CN103589677B - The application of fatty acid methyl ester compound - Google Patents
The application of fatty acid methyl ester compound Download PDFInfo
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- CN103589677B CN103589677B CN201310520864.1A CN201310520864A CN103589677B CN 103589677 B CN103589677 B CN 103589677B CN 201310520864 A CN201310520864 A CN 201310520864A CN 103589677 B CN103589677 B CN 103589677B
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- fatty acid
- acid methyl
- methyl ester
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- ester compound
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Abstract
The application of fatty acid methyl ester compound; the compounds of this invention is separated from waterplant duckweed root exudates; there is good nitrogen and remove promotion activity; under low dosage, effectively can improve the nitric efficiency of common denitrifying bacterium Pseudomonas fluorescens, agriculture environmental protection can be used as the purifying potentiating agent of azotate pollution water body.
Description
Technical field
The invention belongs to eutrophication water Pollution control technology research field, be specifically related to a kind of application that can promote the fatty acid methyl ester compound of denitrifying microorganism denitrification activity.
Background technology
First time in 2010, national Pollutant source investigation publication showed, the azotate pollution of agricultural sources has exceeded the main source that industrial source becomes the total polluted by nitrogen of China's water body, causes river and water body in lake eutrophication, causes surface water pollution, serious threat HUMAN HEALTH.In the long run/term, excessive nitrate can cause the change of whole ecologic community structure and diversity.Therefore, the purifying treatment of agricultural effluent and rich water has become the new study hotspot of water treatment field at present.Utilization has efficient Nitrogen Absorption ability and maybe can promote that the waterplant that microorganism is denitrogenated processes by the agricultural effluent of azotate pollution, is a kind of effective biopurification means.
Duckweed is a kind of small-sized floating plant that is seen everywhere in area, farmland.Have been reported and show, duckweed removes the denitrification process mainly promoting microorganism to the acceleration of water body nitrogen, instead of own absorption ability is strong.Root system of plant, by secreting specific compound, changes denitrification activity and the structure of community of rhizospheric microorganism, thus promotes that microorganism is denitrogenated.Therefore, the rhizosphere effect of fortification of plants-microorganism, as added the specific compound that can stimulate denitrifying microorganism denitrogenation, the conversion that will speed up Water element is removed, for the biologic technology building efficient process farmland drainage nitrogen provides new method and thinking.
In the farmland water body that nitrate is sufficient but organic content is low, low C/N is restricted than making the activity of denitrifying microorganism.At present, the carbon compound that can improve water body denitrification that great majority add, as saccharide compound (as glucose and fructose etc.), methyl alcohol and acetic acid etc., Main Function is for denitrifying microorganism provides nutrition and accelerate growth of microorganism, if improper use, the raised growth of microorganism can cause water quality deterioration on the contrary.And these compounds easily run off and are decomposed by the microorganisms in soil and water, and methyl alcohol etc. also have toxicity.So far, also there is not report plant-sourced can accelerate the compound of the specific non-nutritive that microorganism is denitrogenated, and this kind of " green " material plays prior effect at plant-microorganism rhizosphere Interaction, conscientiously can improve the activity of denitrifying microorganism, in eutrophication water Environmental capacity field, there is more wide application prospect.
Summary of the invention
The technical problem solved:
Technical problem to be solved by this invention is to provide a kind of application that efficiently can promote the fatty acid methyl ester compound of denitrifying microorganism denitrogenation.This substance is by promoting that the activity of denitrifying microorganism improves water body nitrogen removal efficiency, fortification of plants-microorganism rhizosphere effect, with simple method solve in water pollution control process low C/N farmland drainage time nitric efficiency low technical barrier, material of the present invention also can improve the problem that additional carbon easily runs off and is decomposed by the microorganisms simultaneously, improve denitrifying microorganism to the nitric efficiency of farmland drainage, save the cost of additional carbon.
Technical scheme:
Fatty acid methyl ester compound
[Methyl (Z)-7-hexadecenoate, cis-7-HDM] application in the denitrification activity improving denitrifying microorganism.
Fatty acid methyl ester compound
accelerating the application in the removal of water body denitrifying microorganism nitrogen.
Described denitrifying microorganism is Pseudomonas fluorescens.
Screen duckweed spirodela (Spirodela polyrrhiza) to TAI HU AREA to collect with blue or green duckweed (Lemna minor) root exudates, be separated and biological activity assay, and GC/MS qualification is carried out to active ingredient F.The while of in these two kinds of duckweed root exudates active ingredients, isolation identification is to 4 kinds of fatty acid methyl Esters, wherein methyl (Z)-7-hexadecenoate(Cis-7-HDM, molecular weight: 268.43) have significant nitrogen and remove facilitation effect.
