CN110256512A - A kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca - Google Patents
A kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca Download PDFInfo
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Abstract
The present invention relates to technical field of traditional Chinese medicines, specifically a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, by being obtained after extraction in short raw Potentilla bifurca and extraction, column chromatography for separation and glucoside inhibiting activity verifying.Alpha-glucosidase restrainer in the application is extracted from a kind of relatively more extensive short raw Potentilla bifurca of natural plants of Qinghai-Tibet Platean distribution, it is a kind of monomeric compound, purity is high, inhibiting rate is high, extraction process is easy, safe, time discovery headed by the alpha-glucosaccharase enzyme inhibition activity of the alpha-glucosidase restrainer of acquisition, and inhibitory effect is excellent, foundation can be provided to prepare novel hypoglycemic activity drug, which can be the drug of reduction diabetes postprandial blood sugar.
Description
Technical field
The present invention relates to technical field of traditional Chinese medicines, specifically a kind of phlorose extracted from short raw Potentilla bifurca
Glycosides enzyme inhibitor and its extracting method and application.
Background technique
Diabetes are one of World Health diseases of 21 century most serious, bring great harm to the mankind.α-glucosides
Enzyme inhibitor class drug is currently used hypoglycemic medicine, but such drug synthesized has more side effect, causes to human body
Different degrees of damage.The hypoglycemic activity ingredient small toxicity of separation is extracted from natural plants, while there are also preferable hypoglycemics to make
With.Separation hypoglycemic activity ingredient is extracted from natural plants to be facilitated developing new drug and promotes the development and utilization of natural plants.
Short raw Potentilla bifurca (Potentilla bifurca var.Humilior R) be rosaceae (Rosaceae)
Potentilla (Potentilla) plant, the short and small paving of plant is scattered, and scape is long not as good as 7 centimetres, usual 3-5 pairs of leaflet, dilute 6
Right, more full edges, even top 2 is split, Hua Chang Dan Sheng, the flowering fruit bearing stage 5-10 month.Originate in Qinghai, Gansu, Ningxia, Xinjiang, Sichuan and
1800-4000 m patana of height above sea level, river shoal sand ground and aridity grass land are born in the ground such as Tibet." national Chinese herbal medicine compilation " note
Its sweet property, micro-pungent, cool is carried, hemostasis, stop dysentery are cured mainly, for functional uterine bleeding, the excessive, dysentery of postpartum haemorrhage etc..Modern times grind
Study carefully and show that the chemical component of this platymiscium is related to flavones, triterpene, tannin, organic acid etc., pharmacological research shows that this platymiscium is main
There are hypoglycemic, antibacterial, antitumor, anti-oxidant, anticancer, anti-cerebral ischemia damnification, antiallergy, antiviral, liver protection, treatment sclerotin to dredge
The effects of pine, analgesia.Short raw Potentilla bifurca monomeric compound has time discovery headed by higher alpha-glucosaccharase enzyme inhibition activity.
Alpha-glucosidase (α-glucosidase, AG) is a kind of disaccharide hydrolysis for being distributed in small intestine epithelium mucous membrane microvillose membrane surface
Enzyme, including maltose, isomaltase, invertase and trehalase etc..The enzyme can be such that the disaccharide for taking in human body is changed into easily
In the monosaccharide of absorption of human body, play a significant role to catabolism of carbohydrate.Alpha-glucosidase restrainer is a kind of reduces after the meal
The drug of the treatment diabetes of blood glucose achievees the purpose that reduce postprandial blood sugar by the way that Reverse transcriptase occurs with AG.
There are problems for the prior art: most common alpha-glucosidase restrainer hypoglycemic medicine acarbose can be effective
Diabetes are controlled, reduce blood glucose, but its target user is limited, is not suitable for meat, the high protein food rich in fat such as eggs is
The crowd of staple food, at the same because its small intestinal degradation and absorb slowly, extended residence time, the glycolysis through enteric bacteria and produce gas
Increase, abdominal distension, abdominal pain and diarrhea etc. can be caused, and the price of acarbose is higher, is not suitable for compared with low-income groups.Other
Hypoglycemic medicine also has different degrees of side effect and social concern.
Summary of the invention
The technical problem to be solved in the present invention is that providing the alpha-glucosidase suppression extracted from short raw Potentilla bifurca
Preparation.
