CN103664341B - Fertilizer and method for increasing anthocyanin content in potatoes - Google Patents
Fertilizer and method for increasing anthocyanin content in potatoes Download PDFInfo
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- CN103664341B CN103664341B CN201310569110.5A CN201310569110A CN103664341B CN 103664341 B CN103664341 B CN 103664341B CN 201310569110 A CN201310569110 A CN 201310569110A CN 103664341 B CN103664341 B CN 103664341B
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Abstract
The invention discloses a fertilizer and method for increasing the anthocyanin content in purple potatoes. The fertilizer contains 1-5% of white granulated sugar, 100-300ppm of ammonium citrate, 50-100ppm of methyl jasmonate and 20-40ppm of phenylalanine. According to the invention, the anthocyanin content in purple potatoes can be obviously increased, thereby solving the problem that the anthocyanin content in purple potatoes is low. Anthocyanin has very high economic value; and the invention has wide application prospects.
Description
Technical field
The present invention relates to a kind of fertilizer and the method that improve anthocyanidin content in purple-colored potato.
Background technology
" anthocyanidin " is natural potent free-radical scavengers, and have preventive and therapeutic action to 100 various diseases, anthocyanidin is described as: the seventh-largest essential nutrients after water, protein, fat, carbohydrate, VITAMIN, mineral substance." anthocyanidin " be current scientific circles find disease preventing and treating, safeguard human health the most directly, the most effective, safest free-radical scavengers, it is eliminated, and ability of free radical is ascorbic 20 times, 50 times of vitamin-E.Simultaneously anthocyanidin uniquely through the material of hemato encephalic barrier scavenging free radicals protection brain cell, can reduce microbiotic to some harm of human body, and purple-colored potato, can as one of main raw material extracting " anthocyanidin " because biological yield is high.But anthocyanidin content (only containing 4-5 milligram in every 100 grams) on the low side in purple-colored potato, chemical synthesis process is difficult to realize suitability for industrialized production.Therefore can find and a kind ofly improve the method for anthocyanidin content in purple-colored potato and seem particularly important.Not yet have so far and adopt technological method to improve the report of anthocyanidin content to purple-colored potato.
Summary of the invention
The object of the invention is to for the too low problem of anthocyanidin content in above-mentioned purple-colored potato, a kind of fertilizer and the method that can improve anthocyanidin content in purple-colored potato are provided, solve the problem that in purple-colored potato, anthocyanidin content is too low.
Technical scheme provided by the invention is: a kind of fertilizer improving anthocyanidin content in purple-colored potato, white sugar in this fertilizer: 1-5%; Ammonium citrate: 100-300ppm; Methyl jasmonate: 50-100ppm; Phenylalanine: 20-40ppm, wherein the amount of white sugar is the mass ratio that white sugar accounts for described fertilizer.
Preferably, in described fertilizer, methyl jasmonate: 100ppm.
Preferably, fertilizer according to claim 2, is characterized in that: in described fertilizer, white sugar: 3%; Ammonium citrate: 100ppm; Methyl jasmonate: 100ppm; Phenylalanine: 30ppm.
Preferably, fertilizer according to claim 2, is characterized in that: in described fertilizer, white sugar: 1%; Ammonium citrate: 300ppm; Methyl jasmonate: 100ppm; Phenylalanine: 40ppm.
Particularly preferably, in described fertilizer, white sugar: 5%; Ammonium citrate: 200ppm; Methyl jasmonate: 100ppm; Phenylalanine: 20ppm.
The compound method of above-mentioned fertilizer is as follows:
(1) Sucrose Sources: commercially available white sugar; (2) citric acid source: commercial anhydrous ammonium citrate (Han Liang≤99.9%)
(3) methyl jasmonate source: commercially available methyl jasmonate (purity 98%); (4) phenylalanine source: commercially available L-Phe (Han Liang≤98.5%)
By water-soluble respectively for above-mentioned each component, after being mixed with suitable concentration, mixing is shared equally even.
The present invention also provides described fertilizer to improve the method for anthocyanidin content in purple-colored potato, the method is that described fertilizer is respectively sprayed 1 time in potato tuber Formation period and harvesting time, impose on potato upper blade, each consumption is every strain 10 milliliters, evenly sprays in potato leaf surface.
The present invention has following beneficial effect:
The present invention, by a large amount of experimental studies, finds that under the condition of suitable cultivation potato, apply appropriate fertilizer of the present invention by the inventive method can improve anthocyanidin content in purple-colored potato, thus solves the low difficult problem of anthocyanidin content in purple-colored potato.Anthocyanidin has high economic worth, is widely used in cancer prevention, the chemical field such as medical field and cosmetic industry such as eyesight improves, anti-oxidant and cardiovascular problems improvement.Apply fertilizer formula of the present invention, can greatly improve anthocyanidin content in existing potato, for anthocyanidin industrial processes industry provides more high-quality, inexpensive anthocyanidin raw material, simultaneously for human consumer provides more reliable anthocyanidin food raw material.Of the present inventionly to have a extensive future.
