CN103553773A - Biological anti-freezing agent and preparation method thereof - Google Patents
Biological anti-freezing agent and preparation method thereof Download PDFInfo
- Publication number
- CN103553773A CN103553773A CN201310501582.7A CN201310501582A CN103553773A CN 103553773 A CN103553773 A CN 103553773A CN 201310501582 A CN201310501582 A CN 201310501582A CN 103553773 A CN103553773 A CN 103553773A
- Authority
- CN
- China
- Prior art keywords
- biological
- algae
- frostproofer
- organic solvent
- polar organic
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a biological anti-freezing agent and a preparation method thereof, relates to the technical field of biology, and particularly relates to an anti-freezing agent using a seaweed extract as a main component and a preparation method thereof. The biological anti-freezing agent is characterized by comprising the following components: (1), a water-soluble active ingredient obtained by adding seaweed dry powder capable of generating an anti-freezing active substance into a polar organic solvent through the processes such as extracting, filtering, centrifuging, distilling, freezing and drying; (2), a fat-soluble active ingredient obtained by using the filter residue obtained by filtering in the step as the material, and adding the material into a non-polar organic solvent through extracting, filtering, centrifuging, distilling, freezing and drying; (3), a polar solvent and a non-polar solvent; (4), urea; (5), a surfactant. The biological anti-freezing agent disclosed by the invention has positive effects that the anti-freezing effect is obvious, and the ecological environment is not damaged.
Description
Technical field
The present invention relates to biological technical field, particularly belong to a kind of frostproofer that Seaweed Extract is main ingredient and preparation method thereof of take.
Background technology
North tealeaves take that fragrance is dense, enduring repeated infusions is subject to human consumer's welcome as characteristic.But because area, north of the Changjiang River latitude is high, temperature is low, minimum reaching-15 ℃, and the cold time length is long, is unfavorable for approximately annual 80～90 days time of growth of tea plant.Data shows according to statistics, and north of the Changjiang River tea tree, under-1 ℃～5 ℃ temperature, will suffer the loss of the underproduction, has saying of " little freezing every year having, and freezes greatly and has two for 3 years ".In the common time, tea tree is frozen the dead one two one-tenth, and as the time in Great Cold, tea tree is frozen the dead ten six or seven, causes the spring tea output of next year more than 50%, to cause very big loss to tea grower and local economy the underproduction.The measure of prevention tea tree freeze injury is mainly the freeze proof kind of seed selection and facilities protection at present, but freeze proof kind only works within the specific limits, and facilities protection investment is large, is unfavorable for large-area applications.Once attempted carrying out windproof, insulation with pine branch, shallow basket branch, straw curtain, but by there being thereon cypress to draw the worm's ovums such as sarcoptic mite, planthopper, blue leafhopper, red spider, easily transfer on tea tree, thereby bring serious disease and pest, so have to abandon.
Summary of the invention
The object of the present invention is to provide a kind of novel biological frostproofer and preparation method thereof, reach the object of utilizing the contained active substance of marine alga to manufacture biological frostproofer.
Biological frostproofer provided by the present invention, it is characterized in that, its component is: 1) can produce the seaweed meal of antifreeze active substance, add polar organic solvent to obtain water-soluble effective ingredient by extraction, filtration, centrifugal, distillation, lyophilize supervisor.2) take in above-mentioned steps and filter gained filter residue as raw material, add non-polar organic solvent, through extraction, filtration, centrifugal, distillation, lyophilize, obtain fat-soluble effective constituent; 3) polar solvent and non-polar solvent; 4) urea; 5) tensio-active agent.
The described marine alga that can produce antifreeze active substance is anabena, synnema algae, simple spore algae, Microcystis aeruginosa, joint ball algae, nostoc, Fei Shi algae, the algae that quivers, splitting must algae, the microcystic aeruginosa in fake valoniopsis pachynema, spirulina, single rugged algae, Shu Maozao, blue-green algae, the combination of wherein any one or a few.
