CN103695505B - A kind of technique preparing antibacterial peptide - Google Patents
A kind of technique preparing antibacterial peptide Download PDFInfo
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- CN103695505B CN103695505B CN201310597282.3A CN201310597282A CN103695505B CN 103695505 B CN103695505 B CN 103695505B CN 201310597282 A CN201310597282 A CN 201310597282A CN 103695505 B CN103695505 B CN 103695505B
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Abstract
The present invention proposes a kind of technique preparing antibacterial peptide, comprise step: by the Bacillus licheniformis of 2 ~ 4 weight parts, the bacillus cereus of 0.5 ~ 1.5 weight part, the bacillus natto of 1 ~ 2 weight part mixes; By the above-mentioned material mixed 36 ~ 39 DEG C of bottom fermentations 6 ~ 10 hours, obtain laying in seed; By deposit seed obtained above in fermentor tank, 36 ~ 39 DEG C of bottom fermentations 27 ~ 37 hours; By deposit seed complete for above-mentioned fermentation through filter press; By spray-dried for deposit seed complete for above-mentioned filtration, obtain described antibacterial peptide; Three strain fermentations that present invention process is produced are to Hemolytic streptococcus, streptococcus pneumoniae to faecalis, diphtheria corynebacterium, anthrax bacillus, gram-negative facultatively anacrobic rod, Clostridium perfringens, tetanus bacillus clostridium difficile, propionibacterium, Eubacterium, lactobacilluss etc. are all effective, and separately this technique can shorten the production cycle about 1/3rd, reduce use cost 60%.
Description
Technical field
The present invention relates to biological technical field, refer to a kind of technique preparing antibacterial peptide especially.
Background technology
There is narrow antimicrobial spectrum in existing market antibacterial peptide product, antibacterial range single culture fermentation to Hemolytic streptococcus, streptococcus pneumoniae has effect to faecalis, invalid to other, and the production cycle is long, and production cost is high.
Summary of the invention
The present invention proposes a kind of technique preparing antibacterial peptide, and solve antibacterial peptide product narrow antimicrobial spectrum in prior art, the production cycle is long, the problem that production cost is high.
Technical scheme of the present invention is achieved in that a kind of technique preparing antibacterial peptide, comprises step:
1) by the Bacillus licheniformis of 2 ~ 4 weight parts, the bacillus cereus of 0.5 ~ 1.5 weight part, the bacillus natto of 1 ~ 2 weight part mixes;
2) by the above-mentioned material mixed anaerobically fermenting 6 ~ 10 hours at 36 ~ 39 DEG C, obtain laying in seed;
3) by deposit seed obtained above in fermentor tank, anaerobically fermenting 27 ~ 37 hours at 36 ~ 39 DEG C;
4) by deposit seed complete for above-mentioned fermentation through filter press;
5) the deposit seed by complete for above-mentioned filtration is spray-dried, obtains described antibacterial peptide.
As preferred technical scheme, by the Bacillus licheniformis of 3 weight parts, the bacillus cereus of 1 weight part, the bacillus natto of 1.5 weight parts mixes.
As preferred technical scheme, described step 2) in fermentation time be 7 ~ 9 hours.
As preferred technical scheme, described fermentation time is 8 hours.
As preferred technical scheme, in described step 3), fermentation time is 30 ~ 34 hours.
As preferred technical scheme, described fermentation time is 32 hours.
The plate-and-frame filter press used through filter press in described step 4) forms one group of filter chamber by the filter plate be alternately arranged and filter frame, there is groove on the surface of filter plate, its protruding parts is in order to supporting filter cloth, the corner of filter frame and filter plate there is through hole, form complete passage after assembling, suspension, washing water and extraction filtrate can be passed into.Plate, each jug rest in frame both sides are on crossbeam, and by hold-down gear pressure strip, frame, the filter cloth between plate, frame plays gasket seal.By charging pump, suspension is pressed into filter chamber, filter cloth forms filter residue, until be full of filter chamber; Filtrate, through filter cloth and along filter plate trench flow to sheet frame corner passage, is concentrated and is discharged.Filter complete, cleaning can be passed into and wash water washing filter residue.After washing, sometimes also pass into pressurized air, remove remaining washings.Open pressure filter removal filter residue subsequently, cleaning filter cloth, again pressure strip, frame, start subsequent work circulation.
