CN104830741A - Preparing method of compound microorganism agent for papermaking wastewater - Google Patents

Preparing method of compound microorganism agent for papermaking wastewater Download PDF

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CN104830741A
CN104830741A CN201510283621.XA CN201510283621A CN104830741A CN 104830741 A CN104830741 A CN 104830741A CN 201510283621 A CN201510283621 A CN 201510283621A CN 104830741 A CN104830741 A CN 104830741A
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liquid nutrient
nutrient medium
micro organism
organism fungicide
complex micro
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龚国利
张甜
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Shaanxi University of Science and Technology
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C02F3/347—Use of yeasts or fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
    • C02F2103/26—Nature of the water, waste water, sewage or sludge to be treated from the processing of plants or parts thereof
    • C02F2103/28—Nature of the water, waste water, sewage or sludge to be treated from the processing of plants or parts thereof from the paper or cellulose industry

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Abstract

The invention provides a preparing method of a compound microorganism agent for papermaking wastewater. The compound microorganism agent is prepared by mixing Trametes versicolor, Phanerochaete chrysosporium, Bacillus subtilis, Bacillus pumilus, Pseudomonas alcaligenes and cellulase; the cellulase can decompose celluloses in the wastewater into glucose; compound microorganism can also degrade solids, lignin and the like in the wastewater. The compound microorganism agent is free of chemicals, environment friendly, non-toxic and free of secondary pollution, is simple to use, is insusceptible to environmental factors, has stable effect on wastewater treatment and allows the quality of effluent to meet the national standard.