In order to solve the above technical problems, the present invention adopts the following technical scheme:
A kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, the inhibitor are 1-O- coumaric acyl-
β-D-Glucose (1-O-coumaroyl- β-D-glucose).The alpha-glucosidase restrainer uses alpha-glucosidase body
Outer activity determination method carries out the measurement of enzyme inhibition activity, as the result is shown: when sample concentration is 2.5mg/mL, 1-O- tonka-bean
The alpha-glucosaccharase enzyme inhibition rate of acyl-beta-D-Glucose (1-O-coumaroyl- β-D-glucose) is 99.41%, IC50For
0.0019mg/mL。
A kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, the inhibitor are as follows: myricetin
(myricetin), which uses alpha-glucosidase active determination in vitro method, carries out enzyme and inhibits to live
Property measurement, as the result is shown: when sample concentration is 1.25mg/mL, the alpha-glucosidase of myricetin (myricetin) inhibits
Rate is 90.67%, IC50For 0.0561mg/mL.
A kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, the inhibitor are as follows: silver linden glycoside
(tiliroside), which uses alpha-glucosidase active determination in vitro method, carries out enzyme inhibition
Active measurement, as the result is shown: when sample concentration is 1.25mg/mL, the alpha-glucosidase of silver linden glycoside (tiliroside)
Inhibiting rate is 87.38%, IC50For 0.1807mg/mL.
A kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, the inhibitor are as follows: catechin
((+)-catechin), the alpha-glucosidase restrainer use alpha-glucosidase active determination in vitro method, carry out enzyme suppression
Active measurement is made, as the result is shown: when sample concentration is 1.25mg/mL, the phlorose of catechin ((+)-catechin)
Glycosides enzyme inhibition rate is 96.52%, IC50For 0.0652mg/mL.
A kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, the inhibitor are as follows: Quercetin -3-
O- (6''-O- trans--to hydroxyl cinnamyl)-β-D-Glucose glycosides (quercetin-3-O- (6''-O-trans-p-
Coumaroyl)-β-D-glucoside), which uses alpha-glucosidase active determination in vitro side
Method carries out the measurement of enzyme inhibition activity, as the result is shown: when sample concentration is 1.25mg/mL, (6''-O- is anti-by Quercetin -3-O-
Formula-is to hydroxyl cinnamyl)-β-D-Glucose glycosides (quercetin-3-O- (6''-O-trans-p-coumaroyl)-β-D-
Glucoside alpha-glucosaccharase enzyme inhibition rate) is 92.06%, IC50For 0.0498mg/mL.
A kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, the inhibitor are as follows: Quercetin
(quercetin), which uses alpha-glucosidase active determination in vitro method, carries out enzyme and inhibits to live
Property measurement, as the result is shown: when sample concentration is 1.25mg/mL, the alpha-glucosidase of Quercetin (quercetin) inhibits
Rate is 88.16%, IC50For 0.2095mg/mL.
A kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, the inhibitor are as follows: rugosin A
Methyl esters (methyl ester of rugosin A), the alpha-glucosidase restrainer use alpha-glucosidase external activity
Measuring method carries out the measurement of enzyme inhibition activity, as the result is shown: when sample concentration is 2.5mg/mL, rugosin A methyl esters
The alpha-glucosaccharase enzyme inhibition rate of (methyl ester of rugosin A) is 97.77%, IC50For 0.0004mg/mL.
A kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, the inhibitor are as follows: (-)-table
Theine ((-)-epicatechin), the alpha-glucosidase restrainer use alpha-glucosidase active determination in vitro method, into
The measurement of row enzyme inhibition activity, as the result is shown: when sample concentration is 2.5mg/mL, (-)-epicatechin ((-)-
Epicatechin alpha-glucosaccharase enzyme inhibition rate) is 103.29%, IC50For 0.2087mg/mL.
A kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, the inhibitor are as follows: 3,5,7- tri-
Hydroxyl -4'- methoxy flavone (3,5,7-trihydroxy-4'-methoxyflavone), the alpha-glucosidase restrainer are adopted
With alpha-glucosidase active determination in vitro method, the measurement of enzyme inhibition activity is carried out, as the result is shown: being in sample concentration
When 0.625mg/mL, 3,5,7- trihydroxy -4'- methoxy flavones (3,5,7-trihydroxy-4'-methoxyflavone)
Alpha-glucosaccharase enzyme inhibition rate is 91.98%, IC50For 0.0019mg/mL.
