CN103555627B - A kind of feeding bacillus living formulation preserves carrier and preparation method and application - Google Patents
A kind of feeding bacillus living formulation preserves carrier and preparation method and application Download PDFInfo
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- CN103555627B CN103555627B CN201310535870.4A CN201310535870A CN103555627B CN 103555627 B CN103555627 B CN 103555627B CN 201310535870 A CN201310535870 A CN 201310535870A CN 103555627 B CN103555627 B CN 103555627B
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Abstract
The present invention discloses a kind of feeding bacillus living formulation and preserves carrier and preparation method thereof and application.Feeding bacillus living formulation provided by the invention preserves carrier, comprises following component by mass percentage: medical stone 50% ~ 70%, talcum powder 10% ~ 40%, white carbon black 5% ~ 10%, calcium carbonate 1% ~ 2%, phenylalanine 0.1% ~ 0.3%.The feeding bacillus living formulation of the present invention is preserved carrier and is had good dispersity, not easily moisture absorption caking, the feature of high adsorption capacity; Meanwhile, preserve carrier raw material sources used extensive, cost is low, and production operation is convenient, and genus bacillus can be made to preserve for a long time, and stable content.
Description
Technical field
The invention belongs to feeding micro-ecological preparation field, be specifically related to a kind of feeding bacillus living formulation and preserve carrier and preparation method and application.
Background technology
Along with the development of science and technology, feeding micro-ecological preparation obtains rapidly universal, has the advantages such as nontoxic, harmless, noresidue, so become the novel fodder additive of substitute antibiotics because of it.Feeding micro-ecological preparation arises at the historic moment based on the safety issue of feeding antibiotic just, it can not only play restraining effect to pathogenic bacterium, the nontoxic and promotion growth of animals or poultry to livestock product, and livestock product meat flavor can be improved, being conducive to ecotope and the export trade, is the best solution of following green feed.
The production of current feeding micro-ecological preparation adopts liquid submerged fermentation method more, through spray drying technology, makes high-concentration bacterial powder, the probiotics that this fermentation process is produced have purity high, pollute little, the active advantage such as strong.But easily the moisture absorption is lumpd on preserving, viable bacteria content lowering speed is fast, brings a difficult problem to production and application, is therefore badly in need of developing a kind of preservation carrier can preserving this type of feeding micro-ecological preparation for a long time, for fodder production and livestock industry bring benefit.
Summary of the invention
In order to overcome the shortcoming of prior art with not enough, primary and foremost purpose of the present invention is that providing one to be applicable to the spray-dired feeding bacillus living formulation of liquid submerged fermentation preserves carrier.
Another object of the present invention is to provide described feeding bacillus living formulation to preserve the preparation method of carrier, and the method technical process is simple, and operation steps is few, easy to implement.
Another object of the present invention is to provide described feeding bacillus living formulation to preserve the application of carrier.
Object of the present invention is achieved through the following technical solutions: a kind of feeding bacillus living formulation preserves carrier, comprise following component by mass percentage: medical stone 50% ~ 70%, talcum powder 10% ~ 40%, white carbon black 5% ~ 10%, calcium carbonate 1% ~ 2%, phenylalanine 0.1% ~ 0.3%.
In order to better realize the present invention,
A kind of feeding bacillus living formulation preserves carrier, preferably comprises following component by mass percentage: medical stone 50% ~ 70%, talcum powder 20% ~ 40%, white carbon black 7.8% ~ 8.7%, calcium carbonate 1% ~ 2%, phenylalanine 0.1% ~ 0.3%;
The biodiversity content of described medical stone, talcum powder, white carbon black, calcium carbonate and phenylalanine is preferably all below 10%;
A kind of feeding bacillus living formulation preserves carrier, preferably comprises following component by mass percentage: medical stone 70%, talcum powder 20%, white carbon black 8.7%, calcium carbonate 1%, phenylalanine 0.3%; The biodiversity content of described each component is preferably: medical stone 3%, talcum powder 5%, white carbon black 5%, calcium carbonate 7%, phenylalanine 5%.
