CN103642712A - Composite flora for degrading papermaking waste water and preparation method thereof - Google Patents
Composite flora for degrading papermaking waste water and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a composite flora for degrading papermaking waste water and a preparation method thereof, wherein the preparation method comprises: respectively selecting Agrobacterium sp., Bacillus sp., Enterobacter cloacae., Pseudomonas putida. and Pseudomonas stutzeri. for inoculated culture; according to volume percentage, respectively selecting and 5-8% of Agrobacterium sp., 2-5% of Bacillus sp., 10-15% of Enterobacter cloacae, 26-41% of Pseudomonas putida, and 34-56% of Pseudomonas stutzeri., and mixing them and throwing them into waste water after activation. The composite flora for degrading papermaking waste water, by synergistic effects among the bacteria, can effectively raise efficiency of micro-biologically degrading pulping and papermaking waste water.
Description
Technical field
The present invention relates to paper waste process field, be specifically related to a kind of for the composite flora of paper waste and preparation method thereof of degrading.
Background technology
Biological treatment is current conventional method of wastewater treatment, and this method, by the metabolism of microorganism, decomposes the pollution substance in waste water, absorb, thereby reach, administers the object of polluting.Biological treatment is compared with additive method, and its cost is low, and efficiency is high, and easily operation, and the most important thing is does not have secondary pollution, therefore, in wastewater treatment, is widely used.Along with expanding economy, the composition of waste water is day by day complicated, while especially containing the organic pollutant of poisonous, difficult degradation in waste water, due to kind, the comparatively small amt of the microorganism this type organic to special degradation capability in environment, it is in a disadvantageous position in interspecific competition simultaneously, therefore, traditional biologic treating technique faces big challenge.If add microorganism or some matrix with specific function in traditional biological treatment system, strengthen its degradation capability to specific pollutants, thereby improve the treatment effect of whole sewage disposal system, we claim that this technology is biological reinforcing technology.
The microorganism adding in biological reinforcing technology can derive from original system for handling, passes through domestication, enrichment, screening, cultivation, thereby reaches the microorganism of some amount, can be also original non-existent inoculating microbe or genetically engineered bacteria.Wherein the stability of dominant bacteria in system is the key point that determines bioaugmentation disposal.Our early-stage Study shows the interaction between can microorganisms population through statistical method, and screening forms microorganism dominant population, and its bioaugmentation is better than the Screening of high efficient paracetamolum of single culture.
Because paper waste contains a large amount of recalcitrant substances, the biochemical treatment efficiency of paper-making effluent is lower, the mark of carrying along with the emission standard of paper-making effluent, COD, BOD and the toxic substance that can effectively remove in paper-making effluent become most important, by the structure of composite flora in biochemical system, can effectively improve the degraded of BOD and COD, the adding and working cost of chemical of reducing follow-up materialization system.
Summary of the invention
The object of the invention is to for the deficiencies in the prior art, provide the organic matter degradation efficiency in a kind of paper waste high, cost low for the composite flora of paper waste and preparation method thereof of degrading.
Object of the present invention is achieved through the following technical solutions:
A preparation method for the composite flora of the paper waste of degrading, comprises the steps:
The preparation of (1) five kind of bacterium logarithmic phase cell: difference picking edaphic bacillus (Agrobacterium sp.), rod bacterium (Bacillus sp.), enterobacter cloacae (Enterobacter.cloacae.), pseudomonas putida (Pseudomonas putida.), five kinds of bacterium of Pseudomonas stutzeri (Pseudomonas stutzeri.), 1～2 ring, it is transferred to respectively containing in 20～50mL nutritive medium, every kind of bacterium is cultivated 1～2 day under the condition of 27～35 ℃, again five kinds of bacterium after cultivating are seeded to containing in the container of 300～500mL proliferated culture medium with the volume ratio of 1:9～1:12 respectively by thalline and proliferated culture medium volume ratio, adopt different proliferated culture mediums to