CN103705593A - Alpha-glucosidase inhibitor extracted from potentilla bifurca L - Google Patents
Alpha-glucosidase inhibitor extracted from potentilla bifurca L Download PDFInfo
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- CN103705593A CN103705593A CN201410016761.6A CN201410016761A CN103705593A CN 103705593 A CN103705593 A CN 103705593A CN 201410016761 A CN201410016761 A CN 201410016761A CN 103705593 A CN103705593 A CN 103705593A
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Abstract
The invention relates to the technical field of traditional Chinese medicines, and in particular relates to an alpha-glucosidase inhibitor extracted from potentilla bifurca L. The alpha-glucosidase inhibitor is extracted from the whole potentilla bifurca L and is prepared by the following steps: smashing the whole potentilla bifurca L taken as a raw material; enabling the smashed potentilla bifurca L to pass a sieve with 24 meshes; performing reflux extraction thrice by using 70% of ethyl alcohol, the volume of which is six times as that of the potentilla bifurca L; combining filtrates and performing vacuum concentration on the filtrates; then dispersing the filtrates into water; extracting the mixture by using petroleum ether, the volume of which is four times as that of the mixture, so as to obtain an ethyl acetate extract; performing vacuum drying on the ethyl acetate extract, so as to obtain the alpha-glucosidase inhibitor. Thus, a complete technical scheme for extracting the alpha-glucosidase inhibitor from the potentilla bifurca L is provided. The alpha-glucosidase inhibitor prepared according to the technical scheme can be used for efficiently inhibiting the activity of an alpha-glucosidase so as to decrease the postprandial blood sugar of a II-type diabetes patient.
Description
Technical field
The present invention relates to technical field of Chinese medicines, specifically a kind of alpha-glucosidase inhibitor extracting and preparation method thereof from short life two decomposite leaf Herba Potentillae Chinensis.
Background technology
Short life two decomposite leaf Herba Potentillae Chinensis (
potentilla bifurca var. humiliorr), the short and small paving of plant is loose, and scape is long not as good as 7 centimetres, the common 3-5 couple of lobule, and rare 6 pairs, many full edge, even top 2 is split, and spends normal Dan Sheng, the flowering fruit bearing stage 5-10 month.Originate in the ground such as Qinghai, Gansu, Ningxia, Xinjiang, Sichuan and Tibet, be born in height above sea level 1800-4000 rice patana, river shoal sand ground and arid grassland.According to the literature, the chemical composition of potentilla plants mainly comprises flavonoid, terpenoid, tannin class and phenols.Pharmacological research shows that this platymiscium has anti-diabetic, blood fat reducing, antibacterial, antiviral, the effect such as protects the liver, but the chemical composition of short life two decomposite leaf Herba Potentillae Chinensis and pharmacological action have no report in studying at home and abroad.The present invention studies discovery first, and the extract of short life two decomposite leaf Herba Potentillae Chinensis has efficient alpha-glucosaccharase enzyme inhibition, and can effectively reduce post-prandial glycemia.Alpha-glucosidase (α-glucosidase, AG) is the disaccharidase hydrolytic enzyme that a class is distributed in small intestine epithelium mucosa microvillose membrane surface, comprises maltase, isomaltase, saccharase and trehalase etc.This enzyme can make the disaccharidase of taking in human body change the monosaccharide that is easy to absorption of human body into, and catabolism of carbohydrate is had to important function.Alpha-glucosidase inhibitor is a kind of medicine of novel treatment diabetes, by with AG, competitive inhibition occurring, reaches the object that reduces type 2 diabetes mellitus post-prandial glycemia.
Summary of the invention
Alpha-glucosidase inhibitor in the present invention extracts and obtains from the short life two decomposite leaf Herba Potentillae Chinensis of Qinghai-Tibet pure natural plant, is a kind of potential drug of novel reduction type 2 diabetes mellitus post-prandial glycemia.
The invention provides a kind of scheme of extracting alpha-glucosidase inhibitor from short life two decomposite leaf Herba Potentillae Chinensis herbs, it is achieved by following technology: a kind of alpha-glucosidase inhibitor extracting from short life two decomposite leaf Herba Potentillae Chinensis herbs, and described inhibitor is to extract and obtain from short life two decomposite leaf Herba Potentillae Chinensis herbs; This inhibitor is made as follows: the short life two decomposite leaf Herba Potentillae Chinensis herbs of take are raw material, cross after crushed 24 mesh sieves, with 70% alcohol reflux of 6 times of volumes 3 times, each 1 h, sucking filtration, merge 3 times filtrate, after filtrate vacuum concentration, be scattered in water, adopt the petroleum ether extraction layering of 4 times of volumes, gained water layer is carried out to layering with the ethyl acetate extraction of 4 times of volumes, obtain acetic acid ethyl acetate extract, through vacuum drying, obtain short life two decomposite leaf potentilla chinensis general flavones, this total flavones is alpha-glucosidase inhibitor.
