CN107475150A - Compound flora for decolorizing papermaking industrial wastewater and preparation method thereof - Google Patents
Compound flora for decolorizing papermaking industrial wastewater and preparation method thereof Download PDFInfo
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- CN107475150A CN107475150A CN201710735660.8A CN201710735660A CN107475150A CN 107475150 A CN107475150 A CN 107475150A CN 201710735660 A CN201710735660 A CN 201710735660A CN 107475150 A CN107475150 A CN 107475150A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 7
- 239000010842 industrial wastewater Substances 0.000 title claims description 7
- 150000001875 compounds Chemical class 0.000 title 1
- 241000894006 Bacteria Species 0.000 claims abstract description 21
- 241000588697 Enterobacter cloacae Species 0.000 claims abstract description 15
- 241000589776 Pseudomonas putida Species 0.000 claims abstract description 15
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 14
- 241000589614 Pseudomonas stutzeri Species 0.000 claims abstract description 13
- 241000233866 Fungi Species 0.000 claims abstract description 12
- 241000194103 Bacillus pumilus Species 0.000 claims abstract description 11
- 239000002131 composite material Substances 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 239000002253 acid Substances 0.000 claims description 12
- 241000589158 Agrobacterium Species 0.000 claims description 11
- 239000001963 growth medium Substances 0.000 claims description 11
- 244000061456 Solanum tuberosum Species 0.000 claims description 8
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 8
- 239000002609 medium Substances 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 5
- 239000000725 suspension Substances 0.000 claims description 5
- 238000012360 testing method Methods 0.000 claims description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 4
- 239000003876 biosurfactant Substances 0.000 claims description 4
- 239000008367 deionised water Substances 0.000 claims description 4
- 229910021641 deionized water Inorganic materials 0.000 claims description 4
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 4
- 239000008103 glucose Substances 0.000 claims description 4
- 235000015097 nutrients Nutrition 0.000 claims description 4
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 2
- 235000015278 beef Nutrition 0.000 claims description 2
- 239000000706 filtrate Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 239000012137 tryptone Substances 0.000 claims description 2
- 239000006071 cream Substances 0.000 claims 1
- 244000005700 microbiome Species 0.000 abstract description 10
- 230000015556 catabolic process Effects 0.000 abstract description 6
- 238000006731 degradation reaction Methods 0.000 abstract description 6
- 239000002351 wastewater Substances 0.000 abstract description 5
- 230000003993 interaction Effects 0.000 abstract description 4
- 239000003344 environmental pollutant Substances 0.000 abstract description 3
- 239000013067 intermediate product Substances 0.000 abstract description 3
- 231100000719 pollutant Toxicity 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 239000002207 metabolite Substances 0.000 abstract description 2
- 241000589155 Agrobacterium tumefaciens Species 0.000 abstract 1
- 241000520272 Pantoea Species 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 description 11
- 239000002585 base Substances 0.000 description 9
- 239000003795 chemical substances by application Substances 0.000 description 9
- 230000001580 bacterial effect Effects 0.000 description 8
- 239000000126 substance Substances 0.000 description 5
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 4
- 239000003513 alkali Substances 0.000 description 4
- 239000000356 contaminant Substances 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 239000000843 powder Substances 0.000 description 4
- 239000010902 straw Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000004537 pulping Methods 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 239000010893 paper waste Substances 0.000 description 2
- 244000166124 Eucalyptus globulus Species 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- NGPGDYLVALNKEG-UHFFFAOYSA-N azanium;azane;2,3,4-trihydroxy-4-oxobutanoate Chemical compound [NH4+].[NH4+].[O-]C(=O)C(O)C(O)C([O-])=O NGPGDYLVALNKEG-UHFFFAOYSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 238000004061 bleaching Methods 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010411 cooking Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000006385 ozonation reaction Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/26—Nature of the water, waste water, sewage or sludge to be treated from the processing of plants or parts thereof
- C02F2103/28—Nature of the water, waste water, sewage or sludge to be treated from the processing of plants or parts thereof from the paper or cellulose industry
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Environmental & Geological Engineering (AREA)
- Biodiversity & Conservation Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Water Supply & Treatment (AREA)
- Biomedical Technology (AREA)
- Hydrology & Water Resources (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a composite flora for decolorizing paper industry wastewater and a preparation method thereof, wherein the composite flora comprises 2-10 parts of bacillus pantoea, 10-20 parts of bacillus pumilus wilting, 20-30 parts of white rot fungi, 10-15 parts of pseudomonas putida, 20-30 parts of agrobacterium tumefaciens, 10-30 parts of pseudomonas stutzeri and 20-30 parts of enterobacter cloacae. The invention utilizes the beneficial interaction among microorganisms, cultures and acclimates the microorganisms in a mixing way, and improves the flow direction of the bacteria to the intermediate metabolite of the pollutants to ensure that the inhibitory intermediate product is not generated or is converted as soon as possible, thereby improving the degradation efficiency of the pollutants.
