CN103691498B - Liquid-moving machine and method of moving liquid by same - Google Patents
Liquid-moving machine and method of moving liquid by same Download PDFInfo
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- CN103691498B CN103691498B CN201410004737.0A CN201410004737A CN103691498B CN 103691498 B CN103691498 B CN 103691498B CN 201410004737 A CN201410004737 A CN 201410004737A CN 103691498 B CN103691498 B CN 103691498B
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Abstract
The invention provides a liquid-moving machine and a method of moving liquid by the same. The liquid-moving machine comprises a base, a vertical column arranged on one side of the base, a Z-axis motor arranged on the vertical column, a first lead screw arranged on an output shaft of the Z-axis motor, a slide block arranged on the first lead screw, a liquid-moving mechanism fixedly installed on the slide block and an electromagnetic positioning device which is arranged on the base and is electrically connected with the Z-axis motor, wherein the liquid-moving mechanism comprises a piston head connecting and fixing frame connected with the slide block, a piston motor assembled on the piston head connecting and fixing frame, a second lead screw connected with an output shaft of the piston motor, a piston fixing plate connected with the second lead screw, a plurality of pistons assembled on the piston fixing plate and piston cylinders assembled on the pistons. A plurality of insertion nozzles are arranged at the lower ends of the piston cylinders by corresponding to the pistons, a sealed convex ring and a positioning convex ring are arranged at the lower end of each insertion nozzle, a push rod is arranged on the piston fixing plate, and a sucker withdrawing push plate is arranged at the lower end of the push rod. The sucker withdrawing push plate is arranged below each piston cylinder and above the positioning convex ring.
Description
Technical field
The present invention relates to medical testing equipment field, particularly relate to a kind of pipettor and use it to carry out moving the method for liquid.
Background technology
Microwell plate, comprises the form such as 96 holes and 384 holes, is reaction vessel conventional in bioengineering and medical test.By there being a lot of apertures above it, a lot of test samples can being loaded and react under the same conditions, therefore be used widely in the investigation and application of biological and medical science.
Biological various reaction all be unable to do without various solution.In these apertures, add various reaction solution is very basic and important work.All the time, biomedical worker relies on piston type charger to carry out the interpolation of various solution to microwell plate.Its primary structure comprises:
1. sliding plunger: move up and down and cause air pressure change to carry out imbibition hydrojet;
2. adjusting screw(rod): determine that the height determination liquid absorption of piston upwards is how many;
3. spring: by the height of piston automatic rebounding to regulating nut place;
4. push rod: operator overcomes spring force with finger and promotes piston and moves downward with hydrojet;
5. interrupt: entangle plastic tips and form airtight environment so that liquid enters suction nozzle;
Piston type charger has the form of single head or 8 or 12.Adopt operator's finger to overcome the liquid-transfering device that spring force controls the form of hydrojet, be called traditional pipettor.
More novel liquid-transfering device does not then need operator to point to overcome spring force to control hydrojet, and have employed Electric Machine Control piston movement position to realize imbibition hydrojet.These liquid-transfering devices adjust the 2nd of above-mentioned traditional pipettor the, 3,4 dot structure, and its structure comprises:
1. sliding plunger: move up and down and cause air pressure change to carry out imbibition hydrojet;
2. motor and screw rod: determine that the movement position of piston is to realize quantitative liquid suction/hydrojet;
3. micro computer: stored routine also controls motor movement;
4. button and display screen: operator according to program interface in order to input service information;
5. interrupt: entangle plastic tips and form airtight environment so that liquid enters suction nozzle.
In prior art, the installation and removal of suction nozzle is all adopt artificial installation, on namely suction nozzle being fixed on by operator interrupting with hand.There are the following problems for manual installation suction nozzle:
1. manually insert suction nozzle and there is risk: manually insert suction nozzle and may cause and interrupt and seal between suction nozzle bad situation and cause and move liquid failure.Because there is certain difference in the direction of the size that staff is exerted oneself at every turn.Particularly, more easily there is this situation in the product of 8 or 12.
