CN103642702A - Fungi strain LP-16 and use thereof for treatment of zinc-containing wastewater - Google Patents
Fungi strain LP-16 and use thereof for treatment of zinc-containing wastewater Download PDFInfo
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- CN103642702A CN103642702A CN201310655461.8A CN201310655461A CN103642702A CN 103642702 A CN103642702 A CN 103642702A CN 201310655461 A CN201310655461 A CN 201310655461A CN 103642702 A CN103642702 A CN 103642702A
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- 239000011701 zinc Substances 0.000 title claims abstract description 44
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 title claims abstract description 26
- 239000002351 wastewater Substances 0.000 title claims abstract description 26
- 229910052725 zinc Inorganic materials 0.000 title claims abstract description 26
- 241000233866 Fungi Species 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 21
- 238000002955 isolation Methods 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 12
- 241000223221 Fusarium oxysporum Species 0.000 claims abstract description 7
- 238000002360 preparation method Methods 0.000 claims abstract description 6
- 230000001580 bacterial effect Effects 0.000 claims description 41
- 230000002538 fungal effect Effects 0.000 claims description 32
- 150000002500 ions Chemical class 0.000 claims description 30
- 239000007788 liquid Substances 0.000 claims description 26
- 235000015097 nutrients Nutrition 0.000 claims description 21
- 239000002054 inoculum Substances 0.000 claims description 17
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 claims description 13
- 229940049547 paraxin Drugs 0.000 claims description 13
- 229920001817 Agar Polymers 0.000 claims description 11
- 239000008272 agar Substances 0.000 claims description 11
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- 244000061456 Solanum tuberosum Species 0.000 claims description 8
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 8
- 239000008103 glucose Substances 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 239000011573 trace mineral Substances 0.000 claims description 8
- 235000013619 trace mineral Nutrition 0.000 claims description 8
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 7
- 229940041514 candida albicans extract Drugs 0.000 claims description 7
- 238000000855 fermentation Methods 0.000 claims description 7
- 230000004151 fermentation Effects 0.000 claims description 7
- 239000002068 microbial inoculum Substances 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 7
- 239000012138 yeast extract Substances 0.000 claims description 7
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 6
- 230000009514 concussion Effects 0.000 claims description 6
- 235000017281 sodium acetate Nutrition 0.000 claims description 6
- 239000001632 sodium acetate Substances 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 5
- 244000005700 microbiome Species 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 239000011734 sodium Substances 0.000 claims description 4
- 238000005273 aeration Methods 0.000 claims description 3
- 238000001179 sorption measurement Methods 0.000 abstract description 11
- PTFCDOFLOPIGGS-UHFFFAOYSA-N Zinc dication Chemical compound [Zn+2] PTFCDOFLOPIGGS-UHFFFAOYSA-N 0.000 abstract description 8
- 230000000813 microbial effect Effects 0.000 abstract description 3
- 239000013043 chemical agent Substances 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract description 2
- 238000004064 recycling Methods 0.000 abstract 2
- 239000002244 precipitate Substances 0.000 abstract 1
- 239000010802 sludge Substances 0.000 abstract 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 18
- 238000010521 absorption reaction Methods 0.000 description 16
- 229910001385 heavy metal Inorganic materials 0.000 description 10
- 238000006243 chemical reaction Methods 0.000 description 9
- 230000000694 effects Effects 0.000 description 9
- 241001052560 Thallis Species 0.000 description 5
- 238000012258 culturing Methods 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 230000010355 oscillation Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 238000011109 contamination Methods 0.000 description 2
- 230000001186 cumulative effect Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000011084 recovery Methods 0.000 description 2
- 241001417490 Sillaginidae Species 0.000 description 1
- 230000000274 adsorptive effect Effects 0.000 description 1
- 229940040526 anhydrous sodium acetate Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010170 biological method Methods 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000009713 electroplating Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000005272 metallurgy Methods 0.000 description 1
- 230000007483 microbial process Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000004065 wastewater treatment Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
- Y02P10/20—Recycling
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a fungi strain LP-16 (fusarium oxysporum) capable of removing or recycling zinc ions in water, an isolation medium for the fungi strain LP-16, a microbial agent containing the strain, a preparation method for the microbial agent, a zinc ion treatment agent containing the strain and a method for treating zinc-containing wastewater. The fungi strain LP-16 has the advantages of high growth speed, large hypha surface area, high adsorbance and high adsorption capacity, and can be used for effectively removing or recycling the zinc ions in the zinc-containing wastewater. In the method for treating the zinc-containing wastewater, the addition of a great amount of chemical agent is avoided, so that the subsequent production of a great amount of waste such as precipitates and sludge is avoided, and the method has the advantages of low investment and high yield.
