CN103719642A - Mulberry leaf storage preservative with effect of increasing silkworm cocoon yield - Google Patents
Mulberry leaf storage preservative with effect of increasing silkworm cocoon yield Download PDFInfo
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- CN103719642A CN103719642A CN201310732797.XA CN201310732797A CN103719642A CN 103719642 A CN103719642 A CN 103719642A CN 201310732797 A CN201310732797 A CN 201310732797A CN 103719642 A CN103719642 A CN 103719642A
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Abstract
The invention discloses a mulberry leaf storage preservative with the effect of increasing silkworm cocoon yield. The mulberry leaf storage preservative is prepared through mixing a reagent A and a reagent B according to the volume ratio of 1: 1; the reagent A per litre is prepared from the following ingredients in mass: 40g-60g of vitamin C, 5g of NaCl, 1,000mg of norfloxacin hydrochloride, 10g-30g of chitosan, 30g-50g of acetic acid, 20g-40g of citric acid, 40g-60g of saccharose and the balance being water; the reagent B is an aqueous solution of D. radiodurans R1 (with the collection number of ATCC13939 (American Type Culture Collection 13939)) culture, wherein the mass content of the D. radiodurans R1 culture in the aqueous solution is 0.6%-1%.
Description
Technical field
The invention belongs to storage technique field, relate to a kind of mulberry leaf preservation agent for storing with silk cocoon production-increasing function and preparation method thereof.
Background technology
China is the cradle of world's sericulture, has the long history of 5,000 years.Planting mulberry and breeding silkworm were once the important means of pillar and the peasant programme of vast rural economic development; But along with the fast development of rural economy, the swift and violent emergence of second and third industry, a large amount of rural laborers pour in industrial and commercial enterprises, and traditional labor-intensive production-sericulture is also subject to enormous impact, in the urgent need to a kind of rearing with less number of feeds a day Technique of Laborsaving Rearing.But rearing with less number of feeds a day sericulture technology causes that mulberry leaf are exposed to the external world for a long time, the percentage of water loss of mulberry leaf is increased, thereby affect silkworm digesting and assimilating the edible and nutrition of the mulberry leaf that wither.So reduce mulberry leaf percentage of water loss in the rearing with less number of feeds a day, batch production sericulture process, keep that mulberry leaf are permanent fresh has just become a problem demanding prompt solution.Though cover the moisture that can reduce to a certain extent mulberry leaf by film, evaporate, the degree reducing is limited, and to the mulberry operation such as remove sand, all will remove and cover film at every turn, wastes time and energy; Also have the chemical agent of interpolation to carry out mulberry leaf fresh-keeping (patent No. is CN1158939), it is mainly by malic acid, citric acid, sorbic acid, hydrochloric acid, phytic acid, dehydration acetate, Na
2sO
3, NaH
2pO
4, K
2s
2o
4, the chemical substance such as antibiotic forms for NaCl, propane diols, agar, carragheen, gelatin, poultry, though the method can reduce the evaporation of mulberry leaf moisture in a way, but environment is had certain pollution, growing of silkworm also had to certain side effect, and function singleness.
The radioresistant bacterium D.radiodurans R1You American National DSMZ that preserving number is ATCC NO.13939 provides, and in invention < < radioresistant cocci anti contravariance related gene that this bacterial strain is 200910098256.X at application number and application > > thereof, has clearly and informs; That is, for deriving from radioresistant cocci bacterial strain (DEINOCOCCUSRADIODURANS R1, ATCC NO.13939, purchased from American Type Culture Collecti).
Summary of the invention
The technical problem to be solved in the present invention is to provide a kind of mulberry leaf preservation agent for storing with silk cocoon production-increasing function.
In order to solve the problems of the technologies described above, the invention provides a kind of mulberry leaf preservation agent for storing with silk cocoon production-increasing function, this mulberry leaf preservation agent for storing is obtained by mixing according to the volume ratio of 1:1 by reagent A and reagent B;
Every L reagent A is grouped into by the one-tenth of following quality:
Vitamin C 40～60g, NaCl5g, norfloxacin hydrochloride 1000mg, shitosan 10～30g, acetic acid 30～50g, citric acid 20～40g, sucrose 40～60g, surplus is water (being for example distilled water);
Described reagent B is the aqueous solution that radioresistant bacterium D.radiodurans R1 cultivates thing; In the described aqueous solution, the mass content of radioresistant bacterium D.radiodurans R1 cultivation thing is 0.6%～1%.
