CN103704529B - A kind of Broiler chicks feed addictive and preparation thereof, using method - Google Patents
A kind of Broiler chicks feed addictive and preparation thereof, using method Download PDFInfo
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- CN103704529B CN103704529B CN201310741106.2A CN201310741106A CN103704529B CN 103704529 B CN103704529 B CN 103704529B CN 201310741106 A CN201310741106 A CN 201310741106A CN 103704529 B CN103704529 B CN 103704529B
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Abstract
The invention belongs to field of feed additive technology, be specifically related to a kind of Broiler chicks feed addictive and preparation thereof, using method.This additive is made up of the raw material of following weight percentage: beta-xylanase 8-12%, bacillus licheniformis 1-3%, yeast cell wall 25-35%, montmorillonite 2 5-35%, and surplus is wheat bran.Feed addictive of the present invention can eliminate the antagonism of ANFs in feed, improve the digestibility of nutriment, play and strengthen animal body profitable strain quantity, stimulating animal generation nonspecific immune response, raising and adjustment animal cell immunity and the physiological function such as humoral immunity level, control disease, effectively can improve speed of weight increment and the feed conversion rate of Broiler chicks.
Description
Technical field
The invention belongs to field of feed additive technology, be specifically related to a kind of Broiler chicks feed addictive and preparation thereof, using method.
Background technology
The development that China's meat type chicken spends more than 20 year already, with the advantage of high efficiency, low cost, develops rapidly and becomes the industry that in Chinese farming and animal husbandry, industrialization level is the highest.Along with the raising of people's living standard, in meals there is profound change in consumption of meat structure, take chicken as the positive cumulative year after year of plain boiled pork consumption proportion of representative.But the broiler production of China also exists the sustainable development that many problems govern Chicken industry, because epidemic disease and chicken meat product drug residue exceed standard the event that China Broiler is implemented to forbid occurred repeatedly.By improving the immunity function of animal, reduce the generation of disease, thus reduce the use of feed and clinical veterinary drug, improving the security of livestock products, is the research direction that government always supports.Use the general principle of disease-resistant nutritive, by feed approach, add useful animal health, the disease-resistant feed additive formula with the resistance against diseases of plant extracts and the raising such as probiotics, the acidulant animal strengthening animal immunizing power ensures that animal health is significant.
Summary of the invention
The object of the present invention is to provide a kind of Broiler chicks feed addictive and preparation thereof, using method.
The present invention is by the following technical solutions:
A kind of Broiler chicks feed addictive, be made up of the raw material of following weight percentage: beta-xylanase 8-12%, bacillus licheniformis 1-3%, yeast cell wall 25-35%, montmorillonite 2 5-35%, surplus is wheat bran.
Further preferably, be made up of the raw material of following weight percentage: β zytase 10%, bacillus licheniformis 2%, yeast cell wall 30%, montmorillonite 2 5%, surplus is wheat bran.
The enzyme 5000-200000u/g alive of described beta-xylanase; Viable bacteria total content 2 × 10 in bacillus licheniformis
10cFU/g; Beta glucan content 20-30wt%, D-manna oligosacchride>=20wt% in described yeast cell wall, protein≤35wt%, moisture content≤6wt%; Montmorillonite is nano montmorillonite (purity is not less than 96%); Described wheat bran is secondary wheat bran, wherein moisture content≤13.5wt%, crude protein>=13.0wt%, crude fibre <10.0wt%, coarse ash <6.0wt%; Acidulant selects feed to allow the acidulant used, preferred liquid organic acidulant, total acid>=50%, pH value≤2.50.
When described Broiler chicks feed addictive uses simultaneously when drinking water with the use of acidulant, the addition of acidulant in water is 1kg/ ton water.
Prepare the method for Broiler chicks feed addictive, get each raw material in proportion, by each raw material blending.Also can granulate after mixing.
Broiler chicks feed addictive of the present invention can add in formula feed of broilers according to the ratio of 1-1.5w%, also can add in premix or concentrate feed according to a certain percentage by Broiler chicks feed addictive of the present invention, according to premix and concentrate feed, the addition in mixed feed converts concrete addition.