As preferred technical scheme, in described step 5), spray-dired drying temperature is 80 ~ 95 DEG C.
Owing to have employed technique scheme, a kind of technique preparing antibacterial peptide, comprises step: 1) by the Bacillus licheniformis of 2 ~ 4 weight parts, the bacillus cereus of 0.5 ~ 1.5 weight part, and the bacillus natto of 1 ~ 2 weight part mixes; 2) by the above-mentioned material mixed anaerobically fermenting 6 ~ 10 hours at 36 ~ 39 DEG C, obtain laying in seed; 3) by deposit seed obtained above in fermentor tank, anaerobically fermenting 27 ~ 37 hours at 36 ~ 39 DEG C; 4) by deposit seed complete for above-mentioned fermentation through filter press; 5) the deposit seed by complete for above-mentioned filtration is spray-dried, obtains described antibacterial peptide; Three strain fermentations that present invention process is produced are to Hemolytic streptococcus, streptococcus pneumoniae to faecalis, diphtheria corynebacterium, anthrax bacillus, gram-negative facultatively anacrobic rod, Clostridium perfringens, tetanus bacillus clostridium difficile, propionibacterium, Eubacterium, lactobacilluss etc. are all effective, and separately this technique can shorten the production cycle about 1/3rd, reduce use cost 60%.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the process flow diagram of preparation method of the present invention.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
As shown in Figure 1, a kind of technique preparing antibacterial peptide, comprises step:
1) by the Bacillus licheniformis of 2 ~ 4 weight parts, the bacillus cereus of 0.5 ~ 1.5 weight part, the bacillus natto of 1 ~ 2 weight part mixes;
2) by the above-mentioned material mixed anaerobically fermenting 6 ~ 10 hours at 36 ~ 39 DEG C, obtain laying in seed;
3) by deposit seed obtained above in fermentor tank, anaerobically fermenting 27 ~ 37 hours at 36 ~ 39 DEG C;
4) by deposit seed complete for above-mentioned fermentation through filter press;
5) the deposit seed by complete for above-mentioned filtration is spray-dried, obtains described antibacterial peptide.
Described step 2) in fermentation time be 7 ~ 9 hours.
In described step 3), fermentation time is 30 ~ 34 hours.
The plate-and-frame filter press used through filter press in described step 4) forms one group of filter chamber by the filter plate be alternately arranged and filter frame, there is groove on the surface of filter plate, its protruding parts is in order to supporting filter cloth, the corner of filter frame and filter plate there is through hole, form complete passage after assembling, suspension, washing water and extraction filtrate can be passed into.Plate, each jug rest in frame both sides are on crossbeam, and by hold-down gear pressure strip, frame, the filter cloth between plate, frame plays gasket seal.By charging pump, suspension is pressed into filter chamber, filter cloth forms filter residue, until be full of filter chamber; Filtrate, through filter cloth and along filter plate trench flow to sheet frame corner passage, is concentrated and is discharged.Filter complete, cleaning can be passed into and wash water washing filter residue.After washing, sometimes also pass into pressurized air, remove remaining washings.Open pressure filter removal filter residue subsequently, cleaning filter cloth, again pressure strip, frame, start subsequent work circulation.
In described step 5), spray-dired drying temperature is 80 ~ 95 DEG C.
Embodiment one:
Prepare a technique for antibacterial peptide, comprise step:
1) by the Bacillus licheniformis of 2 weight parts, the bacillus cereus of 0.5 weight part, the bacillus natto of 1 weight part mixes;
2) by the above-mentioned material mixed anaerobically fermenting 6 hours at 36 DEG C, obtain laying in seed;
3) by deposit seed obtained above in fermentor tank, anaerobically fermenting 27 hours at 36 DEG C;
4) by deposit seed complete for above-mentioned fermentation through filter press;
5) the deposit seed by complete for above-mentioned filtration is spray-dried, obtains described antibacterial peptide.