Detailed description of the invention
Fig. 1 is alpha-glucosidase restrainer extraction process flow chart；
Fig. 2 is 1-O- coumaric acyl-β-D-Glucose (1-O-coumaroyl- β-D-glucose) structural schematic diagram；
Fig. 3 is myricetin (myricetin) structural schematic diagram；
Fig. 4 is silver linden glycoside (tiliroside) structural schematic diagram；
Fig. 5 is catechin ((+)-catechin) structural schematic diagram；
Fig. 6 is Quercetin -3-O- (6''-O- trans--to hydroxyl cinnamyl)-β-D-Glucose glycosides (quercetin-3-O-
(6''-O-trans-p-coumaroyl)-β-D-glucoside) structural schematic diagram；
Fig. 7 is Quercetin (quercetin) structural schematic diagram；
Fig. 8 is rugosin A methyl esters (methyl ester of rugosin A) structural schematic diagram；
Fig. 9 is (-)-epicatechin ((-)-epicatechin) structural schematic diagram；
Figure 10 is 3,5,7- trihydroxy -4'- methoxy flavone (3,5,7-trihydroxy-4'-methoxyflavone) structure
Schematic diagram.
Specific implementation method
Application method and device in the following embodiments of the invention patent are unless otherwise specified conventional method and device；It is used
Equipment, reagent are the conventional equipment and reagent of Reagent Company's purchase.For the purposes, technical schemes and advantages for making the invention patent
It is clearer, the specific embodiment of the invention patent is described in detail combined with specific embodiments below.These are preferred real
The example for applying mode is illustrated in a particular embodiment.
Here, it should also be noted that, in order to avoid having obscured the technical side of the invention patent because of unnecessary details
Case illustrate only the technical solution and/or processing step closely related with the scheme of patent according to the present invention in embodiment,
And the little other details of relationship are omitted.
Embodiment 1
The present embodiment provides a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, the inhibitor is 1-
O- coumaric acyl-β-D-Glucose (1-O-coumaroyl- β-D-glucose), chemical structure is as shown in Fig. 2.α-the Portugal
Polyglycoside enzyme inhibitor uses alpha-glucosidase active determination in vitro method, carries out the measurement of enzyme inhibition activity, as the result is shown:
When sample concentration is 2.5mg/mL, the α-of 1-O- coumaric acyl-β-D-Glucose (1-O-coumaroyl- β-D-glucose)
Glucosidase inhibitor rate is 99.41%, IC50For 0.0019mg/mL.
Embodiment 2
The present embodiment provides a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, the inhibitor are as follows:
Myricetin (myricetin), the alpha-glucosidase restrainer use alpha-glucosidase active determination in vitro method, carry out enzyme
The measurement of inhibitory activity, as the result is shown: when sample concentration is 1.25mg/mL, the alpha-glucosaccharase of myricetin (myricetin)
Enzyme inhibition rate is 90.67%, IC50For 0.0561mg/mL, chemical structure is as shown in Fig. 3.
Embodiment 3
The present embodiment provides a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, the inhibitor are as follows:
Silver linden glycoside (tiliroside), the alpha-glucosidase restrainer use alpha-glucosidase active determination in vitro method, carry out
The measurement of enzyme inhibition activity, as the result is shown: when sample concentration is 1.25mg/mL, α-grape of silver linden glycoside (tiliroside)
Glucosides enzyme inhibition rate is 87.38%, IC50For 0.1807mg/mL, chemical structure is as shown in Fig. 4.
Embodiment 4
The present embodiment provides a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, the inhibitor are as follows:
Catechin ((+)-catechin), the alpha-glucosidase restrainer use alpha-glucosidase active determination in vitro method, into
The measurement of row enzyme inhibition activity, as the result is shown: when sample concentration is 1.25mg/mL, the α-of catechin ((+)-catechin)
Glucosidase inhibitor rate is 96.52%, IC50For 0.0652mg/mL, chemical structure is as shown in Fig. 5.
