 5.
Table 5 pig blue-ear disease and porcine circovirus mixed infection efficacy result unit: head %
Seen by this clinical practice result, injections treatment pig blue-ear disease of the present invention, circovirus disease, curative effect is than astragalus polysaccharides successful.Show Chinese medicine preparation of the present invention, treatment pig virus infectious disease has very high curative effect.
Four, the present invention is to the curative effect of other infectious disease of pig
The present invention's (preparing by embodiment 4 method) to the curative effect of other infectious disease of pig in table 6.
The curative effect statistical unit of other infectious disease of table 6 pig: head %
Pig Chinese medicine composition of the present invention is pure Chinese medicine formula, and side effect is little, safe and convenient to use, absorbs fast.All be difficult to produce drug resistance more than 90% to the cure rate of virus and bacterial infectious disease, and avoid the generation of residue of veterinary drug and social effects of pollution.
Detailed description of the invention
Below in conjunction with specific embodiment, the invention will be further described:
Embodiment 1:
Prescription: hay 13g, Herba Taxilli 12g, Herba Cayatiae Japonicae 20g, Radix Serissae 11g under white hair
Concrete preparation process is as follows:
A, Herba Cayatiae Japonicae medical material is added the distilled water immersion 24 hours of Herba Cayatiae Japonicae weight 3 times, distill after medical material is fully expanded, collect distillate, obtain the distillate of medical material gross weight 2 times, again by distillate redistillation 1 time, collect and obtain this kind of medical material gross weight 1/2 distillate, bottling sterilizing is for subsequent use.
B, by hay under distillation test residue and residual liquid Jia Baimao, Herba Taxilli, Radix Serissae medical material, and hay under adding white hair, Herba Taxilli, the distilled water of Radix Serissae three taste medical material gross weight 4 times, soak after 24 hours and decoct 3 times, boil rear maintenance 0.5 hour at every turn, collecting decoction is also concentrated into thick shape, add weight percent concentration be 95% ethanol be adjusted to concentration of alcohol 60%, abundant stirring and evenly mixing, precipitate more than 48 hours, filter, filtrate recycling ethanol, reclaim the medicinal liquid after ethanol add again concentration be 95% ethanol be adjusted to determining alcohol 75%, stir evenly, precipitate 48 hours, filter, filtrate recycling ethanol, reconcentration is extremely without alcohol taste, in the medicinal liquid obtained, add its 4 times amount distilled water cold preservation precipitate 48 hours, filter, concentrated the obtaining of filtrate extracts concentrated solution,
C, Herba Cayatiae Japonicae distillate and " b " are extracted concentrated solution merges, obtaining concentration is the medicinal liquid of every mL containing crude drug 1.5g, and tune pH7.0, makes injection, subpackage, sterilizing and get final product.
Embodiment 2:
Prescription: hay 11g, Herba Taxilli 10g, Herba Cayatiae Japonicae 18g, Radix Serissae 13g under white hair
Concrete preparation process is as follows:
A, Herba Cayatiae Japonicae medical material is added the distilled water immersion 24 hours of Herba Cayatiae Japonicae weight 3 times, distill after medical material is fully expanded, collect distillate, obtain the distillate of medical material gross weight 2 times, again by distillate redistillation 1 time, collect and obtain this kind of medical material gross weight 1/2 distillate, bottling sterilizing is for subsequent use.
B, by hay under distillation test residue and residual liquid Jia Baimao, Herba Taxilli, Radix Serissae three taste medical material, and hay under adding white hair, Herba Taxilli, the distilled water of Radix Serissae three taste medical material gross weight 4 times, soak after after 24 hours and decoct 3 times, boil rear maintenance more than 0.5 hour at every turn, collecting decoction is also concentrated into thick shape, add weight percent concentration be 95% ethanol be adjusted to concentration of alcohol 60%, abundant stirring and evenly mixing, precipitate more than 48 hours, filter, filtrate recycling ethanol, reclaim the medicinal liquid after ethanol add again concentration be 95% ethanol be adjusted to determining alcohol 75%, stir evenly precipitation 48 hours, filter, filtrate recycling ethanol, reconcentration is extremely without alcohol taste, in the medicinal liquid obtained, add its 4 times amount distilled water cold preservation precipitate 48 hours, filter, concentrated the obtaining of filtrate extracts concentrated solution,
C, Herba Cayatiae Japonicae distillate and " b " are extracted concentrated solution merges, obtaining concentration is the medicinal liquid of every mL containing crude drug 1g, and tune pH7.0, makes oral liquid, subpackage, sterilizing and get final product.