cultivate.Wherein, the nutritional medium of edaphic bacillus, rod bacterium and enterobacter cloacae is extractum carnis 1.5g～2.0/L, glucose 1.0～2.0g/L, and Tryptones 6.0～7.0g/L, yeast powder 3.0～4.0g/L, all the other are water; Pseudomonas stutzeri and pseudomonas putida nutritional medium are fresh potato juice 800～1000mL, glucose 16～20g, all the other are the water (preparation method of potato juice: remove 160～200 grams of skin fresh potatos, be cut into small pieces, adding deionized water 800～1000mL boils 30 minutes, elimination potato ball, complements to 800～1000mL with deionized water by filtrate).Then under the condition of 27～35 ℃, cultivate 1～2 day, after the centrifugal 10～15min of speed with 6000～7000rpm, obtain respectively the logarithmic phase cell of above-mentioned five kinds of thalline.Described nutritive medium main component is extractum carnis 1.5～2.0g/L, glucose 1.0～2.0g/L, and Tryptones 5.5～6.5g/L, yeast powder 3.0～4.0g/L, pH6.5～7.5, all the other are water;
The mixed bacterial of (2) five kinds of thalline composite: the logarithmic phase cell of described five kinds of thalline is taken out, with after phosphate buffered saline buffer washing 2～3 times, per-cent meter by volume, get respectively 5～8% edaphic bacilluss (Agrobacterium sp.), 2～5% rod bacteriums (Bacillus sp.), 10～15% enterobacter cloacaes (Enterobacter cloacae), 26～41% pseudomonas putidas (Pseudomonas putida) and 34～56% Pseudomonas stutzeris (Pseudomonas stutzeri.) mix; Must be for the composite flora of the paper waste of degrading.
Preferably, during described composite flora degraded paper waste, the mixed bacteria liquid of getting composite flora is added in nutritive medium and cultivates 6～12h with the volume ratio of 1:15～1:30, makes directly to put in paper waste after composite flora activation, and every 1 liter of paper waste adds mixed bacteria liquid 1～2mL; Air Exposure 2～4d, aeration rate is 2～4L/h.Described nutritive medium main component is extractum carnis 1.5～2.0g/L, glucose 1.0～2.0g/L, and Tryptones 5.5～6.5g/L, yeast powder 3.0～4.0g/L, pH6.5～7.5, all the other are water.
Per-cent meter by volume, the composition of described phosphate buffered saline buffer is sodium-chlor 8.0～9.0g/L, Repone K 0.2～0.3g/L, dipotassium hydrogen phosphate 1.1～1.2g/L and potassium primary phosphate 0.2～0.3g/L, all the other are water.
The washing times of described phosphate buffered saline buffer is 2～3 times.
For a composite flora for lignin degrading, by above-mentioned preparation method, made.
Tool of the present invention has the following advantages:
1) edaphic bacillus (Agrobacterium sp.) that utilizes provided by the invention, rod bacterium (Bacillus sp.), enterobacter cloacae (Enterobacter.Cloacae.), pseudomonas putida (Pseudomonas putida.), five kinds of bacterium of Pseudomonas stutzeri (Pseudomonas stutzeri.) form by a certain percentage advantage composite flora and add the biological reinforced processing of carrying out waste water: pseudomonas putida is often used in wastewater treatment, it has for example ability of toluene and phenol of degraded arene, and these are all the common pollutents in municipal wastewater; Rod bacterium is a kind of common soil bacteria and can makes aromatic hydrocarbon produce secondary degraded, and the aromatic series organic pollutants such as Pentachlorophenol, Poly Brominated Diphenyl Ethers are had to certain Degradation; And edaphic bacillus is to separate bacterial classification from soil, for pollutents such as the most organic pollutants in soil and heavy metals, all there is certain Degradation.The present invention finds that the degradation effect of composite flora is far above the single bacterial strain of any strain, illustrates and between different microorganisms, produces certain interaction.This,, because many biological activities are that individual plant Institute of Micro-biology can not complete or can only faintly carry out, must realize by two or more microorganisms in the interaction in same environment.Adopt single flora and genetic engineering bacterium to carry out in contaminant degradation process, usually because the generation of inhibition mesostate or intermediate product enter cut-off type meta-bolites approach (end product pathways), suppressed contaminant degradation enzymic activity, made that contaminant degradation efficiency is not high or degraded is not thorough.Utilize interaction useful between microorganism, by microorganism mixed culture and domestication, bacterium is flowed to and carries out some improvement the mesostate of pollutent, inhibition intermediate product do not generated or transform as early as possible, thereby improving contaminant degradation efficiency.Therefore, utilize microbial interaction, the mixt bacteria microorganism culturing method for domesticating adopting in the practical application of microorganism has more significance.