Embodiment 5
The present embodiment provides a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, the inhibitor are as follows:
Quercetin -3-O- (6''-O- trans--to hydroxyl cinnamyl)-β-D-Glucose glycosides (quercetin-3-O- (6''-O-
Trans-p-coumaroyl)-β-D-glucoside), which is lived in vitro using alpha-glucosidase
Property measuring method, carry out the measurement of enzyme inhibition activity, as the result is shown: sample concentration be 1.25mg/mL when, Quercetin -3-O-
(6''-O- trans--to hydroxyl cinnamyl)-β-D-Glucose glycosides (quercetin-3-O- (6''-O-trans-p-
Coumaroyl)-β-D-glucoside) alpha-glucosaccharase enzyme inhibition rate be 92.06%, IC50For 0.0.0498mg/mL, change
It is as shown in Fig. 6 to learn structure.
Embodiment 6
The present embodiment provides a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, the inhibitor are as follows:
Quercetin (quercetin), the alpha-glucosidase restrainer use alpha-glucosidase active determination in vitro method, carry out enzyme
The measurement of inhibitory activity, as the result is shown: when sample concentration is 1.25mg/mL, the alpha-glucosaccharase of Quercetin (quercetin)
Enzyme inhibition rate is 88.16%, IC50For 0.2095mg/mL, chemical structure is as shown in Fig. 7.
Embodiment 7
The present embodiment provides a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, the inhibitor are as follows:
Rugosin A methyl esters (methyl ester of rugosin A), the alpha-glucosidase restrainer use alpha-glucosidase
Active determination in vitro method carries out the measurement of enzyme inhibition activity, as the result is shown: when sample concentration is 2.5mg/mL, gauffer bacterium
The alpha-glucosaccharase enzyme inhibition rate of plain A methyl esters (methyl ester of rugosin A) is 97.77%, IC50For 0.0004mg/
ML, chemical structure are as shown in Fig. 8.
Embodiment 8
The present embodiment provides a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, the inhibitor are as follows:
(-)-epicatechin ((-)-epicatechin), the alpha-glucosidase restrainer are surveyed using alpha-glucosidase external activity
Determine method, carries out the measurement of enzyme inhibition activity, as the result is shown: when sample concentration is 2.5mg/mL, (-)-epicatechin ((-)-
Epicatechin alpha-glucosaccharase enzyme inhibition rate) is 103.29%, IC50For 0.2087mg/mL, chemical structure such as attached drawing 9
It is shown.
Embodiment 9
The present embodiment provides a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, the inhibitor are as follows:
3,5,7- trihydroxy -4'- methoxy flavones (3,5,7-trihydroxy-4'-methoxyflavone), the alpha-glucosidase
Inhibitor uses alpha-glucosidase active determination in vitro method, carries out the measurement of enzyme inhibition activity, as the result is shown: dense in sample
When degree is 0.625mg/mL, 3,5,7- trihydroxy -4'- methoxy flavones (3,5,7-trihydroxy-4'-
Methoxyflavone alpha-glucosaccharase enzyme inhibition rate) is 91.98%, IC50For 0.0019mg/mL, chemical structure such as attached drawing
Shown in 10.
Embodiment 10
The present embodiment provides a kind of extracting methods of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, such as attached
Shown in Fig. 1, include the following steps:
(1) Preparatory work of experiment: raw material and auxiliary material: short raw Potentilla bifurca, silica gel plate (thin layer use), silica gel (column chromatography use),
Sephadex LH-20(25-100 μm), MCI CHP-20P(75-100 μm), solvent be (benzene: Ethyl formate: formic acid) three-phase
Solution；Ethanol solution of sulfuric acid that color developing agent is 10% and 1% ferric trichloride ethanol solution (wherein color developing agent is the pure preparation of analysis),
Distilled water, methanol, petroleum ether, ethyl acetate, other.Capital equipment: 600MHz Nuclear Magnetic Resonance, Auto Spec-3000 matter
Spectrometer, R1002 Rotary Evaporators, SHZ-D(III) circulating water type vacuum pump, ARA520 electronic analytical balance, ALC-210.3 electronics
The automatic receiving instrument of assay balance, BS-100A, BCD-215KA Haier refrigerator, Newstyle HPLC, disintegrating machine, vacuum pump, rotation
Evaporator, condensation cycle water pump, D101 resin column, Sephadex LH-20, MCI-gel CHP-20P, RP-18, HPLC.
