CN103558374A - Quick pesticide residue detector with current-mode immunosensor - Google Patents
Quick pesticide residue detector with current-mode immunosensor Download PDFInfo
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- CN103558374A CN103558374A CN201310576569.8A CN201310576569A CN103558374A CN 103558374 A CN103558374 A CN 103558374A CN 201310576569 A CN201310576569 A CN 201310576569A CN 103558374 A CN103558374 A CN 103558374A
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- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
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- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
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Abstract
The invention relates to a quick pesticide residue detector with current-mode immunosensor. The quick pesticide residue detector consists of the immunosensor, a signal detecting and processing system, a displaying and printing storage system, and a power supply system. A pesticide antibody is fixed on a working electrode surface modified by a grapheme-multiwall carbon-nano-colloidal gold-chitosan nanocomposite so as to prepare the immunosensor, and a three-electrode system is used for detecting the concentration of pesticides according to the change of current of the immunosensor before and after the immunosensor contacts the pesticides. The signal processing circuit is used for carrying out I/V conversion, amplification, filtering and A/D conversion on collected weak current signals, and digital quantity signals are finally sent to a microcontroller for program processing; the detector can display, store and print the result indicating whether the pesticide residue amount in a detected sample exceeds the standard and the concentration value of pesticide residues. The quick pesticide residue detector disclosed by the invention can accurately and quickly detect whether some pesticide is contained in the detection sample and whether the pesticide exceeds the standard, and is extremely suitable for occasions needing quick detection on pesticide residues on site.
Description
Technical field
The present invention relates to a kind of rapid detector for pesticide residue, relate in particular to a kind of current mode immunosensor rapid detector for pesticide residue, belong to agricultural product security detection technique field.
Background technology
The pesticide variety of applying in production field now has reached kind more than 100, to agricultural, keeps good harvest volume increase to play a significant role, and it has become important a kind of means of production.Be accompanied by deeply carrying out of reform and opening-up, in some Changshu Aeras, it is very fast that the speed of development of agricultural chemicals cause becomes.Because mechanization of agriculture constantly advances, it is indispensable that the large area of agricultural chemicals is used.But the irrational use because of agricultural chemicals, has caused many environmental problems, as destroyed in farmland ecosystem etc.A large amount of uses of agricultural chemicals, when having guaranteed a large amount of stable yields of the crops such as grain, have also brought many environmental problems.In plant, soil and water, residual agricultural chemicals has two kinds of remaining forms: the first keeps original chemical constitution; The product of the second after with chemical conversion or to be biodegradable the form of product remaining.Therefore, Pesticide Residue can not be ignored.
The main method of pesticide residue analysis is gas chromatograph, liquid chromatograph, gas chromatograph-mass spectrometer, LC-MS instrument etc. at present, although these method analysis precisions are high, quantitatively accurately, but the complex pretreatment of its sample, detect the operating personnel that length consuming time, cost are high, need to be skilled in technique.The fast determining method of China's residues of pesticides is that enzyme suppresses test paper method and enzyme inhibition-spectrophotometry (the residual fast detector of agriculture), can realize the field quick detection of organophosphorus pesticide and carbamate chemicals for agriculture, has good practical value.Quick measuring card is by the change color of visual inspection card, therefore generally can only carry out observational measurement for the vegetable sample of severe overweight.The application of enzyme inhibition-spectrophotometry is also more extensive, and the residual tacheometer of domestic existing Multiple Pesticides is all based on this principle.Light splitting light ratio juris is that the variation based on absorbance detects, but in vegetable and fruit, a large amount of pigments can cause very large impact to spectrophotometric method, causes the inaccurate of testing result.And said method exists, and the recovery is low, false retrieval, undetected ratio are higher, poor repeatability, be difficult to the shortcomings such as requirement that meet low-residual and quantitatively detect.
The object of invention is for the deficiencies in the prior art, design provides a kind of current mode immunosensor rapid detector for pesticide residue, have easy to carry, detect quick and precisely, simple to operate, can specific determination agricultural product in certain residues of pesticides whether exceed standard and concentration information.