Described feeding bacillus living formulation preserves the preparation method of carrier, comprises following concrete steps:
(1) medical stone, talcum powder, white carbon black, calcium carbonate and phenylalanine are pulverized respectively, sieve;
(2) take medical stone, talcum powder, white carbon black, calcium carbonate and the phenylalanine after sieving in step (1) according to the above ratio, mix step by step from low to high by its addition, stir, make feeding bacillus living formulation and preserve carrier;
Pulverizing described in step (1) is preferably strengthened superfine mill with T130X and is pulverized;
Preferably use 200 mesh sieve that sieves described in step (1);
Stir preferably with vertical KX-500 type electric metering powder stirrer described in step (2), under 450 ~ 500r/min, stir 10 ~ 20min.
Described feeding bacillus living formulation is preserved carrier and is being preserved the application in feeding bacillus living formulation, preferably comprise following steps: by feeding bacillus living formulation and preservation carrier 1:(0.33 ~ 9 in mass ratio) composite, being prepared into viable bacteria content is 2.0 × 10
10~ 1.5 × 10
11the feeding micro-ecological preparation of cfu/g.
Described feeding bacillus living formulation be preferably liquid submerged fermentation spray-dried after feeding bacillus living formulation;
Described feeding bacillus living formulation is preferably at least one in live bacillus subtilis preparation, bacillus cicheniformis, coagulated bacillus living formulation, bacillus cereus active bacteria formulation and bacillus natto active bacteria formulation;
The composite amount of described preservation carrier is suitable with the active bacteria formulation proportion that will preserve, and the proportioning of preserving carrier and active bacteria formulation is equipped with according to needs of production.
The present invention compared with prior art has following advantages and effect:
(1) described preservation support material medical stone, talcum powder, white carbon black, calcium carbonate are inorganic raw material, and are inert raw material, and it can make thalline be in dormant state, keeps activity stabilized; And these raw materials contain major element needed for many growth of animal and trace element.Wherein medical stone good palatability, can prevent from raising imbalance, alleviate excremental smell, and contribute to the stalwartness of domestic animal.Phenylalanine is conducive to the formation of gemma, and it is also one of necessary amino acid of animal simultaneously, can improve the immunizing power of animal.
(2) the feeding bacillus living formulation of the present invention preserves carrier, can be good at adsorbing thalline, and product long-term storage can not be lumpd in layering, and bacterium is uniformly dispersed, active lasting; When the feeding bacillus living formulation that preservation liquid submerged fermentation is spray-dried, good dispersity, nonhygroscopic, do not lump; Its high adsorption capacity, can active adsorption probiotics.
(3) the feeding bacillus living formulation of the present invention is preserved carrier and is improve probiotics stability, substantially prolongs its quality guaranteed period.Solve the problem such as the easy moisture absorption of probiotics after existing spraying dry, caking, inactivation, not easily long-term preservations, and can damage from external environment by available protecting probiotics, ensure its activity.Also improve probiotics viable bacteria recall rate simultaneously, reduce examination criteria error.Ensure that the application that probiotics is stable for a long time in fodder production.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
Embodiment 1
A kind of feeding bacillus living formulation preserves carrier, and the mass percent shared by each composition is respectively: medical stone 50%, talcum powder 40%, white carbon black 8%, calcium carbonate 1.9%, phenylalanine 0.1%.Its moisture content of each composition is respectively: medical stone 3%, talcum powder 5%, white carbon black 5%, calcium carbonate 7%, phenylalanine 5%.
Described feeding bacillus living formulation preserves the preparation method of carrier, comprises the steps:
(1) medical stone, talcum powder, white carbon black, calcium carbonate and phenylalanine are all pulverized, cross 200 mesh sieves, make its fineness reach 200 orders;
(2), after crossing 200 mesh sieves, take each component respectively by above-mentioned formula, each component mixes from low to high step by step by its addition, with vertical KX-500 type electric metering powder stirrer, under 480r/min, stir 15min, stir, make feeding bacillus living formulation and preserve carrier.
Embodiment 2
A kind of feeding bacillus living formulation preserves carrier, and the mass percent shared by each composition is respectively: medical stone 60%, talcum powder 30%, white carbon black 7.8%, calcium carbonate 2%, phenylalanine 0.2%.Its moisture content of each composition is respectively: medical stone 3%, talcum powder 5%, white carbon black 5%, calcium carbonate 7%, phenylalanine 5%.
