CN114698734A - Feeding lysolecithin and preparation method thereof, and feeding emulsifier and preparation method thereof - Google Patents
Feeding lysolecithin and preparation method thereof, and feeding emulsifier and preparation method thereof Download PDFInfo
- Publication number
- CN114698734A CN114698734A CN202210545605.3A CN202210545605A CN114698734A CN 114698734 A CN114698734 A CN 114698734A CN 202210545605 A CN202210545605 A CN 202210545605A CN 114698734 A CN114698734 A CN 114698734A
- Authority
- CN
- China
- Prior art keywords
- feeding
- lysolecithin
- parts
- enzymolysis
- emulsifier
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- ASWBNKHCZGQVJV-UHFFFAOYSA-N (3-hexadecanoyloxy-2-hydroxypropyl) 2-(trimethylazaniumyl)ethyl phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OCC(O)COP([O-])(=O)OCC[N+](C)(C)C ASWBNKHCZGQVJV-UHFFFAOYSA-N 0.000 title claims abstract description 113
- 239000003995 emulsifying agent Substances 0.000 title claims abstract description 76
- 238000002360 preparation method Methods 0.000 title claims abstract description 30
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims abstract description 42
- 229940083466 soybean lecithin Drugs 0.000 claims abstract description 41
- ZIIUUSVHCHPIQD-UHFFFAOYSA-N 2,4,6-trimethyl-N-[3-(trifluoromethyl)phenyl]benzenesulfonamide Chemical compound CC1=CC(C)=CC(C)=C1S(=O)(=O)NC1=CC=CC(C(F)(F)F)=C1 ZIIUUSVHCHPIQD-UHFFFAOYSA-N 0.000 claims abstract description 20
- 102000015439 Phospholipases Human genes 0.000 claims abstract description 20
- 108010064785 Phospholipases Proteins 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000010438 heat treatment Methods 0.000 claims abstract description 16
- 102000004190 Enzymes Human genes 0.000 claims abstract description 15
- 108090000790 Enzymes Proteins 0.000 claims abstract description 15
- 239000008347 soybean phospholipid Substances 0.000 claims abstract description 10
- 239000011159 matrix material Substances 0.000 claims description 33
- 239000004480 active ingredient Substances 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 16
- 239000000758 substrate Substances 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 14
- 239000000843 powder Substances 0.000 claims description 14
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims description 12
- AZJQQNWSSLCLJN-UHFFFAOYSA-N 2-ethoxyquinoline Chemical compound C1=CC=CC2=NC(OCC)=CC=C21 AZJQQNWSSLCLJN-UHFFFAOYSA-N 0.000 claims description 10
- CZBZUDVBLSSABA-UHFFFAOYSA-N butylated hydroxyanisole Chemical compound COC1=CC=C(O)C(C(C)(C)C)=C1.COC1=CC=C(O)C=C1C(C)(C)C CZBZUDVBLSSABA-UHFFFAOYSA-N 0.000 claims description 10
- 230000009849 deactivation Effects 0.000 claims description 8
- 235000007164 Oryza sativa Nutrition 0.000 claims description 7
- 229920000136 polysorbate Polymers 0.000 claims description 7
- 235000009566 rice Nutrition 0.000 claims description 7
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 6
- BCZXFFBUYPCTSJ-UHFFFAOYSA-L Calcium propionate Chemical group [Ca+2].CCC([O-])=O.CCC([O-])=O BCZXFFBUYPCTSJ-UHFFFAOYSA-L 0.000 claims description 6
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 6
- 229930003427 Vitamin E Natural products 0.000 claims description 6
- 235000010331 calcium propionate Nutrition 0.000 claims description 6
- 239000004330 calcium propionate Substances 0.000 claims description 6
- 239000006229 carbon black Substances 0.000 claims description 6
- 210000000078 claw Anatomy 0.000 claims description 6
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims description 6
- 235000012054 meals Nutrition 0.000 claims description 6
- 235000019165 vitamin E Nutrition 0.000 claims description 6
- 229940046009 vitamin E Drugs 0.000 claims description 6
- 239000011709 vitamin E Substances 0.000 claims description 6
- 102000004882 Lipase Human genes 0.000 claims description 5
- 108090001060 Lipase Proteins 0.000 claims description 5
- 239000004367 Lipase Substances 0.000 claims description 5
- 235000019421 lipase Nutrition 0.000 claims description 5
- 102100031415 Hepatic triacylglycerol lipase Human genes 0.000 claims description 4
- 108010013563 Lipoprotein Lipase Proteins 0.000 claims description 4
- 102100037611 Lysophospholipase Human genes 0.000 claims description 4
- 108010058864 Phospholipases A2 Proteins 0.000 claims description 4
- 229910021536 Zeolite Inorganic materials 0.000 claims description 4
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 4
- 239000010459 dolomite Substances 0.000 claims description 4
- 229910000514 dolomite Inorganic materials 0.000 claims description 4
- 239000010457 zeolite Substances 0.000 claims description 4
- 108010068370 Glutens Proteins 0.000 claims description 3
- 240000008042 Zea mays Species 0.000 claims description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 3
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 3
- 239000000440 bentonite Substances 0.000 claims description 3
- 229910000278 bentonite Inorganic materials 0.000 claims description 3
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 3