Embodiment 3:
Prescription: hay 22g, Herba Taxilli 20g, Herba Cayatiae Japonicae 30g, Radix Serissae 18g under white hair
Concrete preparation process is as follows:
A, Herba Cayatiae Japonicae medical material is added the distilled water immersion 30 hours of Herba Cayatiae Japonicae weight 4 times, distill after medical material is fully expanded, collect distillate, obtain the distillate of this medical material gross weight 2 times, again by distillate redistillation 1 time, collect 1/2 distillate obtaining this kind of medical material gross weight, bottling sterilizing is for subsequent use.
B, by hay, Herba Taxilli, Radix Serissae three taste medical material under distillation test residue and residual liquid Jia Baimao, and the distilled water of hay, Herba Taxilli, Radix Serissae three taste medical material gross weight 5 times under adding white hair, immersion immersion is rear after 24 hours to decoct 3 times, boil rear maintenance 1 hour, collecting decoction is also concentrated into thick shape at every turn, and adding weight percent concentration is that 95% ethanol is adjusted to concentration of alcohol 65%, abundant stirring and evenly mixing, precipitate 60 hours, filter, filtrate recycling ethanol.Reclaim the medicinal liquid after ethanol add again concentration be 95% ethanol be adjusted to determining alcohol 80%, stir evenly precipitation 48 hours, filter, filtrate recycling ethanol, reconcentration is to without alcohol taste, and medicinal liquid adds 2 times amount distilled water cold preservations and precipitates 24 hours, filters, and filtrate is concentrated obtains extraction concentrated solution.
C, Herba Cayatiae Japonicae distillate and " b " are extracted concentrated solution merges, obtaining concentration is the medicinal liquid of every mL containing crude drug 2g, tune pH7.0, make oral liquid, subpackage, sterilizing and get final product.
Embodiment 4:
Prescription: hay 18g, Herba Taxilli 15g, Herba Cayatiae Japonicae 16g, Radix Serissae 10g under white hair
Concrete preparation process is as follows:
A, Herba Cayatiae Japonicae medical material is added the distilled water immersion 27 hours of Herba Cayatiae Japonicae medical material gross weight 3 times, distill after medical material is fully expanded, collect distillate, obtain the distillate of this medical material gross weight 2 times, again by distillate redistillation 1 time, collect 1/2 distillate obtaining this kind of medical material gross weight, bottling sterilizing is for subsequent use.
B, by hay, Herba Taxilli, Radix Serissae three taste medical material under distillation test residue and residual liquid Jia Baimao, and the distilled water of hay, Herba Taxilli, Radix Serissae three taste medical material gross weight 5 times under adding white hair, soak after 24 hours and decoct 3 times, boil rear maintenance 1 hour, collecting decoction is also concentrated into thick shape at every turn, and adding weight percent concentration is that 95% ethanol is adjusted to concentration of alcohol 70%, abundant stirring and evenly mixing, precipitate 48 hours, filter, filtrate recycling ethanol.The medicinal liquid reclaimed after ethanol adds 95% ethanol again and is adjusted to determining alcohol 80%, stirs evenly precipitation 48 hours, filters, filtrate recycling ethanol, reconcentration, to without alcohol taste, adds its 3 times amount distilled water cold preservation and precipitates 24 hours in the medicinal liquid obtained, filter, concentrated the obtaining of filtrate extracts concentrated solution.
C, Herba Cayatiae Japonicae distillate and " b " are extracted concentrated solution merges, reach the concentration of every mL containing crude drug 1.5g, tune pH7.0, makes injection, subpackage, sterilizing and get final product.
Claims (6)
1. a boar Chinese medicine composition, is characterized in that said composition is made up of the crude drug of following weight portion: Herba Ajugae 11 ~ 33 weight portion, Herba Taxilli 10 ~ 30 weight portion, Herba Cayatiae Japonicae 15 ~ 45 weight portion, Radix Serissae 9 ~ 27 weight portion.