As the improvement with the mulberry leaf preservation agent for storing of silk cocoon production-increasing function of the present invention: radioresistant bacterium D.radiodurans R1 cultivates the preparation method of thing for comprising the following steps:
1), by preserving number, being ATCC NO.13939, radioresistant bacterium D.radiodurans R1(You American National DSMZ provides) bacterium liquid is by 5%(volume ratio) inoculum concentration access defatted soybean culture medium in carry out fermented and cultured, in 29.5～30.5 ℃, cultivate 58～62h(and be preferably 30 ℃ and cultivate 60h);
2), the fermented and cultured product of step 1) gained is preferably to the centrifugal 5min in 5500rpm in the centrifugal 4.5～5.5min(of 5000～6000rpm), remove supernatant, the radioresistant bacterium wet thallus of gained is paved into 0.25～0.35cm(equably and is preferably 0.3cm) thickness, at frequency 600MHz, under the condition of electric-field intensity 8000V/m, process 38～42 seconds (being preferably 40 seconds), make clasmatosis, thereby obtain through broken radioresistant bacterium;
3), by described, through broken radioresistant bacterium, be placed in material disc, put into and in cold wind drier, carry out cold air drying, chilling temperature is controlled at 20～25 ℃, wind speed 1.5～2.0m/s, relative humidity 10～15%, be dried to water content 6～8%, obtain radioresistant bacterium D.radiodurans R1 and cultivate thing.
As the further improvement with the mulberry leaf preservation agent for storing of silk cocoon production-increasing function of the present invention:
Defatted soybean culture medium consists of the following composition: fineness is 60-80 object defatted soy flour 20g, glucose 2g, and sodium nitrate 2g, yeast extract 1g, adding distil water is to 1000mL.Before use, carry out conventional sterilization treatment (in 1.1 atmospheric pressure, 121 ℃ at sterilizing 20min).
Remarks explanation:
The radioresistant bacterium D.radiodurans R1(You American National DSMZ using in the present invention provides, preserving number is ATCC NO.13939) be and derive from radioresistant cocci bacterial strain (DEINOCOCCUSRADIODURANS R1, ATCC NO.13939, purchased from American Type Culture Collecti), in invention < < radioresistant cocci anti contravariance related gene that this bacterial strain is 200910098256.X at application number and application > > thereof, have clearly and inform.
The present invention compared with prior art, has the following advantages and effect:
(1) raw material is easy to get, and preparation method is simple, and handled easily is cheap;
(2) the mulberry leaf preservation agent for storing that utilizes the organic substances such as shitosan to make, be sprayed at mulberry leaf surface and form layer protecting film, can prevent or reduce the evaporation of mulberry leaf moisture, thereby keep mulberry leaf fresh for a long time, under normal temperature condition, than untreated new fresh mulberry leaf, can reduce the evaporation 47.01～55.93% of mulberry leaf moisture, effectively extend mulberry leaf fresh keeping time, solved the more technical barrier of mulberry leaf dehydration in the Labor-saving that silkworm educates for a day a time or a day two times are educated and batch production sericulture technical process;
(3) changed existing antistaling agent and can only make the fresh-keeping single effect of mulberry leaf, by spraying mulberry leaf preservation agent for storing when keeping mulberry leaf permanent fresh, also can significantly improve silk cocoon amount and cocoon shell weight, cocoon weight improves 6.9%～13.1%, and cocoon shell weight improves 5.2%～12.4%;
(4) to silkworm nonhazardous, environmentally safe.
In sum, the mulberry leaf preservation agent for storing with silk cocoon production-increasing function of the present invention, both can reduce mulberry leaf percentage of water loss, keep the fresh for a long time of mulberry leaf; Make, after the mulberry leaf of this preservation agent for storing of the edible sprinkling of silkworm, to there is the effect of the yield and quality that increases silk cocoon simultaneously.Use antistaling agent of the present invention can make silkworm educates for one day one time or one day two times are educated the Technique of Laborsaving Rearing possibility that becomes, have more significant economic benefit and social benefit, market prospects are wide.
During the actual use of mulberry leaf preservation agent for storing with silk cocoon production-increasing function of the present invention, with sprayer, be evenly sprayed at the blade back two sides, blade face of mulberry leaf, to squirt, do not drip and be advisable.
The specific embodiment
Below in conjunction with specific embodiment, the present invention is described further, but protection scope of the present invention is not limited in this.
The preparation method of the mulberry leaf preservation agent for storing of EXAMPLE l, a kind of silk cocoon production-increasing function, comprises the following steps:
1), reagent preparation A:
Every L reagent A is grouped into by the one-tenth of following quality:
Vitamin C 40g, NaCl5g, norfloxacin hydrochloride 1000mg, shitosan 10g, acetic acid 30g, citric acid 20g, sucrose 40g, surplus is water (distilled water).
