CN103535825B - Tumor postoperation healthy drink prepared by utilizing enzymolysis for extracting scallop glycoprotein - Google Patents
Tumor postoperation healthy drink prepared by utilizing enzymolysis for extracting scallop glycoprotein Download PDFInfo
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- CN103535825B CN103535825B CN201310469142.8A CN201310469142A CN103535825B CN 103535825 B CN103535825 B CN 103535825B CN 201310469142 A CN201310469142 A CN 201310469142A CN 103535825 B CN103535825 B CN 103535825B
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- 241000237509 Patinopecten sp. Species 0.000 title claims abstract description 62
- 235000020637 scallop Nutrition 0.000 title claims abstract description 62
- 102000003886 Glycoproteins Human genes 0.000 title claims abstract description 60
- 108090000288 Glycoproteins Proteins 0.000 title claims abstract description 60
- 206010028980 Neoplasm Diseases 0.000 title claims abstract description 39
- 239000003814 drug Substances 0.000 claims abstract description 28
- 235000021552 granulated sugar Nutrition 0.000 claims abstract description 9
- 235000013361 beverage Nutrition 0.000 claims description 10
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 9
- 239000000284 extract Substances 0.000 claims description 8
- 238000000605 extraction Methods 0.000 claims description 7
- 239000000287 crude extract Substances 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 244000189799 Asimina triloba Species 0.000 claims description 5
- 235000006264 Asimina triloba Nutrition 0.000 claims description 5
- 241000132012 Atractylodes Species 0.000 claims description 5
- 235000009467 Carica papaya Nutrition 0.000 claims description 5
- 239000009636 Huang Qi Substances 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 4
- 241001526627 Azumapecten farreri Species 0.000 claims description 3
- GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 3
- 229920005654 Sephadex Polymers 0.000 claims description 3
- 239000012507 Sephadex™ Substances 0.000 claims description 3
- 238000004440 column chromatography Methods 0.000 claims description 3
- 239000003480 eluent Substances 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 102000004169 proteins and genes Human genes 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 238000000746 purification Methods 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 8
- 230000035622 drinking Effects 0.000 abstract description 3
- 238000004393 prognosis Methods 0.000 abstract description 3
- 230000005764 inhibitory process Effects 0.000 abstract description 2
- 238000000034 method Methods 0.000 abstract description 2
- 210000004881 tumor cell Anatomy 0.000 abstract 1
- 238000002512 chemotherapy Methods 0.000 description 9
- 201000011510 cancer Diseases 0.000 description 8
- 210000002784 stomach Anatomy 0.000 description 7
- 238000002271 resection Methods 0.000 description 5
- 238000001959 radiotherapy Methods 0.000 description 4
- 210000000952 spleen Anatomy 0.000 description 4
- 230000000259 anti-tumor effect Effects 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 238000005538 encapsulation Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
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- 201000009030 Carcinoma Diseases 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000004064 dysfunction Effects 0.000 description 2
- 230000029058 respiratory gaseous exchange Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000001356 surgical procedure Methods 0.000 description 2
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000237852 Mollusca Species 0.000 description 1
- 208000005718 Stomach Neoplasms Diseases 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 206010016256 fatigue Diseases 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
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- 210000003734 kidney Anatomy 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/66—Proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention belongs to the technical field of health care products, and provides tumor postoperation healthy drink in order to give fully play to the inhibition effects of scallop glycoprotein I and scallop glycoprotein II on tumor cells. The tumor postoperation healthy drink is characterized by being prepared by adding the scallop glycoprotein I, the scallop glycoprotein II and granulated sugar to a traditional Chinese medicine extracting solution, wherein the weight of the traditional Chinese medicine extracting solution is used as the benchmark, the scallop glycoprotein I accounts for is 12-15wt%, the scallop glycoprotein II accounts for 15-20wt%, and the granulated sugar accounts for 8-12wt%. The tumor postoperation healthy drink is scientific in formula, has relatively mouth feel and can give fully play to effects in the prognosis health care process of tumor patients suffering tumor excision. The drinking of lots of tumor patients proves that the effective rate of the tumor postoperation healthy drink reaches above 85%.
Description
Technical field
The invention belongs to health product technology field, be specifically related to a kind of tumor postoperation healthy drink utilizing scallop zymolyte to prepare.
