CN103555626B - Serratia marcescens S2 and application of serratia marcescens S2 in degradation of dibenzothiophene - Google Patents
Serratia marcescens S2 and application of serratia marcescens S2 in degradation of dibenzothiophene Download PDFInfo
- Publication number
- CN103555626B CN103555626B CN201310525022.5A CN201310525022A CN103555626B CN 103555626 B CN103555626 B CN 103555626B CN 201310525022 A CN201310525022 A CN 201310525022A CN 103555626 B CN103555626 B CN 103555626B
- Authority
- CN
- China
- Prior art keywords
- dibenzothiophene
- serratia marcescens
- degradation
- application
- bacterial strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses serratia marcescens S2 capable of effectively degrading dibenzothiophene and application of the serratia marcescens S2 in degradation of dibenzothiophene. The dibenzothiophene degrading bacteria is the serratia marcescens S2, the preservation number is CGMCC N0.3654. The serratia marcescens S2 with preservation number (CGMCC N0.3654) is used for degrading dibenzothiophene. The bacterial strain has high degrading speed.
Description
Technical field
The invention belongs to the Application Areas of microorganism in environmental pollutant degraded, relate to a strain dibenzothiophene degradation bacteria and the application in environmental pollution is controlled thereof.
Background technology
Except containing organosulfur heterogeneous ring compound in coke-oven waste water as dibenzothiophene etc., dibenzothiophene is the main component in crude oil sulphur impurity, occupies the 40%-70% of total sulfur in some oil.Oil, petroleum chemicals and coking are produced in production, transportation and use procedure can produce a large amount of pollution, as the pollution of soil and underground water and a large amount of waste water, dibenzothiophene is significant for the processing of soil organisms reparation and petrifaction sewage, coke-oven waste water as the degraded of one of a kind of main pollutent.
Biological treating be microorganism utilize pollutent as carbon source or other nutrition sources as nitrogenous source, sulphur source etc., decomposed to utilize and be converted into carbonic acid gas, water and inorganic mineral etc.Biological treating/processing is because advantages such as low, simple to operate, the mild condition of cost and non-secondary pollutions; in the process of the processing of soil organisms reparation and petrifaction sewage, coke-oven waste water, there is absolute advantage; screening, acquisition have high-effective microorganism bacterial strain dibenzothiophene to fast degradation ability; can be added in soil and sewage disposal to be repaired; significantly improve the effect of contaminant degradation, this is ecological significant to protecting.
In general biological degradation bacterium requires to have the advantages such as, fast growth low to growing environment requirement, degradation speed be fast.A strain dibenzothiophene degradation bacteria provided by the invention has above-mentioned advantage.
Summary of the invention
The object of this invention is to provide bacterial strain and the application thereof of a strain degradable dibenzothiophene.
The present invention be take dibenzothiophene certain oil field sludge from China and is had the ability of fast degradation dibenzothiophene as serratia marcescens (Serratia marcescens) bacterial strain that the screening of unique sulphur source obtains.At dibenzothiophene, be that on the Selective solid culture medium in unique sulphur source, growth has pink bacterium colony, in about 30 ℃ growths.
This bacterial strain is serratia marcescens S2(Serratia marcescens S2), preserving number CGMCC No.3654.
Another object of the present invention is to provide the application method of this bacterial strain: degraded is containing dibenzothiophene pollutent.
Serratia marcescens S2(Serratia marcescens S2 provided by the invention), growth rapidly, easily become dominant strain, can be efficiently, fast degradation dibenzothiophene, be applicable to carrying out the processing of oil, coke-oven waste water and the reparation of oil-polluted soils and underground water.By detailed description below, the feature and advantage of these aspects of the present invention will be more clear.
In the present invention, unless specialized, term " containing dibenzothiophene pollutent " refers to the petrochemical industry that obviously contains dibenzothiophene, the coking three wastes or the oil-polluted soils that produce in commercial run, underground water etc.
Embodiment
(1) substratum
Basic salts solution (BSM): KH
2pO
4: 2.5g, NaHPO
412H
20:12.0g, MgCl
26H
2o:0.8g, NH
4cl:2.0g, CaCl
2: 0.07g, FeCl
36H
2o:0.1g, MnCl
44H
2o:0.4g, is dissolved in 1000ml deionized water, makes salts solution.
Enrichment, screening culture medium: in BSM solution, add glycerine 15.0g/L, dibenzothiophene 800mg/L.
Solid medium: add glycerine 15.0g/L in BSM solution, dibenzothiophene 800mg/L, adds 20g/L agar in solid medium, and moist heat sterilization is standby, condition change separately explains.
Dibenzothiophene artificial wastewater: add glycerine 5.0g/L in BSM solution, dibenzothiophene 800mg/L.