2. insert the fatigue of suction nozzle/desorption head: manually inserting suction nozzle needs firmly to insert, and manual desorption head makes the finger of operator tired downwards.Particularly the product of 8 or 12, needs very large strength just can be seated/decorporate.
Summary of the invention
The object of the invention is to the defect overcoming prior art, the pipettor of a kind of energy Auto-mounting and dismounting suction nozzle is provided.
Another object of the present invention is to provide a kind of method of moving liquid, the method is simple to operate, easy to control and fault rate is low.
For achieving the above object, the invention provides a kind of pipettor, comprise base, be located at the column of base side, the Z axis motor be located on column, the first screw mandrel being located at Z axis motor output shaft, the slide block be located on the first screw mandrel, be fixedly installed in the pipetting device on slide block and be located on base and the electromagnetic locator be electrically connected with Z axis motor, described pipetting device comprises the piston head Connection Bracket of connection sliding block, be installed in the piston motor on piston head Connection Bracket, the second screw mandrel be connected with the output shaft of piston motor, the piston fixed head be connected with the second screw mandrel, be installed in the several pistons on piston fixed head, be set in the piston/cylinder on piston, the corresponding piston in lower end of piston/cylinder is provided with several interrupting, described upper end of interrupting is for packed-piston cylinder body, described lower end of interrupting is for being socketed suction nozzle, described lower end of interrupting is respectively equipped with the sealing bulge loop being positioned at below and the directed bulge loop being positioned at top, described piston fixed head is provided with push rod, the lower end of push rod is provided with the push pedal of desorption head, this desorption head push pedal is arranged at below piston/cylinder, and be arranged at the top of the directed bulge loop of interrupting, described electromagnetic locator is provided with plate rail.
The upper end of described piston/cylinder is provided with sealing ring, described piston, sealing ring, piston/cylinder and form seal cavity between interrupting, described piston moves up and down and makes to produce in seal cavity pressure change in seal cavity, thus can promote liquid and enter or discharge and interrupt in the suction nozzle of below socket.
Described interrupting adopts flexible material to make.
Also comprise the touch screen control panel and operating grip that are electrically connected described Z axis motor, described piston motor and described electromagnetic locator.
Described piston is 96, described in interrupt be 96.
Described column is provided with the polished rod parallel with the first screw mandrel, and on described slide block, corresponding polished rod is provided with through hole, and described polished rod is located in this through hole.
Described electromagnetic locator comprises the guide rail be installed on base, slide be located at plate rail on guide rail, be located at guide rail relative to both sides locating piece, be located in plate rail and relative positioning block arrange electromagnet, be located on locating piece and opposed electromagnets arrange iron block, the crashproof rubber cushion be located in plate rail, be located at the microswitch in plate rail and be located on described locating piece and the set screw that arranges of corresponding described microswitch, described electromagnet is electrically connected described Z axis motor; Described electromagnetic locator also comprises to be located in plate rail and the first installation base be positioned at above guide rail and be located at plate rail and be positioned at the second installation base below guide rail, described electromagnet is located on this first installation base, be provided with spring between this electromagnet and the first installation base, described crashproof rubber cushion and described microswitch are all fixedly installed on this second installation base.
The distance D3 of the described set screw and microswitch that are positioned at the same side is less than the distance D1 of described electromagnet and described iron block, and the distance D1 of described electromagnet and described iron block is less than the distance D2 of described crashproof rubber cushion and described locating piece.