The ultimate principle of a kind of current mode immunosensor of the present invention rapid detector for pesticide residue is: three-electrode system will cause the variation of low current signal due to immune response before and after immunosensor exposed to pesticides, the current signal that testing circuit produces immunosensor gathers.Testing circuit carries out I/V conversion by the low current signal collecting, amplifies, and filtering, A/D conversion the most at last digital quantity signal is sent into single-chip microcomputer and is carried out routine processes.Finally, show and print storage system by parameter displays such as the concentration of residues of pesticides, inhibition degree out, and data being stored so that user checks in time and carries out data analysis.Detector of the present invention includes mini-printer, data can be carried out to corresponding printout.The single-chip microcomputer of input and disposal system inside carries out programming Control to showing with the unification of printing storage system.
For realizing above function, the immunosensor preparation method of a kind of current mode immunosensor rapid detector for pesticide residue of the present invention is: (1) electrode adopts 2 μ L Graphene-many walls carbon-nanometer gold size-chitosan nano compounds to drip and is coated onto each working electrode surface.Preparation method is as follows for Graphene-many walls carbon-nanometer gold size-chitosan nano compound: 0.5g shitosan is dissolved in the acetum of 100mL 1.0%, under room temperature, stirs 3h, be made into 0.5%CS solution, room temperature lower magnetic force stirs 1h, and shitosan is dissolved completely; Take 4.0mg Graphene and 4.0mg multi-walled carbon nano-tubes and add in 10.0mL chitosan solution obtained above, at room temperature ultrasonic dispersion, until obtain stable black dispersion liquid (Graphene-many walls carbon-chitosan nano composite material); Again the nanometer gold size of the 10.0mL preparing is added in the above-mentioned solution having mixed, more at room temperature ultrasonic dispersion 12h, to dissolving completely, obtains Graphene-many walls carbon-nanometer gold size-chitosan nano compound.(2) fixing means of antibody on working electrode: dripping painting 2 μ L concentration at the working electrode surface of having modified is 100 μ g/mL albumin As, then rinses with the phosphate buffer of pH7.5, and nitrogen dries up; Dripping painting 2 μ L concentration is 10 μ g/mL pesticide antibodies, hatches 12h for 4 ℃; By standing 1h under room temperature in the BSA solution of the last immersion 2.5% of the above-mentioned electrode preparing, with nonspecific binding site on enclosed-electrode, to reduce nonspecific interference.Under room temperature, dry, immunosensor completes, and is kept under 4 ℃ of conditions standby.
Described a kind of current mode immunosensor rapid detector for pesticide residue, is characterized in that: three-electrode system circuit is by three electrode immunosensor sensors, three electrode detection circuit, and potentiostatic circuit forms; Three electrode immunosensors gather current signal and are transmitted to input and disposal system circuit by three electrode detection circuit.Potentiostatic circuit provides the burning voltage of 500mV to reaction solution environment.
Input and disposal system circuit have I/V conversion, amplify, and filter function, includes A/D conversion chip and convert the analog signals through enzyme sensor collection after I/V conversion to digital quantity signal and send into single-chip microcomputer and carry out routine processes.The singlechip microcontroller of input and disposal system is STC89C52.The trace routine of single-chip microcomputer comprises data-printing and storage, time showing, and operation instruction and the dialog box to important step prompting, help user to grasp fast the using method of instrument and carry out data-printing and convenient storage carries out inspection and the processing of data later.Input and disposal system circuit adopt TLC272CP chip multichannel discharge circuit to have I/V conversion, amplify, and filtering also provides the function of 500mv constant voltage to three electrode reaction environment.
Show and print storage system circuit by display circuit, print circuit and memory circuitry form.Display controls for showing data the interface of controlling as user by operation push-button.Printing storage system circuit is connected and carries out data-printing and storage with mini-printer and storer respectively.Display screen has adopted the general display screen of 3.5 cun, and mini-printer adopts micro embedded printer, and its size and power consumption are satisfied to be convenient for carrying and portable power consumption requirement.