Present invention also offers above-claimed cpd and promote the application in the denitrogenation of denitrification specified microorganisms.Described denitrifying microorganism is common Pseudomonas fluorescens.This promotor is 5-60mg L in interpolation concentration
-1time, the facilitation effect of the 5-25% that can have an appointment to Pseudomonas fluorescens denitrification percent.
Non-nutritive compound of the present invention is main in Microbial denitrogenation process plays signal function, facilitates the activity of denitrifying microorganism, instead of simply provides carbon source to promote the growth of denitrifying microorganism.
Beneficial effect:
(1) the compounds of this invention efficiently can promote the activity of denitrifying microorganism under low dosage, avoids the water quality deterioration phenomenon causing other in actual application because promoting microorganism raised growth;
(2) the compounds of this invention is a kind of fat-soluble cpds, not easily runs off in soil and water, can ensure stability and the high efficiency of application;
(3) the compounds of this invention is a kind of " green " denitrogenation promotor, can solve the carbon source of conventional nutritious effect in inefficient problem in application of purifying waste water, the biologic technology simultaneously for building efficient process farmland drainage nitrogen provides new method and thinking.
Accompanying drawing explanation
Fig. 1 is the GC/MS analysis of spectra of duckweed root exudates active ingredient F of the present invention.Accelerant C is-7-HDM of the present invention is the material that 20.04min goes out peak, and other 3 kinds is other fatty acid methyl ester compound identified;
Fig. 2 is that denitrogenation of the present invention promotes that material compares (mean ± SE, n=3) with the promotion rate of other materials in active ingredient, and between different lowercase expression groups, there were significant differences (P<0.05, LSD test);
Fig. 3 is that denitrogenation of the present invention promotes dosages of substance-Pseudomonas fluorescens nitric efficiency promotion rate relation curve (mean ± SE, n=3).
Embodiment
The following examples can make the present invention of those skilled in the art comprehend, but do not limit the present invention in any way.
Embodiment 1 efficiently promotes the effectiveness comparison between the fatty acid methyl ester compound that nitrogen is removed and allied substances
1.1 experimental design
(1) standard substance: the four kinds of fatty acid methyl ester compound identified in root exudates active ingredient, Methylhexadecanoate(HDM), methyl dodecanoate(DDM) and methyl12-hydroxystearate(12-HSM) be purchased from Sigma-Aldrich company (St.Louis, MO, USA) .Methyl (Z)-7-hexadecenoate(Cis-7-HDM) be purchased from Cayman Chemical Co company (Michigan, USA), these working substances are all dissolved in CH
2cl
2in.
(2) microorganism strains: Pseudomonas fluorescens (ACCC01047), is purchased from Chinese agriculture Microbiological Culture Collection administrative center.
(3) LB substratum: 10g/L tryptone, 5g/L yeast extract, 10g/L NaCl, pH7.0.
(4) denitrification substratum: 0.72g/L KNO
3, 1.0g/L KH
2pO
4, 0.20g/L MgSO
47H
2o, 2.8g/LC
4h
4na
2o
46H
2o, pH7.0.
(5) biological activity test
Be collected in the Pseudomonas fluorescens activating 20h in LB nutrient solution, centrifugal 10min (5,000g, 4 DEG C), then Eddy diffusion is in aseptic denitrification nutrient solution.1mL Pseudomonas fluorescens bacterium liquid and each working substance of 20 μ L, add in the 50mL triangular flask containing 19mL denitrification nutrient solution, makes the concentration of final working substance be 20mg L
-1, in shaking table 30 DEG C, 120rpm cultivates.Contrast is interpolation 20 μ L CH
2cl
2, Setup Experiments 3 repetition.After cultivating 72h, draw 1mL bacterium liquid centrifugal 10min(10,000g), supernatant liquor measures total nitrogen content (alkaline chitinase clears up ultraviolet spectrophotometry).The biomass turbidimetry for Determination of bacterium liquid, namely uses spectrophotometer (Bio-Rad3000; USA) thalline is measured in 600nm place absorbancy.The method of calculation of Pseudomonas fluorescens denitrification percent (%) and promotion rate (%) are as follows:
(6) data analysis
Data processed result all represents with (mean ± SE), adopts SPSS18.0 statistical software to carry out comparing between two between one-way analysis of variance and group.