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 3
- 235000005822 corn Nutrition 0.000 claims description 3
- 235000021312 gluten Nutrition 0.000 claims description 3
- 239000003112 inhibitor Substances 0.000 claims 3
- 240000007594 Oryza sativa Species 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 abstract description 10
- 238000010521 absorption reaction Methods 0.000 abstract description 8
- 230000029087 digestion Effects 0.000 abstract description 7
- 239000003674 animal food additive Substances 0.000 abstract description 6
- 244000144972 livestock Species 0.000 abstract description 6
- 230000003449 preventive effect Effects 0.000 description 15
- 229940088598 enzyme Drugs 0.000 description 11
- 230000000694 effects Effects 0.000 description 8
- 239000002994 raw material Substances 0.000 description 8
- 230000000415 inactivating effect Effects 0.000 description 6
- 241000209094 Oryza Species 0.000 description 5
- 244000144977 poultry Species 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 229940040461 lipase Drugs 0.000 description 4
- 150000003904 phospholipids Chemical class 0.000 description 4
- 229920001213 Polysorbate 20 Polymers 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 3
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 3
- 239000003833 bile salt Substances 0.000 description 2
- 229940093761 bile salts Drugs 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 235000014113 dietary fatty acids Nutrition 0.000 description 2
- 239000000194 fatty acid Substances 0.000 description 2
- 229930195729 fatty acid Natural products 0.000 description 2
- 150000004665 fatty acids Chemical class 0.000 description 2
- 239000000945 filler Substances 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- LDVVTQMJQSCDMK-UHFFFAOYSA-N 1,3-dihydroxypropan-2-yl formate Chemical compound OCC(CO)OC=O LDVVTQMJQSCDMK-UHFFFAOYSA-N 0.000 description 1
- RYCNUMLMNKHWPZ-SNVBAGLBSA-N 1-acetyl-sn-glycero-3-phosphocholine Chemical compound CC(=O)OC[C@@H](O)COP([O-])(=O)OCC[N+](C)(C)C RYCNUMLMNKHWPZ-SNVBAGLBSA-N 0.000 description 1
- 108010004103 Chylomicrons Proteins 0.000 description 1
- 102000019280 Pancreatic lipases Human genes 0.000 description 1
- 108050006759 Pancreatic lipases Proteins 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000012736 aqueous medium Substances 0.000 description 1
- OGBUMNBNEWYMNJ-UHFFFAOYSA-N batilol Chemical class CCCCCCCCCCCCCCCCCCOCC(O)CO OGBUMNBNEWYMNJ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 230000001079 digestive effect Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 235000021588 free fatty acids Nutrition 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 235000021238 nutrient digestion Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 229940116369 pancreatic lipase Drugs 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Fodder In General (AREA)
Abstract
The invention relates to the technical field of feed emulsifiers, in particular to feeding lysolecithin and a preparation method thereof, and a feeding emulsifier and a preparation method thereof. A preparation method of feeding lysolecithin comprises the following steps: step (1), putting soybean phospholipids into an enzymolysis tank for preheating; step (2), adding water; step (3), adding phospholipase; step (4), heating and maintaining the enzymolysis temperature for enzymolysis; and (5) after the enzymolysis is finished, heating to inactivate the enzyme, and obtaining the feeding lysolecithin. The preparation method of the feeding lysolecithin can improve the content of the lysolecithin in the soybean lecithin, has high enzymolysis yield of the lysolecithin, effectively reduces the production cost, can promote the digestion and absorption of fat by livestock when being used as a feed additive, and solves the problems of low enzymolysis yield of the lysolecithin in the soybean lecithin and high production cost of the lysolecithin at present.
Description
Technical Field
The invention relates to the technical field of feed emulsifiers, in particular to feeding lysolecithin and a preparation method thereof, and a feeding emulsifier and a preparation method thereof.
Background
A fundamental problem with fat digestion is that fat is not water soluble and fat digestion is carried out in an aqueous system. The first step of fat digestion is fat emulsification, where the emulsified fat enters the aqueous medium together with lipase, and this process is accomplished by bile, which has the function of activating pancreatic lipase and emulsified fat, playing an important role in nutrient digestion and absorption. Surfactants in bile salts create microdroplets or microbubbles in which monoglycerides and free fatty acids aggregate to form three-dimensional structures. These microbubbles are transported to the wall of the gastrointestinal tract and transport nutrients to the intestinal villi for absorption. These lipid substances are absorbed and then re-synthesized into triglycerides in the form of chylomicrons, and then enter the blood circulation.