For reaching above object, take following technical scheme to realize: electric power system electric routing switch power supply and voltage conversion circuit form, Switching Power Supply converts alternating current to direct current and to each circuit system and mini-printer, powers respectively through voltage conversion circuit.Switching Power Supply is+12V, 1A ,-12V, 1A ,+5V ，5A tri-tunnels outputs.Power supply is connected with the Switching Power Supply in electric power system.
accompanying drawing explanation
Fig. 1 is the structured flowchart of a kind of current mode immunosensor of the present invention rapid detector for pesticide residue,
Fig. 2 is the present invention's three electrodes and potentiostatic circuit and A/D conversion treatment circuit in early stage.
Embodiment
First, structural representation block diagram of the present invention is illustrated; Secondly, input and included potentiostatic circuit, the A/D conversion treatment circuit in early stage of disposal system are described in detail; Finally, the application software of whole system and testing process are illustrated.
Fig. 1 is the structured flowchart of a kind of current mode immunosensor of the present invention rapid detector for pesticide residue.As shown in Figure 1, the present invention is by three-electrode system, and input and disposal system, show and print storage system, electric power system forms.Three-electrode system gathers the low current signal in reaction solution by immunosensor; The low current signal that input and disposal system collect three electrodes carries out I/V conversion, amplifies, and filtering, A/D conversion the most at last digital quantity signal is sent into single-chip microcomputer and is carried out routine processes; Show and print storage system by parameter displays such as residual pesticide concentration, inhibition degree out, and data being stored so that user checks in time and carries out data analysis.Detector of the present invention includes mini-printer, data can be carried out to corresponding printout.The single-chip microcomputer of input and disposal system inside carries out programming Control to showing with the unification of printing storage system.Electric power system converts alternating current to three road direct currents for each circuit system and mini-printer power supply.Described microcontroller adopts the STC89C52RC+ microcontroller that kernel is MCS-51, loads trace routine on it.
In the present invention, the potentiostatic circuit in testing circuit provides stable operating voltage, the embodiment that Fig. 2 is potentiostat circuit for three-electrode system current/voltage transforms testing circuit, by a two-way operational amplifier U10 TLC272CP and R44, R46, R4, C35, C16, R48 forms.The pin 6 of amplifier B is connected with 7 and is connected with 3 pin of A, 2 pin of A are connected and have connected C16 with the working electrode of three-electrode system, strengthen antijamming capability, R48 be as feedback resistance on amplifier A, the external potentiometer R46 of 5 pins of B, by positive 5V, supply electric excitation, R44, R46, R4 supplies with 500mv voltage to forming potentiostatic circuit with 5 pins of B by voltage dividing potential drop, initial voltage be 500mv. amplifier A and connect+5V of pin 8 power supply, connect-5V of pin 4, the amplifier B pin 7 of U9 TLC272CP is directly connected as voltage follower with pin 6, ADE pin 3 is connected with the pin 5 of B, common ground, play jamproof effect.The pin 2 of A is directly connected with the contrast electrode of three-electrode system, pin 1 is directly connected with the auxiliary electrode of three-electrode system, between connect electric capacity 14, object has been the effect of buffer circuit, can improve load-carrying ability simultaneously, therefore U9 and U10 have formed the three-electrode system of biology sensor jointly, and test side used, by drawing here, gathers and the low current signal that detects electrochemical reaction generation.
The immunosensor preparation method of a kind of current mode immunosensor rapid detector for pesticide residue of the present invention is: (1) electrode adopts 2 μ L Graphene-many walls carbon-nanometer gold size-chitosan nano compounds to drip and is coated onto each working electrode surface.Preparation method is as follows for Graphene-many walls carbon-nanometer gold size-chitosan nano compound: 0.5g shitosan is dissolved in the acetum of 100mL 1.0%, under room temperature, stirs 3h, be made into 0.5%CS solution, room temperature lower magnetic force stirs 1h, and shitosan is dissolved completely; Take 4.0mg Graphene and 4.0mg multi-walled carbon nano-tubes and add in 10.0mL chitosan solution obtained above, at room temperature ultrasonic dispersion, until obtain stable black dispersion liquid (Graphene-many walls carbon-chitosan nano composite material); Again the nanometer gold size of the 10.0mL preparing is added in the above-mentioned solution having mixed, more at room temperature ultrasonic dispersion 12h, to dissolving completely, obtains Graphene-many walls carbon-nanometer gold size-chitosan nano compound.(2) fixing means of antibody on working electrode: dripping painting 2 μ L concentration at the working electrode surface of having modified is 100 μ g/mL albumin As, then rinses with the phosphate buffer of pH7.5, and nitrogen dries up; Dripping painting 2 μ L concentration is 10 μ g/mL pesticide antibodies, hatches 12h for 4 ℃; By standing 1h under room temperature in the BSA solution of the last immersion 2.5% of the above-mentioned electrode preparing, with nonspecific binding site on enclosed-electrode, under room temperature, dry, immunosensor completes, and is kept under 4 ℃ of conditions standby.