Background technology
At present; early metaphase tumour adopts surgery excision to be first-selected method; chemotherapy after tumor resection, radiotherapy are the effective means that control cancer cell spreads further; but although radiotherapy, chemotherapy have stronger lethal effect to cancer cell; but " fail to differentiate between the enemy and ourselves ", while fighting the enemy, also greatly compromise the normal function of human body.After local tumor excision simultaneously, body is in insufficiency of vital energy and blood, the state of dysfunction of spleen and stomach, the physical qualification of the chemotherapy now adopted, a lot of patient of radiotherapy means can not bear, therefore causes that chemotherapy appears at present a lot of patient, the radiotherapy time more grows up situation poorer phenomenon occurs.
Along with the development of life science, the Chinese traditional medicine biology activity of glycoprotein is constantly revealed, and wherein in sea mollusk, the remarkable active anticancer of glycoprotein causes the concern of medical circle.The article " chemical composition of scallop glycoprotein and the research of antitumor activity " that the Gu Qianqun etc. stepped on " Chinese Sea medicine " magazine the 3rd periodical in 1998 delivers, explain in detail in this article and how to have adopted enzyme solution to extract scallop glycoprotein I(GCFI from scallop) and scallop glycoprotein II(GCFII), and by small white mouse to scallop glycoprotein I(GCFI) and scallop glycoprotein II(GCFII) antitumor activity measure, result shows, scallop glycoprotein I(GCFI) and scallop glycoprotein II(GCFII) to mouse transplantability S180 tumour, there is more significant inhibitory action.But lost greatly by aforementioned known tumour patient its qi and blood after tumor resection, dysfunction of spleen and stomach, if merely utilize scallop glycoprotein I and scallop glycoprotein II to carry out tumor carcinoma cells suppression to patient, because its effect of weakness of health built-in function can not effectively be played, the problem therefore how utilizing the suppression effect giving full play to scallop glycoprotein to become art technology researcher urgently to study.
Summary of the invention
For giving full play to scallop glycoprotein I and scallop glycoprotein II suppression effect to tumor carcinoma cells, the present invention provides a kind of tumor postoperation healthy drink by above-mentioned glycoprotein and traditional Chinese medical extract being carried out coordinating.
The technical solution used in the present invention is: a kind of tumor postoperation healthy drink utilizing enzymolysis and extraction scallop glycoprotein to prepare, it is characterized in that, this health beverages in Chinese medicine extract, adds scallop glycoprotein I, scallop glycoprotein II and granulated sugar make, wherein with Chinese medicine extract weight for benchmark, the weight accounting of scallop glycoprotein I is that the weight accounting of 12-15%, scallop glycoprotein II is 15-20%, the weight accounting of granulated sugar is 8-12%, described scallop glycoprotein I, the preparation method of scallop glycoprotein II is, get fresh Leftover From The Scallop Chlamys Farreri to add 10 times amount hot water and boil 1h, filter cleaner, Powdered crude extract is obtained after filtrate spraying dry, preserve at 4 DEG C, crude extract suitable quantity of water is dissolved, inject bag filter to dialyse, dislysate is separated by DEAE-cellulose column, collect eluent, after concentrated, again by Sephadex G-100 column chromatography purification, freeze drying obtains scallop glycoprotein I (GCF I), by water-soluble for GCF I, 37 DEG C of water-bath mixed enzymolysis 10h, 80 DEG C of heating removing protein, filtrate uses 95% alcohol settling, collecting precipitation, freeze drying obtains scallop glycoprotein II (GCF II).
Above-mentioned Chinese medicine extract adopts the base stock Chinese medicine of following weight proportion to extract its juice through the mode that tradition decocts Chinese medicine to obtain after filtration, pawpaw 12-15 part, bighead atractylodes rhizome 9-12 part, Radix Astragali 9-12 part, Radix Angelicae Sinensis 6-9 part, Radix Glycyrrhizae 3-6 part, date 6-9 part.
The drinking way of this health beverages is drink for three times morning, noon and afternoon every day, and the amount of drinking is at 100-150ml at every turn.
In the health beverages that this invention provides, the meticulous collocation that its Chinese medicine extract loses greatly for tumour patient its qi and blood after tumor resection, taste imbalance situation carries out material medicine, it selects pawpaw, the bighead atractylodes rhizome, Radix Glycyrrhizae, date in order to strengthening the spleen and stomach, the function of motion burnt in reinforcement, middle Jiao is strong transports then coordination between the heart and kidney, extreme misery is copulated, as flat people.In medicine, in addition the first tonifying Qi medicine Radix Astragali is in order to supplementary health yang-energy again, and in addition a small amount of Radix Angelicae Sinensis, in order to tonifying yin liquid, can play the effect from cloudy yang-tonifying.Negative and positive must be mended, the strong fortune of middle Jiao, and human internal environment is improved, and the scallop glycoprotein I now in health beverages and scallop glycoprotein II can fully be absorbed by the body, and gives full play to effect of its inhibition cancer cell.This health beverage formulation science, mouthfeel is better, and can give full play to effect in the prognosis health care of tumour patient after tumor resection, through massive tumor, patient drinks, and through statistics, it is efficient reaches more than 85%.