The preparation method of mentioned reagent A is: get the shitosan of 10g, be dissolved in the 500m1 distilled water containing 30g acetic acid, magnetic agitation to shitosan dissolves completely, obtains shitosan-acetic acid solution; Then in above-mentioned shitosan-acetic acid solution, add citric acid 20g, norfloxacin hydrochloride 1000mg, vitamin C 40g, NaCl5g, sucrose 40g, distilled water is settled to 1000ml.
2), reagent preparation B:
First prepare radioresistant bacterium D.radiodurans R1 and cultivate thing, preparation method is for carrying out successively following steps:
1., the radioresistant bacterium D.radiodurans R1(You American National DSMZ of activation is provided, preserving number is ATCC NO.13939) bacterium liquid is by 5%(volume ratio) inoculum concentration access defatted soybean join culture medium (defatted soybean culture medium preparation: consist of in every 1000mL culture medium: fineness is 60-80 object defatted soy flour 20g, glucose 2g, sodium nitrate 2g, yeast extract 1g, adding distil water is to 1000mL, in 1.1 atmospheric pressure, sterilizing 20min at 121 ℃) in, carry out fermented and cultured, cultivate 60h for 30 ℃;
2., by the fermented and cultured product of step 1) gained in the centrifugal 5min of 5500rpm, remove supernatant, the radioresistant bacterium wet thallus of gained is paved into equably to the thickness of about 0.3cm, at frequency 600MHz, under the condition of electric-field intensity 8000V/m, process 40 seconds, make clasmatosis, thereby obtain through broken radioresistant bacterium;
3., by above-mentioned, through broken radioresistant bacterium, be placed in material disc, put in cold wind drier and carry out cold air drying, chilling temperature is controlled at 20-25 ℃, wind speed 1.5-2.0m/s, relative humidity 10-15%, is dried to water content 6～8%, obtains radioresistant bacterium D.radiodurans R1 and cultivates thing.
Then, get above-mentioned radiation bacterium D.radiodurans R1 cultivation thing 6g and be dissolved in 994g distilled water, be stirred to radiation bacterium D.radiodurans R1 and cultivate thing dissolving, obtain radiation bacterium D.radiodurans R1 and cultivate the reagent B that amount content is 0.6%.
3), reagent A and reagent B are evenly mixed according to the volume ratio of 1:1, obtain the mulberry leaf preservation agent for storing with silk cocoon production-increasing function of the present invention.
The preparation method of the mulberry leaf preservation agent for storing of embodiment 2, a kind of silk cocoon production-increasing function,
1), every L reagent A is grouped into by the one-tenth of following quality:
Vitamin C 50g, NaCl5g, norfloxacin hydrochloride 1000mg, shitosan 20g, acetic acid 40g, citric acid 30g, sucrose 50g, surplus is water.
2), get radiation bacterium D.radiodurans R1 and cultivate thing 8g and be dissolved in 992g distilled water, be stirred to radiation bacterium D.radiodurans R1 and cultivate thing and dissolve, obtain radiation bacterium D.radiodurans R1 and cultivate the reagent B that amount content is 0.8%.
Radiation bacterium D.radiodurans R1 cultivates the preparation method of thing with embodiment 1.
3), reagent A and reagent B are evenly mixed according to the volume ratio of 1:1, obtain the mulberry leaf preservation agent for storing with silk cocoon production-increasing function of the present invention.
The preparation method of the mulberry leaf preservation agent for storing of embodiment 3, a kind of silk cocoon production-increasing function,
1), every L reagent A is grouped into by the one-tenth of following quality:
Vitamin C 60g, NaCl5g, norfloxacin hydrochloride 1000mg, shitosan 30g, acetic acid 50g, citric acid 40g, sucrose 60g, surplus is water.
2), get radiation bacterium D.radiodurans R1 and cultivate thing 10g and be dissolved in 990g distilled water, be stirred to radiation bacterium D.radiodurans R1 and cultivate thing and dissolve, obtain radiation bacterium D.radiodurans R1 and cultivate the reagent B that amount content is 1.0%.
Radiation bacterium D.radiodurans R1 cultivates the preparation method of thing with embodiment 1.
3), reagent A and reagent B are evenly mixed according to the volume ratio of 1:1, obtain the mulberry leaf preservation agent for storing with silk cocoon production-increasing function of the present invention.