In feed addictive use procedure of the present invention, need with the use of acidulant in drinking-water, the addition of acidulant is 1kg/ ton water, freely drinks water.
The outstanding advantages of feed addictive of the present invention is: be grouped into by the one-tenth of useful animals health, have and have no side effect, and preparation method is simple, the advantage such as easy to use, use this disease-resistant feed additive formula can mycotoxin in adsorption feed, eliminate the antagonism of ANFs in feed, improve the digestibility of nutriment, play and strengthen animal body profitable strain quantity, stimulating animal produces nonspecific immune response, improve and regulate animal cell immunity and humoral immunity level, control the physiological functions such as disease, effectively can improve speed of weight increment and the feed conversion rate of Broiler chicks.
Detailed description of the invention
Embodiment 1
Broiler chicks feed addictive, is made up of the raw material of following weight percentage: beta-xylanase 10%, bacillus licheniformis 2%, yeast cell wall 30%, montmorillonite 2 5%, and surplus is wheat bran.
Embodiment 2
Broiler chicks feed addictive, is made up of the raw material of following weight percentage: beta-xylanase 12%, bacillus licheniformis 1%, yeast cell wall 35%, montmorillonite 30%, and surplus is wheat bran.
Embodiment 3
Broiler chicks feed addictive, is made up of the raw material of following weight percentage: beta-xylanase 8%, bacillus licheniformis 3%, yeast cell wall 25%, montmorillonite 35%, and surplus is wheat bran.
Each raw material is got in proportion time prepared by embodiment 1-3, by each raw material blending.
Effect experimental
Add in Broiler chicks feed by the feed addictive of embodiment 1 by the 1w% amount of mixed feed weight, on probation on fast large-scale white plumage Broiler chicks, effect is better.
Experimental design
Select 96 1 age in days AA
+healthy Broiler chicks, be divided into 2 process at random, each process 6 repetition, each repetition 8, raise in cages for 3 layers in units of repeating, to feed respectively basal diet+Bacitracin Zinc (control group) and basal diet+1% disease-resistant feed additive formula (processed group), in drinking-water, add ingenious acid (Germany continue Man produce) with the amount of 1kg/ ton water simultaneously.Diet Formula and trophic level are in table 1.Test chicken free choice feeding and drinking-water, 7 ages in days newly prop up Combined vaccine collunarium eye droppings, 14 age in days bursa of farbricius drinking-water immunity, and 19 age in days bursa of farbricius drinking-water immunity, 26 ages in days newly prop up the immunity of Combined vaccine drinking-water.Experimental period is 42 days, and result of the test is as follows:
One, test chicken daily ration composition and trophic level
Table 1 test chicken daily ration composition and trophic level
1) vitamin premix provides Vitaminpremixprovidedforperkilogramofdiet:VA12500IU for every kilogram of daily ration; VD
32500IU; VE18.75mg; VK
32.65mg; VB
12mg; VB
26mg; VB
62mg; VB
120.025mg; Biotin biotin0.0325mg; Folic acid folicacid1.25mg; Pantothenic acid pantothenicacid12mg; Nicotinic acid niacin50mg.
2) microelement pre-mix material provides Mineralpremixprovidedforperkilogramofdiet:I0.35mg for every kilogram of daily ration; Se0.15mg; Zn75mg; Cu8mg; Fe80mg; Mn100mg.
Two, on the impact of production performance
Table 2 is on the impact of meat chicken production performance
Note: in table, same row shoulder indicates different alphabetical person and represents significant difference (P<0.05).
Table 2 result shows, the processed group applying feed addictive of the present invention, compared with control group, can improve 42 age in days Broiler chicks daily gains 5.31%; Reduce feed intake 2.73%; Improve food conversion ratio 8.93%.
Three, on the impact of broiler chicken immune performance index
Table 3 immune organ relative weight (%) (n=6)
Note: in table, same row shoulder indicates different alphabetical person and represents significant difference (P<0.05).
Table 4 total serum protein, albumin, globulin (n=6)
Note: in table, same row shoulder indicates different alphabetical person and represents significant difference (P<0.05).