Embodiment
Below by specifically
The detailed description of embodiment illustrates the present invention further, but is not limitation of the present invention, only does example explanation.
embodiment 1:
Preparation fertilizer, formula is as table 1.
(1) Sucrose Sources: commercially available white sugar;
(2) citric acid source: commercial anhydrous ammonium citrate (Han Liang≤99.9%);
(3) methyl jasmonate source: commercially available methyl jasmonate (purity 98%);
(4) phenylalanine source: commercially available L-Phe (Han Liang≤98.5%).
Formula (establishing blank) is mixed with according to orthogonal design scheme, each for above-mentioned fertilizer component is water-soluble respectively, mix after being mixed with suitable concentration.
Virus-free seed potato (kind name: purple ocean) is provided by Hunan Province's potato Engineering Technical Research Centre, and in sowing on December 25th, 2012, on April 27th, 2013 gathers in the crops.3 repetitions are established in each process, each repetition 120 strain.
Potato planting is according to orthogonal design scheme implementation, separately establish blank, tuber initiation stage and stem tuber spray formula fertilizer respectively according to orthogonal design embodiment harvesting time, impose on the upper blade of potato, each consumption is every strain 10 milliliters, evenly sprays in potato leaf surface.Weigh, for chemical analysis subsequently during every strain plant results.
After result shows to adopt fertilizer of the present invention and planting type, potato anthocyanidin content is up to 168.4 mg/100g(sucrose 5%, ammonium citrate 200ppm, methyl jasmonate 100ppm, phenylalanine 20ppm), next is 131.9 mg/100g(sucrose 3%, ammonium citrate 100ppm, methyl jasmonate 100ppm, phenylalanine-3,4-quinone 0ppm), be 130.3 mg/100g(sucrose 1% again, ammonium citrate 300ppm, methyl jasmonate 100ppm, phenylalanine 40ppm), the anthocyanidin content not using above-mentioned soil fertility quality is 4.3 mg/100g.Through improving 21.64 times with anthocyanidin content in potato after fertilizer of the present invention and fertilizing method fertilising, considerably improve anthocyanidin content in potato.
Table 1
Claims (6)
1. improve a fertilizer for anthocyanidin content in purple-colored potato, it is characterized in that: white sugar in this fertilizer: 1-5%; Ammonium citrate: 100-300ppm; Methyl jasmonate: 50-100ppm; Phenylalanine: 20-40ppm.
2. fertilizer according to claim 1, is characterized in that: in described fertilizer, methyl jasmonate: 100ppm.
3. fertilizer according to claim 2, is characterized in that: in described fertilizer, white sugar: 5%; Ammonium citrate: 200ppm; Methyl jasmonate: 100ppm; Phenylalanine: 20ppm.
4. fertilizer according to claim 2, is characterized in that: in described fertilizer, white sugar: 3%; Ammonium citrate: 100ppm; Methyl jasmonate: 100ppm; Phenylalanine: 30ppm.
5. fertilizer according to claim 2, is characterized in that: in described fertilizer, white sugar: 1%; Ammonium citrate: 300ppm; Methyl jasmonate: 100ppm; Phenylalanine: 40ppm.
6. the fertilizer according to any one of claim 1-5 improves the method for anthocyanidin content in purple-colored potato, it is characterized in that: the fertilizer described in claim 1-5 is respectively sprayed 1 time in potato tuber Formation period and harvesting time, impose on potato upper blade, each consumption is every strain 10 milliliters, evenly sprays in potato leaf surface.
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CN103664341B true CN103664341B (en) | 2015-07-15 |
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CN101283685B (en) * | 2008-06-10 | 2010-12-01 | 辽宁石油化工大学 | Preparation and producing method for promoting the Nanguo pear coloring |
MX2008013788A (en) * | 2008-10-16 | 2010-04-16 | Miguel Angel Martinez Tellez | Method for controlling the table grape colouring, from oligogalacturonides. |
JP5869773B2 (en) * | 2011-03-31 | 2016-02-24 | 株式会社フジキン | Anthocyanin production method |
CN103011980B (en) * | 2011-06-30 | 2014-04-30 | 湖南农业大学 | Fertilizer for increasing sulforaphane content in cabbage mustard and method |
CN102295490B (en) * | 2011-06-30 | 2013-07-31 | 湖南农业大学 | Fertilizer capable of improving content of sulforaphane in cultivated vegetable and method thereof |
CN102669644B (en) * | 2012-05-24 | 2013-07-24 | 浙江大学 | Method for promoting coloring of red Chinese pears by utilizing chemical agents |
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