The plate-and-frame filter press used through filter press in described step 4) forms one group of filter chamber by the filter plate be alternately arranged and filter frame, there is groove on the surface of filter plate, its protruding parts is in order to supporting filter cloth, the corner of filter frame and filter plate there is through hole, form complete passage after assembling, suspension, washing water and extraction filtrate can be passed into.Plate, each jug rest in frame both sides are on crossbeam, and by hold-down gear pressure strip, frame, the filter cloth between plate, frame plays gasket seal.By charging pump, suspension is pressed into filter chamber, filter cloth forms filter residue, until be full of filter chamber; Filtrate, through filter cloth and along filter plate trench flow to sheet frame corner passage, is concentrated and is discharged.Filter complete, cleaning can be passed into and wash water washing filter residue.After washing, sometimes also pass into pressurized air, remove remaining washings.Open pressure filter removal filter residue subsequently, cleaning filter cloth, again pressure strip, frame, start subsequent work circulation.
In described step 5), spray-dired drying temperature is 80 DEG C.
Embodiment two:
Prepare a technique for antibacterial peptide, comprise step:
1) by the Bacillus licheniformis of 3 weight parts, the bacillus cereus of 1 weight part, the bacillus natto of 1.5 weight parts mixes;
2) by the above-mentioned material mixed anaerobically fermenting 8 hours at 38 DEG C, obtain laying in seed;
3) by deposit seed obtained above in fermentor tank, anaerobically fermenting 32 hours at 38 DEG C;
4) by deposit seed complete for above-mentioned fermentation through filter press;
5) the deposit seed by complete for above-mentioned filtration is spray-dried, obtains described antibacterial peptide.
The plate-and-frame filter press used through filter press in described step 4) forms one group of filter chamber by the filter plate be alternately arranged and filter frame, there is groove on the surface of filter plate, its protruding parts is in order to supporting filter cloth, the corner of filter frame and filter plate there is through hole, form complete passage after assembling, suspension, washing water and extraction filtrate can be passed into.Plate, each jug rest in frame both sides are on crossbeam, and by hold-down gear pressure strip, frame, the filter cloth between plate, frame plays gasket seal.By charging pump, suspension is pressed into filter chamber, filter cloth forms filter residue, until be full of filter chamber; Filtrate, through filter cloth and along filter plate trench flow to sheet frame corner passage, is concentrated and is discharged.Filter complete, cleaning can be passed into and wash water washing filter residue.After washing, sometimes also pass into pressurized air, remove remaining washings.Open pressure filter removal filter residue subsequently, cleaning filter cloth, again pressure strip, frame, start subsequent work circulation.
In described step 5), spray-dired drying temperature is 90 DEG C.
Embodiment three:
Prepare a technique for antibacterial peptide, comprise step:
1) by the Bacillus licheniformis of 4 weight parts, the bacillus cereus of 1.5 weight parts, the bacillus natto of 2 weight parts mixes;
2) be anaerobically fermenting 10 hours by the above-mentioned material mixed at 39 DEG C, obtain laying in seed;
3) by deposit seed obtained above in fermentor tank, anaerobically fermenting 37 hours at 39 DEG C;
4) by deposit seed complete for above-mentioned fermentation through filter press;
5) the deposit seed by complete for above-mentioned filtration is spray-dried, obtains described antibacterial peptide.
