CN103549386B - A kind of compound Chinese caterpillar fungus-lucid ganoderma composition and preparation method thereof - Google Patents
A kind of compound Chinese caterpillar fungus-lucid ganoderma composition and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of compound Chinese caterpillar fungus-lucid ganoderma composition and preparation method thereof, in said composition, the weight portion of each composition is: cordyceps 100-300 weight portion, ganoderma lucidum powder 100-220 weight portion, manyzoned polypore bacteria powder 100-220 weight portion.Compound Chinese caterpillar fungus-lucid ganoderma composition of the present invention can significantly rise unit and consolidate, and increases immunity of organisms, has the effect of adjunct antineoplastic.
Description
Technical field
The present invention relates to a kind of compound Chinese caterpillar fungus-lucid ganoderma composition and preparation method thereof, belong to biomedicine field.
Background technology
At present, on market, health products are of a great variety, mostly have and improve immunity, anti-oxidant, anti-ageing effect of waiting for a long time, but these health products mostly add chemicals to promote drug effect, people's short-term is taken can not exist any harm, but just there will be various different side effect after long-term taking.
Summary of the invention
The object of this invention is to provide a kind of formula simple, dose is low but can significantly improve body immunity, improves microcirculation, the compound Chinese caterpillar fungus-lucid ganoderma composition delayed senility.
The object of the invention is to realize in the following manner:
A kind of compound Chinese caterpillar fungus-lucid ganoderma composition, is characterized in that the weight portion of each composition in said composition is:
Cordyceps 100-300 weight Ganoderma Lucidum bacterium powder (also known as Ganoderma lucidum mycelium powder) 100-220 weight portion manyzoned polypore bacteria powder 100-220 weight portion.
In preferred said composition, the weight portion of each composition is:
Cordyceps 100-250 weight Ganoderma Lucidum bacterium powder 150-220 weight portion manyzoned polypore bacteria powder 100-200 weight portion.
In preferred said composition, the weight portion of each composition is:
Cordyceps 100-250 weight Ganoderma Lucidum bacterium powder 100-200 weight portion manyzoned polypore bacteria powder 150-220 weight portion.
In preferred said composition, the weight portion of each composition is:
Cordyceps 100-300 weight Ganoderma Lucidum bacterium powder 100-200 weight portion manyzoned polypore bacteria powder 100-220 weight portion.
Cordyceps sinensis polysaccharide >=1.5% in above-mentioned cordyceps, GL-B >=1.5% in ganoderma lucidum powder, coriolan >=4.0% in manyzoned polypore bacteria powder.
The preparation method of above-mentioned compound Chinese caterpillar fungus-lucid ganoderma composition comprises the following steps: cordyceps, ganoderma lucidum powder, manyzoned polypore bacteria powder are mixed in proportion.
The cordyceps that the present invention uses, ganoderma lucidum powder and manyzoned polypore bacteria powder raw material all can be obtained by commercially available acquisition or self-control.
Ganoderma lucidum powder, for brown color is to chocolate brown powder, has the distinctive fragrance of product, salty, the micro-hardship of taste acid, free from extraneous odour, wherein, and GL-B >=1.5g/100g.Cordyceps sinensis polysaccharide >=1.5% in cordyceps, coriolan >=4.0% in manyzoned polypore bacteria powder.Above-mentioned raw materials can also be dried to pulverize by the mycelium of liquid deep layer fermenting and be obtained.
Compound Chinese caterpillar fungus-lucid ganoderma composition of the present invention can make preparation with pharmaceutically acceptable auxiliary material, as: powder, granule, capsule, tablet, oral liquid or injection etc.Compound Chinese caterpillar fungus-lucid ganoderma composition of the present invention effectively can improve body immunity, improve microcirculation, there is antiatherosclerosis, anti-ageing effect of waiting for a long time, can for the preparation of increasing the medicine of immunity of organisms, can also for the preparation of the medicine of effect with adjunct antineoplastic.The present composition and cordyceps, ganoderma lucidum powder, manyzoned polypore bacteria powder one-component or both combine and compare arbitrarily, significantly can reduce dose.