2) in the present invention, we find that edaphic bacillus and the rod bacterium of from be subject to electronic waste contaminated soil, separating can produce nicotinic acid hydroxylated enzyme and ring opening dioxygenase etc., Pseudomonas stutzeri can produce wooden equal hexichol for Yi Xi ?α ， β ?the peroxidase such as dioxygenase.These endonuclease capables have very strong Degradation to the multiple aromatics of paper waste.Especially the xylogen for content maximum in paper waste has good Degradation.Ehter bond in these enzymes attack simultaneously lignin structures, C ?C key and C α ?the two keys of C β, lignin structure is destroyed rapidly, generate minute lower materials such as aromatics (as Vanillin, vanillic acid and quinones substance), muconate and part small molecules carboxylic acid (formic acid) of quantum.These organic acid generations can make the pH value of system reduce gradually, thereby suppress the growth metabolism of above-mentioned three kinds of bacterium, cause enzymic activity to reduce.The pseudomonas putida of adding and enterobacter cloacae can utilize the small molecular organic acid that in paper waste, larger molecular organics degraded generates to carry out growth metabolism fast, and be further degraded into the inorganicss such as carbonic acid gas and water, the pH value of system is restored, thereby restraining effect is eliminated, reaches the object that continues COD, BOD and SS in the paper waste of efficient place to go.Due to the complementary and synergy between variant bacterial strain, their combined action just have very high degradation rate to the organism in paper waste.
Accompanying drawing explanation
Fig. 1 is five kinds of growing state figure that thalline is cultivated at aseptic proliferated culture medium in embodiment 1.
Fig. 2 is five kinds of growing state figure that thalline is cultivated at aseptic proliferated culture medium in embodiment 2.
Fig. 3 is five kinds of growing state figure that thalline is cultivated at aseptic proliferated culture medium in embodiment 3.
Embodiment
Below in conjunction with drawings and Examples, the present invention is further illustrated, but the scope of protection of present invention is not limited to the scope of embodiment statement.
After waste wash liquor in embodiment after pulping and paper-making National Key Laboratory of paper waste Wei Nan Polytechnics employing import Eucalyptus sulphate process displacement cooking dilutes 10 times, make, this digesting technoloy alkali charge is 21%, and upper extracting alkali charge accounts for 60% of total alkali charge.Test the COD of waste water used
crbe 1510～1680mg/L, colourity is 2360～2425C.U, and SS is 38～49mg/L, pH regulator to 7.0～9.0.The solution that wherein dilutes waste water is aseptic minimal medium, and its main component is CaCl
20.01～0.02g/L, KH
2pO
42.0～2.5g/L, MgSO
40.25～0.30g/L, ammonium tartrate 0.5～0.6g/L, NH
4nO
30.5～0.6g/L, all the other are water.
Embodiment 1:
Difference picking edaphic bacillus (Agrobacterium sp.), rod bacterium (Bacillus sp.), enterobacteria (Enterobacter.cloacae.), pseudomonas putida (Pseudomonas putida.), five kinds of bacterium 1 rings of Pseudomonas stutzeri (Pseudomonas stutzeri.) (from the mud of the water port in paper mill separated obtain): it is transferred to respectively to (its composition is 1.5g/L extractum carnis containing 20mL nutritive medium, 1.0g/L glucose, 5.5g/L Tryptones, 3.0g/L yeast powder, pH6.5, all the other are water) container in, every kind of bacterium is cultivated 2 days under the condition of 27 ℃, again the volume ratio (thalline and proliferated culture medium) of five kinds of bacterium difference 1:9 after cultivating is seeded in the container containing 300mL proliferated culture medium, under the condition of 27 ℃, cultivate 2 days, after the final centrifugal 15min of speed with 6000rpm, obtain respectively the logarithmic phase cell of above-mentioned five kinds of thalline, with after normal saline flushing, dry refrigeration is standby.Wherein, five kinds of different bacteriums adopt different proliferated culture mediums, and the nutritional medium of edaphic bacillus, rod bacterium and enterobacter cloacae is extractum carnis 1.5g/L, glucose 1.0g/L, and Tryptones 6.0g/L, yeast powder 3.0g/L, all the other are water; Pseudomonas stutzeri and pseudomonas putida nutritional medium are fresh potato juice 800mL, glucose 16g, and all the other are water.Suitably cultivate the growing state of rear five kinds of bacteriums as shown in Figure 1.As can be seen from Figure 1, the growth curve of five kinds of bacteriums all obviously presents lag phase, logarithmic phase and stationary phase, but different strain reaches required Time Inconsistency in each period.Edaphic bacillus (Agrobacterium sp.) enters logarithmic phase at cultivation 4h, after 20h, enter stationary phase, rod bacterium (Bacillus sp.) enters logarithmic phase at cultivation 2h, after 20h, enter stationary phase, enterobacteria (Enterobacter.Cloacae.) enters logarithmic phase at cultivation 1h, after 4h, enter stationary phase, pseudomonas putida (Pseudomonas putida) just enters logarithmic phase after cultivating 6h, 12h enters stationary phase, Pseudomonas stutzeri (Pseudomonas stutzeri.) just enters logarithmic phase after cultivating 2h, 7h enters stationary phase.