Table 5 PBLC conversion ratio (n=6)
Note: in table, same row shoulder indicates different alphabetical person and represents significant difference (P<0.05).
Spleen, thymus gland, the bursa of farbricius are the Main Immune Organs of bird, are the sources of humoral immunity and cellular immunity cell, and their developmental condition can reflect the function status of immunocyte and the immune level of body indirectly.The increase of immune organ weight is the performance of growing, and the raising of index then means that immune system maturation is very fast.
Serum globulins content is the important indicator of the non-specific humoral immune reaction of reflection body.This test shows: interpolation embodiment 1 feed addictive processed group 21 age in days and 42 age in days serum globulins content are significantly higher than control group, improves the nonspecific immune reaction of body after showing to add feed addictive of the present invention.
Lymphocyte transformation test is a kind of method of vitro detection T, bone-marrow-derived lymphocyte function, lymphocyte transformation rate is the important indicator evaluating cellular immune function, LPS can induce propagation and the conversion of bone-marrow-derived lymphocyte, and the mitogen that ConA is inducer T lymphocyte propagation and transforms.
Table 3,4,5 results show, apply feed addictive of the present invention, broiler chicken immune performance can be strengthened, increase immune organ weight, improve humoral immunity of organism and cellular immune level, processed group, compared with control group, can improve thymus gland, spleen, the bursal index of 21 age in days 42 age in days Broiler chicks; Improve 21 ages in days, 42 age in days Broiler chicks total serum proteins and globulin content and ConA and LPS SI.
Four, on the impact of Broiler chicks alimentary canal shape and Microflora
Table 6 is on the impact (n=6) of broiler chicken alimentary canal pH value
Note: in table, same row shoulder indicates different alphabetical person and represents significant difference (P<0.05).
Alimentary canal content pH value reduces, and is conducive to the growth of intestinal beneficial microbes, and suppress harmful bacteria growth, table 6 result shows, applies the processed group of feed addictive of the present invention, can reduce flesh Stomach duodenum, ileum and cecal content pH value.
Table 7 is on the impact (n=6) of broiler chicken Digestive tract length
Note: in table, same row shoulder indicates different alphabetical person and represents significant difference (P<0.05).
Table 8 is on the impact (n=6) of broiler chicken alimentary canal weight
Note: in table, same row shoulder indicates different alphabetical person and represents significant difference (P<0.05).
Digestive organs and alimentary canal are the places that nutrient digestion absorbs, and gastral length and weight can reflect gastral development degree, are directly proportional to digesting and assimilating of nutriment.Table 7,8 results show, apply the processed group of feed addictive of the present invention, significantly can affect jejunum, duodenum, ileum, caecum length, have the trend increasing glandular stomach, flesh Stomach duodenum, ileum, caecum weight.
Table 9 is on the impact (n=6) of 42 Day-old Broiler Chickens jejunum forms
Process | Villus length, μm | Crypt depth, μm | Fine hair/crypts, μm/μm |
Control group | 988.96±24.37 | 156.21±5.93 | 6.33±0.43 |
Processed group | 1022.45±24.76 | 151.92±5.10 | 6.71±0.27 |
Note: in table, same row shoulder indicates different alphabetical person and represents significant difference (P<0.05).
Intestinal villus is the main portions that Animal nutrition material absorbs, shorten gradually with moving after intestinal segment, intestinal mucosa surface significantly expands absorption area by generation fold and intensive fine hair, and the microvillus of the columnar epithelial cell of intestinal villus makes again absorption area increase hundreds of times simultaneously.Therefore, when fine hair shortens, enterocyte number reduces, and also just reduces the abilities of digestive and absorption of nutriment.Crypt depth reflects the production rate of enterocyte.Height of naps and Crypt depth reflect the absorption function state of small intestine, with the resorption of the ratio of villus length/Crypt depth then concentrated expression small intestine.Table 9 result shows, processed group, compared with control group, after adding feed addictive of the present invention, improves the villus length of jejunum, reduces Crypt depth.