Below by way of concrete test example, the present invention is further described:
Simple cordyceps and the ganoderma lucidum powder of getting this compound Chinese caterpillar fungus glossy ganoderma Mixed Microbes powder and same dosage compare its immunity humidification, have significant difference, the results are shown in Table 1, table 2 and table 3.
Test example 1: on the impact of mouse thymus index (thymus gland average quality mg/ average body quality g)
This test is divided into 7 groups, often organize 20 mouse, forage feed based on its empty group, feed based on simple cordyceps group+simple cordyceps (2g/d) is fed, feed based on simple ganoderma lucidum powder group+simple ganoderma lucidum powder (2g/d) is fed, based on embodiment 2 feed+embodiment 2 fill a prescription (2g/d) feed, based on embodiment 4 feed+embodiment 4 fill a prescription (2g/d) feed, based on embodiment 6 feed+embodiment 6 fill a prescription (2g/d) feed, based on embodiment 8 feed+embodiment 8 fill a prescription (2g/d) feed, the nursing time is 10d, its thymus index is detected after 10d.
Table 1
| Group | Size of animal (only) | Thymus index | P value |
| Blank group | 20 | 14.13±1.07 | / |
| Simple cordyceps group | 20 | 22.87±5.25 | ＜0.05 |
| Simple ganoderma lucidum powder group | 20 | 23.18±6.92 | ＜0.05 |
| Embodiment 2 | 20 | 33.02±8.46 | ＜0.05 |
| Embodiment 4 | 20 | 34.18±7.95 | ＜0.05 |
| Embodiment 6 | 20 | 32.92±7.67 | ＜0.05 |
| Embodiment 8 | 20 | 35.32±9.19 | ＜0.05 |
Conclusion: as can be seen from the above table compared with blank group, simple cordyceps and ganoderma lucidum powder increase Be very effective to mouse thymus index; And compare with ganoderma lucidum powder with simple cordyceps, compound Cordyceps-Ganoderma bacterium powder all has significant difference to the increase of mouse thymus index, Be very effective.
Test example 2: on the impact of mice spleen lymphocytes proliferation
This test is divided into 7 groups, often organize 20 mouse, forage feed based on its empty group, feed based on simple cordyceps group+simple cordyceps (2g/d) is fed, feed based on simple ganoderma lucidum powder group+simple ganoderma lucidum powder (2g/d) is fed, based on embodiment 1 feed+embodiment 1 fill a prescription (2g/d) feed, based on embodiment 3 feed+embodiment 3 fill a prescription (2g/d) feed, based on embodiment 5 feed+embodiment 5 fill a prescription (2g/d) feed, based on embodiment 7 feed+embodiment 7 fill a prescription (2g/d) feed, the nursing time is 10d, its lymphopoiesis situation is detected after 10d.
Table 2
| Group | Size of animal (only) | Optical density difference | P value |
| Blank group | 20 | 0.0517±0.0235 | / |
| Simple cordyceps group | 20 | 0.2753±0.0897 | ＜0.05 |
| Simple ganoderma lucidum powder group | 20 | 0.2688±0.0769 | ＜0.05 |
| Embodiment 1 | 20 | 0.5047±0.0572 | ＜0.05 |
| Embodiment 3 | 20 | 0.5381±0.0497 | ＜0.05 |
| Embodiment 5 | 20 | 0.5649±0.0539 | ＜0.05 |
| Embodiment 7 | 20 | 0.6014±0.0329 | ＜0.05 |
Conclusion: as can be seen from the above table compared with blank group, simple cordyceps and ganoderma lucidum powder are to mice spleen lymphocytes proliferation Be very effective; And compare with ganoderma lucidum powder with simple cordyceps, compound Cordyceps-Ganoderma bacterium powder is to mice spleen lymphocytes proliferation Be very effective.
