CN107286152A - A kind of extracting method of high-purity berberine - Google Patents
A kind of extracting method of high-purity berberine Download PDFInfo
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- CN107286152A CN107286152A CN201710715731.8A CN201710715731A CN107286152A CN 107286152 A CN107286152 A CN 107286152A CN 201710715731 A CN201710715731 A CN 201710715731A CN 107286152 A CN107286152 A CN 107286152A
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- film
- berberine
- permeate
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D455/00—Heterocyclic compounds containing quinolizine ring systems, e.g. emetine alkaloids, protoberberine; Alkylenedioxy derivatives of dibenzo [a, g] quinolizines, e.g. berberine
- C07D455/03—Heterocyclic compounds containing quinolizine ring systems, e.g. emetine alkaloids, protoberberine; Alkylenedioxy derivatives of dibenzo [a, g] quinolizines, e.g. berberine containing quinolizine ring systems directly condensed with at least one six-membered carbocyclic ring, e.g. protoberberine; Alkylenedioxy derivatives of dibenzo [a, g] quinolizines, e.g. berberine
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Nitrogen Condensed Heterocyclic Rings (AREA)
Abstract
The invention discloses a kind of extracting method of high-purity berberine, extraction step is as follows：A, the coptis is put into baking oven dried at a temperature of 50 70 DEG C, then stirred broken with machine, obtain Golden Thread；B, the Golden Thread for obtaining step A add cellulase solution, and 2-3 hours are incubated at a temperature of 45 60 DEG C；C, the solution for obtaining step B add ethanol, and heating is boiled, and with filtered through gauze, filter residue is washed twice with ethanol；D, ceramic film process, collect ceramic membrane permeate；E, seperation film carry out separating treatment, collect seperation film permeate；F, by seperation film permeate by concentrate film carry out concentration, collect concentration film permeate；G, concentration film dope is subjected to low-temperature vacuum drying, dries to powdered and take out, obtain finished product berberine.The present invention is a kind of extracting method of high-purity berberine, and berberine DNA purity is high, extracting cycle is short, and productivity ratio is high, energy consumption is low, pollution-free.
Description
Technical field
The invention belongs to technical field of membrane separation, and in particular to a kind of extracting method of high-purity berberine.
Background technology
Berberine is a kind of important alkaloid, is the Chinese medicine of China's application for a long time, it has significant bacteriostasis.It is yellow
Lian Suneng is to resisting pathogenic microbes, to various bacteria such as shigella dysenteriae, tubercle bacillus, pneumococcus, typhoid bacillus and diphtheria bar
Bacterium etc. has inhibitory action, wherein acting on most strong to shigella dysenteriae, is commonly used to the alimentary canal diseases such as treatment bacterial gastroenteritis, dysentery
Disease.Clinic is mainly used in treatment bacillary dysentery and enterogastritis, and its side effect is smaller.The extracting method operation of traditional berberine
Complexity, equipment is expensive, and DNA purity is not high, and yield is relatively low.
Plant cell wall is mainly made up of cellulose, hemicellulose and pectic substance, and cellulase is one kind to degrade
The protein of cellulose, therefore cell membrane can be removed using cellulase, when being applied among reality of cellulase can contract
Short extraction time, solvent usage amount is reduced, save energy resource, reduction production cost etc..
The content of the invention
It is an object of the invention to provide a kind of DNA purity is high, the cycle is short, high income high-purity berberine extraction
Method.
The technical scheme is that a kind of extracting method of high-purity berberine, including raw material stoving are crushed, add fiber
Plain enzyme solutions, extraction filtering, membrane filtration, separation, film concentration and dry series of steps, concrete operation step are as follows：
A, the coptis is put into baking oven dried at a temperature of 50-70 DEG C, then stir broken with machine；
B, the Golden Thread for obtaining step A add cellulase solution, and 2-3 hours are incubated at a temperature of 45-60 DEG C；
C, the comminution coptis for obtaining B add ethanol, and heating is boiled, and with filtered through gauze, filter residue is washed twice with ethanol；
D, under the high temperature conditions with ceramic film process, obtain ceramic membrane permeate and separation of ceramic film dope collected ceramic membrane and passed through
Liquid；
E, separating treatment carried out by seperation film, obtain seperation film permeate and seperation film dope, collect seperation film permeate；
F, by seperation film permeate by concentrate film carry out concentration, obtain concentration film concentrate and concentrate film permeate, receive
Collection concentration film concentrate；
G, concentration film dope is subjected to low-temperature vacuum drying, dries to powdered and take out, obtain finished product berberine.
It is preferred that, the weight/mass percentage composition of the cellulase solution in step B is 3%-5%.
It is preferred that, concentration of alcohol described in step C is 95%.
The present invention is a kind of extracting method of high-purity berberine, crushed by raw material stoving, add cellulase solution,
Extraction filtering, membrane filtration, separation, film concentration and drying process finally give the berberine finished product of high-purity, carry out sealing refrigeration
Storage.