The logarithmic phase cell of above-mentioned five kinds of thalline is taken out, with phosphate buffered saline buffer (its main component sodium-chlor 8.0g/L, Repone K 0.2g/L, dipotassium hydrogen phosphate 1.1g/L and potassium primary phosphate 0.2g/L, all the other are water) wash after 2 times, per-cent meter by volume, get respectively 5% edaphic bacillus (Agrobacterium sp.), 3% rod bacterium (Bacillus sp.), 13% enterobacter cloacae (Enterobacter cloacae), 41% pseudomonas putida (Pseudomonas putida) and 38% Pseudomonas stutzeri (Pseudomonas stutzeri.) mix, and be suspended in physiological saline, refrigerate standby.
Get mixed bacteria liquid obtained above and with the volume ratio (mixed bacteria liquid and nutritive medium) of 1:20, add nutritive medium and cultivate 6h, make directly to put in 1L paper waste after its activation, Air Exposure 2d, aeration rate is 2L/h.In mass concentration, the composition of nutritive medium is 1.5g/L extractum carnis, 1.0g/L glucose, and 5.5g/L Tryptones, 3.0g/L yeast powder, pH6.5, all the other are water.
Pulping and paper-making National Key Laboratory of above-mentioned paper waste Wei Nan Polytechnics makes after adopting 10 times of waste wash liquor dilutions after import Eucalyptus sulphate process displacement cooking, and this digesting technoloy alkali charge is 21%, and upper extracting alkali charge accounts for 60% of total alkali charge.Test the COD of waste water used
crfor 1510mg/L, colourity is 2360C.U, and SS is 41mg/L, pH regulator to 7.0.The solution that wherein dilutes waste water is aseptic minimal medium, and its main component is CaCl
20.01g/L, KH
2pO
42.0g/L, MgSO
40.25g/L, ammonium tartrate 0.5g/L, NH
4nO
30.5g/L, all the other are water.
Adopt the present embodiment method to process above-mentioned paper waste, COD after Air Exposure 2d
cr, colourity and SS clearance as shown in table 1.
Table 1 experimental result
The result of table 1 show composite flora to the treatment effect of paper waste significantly better than contrast with add single culture separately.Existence between each bacterial classification of composite flora interacts, interaction energy between bacterial classification impels bacterium to produce involved enzyme of more extracellular polymeric, these enzymes and cell extracellular polymeric have stronger Degradation to the organism in waste water and have absorption, throwing out concurrently, contribute to organic removal in paper waste.