The present invention also provides a kind of method using above-mentioned pipettor to carry out moving liquid, comprises the following steps:
Step 1, pipettor is provided, the suction nozzle box of suction nozzle, source solution disk, target disc are housed, respectively suction nozzle box, source solution disk, target disc are placed on the plate rail of pipettor successively; Flexible material is adopted below described suction nozzle box;
Step 2, set respectively move liquid height H 1, suction range H2, tapping height H 3 according to source solution disk, target disc position;
Step 3, manual mobile plate rail, the suction nozzle box on plate rail is made to be positioned at immediately below interrupting of pipettor, control Z axis electric machine rotation, band movable slider moves downward, make the pipetting device entirety be fixedly installed on slide block move downward near suction nozzle box, to contact suction nozzle of interrupting, slow down and continue downwards, make to interrupt in malleation insertion suction nozzle, cannot move downward until interrupt; Control Z axis motor antiport, upwards mention pipetting device, complete the installation of suction nozzle thus;
Step 4, manual mobile plate rail, the source solution disk of making is positioned at immediately below suction nozzle, control the running of Z axis motor forward, suction nozzle position is made to arrive the suction range H2 of setting, then control piston motor positive movement sucks liquid in suction nozzle, then automatically controlling the counter motion of Z axis motor by software to mention to moving liquid height H 1, completing the sucking action of source solution;
Step 5, again manual mobile plate rail, target disc is made to be positioned at immediately below suction nozzle, control the running of Z axis motor forward, suction nozzle position is made to arrive the tapping height H 3 of setting, then the counter motion of control piston motor makes the liquid in suction nozzle be expelled in target disc, then automatically controlling the counter motion of Z axis motor by software to mention to moving liquid height H 1, completing and moving liquid.
Also comprise step 6, control piston motor continues counter motion, drive piston to move downward, discharge air; Manual mobile plate rail, makes suction nozzle box be positioned at immediately below suction nozzle, and control piston fixed head moves downward and promotes push rod and drive the push pedal of desorption head to produce a downward thrust to suction nozzle downwards, makes suction nozzle depart to interrupt and fall in suction nozzle box, completes the dismounting of suction nozzle thus.
Beneficial effect of the present invention: pipetting device, by arranging a Z axis motor, is connected by the first screw mandrel with Z axis motor by pipettor of the present invention, pipetting device can under the drive of Z axis motor, realize Auto-mounting suction nozzle, succinctly easy to operate, install firmly, workload is little; Pipettor of the present invention, by arranging the push pedal of desorption head, can automatically realize desorption head, greatly reduce the workload of experimenter; Adopt 96 pistons to carry out moving liquid can efficiently and reliably work, pay very large muscle power without the need to operator, greatly reduce the possibility of maloperation; And adopt electromagnetic locator as manual auxiliary equipment, coordinate the automatic control of motor, make to move liquid process accurate contraposition, simple to operate, operating efficiency is high.The method adopting pipettor of the present invention to carry out moving liquid is manually assisted and is automatically controlled to combine with motor, and simple to operate, precision is high, and operating efficiency is high.
Accompanying drawing explanation
Fig. 1 is the structural representation of pipettor of the present invention;
Fig. 2 is the partial structurtes schematic diagram of interrupting of pipettor of the present invention;
Fig. 3 is the partial sectional view of interrupting of pipettor of the present invention;
Fig. 4 is the structural representation that the electromagnetic locator of pipettor of the present invention is in microswitch normally open;
Fig. 5 is the structural representation that the electromagnetic locator of pipettor of the present invention is in microswitch closure state.
Detailed description of the invention
Mechanism of the present invention and operation principle is described in detail below in conjunction with accompanying drawing.In order to better the present embodiment is described, some parts of accompanying drawing have omission, zoom in or out, and do not represent the size of actual product; To those skilled in the art, in accompanying drawing, some known features and explanation thereof may be omitted is understandable.The corresponding same or analogous parts of same or analogous label.
Refer to shown in Fig. 1-5, the invention provides a kind of pipettor, comprise base 3, be located at the column 2 of base 3 side, the Z axis motor 21 be located on column 2, the first screw mandrel 22 being located at Z axis motor 21 output shaft, the slide block 24 be located on the first screw mandrel 22, be fixedly installed in the pipetting device 1 on slide block 24 and be located on base 3 and the electromagnetic locator 30 be electrically connected with Z axis motor 21.
