CN103583435B - Eel leucomalachite-green-removal aquaculture method - Google Patents
Eel leucomalachite-green-removal aquaculture method Download PDFInfo
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- CN103583435B CN103583435B CN201310565384.7A CN201310565384A CN103583435B CN 103583435 B CN103583435 B CN 103583435B CN 201310565384 A CN201310565384 A CN 201310565384A CN 103583435 B CN103583435 B CN 103583435B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
An eel leucomalachite-green-removal aquaculture method comprises the following steps that the diameter of an ecological fishpond is 2-10 meters, the depth of water is 1.5 meters, the volume of the water is 4.7-25 cubic meters, 250-1250 kilograms of fishes are placed in the fishpond, and the fishes are bred in the environment at the temperature of 20-30 DEG C; the fishes are bred for 10 days, no fodder or drug is fed, eels lose weight by 10% after 10 days, and 225-1125 kilograms of the fresh and alive eels are obtained; micro-cluster water and air are mixed and fed into the fishpond; the micro-cluster water and the air are fed for 0.5 hour, in addition, ordinary water is fed slowly for 1.5 hours, and the water in the fishpond flows slowly; operation is conducted in this way alternately, and 24-hour automatic operation is conducted; the fishes are bred for 8-12 days. According to the eel leucomalachite-green-removal aquaculture method, the micro-cluster water with the high content of reactive oxygen enters cells, eucomalachite green is oxidized into malachite green, the malachite green is brought out of the cells and discharged outside bodies, and the eels reach an export standard.
Description
Technical field
The present invention relates to a kind of cultural method, particularly eel leucomalachite-greenaquaculture aquaculture method.
Background technology
Malachite green is a kind of organic compound of Prof. Du Yucang.Because of its color and natural malachite color similarity and gain the name.
Since 1933 find that malachite green has the drug effects such as antibacterial, desinsection, many countries are extensively in order to killing water product parasite, mould etc.
In fish somatic cell under reductase catalysis, malachite green is reduced to leucogentian violet and leucogentian violet in eel body cannot be got rid of, and is the lifelong residue of eel.
The easy demethylation of leucogentian violet forms the structure similar in appearance to benzidine, and benzidine is the first carcinogenic substance, and its structure is easy to the base replaced in DNA and matches, and causes DNA genetic transcription to suddenly change.Here it is, and malachite green has carcinogenic, teratogenesis, mutagenic reason, and the aquatic products that the mankind have eaten band leucogentian violet are very harmful.
National agricultural portion issued " emergency notice investigating and prosecuting forbidding veterinary drugs such as " malachite greens " about tissue " and checked illegal business in China severely on July 7th, 2005, used the behavior of " malachite green ".But owing to also there is no effective substitute at a low price.Malachite green aquaculture, transport with the process of peddling in remain incessant after repeated prohibition.
Summary of the invention
Technical solution of the present invention, in fish somatic cell, 80% is water, is namely medium with water.Malachite green positively charged, for water-soluble, is easily entered in cell by water-band.But in cell reductase catalysis under, be converted into the malachite green that uncharged neutrality is colourless, or claim leucogentian violet.And leucogentian violet is no longer water-soluble, therefore extracellular cannot be gone out by water-band, namely cannot remove.As removed, have only and leucogentian violet is oxidized to malachite green.Under the association parcel of water, could extracellular be discharged, and release external.
Malachite green is reduced to leucogentian violet under reductase catalysis:
The easy demethylation of leucogentian violet forms the structure similar in appearance to benzidine:
As everyone knows, benzidine is the first carcinogen, and its structure is as follows:
Again because the ion channel diameter of cell membrane is only 2 nanometers, only has small-micelle water (0.5---0.8 nanometer) just by ion channel in cell membrane.
Meanwhile, small-micelle water enters in cell with active oxygen, just leucogentian violet can be oxidized back malachite green, and discharge extracellular, excrete.