Embodiment 2
Five kinds of bacterium of picking 2 encircle respectively: edaphic bacillus (Agrobacterium sp.), rod bacterium (Bacillus sp.), enterobacteria (Enterobacter.Cloacae.), pseudomonas putida (Pseudomonas putida.), Pseudomonas stutzeri (Pseudomonas stutzeri.) (from the mud of the water port in paper mill separated obtain) is transferred to it respectively that (its composition is 2.0g/L extractum carnis containing 30mL nutritive medium, 1.5g/L glucose, 6.5g/L Tryptones, 3.5g/L yeast powder, pH7.5, all the other are water) container in, every kind of bacterium is cultivated 2 days under the condition of 30 ℃, again five kinds of bacterium after cultivating are seeded in the container containing 500mL proliferated culture medium with the volume ratio (thalline and proliferated culture medium) of 1:10 respectively, under the condition of 35 ℃, cultivate 1 day, after the final centrifugal 10min of speed with 7000rpm, obtain respectively the logarithmic phase cell of above-mentioned five kinds of thalline, with after normal saline flushing, dry refrigeration is standby.Wherein, five kinds of different bacteriums adopt different proliferated culture mediums, and the nutritional medium of edaphic bacillus, rod bacterium and enterobacter cloacae is extractum carnis 2.0g/L, glucose 1.5g/L, and Tryptones 7.0g/L, yeast powder 3.5g/L, all the other are water; Pseudomonas stutzeri and pseudomonas putida nutritional medium are fresh potato juice 900mL, glucose 20g, and all the other are water.Suitably cultivate the growing state of rear five kinds of bacteriums as shown in Figure 2.As can be seen from Figure 2, the growth curve of five kinds of bacteriums all obviously presents lag phase, logarithmic phase and stationary phase, but different strain reaches required Time Inconsistency in each period.Edaphic bacillus (Agrobacterium sp.) enters logarithmic phase at cultivation 2h, after 15h, enter stationary phase, rod bacterium (Bacillus sp.) enters logarithmic phase at cultivation 2h, after 18h, enter stationary phase, enterobacteria (Enterobacter.Cloacae.) enters logarithmic phase at cultivation 1h, after 6h, enter stationary phase, after 5h, just enter stationary phase, pseudomonas putida (Pseudomonas putida) just enters logarithmic phase after cultivating 3h, 14h enters stationary phase, Pseudomonas stutzeri (Pseudomonas stutzeri.) just enters logarithmic phase after cultivating 5h, 12h enters stationary phase.
The logarithmic phase cell of above-mentioned five kinds of thalline is taken out, with phosphate buffered saline buffer (its main component sodium-chlor 9.0g/L, Repone K 0.25g/L, dipotassium hydrogen phosphate 1.2g/L and potassium primary phosphate 0.25g/L) wash after 3 times, per-cent meter by volume, get respectively 6% edaphic bacillus (Agrobacterium sp.), 2% rod bacterium (Bacillus sp.), 10% enterobacter cloacae (Enterobacter cloacae), 26% pseudomonas putida (Pseudomonas putida) and 56% Pseudomonas stutzeri (Pseudomonas stutzeri.) mix and are suspended in physiological saline, refrigerate standby.
Get mixed bacteria liquid obtained above and add to nutritive medium and cultivate 8h with the volume ratio (mixed bacteria liquid and nutritive medium) of 1:15, make directly to put in 1L paper waste after its activation, Air Exposure 4d, aeration rate is 4L/h.In mass concentration, the composition of nutritive medium is 2.0g/L extractum carnis, 1.5g/L glucose, and 6.5g/L Tryptones, 3.5g/L yeast powder, pH7.5, all the other are water.
Pulping and paper-making National Key Laboratory of above-mentioned paper waste Wei Nan Polytechnics makes after adopting 10 times of waste wash liquor dilutions after import Eucalyptus sulphate process displacement cooking, and this digesting technoloy alkali charge is 21%, and upper extracting alkali charge accounts for 60% of total alkali charge.Test the COD of waste water used
crfor 1690mg/L, colourity is 2400C.U, and SS is 38mg/L, pH regulator to 9.0.The solution that wherein dilutes waste water is aseptic minimal medium, and its main component is CaCl
20.02g/L, KH
2pO
42.5g/L, MgSO
40.27g/L, ammonium tartrate 0.6g/L, NH
4nO
30.6g/L, all the other are water.
Adopt the present embodiment method to process above-mentioned paper waste, COD after Air Exposure 4d
cr, colourity and SS clearance as shown in table 2.
Table 2 experimental result
The result of table 2 show composite flora to the treatment effect of paper waste significantly better than contrast with add single culture separately.Existence between each bacterial classification of composite flora interacts, interaction energy between bacterial classification impels bacterium to produce involved enzyme of more extracellular polymeric, these enzymes and cell extracellular polymeric have stronger Degradation to the organism in waste water and have absorption, throwing out concurrently, contribute to organic removal in paper waste.