The alpha-glucosidase inhibitor that the present invention extracts from short life two decomposite leaf Herba Potentillae Chinensis is compared and is had following beneficial effect with existing medicine: this alpha-glucosidase inhibitor adopts alpha-glucosidase active determination in vitro method, enzyme inhibition activity is measured, and result shows: at sample concentration, be 1.953 * 10
-2during mg/mL, the suppression ratio of this alpha-glucosidase inhibitor is 99.78%; Half-inhibition concentration IC
50be 3.370 * 10
-3mg/mL.And existing medicine acarbose half-inhibition concentration IC
50be 0.626 mg/mL.The suppression ratio of the alpha-glucosidase inhibitor in the present invention is high, is to extract and obtain from the short life two decomposite leaf Herba Potentillae Chinensis of natural plants, and extraction process is easy, safety, for preparing the drug provision foundation of novel reduction type 2 diabetes mellitus post-prandial glycemia.
This invention provides a kind of potential medicine for reducing type 2 diabetes mellitus post-prandial glycemia.Its key technology is the screening of alpha-glucosidase inhibitor and the extracting method of medicine, the method advantages of simple, and easy operating, finishing screen is selected the active component that alpha-glucosidase is had to remarkable inhibition.The short life two decomposite leaf potentilla chinensis general flavones that the present invention filters out in addition can be used for take the research and development of the product that Inhibiting α-glucosidase is object, especially can be used for reducing the exploitation of type 2 diabetes mellitus post-prandial glycemia medicine, have industrialization meaning.
The short life two decomposite leaf Herba Potentillae Chinensis of raw material used in the present invention pick up from Qinghai-Tibet Platean, have wild, free of contamination feature; The present invention provides a set of perfect technical scheme for the extraction of alpha-glucosidase inhibitor in short life two decomposite leaf Herba Potentillae Chinensis, Inhibiting α-glucosidase is active efficiently to adopt the alpha-glucosidase inhibitor that this scheme extracts, and reduces type 2 diabetes mellitus post-prandial glycemia.
Accompanying drawing explanation
Fig. 1 is a kind of method technological process Fig. 1 that extracts alpha-glucosidase inhibitor from short life two decomposite leaf Herba Potentillae Chinensis of the present invention
The specific embodiment
Below in conjunction with drawings and Examples, a kind of preparation method, technical scheme of extracting alpha-glucosidase inhibitor from short life two decomposite leaf Herba Potentillae Chinensis of the present invention is further described.
As shown in Figure 1, a kind of alpha-glucosidase inhibitor extracting from short life two decomposite leaf Herba Potentillae Chinensis herbs, described inhibitor is to extract and make from short life two decomposite leaf Herba Potentillae Chinensis herbs; Described inhibitor is made as follows:
The short life two decomposite leaf Herba Potentillae Chinensis herbs of take are raw material, cross after crushed 24 mesh sieves, with 70% alcohol reflux of 6 times of volumes 3 times, each 1 h, sucking filtration, merge 3 times filtrate, after filtrate vacuum concentration, be scattered in water, adopt the petroleum ether extraction layering of 4 times of volumes, gained water layer is carried out to layering with the ethyl acetate extraction of 4 times of volumes, obtain acetic acid ethyl acetate extract, through vacuum drying, obtain short life two decomposite leaf potentilla chinensis general flavones, this total flavones is alpha-glucosidase inhibitor.
Described alpha-glucosidase inhibitor adopts alpha-glucosidase active determination in vitro method, carries out the mensuration of enzyme inhibition activity, and result shows: at sample concentration, be 1.953 * 10
-2during mg/mL, the suppression ratio of this alpha-glucosidase inhibitor is 99.78%; Half-inhibition concentration IC
50be 3.370 * 10
-3mg/mL.
Embodiment 1.
(1) main technical schemes:
Raw material and adjuvant: short life two decomposite leaf Herba Potentillae Chinensis, distilled water, ethanol, petroleum ether, ethyl acetate, other.
Capital equipment: disintegrating machine, vacuum pump, rotary evaporator, condensation cycle water pump etc.