Description
Technical field
The present invention relates to microbial technology field, relate in particular to a kind of fungal bacterial strain LP-16 and the purposes in zinc-containing water is processed thereof that can remove zine ion in water.
Background technology
The waste water that contains heavy metal has great harm to the mankind and environment, and along with national environmental protection consciousness improves constantly, a series of relevant law and standard are progressively set up and be perfect.Especially after country " 12 ", for the improvement of heavy metal, more pay attention to.In the industries such as metallurgy, plating, chemical industry, electronics, mechanical workout, all can produce a large amount of waste water that contains heavy metal, traditional method can not meet the needs of industry development, and waste water how efficient, that the processing of low consumption contains heavy metal becomes a key and significant problem.
Domestic zinc-containing water is mainly derived from zinc and smelts and electroplating enterprise.Because relevant enterprise is founded the factory early, machinery equipment and treatment process are outmoded, and for the wastewater treatment that contains heavy metal, also often in falling behind level, the rate of recovery of waste water is low, the water quality inferiority of recovery.After the outer row of waste water, environmental pollution is serious, and relevant heavy metal contaminants also cannot reclaim and utilize, and resource obtains serious waste.
In recent years, biological method especially method of microorganism progressively by people, be familiar with and accept administering heavy metal contamination field.That microbial process has is with low cost, operation operation is easy, processing efficiency is high, non-secondary pollution, be easy to reclaim the advantages such as heavy metal.At present, microbiological treatment technology has become a large focus of countries in the world Heavy Metal Pollution Control research and Application Areas.In this area, exist for processing respond well containing zinc sewage and can carrying out the needs of the bacterial classification of large-scale industrial application.
Summary of the invention
First object of the present invention is to provide a kind of fungal bacterial strain LP-16 (Fusarium oxysporum).
Second object of the present invention has been to provide the isolation medium of above-mentioned fungal bacterial strain.
The 3rd object of the present invention is to provide a kind of microbial inoculum that comprises above-mentioned fungal bacterial strain.
The 4th object of the present invention is to provide the preparation method of above-mentioned microbial inoculum.
The 5th object of the present invention is to provide a kind of zine ion treatment agent.
The 6th object of the present invention is to provide a kind of method of Treatment of Zinc-containing Wastewater.
For reaching this object, the present invention by the following technical solutions:
First aspect, the invention provides a kind of fungal bacterial strain LP-16 (Fusarium oxysporum), be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, 100101, preservation date is on August 16th, 2013, and deposit number is CGMCC No.8037.
Second aspect, the invention provides a kind of as described in first aspect the isolation medium of fungal bacterial strain LP-16, for separating of described fungal bacterial strain LP-16; Described isolation medium comprises 0.1～0.4g/L sodium acetate, 0.05～0.2g/L yeast extract, 5～25g/L agar, 40～60mg/L Zn
2+, 0.01～0.12g/L MgSO
47H
2o, 0.001～0.010g/L K
2hPO
4with 0.05～0.2g/L paraxin, and pH=7.0;
Preferably, described isolation medium comprises 0.2～0.3g/L sodium acetate, 0.1～0.2g/L yeast extract, 10～20g/L agar, 45～55mg/L Zn
2+, 0.03～0.1g/L MgSO
47H
2o, 0.003～0.008g/L K
2hPO
4with 0.08～0.15g/L paraxin, and pH=7.0;
Further preferably, described isolation medium comprises 0.2461g/L sodium acetate, 0.15g/L yeast extract, 15g/L agar, 50mg/L Zn
2+, 0.05g/L MgSO
47H
2o, 0.005g/L K
2hPO
4with 0.1g/L paraxin, and pH=7.0.