Test example 3: on the impact of mouse macrophage phagocytic activity
This test is divided into 7 groups, often organize 20 mouse, forage feed based on its empty group, feed based on simple cordyceps group+simple cordyceps (2g/d) is fed, feed based on simple ganoderma lucidum powder group+simple ganoderma lucidum powder (2g/d) is fed, based on embodiment 2 feed+embodiment 2 fill a prescription (2g/d) feed, based on embodiment 3 feed+embodiment 3 fill a prescription (2g/d) feed, based on embodiment 4 feed+embodiment 4 fill a prescription (2g/d) feed, based on embodiment 9 feed+embodiment 9 fill a prescription (2g/d) feed, the nursing time is 10d, its macrophage phagocytic index is detected after 10d.
Table 3
| Group | Size of animal (only) | Phagocytic index | P value |
| Blank group | 20 | 22.54±7.62 | / |
| Simple cordyceps group | 20 | 30.72±8.91 | ＜0.05 |
| Simple ganoderma lucidum powder group | 20 | 32.15±7.39 | ＜0.05 |
| Embodiment 2 | 20 | 35.38±5.64 | ＜0.05 |
| Embodiment 3 | 20 | 35.29±4.39 | ＜0.05 |
| Embodiment 4 | 20 | 36.64±5.86 | ＜0.05 |
| Embodiment 9 | 20 | 37.09±4.85 | ＜0.05 |
Conclusion: as can be seen from the above table compared with blank group, simple cordyceps and ganoderma lucidum powder are to mouse macrophage phagocytic activity Be very effective; And compare with ganoderma lucidum powder with simple cordyceps, compound Cordyceps-Ganoderma bacterium powder is to mouse macrophage phagocytic activity Be very effective.
It can thus be appreciated that the increase immunity of organisms of the present composition, the simple addition of effect not each constituent function with adjunct antineoplastic are the synergistic functions of each constituent.In addition, the present composition is placed about 6 months under normal temperature condition, and its active constituent content is substantially constant.
Detailed description of the invention:
The present invention is further illustrated below by way of specific embodiment.But the detail of embodiment only for explaining the present invention, should not be construed as limited overall technical solution.
Cordyceps deep liquid fermentation process:
Cordyceps bacterial strain: Cordyceps Militaris-Paecilomyces hepiali chen (Paecilomyces hepiali chen) Cs-4, buys in Chinese Academy of Sciences's Culture Collection.
The preservation bacterial classification taken out from refrigerator is connected on fresh slant medium, and culture medium prescription is: potato 8.0%, glucose 3.0%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.15%, agar 1.3%, cultivates 4 days for 26 DEG C.
On inclined-plane, cultured strain transfer is in shaking flask, and Shake flask medium is: glucose 2%, peptone 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, cultivates 2 days for 26 DEG C.
After Shaking culture terminates, by strain transfer in seeding tank, seed tank culture base is: glucose 2%, peptone 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, defoamer 0.1%, technological condition for fermentation is: inoculum concentration 10%, temperature 26 DEG C ± 1 DEG C, throughput 1: 0.5vvm, tank pressure 0.03MPa.
Seeding tank is transferred to bulk fermentation after fermenting 3 days, and large tank culture medium is: glucose 1.5%, peptone 2%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.07%, defoamer 0.1%.Zymotechnique is: inoculum concentration 10%, cultivation temperature 26 DEG C ± 1 DEG C, ventilation 1: 0.5vvm, tank pressure 0.03MPa.
After fermentation ends, fermentation liquor horizontal spiral discharge sedimentation centrifuge is separated, and mycelium vacuum drying below 70 DEG C, pulverized 100 mesh sieves and namely obtain cordyceps after oven dry.
Ganoderma lucidum powder deep liquid fermentation process:
Ganoderma lucidum powder bacterial strain: glossy ganoderma [Ganoderma lucidum(Curtis:Fr) P.Karst.], buy in Chinese Academy of Sciences's Culture Collection.
The preservation bacterial classification taken out from refrigerator is connected on fresh slant medium, and culture medium prescription is: potato 8%, glucose 2.0%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.08%, agar 1.5%, cultivates 3 days for 25 DEG C.
On inclined-plane, cultured strain transfer is in shaking flask, and Shake flask medium is: soybean cake powder 1.0%, sucrose 3.0%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.075%, ammonium sulfate 0.05%, cultivates 2 days for 25 DEG C.