Embodiment 3
Five kinds of bacterium of picking 2 encircle respectively: edaphic bacillus (Agrobacterium sp.), rod bacterium (Bacillus sp.), enterobacteria (Enterobacter.Cloacae.), pseudomonas putida (Pseudomonas putida.), Pseudomonas stutzeri (Pseudomonas stutzeri.) (from the mud of the water port in paper mill separated obtain) is transferred to it respectively that (its composition is 1.7g/L extractum carnis containing 25mL nutritive medium, 2.0g/L glucose, 6.0g/L Tryptones, 4.0g/L yeast powder, pH7.0, all the other are water) container in, every kind of bacterium is cultivated 2 days under the condition of 30 ℃, again five kinds of bacterium after cultivating are seeded in the container containing 400mL proliferated culture medium with the volume ratio (thalline and proliferated culture medium) of 1:12 respectively, under the condition of 35 ℃, cultivate 2 days, after the final centrifugal 10min of speed with 7000rpm, obtain respectively the logarithmic phase cell of above-mentioned five kinds of thalline, with after normal saline flushing, dry refrigeration is standby.Wherein, five kinds of different bacteriums adopt different proliferated culture mediums, and wherein the nutritional medium of edaphic bacillus, rod bacterium and enterobacter cloacae is extractum carnis 1.7g/L, glucose 2.0g/L, and Tryptones 6.5g/L, yeast powder 4.0g/L, all the other are water; Fresh potato juice 1000mL, glucose 18g, all the other are water.Suitably cultivate the growing state of rear five kinds of bacteriums as shown in Figure 3.As can be seen from Figure 3, the growth curve of five kinds of bacteriums all obviously presents lag phase, logarithmic phase and stationary phase, but different strain reaches required Time Inconsistency in each period.Edaphic bacillus (Agrobacterium sp.) enters logarithmic phase at cultivation 5h, after 18h, enter stationary phase, rod bacterium (Bacillus sp.) enters logarithmic phase at cultivation 2h, after 20h, enter stationary phase, enterobacteria (Enterobacter.Cloacae.) enters logarithmic phase at cultivation 1h, after 7h, enter stationary phase, pseudomonas putida (Pseudomonas putida) just enters logarithmic phase after cultivating 6h, 14h enters stationary phase, Pseudomonas stutzeri (Pseudomonas stutzeri.) just enters logarithmic phase after cultivating 2h, 10h enters stationary phase.
The logarithmic phase cell of above-mentioned five kinds of thalline is taken out, with phosphate buffered saline buffer, (its main component is sodium-chlor 8.5g/L, Repone K 0.3g/L, dipotassium hydrogen phosphate 1.15g/L and potassium primary phosphate 0.3g/L, all the other are water) wash after 3 times, per-cent meter by volume, get respectively 8% edaphic bacillus (Agrobacterium sp.), 5% rod bacterium (Bacillus sp.), 15% enterobacter cloacae (Enterobacter cloacae), 38% pseudomonas putida (Pseudomonas putida) and 34% Pseudomonas stutzeri (Pseudomonas stutzeri.) mix and are suspended in physiological saline, refrigerate standby.
Get mixed bacteria liquid obtained above and add to nutritive medium and cultivate 12h with the volume ratio (mixed bacteria liquid and nutritive medium) of 1:20, make directly to put in 1L paper waste after its activation, Air Exposure 3d, aeration rate is 3L/h.In mass concentration, the composition of nutritive medium is 1.7g/L extractum carnis, 2.0g/L glucose, and 6.0g/L Tryptones, 4.0g/L yeast powder, pH7.0, all the other are water.
Pulping and paper-making National Key Laboratory of above-mentioned paper waste Wei Nan Polytechnics makes after adopting 10 times of waste wash liquor dilutions after import Eucalyptus sulphate process displacement cooking, and this digesting technoloy alkali charge is 21%, and upper extracting alkali charge accounts for 60% of total alkali charge.Test the COD of waste water used
crfor 1680mg/L, colourity is 2425C.U, and SS is 49mg/L, pH regulator to 8.0.The solution that wherein dilutes waste water is aseptic minimal medium, and its main component is CaCl
20.015g/L, KH
2pO
42.3g/L, MgSO
40.30g/L, ammonium tartrate 0.55g/L, NH
4nO
30.55g/L, all the other are water.
Adopt the present embodiment method to process above-mentioned paper waste, COD after Air Exposure 4d
cr, colourity and SS clearance as shown in table 3.