Preferably, also add 2ml trace element solution in isolation medium every liter described, described trace element solution comprises 1～5g/L CaCl
22H
2o, 1～5g/L2.5g H
3bO
3, 0.5～1.2g/L ZnCl
24H
2o, 0.5～1.5g/L FeCl
36H
2o, 0.3～0.6g/L ZnSO
47H
2o, 0.1～0.4g/L Na
2moO
42H
2o and 0.003～0.008g/LCuSO
45H
2o.
Further preferably, described trace element solution comprises 2～4g/L CaCl
22H
2o, 1～3g/L H
3bO
3, 0.7～1g/LZnCl
24H
2o, 0.8～1.2g/L FeCl
36H
2o, 0.4～0.5g/L ZnSO
47H
2o, 0.2～0.3g/LNa
2moO
42H
2o and 0.005～0.006g/L CuSO
45H
2o.
Still more preferably, described trace element solution comprises 3.7g CaCl
22H
2o, 2.5g H
3bO
3, 0.87gZnCl
24H
2o, 1.0g FeCl
36H
2o, 0.44g ZnSO
47H
2o, 0.29g Na
2moO
42H
2o and 0.005g CuSO
45H
2o.
The third aspect, the invention provides a kind of fungal inoculant, and this fungal inoculant comprises the fungal bacterial strain LP-16 described in first aspect.
Fourth aspect, the invention provides the preparation method of the fungal inoculant as described in the third aspect, and described method comprises:
(1) culture dish is cultivated: fungal bacterial strain LP-16 as claimed in claim 1 is inoculated on solid medium under aseptic condition to 20-30 ℃, preferably 22-28 ℃, further preferably cultivate at 25 ℃ 4-6 days, preferably 4.4-5.5 days, further preferably 5 days; Described solid medium comprises following component: potato is soaked powder 4-6g/L, preferred 4.5-5.5g/L, further preferred 5g/L, glucose or sucrose 15-25g/L, preferred 17-23g/L, further preferred 20g/L, agar 13-18g/L, preferred 14-17g/L, further preferred 16g/L, paraxin 0.1g/L;
(2) first order seed is cultivated: the bacterial classification that step (1) is cultivated is inoculated in liquid nutrient medium under aseptic condition, 20-30 ℃, and preferred 22-28 ℃, further preferably cultivates 5-7 days at 25 ℃, and preferably 5.5-6.5 days, more preferably 6 days, makes first order seed; Described liquid nutrient medium comprises following component: potato is soaked powder 4-6g/L, preferred 4.5-5.5g/L, further preferred 5g/L, glucose or sucrose 15-25g/L, preferred 17-23g/L, further preferred 20g/L, paraxin 0.1g/L;
(3) secondary seed is cultivated: by the volume ratio of liquid nutrient medium, be 5-15%, preferably 8-12%, more preferably 10% inoculum size, step (2) gained first order seed is inoculated in fermentor tank, aeration, cultivates 5-7 days, preferably 5.5-6.5 days, further preferably 6 days, makes secondary seed;
(4) mixing fermentation culture: be 10-20%, preferably 12-18%, more preferably 15% inoculum size by the volume ratio of liquid nutrient medium, secondary seed is inoculated in fermentor tank, carry out high density fermentation cultivation, obtain microbial inoculum.
During culture dish is cultivated, culture temperature can be 20 ℃, 21 ℃, 22 ℃, 23 ℃, 24 ℃, 25 ℃, 26 ℃, 27 ℃, 28 ℃, 29 ℃ or 30 ℃; Incubation time can be 4 days, 4.5 days, 110 hours, 5 days, 130 hours, 5.5 days or 6 days; In solid medium used potato to soak powder can be 4g/L, 4.2g/L, 4.5g/L, 4.7g/L, 4.9g/L, 5g/L, 5.lg/L, 5.3g/L, 5.5g/L, 5.8g/L or 6g/L, glucose or sucrose can be 15g/L, 16g/L, 17g/L, 18g/L, 19g/L, 20g/L, 21g/L, 22g/L, 23g/L, 24g/L or 25g/L, and agar can be 13g/L, 14g/L, 15g/L, 16g/L, 17g/L or 18g/L.