1.2 experimental result
Carry out GC/MS analysis to duckweed root exudates active ingredient, identify 4 kinds of fatty acid methyl ester compound, the material going out peak at 20.04min is accelerant C is-7-HDM(Fig. 1 of the present invention).By 4 kinds of working substance facilitation effect counterevidence experiments, find that the different substances of same class compound has different impacts (table 1) to Pseudomonas fluorescens denitrification percent.At interpolation concentration 20mgL
-1after accelerant C is-7-HDM process of the present invention, have significant promoter action (p<0.05) to the denitrification percent of Pseudomonas fluorescens, promotion rate is about 20.5%(Fig. 2).But after other 3 kinds of working substances add, the denitrification percent of Pseudomonas fluorescens is not made significant difference.And there were significant differences (Fig. 2) for the promotion rate of Cis-7-HDM and other 3 kinds of working substances.Result shows, the denitrification percent of accelerant C is-7-HDM of the present invention to Pseudomonas fluorescens has specific facilitation effect.
Relative to contrast, the interpolation of these 4 kinds of working substances on the growth of Pseudomonas fluorescens substantially without impact, the OD of each process
600value is all at about 0.45 (table 1).Visible, promotor of the present invention mainly improves the denitrification activity of Pseudomonas fluorescens.
In addition, owing to adding 20mg L
-1cis-7-HDM is in denitrification nutrient solution, its total organic carbon concentration (TOC) accounts for the content lower than 2% relative to TOC in denitrification nutrient solution, therefore, accelerant C is-7-HDM of the present invention mainly plays intracellular signaling effect and activates denitrification activity, instead of simply provides the carbon source of denitrifying microorganism to promote that it grows.
Between * different lowercase expression groups, there were significant differences (P<0.05, LSD test)
Embodiment 2 accelerant C is-7-HDM is to the promoter action of Pseudomonas fluorescens nitric efficiency
2.1 experimental design
Add 5,10,20,40,60mg L respectively
-1accelerant C is-7-HDM of the present invention in denitrification nutrient solution, then add 1mL Pseudomonas fluorescens bacterium liquid, in shaking table 30 DEG C, 120rpm cultivates, and contrasts as CH
2cl
2.Cultivate after 72h, bacterium liquid is centrifugal according to method in 1.1, and supernatant liquor measures total nitrogen concentration, calculates the denitrogenation promotion rate (%) after each process.
2.2 experimental result
Promotor of the present invention is 5-60mg L in interpolation concentration
-1time, the facilitation effect (Fig. 3) of 5-25% that the denitrification percent of Pseudomonas fluorescens is had an appointment.Owing to adding 40mg L
-1during concentration, denitrogenation promotion rate reaches maximum, so consider cost, advises that the addition of Cis-7-HDM in application process is 40mg L
-1left and right, the dosage simultaneously for being applied to other denitrification bacterial strains is for referencial use.
Claims (2)
1. cis-structure fatty acid methyl ester compound
application in the denitrification activity improving Pseudomonas fluorescens (P.Fluorescens).
2. cis-structure fatty acid methyl ester compound
acceleration water body Pseudomonas fluorescens to the application in nitrate removal.
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| CN103314973A (en) * | 2013-06-13 | 2013-09-25 | 广西壮族自治区海洋研究所 | Method for extracting anti-artemia active ingredients from styela canopus |
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Non-Patent Citations (7)
| Title |
|---|
| Aerobic denitrifying characteristics of duckweed rhizosphere bacterium RWX31;Zhou YingRu, Shi Weiming, 等;《African Journal of Microbiology Research》;20130731;第7卷(第3期);211-219 * |
| Antioxidant and antimicrobial activities of essential oil and extracts of Saurauia lantsangensis hu root;Zhu L, 等;《Z Naturforsch C》;20120630;第67卷(第5-6期);282-290 * |
| Mechanism of fatty acid desaturation in the green alga Chlorella vulgaris;Behnaz Behrouzian;《Eur. J. Biochem.》;20011231;第268卷;3545-3549 * |
| 太湖地区高效除氮浮萍品种的筛选及其除氮机理的初步研究;周影茹, 施卫明，等;《土壤》;20100615;第42卷(第3期);390-397 * |
| 好氧反硝化菌RWX31强化污染废水脱氮的研究;周影茹, 施卫明, 等;《农业环境科学学报》;20131031;第32卷(第10期);2047-2054 * |
| 环太湖地区农田排水高效除氮浮萍品种的筛选及其除氮机理的研究;周影茹;《中国优秀硕士学位论文全文数据库》;20100501;摘要 * |
| 龙脑樟鲜叶挥发油成分及其抗菌活性的研究;丁雄, 等;《食品工业科技》;20121231;第33卷(第18期);167-171 * |
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