Table 3 experimental result
The result of table 3 show composite flora to the treatment effect of paper waste significantly better than contrast with add single culture separately.Existence between each bacterial classification of composite flora interacts, interaction energy between bacterial classification impels bacterium to produce involved enzyme of more extracellular polymeric, these enzymes and cell extracellular polymeric have stronger Degradation to the organism in waste water and have absorption, throwing out concurrently, contribute to organic removal in paper waste.
Above-described embodiment is preferably embodiment of the present invention; but embodiments of the present invention are not restricted to the described embodiments; other any do not deviate from change, the modification done under spirit of the present invention and principle, substitutes, combination, simplify; all should be equivalent substitute mode, within being included in protection scope of the present invention.
In the present invention, the microflora forming by optimum combination can realize the collaborative and complementary action of five kinds of microorganisms in system, overcome single culture to the inefficient problem of organic matter degradation in high density paper waste, made whole system all there is efficient degradation capability to paper waste.COD wherein
crclearance is 62～75%, and chroma removal rate is that 81～87%, SS clearance is 77～87%.According to related microorganisms, administer the report of paper waste: Wu etc. and process pulping with high density paper waste with Pleurotus ostreatus, maximum COD clearance is 48%[Juan Wu, Yazhong Xiao, Hanqing Yu.Degradation of lignin in pulp mill wastewaters by white ?rot fungi on biofilm.Bioresource Technology96 (2005) 1357 – 1363].These are starkly lower than composite flora of the present invention with single bacterium to the treatment effect of paper waste, so this composite flora is having a good application prospect aspect processing xylogen waste water.
Claims (6)
1. for a preparation method for the composite flora of the paper waste of degrading, it is characterized in that, comprise the steps:
The preparation of (1) five kind of bacterium logarithmic phase cell: difference picking edaphic bacillus (Agrobacterium sp.), rod bacterium (Bacillus sp.), enterobacter cloacae (Enterobacter.cloacae.), pseudomonas putida (Pseudomonas putida.), five kinds of bacterium of Pseudomonas stutzeri (Pseudomonas stutzeri.), 1～2 ring, it is transferred to respectively containing in 20～50mL nutritive medium, every kind of bacterium is cultivated 1～2 day under the condition of 27～35 ℃, again five kinds of bacterium after cultivating are seeded to containing in the container of 300～500mL proliferated culture medium with the volume ratio of 1:9～1:12 respectively by thalline and proliferated culture medium volume ratio, adopt different proliferated culture mediums to cultivate, wherein, the nutritional medium of edaphic bacillus, rod bacterium and enterobacter cloacae is extractum carnis 1.5g～2.0/L, glucose 1.0～2.0g/L, and Tryptones 6.0～7.0g/L, yeast powder 3.0～4.0g/L, all the other are water, Pseudomonas stutzeri and pseudomonas putida nutritional medium are fresh potato juice 800～1000mL, glucose 16～20g, all the other are the water (preparation method of potato juice: remove 160～200 grams of skin fresh potatos, be cut into small pieces, adding deionized water 800～1000mL boils 30 minutes, elimination potato ball, complements to 800～1000mL with deionized water by filtrate), then under the condition of 27～35 ℃, cultivate 1～2 day, after the centrifugal 10～15min of speed with 6000～7000rpm, obtain respectively the logarithmic phase cell of above-mentioned five kinds of thalline, described nutritive medium main component is extractum carnis 1.5～2.0g/L, glucose 1.0～2.0g/L, and Tryptones 5.5～6.5g/L, yeast powder 3.0～4.0g/L, pH6.5～7.5, all the other are water,
The mixed bacterial of (2) five kinds of thalline composite: the logarithmic phase cell of described five kinds of thalline is taken out, with after phosphate buffered saline buffer washing 2～3 times, per-cent meter by volume, get respectively 5～8% edaphic bacilluss (Agrobacterium sp.), 2～5% rod bacteriums (Bacillus sp.), 10～15% enterobacter cloacaes (Enterobacter cloacae), 26～41% pseudomonas putidas (Pseudomonas putida) and 34～56% Pseudomonas stutzeris (Pseudomonas stutzeri.) mix; Must be for the composite flora of the paper waste of degrading.
2. the preparation method of the composite flora for the paper waste of degrading according to claim 1, it is characterized in that, during described composite flora degraded paper waste, the mixed bacteria liquid of getting composite flora is added to and in nutritive medium, cultivates 6～12h with the volume ratio of 1:15～1:30, make directly to put in paper waste after composite flora activation, every 1 liter of paper waste adds mixed bacteria liquid 1～2mL; Air Exposure 2～4d, aeration rate is 2～4L/h.