Described pipetting device 1 comprises the piston head Connection Bracket 10 of connection sliding block 24, be installed in the piston motor 11 on piston head Connection Bracket 10, the second screw mandrel 111 be connected with the output shaft of piston motor 11, the piston fixed head 12 be connected with the second screw mandrel 111, be installed in the several pistons 14 on piston fixed head 12, be set in the piston/cylinder 16 on piston 14, the upper end of piston/cylinder 16 is provided with sealing ring 13, the corresponding piston in the lower end of piston/cylinder 16 is provided with severally interrupts 15, described 15 upper ends of interrupting are for packed-piston cylinder body 16, described 15 lower ends of interrupting are for being socketed suction nozzle 17.Described piston 14, sealing ring 13, piston/cylinder 16 and interrupt between 15 and form seal cavity 141, described piston 14 moves up and down and makes to produce pressure change in seal cavity 141 in seal cavity 141, thus can promote liquid and enter or discharge and interrupt in the suction nozzle 17 of socket below 15.When the second screw mandrel 111 moves upward, in seal cavity 141, pressure reduces, and liquid enters in suction nozzle 17 from the aperture of suction nozzle 17; When the second screw mandrel 111 moves downward, pressure increase in seal cavity 141, liquid is discharged from the aperture of suction nozzle 17.
When described Z axis motor 21 rotates, the first screw mandrel 22 can be driven to rotate, and slide block 24 moves up and down under the huge torsional forces effect of Z axis motor 21 and the first screw mandrel 22, thus drives pipetting device 1 to move up and down, to interrupt 15 insertion suction nozzles 17 compressing, to realize the function of Auto-mounting suction nozzle 17.
For guaranteeing to plug suction nozzle 17 and air tight, described in 15 lower ends of interrupting be respectively equipped with the sealing bulge loop 151 being positioned at below and the directed bulge loop 152 being positioned at top.Described interrupt 15 and sealing bulge loop 151 provided thereon and directed bulge loop 152 are formed in one and make, easy to process.Described 15 employing flexible materials of interrupting are made, and have certain amount of elastic deformation, are beneficial to install suction nozzle 17, and sealing effectiveness is better.Described sealing bulge loop 151 and directed bulge loop 152 are all provided at the arc bulge loop of interrupting outside 15.The contact area of itself and suction nozzle 17 is less, is easy to deeply insert in suction nozzle 17 be beneficial to installation, and sealing effectiveness is better.Described arranging of sealing bulge loop 151 height has larger impact to sealing effectiveness, if the excessive height of sealing bulge loop 151, bulge loop structure is not wear-resisting; If the height of sealing bulge loop 151 is too small, the accuracy of processing is difficult to ensure.Preferably, described sealing bulge loop 151 relatively interrupt 15 height be 0.2mm ~ 0.212mm, be not only beneficial to processing but also good sealing effectiveness can be ensured.
Described directed bulge loop 152 mainly 15 provides guide effect namely allow suction nozzle 17 direction accurate positioning to interrupting, and possesses certain sealing effectiveness simultaneously.Longitudinal contact length that described directed bulge loop 152 increases suction nozzle 17 and interrupts between 15, the angle that suction nozzle 17 is swung is restricted, thus gives suction nozzle 17 orientation to guarantee suction nozzle 17 straight down.Described sealing bulge loop 152 relatively interrupt 15 height be 0.18mm ~ 0.192mm, there is preferably directed and sealing function.For coordinating the installation of suction nozzle 17, adopt flexible material below the suction nozzle box depositing suction nozzle 17, the adjustment making suction nozzle 17 in installation suction nozzle 17 process can make certain angle because of Impact direction interrupts 15 better to fit, and completes sealing.When suction nozzle 17 is installed, the sealing bulge loop 151 of interrupting on 15 utilizes its cambered design to insert in suction nozzle 17, then the guide effect of directed bulge loop 152 is relied on, and coordinate the reverse extrusion of suction nozzle box, to interrupt and 15 to insert securely in suction nozzle 17, because sealing bulge loop 151 and the frictional resistance between directed bulge loop 152 and suction nozzle 17 inwall make mounted suction nozzle 17 15 fall down from interrupting easily.