Experiment 1, mulberry leaf percentage of water loss and feeding effect:
Utilize the mulberry leaf preservation agent for storing that above-described embodiment 1～3 obtains with sprayer, to be evenly sprayed at the blade back two sides, blade face of mulberry leaf, to squirt, do not drip and be advisable, the mulberry leaf of gained are as experimental group 1～3;
With distilled water, spray (be evenly sprayed at the blade back two sides, blade face of mulberry leaf with sprayer, do not drip and be advisable to squirt) mulberry leaf (water) group in contrast;
With the mulberry leaf that do not spray (blank) group in contrast;
Using new fresh mulberry leaf as object of reference;
Spray after 6,12,18 and 24 hours, the mulberry leaf percentage of water loss of experimental group 1～3, contrast (water) group, contrast (blank) group is as shown in table 1 below;
Table 1, the impact of mulberry leaf preservation agent for storing of the present invention on mulberry leaf percentage of water loss
Remarks explanation: the % in above-mentioned table 1 represents mulberry leaf percentage of water loss; Index represents mulberry leaf percentage of water loss and contrasts the percentage of (blank) mulberry leaf percentage of water loss.
According to table 1, we learn: spray after 6,12,18 and 24 hours, index of the present invention is respectively 40.79～47.67%, 42.70～46.17%, 45.52～49.42% and 48.82～52.99%, than contrast (blank), have reduced over half.
Mulberry leaf with sprinkling after 6,12,18 and 24 hours were raised for 5 ages and are played silkworm until put on an arrow, every group each 30 of test silkworms, and experimental cultivar is rich 1 * 54A.
Silkworm is played to the cocoon impact of rate of silkworm 5 ages as shown in table 2; Impact on silkworm cocoon quality is as shown in table 3.
Table 2, mulberry leaf preservation agent for storing of the present invention play the cocoon impact of rate of silkworm 5 ages to silkworm
Table 3, the impact of mulberry leaf preservation agent for storing of the present invention on silkworm cocoon quality
According to table 2 and table 3, we learn: the edible mulberry leaf of antistaling agent after 6,12,18 and 24 hours that spray, rise for 5 ages silkworm cocoon rate and new fresh mulberry leaf raise substantially similar, apparently higher than spraying distilled water and untreated blank.The mulberry leaf that use this antistaling agent to spray after 12hr are raised silkworm, and silk cocoon amount improves 8.9%～13.1%, and cocoon shell weight increases by 5.7%～11.8%; The mulberry leaf that spray after 24hr are raised silkworm, and silk cocoon amount improves 6.9～10.8%, and cocoon shell weight increases by 5.2%～12.4%.
The use of comparative example 1-1, cancellation norfloxacin hydrochloride, all the other are equal to embodiment 3.
Comparative example 1-2, norfloxacin hydrochloride is modified to Soluble norfloxacin, all the other are equal to embodiment 3.
Comparative example 1-3, the consumption of norfloxacin hydrochloride is modified to 1500mg by 1000mg, all the other are equal to embodiment 3
Comparative example 1-4, the consumption of norfloxacin hydrochloride is modified to 400mg by 1000mg, all the other are equal to embodiment 3.
Above-mentioned 4 comparative examples are detected according to the method described in above-mentioned experiment 1, and the conclusion of final gained is as follows:
The investigation result of table 4, comparative example 1 series
The use of comparative example 2-1, cancellation NaCl, all the other are with embodiment 3.
Comparative example 2-2, the consumption of NaCl is modified to 10g by 5g, all the other are with embodiment 3.
Comparative example 2-3, the consumption of NaCl is modified to 1g by 5g, all the other are with embodiment 3.
Above-mentioned 3 comparative examples are detected according to the method described in above-mentioned experiment 1, and the conclusion of final gained is as follows:
The investigation result of table 5, comparative example 2 series
Comparative example 3-1, cancel ascorbic use, all the other are with embodiment 3.
Comparative example 3-2, ascorbic consumption is modified to 80g by 60g, all the other are with embodiment 3.
Comparative example 3-3, ascorbic consumption is modified to 30g by 60g, all the other are with embodiment 3.
Above-mentioned 3 comparative examples are detected according to the method described in above-mentioned experiment 1, and the conclusion of final gained is as follows:
The investigation result of table 6, comparative example 3 series
The use of comparative example 4-1, cancellation sucrose, all the other are with embodiment 3.
Comparative example 4-2, the consumption of sucrose is modified to 80g by 60g, all the other are with embodiment 3.
Comparative example 4-3, the consumption of sucrose is modified to 30g by 60g, all the other are with embodiment 3.