2. pig Chinese medicine composition according to claim 1, it is characterized in that said composition is made up of the crude drug of following weight portion: Herba Ajugae 11 ~ 18 weight portion, Herba Taxilli 10 ~ 15 weight portion, Herba Cayatiae Japonicae 15 ~ 20 weight portion, Radix Serissae 10 ~ 13 weight portion.
3. pig Chinese medicine composition according to claim 1, is characterized in that dosage form prepared by described compositions is injection or oral liquid.
4. the preparation method of pig Chinese medicine composition according to claim 1 and 2, is characterized in that the method is:
A, by Herba Cayatiae Japonicae adding distil water soak, distillation, collect distillate, for subsequent use;
B, in distillation test residue, residual liquid, add Herba Ajugae, Herba Taxilli, Radix Serissae, then decoct with water 2 ~ 3 times, after collecting decoction, be concentrated into thick shape, adding ethanol, to be adjusted to determining alcohol be 60% ~ 70%, abundant stirring and evenly mixing, precipitation, filter, filtrate recycling ethanol, reclaim the medicinal liquid after ethanol and add ethanol to be again adjusted to determining alcohol be 75% ~ 80%, stir evenly, precipitation, filter, after filtrate recycling ethanol, reconcentration is extremely without alcohol taste, the cold preservation of medicinal liquid adding distil water precipitates, and filters, and concentrated the obtaining of filtrate extracts concentrated solution;
C, extraction concentrated solution Herba Cayatiae Japonicae distillate and " b " step obtained merge, preparation.
5. the preparation method of pig Chinese medicine composition according to claim 4, is characterized in that the method specifically follows these steps to preparation:
A, the distilled water immersion adding Herba Cayatiae Japonicae medical material gross weight 3 ~ 4 times in Herba Cayatiae Japonicae distilled after 24 ~ 30 hours, collected distillate, then by distillate redistillation, collected and heavily steam liquid, for subsequent use;
B, to distillation test residue, Herba Ajugae is added in residual liquid, Herba Taxilli, Radix Serissae three taste medical material, and add Herba Ajugae, Herba Taxilli, the distilled water immersion of Radix Serissae three taste medical material gross weight 4 ~ 5 times amount decocted 3 times after 24 ~ 30 hours, boil rear maintenance 0.5 ~ 1 hour at every turn, collecting decoction is also concentrated into thick shape, adding weight percent concentration is that to be adjusted to determining alcohol be 60% ~ 70% to 95% ethanol, abundant stirring and evenly mixing, precipitation, filter, filtrate recycling ethanol, reclaim the medicinal liquid after ethanol add again concentration be 95% ethanol to be adjusted to determining alcohol be 75% ~ 80%, stir evenly, precipitation, filter, filtrate recycling ethanol, reconcentration is extremely without alcohol taste, the cold preservation of medicinal liquid adding distil water precipitates, filter, concentrated the obtaining of filtrate extracts concentrated solution,
C, Herba Cayatiae Japonicae distillate and " b " are extracted concentrated solution merges, obtaining concentration is the medicinal liquid of every mL containing 1 ~ 2g crude drug amount, and tune pH7.0, makes injection or oral liquid.
6. the application of Chinese medicine composition in the herbal medicine thing of preparation treatment pig blue-ear disease, Porcine circovirus desease, pseudorabies, piglet pujos blancos disease, swine enzootic pneumonia or Haemophilus parasuis of the pig described in claim 1 or 2.
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| CN106361987A (en) * | 2016-09-23 | 2017-02-01 | 佛山科学技术学院 | Traditional Chinese medicine composition for pigs and preparation method and application of traditional Chinese medicine composition for pigs |
| CN107320536A (en) * | 2017-07-10 | 2017-11-07 | 江苏农牧科技职业学院 | Prevent and treat Chinese medicine composition of pig blue-ear disease and preparation method thereof |
| CN110420274A (en) * | 2019-09-12 | 2019-11-08 | 江苏农牧科技职业学院 | A kind of pharmaceutical composition and its preparation method and application for treating canine coronavirus disease |
| CN115252725A (en) * | 2022-08-11 | 2022-11-01 | 山东优维药业有限公司 | Medicament for treating haemophilus parasuis disease |
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