The phospholipid is used as an emulsifier and can form a micro-bubble structure to assist fatty acid absorption, lysolecithin (also called lysophospholipid) is a special phospholipid and is more hydrophilic than other phospholipids, and spontaneously forms micro-bubbles together with bile salts, fatty acids and monoglyceride. Lysolecithin is used as a feed fat emulsifier, so that the cost of livestock and poultry breeding can be effectively reduced, and the quality of the feeding lysolecithin emulsifier on the current market is uneven, so that the following problems mainly exist: the enzymolysis yield of the lysolecithin in the soybean lecithin is low, the content of the lysolecithin in the feeding emulsifier is low, the production cost of the lysolecithin is high, and in addition, the components marked by the current feeding emulsifier contain the lysolecithin, but the soybean lecithin is actually simply compounded, so the popularization and the use of the lysolecithin in the feed industry are restricted.
Disclosure of Invention
The invention aims to provide a preparation method of feeding lysolecithin, which can improve the content of lysolecithin in soybean lecithin, has high enzymolysis yield of lysolecithin, effectively reduces the production cost, can promote the digestion and absorption of fat by livestock and poultry when used as a feed additive, and solves the problems of low enzymolysis yield of lysolecithin in soybean lecithin and high production cost of lysolecithin at present.
The invention also aims to provide the feeding lysolecithin, which has high content of lysolecithin and solves the problem of low enzymolysis yield of the lysolecithin in the conventional soybean lecithin.
The invention also aims to provide a feeding emulsifier, which has high content of lysolecithin, and solves the problems that the conventional feeding emulsifier is only used for simply compounding soybean lecithin, and the content of the lysolecithin in the feeding emulsifier is low, so that the feeding emulsifier is inconvenient to add and use in feeds.
The invention also aims to provide a preparation method of the feeding emulsifier, which is characterized in that the content of lysolecithin in the prepared feeding emulsifier is high, and the problem that the conventional feeding emulsifier is inconvenient to add and use in feed due to low content of lysolecithin is solved.
In order to achieve the purpose, the invention adopts the following technical scheme:
a preparation method of feeding lysolecithin comprises the following steps:
step (1), putting soybean phospholipids into an enzymolysis tank for preheating;
step (2), adding water;
adding phospholipase;
heating and keeping the enzymolysis temperature for enzymolysis;
and (5) after the enzymolysis is finished, heating to inactivate enzyme to obtain the feeding lysolecithin.
Further, in the step (1), the preheating temperature is 40-60 ℃, and the preheating time is 10-60 min;
in the step (4), the enzymolysis temperature is 50-70 ℃, and the enzymolysis time is 1.5-4 h;
in the step (5), the enzyme deactivation temperature is 90-100 ℃, and the enzyme deactivation time is 0.5-1 h.
In the step (2), the addition amount of water is 5-25% of the mass of the soybean lecithin;
in the step (3), the addition amount of phospholipase is 0.1-1.5% of the mass of the soybean phospholipids, and the phospholipase is phospholipase A1, phospholipase A2 or lipase.
Further, in the step (4), after the vitamin E is added, heating and maintaining the enzymolysis temperature for enzymolysis, wherein the addition amount of the vitamin E is 0.01-0.1% of the mass of the soybean lecithin.
The feeding lysolecithin is prepared by the preparation method of the feeding lysolecithin.
The feeding lysolecithin is used as a feeding emulsifier, and the raw materials comprise, by mass, 30.2-71 parts of an active ingredient and 47-58 parts of a matrix, wherein the active ingredient comprises 25-50 parts of feeding lysolecithin, 5-20 parts of tween, 0.1-0.5 part of butyl hydroxy anisole and 0.1-0.5 part of ethoxyquinoline;
the substrate is selected from one or a combination of two of rice bran meal, corncob powder, unite bran, defatted rice bran, corn gluten meal, white carbon black, zeolite powder, bentonite, calcium carbonate and dolomite powder.
Further, the mass ratio of the active ingredients to the matrix is (42-53): (47-58).
Further, the active component further comprises a mildew preventive which is calcium propionate according to the mass part, and the addition amount of the mildew preventive is 1.5-1.8 parts according to the mass part.
A preparation method of a feeding emulsifier is used for the feeding emulsifier and comprises the following steps:
step A, dividing a substrate into three parts according to 30-40 parts, 2-8 parts and 10-15 parts by mass;
step B, uniformly mixing 30-40 parts of matrix, 25-50 parts of feeding lysolecithin and 5-20 parts of Tween to obtain a first premix;
step C, uniformly mixing 2-8 parts of matrix, 0.1-0.5 part of butyl hydroxy anisole and 0.1-0.5 part of ethoxyquinoline to obtain a second premix;
step D, uniformly mixing the first premix obtained in the step B, the second premix obtained in the step C and 10-15 parts of matrix to obtain a mixture;
and E, crushing the mixture obtained in the step D to obtain the feeding emulsifier.
In the step E, the mixture obtained in the step D is pulverized by a claw pulverizer or a blade pulverizer, and the mesh number of the pulverizer is 26 to 60 meshes.