During first order seed is cultivated, culture temperature can be 20 ℃, 21 ℃, 22 ℃, 23 ℃, 24 ℃, 25 ℃, 26 ℃, 27 ℃, 28 ℃, 29 ℃ or 30 ℃; Incubation time can be 5 days, 5.5 days, 140 hours, 6 days, 150 hours, 6.5 days or 7 days; In liquid nutrient medium used potato to soak powder can be 4g/L, 4.2g/L, 4.5g/L, 4.7g/L, 4.9g/L, 5g/L, 5.lg/L, 5.3g/L, 5.5g/L, 5.8g/L or 6g/L, glucose or sucrose can be 15g/L, 16g/L, 17g/L, 18g/L, 19g/L, 20g/L, 21g/L, 22g/L, 23g/L, 24g/L or 25g/L.
During secondary seed is cultivated, the volume ratio that inoculum size is pressed liquid nutrient medium can be 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14% or 15%; Incubation time can be 5.5 days, 140 hours, 6 days, 150 hours or 6.5 days.
In mixing fermentation culture, the volume ratio that inoculum size is pressed liquid nutrient medium can be 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19% or 20%.
The 5th aspect, the invention provides a kind of zine ion treatment agent, and described zine ion treatment agent comprises the fungal bacterial strain LP-16 as described in first aspect; Preferably, described in, be treated to and remove or reclaim.
The 6th aspect, the invention provides a kind of method of Treatment of Zinc-containing Wastewater, described method comprises: by the fungal bacterial strain LP-16 as described in first aspect, fungal inoculant as described in the third aspect or the zine ion treatment agent as described in the 5th aspect with as described in zinc-containing water contact.
Preferably, the method for Treatment of Zinc-containing Wastewater of the present invention comprises:
(1) the pH value of zinc-containing water is adjusted to 5.0-8.0, preferred 5.5-7, further preferably 6.0;
(2) in water body, add the fungal bacterial strain LP-16 as described in first aspect, fungal inoculant or the zine ion treatment agent as described in the 5th aspect as described in the third aspect, in 10-35 ℃, preferred 15-30 ℃, further preferably 25 ℃, concussion 4-8 hour, preferably 5-7 hour, further preferably 6 hours, concussion speed 90-180rpm, preferred 100-160rpm, further preferred 120rpm;
(3) standing 8-16 hour, preferred 10-14 hour, further preferably 12 hours.
In the method for Treatment of Zinc-containing Wastewater of the present invention, the pH value of zinc-containing water can be 5.0,5.5,5.8,5.9,6.0,6.1,6.2,6.5,7.0,7.5 or 8.0; Treatment temp can be 10 ℃, 11 ℃, 12 ℃, 13 ℃, 14 ℃, 15 ℃, 16 ℃, 17 ℃, 18 ℃, 19 ℃, 20 ℃, 21 ℃, 22 ℃, 23 ℃, 24 ℃, 25 ℃, 26 ℃, 27 ℃, 28 ℃, 29 ℃, 30 ℃, 31 ℃, 32 ℃, 33 ℃, 34 ℃ or 35 ℃; The concussion time can be 4 hours, 4.5 hours, 5 hours, 5.5 hours, 6 hours, 6.5 hours, 7 hours, 7.5 hours or 8 hours; Concussion speed can be 90rpm, 100rpm, 110rpm, 120rpm, 130rpm, 140rpm, 150rpm, 160rpm, 170rpm or 180rpm; Time of repose can be 8 hours, 8.5 hours, 9 hours, 9.5 hours, 10 hours, 10.5 hours, 11 hours, 11.5 hours, 12 hours, 12.5 hours, 13 hours, 13.5 hours, 14 hours, 14.5 hours, 15 hours, 15.5 hours or 16 hours.
In the method for Treatment of Zinc-containing Wastewater of the present invention, described in be treated to and remove or reclaim the zine ion in described zinc-containing water.
Beneficial effect of the present invention:
(1) fungal bacterial strain LP-16 of the present invention has growth soon, and mycelia surface-area is large, and adsorptive capacity is large, and the zine ion in zinc-containing water can effectively be removed or reclaim to the advantage of high adsorption capacity.
(2) method of Treatment of Zinc-containing Wastewater of the present invention has the advantage of " low input, high production ".