3. the preparation method of the composite flora for the paper waste of degrading according to claim 2, it is characterized in that, described nutritive medium main component is extractum carnis 1.5～2.0g/L, glucose 1.0～2.0g/L, Tryptones 5.5～6.5g/L, yeast powder 3.0～4.0g/L, pH6.5～7.5, all the other are water.
4. the preparation method of the composite flora for the paper waste of degrading according to claim 1, it is characterized in that, per-cent meter by volume, the composition of described phosphate buffered saline buffer is sodium-chlor 8.0～9.0g/L, Repone K 0.2～0.3g/L, dipotassium hydrogen phosphate 1.1～1.2g/L and potassium primary phosphate 0.2～0.3g/L, all the other are water.
5. according to the preparation method of the composite flora for the paper waste of degrading described in claim 1 or 3, it is characterized in that, the washing times of described phosphate buffered saline buffer is 2～3 times.
6. for a composite flora for the paper waste of degrading, it is characterized in that, by preparation method described in claim 1,4 or 5, made.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754855A (en) * | 2016-06-29 | 2017-05-31 | 华南理工大学 | Embedding type Nanoscale Iron/complex micro organism fungicide and preparation method thereof |
| CN107475150A (en) * | 2017-08-24 | 2017-12-15 | 青岛理工大学 | Compound flora for decolorizing papermaking industrial wastewater and preparation method thereof |
| WO2019079795A1 (en) * | 2017-10-20 | 2019-04-25 | BiOWiSH Technologies, Inc. | Compositions and methods for reducing cyanuric acid in recreational water systems |
| CN110713950A (en) * | 2019-11-07 | 2020-01-21 | 云南嘉谷环保有限公司 | Organic wastewater composite microbial treatment agent and use method thereof |
| CN111662851A (en) * | 2020-07-14 | 2020-09-15 | 南京工业大学 | Bacterial strain for oxidizing aromatic compounds and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102373191A (en) * | 2011-09-26 | 2012-03-14 | 华南理工大学 | Dominant consortium flora for degrading urban waste water and preparation method thereof |
| CN102390889A (en) * | 2011-09-26 | 2012-03-28 | 华南理工大学 | Method for treating pulping and papermaking wastewater by using dominant degradation bacteria |
-
2013
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102373191A (en) * | 2011-09-26 | 2012-03-14 | 华南理工大学 | Dominant consortium flora for degrading urban waste water and preparation method thereof |
| CN102390889A (en) * | 2011-09-26 | 2012-03-28 | 华南理工大学 | Method for treating pulping and papermaking wastewater by using dominant degradation bacteria |
Non-Patent Citations (2)
| Title |
|---|
| HONGLEI CHEN ET AL: "Enhanced biodegradation of pulping effluents by a statistical experimental design using microbial consortia", 《BIORESOURCES》, vol. 5, no. 3, 31 December 2010 (2010-12-31) * |
| 陈洪雷 等: "构建优势菌群提高好氧污泥对制浆废水的处理效果", 《造纸科学与技术》, vol. 31, no. 2, 15 April 2012 (2012-04-15) * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106754855A (en) * | 2016-06-29 | 2017-05-31 | 华南理工大学 | Embedding type Nanoscale Iron/complex micro organism fungicide and preparation method thereof |
| CN106754855B (en) * | 2016-06-29 | 2020-07-28 | 华南理工大学 | Embedded nano-iron/compound microbial agent and preparation method thereof |
| CN107475150A (en) * | 2017-08-24 | 2017-12-15 | 青岛理工大学 | Compound flora for decolorizing papermaking industrial wastewater and preparation method thereof |
| WO2019079795A1 (en) * | 2017-10-20 | 2019-04-25 | BiOWiSH Technologies, Inc. | Compositions and methods for reducing cyanuric acid in recreational water systems |
| CN110713950A (en) * | 2019-11-07 | 2020-01-21 | 云南嘉谷环保有限公司 | Organic wastewater composite microbial treatment agent and use method thereof |
| CN111662851A (en) * | 2020-07-14 | 2020-09-15 | 南京工业大学 | Bacterial strain for oxidizing aromatic compounds and application thereof |
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