Compared with the prior art, the embodiment of the invention has the following beneficial effects:
the method can improve the content of lysolecithin in the soybean lecithin, the enzymolysis yield of the lysolecithin is higher than that of the conventional enzymolysis process, so that a lysolecithin product for feeding is obtained, the content of the lysolecithin in the lysolecithin for feeding can reach 6 percent, the production cost is effectively reduced, the lysolecithin product can promote the digestive absorption of fat by livestock and poultry when being used as a feed additive, and the problems of low enzymolysis yield of the lysolecithin in the conventional soybean lecithin and high production cost of the lysolecithin are solved.
In addition, the active ingredient and the matrix are used in the feed emulsifier, wherein the matrix is used as a carrier of the active ingredient, the feed lysolecithin is adsorbed and dispersed, the feed lysolecithin is changed from oil into powder, and the matrix is used as a carrier and can be used as a filling agent, so that the content of the lysolecithin in a final product is stabilized, and the prepared feed emulsifier is conveniently added into the feed, so that the feed emulsifier and the feed can be uniformly mixed. The feeding emulsifier of the invention has high content of lysolecithin, and solves the problems that the existing feeding emulsifier only simply compounds soybean lecithin, and the feeding emulsifier has low content of lysolecithin, so that the feeding emulsifier is inconvenient to add and use in feed.
Detailed Description
A preparation method of feeding lysolecithin comprises the following steps:
step (1), putting soybean phospholipids into an enzymolysis tank for preheating;
step (2), adding water;
adding phospholipase;
heating and keeping the enzymolysis temperature for enzymolysis;
and (5) after the enzymolysis is finished, heating to inactivate enzyme to obtain the feeding lysolecithin.
The method has the advantages that the content of the lysolecithin in the soybean lecithin is low, the content of the lysolecithin in the conventional soybean lecithin is about 0.1-0.2%, the soybean lecithin is used as a raw material, and the phospholipase is used for enzymolysis, so that the content of the lysolecithin in the soybean lecithin can be increased, the enzymolysis yield of the lysolecithin is higher than that of the conventional enzymolysis process, a feeding lysolecithin product is obtained, the content of the lysolecithin in the feeding lysolecithin can reach 6%, the production cost is effectively reduced, the method is used as a feed additive, the digestion and absorption of fat by poultry and livestock can be promoted, and the problems that the enzymolysis yield of the lysolecithin in the conventional soybean lecithin is low, and the production cost of the lysolecithin is high are solved.
Specifically, soybean lecithin or soybean lecithin powder can be used as a raw material for enzymolysis.
Specifically, in the step (1), the preheating temperature is 40-60 ℃, and the preheating time is 10-60 min;
in the step (4), the enzymolysis temperature is 50-70 ℃, and the enzymolysis time is 1.5-4 h;
in the step (5), the enzyme deactivation temperature is 90-100 ℃, and the enzyme deactivation time is 0.5-1 h.
Through injecing preheating temperature and preheating time, preheating temperature is close with enzymolysis temperature, and temperature regulation time when can reducing the enzymolysis guarantees that certain preheating time is in order to ensure that soybean lecithin has reached corresponding preheating temperature, injects enzymolysis temperature, enzymolysis time and enzyme deactivation temperature and enzyme deactivation time, can guarantee the enzymolysis effect to soybean lecithin.
In the step (2), the addition amount of water is 5-25% of the mass of the soybean lecithin;
in the step (3), the addition amount of phospholipase is 0.1-1.5% of the mass of the soybean phospholipids, and the phospholipase is phospholipase A1, phospholipase A2 or lipase.
The method has the advantages that the addition amount of water and the addition amount of phospholipase are limited, the enzymolysis effect of the soybean lecithin is guaranteed, and the phospholipase uses phospholipase A1, phospholipase A2 or lipase, so that the enzymolysis effect can be guaranteed, the content of lysolecithin in the soybean lecithin is effectively improved, and the enzymolysis yield of the lysolecithin is higher than that of the conventional enzymolysis process.
In the step (4), after the vitamin E is added, heating and maintaining the enzymolysis temperature for enzymolysis, wherein the addition amount of the vitamin E is 0.01-0.1% of the mass of the soybean phospholipids, so as to prevent the oxidation of the phospholipids at a high temperature.
The feeding lysolecithin is prepared by the preparation method of the feeding lysolecithin.
The method takes the soybean lecithin as a raw material, adopts the phospholipase for enzymolysis, can improve the content of the lysolecithin in the soybean lecithin, can make the content of the lysolecithin in the prepared feeding lysolecithin reach 6 percent, and solves the problem of low enzymolysis yield of the lysolecithin in the existing soybean lecithin.
The feeding lysolecithin is used as a feeding emulsifier, and the raw materials comprise, by mass, 30.2-71 parts of an active ingredient and 47-58 parts of a matrix, wherein the active ingredient comprises 25-50 parts of feeding lysolecithin, 5-20 parts of tween, 0.1-0.5 part of butyl hydroxy anisole and 0.1-0.5 part of ethoxyquinoline;
the substrate is selected from one or a combination of two of rice bran meal, corncob powder, unite bran, defatted rice bran, corn gluten meal, white carbon black, zeolite powder, bentonite, calcium carbonate and dolomite powder.