A kind of sample treatment of current mode immunosensor rapid detector for pesticide residue is as follows: choose representational vegetables and fruit sample, with moist rag by the earth wiped clean of vegetable surface, be chopped into the square fragment of 2cm left and right, sample thief 2g, puts into extraction flask, adds 10ml phosphate buffer, put into ultrasonic extraction apparatus, ultrasonic extraction 3 minutes, pours out the standing 2min of extract, standby.
Testing process of the present invention is: first, the immunosensor preparing is put into not containing liquid at the bottom of the test of agricultural chemicals to the electric current I while recording not containing agricultural chemicals
1, electric current before suppressing (default value that before suppressing, electric current is set in also can employing system); Afterwards immunosensor is removed, with after distilled water flushing, immunosensor working electrode is put into solution to be detected and hatch 10 minutes, again immunosensor is put into test end liquid, record the electric current I after inhibition
2; Finally by the trace routine of moving on microcontroller, automatically calculate inhibiting rate (I%), computing formula is as follows:
I%
=( I
1- I
2)/ I
1 ×100%
If conventionally detect in solution and contain agricultural chemicals, because producing immune complex, antibody and agricultural chemicals generation immune response hindered the electronics transfer between test fluid and electrode, electric current I
2be less than I
1.I
1with I
2difference more inhibiting rate is larger, the concentration that shows to detect solution Pesticide Residues is higher; I
1with I
2the less inhibiting rate of difference less, the concentration that shows to detect solution Pesticide Residues is lower.
Think at present when inhibiting rate be greater than, while equaling 50%, residues of pesticides exceed standard; When inhibiting rate is less than 50%, residues of pesticides do not exceed standard.
Microcontroller is made qualitative conclusion according to the inhibiting rate obtaining, and result is delivered to display screen and show, and by end product printout, data is stored later the searching and studying for related personnel simultaneously in the lump.
The trace routine of moving on microcontroller of the present invention also comprises screen redirect demonstration and dialog box, print prompt to important step prompting, can help user to grasp fast the using method of instrument.
The embodiment of trace routine: first, immunosensor is put into substrate solution and detect preparation.Then, press instrument switch key, enter homepage after system initialization, display screen side has test button, reset and three of printings to broadcast mill button.Click for the first time test button, start to detect and show reading I
1; After clicking detection for the second time, observe prompting, show result I
2; Click and print, print result is also preserved.Opening power key, screen display " START detection ", zero clearing.Crocodile clip is stepped up respectively to three columnar electrodes that three-electrode system is corresponding, Pen-down detection key in the phosphate buffered solution of preparing before the immunosensor of sessile antibody is immersed in, screen display starts to detect, after about 1min, baseline reaches maintenance level, after about 30s, obtain first complete current data, be designated as I
1.Testing sample test: immunosensor is put into the sample solution having extracted, about 10min, is immersed in quantification of phosphate buffer solution afterwards again, presses measurement key, clicks detection key and measures for the second time, shows second current data I simultaneously
2.By two current data, automatically calculate the maximum inhibition of this sample liquid to immobilised enzymes.Select suitable relation curve to calculate the target pesticide concentration of extract, and be converted to the content of target agricultural chemicals in sample, the report of the last measurement result of generation automatically.Press printing key, the data that detect voltage, agricultural chemicals inhibiting rate and Pesticides Testing concentration before and after being printed on for twice are printed to output.
A kind of current mode immunosensor rapid detector for pesticide residue of the present invention, operating procedure is simple, detection time is shorter, can realize the qualitative, quantitative of residues of pesticides measures, have highly sensitive, good stability, high repeatability and other advantages, meet China's Fast Determination of Pesticide Residue technical development and internationalization requirement.