(2) screening of bacterial strain and separation and purification
From the soil of refinery, being placed in 30 ℃ of shaking tables activation of 250ml Erlenmeyer flask pipettes 10ml with transfer pipet after 2 days and adds enrichment medium, after shaking table is cultivated three days, 4000rpm is centrifugal 5 minutes, outwell supernatant liquor, mud bacterium is transferred to corresponding fresh enrichment medium in part bottom, so within every three days, repeat once totally 3 times.Then pipette appropriate domestication bacterium liquid and carry out dilution spread flat board, after cultivating in 30 ℃ of incubators, choose quantity many, grow fine, bacterium colony that outward appearance is different, the separation and purification of repeatedly ruling obtains purebred bacterial strain, after being cultivated, the inoculation slant medium of purifying in 4 ℃ of refrigerator preservations, every 3-4 month, transfers once.
(3) molecular biology identification of bacterial strain
Identification of strains adopts 16S rDNA to carry out, forward primer 5 '-AGAGTTTGATC (C/A) TGGCTCAG-3 ' (SEQ ID NO.2), reverse primer: 5 '-TACGG (C/T) TACCTTGTTACGACTT-3 ' (SEQ ID NO.3), PCR(ABI9700) condition: 94 ℃ of denaturation 2min, 94 ℃ of sex change 20s, 52 ℃ of annealing 10s, 72 extend 130s, 35 circulations, 72 extend 2min, and ABI3700DNA sequenator is used in order-checking.16S rDNA sequence is as shown in SEQ ID NO.1.NCBI BLAST16S rDNA sequence search result and serratia marcescens (Serratia marcescens) bacterial strain homology 98%, called after serratia marcescens S2(Serratia marcescens S2).Be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC), address on March 5th, 2010: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, preserving number CGMCC No.3654; Classification And Nomenclature is: Serratia marcescens.
(4) dibenzothiophene degraded
Dull and stereotyped cultivation: bacterial classification serratia marcescens S2(Serratia marcescens S2) inoculation solid medium is placed on 30 ℃ of constant incubators and cultivates 1-2 days.
Contaminant degradation: bacterial classification inoculation artificial wastewater, be placed in 30 ℃, in the shaking table of 200r/min, carry out utilizing equal-volume hexanaphthene extraction residue dibenzothiophene after shaking culture certain hour, for analyzing.
(5) dibenzothiophene test: use Agilent gas-chromatography (6890) to carry out, fid detector, 40 ℃/min of 50-300 ℃ of heat-up rate, chromatographic column HP-5 capillary chromatographic column.
Embodiment 1 bacterial classification serratia marcescens S2(Serratia marcescens S2) inoculation dibenzothiophene artificial wastewater, DBT concentration 800mg/L, is placed in 30 ℃, vibrates after 48 hours in the shaking table of 180r/min, and dibenzothiophene degradation rate reaches 83%.
Embodiment 2 bacterial classification serratia marcescens S2(Serratia marcescens S2) inoculation dibenzothiophene artificial wastewater, DBT concentration 400mg/L, is placed in 30 ℃, vibrates after 24 hours in the shaking table of 180r/min, and dibenzothiophene degradation rate reaches 66%.
Claims (4)
1. a strain serratia marcescens S2(
serratia marcescenss2), its preserving number is CGMCC No.3654.
2. serratia marcescens S2 as claimed in claim 1, is characterized in that its 16S rDNA sequence is as shown in SEQ ID NO.1.
3. the application of serratia marcescens S2 bacterial strain as claimed in claim 1 in degradation of dibenzothiophene.