The feed emulsifier is prepared by using an active ingredient and a matrix in the feed emulsifier, wherein the matrix is used as a carrier of the active ingredient, the feed lysolecithin is adsorbed and dispersed, the feed lysolecithin is changed into powder from oil, and the matrix is used as a carrier and can be used as a filling agent, so that the content of the lysolecithin in a final product is stable, and the prepared feed emulsifier is conveniently added into feed for use, so that the feed emulsifier and the feed can be uniformly mixed.
The feeding emulsifier of the invention has high content of lysolecithin, and solves the problems that the existing feeding emulsifier only simply compounds soybean lecithin, and the feeding emulsifier has low content of lysolecithin, so that the feeding emulsifier is inconvenient to add and use in feed.
Preferably, the mass ratio of the active ingredients to the matrix is (42-53): (47-58).
The form and the stability of the feeding emulsifier can be ensured by limiting the mass ratio of the active ingredients to the matrix, so that the using effect of the feeding emulsifier is ensured.
Further, the active component further comprises a mildew preventive which is calcium propionate according to the mass part, and the addition amount of the mildew preventive is 1.5-1.8 parts according to the mass part.
The feed emulsifier has the advantages that the mildew-proof effect of the feed emulsifier can be further improved by adding the mildew preventive, and the quality stability of the feed emulsifier during storage can be further ensured by adding the antioxidant into the raw materials of the feed emulsifier and using the feed emulsifier together with the mildew preventive.
A preparation method of a feeding emulsifier is used for preparing the feeding emulsifier and comprises the following steps:
step A, dividing a substrate into three parts according to 30-40 parts, 2-8 parts and 10-15 parts by mass;
step B, uniformly mixing 30-40 parts of matrix, 25-50 parts of feeding lysolecithin and 5-20 parts of Tween to obtain a first premix;
step C, uniformly mixing 2-8 parts of matrix, 0.1-0.5 part of butyl hydroxy anisole and 0.1-0.5 part of ethoxyquinoline to obtain a second premix;
step D, uniformly mixing the first premix obtained in the step B, the second premix obtained in the step C and 10-15 parts of matrix to obtain a mixture;
and E, crushing the mixture obtained in the step D to obtain the feeding emulsifier.
The feed emulsifier is prepared by dividing a substrate into three parts, mixing feeding lysolecithin and tween with a part of the substrate to obtain a first premix, mixing butyl hydroxyanisole and ethoxyquinoline with a part of the substrate to obtain a second premix, and finally mixing the first premix and the second premix with the rest of the substrate, wherein the substrate is used as a carrier of active ingredients and can play a role in adsorption and dispersion, so that raw materials in the feed emulsifier are uniformly dispersed, and the content of lysolecithin in the finally obtained feed emulsifier is stable.
The feeding emulsifier prepared by the preparation method has high content of lysolecithin, and solves the problems that the existing feeding emulsifier has low content of lysolecithin and is inconvenient to add and use in feed.
And D, in the step C, a mildew preventive can be added, so that the mildew preventive effect of the feed emulsifier is further improved, the quality stability of the feed emulsifier in storage is ensured, and the addition amount of the mildew preventive is 0.5-1.5 parts by weight.
The feed emulsifier prepared by the invention is used for being added into daily ration of piglets to prepare piglet feed, and the dosage of the feed emulsifier is equivalent to 100 ppm-500 ppm of the mass of the piglet feed.
In the step E, the mixture obtained in the step D is pulverized by a claw pulverizer or a blade pulverizer, and the mesh number of the pulverizer is 26 to 60 meshes.
And E, by limiting the mesh number of the screen of the pulverizer in the step E, the active substances and the carrier can be uniformly mixed, the appearance of the prepared feed emulsifier is regular and consistent, if the mesh number is too small, the mixing uniformity is not enough, and if the mesh number is too large, the feed lysolecithin is easily intercepted by the screen, so that the content of the lysolecithin in the feed emulsifier is reduced.
In order to facilitate an understanding of the present invention, a more complete description of the present invention is provided below. The present invention may be embodied in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
The examples do not specify particular techniques or conditions, and are performed according to techniques or conditions described in literature in the art or according to the product specification. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products commercially available.
Example 1
A preparation method of feeding lysolecithin comprises the following steps:
step (1), putting 40kg of soybean lecithin into a 50L enzymolysis tank for preheating, wherein the preheating temperature is 40 ℃, and the preheating time is 60 min;
adding 8kg of water (specifically distilled water) into the enzymolysis tank, wherein the addition amount of the water is 20% of the mass of the soybean lecithin;
adding 0.6kg of phospholipase into the enzymolysis tank, wherein the addition amount of the phospholipase is 1.5% of the mass of the soybean lecithin;
heating and keeping the enzymolysis temperature, and carrying out enzymolysis, wherein the enzymolysis temperature is 50 ℃, and the enzymolysis time is 3 hours;
and (5) after the enzymolysis is finished, heating to inactivate enzyme, wherein the inactivating temperature is 90 ℃, and the inactivating time is 0.5h, so that the feeding lysolecithin is obtained.