Described feeding bacillus living formulation preserves the preparation method of carrier, comprises the steps:
(1) medical stone, talcum powder, white carbon black, calcium carbonate and phenylalanine are all pulverized, cross 200 mesh sieves, make its fineness reach 200 orders;
(2), after crossing 200 mesh sieves, take each component respectively by above-mentioned formula, each component mixes from low to high step by step by its addition, with vertical KX-500 type electric metering powder stirrer, under 450r/min, stir 20min, stir, make feeding bacillus living formulation and preserve carrier.
Embodiment 3
A kind of feeding bacillus living formulation preserves carrier, and the mass percent shared by each composition is respectively: medical stone 70%, talcum powder 20%, white carbon black 8.7%, calcium carbonate 1%, phenylalanine 0.3%.Its moisture content of each composition is respectively: medical stone 3%, talcum powder 5%, white carbon black 5%, calcium carbonate 7%, phenylalanine 5%.
Described feeding bacillus living formulation preserves the preparation method of carrier, comprises the steps:
(1) medical stone, talcum powder, white carbon black, calcium carbonate and phenylalanine are all pulverized, cross 200 mesh sieves, make its fineness reach 200 orders;
(2), after crossing 200 mesh sieves, take each component respectively by above-mentioned formula, each component mixes from low to high step by step by its addition, with vertical KX-500 type electric metering powder stirrer, under 500r/min, stir 10min, stir, make feeding bacillus living formulation and preserve carrier.
Effect example
Preserve carrier and preparation method according to the feeding bacillus living formulation of the present invention, this is preserved carrier be applied to respectively liquid submerged fermentation spray-dried after subtilis, Bacillus licheniformis, Bacillus coagulans, bacillus cereus and bacillus natto active bacteria formulation (above active bacteria formulation is all bought from Bo Shiao bio tech ltd, the Sanmenxia Gorge).According to need of production, each bacteria containing amount is 2.0 × 10
111:0.33,1:1,1:9 are composite in mass ratio respectively with preserving carrier for the bacillus living formulation of cfu/g, and being prepared into viable bacteria content is 1.5 × 10
11cfu/g, 1.0 × 10
11cfu/g, 2.0 × 10
10the feeding micro-ecological preparation of cfu/g.Routine observation sampling and measuring viable bacteria content.Test-results shows, use the present invention to preserve the bacillus living formulation good dispersity of carrier, nonhygroscopic, do not lump, long quality-guarantee period, activity stabilized, room temperature preservation 12 months its each sporeformer viable bacteria rate still can reach more than 90%.As table 1,2, shown in 3.
The viable bacteria rate (%) of the feeding micro-ecological preparation after table 1 bacillus living formulation and embodiment 1 proportioning
The viable bacteria rate (%) of the feeding micro-ecological preparation after table 2 bacillus living formulation and embodiment 2 proportioning
The viable bacteria rate (%) of the feeding micro-ecological preparation after table 3 bacillus living formulation and embodiment 3 proportioning
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from spirit of the present invention and principle, substitute, combine, simplify; all should be the substitute mode of equivalence, be included within protection scope of the present invention.
Claims (10)
1. a feeding bacillus living formulation preserves carrier, it is characterized in that comprising following component by mass percentage: medical stone 50% ~ 70%, talcum powder 20% ~ 40%, white carbon black 7.8% ~ 8.7%, calcium carbonate 1% ~ 2%, phenylalanine 0.1% ~ 0.3%;
The biodiversity content of described medical stone, talcum powder, white carbon black, calcium carbonate and phenylalanine is all below 10%.
2. feeding bacillus living formulation according to claim 1 preserves carrier, it is characterized in that comprising following component by mass percentage: medical stone 70%, talcum powder 20%, white carbon black 8.7%, calcium carbonate 1%, phenylalanine 0.3%; The biodiversity content of described each component is: medical stone 3%, talcum powder 5%, white carbon black 5%, calcium carbonate 7%, phenylalanine 5%.