Description
Technical field
The present invention relates to pulping wastewater treatment field, and in particular to a kind of composite flora that paper-making industrial waste water decolourizes and its
Preparation method.
Background technology
China's paper industry is still high consumption, inefficient, heavily contaminated with aqueous condition, with China water resources condition pole not
Adapt to, and one of severe challenge for being faced at present of paper industry.After the technologies such as alkali collection, biological treatment are employed, shadow
Ring paper waste and reach the problem of one of key of New emission standard or reuse is colourity.
Because the main component for causing chromaticity of paper-making waste water is the chromonic material based on lignin, its chemical stability
It is good, it is difficult to remove.Traditional method is more to be decolourized with strong oxidizers such as hydrogen peroxide or Fenton reagents, costly and chemical substance
Secondary pollution caused by addition is the major defect of chemical method.The adsorption bleaching such as activated carbon or carclazyte can consume substantial amounts of absorption
Agent, it can also form the problems such as secondary pollution is disposed.Ozone is used for the processing of the color development waste water of pulping and paper-making, is not required to residual in water
Other chemical substances are stayed, the water quality after processing is good, and operation is simple to operate.But the mistake of ozonation technology processing industrial wastewater
Cheng Zhong, some shortcomings are still had, be specially：(1) due to the reasons such as ozone itself decomposition, dissolving utilization rate, ozone utilization rate
It is not high；(2) selectivity of ozone oxidation is stronger, and it is relatively low to hardly degraded organic substance clearance, it is necessary to using catalyst, causes smelly
Oxygen oxidation dosage is big, adds operating cost.
The content of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide, a kind of decolorizing efficiency is high, operating cost is low makes
Composite flora that paper industrial wastewater decolourizes and preparation method thereof.
The purpose of the present invention is achieved through the following technical solutions：
The technical scheme is that：
The composite flora that a kind of paper-making industrial waste water decolourizes, wilted by the general branch acid bacillus of 2-10 parts, 10-20 parts short and small
Bacillus, 20-30 parts white-rot fungi, 10-15 parts pseudomonas putida, 20-30 parts agrobacterium, 10-30 parts Pseudomonas stutzeri,
20-30 parts enterobacter cloacae forms；Wherein, agrobacterium and the nutrient medium of enterobacter cloacae are beef extract 1.5-2.0g/
L, glucose 1.0-2.0g/L, tryptone 6.0-7.0g/L, dusty yeast 3.0-4.0g/L, remaining is water；Pseudomonas stutzeri
It is fresh potato juice 800-1000mL, glucose 16-20g with pseudomonas putida nutrient medium, remaining is water；Potato
The preparation method of juice：Remove skin fresh potato 160；200 grams, it is cut into small pieces, adds deionized water 800；1000mL, boil 30 points
Clock, potato ball is filtered off, filtrate is complemented to 800,1000mL with deionized water.