Described polar organic solvent is methyl alcohol, ethanol, acetic acid; Non-polar organic solvent is ether, acetone, chloroform, sherwood oil, acetyl triethyl, tensio-active agent is any crude substance that can increase activeconstituents stability and dispersity, such as amino acid, grease and soft phosphatide, Mierocrystalline cellulose, xylogen, chitin etc., or the derivative of above-mentioned substance.
The preparation method of biological frostproofer provided by the present invention, is characterized in that, comprises the following steps:
Step 1, utilization can produce the marine alga of antifreeze active substance, adds polar organic solvent, through extraction, filtration, centrifugal, distillation, lyophilize, obtains water-soluble effective ingredient standby.
Step 2, the filter residue that step 1 obtains adds non-polar solvent, through extraction, filtration, centrifugal, distillation, lyophilize, obtains fat-soluble effective constituent standby.
Step 3, step 1 gained aqueous soluble active constituent is dissolved in polar organic solvent, the fat-soluble effective constituent of step 2 gained is dissolved in non-polar solvent, two kinds of solution of gained mix, add 5-20% urea, 1-10% tensio-active agent, regulates concentration, make effective constituent account for the 1-45% of weight, be biological frostproofer disclosed in this invention.
Biological frostproofer disclosed in this invention, take Seaweed Extract as main ingredient, due to adaptable to low temperature of marine alga, the synthetic biomacromolecule of marine alga particularly polyunsaturated fatty acid has the ability that improves frost resistance, dilute with water 100-1000 is rear enforcement foliage spray doubly, tea tree cold damage or the freeze injury that can effectively prevent northern low temperature or cold current to cause.There is the positively effect that antifreezing effect obviously, does not destroy ecotope.
Embodiment
Example one, the extraction of the antifreeze activeconstituents of marine alga:
Select respectively anabena, synnema algae, simple spore algae, Microcystis aeruginosa, joint ball algae, nostoc, Fei Shi algae, algae quivers, split palpus algae, fake valoniopsis pachynema, spirulina, single rugged algae, Shu Maozao, or 5 kilograms of microcystic aeruginosa siccatives in blue-green algae, grind to form dry powder, adds 10 liters of dehydrated alcohols to extract at ambient temperature 4 hours, filter, with 3 liters of flushings of dehydrated alcohol, merging filtrate, under 3000 rpm conditions centrifugal 5 minutes, centrifugate was distilled under 70 degree, and concentrated solution obtains water-soluble active ingredient through lyophilize.Gained filter residue is with doubly measuring chloroform extraction 4 hours, filters, with chloroform, rinse three times, and merging filtrate under 3000 rpm conditions centrifugal 5 minutes, centrifugate is distilled under 50 degree, and concentrated solution obtains fat-soluble active ingredient through lyophilize.
Example two, the compound method of biological frostproofer:
15 grams of the water-soluble components obtaining with 30 milliliters of anhydrous alcohol solution examples one, 15 grams of the fat-soluble active ingredients obtaining by 30 milliliters of chloroform dissolution examples one, after mixing, add 8 grams, sterling urea, fatty acid glycerine fat is 6 grams, the tensio-active agent of main body, finally with dehydrated alcohol, is settled to 100 milliliters.Obtain thus activeconstituents and account for 30% biological frostproofer.
The biological frostproofer effect measuring of example three:
For trial product be above obtain biological frostproofer, for studying thing, be that the age of tree is the tea tree of 5 years, 100 of every group of processing, totally 6 groups.After the Frost's Descent, by 500 times of product dilutions, every 5 days, spray once continuous spraying three times.After the Waking of Insects (3rd solar term), at warm calm weather, every 3-5 days, spray once again continuous spraying three times.Contrast with equivalent clear water foliage-spray.Early May statistics tea tree is subject to the extent of injury of freeze injury strain number and every strain.Every strain extent of injury is divided into 5 grades: 0 grade, without freeze injury; 1 grade, 1-20% freeze injury; 2 grades, 21-50% freeze injury; 3 grades, 51-75% freeze injury; 4 grades, 76-100% freeze injury.