Eel leucomalachite-greenaquaculture aquaculture method, comprises the steps:
1) Ecological Fish Culture pond diameter 2-10 meters, the depth of water 1.5 meters, water is 4.7-25 cubic metres, puts fish 500-2500 jin, puts in a suitable place to breed under the environment of 20-30 degrees Celsius;
2) time of breeding fish is 10 days, do not throw something and feed any feed and medicine, eel weight reducing 10% after 10 days, i.e. 450-2250 jin of fresh and alive eels;
3) small-micelle water is mixed with air pass in pond;
4) setting the water flowing aerating time is 0.5 hour, within other 1.5 hours, slowly passes into light water, keeps the water in pond slowly to flow;
5) water flowing aerating and slow water flowing, hocket according to this, and running automatically in 24 hours, the time of breeding fish continues 8-12 days again.
As a further improvement on the present invention: the small-micelle water passed into is 0.5-0.8 nanometer.
As a further improvement on the present invention: small-micelle water enters in cell with active oxygen, leucogentian violet being oxidized back malachite green gets rid of external.
As a further improvement on the present invention: small-micelle water water inlet pipe and oxygen increasing tube mutually also, make water oxygen mix and enter in ecological culture pond together.
Compared with prior art, the invention has the beneficial effects as follows: adopt the high small-micelle water containing active oxygen to enter in cell, leucogentian violet is oxidized to malachite green, takes extracellular out of, excrete, make eel reach export standard.
Embodiment
Eel leucomalachite-greenaquaculture aquaculture method, comprises the steps:
1) Ecological Fish Culture pond diameter 2-10 meters, the depth of water 1.5 meters, water is 4.7-25 cubic metres, puts fish 500-2500 jin, puts in a suitable place to breed under the environment of 20-30 degrees Celsius;
2) time of breeding fish is 10 days, do not throw something and feed any feed and medicine, eel weight reducing 10% after 10 days, i.e. 450-2250 jin of fresh and alive eels;
3) small-micelle water is mixed with air pass in pond;
4) setting the water flowing aerating time is 0.5 hour, within other 1.5 hours, slowly passes into light water, keeps the water in pond slowly to flow;
5) water flowing aerating and slow water flowing, hocket according to this, and running automatically in 24 hours, the time of breeding fish continues 8-12 days again.
The small-micelle water passed into is 0.5-0.8 nanometer.
Small-micelle water enters in cell with active oxygen, leucogentian violet is oxidized back malachite green and gets rid of external.
Small-micelle water water inlet pipe and oxygen increasing tube mutually also, make water oxygen mix and enter in ecological culture pond together.
operating procedure is as follows:
the present invention adopts mature technology that water is become small-micelle water.Small-micelle water water inlet pipe and oxygen increasing tube mutually also, make water oxygen mix and enter in ecological culture pond together.Allow eel move, accelerate metabolism, accelerate excretion.
Once in another monograph (patent No. is 201320189322X) " a kind of original ecological breeding fishpond cylinder assembly ", cultivated eel after 10 days with common clear water, test, result is as follows:
| Inspection item | Testing result | Unit | Detection method |
| Malachite green | <0.5* | ug/kg | GB/T 19857-2005 |
| Leucogentian violet | 8.4 | ug/kg | GB/T 19857-2005 |
| Cistofuran metabolite | <0.5* | ug/kg | FIC B074-2004 |
| Furacilin metabolite AHD | <0.5* | ug/kg | FIC B074-2004 |
| AMOZ AMOZ | <0.5* | ug/kg | FIC B074-2004 |
| Furaxone metabolite AOZ | <0.5* | ug/kg | FIC B074-2004 |
| Chloramphenicol | <0.1* | ug/kg | GB/T 20756-2006 |
* detect and track is represented
Result shows: other medicine and malachite Green City are got rid of, but leucogentian violet is still high-load, is difficult to remove.
Be placed on a collection of eel in same ecological pool, cultivate 10 days again with the oxygen containing small-micelle water of height, send same testing agency to carry out leucogentian violet special project and detect, its result is as follows:
| Inspection item | Testing result | Unit | Detection method |
| Malachite green | <0.5* | ug/kg | GB/T 19857-2005 |
| Leucogentian violet | <0.5* | ug/kg | GB/T 19857-2005 |
* detect and track is represented
Result shows, leucogentian violet is got rid of, and eel reaches export standard.
In sum, after those of ordinary skill in the art reads file of the present invention, make other various corresponding conversion scheme according to technical scheme of the present invention and technical conceive without the need to creative mental labour, all belong to the scope that the present invention protects.