Example 2
A preparation method of feeding lysolecithin comprises the following steps:
step (1), putting 40kg of soybean phospholipid into a 50L enzymolysis tank for preheating, wherein the preheating temperature is 50 ℃, and the preheating time is 30 min;
step (2), adding 6kg of water (specifically distilled water) into the enzymolysis tank, wherein the addition amount of the water is 15% of the mass of the soybean lecithin;
adding 0.4kg of phospholipase into the enzymolysis tank, wherein the addition amount of the phospholipase is 1% of the mass of the soybean phospholipids;
heating and keeping the enzymolysis temperature, and carrying out enzymolysis, wherein the enzymolysis temperature is 60 ℃, and the enzymolysis time is 2 hours;
and (5) after the enzymolysis is finished, heating to inactivate enzyme, wherein the inactivating temperature is 100 ℃, and the inactivating time is 0.5h, so that the feeding lysolecithin is obtained.
Example 3
A preparation method of feeding lysolecithin comprises the following steps:
step (1), putting 40kg of soybean lecithin into a 50L enzymolysis tank for preheating, wherein the preheating temperature is 60 ℃, and the preheating time is 20 min;
adding 4kg of water (specifically distilled water) into the enzymolysis tank, wherein the addition amount of the water is 10% of the mass of the soybean lecithin;
adding 0.2kg of phospholipase into the enzymolysis tank, wherein the addition amount of the phospholipase is 0.5 percent of the mass of the soybean lecithin;
step (4), heating and maintaining the enzymolysis temperature, and carrying out enzymolysis, wherein the enzymolysis temperature is 70 ℃, and the enzymolysis time is 1.5 hours;
and (5) after the enzymolysis is finished, heating to inactivate the enzyme, wherein the inactivating temperature is 100 ℃, and the inactivating time is 0.5h, so that the feeding lysolecithin is obtained.
Measuring an LPC value of feeding lysolecithin obtained by enzymolysis by using a liquid chromatograph, wherein LPC is an English abbreviation of lysophosphatidylcholine and represents the content of lysolecithin, the higher the content of LPC represents the better enzymolysis effect, soybean lecithin is a raw material, the content of lysolecithin in the conventional soybean lecithin is about 0.1-0.2%, and the detection results are shown in the following table:
TABLE 1 enzymolysis Effect of examples 1 to 3
From the test results, it can be seen that the content of lysolecithin in the feeding lysolecithin prepared in the examples 1 to 3 is as high as 6.10%, which is higher than that of the conventional soybean lecithin by about 0.1% to 0.2%, and the enzymolysis yield is high, so that the obtained feeding lysolecithin can be applied to feed additives, the digestion and absorption of fat by poultry and livestock are promoted, and the production cost of the feeding emulsifier is reduced.
Example 4
A preparation method of a feeding emulsifier comprises the following steps:
step A, calculating according to the mass parts (wherein the mass part of the following 1 part is 1kg), dividing a substrate into three parts according to 40 parts, 5 parts and 13 parts, wherein the substrate is 50 parts of white carbon black and 8 parts of dolomite powder;
step B, uniformly mixing 40 parts of matrix, 40 parts of feeding lysolecithin (specifically using the feeding lysolecithin prepared in example 1) and 10 parts of Tween 20 to obtain a first premix;
step C, uniformly mixing 5 parts of matrix, 0.3 part of butyl hydroxy anisole, 0.2 part of ethoxyquinoline and 1.8 parts of mildew preventive (the mildew preventive is specifically calcium propionate) to obtain a second premix;
step D, uniformly mixing the first premix obtained in the step B, the second premix obtained in the step C and 13 parts of matrix to obtain a mixture;
and E, crushing the mixture obtained in the step D by using a claw type crusher, wherein the mesh number of a screen of the crusher is 40 meshes, and thus obtaining the feeding emulsifier.
Example 5
A preparation method of a feeding emulsifier comprises the following steps:
step A, calculating according to the mass parts (wherein 1 part by mass is 1kg), dividing a substrate into three parts according to 40 parts, 5 parts and 13 parts, wherein the substrate is 40 parts of white carbon black and 18 parts of zeolite powder;
step B, uniformly mixing 40 parts of matrix, 40 parts of feeding lysolecithin (specifically using the feeding lysolecithin prepared in example 1) and 10 parts of Tween 20 to obtain a first premix;
step C, uniformly mixing 5 parts of matrix, 0.3 part of butyl hydroxy anisole, 0.2 part of ethoxyquinoline and 1.7 parts of mildew preventive (the mildew preventive is specifically calcium propionate) to obtain a second premix;
step D, uniformly mixing the first premix obtained in the step B, the second premix obtained in the step C and 13 parts of matrix to obtain a mixture;
and E, crushing the mixture obtained in the step D by using a claw type crusher, wherein the mesh number of a screen of the crusher is 40 meshes, and thus obtaining the feeding emulsifier.