Statistical analysis, all trial products that supply all show remarkable antifreeze cryoprotective effects, and result is as follows.
Above-described embodiment is only used for illustrating design of the present invention and feature, can not limit the scope of the invention.Any variation of doing according to the principle of the invention or modification, all within protection scope of the present invention.
Claims (6)
1. a biological frostproofer, is characterized in that, its component is: 1) can produce the seaweed meal of antifreeze active substance, add polar organic solvent to obtain water-soluble effective ingredient by extraction, filtration, centrifugal, distillation, lyophilize supervisor; 2) take in above-mentioned steps and filter gained filter residue as raw material, add non-polar organic solvent, through extraction, filtration, centrifugal, distillation, lyophilize, obtain fat-soluble effective constituent; 3) polar solvent and non-polar solvent; 4) urea; 5) tensio-active agent.
2. a preparation method for biological frostproofer, is characterized in that, comprises the following steps:
Step 1, utilization can produce the marine alga of antifreeze active substance, adds polar organic solvent, through extraction, filtration, centrifugal, distillation, lyophilize, obtains water-soluble effective ingredient standby;
Step 2, the filter residue that step 1 obtains adds non-polar solvent, through extraction, filtration, centrifugal, distillation, lyophilize, obtains fat-soluble effective constituent standby;
Step 3, step 1 gained aqueous soluble active constituent is dissolved in polar organic solvent, the fat-soluble effective constituent of step 2 gained is dissolved in non-polar solvent, two kinds of solution of gained mix, add 5-20% urea, 1-10% tensio-active agent, regulates concentration, make effective constituent account for the 1-45% of weight, be biological frostproofer disclosed in this invention.
3. biological frostproofer according to claim 1, be further characterized in that, the described marine alga that can produce antifreeze active substance is anabena, synnema algae, simple spore algae, Microcystis aeruginosa, joint ball algae, nostoc, Fei Shi algae, the algae that quivers, splitting must algae, the microcystic aeruginosa in fake valoniopsis pachynema, spirulina, single rugged algae, Shu Maozao, blue-green algae.
4. biological frostproofer according to claim 1, is further characterized in that, described polar organic solvent is methyl alcohol, ethanol, acetic acid.
5. biological frostproofer according to claim 1, is further characterized in that, described non-polar organic solvent is ether, acetone, chloroform, sherwood oil, acetyl triethyl.
6. biological frostproofer according to claim 1, is further characterized in that, described tensio-active agent is amino acid, grease, soft phosphatide, Mierocrystalline cellulose, xylogen, chitin, or the derivative of above-mentioned substance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310501582.7A CN103553773A (en) | 2013-10-23 | 2013-10-23 | Biological anti-freezing agent and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310501582.7A CN103553773A (en) | 2013-10-23 | 2013-10-23 | Biological anti-freezing agent and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103553773A true CN103553773A (en) | 2014-02-05 |
Family
ID=50008192
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310501582.7A Pending CN103553773A (en) | 2013-10-23 | 2013-10-23 | Biological anti-freezing agent and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103553773A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108727114A (en) * | 2018-08-13 | 2018-11-02 | 威海长青海洋科技股份有限公司 | A kind of Plant antifreezer and preparation method thereof prepared using seaweed extracted liquor |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1133673A (en) * | 1995-04-17 | 1996-10-23 | 樊双泉 | Flower bud antifreezing dope for vegetables and fruit trees |
| CN101438729A (en) * | 2008-12-18 | 2009-05-27 | 上海海洋大学 | Natural deicing fluid for freezing shrimp meat |