Claims (2)
1. eel leucomalachite-greenaquaculture aquaculture method, is characterized in that comprising the steps:
1) Ecological Fish Culture pond diameter 2-10 meters, the depth of water 1.5 meters, water is 4.7-25 cubic metres, puts fish 500-2500 jin,
Put in a suitable place to breed under the environment of 20-30 degrees Celsius;
2) time of breeding fish is 10 days, do not throw something and feed any feed and medicine, eel weight reducing 10% after 10 days, i.e. 450-2250 jin of fresh and alive eels;
3) small-micelle water is mixed with air pass in pond; Small-micelle water water inlet pipe and oxygen increasing tube mutually also, make water oxygen mix and enter in ecological culture pond together; Small-micelle water enters in cell with active oxygen, leucogentian violet is oxidized back malachite green and gets rid of external;
4) setting the water flowing aerating time is 0.5 hour, within other 1.5 hours, slowly passes into light water, keeps the water in pond slow
Slow flow;
5) water flowing aerating and slow water flowing, hocket according to this, and running automatically in 24 hours, the time of breeding fish continues 8-12 days again.
2. according to the eel leucomalachite-greenaquaculture aquaculture method described in claim 1, it is characterized in that: little point that passes into
Son group water is 0.5-0.8 nanometer.
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104012460A (en) * | 2014-06-22 | 2014-09-03 | 上海申航热能科技有限公司 | High-density cultivation system using microcluster water and aeration |
| CN104012461A (en) * | 2014-06-23 | 2014-09-03 | 上海申航热能科技有限公司 | Culture pond utilizing small molecular group water circulation |
| CN105746419B (en) * | 2016-04-27 | 2019-04-23 | 广东黄沙渔业基地有限公司 | A kind of weight reducing fishpond pool cultivation apparatus and its cultural method |
| CN106172091B (en) * | 2016-06-30 | 2019-05-28 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of method that compartment application chlorine dioxide improves fish slimming effect |
| CN106106283B (en) * | 2016-06-30 | 2019-04-05 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of method that compartment application ozone improves fish slimming effect |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US5397466A (en) * | 1993-06-18 | 1995-03-14 | Mytrex Industries, Inc. | Circulating filter and aerator system for use in aquaculture |
| CN1108747C (en) * | 1998-12-09 | 2003-05-21 | 中国科学院南京地理与湖泊研究所 | Artificial breeding technology for dark-figured oriental porpoise |
| CN1202708C (en) * | 2001-12-14 | 2005-05-25 | 辽宁省淡水水产研究所 | Large-scale artificial breeding technology for yellow cartfish |
| CN1283142C (en) * | 2004-09-30 | 2006-11-08 | 天津市换新水产良种场 | Method and device for catfish artificial propogation and fertilized egg circular path hatching |
| JP2010088315A (en) * | 2008-10-06 | 2010-04-22 | Aquatech Japan Inc | Water treatment apparatus for aquaculture pond |
| CN102630605A (en) * | 2011-02-12 | 2012-08-15 | 于雪松 | Research and breeding preparation method of fishes without fishy smell |
| CN102217569B (en) * | 2011-05-05 | 2012-11-21 | 燕山大学 | Additive for removing earthy taste and off-flavor of fish and preparation method thereof |
| CN103353495B (en) * | 2011-11-15 | 2014-11-26 | 南昌大学 | Method for enriching leucomalachite green and leucogentian violet in aquatic products |
| CN202738608U (en) * | 2012-05-17 | 2013-02-20 | 鹤山市雷迅新农机械科技有限公司 | Energy waterer for small chicken farm |
| CN102980961B (en) * | 2012-12-19 | 2014-06-25 | 福州大学 | Pretreatment method of detection on malachite green, crystal violet and residues of malachite green and crystal violet in eels |
| CN103105386B (en) * | 2013-01-29 | 2015-05-20 | 福州大学 | Method for detecting malachite green in water body and aquatic products |
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Effective date of registration: 20210422 Address after: 510000 405, No.1 Guinan, Mingzhu Road, Qifu Road, Yuncheng street, Guangzhou City, Guangdong Province Patentee after: Guangdong Heji Green Agricultural Technology Co.,Ltd. Address before: 510000 Guangdong city of Guangzhou province Zengcheng West Lake Zhengguo Town Beach No. 86 101 Patentee before: GUANGZHOU ZHENGYUAN BIO-AGRICULTURE Co.,Ltd. |