(3), in the method for Treatment of Zinc-containing Wastewater of the present invention, without adding a large amount of chemical agents, thereby avoided the generation of follow-up refuses such as a large amount of precipitation body refuses.
Preservation information
Classification And Nomenclature: Fusarium oxysporum (Fusarium oxysporum);
Preservation date: on August 16th, 2013;
Depositary institution: China Committee for Culture Collection of Microorganisms's common micro-organisms center (be called for short: CGMCC), address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, 100101;
Deposit number: CGMCC No.8037.
Embodiment
Below by embodiment, further illustrate technical scheme of the present invention.
The separation of embodiment 1 fungal bacterial strain LP-16
The separation of fungal bacterial strain LP-16 of the present invention comprises following steps:
(1) by 10 grams, packed in the triangular flask that contains 90 ml sterile waters by the fresh soil sample of heavy metal contamination;
(2) with granulated glass sphere, break up, stir evenly, make soil supension;
(3) utilize coating method that soil supension is coated on isolation medium flat board, the consisting of of described isolation medium: sodium acetate, anhydrous 0.2406g/L, yeast extract 0.15g/L, agar 15g/L, paraxin 0.1g/L, Zn
2+50mg/L, the MgSO of 0.05g/L
47H
2the K of O and 0.005g/L
2hPO
4, all the other are deionized water, and pH=7.0,120 ℃ of autoclave sterilizations 30 minutes;
(4) be coated with isolation medium is cultivated 2～4 days under the condition of 30 ℃, obtained single bacterial strain;
(5) described single bacterial strain is accessed in fresh described isolation medium and proceeds separation and Culture, obtain the bacterial strain of purifying;
(6) to the operation of the purifying bacterial strain repeating step (5) obtaining in step (5) 3～5 times, take and guarantee the single bacterial strain that resulting bacterial strain is Economical Purification, obtain fungal bacterial strain of the present invention and in 4 ℃ of preservations.
The preparation of embodiment 2 microbial inoculums
The preparation of the microbial inoculum in the present invention comprises following steps:
1) Petri Dishes cultivates: purified original strain (Fusarium oxysporum) is inoculated in respectively on solid medium under aseptic condition, under room temperature (25 ℃) condition, cultivates 5 days; Solid medium has following composition: potato is soaked powder 5g/L, glucose 20g/L, agar 15g/L, paraxin 0.1g/L;
2) first order seed is cultivated: the bacterial classification that step 1) is cultivated is inoculated in respectively liquid nutrient medium under aseptic condition, under room temperature (25 ℃) condition, cultivates 6 days, makes first order seed suspension; Liquid nutrient medium has following composition: potato is soaked powder 5g/L, glucose 20g/L, paraxin 0.1g/L;
3) secondary seed is cultivated: the inoculum size that is 10% by the volume ratio of liquid nutrient medium, first order seed is inoculated into respectively in the fermentor tank of 2.5L, and in fermentor tank, the cumulative volume of nutrient solution is 1.5L, suitably aeration, cultivates 6 days, makes secondary seed;
4) mixing fermentation culture: the inoculum size that is 15% by the volume ratio of liquid nutrient medium, secondary seed is inoculated in the fermentor tank of 10L, the substratum cumulative volume in fermentor tank is 7L, carries out high density fermentation cultivation, obtains microbial inoculum.
The impact of zinc ion concentration on fungi LP-16 absorption zine ion ability in embodiment 3 waste water
Secure ph is 6.0 liquid enlarged culturing base 6 equal portions, and every part of culture volume is 150mL, packs in 250mL triangular flask.In these 6 bottles of solution, contain respectively Zn
2+concentration is 12,24,36,48,60,120mg/L.In these 6 bottles of liquid nutrient mediums, inoculate fungi LP-16 thalline of the present invention, temperature of reaction is 25 ℃, shaking culture 6 hours, and rotating speed is 120RPM, the solution of mycetome is carried out centrifugal, measures Zn in centrifugal rear liquid
2+concentration, observes the degree that thalli growth state and thalline adsorb, fungi LP-16 bacterial strain absorption Zn in the waste water of Different Zinc ionic concn
2+effect as shown in table 1.