After Shaking culture terminates, by strain transfer in seeding tank, seed tank culture base is: soybean cake powder 1%, glucose 2%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, defoamer 0.1%, technological condition for fermentation is: inoculum concentration 10%, temperature 26 DEG C ± 1 DEG C, throughput 1: 0.5vvm, tank pressure 0.03MPa.
Seeding tank is transferred to bulk fermentation after fermenting 2 days, and large tank culture medium is: soybean cake powder 2.0%, dried silkworm chrysalis meal 0.5%, glucose 1%, potassium dihydrogen phosphate 0.08% magnesium sulfate 0.07%, defoamer 0.1%.Zymotechnique is: inoculum concentration 10%, cultivation temperature 27 DEG C ± 1 DEG C, ventilation 1: 0.5vvm, tank pressure 0.03MPa.
After fermentation ends, fermentation liquor horizontal spiral discharge sedimentation centrifuge is separated, and mycelium vacuum drying below 70 DEG C, pulverized 100 mesh sieves and namely obtain ganoderma lucidum powder after oven dry.
Manyzoned polypore bacteria powder liquid deep layer fermenting process:
Manyzoned polypore bacteria powder bacterial strain: Coriolus Versicolor [Polystictus Versicolor (L) Fr], buys in Chinese Academy of Sciences's Culture Collection.
The preservation bacterial classification taken out from refrigerator is connected on fresh slant medium, and culture medium prescription is: potato 10.0%, glucose 2.0%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.15%, agar 1.3%, cultivates 5 days for 26 DEG C.
On inclined-plane, cultured strain transfer is in shaking flask, and Shake flask medium is: soybean cake powder 1.0%, glucose 2%, peptone 0.5%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, cultivates 3 days for 26 DEG C.
After Shaking culture terminates, by strain transfer in seeding tank, seed tank culture base is: glucose 2%, peptone 1%, sodium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, defoamer 0.1%, technological condition for fermentation is: inoculum concentration 10%, temperature 26 DEG C ± 1 DEG C, throughput 1: 0.5vvm, tank pressure 0.03MPa.
Seeding tank is transferred to bulk fermentation after fermenting 3 days, and large tank culture medium is: glucose 2%, soybean cake powder 2%, peptone 1%, sodium dihydrogen phosphate 0.4%, magnesium sulfate 0.05%, defoamer 0.1%, zymotechnique is: inoculum concentration 15%, cultivation temperature 26 DEG C ± 1 DEG C, throughput 1: 0.5vvm; Tank pressure 0.03MPa.
After fermentation ends, fermentation liquor horizontal spiral discharge sedimentation centrifuge is separated, and mycelium vacuum drying below 70 DEG C, pulverized 100 mesh sieves and namely obtain manyzoned polypore bacteria powder after oven dry.
Its material quality index of cordyceps, ganoderma lucidum powder and manyzoned polypore bacteria powder is as follows:
Embodiment 1
Cordyceps 100mg, ganoderma lucidum powder 220mg, manyzoned polypore bacteria powder 180mg; Three kinds of components mix and get final product.
Embodiment 2
Cordyceps 100mg, ganoderma lucidum powder 180mg, manyzoned polypore bacteria powder 220mg; Three kinds of components mix and get final product.
Embodiment 3
Cordyceps 150mg, ganoderma lucidum powder 150mg, manyzoned polypore bacteria powder 200mg; Three kinds of components mix and get final product.
Embodiment 4
Cordyceps 150mg, ganoderma lucidum powder 200mg, manyzoned polypore bacteria powder 150mg; Three kinds of components mix and get final product.
Embodiment 5
Cordyceps 200mg, ganoderma lucidum powder 150mg, manyzoned polypore bacteria powder 150mg; Three kinds of components mix and get final product.
Embodiment 6
Cordyceps 250mg, ganoderma lucidum powder 100mg, manyzoned polypore bacteria powder 150mg; Three kinds of components mix and get final product.
Embodiment 7
Cordyceps 250mg, ganoderma lucidum powder 150mg, manyzoned polypore bacteria powder 100mg; Three kinds of components mix and get final product.