Description

A kind of preparation method of paper waste complex micro organism fungicide
Technical field
The present invention relates to technical field of sewage, particularly relate to a kind of preparation of paper waste complex micro organism fungicide.
Background technology
In paper industry, waste water is the difficult organic waste water that a kind of water yield is large, suspension content is large, colourity is high, organic concentration is high, and the raw material of use, based on straw, timber, reed, rag, is isolated Mierocrystalline cellulose through High Temperature High Pressure boiling, made paper pulp.
Paper waste is divided into again the plain boiled water generated in slurry paper waste, pulp washing bleaching stage casing paper waste and copy paper operation, and paper pulp papermaking waste water processed is commonly called as black liquor, containing a large amount of Mierocrystalline cellulose, pigment, xylogen and inorganic salt etc. in black liquor, a large amount of Mierocrystalline cellulose and filler etc. are then had in middle section water and plain boiled water, in paper waste, black liquor is that harm is maximum, its pollutent accounts for more than 90% of industrial pollution caused by paper manufacturing total emission volumn, because black liquor color is dark, alkalescence is large, foam is many, stink weight, and a large amount of dissolved oxygen consumed in water, severe contamination water source, all harm is brought to human health and environment, current employing tradition multi-medium filtering, sand filtration, activated carbon filtration technique is to waste water recycling process, also concentration of suspension in water can only be reduced to a certain extent, to the ammonia nitrogen in waste water, soluble pollutants, COD and salt grade and cannot remove further, traditional activated sludge process utilizes active sludge to process waste water exactly, it can be subject to the factor impacts such as environment and apply restricted.
Summary of the invention
The present invention, according to the distinctive pollutent of paper waste, provides a kind of complex micro organism fungicide, can effectively reduce solid content in waste water, improve water body color, make effluent quality reach discharging standards.
In order to achieve the above object, the technical solution used in the present invention comprises the following steps:
Step 1, activation and each bacterial classification of enlarged culturing:
First rainbow conk keyhole bacterium, the flat lead fungi of yellow archespore, subtilis, bacillus pumilus and Pseudomonas alcaligenes are activated respectively on solid medium flat board, then the single bacterium colony on each solid medium after activation is carried out liquid nutrient medium enlarged culturing respectively, make thalline content reach 1.0 × 10 8-1.0 × 10 9individual/mL;
Step 2, centrifugation, drying obtain each microbial germ powder:
Each microbial inoculum step 1 enlarged culturing obtained is through centrifugation, and collecting precipitation is microbial cells, obtains each microbial germ powder after drying;
Step 3, prepare complex micro organism fungicide:
Bacterium powder and the cellulase of rainbow conk keyhole bacterium step 2 obtained, the flat lead fungi of yellow archespore, subtilis, bacillus pumilus and Pseudomonas alcaligenes are mixed and made into complex micro organism fungicide, in mass fraction, wherein rainbow conk keyhole bacterium 15-30 part, yellow archespore flat lead fungi 10-20 part, subtilis 10-20 part, bacillus pumilus 3-10 part, Pseudomonas alcaligenes 5-20 part, cellulase 1-3 part.
The liquid nutrient medium of described rainbow conk keyhole bacterium and the flat lead fungi of yellow archespore is KH 2pO 41.0g, Na 2hPO 40.2g, MgSO 47H 2o 0.5g, VB10.1mg, CaCl 20.1mg, FeSO 47H 2o 0.1mg, ZnSO 47H 2o 0.01mg, CuSO 45H 2o 0.2mg, glucose 1.0g, ammonium tartrate 0.1g and distilled water 1L mix, and adjust ph is 5-6;
The liquid nutrient medium of described subtilis and bacillus pumilus is glucose 20g, and peptone 15g, extractum carnis 0.5g, NaCl 5g and distilled water 1L mix, and adjust ph is 7.0;
The liquid nutrient medium of described Pseudomonas alcaligenes is Tryptones 10g, K 2hPO 41.5g, MgSO 47H 2o 1.5g, glycerine 10mL and distilled water 1L mix, and adjust ph is 7.2;
The solid medium of each bacterial classification is made for adding 15g agar powder in its liquid nutrient medium above, and liquid nutrient medium and solid medium all will through 121 DEG C of sterilizing 30min.
Described cellulase activity is 1-10 ten thousand U/g.
Described liquid nutrient medium enlarged culturing is 28-30 DEG C of cultivation.
Described liquid nutrient medium enlarged culturing comprises triangular flask one-level enlarged culturing and fermentor tank secondary enlarged culturing.