Above-mentioned 3 comparative examples are detected according to the method described in above-mentioned experiment 1, and the conclusion of final gained is as follows:
The investigation result of table 7, comparative example 4 series
Finally, it is also to be noted that, what more than enumerate is only several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, can also have many distortion.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention, all should think protection scope of the present invention.
Claims (3)
1. the mulberry leaf preservation agent for storing with silk cocoon production-increasing function, is characterized in that: described mulberry leaf preservation agent for storing is obtained by mixing according to the volume ratio of 1:1 by reagent A and reagent B;
Every L reagent A is grouped into by the one-tenth of following quality:
Vitamin C 40～60g, NaCl5g, norfloxacin hydrochloride 1000mg, shitosan 10～30g, acetic acid 30～50g, citric acid 20～40g, sucrose 40～60g, surplus is water;
Described reagent B is the aqueous solution that radioresistant bacterium D.radiodurans R1 cultivates thing; In the described aqueous solution, the mass content of radioresistant bacterium D.radiodurans R1 cultivation thing is 0.6%～1%.
2. the mulberry leaf preservation agent for storing with silk cocoon production-increasing function according to claim 1, is characterized in that: described radioresistant bacterium D.radiodurans R1 cultivates the preparation method of thing for comprising the following steps:
1), by preserving number, be the radioresistant bacterium D.radiodurans R1 bacterium liquid of ATCC NO.13939 by carrying out fermented and cultured in 5% inoculum concentration access defatted soybean culture medium, in 29.5～30.5 ℃, cultivate 58～62h;
2), by the fermented and cultured product of step 1) gained in the centrifugal 4.5～5.5min of 5000～6000rpm, remove supernatant, the radioresistant bacterium wet thallus of gained is paved into equably to the thickness of 0.25～0.35cm, at frequency 600MHz, under the condition of electric-field intensity 8000V/m, process 38～42 seconds, make clasmatosis, thereby obtain through broken radioresistant bacterium;
3), by described, through broken radioresistant bacterium, be placed in material disc, put into and in cold wind drier, carry out cold air drying, chilling temperature is controlled at 20～25 ℃, wind speed 1.5～2.0m/s, relative humidity 10～15%, be dried to water content 6～8%, obtain radioresistant bacterium D.radiodurans R1 and cultivate thing.
3. the mulberry leaf preservation agent for storing with silk cocoon production-increasing function according to claim 1, is characterized in that:
Described defatted soybean culture medium consists of the following composition: fineness is 60-80 object defatted soy flour 20g, glucose 2g, and sodium nitrate 2g, yeast extract 1g, adding distil water is to 1000mL.
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|---|---|---|---|
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| CN201310732797.XA CN103719642B (en) | 2013-12-26 | 2013-12-26 | Mulberry leaf storage preservative with effect of increasing silkworm cocoon yield |
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| CN1362030A (en) * | 2001-01-02 | 2002-08-07 | 成都天友发展有限公司 | Mulberry preserving agent |
| CN1362029A (en) * | 2001-01-02 | 2002-08-07 | 成都天友发展有限公司 | Production process of dewatered mulberry leaf |
| CN101986848A (en) * | 2009-08-03 | 2011-03-23 | 刘艳梅 | Novel preservative and preparation method thereof |
| CN102626183A (en) * | 2012-04-01 | 2012-08-08 | 浙江大学 | Feed additive for improving output and quality of silkworm cocoons and preparation method thereof |
| CN102626184A (en) * | 2012-04-03 | 2012-08-08 | 浙江大学 | Feed additive for improving laying yield of female silkworm moths and preparation method thereof |
-
2013
- 2013-12-26 CN CN201310732797.XA patent/CN103719642B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1362030A (en) * | 2001-01-02 | 2002-08-07 | 成都天友发展有限公司 | Mulberry preserving agent |
| CN1362029A (en) * | 2001-01-02 | 2002-08-07 | 成都天友发展有限公司 | Production process of dewatered mulberry leaf |
| CN101986848A (en) * | 2009-08-03 | 2011-03-23 | 刘艳梅 | Novel preservative and preparation method thereof |
| CN102626183A (en) * | 2012-04-01 | 2012-08-08 | 浙江大学 | Feed additive for improving output and quality of silkworm cocoons and preparation method thereof |
| CN102626184A (en) * | 2012-04-03 | 2012-08-08 | 浙江大学 | Feed additive for improving laying yield of female silkworm moths and preparation method thereof |
Non-Patent Citations (2)
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| 李东光: "《150种保鲜剂配方与制作》", 30 September 2011, 化学工业出版社 * |
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