Example 6
A preparation method of a feeding emulsifier comprises the following steps:
step A, calculating according to the mass parts (wherein 1 part by mass is 1kg), dividing a matrix into three parts according to 40 parts, 5 parts and 13 parts, wherein the matrix is 30 parts of white carbon black and 28 parts of defatted rice bran;
step B, uniformly mixing 40 parts of matrix, 40 parts of feeding lysolecithin (specifically using the feeding lysolecithin prepared in example 1) and 10 parts of Tween 20 to obtain a first premix;
step C, uniformly mixing 5 parts of matrix, 0.3 part of butyl hydroxy anisole, 0.2 part of ethoxyquinoline and 1.5 parts of mildew preventive (the mildew preventive is specifically calcium propionate) to obtain a second premix;
d, uniformly mixing the first premix obtained in the step B, the second premix obtained in the step C and 13 parts of matrix to obtain a mixture;
and E, crushing the mixture obtained in the step D by using a claw type crusher, wherein the mesh number of a screen of the crusher is 40 meshes, so as to obtain the feeding emulsifier.
The HLB value of the prepared feed emulsifier is measured by using the conventional HLB value measuring method, wherein the HLB value represents emulsifying capacity, the HLB is hydrophilic of a hydrophilic group/lipophilic of a lipophilic oil group, the larger the HLB value is, the stronger the hydrophilic property is, the smaller the HLB value is, the stronger the lipophilic property is, generally speaking, the HLB value is between 1 and 40, the hydrophilic-lipophilic turning point HLB is 10, the HLB is less than 10, the lipophilic property is more than 10, and the detection results are shown in the following table:
table 2 HLB value test results of examples 4 to 6
From the test results, the feeding emulsifying agents prepared in the embodiments 4 to 6 have good hydrophilicity, so that the feeding emulsifying agents are convenient to add into feeds for use, the content of lysolecithin in the feeding emulsifying agents is stable, and the feeding emulsifying agents can be uniformly mixed with the feeds as feed additives, and the using effect is good.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A preparation method of feeding lysolecithin is characterized by comprising the following steps:
step (1), putting soybean phospholipids into an enzymolysis tank for preheating;
step (2), adding water;
adding phospholipase;
step (4), heating and maintaining the enzymolysis temperature for enzymolysis;
and (5) after the enzymolysis is finished, heating to inactivate enzyme to obtain the feeding lysolecithin.
2. The preparation method of feeding lysolecithin according to claim 1, characterized in that in the step (1), the preheating temperature is 40-60 ℃ and the preheating time is 10-60 min;
in the step (4), the enzymolysis temperature is 50-70 ℃, and the enzymolysis time is 1.5-4 h;
in the step (5), the enzyme deactivation temperature is 90-100 ℃, and the enzyme deactivation time is 0.5-1 h.
3. The preparation method of feeding lysolecithin according to claim 1, characterized in that in the step (2), the addition amount of water is 5-25% of the mass of soybean lecithin;
in the step (3), the addition amount of phospholipase is 0.1-1.5% of the mass of the soybean phospholipids, and the phospholipase is phospholipase A1, phospholipase A2 or lipase.
4. The preparation method of feeding lysolecithin as claimed in claim 1, characterized in that in the step (4), after vitamin E is added, the mixture is heated and kept at an enzymolysis temperature for enzymolysis, and the addition amount of vitamin E is 0.01 to 0.1% of the mass of soybean lecithin.
5. A feeding lysolecithin, which is prepared by the method for preparing the feeding lysolecithin as claimed in any one of claims 1 to 4.
6. A feeding lysolecithin as claimed in claim 5, which is characterized by being prepared from 30.2-71 parts by weight of active ingredients and 47-58 parts by weight of matrix, wherein the active ingredients comprise 25-50 parts by weight of feeding lysolecithin, 5-20 parts by weight of Tween, 0.1-0.5 part by weight of butyl hydroxy anisole and 0.1-0.5 part by weight of ethoxyquinoline;
the substrate is selected from one or a combination of two of rice bran meal, corncob powder, unite bran, defatted rice bran, corn gluten meal, white carbon black, zeolite powder, bentonite, calcium carbonate and dolomite powder.
7. The feeding emulsifier as claimed in claim 6, wherein the mass ratio of the active ingredient to the matrix is (42-53): (47-58).
8. The feed emulsifier according to claim 6, wherein the active ingredient further comprises a mildew inhibitor, the mildew inhibitor is calcium propionate, and the addition amount of the mildew inhibitor is 1.5-1.8 parts by weight.
9. A method of preparing a feed emulsifier according to claim 6, comprising the steps of:
step A, dividing a substrate into three parts according to 30-40 parts, 2-8 parts and 10-15 parts by mass;
step B, uniformly mixing 30-40 parts of matrix, 25-50 parts of feeding lysolecithin and 5-20 parts of Tween to obtain a first premix;
step C, uniformly mixing 2-8 parts of matrix, 0.1-0.5 part of butyl hydroxy anisole and 0.1-0.5 part of ethoxyquinoline to obtain a second premix;
step D, uniformly mixing the first premix obtained in the step B, the second premix obtained in the step C and 10-15 parts of matrix to obtain a mixture;
and E, crushing the mixture obtained in the step D to obtain the feeding emulsifier.
10. The preparation method of the feeding emulsifier according to claim 9, wherein in the step E, the mixture obtained in the step D is crushed by using a claw crusher or a blade crusher, and the mesh number of the crusher is 26-60 meshes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210545605.3A CN114698734A (en) | 2022-05-19 | 2022-05-19 | Feeding lysolecithin and preparation method thereof, and feeding emulsifier and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210545605.3A CN114698734A (en) | 2022-05-19 | 2022-05-19 | Feeding lysolecithin and preparation method thereof, and feeding emulsifier and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114698734A true CN114698734A (en) | 2022-07-05 |
Family
ID=82177536
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210545605.3A Pending CN114698734A (en) | 2022-05-19 | 2022-05-19 | Feeding lysolecithin and preparation method thereof, and feeding emulsifier and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114698734A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103535526A (en) * | 2013-10-22 | 2014-01-29 | 广州智特奇生物科技股份有限公司 | Feed additive for improving fat utilization rate |
| CN103976150A (en) * | 2014-05-05 | 2014-08-13 | 潍坊柯能生物科技有限公司 | Fat emulsifier for feed use, preparation method and application thereof |
| CN105076669A (en) * | 2015-07-17 | 2015-11-25 | 东北农业大学 | Preparation method of soybean protein emulsion with high emulsibility |
| CN106173188A (en) * | 2015-05-07 | 2016-12-07 | 上海邦成生物工程有限公司 | A kind of composite oxidation preventive for fodder and preparation method thereof |
| CN109864178A (en) * | 2017-12-05 | 2019-06-11 | 广东百美倚德生物科技有限公司 | A kind of preparation and application of feeding microcapsules lysophosphatide powder |
-
2022
- 2022-05-19 CN CN202210545605.3A patent/CN114698734A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103535526A (en) * | 2013-10-22 | 2014-01-29 | 广州智特奇生物科技股份有限公司 | Feed additive for improving fat utilization rate |
| CN103976150A (en) * | 2014-05-05 | 2014-08-13 | 潍坊柯能生物科技有限公司 | Fat emulsifier for feed use, preparation method and application thereof |
| CN106173188A (en) * | 2015-05-07 | 2016-12-07 | 上海邦成生物工程有限公司 | A kind of composite oxidation preventive for fodder and preparation method thereof |
| CN105076669A (en) * | 2015-07-17 | 2015-11-25 | 东北农业大学 | Preparation method of soybean protein emulsion with high emulsibility |
| CN109864178A (en) * | 2017-12-05 | 2019-06-11 | 广东百美倚德生物科技有限公司 | A kind of preparation and application of feeding microcapsules lysophosphatide powder |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5043170A (en) | Animal feed composition containing a vitamin D metabolite | |
| KR101741728B1 (en) | Animal Feed additives and Method of Using Thereof | |
| AU2003233218A1 (en) | Method for reducing methane production emanating from the digestive activities of an animal | |
| CN103976150A (en) | Fat emulsifier for feed use, preparation method and application thereof | |
| Antoniou et al. | The utilization of rye by growing chicks as influenced by calcium, vitamin D3, and fat type and level | |
| CN109258935A (en) | Feed growth promotion oxidation-resistant compound additive and its preparation method and application | |
| CN104171417A (en) | Animal feed additive | |
| CN114698734A (en) | Feeding lysolecithin and preparation method thereof, and feeding emulsifier and preparation method thereof | |
| CN113730359A (en) | Fat-soluble vitamin solid particles and preparation method and application thereof | |
| US20160183557A1 (en) | Dietary paste compositions for ruminants and methods of making and using the same | |
| CN110946213B (en) | Feed fat powder and processing method thereof | |
| JP3553126B2 (en) | Feed with good oxidation stability | |
| CN103535526B (en) | Feed additive for improving fat utilization rate | |
| US20050220978A1 (en) | Dispersible protein composition | |
| CN112493371A (en) | Feed additive based on lipase, preparation method and application thereof, and broiler feed | |
| CN113229369A (en) | sn-2 saturated fatty acid active structured lipid composition and preparation method and application thereof | |
| CN114794238A (en) | Infant formula liquid milk and preparation method thereof | |
| AU2019332696A1 (en) | Technology for water dispersible phospholipids and lysophospholipids | |
| CN110897040A (en) | Grease composition, feeding grease, and preparation method and application thereof | |
| CN114271398B (en) | Oil powder composition for chicken feed and preparation method and application thereof | |
| JP2006296370A (en) | Substitute milk composition | |
| WO2015016827A1 (en) | Dietary compositions for ruminants and containers for storing and dispensing same | |
| US2179917A (en) | Medicinal supplement and method of making | |
| JP2008125369A (en) | Pig farming feed and pig farming method using the same | |
| EP4233552A1 (en) | Emulsifier mixture |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220705 |
|
| RJ01 | Rejection of invention patent application after publication |