The impact of zinc ion concentration on bacterial strain absorption zine ion ability in table 1, waste water
| Zinc ion concentration (mg/L) | Concentration (mg/L) after absorption | Adsorption efficiency |
| 12 | 3.33 | 72.25% |
| 24 | 9.1 | 62.08% |
| 36 | 14.7 | 59% |
| 48 | 27.9 | 41.88% |
| 60 | 29.9 | 50.17% |
| 120 | 68.3 | 43.08% |
Table 1 result shows, along with the increase of zinc ion concentration, this thalline reduces gradually to the absorption of zine ion, and the continuation increase of zinc ion concentration can affect the growth of this bacterial classification on the contrary, then affects the adsorption efficiency to zine ion.
The impact of embodiment 4 thalline dosages on zine ion ability in fungi LP-16 absorption waste water
Secure ph is 6.0 liquid enlarged culturing base, and volume is 150mL, packs in 250mL triangular flask.In solution, contain Zn
2+concentration is 60mg/L.In nutrient solution, add respectively 0.5,1,2,5, the fungi LP-16 thalline of 10g, temperature of reaction is 25 ℃, shaking culture 6 hours, rotating speed 120RPM, cultured bacteria suspension is centrifugal, measure Zn in centrifugal rear liquid
2+concentration, observes the degree that thalli growth state and thalline adsorb, and the effect that under different thalline dosages, fungi LP-16 adsorbs zine ion in waste water is in Table 2.
Table 2, the impact of thalline dosage on bacterial strain absorption zine ion ability
As seen from the results in Table 2, the most economical when dosage is 5g thalline, cost performance is the highest.
Embodiment 5 pH value in reaction reclaim the impact of zine ion ability from waste water on fungi LP-16
Secure ph is respectively 3.0,4.0,5.0,6.0,7.0 liquid enlarged culturing base, and volume is 150mL, packs in 250mL triangular flask.In solution, contain Zn
2+concentration is 60mg/L.The fungi LP-16 thalline of inoculating 2g in nutrient solution, temperature of reaction is 25 ℃, and shaking culture 6 hours, turns 120RPM, and speed is centrifugal by cultured bacteria suspension, measures Zn in centrifugal rear liquid
2+concentration, observes the degree that thalli growth state and thalline adsorb, and the effect of the lower fungi LP-16 absorption of differential responses pH value zine ion is in Table 3.
Table 3, the impact of pH value on bacterial strain absorption zine ion ability
Table 3 result is visible, and bacterial strain is 5.0 o'clock at pH, and adsorption efficiency is the highest, and PH is 6.0 and takes second place for 7.0 o'clock, illustrates: fungi LP-16 is that adsorption efficiency between 5.0-7.0 is best at pH.
The impact of embodiment 6 temperature of reaction on zine ion ability in fungi LP-16 absorption waste water
Secure ph is 6.0 liquid enlarged culturing base, and volume is 150mL, packs in 250mL triangular flask.In solution, contain Zn
2+concentration is 60mg/L, inoculates 2g fungi LP-16 thalline in nutrient solution, and temperature of reaction is respectively 15,20,25,30 ℃, shaking culture 6 hours, and rotating speed 120RPM, cultured bacteria suspension is centrifugal, measure Zn in centrifugal rear liquid
2+concentration, observes the degree that thalli growth state and thalline adsorb, and at differential responses temperature, the effect of fungi LP-16 absorption zine ion is in Table 4.
Table 4, the impact of temperature of reaction on bacterial strain absorption zine ion ability
Table 4 result shows, when temperature of reaction is between 15-30 ℃, fungi LP-16 thalline all has good effect for the absorption of zine ion in waste water, especially between 20-30 ℃, show adsorption efficiency and promote gradually, show in this temperature range, concrete adsorption effect is become better and better.
The impact of embodiment 7 duration of oscillations on zine ion ability in fungi LP-16 absorption waste water
Secure ph is 6.0 liquid enlarged culturing base, and volume is 150mL, packs in 250mL triangular flask.In solution, contain Zn
2+concentration is 60mg/L.The fungi LP-16 thalline of inoculating 2g in nutrient solution, temperature of reaction is 25 ℃, and shaking culture 10min, 30min, 2h, 4h, 6h, turn 120RPM respectively, and speed is centrifugal by cultured bacteria suspension, measures Zn in centrifugal rear liquid
2+concentration, observes the degree that thalli growth state and thalline adsorb, and under the condition of different duration of oscillations, the effect of fungi LP-16 absorption zine ion is in Table 5.