The plate-and-frame filter press used through filter press in described step 4) forms one group of filter chamber by the filter plate be alternately arranged and filter frame, there is groove on the surface of filter plate, its protruding parts is in order to supporting filter cloth, the corner of filter frame and filter plate there is through hole, form complete passage after assembling, suspension, washing water and extraction filtrate can be passed into.Plate, each jug rest in frame both sides are on crossbeam, and by hold-down gear pressure strip, frame, the filter cloth between plate, frame plays gasket seal.By charging pump, suspension is pressed into filter chamber, filter cloth forms filter residue, until be full of filter chamber; Filtrate, through filter cloth and along filter plate trench flow to sheet frame corner passage, is concentrated and is discharged.Filter complete, cleaning can be passed into and wash water washing filter residue.After washing, sometimes also pass into pressurized air, remove remaining washings.Open pressure filter removal filter residue subsequently, cleaning filter cloth, again pressure strip, frame, start subsequent work circulation.
In described step 5), spray-dired drying temperature is 95 DEG C.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (7)
1. prepare a technique for antibacterial peptide, it is characterized in that, comprise step:
1) by the Bacillus licheniformis of 2 ~ 4 weight parts, the bacillus cereus of 0.5 ~ 1.5 weight part, the bacillus natto of 1 ~ 2 weight part mixes;
2) by the above-mentioned material mixed anaerobically fermenting 6 ~ 10 hours at 36 ~ 39 DEG C, obtain laying in seed;
3) by deposit seed obtained above in fermentor tank, anaerobically fermenting 27 ~ 37 hours at 36 ~ 39 DEG C;
4) by fermentation liquor filter press complete for above-mentioned fermentation;
5) filter press is obtained solid spray-dried, obtain described antibacterial peptide.
2. a kind of technique preparing antibacterial peptide as claimed in claim 1, it is characterized in that: by the Bacillus licheniformis of 3 weight parts, the bacillus cereus of 1 weight part, the bacillus natto of 1.5 weight parts mixes.
3. a kind of technique preparing antibacterial peptide as claimed in claim 1, is characterized in that: described step 2) in fermentation time be 7 ~ 9 hours.
4. a kind of technique preparing antibacterial peptide as claimed in claim 3, is characterized in that: described fermentation time is 8 hours.
5. a kind of technique preparing antibacterial peptide as claimed in claim 1, is characterized in that: described step 3) in fermentation time be 30 ~ 34 hours.
6. a kind of technique preparing antibacterial peptide as claimed in claim 5, is characterized in that: described fermentation time is 32 hours.
7. a kind of technique preparing antibacterial peptide as claimed in claim 1, is characterized in that: described step 5) in spray-dired drying temperature be 80 ~ 95 DEG C.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310597282.3A CN103695505B (en) | 2013-11-22 | 2013-11-22 | A kind of technique preparing antibacterial peptide |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310597282.3A CN103695505B (en) | 2013-11-22 | 2013-11-22 | A kind of technique preparing antibacterial peptide |
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| CN103695505A CN103695505A (en) | 2014-04-02 |
| CN103695505B true CN103695505B (en) | 2015-12-30 |
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| CN104498385B (en) * | 2014-11-17 | 2016-04-13 | 浙江大学 | High yield antibacterial peptide Bacillus licheniformis and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321182A (en) * | 2011-09-22 | 2012-01-18 | 广东海洋大学 | Composite antibiosis peptide and working method thereof that bacillus natto NT-6 bacterial strain is produced |
| CN102919514A (en) * | 2012-11-15 | 2013-02-13 | 江西一领药业有限公司 | Preparation method of feed protein peptide without probiotic bacteria activity resistance |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102321182A (en) * | 2011-09-22 | 2012-01-18 | 广东海洋大学 | Composite antibiosis peptide and working method thereof that bacillus natto NT-6 bacterial strain is produced |
| CN102919514A (en) * | 2012-11-15 | 2013-02-13 | 江西一领药业有限公司 | Preparation method of feed protein peptide without probiotic bacteria activity resistance |
Non-Patent Citations (1)
| Title |
|---|
| 地衣芽孢杆菌W10抗菌蛋白的分离纯化及其理化性质研究;纪兆林等;《植物病理学报》;20070615;第37卷(第03期);摘要，第260页左栏第1段 * |
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Granted publication date: 20151230 Termination date: 20171122 |