Detailed description of the invention
Below in conjunction with embodiment, the present invention will be further described.
Being extracted in the article " chemical composition of scallop glycoprotein and the research of antitumor activity " that Gu Qianqun etc. delivers of scallop glycoprotein I and scallop glycoprotein II is mentioned in detail, its preparation method is: described scallop glycoprotein I, the preparation method of scallop glycoprotein II is, get fresh Leftover From The Scallop Chlamys Farreri to add 10 times amount hot water and boil 1h, filter cleaner, Powdered crude extract is obtained after filtrate spraying dry, preserve at 4 DEG C, crude extract suitable quantity of water is dissolved, inject bag filter to dialyse, dislysate is separated by DEAE-cellulose column, collect eluent, after concentrated, again by Sephadex G-100 column chromatography purification, freeze drying obtains scallop glycoprotein I (GCF I), by water-soluble for GCF I, 37 DEG C of water-bath mixed enzymolysis 10h, 80 DEG C of heating removing protein, filtrate uses 95% alcohol settling, collecting precipitation, freeze drying obtains scallop glycoprotein II (GCF II).
Get Chinese medicinal pawpaw 12-15 part, bighead atractylodes rhizome 9-12 part, Radix Astragali 9-12 part, Radix Angelicae Sinensis 6-9 part, Radix Glycyrrhizae 3-6 part, date 6-9 part, the mode traditionally decocting Chinese medicine is extracted its juice and is got its limpid concoction through membrane filtration.The weight portion of said medicine adjusts according to patient profiles in above-mentioned scope, weakness of the spleen and the stomach person, and the bighead atractylodes rhizome, pawpaw amount are large, when normalized volume is little; The deficiency of yang person of breathing hard increases Radix Astragali consumption, the deficiency of Yin thirsty increasing Radix Angelicae Sinensis consumption.
Embodiment 1
The tumor postoperation healthy drink utilizing enzymolysis and extraction scallop glycoprotein to prepare, gets above-mentioned 500g Chinese medicine juice, and then adds scallop glycoprotein I60g, scallop glycoprotein II100g, granulated sugar 40g in Chinese medicine juice, makes through sterilization, encapsulation.
Embodiment 2
The tumor postoperation healthy drink utilizing enzymolysis and extraction scallop glycoprotein to prepare, gets above-mentioned 500g Chinese medicine juice, and then adds scallop glycoprotein I75g, scallop glycoprotein II75g, granulated sugar 60g in Chinese medicine juice, makes through sterilization, encapsulation.
Embodiment 3
The tumor postoperation healthy drink utilizing enzymolysis and extraction scallop glycoprotein to prepare, gets above-mentioned 500g Chinese medicine juice, and then adds scallop glycoprotein I65g, scallop glycoprotein II85g, granulated sugar 50g in Chinese medicine juice, makes through sterilization, encapsulation.
Model case
Case 1
Lee, 60 years old, man, the stomach that gets a cancer of the stomach the year before excision 2/3, postoperative, lassitude, order set hypologia, and body is heavily liked sleeping, distastes for food.Check after February, find that stomach has new cancer cell to occur, doctor advised carries out surgery excision again, does not agree to, while using chemotherapy instead, this takes health beverages provided by the invention.After taking 6 months continuously, again check, have no cancer cell, continue to take so far, dietary amount continues to increase, and spirit takes a turn for the better, after meal can suitably stroll activity, and check January before, stomach has no canceration.
Case 2
Wei, female, 42 years old, was diagnosed as pernicious fibroid and excises before half a year, and the one month inner edge after excision is taken medicine limit chemotherapy, but complexion is bluish white without god, yellowish fur, do not feel like eating, vexedly breathed hard.Take health beverages of the present invention so far after chemotherapy treatment terminates, have rosy cheeks, diet is normal, vexed symptom of breathing hard disappears, hospital's further consultation, has no new canceration and occurs.
Case 3
Temperature certain, man, 65 years old, before 3 years due to thyroid gland malignant tumor excision, after end of chemotherapy, long-term self-induction is uncomfortable in chest breathes hard, do not feel like eating, and vexed, diarrhoea, takes multi-medicament invalid, before 1 year, check finds new cancer cell, but patient no longer coordinates and carries out chemotherapy, adheres to developing as one pleases.By household persuade with health beverages of the present invention so far, conscious health is greatly improved, before January hospital check, cancer cell disappears, still holds at present and takes.