The present invention also provides the method for preparing above-mentioned composite flora, and its step is：
1) actication of culture：It is true using the general branch acid bacillus of 2-10 parts, 10-20 part wiltings bacillus pumilis, 20-30 part white rots
Bacterium, 10-15 parts pseudomonas putida, 20-30 parts agrobacterium, 10-30 parts Pseudomonas stutzeri, 20-30 part enterobacter cloacaes,
The strain is inoculated on the slant medium in test tube, cultivated 3-7 days respectively at 25-30 DEG C；
2) Shaking culture：Bacteria suspension is made with sterilized water in above-mentioned slant culture, then by 10-15 part Pseudomonas putidas
Bacterium, 20-30 parts agrobacterium, 10-30 parts Pseudomonas stutzeri, 20-30 part enterobacter cloacaes, which are inoculated into, produces biological surface work
Cultivate in property agent culture medium, cultivated 3~7 days at 25~30 DEG C respectively；The general branch acid bacillus of 2-10 parts, 10-20 parts are wilted
Bacillus pumilis, 20-30 part white-rot fungis are inoculated into bacteria culture media and cultivated respectively, are cultivated 3~7 days at 25~30 DEG C；
3) mix：Step 2) the microbial fermentation solution is mixed in the microorganism weight in wet base ratio, while adds micro- life
The powder of straw of thing microbial inoculum weight in wet base total amount 20~30% obtains microbial bacterial agent as microbe carrier.
The invention has the advantages that：
Present invention discover that the degradation effect of composite flora is far above any one plant of single bacterial strain, illustrate between different microorganisms
Produce certain interaction.This is due to that many biological activities are that individual plant Institute of Micro-biology can not complete or faint can only carry out
, it is necessary to realized by interaction of two or more microorganisms in same environment.Using single flora and gene work
During journey bacterium carries out contaminant degradation, usually the generation due to inhibition mesostate or intermediate product enter cut-off type
Metabolite approach and inhibit contaminant degradation enzymatic activity so that contaminant degradation it is inefficient or degraded it is not thorough.Utilize
Beneficial interaction between microorganism, microorganism is mixed and tamed, the mesostate stream by bacterium to pollutant
To some improvement are carried out, inhibition intermediate product is set not generate or convert as early as possible, so as to improve contaminant degradation efficiency.
Embodiment
Paper waste is that southern Polytechnics's pulping and paper-making National Key Laboratory uses import Eucalyptus sulfate in embodiment
Waste wash liquor after method displacement cooking is made after diluting 10 times, and the digesting technoloy alkali charge is 21%, and upper extracting alkali charge accounts for always
The 60% of alkali charge.The CODCr of waste water used in experiment is 1510~1680mg/L, and colourity is 2360~2425C.U, SS is 38~
49mg/L, pH are adjusted to 7.0~9.0.The solution for wherein diluting waste water is sterile minimal medium, and its main component is
CaCl20.01~0.02g/L, KH2PO42.0~2.5g/L, MgSO40.25~0.30g/L, 0.5~0.6g/L of ammonium tartrate,
NH4NO30.5~0.6g/L, remaining is water.
Embodiment 1：
1) 10 parts of general branch acid bacillus, 10 parts of wilting bacillus pumilises, 20 parts of white-rot fungis, 15 parts of Pseudomonas putidas are used
Bacterium, 30 parts of agrobacteriums, 10 parts of Pseudomonas stutzeris, 30 parts of enterobacter cloacaes, the inclined-plane strain being inoculated into test tube
On culture medium, cultivated 3 days respectively at 30 DEG C；
2) bacteria suspension is made with sterilized water in above-mentioned slant culture, then by 15 parts of pseudomonas putidas, 30 parts of soil bars
Bacterium, 10 parts of Pseudomonas stutzeris, 30 parts of enterobacter cloacaes are inoculated into produce and cultivated respectively in biosurfactant culture medium,
Cultivated 3 days at 30 DEG C；10 parts of general branch acid bacillus, 10 parts of wilting bacillus pumilises, 20 parts of white-rot fungis are inoculated into Bacteria Culture
Cultivate in base, cultivated 3 days at 30 DEG C respectively；
3) the step 2) microbial fermentation solution is mixed in the microorganism weight in wet base ratio, while adds microbial bacterial agent
The powder of straw of weight in wet base total amount 20% obtains microbial bacterial agent as microbe carrier.