Described liquid nutrient medium enlarged culturing specifically comprises the following steps, first the liquid nutrient medium of each bacterial classification is added in triangular flask, then the single bacterium colony on each solid medium is accessed in corresponding triangular flask respectively, be cultured to thalline content in each triangular flask and reach 1.0 × 10 8-1.0 × 10 9individual/mL; Then the liquid nutrient medium of each bacterial classification is added in fermentor tank, and the thalline in triangular flask is accessed in corresponding fermentor tank, be cultured to thalline content in each fermentor tank and reach 1.0 × 10 8-1.0 × 10 9individual/mL.
The drying temperature of described step 2 is 30-40 DEG C.
The complex microorganism mentioned in the present invention is rainbow conk keyhole bacterium, the flat lead fungi of yellow archespore, subtilis, bacillus pumilus and Pseudomonas alcaligenes, is specially:
Rainbow conk keyhole bacterium (Trametes versicolor) CGMCC No.5987;
Yellow archespore flat lead fungi (Phanerochaete chrysosporium) CGMCC No.5776;
Subtilis (Bacillus subtillus) CGMCC No.17408;
Bacillus pumilus (Bacillus pumilus) CGMCC No.1326
Pseudomonas alcaligenes (Pseudomonas alacaligenes) CGMCC No.11805.
The above-mentioned bacterial classification that the present invention mentions all can be bought from China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) and obtain.
Compared with prior art, the present invention at least has following beneficial effect, the feature that the present invention is directed to paper waste devises a kind of complex micro organism fungicide, each bacterial classification of independent expansion, have employed enlarged culturing step by step, the production optimization of each bacterial classification can be reached, on this basis microorganism is combined with cellulase, cellulose decomposition unnecessary in waste water is made to be the carbon source that glucose can be used as microorganism, microorganism is degraded to the xylogen in waste water, waste water quality is purified, reaches discharging standards; And the good and non-secondary pollution of outlet effect of the present invention, therefore in pulping wastewater treatment, prospect of the present invention is considerable.
Embodiment
Below in conjunction with specific embodiment, the present invention is described further, described in be explanation of the invention instead of restriction, any enlarged culturing makes cell concentration meet the requirements of training method.
The complex microorganism mentioned in the present invention is rainbow conk keyhole bacterium, the flat lead fungi of yellow archespore, subtilis, bacillus pumilus and Pseudomonas alcaligenes, is specially:
Rainbow conk keyhole bacterium (Trametes versicolor) CGMCC No.5987;
Yellow archespore flat lead fungi (Phanerochaete chrysosporium) CGMCC No.5776;
Subtilis (Bacillus subtillus) CGMCC No.17408;
Bacillus pumilus (Bacillus pumilus) CGMCC No.1326
Pseudomonas alcaligenes (Pseudomonas alacaligenes) CGMCC No.11805.
The above-mentioned bacterial classification that the present invention mentions all can be bought from China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) and obtain.
The liquid nutrient medium of each bacterial classification that the embodiment of the present invention relates to:
The liquid nutrient medium of rainbow conk keyhole bacterium and the flat lead fungi of yellow archespore is KH 2pO 41.0g, Na 2hPO 40.2g, MgSO 47H 2o0.5g, VB10.1mg, CaCl 20.1mg, FeSO 47H 2o 0.1mg, ZnSO 47H 2o 0.01mg, CuSO 45H 2o 0.2mg, glucose 1.0g, ammonium tartrate 0.1g and distilled water 1L mix, and adjust ph is 5-6;
The liquid nutrient medium of subtilis and bacillus pumilus is glucose 20g, and peptone 15g, extractum carnis 0.5g, NaCl 5g and distilled water 1L mix, and adjust ph is 7.0;
The liquid nutrient medium of Pseudomonas alcaligenes is Tryptones 10g, K 2hPO 41.5g, MgSO 47H 2o 1.5g, glycerine 10mL and distilled water 1L mix, and adjust ph is 7.2;
The solid medium of each bacterial classification that the embodiment of the present invention relates to:
The solid medium of each bacterial classification is made for adding 15g agar powder in its liquid nutrient medium above, and liquid nutrient medium and solid medium all will through 121 DEG C of sterilizing 30min.
Embodiment 1
Step 1, activation and each bacterial classification of enlarged culturing:
First rainbow conk keyhole bacterium, the flat lead fungi of yellow archespore, subtilis, bacillus pumilus and Pseudomonas alcaligenes are activated respectively on solid medium flat board; Then, the liquid nutrient medium of each bacterial classification is added in different triangular flasks, and the single bacterium colony on each solid medium is accessed in corresponding triangular flask respectively, at 28-30 DEG C, be cultured to thalline content in each triangular flask reach 1.0 × 10 8-1.0 × 10 9individual/mL; Then the liquid nutrient medium of each bacterial classification is added in different fermentor tanks, and the thalline in triangular flask is accessed in corresponding fermentor tank, at 28-30 DEG C, be cultured to thalline content in each fermentor tank reach 1.0 × 10 8-1.0 × 10 9individual/mL;