Described piston fixed head 12 is provided with push rod 182, the lower end of push rod 182 is provided with desorption head push pedal 181, this desorption head push pedal 181 is arranged at below piston/cylinder 16, and be arranged at interrupt 15 the top of directed bulge loop 152, this desorption head push pedal 181 can move up and down between piston/cylinder 16 lower end and the upper end of directed bulge loop 152.During the running of piston motor 11 forward, the second screw mandrel 111 is driven to rotate, its torsional forces produced drives piston fixed head 12 to move down, described piston 14, push rod 182, and suction nozzle push pedal 181 moves down under the effect of piston fixed head 12, be defined as piston 14 when suction nozzle push pedal 181 moves to the position that can contact suction nozzle 17 upper end and be positioned at zero graduation line position, control piston motor 11 continues forward running, move downward making piston fixed head 12 and promote push rod 182 and drive downwards desorption head push pedal 181 pairs of suction nozzles 17 to produce a downward thrust, suction nozzle 17 is departed from and interrupts 15.
Further, described column 2 is provided with the polished rod 23 parallel with the first screw mandrel 22, and on described slide block 24, corresponding polished rod 23 is provided with through hole 241, and described polished rod 23 is located in this through hole 241, thus ensures that pipetting device 1 moving up and down on column 2 is more stable.
Described pipettor also comprises the touch screen control panel (not shown) and operating grip (not shown) that are electrically connected described Z axis motor 21, described piston motor 11 and described electromagnetic locator 30, in order to acknowledge(ment) signal and input of control commands, thus control Z axis motor 21, piston motor 11 and electromagnetic locator 30 respectively.
Described electromagnetic locator 30 comprise the guide rail 31 be installed on base 3, the plate rail 32 be located on guide rail 31, be located at guide rail 31 relatively both sides locating piece 33, be located in plate rail 32 and be positioned at the first installation base 321 above guide rail 31, be located at plate rail 32 and relative positioning block 33 arrange electromagnet 322, be located on locating piece 33 and the iron block 331 of opposed electromagnets 322 setting.Described electromagnet 322 is located on this first installation base 321, is provided with spring 323, has certain cushioning effect between described electromagnet 322 and the first installation base 321.Described electromagnet 322 is electrically connected described Z axis motor 21.Described plate rail 32 is for placing the liquid-transfering devices such as suction nozzle box, source solution disk and/or target disc, needs according to moving liquid process are selected, described plate rail 32 can move horizontally on guide rail 31, to guarantee that the liquid-transfering device be positioned on plate rail 32 moves to suitable position.Described source solution disk and target disc all can be microwell plate, comprise the form such as 96 holes and 384 holes.Accordingly, preferred described piston 14 is 96, described in interrupt 15 be also 96, be convenient to disposable complete 96 holes move liquid, and the liquid that moves in 384 holes only needs to carry out four times and operates, very convenient.Described electromagnetic locator 30 also comprises to be located in plate rail 32 and to be positioned at the second installation base 324 below guide rail 31, to be located at the crashproof rubber cushion 325 of plate rail 32, to be located at the microswitch 326 of plate rail 32 and to be located on described locating piece 33 and the set screw 332 that arranges of corresponding described microswitch 326.Described crashproof rubber cushion 325 and described microswitch 326 are all fixedly installed on described second installation base 324.When microswitch 326 contacts with set screw 332, microswitch 326 is in closure state; When microswitch 326 does not contact with set screw 332, microswitch 326 is in normally open.
During installation, the distance D3 of the described set screw 332 and microswitch 326 that are positioned at the same side is less than the distance D1 of described electromagnet 322 and described iron block 331, and described electromagnet 322 and the distance D1 of described iron block 331 are less than the distance D2 of described crashproof rubber cushion 325 and described locating piece 33.Preferably, D3 about 0.2mm, D1 less of D1 about 0.5mm less of D2.When manual mobile plate rail 32 arrives the locating piece 33 of guide rail 31 side, set screw 332 contacts at first with microswitch 326, because microswitch 326 has certain stroke, thus can not be damaged, then electromagnet 322 contacts with iron block 33, and now electromagnet 322 can certain amplitude retreat due to the cushioning effect of spring 323, last crashproof rubber cushion 325 contacts with locating piece 33, because the elasticity of crashproof rubber cushion 325 can prevent overshoot, and stop motion, now, plate rail 32 arrives precalculated position, be positioned over liquid-transfering device on plate rail 32 can with 15 accurate contrapositions of interrupting, microswitch 326 contacts with set screw 332, microswitch 326 is in closure state, microswitch 326 sends the signal moved into place, permission Z axis motor 21 moves by software, open Z axis motor 21 to operate, the first screw mandrel 22 is driven to rotate, move under making slide block 24 in the vertical direction, thus drive pipetting device 1 to move up and down.In Z axis motor 21 operation process, electromagnet 322 is energized and produces powerful magnetic force, guarantee plate rail 32 be fixed abut in guide rail 31 side locating piece 33 on (referring to Fig. 5), avoid now External Force Acting skew plate rail 32 and cause moving liquid to bit-errors.When needs move plate rail 32 time, the first power-off of electromagnet 322, then operator can move with hand propelled plate rail 32.When plate rail 32 leaves the locating piece 33 at guide rail 31 two ends, microswitch 326 has departed from the compressing of set screw 332 and has again been in open mode.Now illustrate that plate rail 32 is not on correct position, microswitch 326 has been in open mode and has can be used as the signal that a movement do not put in place, and software does not allow Z axis motor 21 to carry out operating by not being performed and points out operator to need mobile correct position.