Claims (5)
1. a current mode immunosensor rapid detector for pesticide residue, is characterized in that: this detector is by immunosensor, input and treatment circuit, show and print memory circuit, constant potential feed circuit, operation push-button, data-carrier store, mini-printer, electric power generating composition; The current signal that immunosensor produces is transmitted to input and disposal system circuit by testing circuit; Potentiostatic circuit is to the stable operating voltage that 500mV is provided between working electrode and contrast electrode; Described input and treatment circuit have I/V conversion, amplify, and filter function, includes A/D conversion chip and convert the analog signals of enzyme sensor collection to digital quantity signal and send into single-chip microcomputer and carry out routine processes; Described immunosensor is used after nanometer-material-modified working electrode, and specific pesticide antibody is fixed to electrode surface, can in the measurement sample liquid of specificity, whether contain certain agricultural chemicals.
2. a kind of current mode immunosensor rapid detector for pesticide residue as claimed in claim 1, is characterized in that described input and the singlechip microcontroller of disposal system are STC89C52.
3. a kind of current mode immunosensor rapid detector for pesticide residue as claimed in claim 1, what it is characterized in that this detector use is immunosensor, at immunosensor working electrode surface, fixed specific pesticide antibody, therefore this detector can realize in the working sample of specificity whether containing certain agricultural chemicals, realizes the qualitative, quantitative of sample and measures.
4. a kind of current mode immunosensor rapid detector for pesticide residue as claimed in claim 1, it is characterized in that described input and disposal system circuit adopt TLC272CP chip multichannel discharge circuit to have I/V conversion, amplify, filtering also provides the function of 500mv constant voltage to three electrode reaction environment.
5. the current mode immunosensor rapid detector for pesticide residue described in, it is characterized in that adopting Graphene-many walls carbon-nanometer gold size-chitosan nano compound nano-complex modified electrode, and with a-protein, pesticide antibody is fixed on to the working electrode surface of having modified, finally with bovine serum albumin, make sealer with the non-specific site on enclosed-electrode surface, to reduce nonspecific interference.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105004859A (en) * | 2015-07-14 | 2015-10-28 | 山东理工大学 | Preparation method and use of Pd/V2O5/MWCNTs-based intestinal cancer tumor marker immunosensor |
| CN105424770A (en) * | 2015-11-10 | 2016-03-23 | 中国科学院电子学研究所 | Electrochemical detector for detecting direct electrochemical immunity sensor |
| CN106066356A (en) * | 2016-03-07 | 2016-11-02 | 天津科技大学 | The intelligent portable Graphene of a kind of EC of detection prints electrode detector |
| CN110470721A (en) * | 2019-07-12 | 2019-11-19 | 佛山职业技术学院 | A kind of acrylamide electrochemical fast detecting method |
| CN112630287A (en) * | 2020-12-14 | 2021-04-09 | 山东理工大学 | Multi-channel organophosphorus pesticide rapid detector and detection method based on Internet of things |
| CN114660151A (en) * | 2022-05-06 | 2022-06-24 | 江苏大学 | Preparation method and application of double-electric-field driving sensor |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1144336A (en) * | 1995-08-25 | 1997-03-05 | 福州大学 | Medical immunoassay device |
| CN1967228A (en) * | 2005-11-18 | 2007-05-23 | 上海环轩信息技术有限公司 | Method for making immune sensing electrode for detection of residual chloromycetin |
| CN101149384A (en) * | 2007-09-30 | 2008-03-26 | 浙江工商大学 | Device for detecting avian influenza virus antigen and detection method |