Step 2, centrifugation, drying obtain each microbial germ powder:
Step 1 fermentor tank is expanded each microbial inoculum of obtaining through centrifugation, collecting precipitation is microbial cells, and 30 DEG C of dryings obtain each microbial germ powder;
Step 3, prepare complex micro organism fungicide:
The rainbow conk keyhole bacterium of step 2, the flat lead fungi of yellow archespore, subtilis, bacillus pumilus and Pseudomonas alcaligenes bacterium powder and cellulase are mixed and made into complex micro organism fungicide, in mass fraction, wherein rainbow conk keyhole bacterium 20 parts, the flat lead fungi of yellow archespore 15 parts, subtilis 10 parts, bacillus pumilus 3 parts, Pseudomonas alcaligenes 5 parts, cellulase 3 parts, wherein cellulase activity is 1-10 ten thousand U/g.
Embodiment 2
Step 1, activation and each bacterial classification of enlarged culturing:
First rainbow conk keyhole bacterium, the flat lead fungi of yellow archespore, subtilis, bacillus pumilus and Pseudomonas alcaligenes are activated respectively on solid medium flat board; Then, the liquid nutrient medium of each bacterial classification is added in different triangular flasks, and the single bacterium colony on each solid medium is accessed in corresponding triangular flask respectively, at 28-30 DEG C, be cultured to thalline content in each triangular flask reach 1.0 × 10 8-1.0 × 10 9individual/mL; Then the liquid nutrient medium of each bacterial classification is added in different fermentor tanks, and the thalline in triangular flask is accessed in corresponding fermentor tank, at 28-30 DEG C, be cultured to thalline content in each fermentor tank reach 1.0 × 10 8-1.0 × 10 9individual/mL;
Step 2, centrifugation, drying obtain each microbial germ powder:
Step 1 fermentor tank is expanded each microbial inoculum of obtaining through centrifugation, collecting precipitation is microbial cells, and 35 DEG C of dryings obtain each microbial germ powder.
Step 3, prepare complex micro organism fungicide:
The rainbow conk keyhole bacterium of step 2, the flat lead fungi of yellow archespore, subtilis, bacillus pumilus and Pseudomonas alcaligenes bacterium powder and cellulase are mixed and made into complex micro organism fungicide, by quality ratio, wherein rainbow conk keyhole bacterium 25 parts, the flat lead fungi of yellow archespore 10 parts, subtilis 15 parts, bacillus pumilus 5 parts, Pseudomonas alcaligenes 15 parts, cellulase 1 part, wherein cellulase activity is 1-10 ten thousand U/g.
Embodiment 3
Step 1, activation and each bacterial classification of enlarged culturing:
First rainbow conk keyhole bacterium, the flat lead fungi of yellow archespore, subtilis, bacillus pumilus and Pseudomonas alcaligenes are activated respectively on solid medium flat board; Then, the liquid nutrient medium of each bacterial classification is added in different triangular flasks, and the single bacterium colony on each solid medium is accessed in corresponding triangular flask respectively, at 28-30 DEG C, be cultured to thalline content in each triangular flask reach 1.0 × 10 8-1.0 × 10 9individual/mL; Then the liquid nutrient medium of each bacterial classification is added in different fermentor tanks, and the thalline in triangular flask is accessed in corresponding fermentor tank, at 28-30 DEG C, be cultured to thalline content in each fermentor tank reach 1.0 × 10 8-1.0 × 10 9individual/mL;
Step 2, centrifugation, drying obtain each microbial germ powder:
Step 1 fermentor tank is expanded each microbial inoculum of obtaining through centrifugation, collecting precipitation is microbial cells, and 35 DEG C of dryings obtain each microbial germ powder.
Step 3, prepare complex micro organism fungicide:
The rainbow conk keyhole bacterium of step 2, the flat lead fungi of yellow archespore, subtilis, bacillus pumilus and Pseudomonas alcaligenes bacterium powder and cellulase are mixed and made into complex micro organism fungicide, by quality ratio, wherein rainbow conk keyhole bacterium 15 parts, the flat lead fungi of yellow archespore 13 parts, subtilis 20 parts, bacillus pumilus 9 parts, Pseudomonas alcaligenes 10 parts, cellulase 2 parts, wherein cellulase activity is 1-10 ten thousand U/g.
Embodiment 4
Step 1, activation and each bacterial classification of enlarged culturing:
First rainbow conk keyhole bacterium, the flat lead fungi of yellow archespore, subtilis, bacillus pumilus and Pseudomonas alcaligenes are activated respectively on solid medium flat board; Then, the liquid nutrient medium of each bacterial classification is added in different triangular flasks, and the single bacterium colony on each solid medium is accessed in corresponding triangular flask respectively, at 28-30 DEG C, be cultured to thalline content in each triangular flask reach 1.0 × 10 8-1.0 × 10 9individual/mL; Then the liquid nutrient medium of each bacterial classification is added in different fermentor tanks, and the thalline in triangular flask is accessed in corresponding fermentor tank, at 28-30 DEG C, be cultured to thalline content in each fermentor tank reach 1.0 × 10 8-1.0 × 10 9individual/mL;