Electromagnetic locator 30 of the present invention, is positioned by crashproof rubber cushion 325, and electromagnet 322 carries out the protection of position, the signal that microswitch 326 judges as software.The locking that whole electromagnetic locator 30 does not need operator manually to intervene plate rail 32 locks with solution, and does not affect operating handle, does not increase the not extra operation of operator and notice.The localization method of liquid-transfering device is very simple and convenient, and can guarantee operator at every turn movement can move to correct position, and without the need to operator spend extra energy go process positioning precision.
In sum, pipetting device, by arranging a Z axis motor, is connected by the first screw mandrel with Z axis motor by pipettor of the present invention, and pipetting device under the drive of Z axis motor, can realize Auto-mounting suction nozzle, succinctly easy to operate, and install firmly, workload is little; Pipettor of the present invention, by arranging the push pedal of desorption head, can automatically realize desorption head, greatly reduce the workload of experimenter; Adopt 96 pistons to carry out moving liquid can efficiently and reliably work, pay very large muscle power without the need to operator, greatly reduce the possibility of maloperation; And adopt electromagnetic locator as manual auxiliary equipment, coordinate the automatic control of motor, make to move liquid process accurate contraposition, simple to operate, operating efficiency is high.
In addition, the present invention also provides a kind of method using above-mentioned pipettor to carry out moving liquid, comprises the following steps:
Step 1, pipettor is provided, the suction nozzle box of suction nozzle, source solution disk, target disc are housed, respectively suction nozzle box, source solution disk, target disc are placed on the plate rail of pipettor successively.Flexible material is adopted below described suction nozzle box.
Step 2, set respectively move liquid height H 1, suction range H2, tapping height H 3 according to source solution disk, target disc position.
Step 3, suction nozzle to be installed on pipettor.
Non-follow control moves plate rail, the suction nozzle box on plate rail is made to be positioned at immediately below interrupting of pipettor, control Z axis electric machine rotation, band movable slider moves downward, make the pipetting device entirety be fixedly installed on slide block move downward near suction nozzle box, to contact suction nozzle of interrupting, slow down and continue downwards, make to interrupt in malleation insertion suction nozzle, cannot move downward until interrupt.Control Z axis motor antiport, upwards mention pipetting device, complete the installation of suction nozzle thus.This pipettor disposablely can complete the installation of 96 suction nozzles, and adopts mechanical control method, pressure uniform downward during installation, and easy for installation being easy to of whole process controls, and suction nozzle good sealing effect.
Step 4, absorption source solution.
When Z axis motor shuts down, electromagnet is in off-position, can direct manual mobile plate rail, the source solution disk of making is positioned at immediately below suction nozzle, control the running of Z axis motor forward, make suction nozzle position arrive the suction range H2 of setting, then control piston motor positive movement sucks liquid in suction nozzle, then automatically controlling the counter motion of Z axis motor by software to mention to moving liquid height H 1, namely completing the sucking action of source solution.
Step 5, source solution to be discharged in target disc.