The tumor postoperation healthy drink utilizing enzymolysis and extraction scallop glycoprotein to prepare provided by the invention taking through 92 routine tumor resection patients, Time of Administration more than 6 months, 83 examples that self-induction health takes a turn for the better, unchanged 7 examples, 2 examples feel that health continues to worsen.Through hospital's check, 79 examples do not reproduce new canceration, and new canceration appears in 3 examples, and 10 examples are apprised of and are returned to one's perfect health.Through above-mentioned statistics, the prognosis of this health beverages to tumour patient keeps healthy, and it efficiently reaches more than 85%.
Claims (1)
1. the tumor postoperation healthy drink utilizing enzymolysis and extraction scallop glycoprotein to prepare, it is characterized in that, this health beverages in Chinese medicine extract, adds scallop glycoprotein I, scallop glycoprotein II and granulated sugar make, wherein with Chinese medicine extract weight for benchmark, the weight accounting of scallop glycoprotein I is that the weight accounting of 12-15%, scallop glycoprotein II is 15-20%, the weight accounting of granulated sugar is 8-12%, described scallop glycoprotein I, the preparation method of scallop glycoprotein II is, get fresh Leftover From The Scallop Chlamys Farreri to add 10 times amount hot water and boil 1h, filter cleaner, Powdered crude extract is obtained after filtrate spraying dry, preserve at 4 DEG C, crude extract suitable quantity of water is dissolved, inject bag filter to dialyse, dislysate is separated by DEAE-cellulose column, collect eluent, after concentrated, again by Sephadex G-100 column chromatography purification, freeze drying obtains scallop glycoprotein I (GCF I), by water-soluble for GCF I, 37 DEG C of water-bath mixed enzymolysis 10h, 80 DEG C of heating removing protein, filtrate uses 95% alcohol settling, collecting precipitation, freeze drying obtains scallop glycoprotein II (GCF II), described Chinese medicine extract adopts the base stock Chinese medicine of following weight proportion to extract its juice through the mode that tradition decocts Chinese medicine to obtain after filtration, pawpaw 12-15 part, bighead atractylodes rhizome 9-12 part, Radix Astragali 9-12 part, Radix Angelicae Sinensis 6-9 part, Radix Glycyrrhizae 3-6 part, date 6-9 part.
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| CN201310469142.8A CN103535825B (en) | 2013-10-10 | 2013-10-10 | Tumor postoperation healthy drink prepared by utilizing enzymolysis for extracting scallop glycoprotein |
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| CN201310469142.8A CN103535825B (en) | 2013-10-10 | 2013-10-10 | Tumor postoperation healthy drink prepared by utilizing enzymolysis for extracting scallop glycoprotein |
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| CN103535825A CN103535825A (en) | 2014-01-29 |
| CN103535825B true CN103535825B (en) | 2015-05-20 |
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| CN201310469142.8A Expired - Fee Related CN103535825B (en) | 2013-10-10 | 2013-10-10 | Tumor postoperation healthy drink prepared by utilizing enzymolysis for extracting scallop glycoprotein |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101601487A (en) * | 2009-07-09 | 2009-12-16 | 山东好当家海洋发展股份有限公司 | A kind of shellfish protein beverage and preparation method thereof |
| CN102283379A (en) * | 2011-07-13 | 2011-12-21 | 刘树平 | Health-care product and production process thereof |
| CN102293995A (en) * | 2011-07-14 | 2011-12-28 | 北京汉典中西药研究开发中心 | Spleen-invigorating the qi-replenishing particle and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007282506A (en) * | 2006-04-12 | 2007-11-01 | Kojo:Kk | Calcium-supplying beverage and method for producing the same |
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2013
- 2013-10-10 CN CN201310469142.8A patent/CN103535825B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101601487A (en) * | 2009-07-09 | 2009-12-16 | 山东好当家海洋发展股份有限公司 | A kind of shellfish protein beverage and preparation method thereof |
| CN102283379A (en) * | 2011-07-13 | 2011-12-21 | 刘树平 | Health-care product and production process thereof |
| CN102293995A (en) * | 2011-07-14 | 2011-12-28 | 北京汉典中西药研究开发中心 | Spleen-invigorating the qi-replenishing particle and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 顾谦群,等.扇贝糖蛋白的化学组成与抗肿瘤活性的研究.《中国海洋药物》.1998,(第3期),第23-26页. * |
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