| EP1912062A1 (en) * | 2006-10-13 | 2008-04-16 | Sabanci Universitesi | A biosensor and chemical sensor implementation using RF and microwave device, circuits and systems |
| CN102103122A (en) * | 2011-01-26 | 2011-06-22 | 山东理工大学 | Rapid detector and detection method for electrochemical pesticide residue |
| CN102226779A (en) * | 2011-03-28 | 2011-10-26 | 中国人民解放军第三军医大学第三附属医院 | Electrochemical immunodetection method |
| CN102608188A (en) * | 2012-03-30 | 2012-07-25 | 山东理工大学 | Preparation method of current mode immunosensor used for detecting pesticide residue |
| CN102914583A (en) * | 2012-05-07 | 2013-02-06 | 赵朝辉 | Portable electrochemistry biochip detector system |
| JP2013228280A (en) * | 2012-04-26 | 2013-11-07 | Hitachi Ltd | Magnetic immunity inspection method and inspection device using ac magnetic field |
| CN103592437A (en) * | 2013-11-11 | 2014-02-19 | 山东理工大学 | Immunosensor based on modification of graphene-multiwalled carbon-nanogold size-chitosan |
-
2013
- 2013-11-19 CN CN201310576569.8A patent/CN103558374B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1144336A (en) * | 1995-08-25 | 1997-03-05 | 福州大学 | Medical immunoassay device |
| CN1967228A (en) * | 2005-11-18 | 2007-05-23 | 上海环轩信息技术有限公司 | Method for making immune sensing electrode for detection of residual chloromycetin |
| EP1912062A1 (en) * | 2006-10-13 | 2008-04-16 | Sabanci Universitesi | A biosensor and chemical sensor implementation using RF and microwave device, circuits and systems |
| CN101149384A (en) * | 2007-09-30 | 2008-03-26 | 浙江工商大学 | Device for detecting avian influenza virus antigen and detection method |
| CN102103122A (en) * | 2011-01-26 | 2011-06-22 | 山东理工大学 | Rapid detector and detection method for electrochemical pesticide residue |
| CN102226779A (en) * | 2011-03-28 | 2011-10-26 | 中国人民解放军第三军医大学第三附属医院 | Electrochemical immunodetection method |
| CN102608188A (en) * | 2012-03-30 | 2012-07-25 | 山东理工大学 | Preparation method of current mode immunosensor used for detecting pesticide residue |
| JP2013228280A (en) * | 2012-04-26 | 2013-11-07 | Hitachi Ltd | Magnetic immunity inspection method and inspection device using ac magnetic field |
| CN102914583A (en) * | 2012-05-07 | 2013-02-06 | 赵朝辉 | Portable electrochemistry biochip detector system |
| CN103592437A (en) * | 2013-11-11 | 2014-02-19 | 山东理工大学 | Immunosensor based on modification of graphene-multiwalled carbon-nanogold size-chitosan |
Non-Patent Citations (1)
| Title |
|---|
| 贾翠娟 等: "基于石墨烯/壳聚糖/碳纳米管复合膜的新型电流型甲胎蛋白传感器", 《化学通报》, vol. 75, no. 11, 18 November 2012 (2012-11-18) * |
Cited By (9)
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| CN105004859A (en) * | 2015-07-14 | 2015-10-28 | 山东理工大学 | Preparation method and use of Pd/V2O5/MWCNTs-based intestinal cancer tumor marker immunosensor |
| CN105004859B (en) * | 2015-07-14 | 2016-06-29 | 山东理工大学 | A kind of based on Pd/V2O5The preparation method of the intestinal cancer tumor markers immunosensor of/MWCNTs and application |
| CN105424770A (en) * | 2015-11-10 | 2016-03-23 | 中国科学院电子学研究所 | Electrochemical detector for detecting direct electrochemical immunity sensor |
| CN105424770B (en) * | 2015-11-10 | 2018-11-09 | 中国科学院电子学研究所 | A kind of Electrochemical detector for the detection of Direct Electrochemistry immunosensor |
| CN106066356A (en) * | 2016-03-07 | 2016-11-02 | 天津科技大学 | The intelligent portable Graphene of a kind of EC of detection prints electrode detector |
| CN110470721A (en) * | 2019-07-12 | 2019-11-19 | 佛山职业技术学院 | A kind of acrylamide electrochemical fast detecting method |
| CN112630287A (en) * | 2020-12-14 | 2021-04-09 | 山东理工大学 | Multi-channel organophosphorus pesticide rapid detector and detection method based on Internet of things |
| CN114660151A (en) * | 2022-05-06 | 2022-06-24 | 江苏大学 | Preparation method and application of double-electric-field driving sensor |
| CN114660151B (en) * | 2022-05-06 | 2023-01-17 | 江苏大学 | Preparation method and application of double-electric-field driving sensor |
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