(2) it extracts and extracts: a kind of alpha-glucosidase restrainer extracted from short two decomposite leaf potentilla chinensis herb of life, institute
The inhibitor stated is to extract to obtain from short two decomposite leaf potentilla chinensis herb of life；The inhibitor is made as follows: taking short life
Two decomposite leaf potentilla chinensis herbs crush, with 95% methanol, 65 DEG C of refluxing extractions, after extracting solution concentration and recovery methanol medicinal extract.Medicinal extract
It is extracted respectively with petroleum ether and ethyl acetate according to polarity size, obtains petroleum ether part, ethyl acetate extract and water layer and mention
Take position；
(3) it column chromatography for separation: is further extracted after taking ethyl acetate extract to dissolve dispersion with water with ether, obtains ether layer, water
Layer；Gained water layer, with the elution of 0%-30%-60%-80%-100% methanol aqueous solution, obtains water elution object, 30% methanol with D101 column
Eluate, 60% methanol eluate, 80% methanol eluate, 100% methanol eluate；After taking ethyl acetate extract ether to extract
80% D101 methanol eluate of water layer is chromatographed through Sephadex LH-20 column, with every 10% increasing concen-trations of water-methanol (1:0-0:1)
Gradient elution is carried out, 7 parts is obtained, 07 part is taken to chromatograph through MCI column, with every 10% increasing concen-trations of water-methanol (1:0-0:1)
20 parts (1.3 g) is obtained after carrying out gradient elution, is chromatographed through Sephadex LH-20 column, it is every with water-methanol (1:0-0:1)
10% increasing concen-trations obtain part 21(87 mg after carrying out gradient elution), then chromatographed using MCI column, with water-methanol (1:0-0:1)
Every 10% increasing concen-trations obtain compound 1-O- coumaric acyl-β-D-Glucose (1-O-coumaroyl- after carrying out gradient elution
β-D-glucose), as alpha-glucosidase restrainer.Other 8 kinds of compound extracting methods are as shown in Fig. 1.
(4) glucoside inhibiting activity is verified: the alpha-glucosidase restrainer is surveyed using alpha-glucosidase external activity
Determine method, carries out the measurement of enzyme inhibition activity.Specific assay method is as follows:
AG inhibitor screening model principle: AG catalyzing hydrolysis PNPG generates paranitrophenol (PNP), and PNP has under 400 nm wavelength
There is absorption peak, measure the quantum of output of PNG, calculates short raw Potentilla bifurca monomeric compound to the inhibitory activity of AG.
The inhibitory activity of enzyme measures full-automatic microplate reader, and reaction carries out in non-disconnectable 96 orifice plate.Reaction system is 200
μ L: in 125 μ L phosphate buffers and 8.92 × 10-3It is 1.953 × 10 that concentration is added in the 25 μ L of PNPG of mol/L-2 mg/
37 DEG C of 20 min of warm bath after the 25 μ L of AG of the sample 25 μ L and 0.05 U/mL of mL, using full-automatic microplate reader in 400 nm waves
Long lower measurement absorbance, and replace enzyme solution to make blank control with distilled water.Each sample does 3 repetitions and tests, and takes its average value.
The definition of AG unit of activity: under the conditions of 37 DEG C of 6.8 pH, interior enzyme hydrolysis PNPG discharges the amount (OD of PNG per minute
Value).Inhibitor unit of activity definition: the amount of inhibitor needed for reducing by 1 enzyme activity unit under the same conditions.
In order to eliminate the influence of sample and substrate PNPG to measurement result, need to measure the background absorption of sample and substrate
Value.Sample and substrate is replaced to be corrected with the phosphate buffer of 0.05 mol/mL.
Inhibiting rate (%)=[(A1-A3)-(A2-A4)]/(A1-A3)
A1: original enzyme activity；A2: the enzyme activity after inhibitor is added；A3:PNPG background；A4: sample background
As the result is shown: when sample concentration is 2.5mg/mL, 1-O- coumaric acyl-β-D-Glucose (1-O-coumaroyl- β-
D-glucose alpha-glucosaccharase enzyme inhibition rate) is 99.41%, IC50For 0.0019 mg/mL.
When sample concentration is 1.25mg/mL, the alpha-glucosaccharase enzyme inhibition rate of myricetin (myricetin) is
90.67%, IC50For 0.0561mg/mL.
When sample concentration is 1.25mg/mL, the alpha-glucosaccharase enzyme inhibition rate of silver linden glycoside (tiliroside) is
87.38%, IC50For 0.1807mg/mL.