Embodiment 2：
1) 2 parts of general branch acid bacillus, 10 parts of wilting bacillus pumilises, 30 parts of white-rot fungis, 10 parts of Pseudomonas putidas are used
Bacterium, 20 parts of agrobacteriums, 10 parts of Pseudomonas stutzeris, 20 parts of enterobacter cloacaes, the inclined-plane strain being inoculated into test tube
On culture medium, cultivated 7 days respectively at 25 DEG C；
2) bacteria suspension is made with sterilized water in above-mentioned slant culture, then by 10 parts of pseudomonas putidas, 20 parts of soil bars
Bacterium, 10 parts of Pseudomonas stutzeris, 20 parts of enterobacter cloacaes are inoculated into produce and cultivated respectively in biosurfactant culture medium,
Cultivated 7 days at 25 DEG C；2 parts of general branch acid bacillus, 10 parts of wilting bacillus pumilises, 30 parts of white-rot fungis are inoculated into bacteria culture media
It is middle to cultivate respectively, cultivated 7 days at 25 DEG C；
3) the step 2) microbial fermentation solution is mixed in the microorganism weight in wet base ratio, while adds microbial bacterial agent
The powder of straw of weight in wet base total amount 30% obtains microbial bacterial agent as microbe carrier.
Embodiment 3：
1) 8 parts of general branch acid bacillus, 15 parts of wilting bacillus pumilises, 25 parts of white-rot fungis, 10 parts of Pseudomonas putidas are used
Bacterium, 25 parts of agrobacteriums, 20 parts of Pseudomonas stutzeris, 25 parts of enterobacter cloacaes, the inclined-plane strain being inoculated into test tube
On culture medium, cultivated respectively at 25 DEG C 5 days；
2) bacteria suspension is made with sterilized water in above-mentioned slant culture, then by 10 parts of pseudomonas putidas, 25 parts of soil bars
Bacterium, 20 parts of Pseudomonas stutzeris, 25 parts of enterobacter cloacaes are inoculated into produce and cultivated respectively in biosurfactant culture medium,
Cultivated 5 days at 25 DEG C；8 parts of general branch acid bacillus, 15 parts of wilting bacillus pumilises, 25 parts of white-rot fungis are inoculated into bacteria culture media
It is middle to cultivate respectively, cultivated 5 days at 25 DEG C；
3) the step 2) microbial fermentation solution is mixed in the microorganism weight in wet base ratio, while adds microbial bacterial agent
The powder of straw of weight in wet base total amount 30% obtains microbial bacterial agent as microbe carrier.
Claims (2)
- A kind of 1. composite flora that paper-making industrial waste water decolourizes, it is characterised in that：By the general branch acid bacillus of 2-10 parts, 10-20 Part wilting bacillus pumilis, 20-30 parts white-rot fungi, 10-15 parts pseudomonas putida, 20-30 parts agrobacterium, 10-30 parts are applied Family name pseudomonad, 20-30 parts enterobacter cloacae composition；Wherein, agrobacterium and the nutrient medium of enterobacter cloacae are beef Cream 1.5-2.0g/L, glucose 1.0-2.0g/L, tryptone 6.0-7.0g/L, dusty yeast 3.0-4.0g/L, remaining is water；Apply Family name pseudomonad and pseudomonas putida nutrient medium are fresh potato juice 800-1000mL, glucose 16-20g, remaining For water；The preparation method of potato juice：Remove skin fresh potato 160；200 grams, it is cut into small pieces, adds deionized water 800； 1000mL, boil 30 minutes, filter off potato ball, filtrate is complemented to 800,1000mL with deionized water.