Beneficial effects of the present invention：
The present invention is a kind of extracting method of high-purity berberine, is extracted by film, improves berberine DNA purity height, passes through fibre
The plain enzyme solutions of dimension, destroy cell membrane, accelerate extracting cycle, reduce the use of other solvents, energy consumption is low, pollution-free, the Huang of extraction
Lian Su is used as medicine can be to resisting pathogenic microbes, to various bacteria such as shigella dysenteriae, tubercle bacillus, pneumococcus, typhoid bacillus and white
Larynx bacillus etc. has inhibitory action, and side effect is very small.
Embodiment
For ease of skilled artisan understands that the present invention program, in conjunction with embodiment to technical solution of the present invention
It is further described in detail.
The present invention is a kind of extracting method of high-purity berberine, including raw material stoving crush, add cellulase solution,
Extract filtering, membrane filtration, separation, film concentration and dry series of steps, concrete operation step is as follows：
A, the coptis is put into baking oven dried at a temperature of 50-70 DEG C, then stir broken with machine；
B, the Golden Thread for obtaining step A add cellulase solution, and 2-3 hours are incubated at a temperature of 45-60 DEG C；
C, the comminution coptis for obtaining B add ethanol, and heating is boiled, and with filtered through gauze, filter residue is washed twice with ethanol；
D, under the high temperature conditions with ceramic film process, obtain ceramic membrane permeate and separation of ceramic film dope collected ceramic membrane and passed through
Liquid；
E, separating treatment carried out by seperation film, obtain seperation film permeate and seperation film dope, collect seperation film permeate；
F, by seperation film permeate by concentrate film carry out concentration, obtain concentration film concentrate and concentrate film permeate, receive
Collection concentration film concentrate；
G, concentration film dope is subjected to low-temperature vacuum drying, dries to powdered and take out, obtain finished product berberine.
The weight/mass percentage composition of cellulase solution in step B is 3%-5%.Concentration of alcohol described in step C is 95%.
The present invention program is invented above and is exemplarily described, it is clear that the present invention is implemented not by aforesaid way
Limitation, it is or not improved as long as the various unsubstantialities for employing inventive concept and technical scheme of the present invention progress are improved
The design of invention and technical scheme are directly applied into other occasions, within protection scope of the present invention.
Claims (3)
1. a kind of extracting method of high-purity berberine, including raw material stoving crush, add cellulase solution, extraction filtering,
Membrane filtration, separation, film concentration and dry series of steps, concrete operation step are as follows：
A, the coptis is put into baking oven dried at a temperature of 50-70 DEG C, then stir broken with machine；
B, the Golden Thread for obtaining step A add cellulase solution, and 2-3 hours are incubated at a temperature of 45-60 DEG C；
C, the comminution coptis for obtaining B add ethanol, and heating is boiled, and with filtered through gauze, filter residue is washed twice with ethanol；
D, under the high temperature conditions with ceramic film process, obtain ceramic membrane permeate and separation of ceramic film dope collected ceramic membrane and passed through
Liquid；
E, separating treatment carried out by seperation film, obtain seperation film permeate and seperation film dope, collect seperation film permeate；
F, by seperation film permeate by concentrate film carry out concentration, obtain concentration film concentrate and concentrate film permeate, receive
Collection concentration film concentrate；
G, concentration film dope is subjected to low-temperature vacuum drying, dries to powdered and take out, obtain finished product berberine.
2. a kind of extracting method of high-purity berberine according to claim 1, it is characterised in that the fiber in step B
The weight/mass percentage composition of plain enzyme solutions is 3%-5%.
3. a kind of extracting method of high-purity berberine according to claim 1, it is characterised in that second described in step C
Determining alcohol is 95%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710715731.8A CN107286152A (en) | 2017-08-20 | 2017-08-20 | A kind of extracting method of high-purity berberine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710715731.8A CN107286152A (en) | 2017-08-20 | 2017-08-20 | A kind of extracting method of high-purity berberine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107286152A true CN107286152A (en) | 2017-10-24 |
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ID=60106444
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710715731.8A Pending CN107286152A (en) | 2017-08-20 | 2017-08-20 | A kind of extracting method of high-purity berberine |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111329905A (en) * | 2018-12-18 | 2020-06-26 | 泰州医药城国科化物生物医药科技有限公司 | Total alkaloid component extracted from coptis chinensis and refining method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102807565A (en) * | 2012-07-30 | 2012-12-05 | 四川农业大学 | Improved method for extracting berberine |
| CN106518863A (en) * | 2016-09-23 | 2017-03-22 | 合肥信达膜科技有限公司 | Berberine film extraction technique |
-
2017
- 2017-08-20 CN CN201710715731.8A patent/CN107286152A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102807565A (en) * | 2012-07-30 | 2012-12-05 | 四川农业大学 | Improved method for extracting berberine |
| CN106518863A (en) * | 2016-09-23 | 2017-03-22 | 合肥信达膜科技有限公司 | Berberine film extraction technique |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111329905A (en) * | 2018-12-18 | 2020-06-26 | 泰州医药城国科化物生物医药科技有限公司 | Total alkaloid component extracted from coptis chinensis and refining method thereof |
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Application publication date: 20171024 |