Table 5, the impact of different duration of oscillation on bacterial strain absorption zine ion ability
Table 5 result shows, in the scope that is 10min-6h at duration of oscillation, the adsorption effect of thalline is better; Particularly, when the earthquake time is 2h, adsorption efficiency is the highest, shows that thalline adsorption effect is now best.
Applicant statement, the present invention illustrates of the present invention by above-described embodiment, but that the present invention is not limited to is above-mentioned, does not mean that the present invention must rely on above-mentioned could enforcement.Person of ordinary skill in the field should understand, any improvement in the present invention, to the selection of the interpolation of the equivalence replacement of the selected raw material of the present invention and ancillary component, concrete mode etc., within all dropping on protection scope of the present invention and open scope.
Claims (9)
1. a fungal bacterial strain LP-16 (Fusarium oxysporum), is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), and preservation date is on August 16th, 2013, and deposit number is CGMCC No.8037.
2. for separating of an isolation medium of fungal bacterial strain LP-16 as claimed in claim 1, it is characterized in that, described isolation medium comprises 0.1～0.4g/L sodium acetate, 0.05～0.2g/L yeast extract, 5～25g/L agar, 40～60mg/L Zn
2+, 0.01～0.12g/L MgSO
47H
2o, 0.001～0.010g/L K
2hPO
4with 0.05～0.2g/L paraxin, and pH=7.0;
Preferably, described isolation medium comprises 0.2～0.3g/L sodium acetate, 0.1～0.2g/L yeast extract, 10～20g/L agar, 45～55mg/L Zn
2+, 0.03～0.1g/L MgSO
47H
2o, 0.003～0.008g/L K
2hPO
4with 0.08～0.15g/L paraxin, and pH=7.0;
More preferably, described isolation medium comprises 0.2461g/L sodium acetate, 0.15g/L yeast extract, 15g/L agar, 50mg/L Zn
2+, 0.05g/L MgSO
47H
2o, 0.005g/L K
2hPO
4with 0.1g/L paraxin, and pH=7.0.
3. isolation medium according to claim 2, is characterized in that, also adds 2ml trace element solution in isolation medium every liter described, and described trace element solution comprises 1～5g/L CaCl
22H
2o, 1～5g/L2.5g H
3bO
3, 0.5～1.2g/L ZnCl
24H
2o, 0.5～1.5g/L FeCl
36H
2o, 0.3～0.6g/L ZnSO
47H
2o, 0.1～0.4g/L Na
2moO
42H
2o and 0.003～0.008g/LCuSO
45H
2o;
Preferably, described trace element solution comprises 2～4g/L CaCl
22H
2o, 1～3g/L H
3bO
3, 0.7～1g/LZnCl
24H
2o, 0.8～1.2g/L FeCl
36H
2o, 0.4～0.5g/L ZnSO
47H
2o, 0.2～0.3g/LNa
2moO
42H
2o and 0.005～0.006g/L CuSO
45H
2o;
More preferably, described trace element solution comprises 3.7g CaCl
22H
2o, 2.5g H
3bO
3, 0.87gZnCl
24H
2o, 1.0g FeCl
36H
2o, 0.44g ZnSO
47H
2o, 0.29g Na
2moO
42H
2o and 0.005g CuSO
45H
2o.
4. a fungal inoculant, is characterized in that, comprises fungal bacterial strain LP-16 claimed in claim 1.