The extraction scheme of a kind of alpha-glucosidase inhibitor extracting from short life two decomposite leaf Herba Potentillae Chinensis herbs of the present invention is as follows: get after short life two decomposite leaf Herba Potentillae Chinensis herbs are pulverized and cross 24 mesh sieves, take 200g, with 70% alcohol reflux of 6 times of volumes 3 times, each 1 h, sucking filtration, merge 3 times filtrate, after filtrate vacuum concentration, be scattered in water, adopt the petroleum ether extraction layering of 4 times of volumes, gained water layer is carried out to layering with the ethyl acetate extraction of 4 times of volumes, obtain acetic acid ethyl acetate extract, through vacuum drying, obtain short life two decomposite leaf potentilla chinensis general flavones, this total flavones is alpha-glucosidase inhibitor.This alpha-glucosidase inhibitor adopts alpha-glucosidase active determination in vitro method, carries out the mensuration of enzyme inhibition activity, and result shows: at sample concentration, be 1.953 * 10
-2during mg/mL, the suppression ratio of this alpha-glucosidase inhibitor is 99.78%; Half-inhibition concentration IC
50be 3.370 * 10
-3mg/mL.The suppression ratio of the alpha-glucosidase inhibitor in the present invention is high, is to extract and obtain from the short life two decomposite leaf Herba Potentillae Chinensis of natural plants, and extraction process is easy, safety, for preparing the drug provision foundation of novel reduction type 2 diabetes mellitus post-prandial glycemia.This invention provides a kind of potential medicine for reducing type 2 diabetes mellitus post-prandial glycemia.Its key technology is the screening of alpha-glucosidase inhibitor and the extracting method of medicine, the method advantages of simple, and easy operating, finishing screen is selected the active component that alpha-glucosidase is had to remarkable inhibition.The short life two decomposite leaf potentilla chinensis general flavones that the present invention filters out in addition can be used for take the research and development of the product that Inhibiting α-glucosidase is object, especially can be used for reducing the exploitation of type 2 diabetes mellitus post-prandial glycemia medicine, have industrialization meaning.
Claims (2)
1. the alpha-glucosidase inhibitor extracting from short life two decomposite leaf Herba Potentillae Chinensis, is characterized in that: described inhibitor is to extract and obtain from short life two decomposite leaf Herba Potentillae Chinensis herbs; This inhibitor is made as follows: the short life two decomposite leaf Herba Potentillae Chinensis herbs of take are raw material, cross after crushed 24 mesh sieves, with 70% alcohol reflux of 6 times of volumes 3 times, each 1 h, sucking filtration, merge 3 times filtrate, after filtrate vacuum concentration, be scattered in water, adopt the petroleum ether extraction layering of 4 times of volumes, gained water layer is carried out to layering with the ethyl acetate extraction of 4 times of volumes, obtain acetic acid ethyl acetate extract, through vacuum drying, obtain short life two decomposite leaf potentilla chinensis general flavones, this total flavones is alpha-glucosidase inhibitor.
2. the alpha-glucosidase inhibitor extracting from short life two decomposite leaf Herba Potentillae Chinensis, is characterized in that: described alpha-glucosidase inhibitor adopts alpha-glucosidase active determination in vitro method, carries out the mensuration of enzyme inhibition activity; Result shows: at sample concentration, be 1.953 * 10
-2during mg/mL, the suppression ratio of this alpha-glucosidase inhibitor is 99.78%; Half-inhibition concentration IC
50be 3.370 * 10
-3mg/mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410016761.6A CN103705593A (en) | 2014-01-15 | 2014-01-15 | Alpha-glucosidase inhibitor extracted from potentilla bifurca L |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410016761.6A CN103705593A (en) | 2014-01-15 | 2014-01-15 | Alpha-glucosidase inhibitor extracted from potentilla bifurca L |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103977099A (en) * | 2014-05-29 | 2014-08-13 | 青海师范大学 | Latent medicament for treating type 2 diabetes mellitus |
| CN110256512A (en) * | 2019-07-09 | 2019-09-20 | 青海师范大学 | A kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca |
-
2014
- 2014-01-15 CN CN201410016761.6A patent/CN103705593A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| 李瑾等: "委陵菜提取物对L6肌细胞糖代谢影响的研究", 《食品科学》 * |
| 陆璐等: "委陵菜降血糖成分的机理研究", 《食品科学》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103977099A (en) * | 2014-05-29 | 2014-08-13 | 青海师范大学 | Latent medicament for treating type 2 diabetes mellitus |
| CN110256512A (en) * | 2019-07-09 | 2019-09-20 | 青海师范大学 | A kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca |
| CN110256512B (en) * | 2019-07-09 | 2022-07-15 | 青海师范大学 | Alpha-glucosidase inhibitor extracted from Potentilla chinensis Franch |
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Application publication date: 20140409 |
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