Step 2, centrifugation, drying obtain each microbial germ powder:
Step 1 fermentor tank is expanded each microbial inoculum of obtaining through centrifugation, collecting precipitation is microbial cells, and 40 DEG C of dryings obtain each microbial germ powder.
Step 3, prepare complex micro organism fungicide:
The rainbow conk keyhole bacterium of step 2, the flat lead fungi of yellow archespore, subtilis, bacillus pumilus and Pseudomonas alcaligenes bacterium powder and cellulase are mixed and made into complex micro organism fungicide, by quality ratio, wherein rainbow conk keyhole bacterium 20 parts, the flat lead fungi of yellow archespore 20 parts, subtilis 15 parts, bacillus pumilus 10 parts, Pseudomonas alcaligenes 8 parts, cellulase 3 parts, wherein cellulase activity is 1-10 ten thousand U/g.
Embodiment 5
Step 1, activation and each bacterial classification of enlarged culturing:
First rainbow conk keyhole bacterium, the flat lead fungi of yellow archespore, subtilis, bacillus pumilus and Pseudomonas alcaligenes are activated respectively on solid medium flat board; Then, the liquid nutrient medium of each bacterial classification is added in different triangular flasks, and the single bacterium colony on each solid medium is accessed in corresponding triangular flask respectively, at 28-30 DEG C, be cultured to thalline content in each triangular flask reach 1.0 × 10 8-1.0 × 10 9individual/mL; Then the liquid nutrient medium of each bacterial classification is added in different fermentor tanks, and the thalline in triangular flask is accessed in corresponding fermentor tank, at 28-30 DEG C, be cultured to thalline content in each fermentor tank reach 1.0 × 10 8-1.0 × 10 9individual/mL;
Step 2, centrifugation, drying obtain each microbial germ powder:
Step 1 fermentor tank is expanded each microbial inoculum of obtaining through centrifugation, collecting precipitation is microbial cells, and 38 DEG C of dryings obtain each microbial germ powder.
Step 3, prepare complex micro organism fungicide:
The rainbow conk keyhole bacterium of step 2, the flat lead fungi of yellow archespore, subtilis, bacillus pumilus and Pseudomonas alcaligenes bacterium powder and cellulase are mixed and made into complex micro organism fungicide, by quality ratio, wherein rainbow conk keyhole bacterium 30 parts, the flat lead fungi of yellow archespore 16 parts, subtilis 18 parts, bacillus pumilus 8 parts, Pseudomonas alcaligenes 20 parts, cellulase 2 parts, wherein cellulase activity is 1-10 ten thousand U/g.
Embodiment 6
Step 1, activation and each bacterial classification of enlarged culturing:
First rainbow conk keyhole bacterium, the flat lead fungi of yellow archespore, subtilis, bacillus pumilus and Pseudomonas alcaligenes are activated respectively on solid medium flat board; Then, the liquid nutrient medium of each bacterial classification is added in different triangular flasks, and the single bacterium colony on each solid medium is accessed in corresponding triangular flask respectively, at 28-30 DEG C, be cultured to thalline content in each triangular flask reach 1.0 × 10 8-1.0 × 10 9individual/mL; Then the liquid nutrient medium of each bacterial classification is added in different fermentor tanks, and the thalline in triangular flask is accessed in corresponding fermentor tank, at 28-30 DEG C, be cultured to thalline content in each fermentor tank reach 1.0 × 10 8-1.0 × 10 9individual/mL;
Step 2, centrifugation, drying obtain each microbial germ powder:
Step 1 fermentor tank is expanded each microbial inoculum of obtaining through centrifugation, collecting precipitation is microbial cells, and 30 DEG C of dryings obtain each microbial germ powder.
Step 3, prepare complex micro organism fungicide:
The rainbow conk keyhole bacterium of step 2, the flat lead fungi of yellow archespore, subtilis, bacillus pumilus and Pseudomonas alcaligenes bacterium powder and cellulase are mixed and made into complex micro organism fungicide, by quality ratio, wherein rainbow conk keyhole bacterium 28 parts, the flat lead fungi of yellow archespore 18 parts, subtilis 15 parts, bacillus pumilus 10 parts, Pseudomonas alcaligenes 13 parts, cellulase 1 part, wherein cellulase activity is 1-10 ten thousand U/g.
Present invention employs enlarged culturing step by step, the production optimization of each bacterial classification can be reached, on this basis microorganism is combined with cellulase, cellulose decomposition unnecessary in waste water is made to be the carbon source that glucose can be used as microorganism, microorganism is degraded to the xylogen in waste water, waste water quality is purified, reaches discharging standards; And the good and non-secondary pollution of outlet effect of the present invention, therefore in pulping wastewater treatment, prospect is considerable.