- 2. prepare the method for composite flora as claimed in claim 1, it is characterised in that：1) actication of culture：Using the general branch acid bacillus of 2-10 parts, 10-20 part wiltings bacillus pumilis, 20-30 parts white-rot fungi, 10-15 parts pseudomonas putida, 20-30 parts agrobacterium, 10-30 parts Pseudomonas stutzeri, 20-30 part enterobacter cloacaes, will The strain is inoculated on the slant medium in test tube, is cultivated 3-7 days respectively at 25-30 DEG C；2) Shaking culture：Bacteria suspension is made with sterilized water in above-mentioned slant culture, then by 10-15 parts pseudomonas putida, 20- 30 parts of agrobacteriums, 10-30 parts Pseudomonas stutzeri, 20-30 part enterobacter cloacaes, which are inoculated into, produces biosurfactant training Support and cultivated respectively in base, cultivated 3~7 days at 25~30 DEG C；The general branch acid bacillus of 2-10 parts, the short and small bar of 10-20 parts wilting Bacterium, 20-30 part white-rot fungis are inoculated into bacteria culture media and cultivated respectively, are cultivated 3~7 days at 25~30 DEG C.
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| CN201710735660.8A CN107475150A (en) | 2017-08-24 | 2017-08-24 | Compound flora for decolorizing papermaking industrial wastewater and preparation method thereof |
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Cited By (3)
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| CN110713950A (en) * | 2019-11-07 | 2020-01-21 | 云南嘉谷环保有限公司 | Organic wastewater composite microbial treatment agent and use method thereof |
| CN111302562A (en) * | 2019-12-05 | 2020-06-19 | 柏威达(南京)环境科技有限公司 | Method for treating industrial wastewater generated by papermaking |
| CN112522148A (en) * | 2020-12-09 | 2021-03-19 | 江南大学 | Method for culturing high-performance composite flora by using printing and dyeing wastewater |
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| CN1990854A (en) * | 2005-12-27 | 2007-07-04 | 中国科学院沈阳应用生态研究所 | Microorganism bacterium agent for processing thick oil sewage and preparation method thereof |
| CN103642712A (en) * | 2013-11-08 | 2014-03-19 | 华南理工大学 | Composite flora for degrading papermaking waste water and preparation method thereof |
| CN104830741A (en) * | 2015-05-28 | 2015-08-12 | 陕西科技大学 | Preparing method of compound microorganism agent for papermaking wastewater |
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2017
- 2017-08-24 CN CN201710735660.8A patent/CN107475150A/en not_active Withdrawn
Patent Citations (3)
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| CN1990854A (en) * | 2005-12-27 | 2007-07-04 | 中国科学院沈阳应用生态研究所 | Microorganism bacterium agent for processing thick oil sewage and preparation method thereof |
| CN103642712A (en) * | 2013-11-08 | 2014-03-19 | 华南理工大学 | Composite flora for degrading papermaking waste water and preparation method thereof |
| CN104830741A (en) * | 2015-05-28 | 2015-08-12 | 陕西科技大学 | Preparing method of compound microorganism agent for papermaking wastewater |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110713950A (en) * | 2019-11-07 | 2020-01-21 | 云南嘉谷环保有限公司 | Organic wastewater composite microbial treatment agent and use method thereof |
| CN111302562A (en) * | 2019-12-05 | 2020-06-19 | 柏威达(南京)环境科技有限公司 | Method for treating industrial wastewater generated by papermaking |
| CN112522148A (en) * | 2020-12-09 | 2021-03-19 | 江南大学 | Method for culturing high-performance composite flora by using printing and dyeing wastewater |
| CN112522148B (en) * | 2020-12-09 | 2022-10-14 | 江南大学 | Method for culturing high-performance composite flora by using printing and dyeing wastewater |
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