4. application as claimed in claim 3, is characterized in that, serratia marcescens S2 inoculation is degraded to containing in the pollutent of dibenzothiophene.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310525022.5A CN103555626B (en) | 2013-10-30 | 2013-10-30 | Serratia marcescens S2 and application of serratia marcescens S2 in degradation of dibenzothiophene |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310525022.5A CN103555626B (en) | 2013-10-30 | 2013-10-30 | Serratia marcescens S2 and application of serratia marcescens S2 in degradation of dibenzothiophene |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103555626A CN103555626A (en) | 2014-02-05 |
| CN103555626B true CN103555626B (en) | 2014-12-10 |
Family
ID=50009992
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310525022.5A Expired - Fee Related CN103555626B (en) | 2013-10-30 | 2013-10-30 | Serratia marcescens S2 and application of serratia marcescens S2 in degradation of dibenzothiophene |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103555626B (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106497813B (en) * | 2015-09-07 | 2019-06-25 | 粮华生物科技（北京）有限公司 | Serratia marcescens and microbial inoculum and their applications in degrading polycyclic aromatic hydrocarbons |
| CN106399172B (en) * | 2016-09-19 | 2019-06-14 | 河南大学 | The Serratieae for lauryl sodium sulfate of degrading and its application |
| CN110468079A (en) * | 2019-08-28 | 2019-11-19 | 中国科学院上海高等研究院 | A kind of sludge degradation strain and its application |
| CN110804567B (en) * | 2019-11-14 | 2021-03-02 | 重庆理工大学 | Serratia marcescens capable of degrading chlorobenzene and application thereof |
| CN110804568B (en) * | 2019-11-14 | 2021-03-02 | 重庆理工大学 | Complex microbial inoculant and application thereof |
| CN111394284B (en) * | 2020-04-28 | 2021-10-15 | 河北萌帮水溶肥料股份有限公司 | Serratia marcescens MB21 and application thereof |
| CN111357770B (en) * | 2020-05-12 | 2020-08-28 | 广东省生物资源应用研究所 | Application of serratia marcescens in preparation of termite antifeedant |
| CN113800647A (en) * | 2020-10-16 | 2021-12-17 | 西安工程大学 | Application of serratia marcescens strain in decolorizing and degrading vat blue RSN anthraquinone dye |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101538605B (en) * | 2008-03-19 | 2011-09-14 | 中国科学院沈阳应用生态研究所 | Method for screening degradation bacteria strains by taking high-efficiency cyhalothrin as substrate |
| CN101851587B (en) * | 2010-06-08 | 2011-10-26 | 南京师范大学 | Fusarium solani and application thereof in degradation of dibenzothiophene |
| CN102337234A (en) * | 2011-07-29 | 2012-02-01 | 东南大学 | Serratia marcescens LTH-2 as well as screening method and application thereof |
| CN102358942B (en) * | 2011-09-23 | 2013-06-26 | 蚌埠市钰诚新材料科技有限公司 | Preparation method for biological component of biological compound degreaser |
-
2013
- 2013-10-30 CN CN201310525022.5A patent/CN103555626B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN103555626A (en) | 2014-02-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103555626B (en) | Serratia marcescens S2 and application of serratia marcescens S2 in degradation of dibenzothiophene | |
| CN101851587B (en) | Fusarium solani and application thereof in degradation of dibenzothiophene | |
| Al-Mailem et al. | Biostimulation of indigenous microorganisms for bioremediation of oily hypersaline microcosms from the Arabian Gulf Kuwaiti coasts | |
| Prakash et al. | Biodegradation potential of petroleum hydrocarbons by bacteria and mixed bacterial consortium isolated from contaminated sites | |
| Koutra et al. | Microalgal post-treatment of anaerobically digested agro-industrial wastes for nutrient removal and lipids production | |
| CN103555612B (en) | Light yellow mycobacterium and its application in degradation of oil components polycyclic aromatic hydrocarbons | |
| CN102925391B (en) | Pseudomonas aeruginosa and obtaining method and application thereof | |
| CN102242071B (en) | Application of Pichia guilliermondii 510-6jm in treatment of petroleum hydrocarbon contaminations | |
| CN106635908B (en) | Marine oil degrading bacterium, microbial inoculum and application thereof | |
| CN103981119A (en) | Petroleum efficient degrading bacterium and bacterium group both in oily sludge and application of two | |
| CN108130288A (en) | The application of Rhodococcus ruber and its degradable organic pollutant | |
| CN102776125A (en) | Construction method of moderate halophilic bacteria consortium capable of degrading high-salinity benzene series and application of moderate halophilic bacteria consortium | |
| Essa et al. | Potential cultivation of halophilic oleaginous microalgae on industrial wastewater | |
| CN100475950C (en) | Bacillus megaterium and use thereof | |
| CN104001712A (en) | Method for repairing petroleum-contaminated soil through combination of alkali grass and microbes | |
| CN105018392B (en) | One plant of long-chain alkane degrading bacterium and its application | |
| CN105670956A (en) | Medium thermophilic bacillus licheniformis strain and application thereof in treatment on high-temperature oil-bearing wastewater | |
| CN104789506A (en) | Thalassospira sp. capable of degrading polycyclic aromatic hydrocarbons under saline environment and application thereof | |
| CN103992976A (en) | Petroleum hydrocarbon degradation bacteria DL5-3 and application thereof | |
| CN107475144B (en) | Pandora and using method thereof | |
| Solomon et al. | Influence of biostimulation treatment using composted plant biomass on bacterial diversity of an aged petroleum contaminated soil as determined by culture-dependent and 16S rRNA gene PCR-DGGE based identification methods | |
| CN102021126B (en) | Salt-resistant degrading bacterium for treating high-concentration persistent organic wastewater and application thereof | |
| CN104046580B (en) | Sphingobacterium bacterial strain and its application for degrading polycyclic aromatic hydrocarbons class organic pollution | |
| CN104745515A (en) | Acinetobacter sp. for degrading polycyclic aromatic hydrocarbon and application of acinetobacter sp. | |
| CN100439491C (en) | Globular bacillus and its use |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141210 Termination date: 20171030 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |