CN103535526A - Feed additive for improving fat utilization rate - Google Patents
Feed additive for improving fat utilization rate Download PDFInfo
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- CN103535526A CN103535526A CN201310497576.9A CN201310497576A CN103535526A CN 103535526 A CN103535526 A CN 103535526A CN 201310497576 A CN201310497576 A CN 201310497576A CN 103535526 A CN103535526 A CN 103535526A
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- feed
- utilization rate
- emulsifying agent
- feed addictive
- fat
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- 239000003674 animal food additive Substances 0.000 title abstract 3
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 36
- 230000000694 effects Effects 0.000 claims abstract description 11
- 206010018910 Haemolysis Diseases 0.000 claims abstract description 7
- 230000008588 hemolysis Effects 0.000 claims abstract description 7
- 239000000654 additive Substances 0.000 claims abstract description 6
- 230000000996 additive effect Effects 0.000 claims abstract description 6
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 26
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 18
- 229940083466 soybean lecithin Drugs 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 14
- 238000000605 extraction Methods 0.000 claims description 12
- 102100037611 Lysophospholipase Human genes 0.000 claims description 9
- 108010058864 Phospholipases A2 Proteins 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 8
- 239000000377 silicon dioxide Substances 0.000 claims description 7
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 230000006837 decompression Effects 0.000 claims description 6
- 238000001704 evaporation Methods 0.000 claims description 6
- 230000000937 inactivator Effects 0.000 claims description 6
- ASWBNKHCZGQVJV-UHFFFAOYSA-N (3-hexadecanoyloxy-2-hydroxypropyl) 2-(trimethylazaniumyl)ethyl phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(O)COP([O-])(=O)OCC[N+](C)(C)C ASWBNKHCZGQVJV-UHFFFAOYSA-N 0.000 claims description 5
- 239000004519 grease Substances 0.000 abstract description 11
- 241001465754 Metazoa Species 0.000 abstract description 6
- 239000002994 raw material Substances 0.000 abstract description 3
- 231100000252 nontoxic Toxicity 0.000 abstract description 2
- 230000003000 nontoxic effect Effects 0.000 abstract description 2
- 230000003647 oxidation Effects 0.000 abstract description 2
- 238000007254 oxidation reaction Methods 0.000 abstract description 2
- PHIQHXFUZVPYII-ZCFIWIBFSA-N (R)-carnitine Chemical compound C[N+](C)(C)C[C@H](O)CC([O-])=O PHIQHXFUZVPYII-ZCFIWIBFSA-N 0.000 abstract 2
- 238000010521 absorption reaction Methods 0.000 abstract 2
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 abstract 1
- 244000068988 Glycine max Species 0.000 abstract 1
- 235000010469 Glycine max Nutrition 0.000 abstract 1
- 239000004480 active ingredient Substances 0.000 abstract 1
- 238000000354 decomposition reaction Methods 0.000 abstract 1
- 230000029087 digestion Effects 0.000 abstract 1
- 230000001804 emulsifying effect Effects 0.000 abstract 1
- 239000000787 lecithin Substances 0.000 abstract 1
- 229940067606 lecithin Drugs 0.000 abstract 1
- 235000010445 lecithin Nutrition 0.000 abstract 1
- 230000002195 synergetic effect Effects 0.000 abstract 1
- 235000019197 fats Nutrition 0.000 description 15
- 206010012735 Diarrhoea Diseases 0.000 description 11
- 244000144972 livestock Species 0.000 description 6
- 238000002156 mixing Methods 0.000 description 6
- 244000144977 poultry Species 0.000 description 6
- 235000014590 basal diet Nutrition 0.000 description 5
- 230000002354 daily effect Effects 0.000 description 5
- 235000021050 feed intake Nutrition 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- 241000282898 Sus scrofa Species 0.000 description 3
- 240000008042 Zea mays Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 3
- 235000005822 corn Nutrition 0.000 description 3
- 239000007791 liquid phase Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000013589 supplement Substances 0.000 description 3
- 241000282887 Suidae Species 0.000 description 2
- 238000004945 emulsification Methods 0.000 description 2
- -1 fatty acid ester Chemical class 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- HSINOMROUCMIEA-FGVHQWLLSA-N (2s,4r)-4-[(3r,5s,6r,7r,8s,9s,10s,13r,14s,17r)-6-ethyl-3,7-dihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1h-cyclopenta[a]phenanthren-17-yl]-2-methylpentanoic acid Chemical class C([C@@]12C)C[C@@H](O)C[C@H]1[C@@H](CC)[C@@H](O)[C@@H]1[C@@H]2CC[C@]2(C)[C@@H]([C@H](C)C[C@H](C)C(O)=O)CC[C@H]21 HSINOMROUCMIEA-FGVHQWLLSA-N 0.000 description 1
- 235000019737 Animal fat Nutrition 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 235000019730 animal feed additive Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003833 bile salt Substances 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
Landscapes
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a feed additive for improving the fat utilization rate. The additive consists of an emulsifier and L-carnitine at a weight ratio of (2.67-6.67):1, wherein the active ingredient of the emulsifier is hemolysis soya bean lecithin. In the feed additive disclosed by the invention, the emulsifier can promote the fat digestion and absorption by animals, and the L-carnitine can accelerate the fat oxidation and decomposition; by combining the two, a synergistic promotion effect is realized in terms of fat absorption and utilization by animals, growth performance and the like, the emulsifying effect on the feed fat is good, the growth promotion effect is obvious, the amount of grease added into the feed can be directly reduced, and the using cost of a feed is lowered; and moreover, the raw materials are all nontoxic and convenient to use, and the biosafety is high.
Description
Technical field
The invention belongs to animal feed additive field, relate to a kind of feed addictive that improves fat utilization rate.
Background technology
Along with the development of animal husbandry, in pursuing the process of the high effect of cultivation, in order to accelerate the speed of growth of livestock and poultry, reduce feedstuff-meat ratio, in feed, use the situation of grease more and more general.By rational use grease, the production performance of livestock and poultry improves greatly, and the speed of growth is accelerated, and at present in order to improve the speed of growth and the production performance of livestock and poultry, usually adds in a large number grease.In the daily ration in broiler chicken material later stage, the adding proportion of grease usually surpasses 3%, grease in lactating swine diets also can reach more than 3%, in such adding proportion situation, the grease adding in feed usually can not reach our desired value, this is mainly because the needed bile salt amount of a high proportion of grease has substantially exceeded the amount that can secrete in livestock and poultry body in daily ration, so the emulsification of grease is not thorough, do not have the grease of emulsification usually to cause the diarrhoea of livestock and poultry, thereby cause the loss of Production of Livestock and Poultry.In order to improve animal fat utilization rate interpolation emulsifying agent, be important means, the emulsifying agent that the existing stage is used has tens of kinds, but for mainly containing on feed industry: phospholipid, fatty acid ester, glycoside esters, bile acid salt emulsifying agent etc., these exogenous emulsifying agents have certain effect, but do not reach satisfied effect.
Summary of the invention
The object of the present invention is to provide a kind of feed addictive that improves fat utilization rate.
The object of the present invention is to provide a kind of feed that contains above-mentioned feed addictive.
The technical solution used in the present invention is:
Improve a feed addictive for fat utilization rate, this additive is comprised of emulsifying agent and VBT.
Further, the weight ratio of described emulsifying agent and VBT is (2.67～6.67) ︰ 1.
Further, the weight ratio of described emulsifying agent and VBT is 5 ︰ 1.
Further described emulsifying agent, its preparation method comprises the following steps:
1) commercially available soybean lecithin ligroin extraction, water and phospholipase A2 three are mixed, at 38～55 ℃, react completely and generate haemolysis soybean lecithin;
2) by reaction system 60～100 ℃ of inactivator activity, then vacuumize decompression, evaporating water;
3) toward step 2) in add silica to make the mass content of acetone insoluble matter to be 25～35%, fully to mix, be emulsifying agent, wherein, the mass content of lysolecithin is 1～1.5%.
Further, in step 1), commercially available soybean lecithin ligroin extraction, water and phospholipase A2 three's mass percent is respectively 95～98.9%, 1～4%, 0.1～1.0%.
Further, mass content >=55% of acetone insoluble matter in described commercially available soybean lecithin ligroin extraction.
Further, the addition of described feed addictive is 0.03～0.08% of feed weight.
Further, the addition of described feed addictive is 0.06% of feed weight.
The invention has the beneficial effects as follows:
In feed addictive of the present invention, emulsifying agent can promote animal digesting and assimilating fat, VBT can accelerate fat oxidation decompose, being combined with of the two absorbs the collaborative facilitations of aspect performance such as fat and growth performance animal, can directly reduce the addition of grease in feed, reduce the use cost of feed.
Raw material of the present invention is nontoxic, easy to use, and biological safety is high, and raw material is easy to get, price is low; Additive of the present invention is made an addition in animal feed, can significantly improve the utilization ratio of nutriment in feed, obvious to the emulsify well of feed fat, growth-promoting effect.
The specific embodiment
A preparation method for the feed addictive of fat utilization rate, comprises the following steps:
1) preparation of emulsifying agent:
toward step
) in add silica to make the mass content of acetone insoluble matter to be 25～35%, fully to mix, be emulsifying agent, wherein, the mass content of lysolecithin is 1.0～1.5%;
2) by 1) in emulsifying agent and VBT in mass ratio for (2.67～6.67) ︰ 1 at room temperature stir and fully mix, and obtain the feed addictive of raising fat utilization rate.
Preferably, step
) in commercially available soybean lecithin ligroin extraction, water and phospholipase A2 three 98.9%, 1%, 0.1% mix by mass percentage respectively.
Step
described in commercially available soybean lecithin ligroin extraction acetone insoluble matter mass content be preferably>=55%.
Step
in add silica to make the mass content of acetone insoluble matter be preferably 25%.
Step
described in the mass content of lysolecithin be preferably 1.0% of emulsifying agent.
Step 2) described emulsifying agent and VBT fully mixes by preferred mass ratio 5 ︰ 1.
Below in conjunction with specific embodiment, the invention will be further described, but be not limited to this.
embodiment 1:
1) emulsifying agent preparation: get commercially available soybean lecithin ligroin extraction 1000g(wherein acetone insoluble matter content be 55%), toward wherein adding 1mL phospholipase A2, (enzyme lives >=10,000UN/mL) with 10g water, at 40 ℃ of reaction 8h, then at the active 10min of 80 ℃ of inactivators, then vacuumize decompression, evaporating water, add again silica 1 200g to make acetone insoluble matter content reduce to 25%, at room temperature be uniformly mixed 4h, mixing speed 150rpm, obtain emulsifying agent, by efficient liquid phase chromatographic analysis, the mass content of haemolysis soybean lecithin is 1%.
2) get 1) middle emulsifying agent 500g, add 100g VBT, at room temperature stir 4h, mixing speed 150rpm, obtains the feed addictive that improves fat utilization rate.
embodiment 2:
1) emulsifying agent preparation: get commercially available soybean lecithin ligroin extraction 1000g(wherein acetone insoluble matter content be 55%), toward wherein adding 1mL phospholipase A2, (enzyme lives >=10,000UN/mL) with 30g water, at 55 ℃ of reaction 8h, then at the active 10min of 80 ℃ of inactivators, then vacuumize decompression, evaporating water, add again silica 1 000g to make acetone insoluble matter content reduce to 27.5%, at room temperature be uniformly mixed 4h, mixing speed 150rpm, obtain emulsifying agent, by efficient liquid phase chromatographic analysis, the mass content of haemolysis soybean lecithin is 1.1%.
2) get 1) middle emulsifying agent 667g, add 100g VBT, at room temperature stir 4h, mixing speed 150rpm.
embodiment 3:
1) emulsifying agent preparation: get commercially available soybean lecithin ligroin extraction 1000g(wherein acetone insoluble matter content be 55%), toward wherein adding 1.5mL phospholipase A2, (enzyme lives >=10,000UN/mL) with 40g water, at 38 ℃ of reaction 8h, then at the active 10min of 60 ℃ of inactivators, then vacuumize decompression, evaporating water, add again silica 600g to make acetone insoluble matter content reduce to 34%, at room temperature be uniformly mixed 4h, mixing speed 150rpm, obtain emulsifying agent, by efficient liquid phase chromatographic analysis, the mass content of haemolysis soybean lecithin is 1.3%.
2) get 1) middle emulsifying agent 267g, add 100g VBT, at room temperature stir 3h, mixing speed 120rpm.
Below the feed addictive of preparing in embodiment is made to further effect detection.
Select 120 of 30 age in days weanling pigs, body weight is 8.52 ± 0.76 kg, is divided at random 4 groups, every group of 5 repetitions, and each repeats 6 pigs.
1 basal diet of feeding of control group;
Control group 2 feed basal diet and VBT (VBT be total feed heavy 0.01%);
Control group 3 feed basal diet and emulsifying agent (emulsifying agent be total feed heavy 0.05%);
1 group of embodiment feed basal diet and embodiment 1 preparation feed addictive (feed addictive be total feed heavy 0.06%);
Each composition of the daily ration of feeding in each group and additive and content thereof refer to following table 1.Wherein, it is 3% that the fat of control group 1 basal diet adds quality, control group 2 fat adds quality to be 2.9% and to supplement 0.1% corn, and the fat of control group 3 adds quality to be 2.5% and to supplement 0.5% corn, and the fat that embodiment is 1 group adds quality to be 2.1% and to supplement 0.9% corn.
Feed ingredient and the mass percent thereof of each group of table 1
In feeding process, record the feed intake of every day, statistics diarrhoea situation etc., raised after 14 days, and the end that weighs pig is heavy, and calculates average feed intake (g/d), average daily gain g, feedstuff-meat ratio, the indexs such as diarrhea rate (in Table 2).
From the data of table 2, can find out that each group is heavy in initial weight, end, in the index such as feed intake and daily gain without significant difference (
p>0.05).Wherein, from absolute figure, 1 group of feed intake of embodiment is the highest, reaches 342.8 g/d.
From material anharmonic ratio, the feedstuff-meat ratio of control group 1 is 1.39, and control group 2 is 1.27, and control group 3 is that 1 group of 1.25, embodiment is 1.15, wherein 1 group of embodiment and control group 1 have significant difference (
p<0.05), 1,2 and 3 of control groups without significant difference (
p>0.05), visible, although the fatty addition of 1 group of embodiment is minimum, feedstuff-meat ratio is minimum, is more conducive to piglet to fatty trans-utilization.
From diarrhea rate index, the diarrhea rate of control group 1 is 7.6%, and 1 group of control group 2,3 and embodiment have all reduced diarrhea rate, is respectively 1 group of diarrhea rate of 6.0%, 5.8%, 5.5%, embodiment minimum.
Table 2 adds VBT and the impact of lysolecithin on Growth Performance of Weaning Piglets and diarrhea rate
Note:
*represent
p<0.05.
In sum, comparing with control group 1, though add separately VBT and emulsifying agent, can reduce the feedstuff-meat ratio of piglet and diarrhea rate etc., there is not significant difference in index of correlation.And add the additive being formed by VBT and emulsifying agent, embodiment is 1 group, can significantly reduce feedstuff-meat ratio and the diarrhea rate of weanling pig.The common use of visible VBT and floating agent has unexpected synergy, and feed addictive of the present invention can significantly improve piglet to fatty utilization rate, improves the comprehensive growth performance of piglet integral body, reduces diarrhea rate, and growth-promoting effect is also obvious.
Claims (7)
1. a feed addictive that improves fat utilization rate, is characterized in that: this additive is comprised of emulsifying agent and VBT.
2. a kind of feed addictive that improves fat utilization rate according to claim 1, is characterized in that: the weight ratio of described emulsifying agent and VBT is (2.67～6.67) ︰ 1.
3. a kind of feed addictive that improves fat utilization rate according to claim 1, is characterized in that: the weight ratio of described emulsifying agent and VBT is 5 ︰ 1.
4. a kind of feed addictive that improves fat utilization rate according to claim 1, is characterized in that: the preparation method of described emulsifying agent comprises the following steps:
1) commercially available soybean lecithin ligroin extraction, water and phospholipase A2 three are mixed, at 38～55 ℃, react completely and generate haemolysis soybean lecithin;
2) by reaction system 60～100 ℃ of inactivator activity, then vacuumize decompression, evaporating water;
3) toward step 2) in add silica to make the mass content of acetone insoluble matter to be 25～35%, fully to mix, be emulsifying agent, wherein, the mass content of lysolecithin is 1～1.5%.
5. a kind of feed addictive that improves fat utilization rate according to claim 4, is characterized in that: in step 1), commercially available soybean lecithin ligroin extraction, water and phospholipase A2 three's mass percent is respectively 95～98.9%, 1～4%, 0.1～1.0%.
6. according to a kind of feed addictive that improves fat utilization rate described in claim 4 or 5, it is characterized in that: mass content >=55% of acetone insoluble matter in described commercially available soybean lecithin ligroin extraction.
7. a feed, is characterized in that: the feed addictive that contains raising fat utilization rate claimed in claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310497576.9A CN103535526B (en) | 2013-10-22 | 2013-10-22 | Feed additive for improving fat utilization rate |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310497576.9A CN103535526B (en) | 2013-10-22 | 2013-10-22 | Feed additive for improving fat utilization rate |
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| Publication Number | Publication Date |
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| CN103535526A true CN103535526A (en) | 2014-01-29 |
| CN103535526B CN103535526B (en) | 2015-04-22 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109805194A (en) * | 2019-03-28 | 2019-05-28 | 东北农业大学 | A kind of compound emulsifying agent of animal feed, application method and application |
| CN113072601A (en) * | 2021-03-30 | 2021-07-06 | 固安君德同创生物工程有限公司 | Preparation method and application of functional oligosaccharide glycoside ester |
| CN114698734A (en) * | 2022-05-19 | 2022-07-05 | 广东海纳川生物科技股份有限公司 | Feeding lysolecithin and preparation method thereof, and feeding emulsifier and preparation method thereof |
| CN117837676A (en) * | 2023-12-27 | 2024-04-09 | 江苏四新界面剂科技有限公司 | Preparation method of probiotics emulsifier for feed production |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1197115A (en) * | 1997-04-08 | 1998-10-28 | 辻制油株式会社 | Process for manufacturing vegetable lysolecithins |
| KR20110131607A (en) * | 2010-05-31 | 2011-12-07 | 주식회사 카파아이엔티 | Powder-type whipping cream composition for beverages and method for making the same |
| CN103131736A (en) * | 2013-02-25 | 2013-06-05 | 上海艾韦特医药科技有限公司 | Preparation and application of high-purity lysophosphatidylcholine |
-
2013
- 2013-10-22 CN CN201310497576.9A patent/CN103535526B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1197115A (en) * | 1997-04-08 | 1998-10-28 | 辻制油株式会社 | Process for manufacturing vegetable lysolecithins |
| KR20110131607A (en) * | 2010-05-31 | 2011-12-07 | 주식회사 카파아이엔티 | Powder-type whipping cream composition for beverages and method for making the same |
| CN103131736A (en) * | 2013-02-25 | 2013-06-05 | 上海艾韦特医药科技有限公司 | Preparation and application of high-purity lysophosphatidylcholine |
Non-Patent Citations (1)
| Title |
|---|
| 柘丽，等: "饲粮添加乳化剂与L-肉碱对肉鸡生长性能和血脂指标的影响", 《动物营养学报》, vol. 24, no. 9, 30 September 2012 (2012-09-30), pages 1755 - 1763 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109805194A (en) * | 2019-03-28 | 2019-05-28 | 东北农业大学 | A kind of compound emulsifying agent of animal feed, application method and application |
| CN113072601A (en) * | 2021-03-30 | 2021-07-06 | 固安君德同创生物工程有限公司 | Preparation method and application of functional oligosaccharide glycoside ester |
| CN114698734A (en) * | 2022-05-19 | 2022-07-05 | 广东海纳川生物科技股份有限公司 | Feeding lysolecithin and preparation method thereof, and feeding emulsifier and preparation method thereof |
| CN117837676A (en) * | 2023-12-27 | 2024-04-09 | 江苏四新界面剂科技有限公司 | Preparation method of probiotics emulsifier for feed production |
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| CN103535526B (en) | 2015-04-22 |
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Denomination of invention: Feed additive for improving fat utilization rate Effective date of registration: 20180330 Granted publication date: 20150422 Pledgee: China Co truction Bank Corp Guangzhou economic and Technological Development Zone sub branch Pledgor: Guangzhou Wisdom Bio-Technology Co., Ltd. Registration number: 2018440000073 |
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Denomination of invention: Feed additive for improving fat utilization rate Effective date of registration: 20200107 Granted publication date: 20150422 Pledgee: Bank of China, Limited by Share Ltd, Guangzhou, Panyu branch Pledgor: Guangzhou Wisdom Bio-Technology Co., Ltd. Registration number: Y2020440000002 |
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