Manual mobile plate rail again, target disc is made to be positioned at immediately below suction nozzle, control the running of Z axis motor forward, suction nozzle position is made to arrive the tapping height H 3 of setting, then the counter motion of control piston motor makes the liquid in suction nozzle be expelled in target disc, then automatically controlling the counter motion of Z axis motor by software to mention to moving liquid height H 1, completing and moving liquid.
Step 6, dismounting suction nozzle.
Control piston motor continues counter motion, drives piston to move downwardly to extreme position, discharges air.Manual mobile plate rail, makes suction nozzle box be positioned at immediately below suction nozzle.Control piston fixed head moves downward and promotes push rod and drive the push pedal of desorption head to produce a downward thrust to suction nozzle downwards, makes suction nozzle depart to interrupt and fall in suction nozzle box, completes the dismounting of suction nozzle thus.
The method adopting pipettor of the present invention to carry out moving liquid is manually assisted and is automatically controlled to combine with motor, and simple to operate, precision is high, and operating efficiency is high.
Obviously, the above embodiment of the present invention is only for example of the present invention is clearly described, and is not the restriction to embodiments of the present invention.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without the need to also giving all embodiments.All any amendments done within the spirit and principles in the present invention, equivalent to replace and improvement etc., within the protection domain that all should be included in the claims in the present invention.
Claims (9)
1. a pipettor, it is characterized in that, comprise base, be located at the column of base side, the Z axis motor be located on column, the first screw mandrel being located at Z axis motor output shaft, the slide block be located on the first screw mandrel, be fixedly installed in the pipetting device on slide block and be located on base and the electromagnetic locator be electrically connected with Z axis motor, described pipetting device comprises the piston head Connection Bracket of connection sliding block, be installed in the piston motor on piston head Connection Bracket, the second screw mandrel be connected with the output shaft of piston motor, the piston fixed head be connected with the second screw mandrel, be installed in the several pistons on piston fixed head, be set in the piston/cylinder on piston, the corresponding piston in lower end of piston/cylinder is provided with several interrupting, described upper end of interrupting is for packed-piston cylinder body, described lower end of interrupting is for being socketed suction nozzle, described lower end of interrupting is respectively equipped with the sealing bulge loop being positioned at below and the directed bulge loop being positioned at top, described piston fixed head is provided with push rod, the lower end of push rod is provided with the push pedal of desorption head, this desorption head push pedal is arranged at below piston/cylinder, and be arranged at the top of the directed bulge loop of interrupting, described electromagnetic locator is provided with plate rail, described electromagnetic locator comprises the guide rail be installed on base, slide be located at plate rail on guide rail, be located at guide rail relative to both sides locating piece, be located in plate rail and relative positioning block arrange electromagnet, be located on locating piece and opposed electromagnets arrange iron block, the crashproof rubber cushion be located in plate rail, be located at the microswitch in plate rail and be located on described locating piece and the set screw that arranges of corresponding described microswitch, described electromagnet is electrically connected described Z axis motor, described electromagnetic locator also comprises to be located in plate rail and the first installation base be positioned at above guide rail and be located at plate rail and be positioned at the second installation base below guide rail, described electromagnet is located on this first installation base, be provided with spring between this electromagnet and the first installation base, described crashproof rubber cushion and described microswitch are all fixedly installed on this second installation base.
2. pipettor as claimed in claim 1, it is characterized in that, the upper end of described piston/cylinder is provided with sealing ring, described piston, sealing ring, piston/cylinder and form seal cavity between interrupting, described piston moves up and down and makes to produce in seal cavity pressure change in seal cavity, thus can promote liquid and enter or discharge and interrupt in the suction nozzle of below socket.
3. pipettor as claimed in claim 1, is characterized in that, described in interrupt and adopt flexible material to make.
4. pipettor as claimed in claim 1, is characterized in that, also comprises the touch screen control panel and operating grip that are electrically connected described Z axis motor, described piston motor and described electromagnetic locator.
5. pipettor as claimed in claim 1, it is characterized in that, described piston is 96, described in interrupt be 96.
6. pipettor as claimed in claim 1, it is characterized in that, described column is provided with the polished rod parallel with the first screw mandrel, and on described slide block, corresponding polished rod is provided with through hole, and described polished rod is located in this through hole.