3. the feeding bacillus living formulation according to any one of claim 1 ~ 2 preserves the preparation method of carrier, it is characterized in that comprising following concrete steps:
(1) medical stone, talcum powder, white carbon black, calcium carbonate and phenylalanine are pulverized respectively, sieve;
(2) medical stone, talcum powder, white carbon black, calcium carbonate and the phenylalanine after sieving in step (1) is taken according to the above ratio, mix step by step from low to high by its addition, stir, make feeding bacillus living formulation and preserve carrier.
4. feeding bacillus living formulation according to claim 3 preserves the preparation method of carrier, it is characterized in that: the pulverizing described in step (1) strengthens superfine mill with T130X to pulverize;
Sieving as using 200 mesh sieve described in step (1).
5. feeding bacillus living formulation according to claim 3 preserves the preparation method of carrier, it is characterized in that: stirring described in step (2) is with vertical KX-500 type electric metering powder stirrer, under 450 ~ 500r/min, stir 10 ~ 20min.
6. the feeding bacillus living formulation described in any one of claim 1 ~ 2 is preserved carrier and is being preserved the application in feeding bacillus living formulation.
7. feeding bacillus living formulation according to claim 6 is preserved carrier and is being preserved the application in feeding bacillus living formulation, it is characterized in that comprising following steps: by feeding bacillus living formulation and preservation carrier 1:(0.33 ~ 9 in mass ratio) composite, being prepared into viable bacteria content is 2.0 × 10
10~ 1.5 × 10
11the feeding micro-ecological preparation of cfu/g.
8. the feeding bacillus living formulation according to claim 6 or 7 is preserved carrier and is being preserved the application in feeding bacillus living formulation, it is characterized in that: described feeding bacillus living formulation be liquid submerged fermentation spray-dried after feeding bacillus living formulation.
9. the feeding bacillus living formulation according to claim 6 or 7 preserves carrier preserving the application in feeding bacillus living formulation, it is characterized in that: described feeding bacillus living formulation is at least one in live bacillus subtilis preparation, bacillus cicheniformis, coagulated bacillus living formulation, bacillus cereus active bacteria formulation and bacillus natto active bacteria formulation.
10. feeding bacillus living formulation according to claim 8 preserves carrier preserving the application in feeding bacillus living formulation, it is characterized in that: described feeding bacillus living formulation is at least one in live bacillus subtilis preparation, bacillus cicheniformis, coagulated bacillus living formulation, bacillus cereus active bacteria formulation and bacillus natto active bacteria formulation.
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| CN201310535870.4A CN103555627B (en) | 2013-11-01 | 2013-11-01 | A kind of feeding bacillus living formulation preserves carrier and preparation method and application |
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| CN103555627B true CN103555627B (en) | 2016-01-06 |
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Citations (4)
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|---|---|---|---|---|
| CN1088563A (en) * | 1992-12-25 | 1994-06-29 | 郭城 | The production method of efficient biological organic complex fertilizer |
| CN102178110A (en) * | 2011-05-12 | 2011-09-14 | 比利美英伟营养饲料（深圳）有限公司 | Premix feed for improving reproductive performance of boar |
| CN102994038A (en) * | 2011-09-13 | 2013-03-27 | 孟凡丽 | Preparation of negative ion additive |
| CN103131655A (en) * | 2013-03-06 | 2013-06-05 | 江苏苏滨生物农化有限公司 | Bacillus amyloliquefaciens K-8 and bactericide thereof |
-
2013
- 2013-11-01 CN CN201310535870.4A patent/CN103555627B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1088563A (en) * | 1992-12-25 | 1994-06-29 | 郭城 | The production method of efficient biological organic complex fertilizer |
| CN102178110A (en) * | 2011-05-12 | 2011-09-14 | 比利美英伟营养饲料（深圳）有限公司 | Premix feed for improving reproductive performance of boar |
| CN102994038A (en) * | 2011-09-13 | 2013-03-27 | 孟凡丽 | Preparation of negative ion additive |
| CN103131655A (en) * | 2013-03-06 | 2013-06-05 | 江苏苏滨生物农化有限公司 | Bacillus amyloliquefaciens K-8 and bactericide thereof |
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