Claims (7)

1. a preparation method for paper waste complex micro organism fungicide, is characterized in that, comprises the following steps:
Step 1, activation and each bacterial classification of enlarged culturing:
First rainbow conk keyhole bacterium, the flat lead fungi of yellow archespore, subtilis, bacillus pumilus and Pseudomonas alcaligenes are activated respectively on solid medium flat board, then the single bacterium colony on each solid medium after activation is carried out liquid nutrient medium enlarged culturing respectively, make thalline content reach 1.0 × 10 8-1.0 × 10 9individual/mL;
Step 2, centrifugation, drying obtain each microbial germ powder:
Each microbial inoculum step 1 enlarged culturing obtained is through centrifugation, and collecting precipitation is microbial cells, obtains each microbial germ powder after drying;
Step 3, prepare complex micro organism fungicide:
Bacterium powder and the cellulase of rainbow conk keyhole bacterium step 2 obtained, the flat lead fungi of yellow archespore, subtilis, bacillus pumilus and Pseudomonas alcaligenes are mixed and made into complex micro organism fungicide, in mass fraction, wherein rainbow conk keyhole bacterium 15-30 part, yellow archespore flat lead fungi 10-20 part, subtilis 10-20 part, bacillus pumilus 3-10 part, Pseudomonas alcaligenes 5-20 part, cellulase 1-3 part.
2. the preparation method of a kind of paper waste complex micro organism fungicide according to claim 1, is characterized in that: the liquid nutrient medium of described rainbow conk keyhole bacterium and the flat lead fungi of yellow archespore is KH 2pO 41.0g, Na 2hPO 40.2g, MgSO 47H 2o 0.5g, VB1 0.1mg, CaCl 20.1mg, FeSO 47H 2o 0.1mg, ZnSO 47H 2o 0.01mg, CuSO 45H 2o 0.2mg, glucose 1.0g, ammonium tartrate 0.1g and distilled water 1L mix, and adjust ph is 5-6;
The liquid nutrient medium of described subtilis and bacillus pumilus is glucose 20g, and peptone 15g, extractum carnis 0.5g, NaCl 5g and distilled water 1L mix, and adjust ph is 7.0;
The liquid nutrient medium of described Pseudomonas alcaligenes is Tryptones 10g, K 2hPO 41.5g, MgSO 47H 2o 1.5g, glycerine 10mL and distilled water 1L mix, and adjust ph is 7.2;
The solid medium of each bacterial classification is made for adding 15g agar powder in its liquid nutrient medium above, and liquid nutrient medium and solid medium all will through 121 DEG C of sterilizing 30min.
3. the preparation method of a kind of paper waste complex micro organism fungicide according to claim 1, is characterized in that: described cellulase activity is 1-10 ten thousand U/g.
4. the preparation method of a kind of paper waste complex micro organism fungicide according to claim 1, is characterized in that: described liquid nutrient medium enlarged culturing is 28-30 DEG C of cultivation.
5. the preparation method of a kind of paper waste complex micro organism fungicide according to claim 1, is characterized in that: described liquid nutrient medium enlarged culturing comprises triangular flask one-level enlarged culturing and fermentor tank secondary enlarged culturing.
6. the preparation method of a kind of paper waste complex micro organism fungicide according to claim 5, it is characterized in that: described liquid nutrient medium enlarged culturing specifically comprises the following steps, first the liquid nutrient medium of each bacterial classification is added in triangular flask, then the single bacterium colony on each solid medium is accessed in corresponding triangular flask respectively, be cultured to thalline content in each triangular flask and reach 1.0 × 10 8-1.0 × 10 9individual/mL; Then the liquid nutrient medium of each bacterial classification is added in fermentor tank, and the thalline in triangular flask is accessed in corresponding fermentor tank, be cultured to thalline content in each fermentor tank and reach 1.0 × 10 8-1.0 × 10 9individual/mL.
7. the preparation method of a kind of paper waste complex micro organism fungicide according to claim 1, is characterized in that: the drying temperature of described step 2 is 30-40 DEG C.
CN201510283621.XA 2015-05-28 2015-05-28 Preparing method of compound microorganism agent for papermaking wastewater Pending CN104830741A (en)

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Cited By (5)

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CN106497846A (en) * 2016-12-14 2017-03-15 钦州学院 A kind of wastewater treatment microbial microbial inoculum and preparation method thereof
CN106676021A (en) * 2015-11-06 2017-05-17 丹阳市尚德生物科技有限公司 Microbial preparation for river course water quality restoration, and preparation method thereof
CN106731737A (en) * 2015-11-23 2017-05-31 江阴昊松格氏生物技术有限公司 Complex microorganism deodorant with Radix Codonopsis peppermint lemon nostoc
CN107475150A (en) * 2017-08-24 2017-12-15 青岛理工大学 Composite flora that a kind of paper-making industrial waste water decolourizes and preparation method thereof
CN110964651A (en) * 2018-09-29 2020-04-07 台山市翔隆纸业有限公司 Preparation method of microbial agent for papermaking wastewater

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