7. pipettor as claimed in claim 1, it is characterized in that, the distance D3 of the described set screw and microswitch that are positioned at the same side is less than the distance D1 of described electromagnet and described iron block, and the distance D1 of described electromagnet and described iron block is less than the distance D2 of described crashproof rubber cushion and described locating piece.
8. use as the pipettor in claim 1 ~ 7 as described in any one carries out a method of moving liquid, it is characterized in that, comprise the following steps:
Step 1, pipettor is provided, the suction nozzle box of suction nozzle, source solution disk, target disc are housed, respectively suction nozzle box, source solution disk, target disc are placed on the plate rail of pipettor successively; Flexible material is adopted below described suction nozzle box;
Step 2, set respectively move liquid height H 1, suction range H2, tapping height H 3 according to source solution disk, target disc position;
Step 3, manual mobile plate rail, the suction nozzle box on plate rail is made to be positioned at immediately below interrupting of pipettor, control Z axis electric machine rotation, band movable slider moves downward, make the pipetting device entirety be fixedly installed on slide block move downward near suction nozzle box, to contact suction nozzle of interrupting, slow down and continue downwards, make to interrupt in malleation insertion suction nozzle, cannot move downward until interrupt; Control Z axis motor antiport, upwards mention pipetting device, complete the installation of suction nozzle thus;
Step 4, manual mobile plate rail, the source solution disk of making is positioned at immediately below suction nozzle, control the running of Z axis motor forward, suction nozzle position is made to arrive the suction range H2 of setting, then control piston motor positive movement sucks liquid in suction nozzle, then automatically controlling the counter motion of Z axis motor by software to mention to moving liquid height H 1, completing the sucking action of source solution;
Step 5, again manual mobile plate rail, target disc is made to be positioned at immediately below suction nozzle, control the running of Z axis motor forward, suction nozzle position is made to arrive the tapping height H 3 of setting, then the counter motion of control piston motor makes the liquid in suction nozzle be expelled in target disc, then automatically controlling the counter motion of Z axis motor by software to mention to moving liquid height H 1, completing and moving liquid.
9. move the method for liquid as claimed in claim 8, it is characterized in that, also comprise step 6, control piston motor continues counter motion, drive piston to move downward, discharge air; Manual mobile plate rail, makes suction nozzle box be positioned at immediately below suction nozzle, and control piston fixed head moves downward and promotes push rod and drive the push pedal of desorption head to produce a downward thrust to suction nozzle downwards, makes suction nozzle depart to interrupt and fall in suction nozzle box, completes the dismounting of suction nozzle thus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410004737.0A CN103691498B (en) | 2014-01-06 | 2014-01-06 | Liquid-moving machine and method of moving liquid by same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410004737.0A CN103691498B (en) | 2014-01-06 | 2014-01-06 | Liquid-moving machine and method of moving liquid by same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103691498A CN103691498A (en) | 2014-04-02 |
| CN103691498B true CN103691498B (en) | 2015-05-27 |
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| CN104001563B (en) * | 2014-06-09 | 2015-07-08 | 上海优爱宝机器人技术有限公司 | Pipettor |
| EP3159397B1 (en) * | 2014-06-17 | 2018-05-02 | Yamaha Hatsudoki Kabushiki Kaisha | Head device for mounting dispensing tip thereon, and movement device using same |
| CN104353510B (en) * | 2014-10-27 | 2016-03-02 | 佛山市德淙科学仪器有限公司 | A kind ofly can fold the liquid-transfering device of box-packed suction nozzle by Auto-mounting |
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| CN111781390B (en) * | 2020-07-27 | 2023-09-05 | 苏州奥美泰克生物技术有限公司 | Four-channel double-circulation synchronous sample transferring system and method |
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| CN113976199A (en) * | 2021-11-02 | 2022-01-28 | 美东汇成生命科技(昆山)有限公司 | Liquid-transfering suction head that practicality is high |
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| CN116908479B (en) * | 2023-08-07 | 2024-04-19 | 山东省动物疫病预防与控制中心(山东省人畜共患病流调监测中心) | Full-automatic hemagglutination and hemagglutination inhibition test pipetting device |
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