CN102358942B - Preparation method for biological component of biological compound degreaser - Google Patents
Preparation method for biological component of biological compound degreaser Download PDFInfo
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- CN102358942B CN102358942B CN 201110286685 CN201110286685A CN102358942B CN 102358942 B CN102358942 B CN 102358942B CN 201110286685 CN201110286685 CN 201110286685 CN 201110286685 A CN201110286685 A CN 201110286685A CN 102358942 B CN102358942 B CN 102358942B
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- biological
- oil
- zoogloea
- degreaser
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Abstract
Relating to the technical field of surface degreasing of metallic materials, the invention provides a preparation method for a biological component of a biological compound degreaser. The method comprises the steps of: conducting screening, enriching, domestication and culturing respectively to zoogloea, Bacillus mojavensis, bacillus subtilis, Serratia marcescens, then taking each cultured bacterium in proportion so as to prepare the biological component of a biological compound degreaser. The biological component prepared by the selected 4 bacteria in the invention can degrade mixed oil stains of punching oil, cutting oil, lubricating oil, animal oil and a plurality of oil, and can realize rapid and comprehensive degradation as well as excellent degrading effects.
Description
Technical field:
The present invention relates to metal material surface defatting technology field, relate in particular to a kind of compound method of biological compound type degreasing agent biological components.
Background technology:
Before processing, electroplate and spread at metallochemistry, remove grease and mineral oil on the metallic surface with grease-removing agent.Main grease-removing agent has following several: (1) alkali cleaner is comprised of alkali, sequestrant and tensio-active agent; (2) emulsion grease-removing agent utilizes wettability, impregnability, emulsifying property and the dispersed dirt that can remove the metallic surface of tensio-active agent.General one or several mixtures that adopt in the tensio-active agents such as anionic, cationic, non-ionic type, and dilute with water is made emulsion; (3) pickling process or steaming process degreasing are adopted in solvent degreasing agent.The main solvent that adopts has halogenated hydrocarbon solvent and hydrofluoric ether solvent etc.All environmental protection not in existing these grease-removing agent use procedures, the greasy dirt after emulsification can not in time be decomposed.
The greasy dirt of metallic surface is mixed by punching oil, machining oil, lubricating oil and the multiple grease of animal wet goods often, single culture this kind mixing oil of often can not degrading fast and comprehensively.
Summary of the invention:
Purpose of the present invention is exactly the defects for prior art, and a kind of compound method that can decompose the biological compound type degreasing agent biological components of multiple greasy dirt is provided.
For realizing purpose of the present invention, technical scheme realizes in the following way:
A kind of compound method of biological compound type degreasing agent biological components, it is characterized in that: at first select 4 kinds of bacteriums, after single culture, proportionally be mixed with the biological components mixed solution, described 4 kinds of bacteriums are respectively: Zoogloea, Mo Haiwei genus bacillus, subtilis, serratia marcescens; The method of microbial culture is: respectively to Zoogloea, Mo Haiwei genus bacillus, subtilis, serratia marcescens screen, enrichment, domestication, 32 ℃～36 ℃ of temperature, 24h is cultivated under the condition of 140r/min in PH6.5～8.5.
The configuration proportion of described each bacterium of biological components mixed solution is, Zoogloea 2.5～3mL, Mo Haiwei genus bacillus 4～5mL, subtilis 2～2.5mL, serratia marcescens 3～4mL.
Zoogloea C12～C16 the hydrocarbon in greasy dirt of can degrading, the Mo Haiwei genus bacillus C12～C22 hydrocarbon in greasy dirt of can degrading, the serratia marcescens C20～C28 hydrocarbon in greasy dirt of can degrading, subtilis can purify water, and decomposes the organism such as animal grease.
Be used for the preparation of the combination liquid nutrient medium of microbial culture: glucose 30.0g, peptone 10.0g, yeast extract paste 5.0g, potassium primary phosphate 4.5g, calcium oxide 2.0g, ferrous sulfate 0.2g, sal epsom 1.0g, mineral oil 10.0g, sterilized water 1000ml.
The present invention selects 4 kinds of bacteriums single culture respectively, then mix according to a certain percentage, the advantage that integrates various bacteria, thereby obtain degraded and mix the splendid mixed bacterial of greasy dirt effect, simultaneously, the bio-surfactant that flora produces in process of growth has the effect of emulsification greasy dirt, make the more effective utilization of flora, degraded emulsified greasy dirt and raised growth, degraded greasy dirt effective.