When sample concentration is 1.25mg/mL, the alpha-glucosaccharase enzyme inhibition rate of catechin ((+)-catechin) is
96.52%, IC50For 0.0652 mg/mL.
When sample concentration is 1.25mg/mL, the Portugal-β-D- Quercetin -3-O- (6''-O- trans--to hydroxyl cinnamyl)
The alpha-glucosidase of polyglycoside (quercetin-3-O- (6''-O-trans-p-coumaroyl)-β-D-glucoside) presses down
Rate processed is 92.06%, IC50For 0.0498 mg/mL.
When sample concentration is 1.25mg/mL, the alpha-glucosaccharase enzyme inhibition rate of Quercetin (quercetin) is
88.16%, IC50For 0.2095 mg/mL.
When sample concentration is 2.5mg/mL, α-Portugal of rugosin A methyl esters (methyl ester of rugosin A)
Polyglycoside enzyme inhibition rate is 97.77%, IC50For 0.0004 mg/mL.
When sample concentration is 2.5mg/mL, the alpha-glucosidase suppression of (-)-epicatechin ((-)-epicatechin)
Rate processed is 103.29%, IC50For 0.2087 mg/mL.
When sample concentration is 0.625mg/mL, 3,5,7- trihydroxy -4'- methoxy flavones (3,5,7-trihydroxy-
Alpha-glucosaccharase enzyme inhibition rate 4'-methoxyflavone) is 91.98%, IC50For 0.0019 mg/mL.
The utility model has the advantages that
Alpha-glucosidase restrainer inhibiting rate of the present invention is high, is extracted from natural plants, and extraction process is easy, safe, is obtained
The alpha-glucosidase restrainer excellent effect obtained, the hypoglycemic activity drug to prepare novel provide foundation, can be sugared to reduce
It urinates sick postprandial blood sugar and a kind of potential drug is provided.Its key technology be alpha-glucosidase inhibitor medicament extracting method and
Screening, method is reasonable, and finishing screen, which is selected, the monomeric compound for significantly inhibiting effect to alpha-glucosidase.In addition present invention sieve
The inhibitor selected can be used for the research and development of the product for the purpose of inhibiting alpha-glucosidase, be used especially for reducing diabetes meal
The exploitation of hypoglycemic medicament afterwards has industrialization meaning.
Alpha-glucosidase restrainer in the application is short from a kind of relatively more extensive natural plants of Qinghai-Tibet Platean distribution
It is extracted in raw Potentilla bifurca, is a kind of monomeric compound, purity is high, inhibiting rate is high, and extraction process is easy, safe, obtains
Alpha-glucosidase restrainer alpha-glucosaccharase enzyme inhibition activity headed by time discovery, and inhibitory effect is excellent, can be preparation
Novel hypoglycemic activity drug provides foundation, which can be the drug of reduction diabetes postprandial blood sugar.
The above is only the specific embodiment of the application, it is noted that for the ordinary skill people of the art
For member, under the premise of not departing from the application principle, several improvements and modifications can also be made, these improvements and modifications are also answered
It is considered as the protection scope of the application.
Claims (10)
1. a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, which is characterized in that the inhibitor is
1-O- coumaric acyl-β-D-Glucose (1-O-coumaroyl- β-D-glucose).
2. a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, which is characterized in that the inhibitor is
Myricetin (myricetin).
3. a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, which is characterized in that the inhibitor is
Silver linden glycoside (tiliroside).
4. a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, which is characterized in that the inhibitor is
Catechin ((+)-catechin).
5. a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, which is characterized in that the inhibitor is
Quercetin -3-O- (6''-O- trans--to hydroxyl cinnamyl)-β-D-Glucose glycosides (quercetin-3-O- (6''-O-
Trans-p-coumaroyl)-β-D-glucoside).
6. a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, which is characterized in that the inhibitor is
Quercetin (quercetin).
7. a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, which is characterized in that the inhibitor is
Rugosin A methyl esters (methyl ester of rugosin A).
8. a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, which is characterized in that the inhibitor is
(-)-epicatechin ((-)-epicatechin).
9. a kind of alpha-glucosidase restrainer extracted from short raw Potentilla bifurca, which is characterized in that the inhibitor is
3,5,7- trihydroxy -4'- methoxy flavone (3,5,7-trihydroxy-4'-methoxyflavone).