5. the preparation method of fungal inoculant as claimed in claim 4, is characterized in that, described method comprises:
(1) culture dish is cultivated: fungal bacterial strain LP-16 as claimed in claim 1 is inoculated on solid medium under aseptic condition to 20-30 ℃, preferably 22-28 ℃, further preferably cultivate at 25 ℃ 4-6 days, preferably 4.4-5.5 days, further preferably 5 days; Described solid medium comprises following component: potato is soaked powder 4-6g/L, preferred 4.5-5.5g/L, further preferred 5g/L, glucose or sucrose 15-25g/L, preferred 17-23g/L, further preferred 20g/L, agar 13-18g/L, preferred 14-17g/L, further preferred 16g/L, paraxin 0.1g/L;
(2) first order seed is cultivated: the bacterial classification that step (1) is cultivated is inoculated in liquid nutrient medium under aseptic condition, 20-30 ℃, and preferred 22-28 ℃, further preferably cultivates 5-7 days at 25 ℃, and preferably 5.5-6.5 days, more preferably 6 days, makes first order seed; Described liquid nutrient medium comprises following component: potato is soaked powder 4-6g/L, preferred 4.5-5.5g/L, further preferred 5g/L, glucose or sucrose 15-25g/L, preferred 17-23g/L, further preferred 20g/L, paraxin 0.1g/L;
(3) secondary seed is cultivated: by the volume ratio of liquid nutrient medium, be 5-15%, preferably 8-12%, more preferably 10% inoculum size, step (2) gained first order seed is inoculated in fermentor tank, aeration, cultivates 5-7 days, preferably 5.5-6.5 days, further preferably 6 days, makes secondary seed;
(4) mixing fermentation culture: be 10-20%, preferably 12-18%, more preferably 15% inoculum size by the volume ratio of liquid nutrient medium, secondary seed is inoculated in fermentor tank, carry out high density fermentation cultivation, obtain microbial inoculum.
6. a zine ion treatment agent, is characterized in that, described zine ion treatment agent comprises fungal bacterial strain LP-16 as claimed in claim 1; Preferably, described in, be treated to and remove or reclaim.
7. the method for a Treatment of Zinc-containing Wastewater, it is characterized in that, described method comprises: fungal bacterial strain LP-16 as claimed in claim 1, fungal inoculant as claimed in claim 4 or zine ion treatment agent as claimed in claim 6 are contacted with described zinc-containing water.
8. method as claimed in claim 7, is characterized in that, described method comprises:
(1) the pH value of zinc-containing water is adjusted to 5.0-8.0, preferred 5.5-7, further preferably 6.0;
(2) in water body, add fungi as claimed in claim 1, fungal inoculant as claimed in claim 4 or zine ion treatment agent as claimed in claim 6, in 10-35 ℃, preferred 15-30 ℃, further preferably 25 ℃, concussion 4-8 hour, preferably 5-7 hour, further preferably 6 hours, concussion speed 90-180rpm, preferred 100-160rpm, further preferred 120rpm;
(3) standing 8-16 hour, preferred 10-14 hour, further preferably 12 hours.
9. method as claimed in claim 7 or 8, the zine ion in described zinc-containing water is removed or reclaimed to wherein said being treated to.
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| CN104560736A (en) * | 2015-01-08 | 2015-04-29 | 中节能六合天融环保科技有限公司 | Fungi strain GX-4 and application thereof in mercury-containing wastewater treatment |
| CN105585136A (en) * | 2016-01-20 | 2016-05-18 | 中南大学 | Method for treating zinc-containing wastewater by utilizing granular sludge formed through microbiological flocculation |
| CN105692913A (en) * | 2016-01-20 | 2016-06-22 | 中南大学 | Method for treating copper-containing wastewater by forming granular sludge through microorganism sediment |
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| CN102277302A (en) * | 2011-06-15 | 2011-12-14 | 上海交通大学 | Construction method for combining trichoderma and brassica napobrassica for absorbing farmland soil heavy metals cadmium and zinc |
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| CN102277302A (en) * | 2011-06-15 | 2011-12-14 | 上海交通大学 | Construction method for combining trichoderma and brassica napobrassica for absorbing farmland soil heavy metals cadmium and zinc |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104560736A (en) * | 2015-01-08 | 2015-04-29 | 中节能六合天融环保科技有限公司 | Fungi strain GX-4 and application thereof in mercury-containing wastewater treatment |
| CN105585136A (en) * | 2016-01-20 | 2016-05-18 | 中南大学 | Method for treating zinc-containing wastewater by utilizing granular sludge formed through microbiological flocculation |
| CN105692913A (en) * | 2016-01-20 | 2016-06-22 | 中南大学 | Method for treating copper-containing wastewater by forming granular sludge through microorganism sediment |
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