Embodiment 8
Cordyceps 250mg, ganoderma lucidum powder 100mg, manyzoned polypore bacteria powder 150mg; Three kinds of components mix and get final product.
Embodiment 9
Cordyceps 300mg, ganoderma lucidum powder 100mg, manyzoned polypore bacteria powder 100mg; Three kinds of components mix and get final product.
Claims (6)
1. a preparation method for compound Chinese caterpillar fungus-lucid ganoderma composition, is characterized in that each component being mixed and stirring: component is:
Cordyceps 100-300 weight Ganoderma Lucidum bacterium powder 100-220 weight portion manyzoned polypore bacteria powder 100-220 weight portion; Cordyceps sinensis polysaccharide >=1.5% in cordyceps, GL-B >=1.5% in ganoderma lucidum powder, coriolan >=4.0% in manyzoned polypore bacteria powder; Wherein,
Cordyceps deep liquid fermentation process is as follows:
Getting Cordyceps Militaris-Paecilomyces hepiali chen Cs-4 bacterial classification is connected on fresh slant medium, and culture medium prescription is: potato 8.0%, glucose 3.0%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.15%, agar 1.3%, cultivates 4 days for 26 DEG C; On inclined-plane, cultured strain transfer is in shaking flask, and Shake flask medium is: glucose 2%, peptone 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, cultivates 2 days for 26 DEG C; After Shaking culture terminates, by strain transfer in seeding tank, seed tank culture base is: glucose 2%, peptone 1%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, defoamer 0.1%, technological condition for fermentation is: inoculum concentration 10%, temperature 26 DEG C ± 1 DEG C, throughput 1: 0.5vvm, tank pressure 0.03MPa; Seeding tank is transferred to bulk fermentation after fermenting 3 days, and large tank culture medium is: glucose 1.5%, peptone 2%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.07%, defoamer 0.1%, zymotechnique is: inoculum concentration 10%, cultivation temperature 26 DEG C ± 1 DEG C, ventilation 1: 0.5vvm, tank pressure 0.03MPa; After fermentation ends, fermentation liquor horizontal spiral discharge sedimentation centrifuge is separated, and mycelium vacuum drying below 70 DEG C, pulverized 100 mesh sieves and namely obtain cordyceps after oven dry;
Ganoderma lucidum powder deep liquid fermentation process is as follows:
Getting lucidum strain is connected on fresh slant medium, and culture medium prescription is: potato 8%, glucose 2.0%, potassium dihydrogen phosphate 0.2%, magnesium sulfate 0.08%, agar 1.5%, cultivates 3 days for 25 DEG C; On inclined-plane, cultured strain transfer is in shaking flask, and Shake flask medium is: soybean cake powder 1.0%, sucrose 3.0%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.075%, ammonium sulfate 0.05%, cultivates 2 days for 25 DEG C; After Shaking culture terminates, by strain transfer in seeding tank, seed tank culture base is: soybean cake powder 1%, glucose 2%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, defoamer 0.1%, technological condition for fermentation is: inoculum concentration 10%, temperature 26 DEG C ± 1 DEG C, throughput 1: 0.5vvm, tank pressure 0.03MPa; Seeding tank is transferred to bulk fermentation after fermenting 2 days, large tank culture medium is: soybean cake powder 2.0%, dried silkworm chrysalis meal 0.5%, glucose 1%, potassium dihydrogen phosphate 0.08% magnesium sulfate 0.07%, defoamer 0.1%, zymotechnique is: inoculum concentration 10%, cultivation temperature 27 DEG C ± 1 DEG C, ventilation 1: 0.5vvm, tank pressure 0.03MPa; After fermentation ends, fermentation liquor horizontal spiral discharge sedimentation centrifuge is separated, and mycelium vacuum drying below 70 DEG C, pulverized 100 mesh sieves and namely obtain ganoderma lucidum powder after oven dry;
Manyzoned polypore bacteria powder liquid deep layer fermenting process is as follows:
Getting Coriolus Versicolor bacterial classification is connected on fresh slant medium, and culture medium prescription is: potato 10.0%, glucose 2.0%, potassium dihydrogen phosphate 0.3%, magnesium sulfate 0.15%, agar 1.3%, cultivates 5 days for 26 DEG C; On inclined-plane, cultured strain transfer is in shaking flask, and Shake flask medium is: soybean cake powder 1.0%, glucose 2%, peptone 0.5%, potassium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, cultivates 3 days for 26 DEG C; After Shaking culture terminates, by strain transfer in seeding tank, seed tank culture base is: glucose 2%, peptone 1%, sodium dihydrogen phosphate 0.1%, magnesium sulfate 0.05%, defoamer 0.1%, technological condition for fermentation is: inoculum concentration 10%, temperature 26 DEG C ± 1 DEG C, throughput 1: 0.5vvm, tank pressure 0.03MPa; Seeding tank is transferred to bulk fermentation after fermenting 3 days, and large tank culture medium is: glucose 2%, soybean cake powder 2%, peptone 1%, sodium dihydrogen phosphate 0.4%, magnesium sulfate 0.05%, defoamer 0.1%, zymotechnique is: inoculum concentration 15%, cultivation temperature 26 DEG C ± 1 DEG C, throughput 1: 0.5vvm; Tank pressure 0.03MPa; After fermentation ends, fermentation liquor horizontal spiral discharge sedimentation centrifuge is separated, and mycelium vacuum drying below 70 DEG C, pulverized 100 mesh sieves and namely obtain manyzoned polypore bacteria powder after oven dry.
2. the preparation method of compound Chinese caterpillar fungus-lucid ganoderma composition according to claim 1, is characterized in that the weight portion of each composition is: cordyceps 100-250 weight Ganoderma Lucidum bacterium powder 150-220 weight portion manyzoned polypore bacteria powder 100-200 weight portion.
3. the preparation method of compound Chinese caterpillar fungus-lucid ganoderma composition according to claim 1, is characterized in that the weight portion of each composition is: cordyceps 100-250 weight Ganoderma Lucidum bacterium powder 100-200 weight portion manyzoned polypore bacteria powder 150-220 weight portion.
4. the preparation method of compound Chinese caterpillar fungus-lucid ganoderma composition according to claim 1, is characterized in that the weight portion of each composition is: cordyceps 100-300 weight Ganoderma Lucidum bacterium powder 100-200 weight portion manyzoned polypore bacteria powder 100-220 weight portion.
5. the preparation method of the compound Chinese caterpillar fungus-lucid ganoderma composition according to claim 1,2,3 or 4, is characterized in that preparation made by said composition and pharmaceutically acceptable auxiliary material.
6. the preparation method of compound Chinese caterpillar fungus-lucid ganoderma composition according to claim 5, is characterized in that described preparation is powder, granule, capsule, tablet, oral liquid or injection.
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| CN104905262B (en) * | 2015-05-29 | 2017-12-22 | 湖北省农业科学院农产品加工与核农技术研究所 | A kind of method for preparing Cordyceps-Ganoderma compound formulation |
| CN104997815A (en) * | 2015-07-08 | 2015-10-28 | 哈尔滨瑞康源生物科技有限公司 | Cordyceps sinensis and Mythic Fungus fermentation powder composite lozenge and preparation method thereof |
| CN107184615A (en) * | 2017-07-12 | 2017-09-22 | 亳州学院 | A kind of adjuvant Chinese medicine composition and preparation method thereof |
| CN108077904A (en) * | 2017-12-21 | 2018-05-29 | 山西佰富康生物科技有限公司 | A kind of Cordyceps militaris nutrition brewed powder and preparation method thereof |
| CN115873718A (en) * | 2022-10-25 | 2023-03-31 | 江苏神华药业有限公司 | Liquid fermentation medium of armillaria mellea mycelium, and preparation method and application thereof |
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| 真菌多糖与人体健康;周小鹭;《黑龙江生态工程职业学院学报》;20090930;第22卷(第5期);第72-74页 * |
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|---|---|
| CN103549386A (en) | 2014-02-05 |
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