Table 10 is on the impact (n=6) of 42 Day-old Broiler Chickens jejunum Microfloras
Bacillus acidi lactici log10cuf/g (fresh sample) | Escherichia coli log10cuf/g (fresh sample) | |
Control group | 6.46±0.39 b | 4.80±0.41 |
Processed group | 7.53±0.33 a | 4.07±0.13 |
Note: in table, same row shoulder indicates different alphabetical person and represents significant difference (P<0.05).
Escherichia coli, Bacillus acidi lactici are the normal flora of animal intestinal, and Bacillus acidi lactici is the important beneficial bacterium of enteron aisle, ensures animal health with other flora, and regulating the ecological balance in animal body, is indispensable bacterium.Some bacterial strain colibacillary is pathogenic pathogen, often causes Broiler chicks diarrhea and various inflammation, causes serious economic loss.Table 10 result shows, compared with control group, the processed group of adding feed addictive of the present invention does not add the quantity that improve Bacillus acidi lactici in broiler chicken Jejunal chyme, reduces colibacillary quantity.
five, conclusion
Disease-resistant feed additive formula provided by the invention, complex acid agent uses simultaneously, compared with interpolation antibiotic group, meat chicken production performance and immune performance can be improved, improve broiler chicken alimentary canal shape, improve alimentary canal profitable strain quantity and reduce harmful bacteria quantity, substitute antibiotics Be very effective in broiler production.
Claims (3)
1. a Broiler chicks feed addictive, is characterized in that, is made up of the raw material of following weight percentage: beta-xylanase 10%, bacillus licheniformis 2%, yeast cell wall 30%, montmorillonite 2 5%, and surplus is wheat bran;
When described Broiler chicks feed addictive uses simultaneously when drinking water with the use of acidulant, the addition of acidulant in water is 1kg/ ton water;
The enzyme 5000-200000u/g alive of described beta-xylanase; Viable bacteria total content 2 × 10 in bacillus licheniformis
10cFU/g; Beta glucan content 20-30wt%, D-manna oligosacchride>=20wt% in described yeast cell wall, protein≤35wt%, moisture content≤6wt%; Montmorillonite is nano montmorillonite, purity>=96%; Described wheat bran is secondary wheat bran, wherein moisture content≤13.5wt%, crude protein>=13.0wt%, crude fibre <10.0wt%, coarse ash <6.0wt%; Liquid organic acid agent selected by acidulant, total acid>=50%, pH value≤2.50.
2. prepare the method for Broiler chicks feed addictive described in claim 1, it is characterized in that, get each raw material in proportion, by each raw material blending.
3. the using method of Broiler chicks feed addictive described in claim 1, is characterized in that: when feed addictive uses simultaneously when drinking water with the use of acidulant, the addition of acidulant in water is 1kg/ ton water.
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CN104957372A (en) * | 2015-06-08 | 2015-10-07 | 亳州师范高等专科学校 | Compound feed additive and preparation method thereof |
CN105995147B (en) * | 2016-05-26 | 2019-06-07 | 河南省农业科学院畜牧兽医研究所 | A kind of Broiler chicks nonreactive diet feed additive premix and its application method |
CN106071305A (en) * | 2016-06-15 | 2016-11-09 | 环山集团有限公司 | A kind of reduce feedstuff broiler chicken batch just and production method |
CN107772195A (en) * | 2016-08-27 | 2018-03-09 | 瑞普(天津)生物药业有限公司 | One kind poultry poultry fodder takes off mould dose and preparation method thereof |
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DE10105347A1 (en) * | 2001-02-05 | 2002-08-22 | Nutrinova Gmbh | Sorbic acid preparation containing probiotics as a feed additive in livestock rearing |
CN101690544B (en) * | 2009-09-29 | 2012-08-29 | 北京伟嘉人生物技术有限公司 | Liquid organic acid acidifying agent |
CN102511686B (en) * | 2011-12-23 | 2013-10-23 | 山东六和农牧科技园有限公司 | Green broiler chicken feed additive and its preparation method |
CN103243028B (en) * | 2012-02-02 | 2015-11-25 | 安琪酵母股份有限公司 | Yeast cells wall and method for making, fodder additives and method for making thereof and feed |
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