Embodiment:
For the present invention is described in detail, be described further below in conjunction with specific embodiment,
Embodiment one
At first, respectively to Zoogloea, Mo Haiwei genus bacillus, subtilis, serratia marcescens screen, enrichment, domestication, 32 ℃～36 ℃ of temperature, 24h is cultivated under the condition of 140r/min in PH6.5～8.5.
Then get the Zoogloea 2.6mL of cultivation, Mo Haiwei genus bacillus 4.3mL, subtilis 2.3mL, serratia marcescens 3.5mL mixes, and makes the required biological components of 1L biological compound grease-removing agent.
Embodiment two
At first, respectively to Zoogloea, Mo Haiwei genus bacillus, subtilis, serratia marcescens screen, enrichment, domestication, 32 ℃～36 ℃ of temperature, 24h is cultivated under the condition of 140r/min in PH6.5～8.5.
Then get the Zoogloea 5.5mL of cultivation, Mo Haiwei genus bacillus 9mL, subtilis 4.5mL, serratia marcescens 7mL mixes, and makes the required biological components of 2L biological compound grease-removing agent.
Above demonstration and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should understand; the present invention is not restricted to the described embodiments; what describe in above-described embodiment and specification sheets is only preference of the present invention; be not used for limiting the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (2)
1. the compound method of a biological compound type degreasing agent biological components, it is characterized in that: at first select 4 kinds of bacteriums, after single culture, proportionally be mixed with the biological components mixed solution, described 4 kinds of bacteriums are respectively: Zoogloea, Mo Haiwei genus bacillus, subtilis, serratia marcescens; The method of microbial culture is: respectively to Zoogloea, Mo Haiwei genus bacillus, subtilis, serratia marcescens screen, enrichment, domestication, 32 ℃～36 ℃ of temperature, 24h is cultivated under the condition of 140r/min in PH6.5～8.5.
2. the compound method of biological compound type degreasing agent biological components according to claim 1, it is characterized in that: the configuration proportion of described each bacterium of biological components mixed solution is, Zoogloea 2.5～3mL, Mo Haiwei genus bacillus 4～5mL, subtilis 2～2.5mL, serratia marcescens 3～4mL.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110286685 CN102358942B (en) | 2011-09-23 | 2011-09-23 | Preparation method for biological component of biological compound degreaser |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110286685 CN102358942B (en) | 2011-09-23 | 2011-09-23 | Preparation method for biological component of biological compound degreaser |
Publications (2)
| Publication Number | Publication Date |
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| CN102358942A CN102358942A (en) | 2012-02-22 |
| CN102358942B true CN102358942B (en) | 2013-06-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201110286685 Expired - Fee Related CN102358942B (en) | 2011-09-23 | 2011-09-23 | Preparation method for biological component of biological compound degreaser |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103555626B (en) * | 2013-10-30 | 2014-12-10 | 南京师范大学 | Serratia marcescens S2 and application of serratia marcescens S2 in degradation of dibenzothiophene |
| CN105177609B (en) * | 2015-10-01 | 2018-05-25 | 东莞市宏锦金属制品有限公司 | A kind of preparation method of microbial degreasing agent |
| CN105802749B (en) * | 2016-03-17 | 2019-04-30 | 中创宏远（北京）环保科技有限公司 | A kind of greasy dirt detergent and preparation method containing microbial strains |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH06146036A (en) * | 1992-07-23 | 1994-05-27 | Nippon Synthetic Chem Ind Co Ltd:The | Metal surface detergent |
| JPH11323391A (en) * | 1998-05-19 | 1999-11-26 | Yuken Kogyo Kk | Aqueous degreasing bath for metallic article and method for operating same |
| AU2002210793A1 (en) * | 2000-10-17 | 2002-04-29 | Earth Alive Resources Inc. | Cleaning solution to remove hydrocarbons from a substrate |
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2011
- 2011-09-23 CN CN 201110286685 patent/CN102358942B/en not_active Expired - Fee Related
Non-Patent Citations (2)
| Title |
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| JP特开平11-323391A 1999.11.26 |
| JP特开平6-146036A 1994.05.27 |
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| CN102358942A (en) | 2012-02-22 |
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Denomination of invention: Preparation method for biological component of biological compound degreaser Effective date of registration: 20140505 Granted publication date: 20130626 Pledgee: Bengbu financing guarantee Group Co., Ltd. Pledgor: Bengbu Yucheng New Material Technology Co., Ltd. Registration number: 2014990000318 |
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