| CN101965830A (en) * | 2010-10-18 | 2011-02-09 | 西北农林科技大学 | Anti-freezing agent and anti-freezing diluent for freezing and storing livestock sperm and preparation method thereof |
| CN102388924A (en) * | 2011-08-28 | 2012-03-28 | 鞠志国 | Biological insecticide and preparation method thereof |
-
2013
- 2013-10-23 CN CN201310501582.7A patent/CN103553773A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1133673A (en) * | 1995-04-17 | 1996-10-23 | 樊双泉 | Flower bud antifreezing dope for vegetables and fruit trees |
| CN101438729A (en) * | 2008-12-18 | 2009-05-27 | 上海海洋大学 | Natural deicing fluid for freezing shrimp meat |
| CN101965830A (en) * | 2010-10-18 | 2011-02-09 | 西北农林科技大学 | Anti-freezing agent and anti-freezing diluent for freezing and storing livestock sperm and preparation method thereof |
| CN102388924A (en) * | 2011-08-28 | 2012-03-28 | 鞠志国 | Biological insecticide and preparation method thereof |
Non-Patent Citations (2)
| Title |
|---|
| 王勇等: "螺旋藻类蛋白-有机醇复合防冻剂的制备研究", 《材料导报》 * |
| 黄永和: "茶树喷施"海藻"植物营养液试验初报", 《福建茶叶》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108727114A (en) * | 2018-08-13 | 2018-11-02 | 威海长青海洋科技股份有限公司 | A kind of Plant antifreezer and preparation method thereof prepared using seaweed extracted liquor |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Sangwan et al. | A review on Pongamia pinnata (L.) Pierre: A great versatile leguminous plant | |
| Girish et al. | Neem–a green treasure | |
| Dhyani et al. | Basic nutritional attributes of Hippophae rhamnoides (Seabuckthorn) populations from Uttarakhand Himalaya, India | |
| Gioele et al. | Gracilaria gracilis, source of agar: A short review | |
| CN102613281B (en) | Preparation method for south China pine needle extractives and application of south China pine needle extractive in freshness of eggs | |
| CN107438427A (en) | A kind of natural shampoo | |
| CN103814954A (en) | Rhodiola rosea extract product and preparation method and application thereof | |
| Singh | Endangered economic species of Indian desert | |
| CN104336085B (en) | Ligularia virgaurea (Maxim.) Mattf. Ex Rehd. Etkobuski extract insecticide and preparation method thereof | |
| US20110015411A1 (en) | Inhibitor for growth of bacteria of genus legionella, bath agent and cleaning agent | |
| KR101153181B1 (en) | A Method of Preparing the Insecticidal Extract of Sophorae Radix for Moth, the Agents comprising it and the Method of Controling Moth | |
| CN102415416B (en) | Method for preparing alga inhibitor from barley | |
| Demirel et al. | Biochemical analysis of some brown seaweeds from the Aegean Sea | |
| CN103553773A (en) | Biological anti-freezing agent and preparation method thereof | |
| CN101491260A (en) | Preparation method of female medicine of Mafeng trees | |
| Ekka et al. | Madhuca longifolia var. latifolia: an important medicinal plant used by tribes of north-east part of Chhattisgarh | |
| CN103599162A (en) | Application of Tibet rhodiola crenulata extract in preparing medicament for resisting intestinal inflammation | |
| Shimomura et al. | Identification of the plant origin of propolis from Jeju Island, Korea, by observation of honeybee behavior and phytochemical analysis | |
| Machado et al. | Triagem de macroalgas com potencial antifúngico no controle in vitro da antracnose do mamoeiro (Carica papaya L.) | |
| CN112244042B (en) | Application of herba delphinii or extract thereof in preventing and treating sisalana ananas comosus | |
| CN110818811B (en) | Method for extracting and separating high-viscosity narcissus polysaccharide from narcissus bulb | |
| KR20180024301A (en) | A composition for preventing rice sheath blight comprising extracts of Aesculus turbinate | |
| CN106045956A (en) | Method for extracting and separating algal inhibiting substance and application thereof | |
| CN105153320A (en) | Laminarin extraction method | |
| Balandrán-Quintana et al. | An overview of cholla (Cylindropuntia spp.) from Sonora, Mexico |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140205 |