10. a kind of alpha-glucosidase restrainer extracting method extracted from short raw Potentilla bifurca, which is characterized in that including
Following steps:
(1) Preparatory work of experiment；
(2) it extracts and extracts: a kind of alpha-glucosidase restrainer extracted from short two decomposite leaf potentilla chinensis herb of life, it is described
Inhibitor is to extract to obtain from short two decomposite leaf potentilla chinensis herb of life；The inhibitor is made as follows: short life two being taken to split
Leaf potentilla chinensis herb crushes, with 95% methanol, 65 DEG C of refluxing extractions, after extracting solution concentration and recovery methanol medicinal extract；Medicinal extract according to
Polarity size is extracted with petroleum ether and ethyl acetate respectively, obtains petroleum ether part, ethyl acetate extract and water layer extraction unit
Position；
(3) it column chromatography for separation: is further extracted after taking ethyl acetate extract to dissolve dispersion with water with ether, obtains ether layer, water
Layer；Gained water layer, with the elution of 0%-30%-60%-80%-100% methanol aqueous solution, obtains water elution object, 30% methanol with D101 column
Eluate, 60% methanol eluate, 80% methanol eluate, 100% methanol eluate；After taking ethyl acetate extract ether to extract
80% D101 methanol eluate of water layer is chromatographed through Sephadex LH-20 column, with every 10% increasing concen-trations of water-methanol (1:0-0:1)
Gradient elution is carried out, 7 parts is obtained, 07 part is taken to chromatograph through MCI column, with every 10% increasing concen-trations of water-methanol (1:0-0:1)
20 parts (1.3 g) is obtained after carrying out gradient elution, is chromatographed through Sephadex LH-20 column, it is every with water-methanol (1:0-0:1)
10% increasing concen-trations obtain part 21(87 mg after carrying out gradient elution), then chromatographed using MCI column, with water-methanol (1:0-0:1)
Every 10% increasing concen-trations obtain compound 1-O- coumaric acyl-β-D-Glucose (1-O-coumaroyl- after carrying out gradient elution
β-D-glucose)；
(4) glucoside inhibiting activity is verified.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111067949A (en) * | 2019-12-27 | 2020-04-28 | 延边大学 | Potentilla tormentosa total flavone effective part with lipopexia inhibiting effect, and preparation method and application thereof |
| CN114504612A (en) * | 2020-11-17 | 2022-05-17 | 中国医学科学院药物研究所 | Application of potentilla anserine extract in preparation of medicine for treating gastric ulcer |
| CN115177663A (en) * | 2022-08-12 | 2022-10-14 | 青海师范大学 | Preparation method and application of Potentilla chinensis extract |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103705593A (en) * | 2014-01-15 | 2014-04-09 | 青海师范大学 | Alpha-glucosidase inhibitor extracted from potentilla bifurca L |
| KR20160118072A (en) * | 2015-04-01 | 2016-10-11 | 대구가톨릭대학교산학협력단 | A composition comprising compounds isolated from Smilax china for preventing or treating metabolic disorder |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103705593A (en) * | 2014-01-15 | 2014-04-09 | 青海师范大学 | Alpha-glucosidase inhibitor extracted from potentilla bifurca L |
| KR20160118072A (en) * | 2015-04-01 | 2016-10-11 | 대구가톨릭대학교산학협력단 | A composition comprising compounds isolated from Smilax china for preventing or treating metabolic disorder |
Non-Patent Citations (9)
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111067949A (en) * | 2019-12-27 | 2020-04-28 | 延边大学 | Potentilla tormentosa total flavone effective part with lipopexia inhibiting effect, and preparation method and application thereof |
| CN111067949B (en) * | 2019-12-27 | 2022-08-12 | 延边大学 | Potentilla tormentosa total flavone effective part with lipopexia inhibiting effect, and preparation method and application thereof |
| CN114504612A (en) * | 2020-11-17 | 2022-05-17 | 中国医学科学院药物研究所 | Application of potentilla anserine extract in preparation of medicine for treating gastric ulcer |
| CN115177663A (en) * | 2022-08-12 | 2022-10-14 | 青海师范大学 | Preparation method and application of Potentilla chinensis extract |
| CN115177663B (en) * | 2022-08-12 | 2023-07-21 | 青海师范大学 | Preparation method and application of herba Potentilla chinensis extract |
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